CN114703081B - Brevundimonas ST3CS3 and application thereof - Google Patents
Brevundimonas ST3CS3 and application thereof Download PDFInfo
- Publication number
- CN114703081B CN114703081B CN202210000216.2A CN202210000216A CN114703081B CN 114703081 B CN114703081 B CN 114703081B CN 202210000216 A CN202210000216 A CN 202210000216A CN 114703081 B CN114703081 B CN 114703081B
- Authority
- CN
- China
- Prior art keywords
- st3cs3
- brevundimonas
- strain
- plant
- phosphorus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a strain of BrevundimonasBrevundimonasolei) ST3CS3 with the preservation number of CGMCC No.24140 in the China general microbiological culture Collection center; the strain has the capabilities of fixing nitrogen and dissolving potassium, and has higher capabilities of dissolving phosphorus and producing indoleacetic acid; the strain is colonized in a plant body, can obviously promote plant growth, increase chlorophyll and nitrogen content of the plant, further reduce the use of chemical fertilizers, improve crop yield and have important significance in agricultural production.
Description
Technical Field
The invention belongs to the field of growth-promoting bacteria biotechnology, and particularly relates to Brevundimonas and application thereof in promoting plant growth.
Background
With the development of industrialization and modernization in China, the cultivated area is drastically reduced year by year. Since the 60 s of the 20 th century, the use of the fertilizer ensures the safety of national grains and plays an important role in increasing the yield and income of agriculture in China. However, excessive use of fertilizers not only affects the quality of agricultural products, but also causes great pressure on rural ecological environments. In recent years, the conventional chemical fertilizers (such as nitrogen, phosphorus and potassium compound fertilizers) have the problems of low utilization rate and abuse, and because the chemical fertilizers generally contain heavy metals, the unreasonable application of the chemical fertilizers in successive years can cause accumulation of the heavy metals in soil, endanger the sustainable development of soil structures and human society, and the blind application of the conventional chemical fertilizers can cause a series of negative effects such as yield reduction and the like, so that the reasonable application and even the application reduction of the conventional chemical fertilizers are urgent.
Plant growth-promoting bacteria (PGPR) refers to self-growing bacteria beneficial to crops. The effect of promoting the growth of crops directly means that certain plant promoting bacteria can synthesize certain substances (such as auxin and the like) which directly play a role in the growth and development of crops and/or change the form of certain invalid elements in soil so as to make the substances effective and further facilitate the absorption (such as nitrogen fixation, phosphorus dissolution and the like) of crops. The growth-promoting bacteria can also indirectly act on certain plants to inhibit or reduce the adverse effect of certain plant diseases on the growth and the development of crops and the yield. This has prompted scientists worldwide to actively explore and develop microbial fertilizers that are microbial-centric to replace (or replace part of) the use of fertilizers and pesticides.
The microbial fertilizer takes plant growth promoting bacteria as a core, is a novel environment-friendly fertilizer, has the characteristics of long acting, no toxicity, no pollution, energy conservation, low cost and the like, and has the effects of promoting the growth of crops, improving the yield and the quality of the crops, reducing the nitrate content in the crop products, improving the disease resistance of the crops, improving the soil fertility, reducing the using amount of chemical fertilizers, purifying the environment, maintaining ecological balance and the like. Microbial fertilizer mainly depends on microbial vital activities to generate enzymes and beneficial substances to play roles, nitrogen, phosphorus and potassium are elements necessary for plant growth, nitrogen in air, inorganic phosphorus in soil and potassium in silicate mineral form are difficult to be directly absorbed by plants, and the microbial enzymes and beneficial substances can convert substances which cannot be utilized into elements which can be directly utilized; indoleacetic acid (IAA) is plant endogenous auxin, and participates in the regulation and control of various physiological and biochemical processes such as plant cell growth, and the IAA produced by microorganisms plays an important role in plant microecological environment. Under the current situation that China faces energy crisis, resource shortage and environmental pollution, the microbial fertilizer is self-evident to the importance of modern agricultural development in China, the development of the microbial fertilizer can be said to be an important basic stone for the environment of a small number of cultivated lands, which is a real pollution-free novel fertilizer, accords with the sustainable development concept of China, and has wide application scenes as people know and demand on green pollution-free agricultural products are higher, and plays an important role in the agricultural sustainable development of China.
Disclosure of Invention
The invention provides a plant endophytic bacterium shortwave sheetCytophyte ST3CS3, classified and namedBrevundimonas oleiThe microbial strain is preserved in China general microbiological culture collection center (CGMCC) in 12 months and 20 days of 2021, the preservation number is CGMCC No.24140, and the preservation address is: the institute of microorganisms of national academy of sciences of China, no. 1, no. 3, north Chen West Lu, the Korean region of Beijing. The invention also aims to provide a new application of the plant endophytic bacteria Brevundimonas ST3CS3, namely application of the plant endophytic bacteria Brevundimonas ST3CS3 in promoting plant growth, which can be used for preparing biological agents or microbial fertilizers for promoting plant growth, wherein the Brevundimonas ST3CS3 has nitrogen fixation and potassium decomposition capabilities and higher phosphate dissolution and IAA production capabilities; the strain is colonized in a plant body, so that the plant growth can be obviously promoted, the chlorophyll and nitrogen contents of the strain can be increased, and the use of chemical fertilizers can be further reduced.
In order to achieve the above object, the present invention adopts the following technical measures:
1. collecting the invasive plant stellera chamaejasme of Shangri-la pasture in Yunnan provinceStellera chamaejasme L.) washing the plant sample under tap water;
2. respectively performing surface disinfection on the plant sample divided into an overground part and an underground part, firstly soaking the overground part and the underground part in ethanol solution with the volume concentration of 75% for 3-5 min, washing the overground part and the underground part with sterile water for 3-5 times, soaking the overground part and the underground part in sodium hypochlorite solution with the effective chlorine concentration of 5% for 2-3 min, washing the overground part and the underground part with sterile water for 3-5 times, and placing the overground part and the overground part on sterile filter paper to absorb water after washing; bacterial separation is carried out by adopting a mixed dilution flat plate method, 1g of plant sample with sterilized surface is ground into powder in sterile mortar, the powder is fully mixed with 9mL of sterile water, the mixture is transferred to LB culture medium for culture after gradient dilution, the culture is carried out for 10 days at 37 ℃, bacterial colony growth is observed every other day, then the bacterial colony is picked up, a plurality of endophyte bacterial strains are obtained after separation and purification, and the endophyte bacterial strains are prepared into bacterial suspension;
3. inoculating the separated endophytic strain to an inorganic phosphorus culture medium to judge the phosphorus dissolving capacity of the strain by a hydrolysis circle;
4. selecting a bacterial strain with stronger phosphorus dissolving capability, storing the bacterial strain on an LB inclined plane for standby, and naming the bacterial strain as ST3CS3;
5. identification of Strain ST3CS3
combining morphological characteristics and molecular identification results, and finally identifying the strain as BrevibacteriumBrevundimonas olei) The method comprises the steps of carrying out a first treatment on the surface of the The culture medium used for preserving and activating the strain is LB culture medium.
According to the invention, the short-wave monad ST3CS3 separated from stellera chamaejasme is subjected to a potting experiment, the influence of the short-wave monad ST3CS3 on the growth of tobacco seedlings is discussed, namely, the influence research of short-wave monad ST3CS3 inoculation on the growth of potted tobacco is performed, and a bacterial strain and theoretical research basis are provided for microbial fertilizers.
Compared with the prior art, the invention has the following beneficial effects:
the Brevibacterium ST3CS3 provided by the invention is derived from stellera chamaejasme, has the capabilities of fixing nitrogen, dissolving phosphorus, dissolving potassium and producing IAA, can effectively colonize in plant bodies, effectively dissolves insoluble potassium in plant rhizosphere soil, improves the nitrogen content in plants, further promotes the growth of the plants, reduces the use of chemical fertilizers, has simple culture operation and low cost, is safe to the environment, and is suitable for industrial production and market popularization and application.
Drawings
FIG. 1 shows colony morphology of Brevundimonas ST3CS3 on LB medium;
FIG. 2 shows the growth of Brevundimonas ST3CS3 on inorganic phosphorus solid medium;
FIG. 3 is a standard operating curve for a phosphorus standard solution;
FIG. 4 shows the results of determination of the phosphate solubilizing ability of Brevundimonas ST3CS3;
FIG. 5 shows the results of measuring the nitrogen fixation capacity of Brevundimonas ST3CS3;
FIG. 6 shows the results of measuring the potassium-decomposing ability of Brevundimonas ST3CS3;
FIG. 7 is a standard working curve for IAA standard solutions;
FIG. 8 is a graph showing the results of an assay for the ability of Brevundimonas ST3CS3 to secrete IAA;
FIG. 9 is the effect of Brevundimonas ST3CS3 on tobacco seedling growth;
FIG. 10 is the effect of Brevundimonas ST3CS3 on leaf chlorophyll content of tobacco seedlings.
Detailed Description
The technical scheme of the present invention will be described in further detail with reference to specific embodiments and drawings, but the present invention is not limited to the following technical scheme. Unless specifically stated otherwise, the reagents, methods and apparatus employed in the present invention are those conventional in the art. Those skilled in the art can make reference to various general specifications, technical and scientific literature or related specifications, manuals, etc. before the filing date of this invention.
Example 1: isolation, screening and identification of Brevundimonas ST3CS3
(1) Collecting a plant sample of a stellera chamaejasme which is a pasture advantage plant in Shangri of Yunnan province, and washing the sample under running water;
(2) Respectively surface sterilizing the plant sample into overground part and underground part, firstly soaking in 75% ethanol solution for 5min, washing with sterile water for 5 times, soaking in 5% sodium hypochlorite solution for 2min, washing with sterile water for 4 times, and placing on sterile filter paper to absorb water after washing; bacterial separation is carried out by adopting a mixed dilution flat plate method, 1g of plant sample with sterilized surface is ground into powder in sterile mortar, and is fully mixed with 9mL of sterile water, and the mixture is diluted in gradient to prepare 10 -3 、10 -4 、10 -5 And transferred to LB medium (Yeast extract 5g, pancreatic eggs)Bai 10g, 10g of sodium chloride, 14g of agar and 1L of distilled water with the pH of 7.0-7.2), culturing the mixture in a biochemical incubator at 37 ℃ for 10 days in an inverted mode, observing the mixture every other day, picking single bacterial colonies when bacterial colonies grow out, separating and purifying the single bacterial colonies to obtain a plurality of endophytic bacterial strains, and preparing the endophytic bacterial strains into bacterial suspensions respectively;
(3) Inoculating the separated endophytic strain to an inorganic phosphorus culture medium (10 g of glucose, 0.5g of ammonia sulfate, 0.3g of sodium chloride, 0.3g of potassium chloride, 0.3g of magnesium sulfate heptahydrate, 0.03g of ferrous sulfate heptahydrate, 1g of manganese sulfate tetrahydrate, 5g of tricalcium phosphate, 14g of agar, 1L of distilled water and pH of 7.0-7.5) to judge the phosphorus dissolving capacity of the strain by hydrolysis circle;
(4) Selecting a strain with the strongest phosphorus dissolving capacity, storing the strain on an LB inclined plane for standby, and naming the bacterial strain as ST3CS3;
(5) Identification of Strain ST3CS3
Example 2: determination of the phosphate-solubilizing ability of Brevundimonas ST3CS3
Dissolving phosphorus ring: inoculating the separated Brevundimonas ST3CS3 to an inorganic phosphorus solid culture medium, repeating three times at a constant temperature of 30 ℃ for 5 days at 4 inoculation points of each dish, observing and recording the growth condition of the strain, a phosphorus dissolving ring (D) and a colony diameter (D), wherein the larger the D/D is, the stronger the phosphorus dissolving capability is shown, the result is shown in figure 2, the Brevundimonas ST3CS forms a more obvious phosphorus dissolving ring on the inorganic phosphorus culture medium, the diameter of the phosphorus dissolving ring is 0.75cm, and the diameter of the colony is 0.3cm.
Phosphorus dissolution ability: brevundimonas ST3CS is inoculated into an inorganic phosphorus liquid culture medium with an inoculation amount of 4% after shaking culture for 15 hours at 30 ℃ and 180rpm in the LB liquid culture medium, and simultaneously, three replicates are set for each treatment by taking the same amount of LB liquid culture medium as a blank control, and the culture is carried out on the shaking culture medium at 180rpm at 30 ℃ for 6 days; the method for measuring the content of soluble phosphorus by adopting a molybdenum-antimony colorimetric method (the wavelength of a spectrophotometer is 700 nm) comprises the following specific steps:
(1) centrifuging the fermentation liquor at 10000rpm for 10min, placing 2.5mL of supernatant into a 50mL volumetric flask, adding 2 drops of 2, 6-dinitrophenol indicator, adjusting the pH to slightly yellow with 10% sodium hydroxide or 5% dilute sulfuric acid, adding 5mL of molybdenum-antimony color development inhibitor, and adding deionized water to a volume of 50mL;
(2) standing for 30min, and performing color comparison under 700nm of a spectrophotometer while measuring a blank control group;
(3) drawing a phosphorus standard curve at the same time in an experiment, respectively sucking 5 mug/mL of phosphorus standard solution 0, 2, 4, 6, 8 and 10mL into a 50mL volumetric flask, adding 2 drops of 2, 6-dinitrophenol indicator, regulating the pH to be slightly yellow by using 10% sodium hydroxide or 5% dilute sulfuric acid, adding 5mL of molybdenum-antimony color-resistant agent, adding deionized water to fix the volume to obtain 0, 0.2, 0.4, 0.6, 0.8 and 1.0 mug/mL of phosphorus standard series solution, standing for 30min, comparing color under 700nm of a spectrophotometer, drawing a standard working curve (figure 3), substituting absorbance value measured in the step (2) into the standard working curve, and obtaining 51mg/L (figure 4) of phosphorus-soluble amount of Brevundimonas ST3CS in inorganic phosphorus culture solution, which indicates that Breidimonas ST3CS has the capability of converting inorganic phosphorus into organic phosphorus, so that plants can absorb phosphorus more easily, and further promote plant growth.
Example 3: determination of Nitrogen fixation Capacity of Brevundimonas ST3CS3
Inoculating Brevundimonas ST3CS3 to a three-zone line on a solid culture medium of Abbe (disodium hydrogen phosphate 0.2g, magnesium sulfate heptahydrate 0.2g, sodium chloride 0.2g, calcium carbonate 5g, mannitol 10g, calcium sulfate 0.1g, agar 14g, distilled water 1L, pH 7.0), repeating for three times, and culturing at 30 ℃ for 5 days at constant temperature; if the strain can grow on the culture medium, the strain is proved to have nitrogen fixation capacity, otherwise, the strain has no nitrogen fixation capacity.
The results are shown in FIG. 5, from which it can be seen that Brevundimonas ST3CS3 can grow on Abbe's medium, demonstrating its ability to fix nitrogen, brevundimonas ST3CS3 can fix N in air 2 Is converted into effective nitrogen, which is helpful for the absorption of nitrogen by plants and further promotes the growth of plants.
Example 4: determination of potassium-decomposing ability of Brevundimonas ST3CS3
Inoculating Brevundimonas ST3CS3 to silicate solid culture medium (disodium hydrogen phosphate 2g, sucrose 5g, magnesium sulfate heptahydrate 0.5g, ferric chloride 0.005g, calcium carbonate 0.1g, potassium feldspar powder 1g, agar 14g, distilled water 1L, pH 7.0-7.5), repeating three times for 4 inoculation points per dish, and culturing at 30 ℃ for 5 days at constant temperature; if the strain forms a potassium-decomposing ring on the strain, the strain proves that the strain has potassium-decomposing capability, otherwise, the strain does not have potassium-decomposing capability.
As a result, as shown in FIG. 6, brevundimonas ST3CS forms a potassium-releasing ring on a silicate solid medium, the diameter of the potassium-releasing ring is 0.45cm, and the diameter of a colony is 0.35cm, which indicates that Brevundimonas ST3CS3 can convert insoluble potassium into effective potassium, and is helpful for absorbing potassium by plants, thereby promoting plant growth.
Example 4: determination of the ability of Brevundimonas ST3CS3 to secrete IAA
Brevundimonas ST3CS3 was cultured in LB liquid medium (containing L-tryptophan 0.1 mg/mL) at 30℃in the dark on a shaker at 120rpm for 24h, 1.5mL of the broth was centrifuged at 12000rpm for 10min, the pellet was removed, 0.5mL of the supernatant was added with an equal amount of Salkowski's reaction solution (1 mL of 0.5mol/L ferric chloride, 49mL of 35% perchloric acid), reacted in the dark for 30min, absorbance was measured at 530nm, and each group was repeated three times with the same treatment as above in the non-sterile medium as a control for zeroing; IAA standard solutions with concentrations of 0, 5.5, 11, 22, 44 and 88 mug/mL are used for measuring absorbance values by the method, a standard curve is drawn (figure 7), then absorbance values of an experimental group inoculated with Brevundimonas are substituted into the standard curve, the IAA secretion amount of Brevundimonas ST3CS3 is 145.6mg/L (figure 8), the Brevundimonas ST3CS3 synthesizes phytohormone IAA by taking L-tryptophan as a precursor, growth and proliferation of plant cells are stimulated, moisture and nutrients are effectively absorbed, and vital activities of plant bodies are regulated.
Example 5: growth promotion experiment of Brevundimonas ST3CS3 on tobacco seedlings
This example is intended to demonstrate the promoting effect of Brevundimonas ST3CS3 on plant growth; tobacco is used as raw materialNicotiana tabacumL.) are test plants, the experimental procedure is as follows:
A. preparation of tobacco aseptic seedlings: randomly selecting tobacco seeds (MSK 326), firstly soaking the tobacco seeds in ethanol solution with the volume concentration of 75% for 2min, and washing the tobacco seeds with sterile water for 4 times; then soaking in NaClO solution with 5% of available chlorine concentration for 1min, flushing with sterile water for 5 times, and placing on sterile filter paper to absorb water for later use; then autoclaving (121deg.C, 15psi,15 min; 3 times) the tray containing Canadian peat and perlite (volume ratio 7:3), placing tobacco seeds into the tray, germinating at 25deg.C and relative humidity of 60%, and watering the plants with distilled water every 3 days for retaining water;
B. preparation of endophyte inoculants and inactivators: inoculating Brevundimonas ST3CS3 into a 250mL conical flask, and culturing for 15h in a constant-temperature shaking table at 28 ℃ and 180 rpm; dividing the bacterial liquid into A, B part, taking part A as endophytic bacteria inoculant,
sterilizing part B at 121deg.C and 15psi for 15min to kill endophytes as inactivating agent;
C. potting experiment: after 30 days of tobacco seed planting, 20 seedlings with consistent growth vigor are selected and transplanted into flowerpots (10.5X8.5 cm;1 seedling/pot), each pot containing 600g of field soil and perlite (7:3, v/v) and 3g of calcium phosphate. The flowerpots are randomly divided into two groups (group I and group II), 10 pots of each group, then endophyte inoculant and inactivating agent of the step B are sprayed on leaf surfaces and roots of group I and group II seedlings respectively, and each plant is inoculated with 3mL until moist (group I: E+, ST3CS3 inoculation; group II: E-, ST3CS3 non-inoculation). Inoculating bacterial liquid on 1 st and 9 th days after transplanting, inoculating for 2 times, placing the seedlings at room temperature (18-25 ℃) for natural illumination culture, pouring sterile water every 3 days during the culture period, pouring 100mL of water into each pot (preferably, the water is poured through soil without overflowing the bottom of the pot), and closely observing the growth condition of each group of tobacco seedlings in the experimental process. To determine bacterial colonization in inoculated plants, 3 plants were randomly collected in the E+ and E-groups 5 days after the last inoculation, washed under tap water and surface sterilized, respectively; then, seedlings were homogenized by grinding, and the diluted suspension was cultured in LB medium to examine the inoculated strain. The strain was re-isolated from E+ plants, but not from E-plants, confirming the colonization by Brevundimonas. After 25 days of cultivation, plants were harvested and the height, dry weight, root length, leaf width, leaf length and chlorophyll content of the plants, and the nitrogen, phosphorus and potassium content in the soil were determined.
The results are shown in tables 1, 2, and fig. 9 and 10, and the results show that Brevundimonas ST3CS3 has a significant promoting effect on tobacco seedlings, and significant differences (p <0.01, t-test) are generated in both experimental and control groups. At 25 days, plant height, leaf length, leaf width, above-ground dry weight, below-ground dry weight, plant nitrogen content and chlorophyll content were increased by 43.36%,27.63%,25.17%,56.90%,74.84%,20.20%,15.06%, respectively, as compared to the control;
TABLE 1 Effect of Brevundimonas ST3CS3 on tobacco biomass
Note that: b represents a significant difference from a (p <0.01, t-test) on the basis of a;
TABLE 2 influence of Brevundimonas ST3CS3 on the nitrogen, phosphorus and Potassium contents in tobacco and its soil
Note that: b represents a significant difference from a (p <0.01, t-test) on the basis of a;
the results of the above examples demonstrate that Brevundimonas ST3CS3 isolated and obtained in the present invention has the ability to fix nitrogen, dissolve phosphorus and remove potassium, thereby promoting the absorption of nitrogen, phosphorus and potassium elements by plants, and also has IAA production ability, providing plant hormones to promote plant growth. The potting experiment proves that the growth of host plants can be improved after the Brevundimonas ST3CS3 is inoculated, and the method can be widely applied to the preparation of microbial fertilizers and can reduce the use and pollution of fertilizers.
Sequence listing
<110> university of Kunming engineering
<120> Brevundimonas ST3CS3 and application thereof
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1020
<212> DNA
<213> stellera chamaejasme (Stellera chamaejasme L.)
<400> 1
gggggaaggg ctagaatgca gtcgaacgaa ctcttcggag ttagtggcgg acgggtgagt 60
aacacgtggg aacgtgcctt ttggttcgga ataactcagg gaaacttgtg ctaataccga 120
atgtgccctt cgggggaaag atttatcgcc attagagcgg cccgcgtctg attagctagt 180
tggtgaggta acggctcacc aaggcgacga tcagtagctg gtctgagagg atgatcagcc 240
acactgggac tgagacacgg cccggactcc tacgggaggc agcagtgggg aatcttgcgc 300
aatgggcgaa agcctgacgc agccatgccg cgtgaatgat gaaggtctta ggattgtaaa 360
attctttcac cggggacgat aatgacggta cccggagaag aagccccggc taacttcgtg 420
ccagcagccg cggtaatacg aagggggcta gcgttgctcg gaattactgg gcgtaaaggg 480
cgcgtaggcg gatcgttaag tcaggggtga aatcccgggg ctcaacctcg gaattgccct 540
tgatactggc gatcttgagt atgagagagg tatgtggaac tccgagtgta gaggtgaaat 600
tcgtagatat tcggaagaac accagtggcg aaggcgacat actggctcat tactgacgct 660
gaggcgcgaa agcgtgggga gcaaacagga ttagataccc tggtagtcca cgccgtaaac 720
gatgattgct agttgtcggg ctgcatgcag ttcggtgacg cagctaacgc attaagcaat 780
ccgcctgggg agtacggtcg caagattaaa actcaaagga attgacgggg gcccgcacaa 840
gcggtggagc atgtggttta attcgaagca acgcgcagaa ccttaccacc ttttgacatg 900
cctggaccgc cacggagacg tggctttccc ttcggggact aggacacagg tgctgcatgg 960
ctgtcgccag ctcgggcctg ggaatgttgg gttaagtccc gaacgagcgc accctcgcct 1020
Claims (3)
1. Brevundimonas strainBrevundimonasolei) ST3CS3 is a plant endophytic bacterium separated from stellera chamaejasme, and the preservation number of the plant endophytic bacterium in the China general microbiological culture Collection center is CGMCC No.24140.
2. Use of Brevundimonas ST3CS3 according to claim 1 for promoting plant growth, wherein: brevundimonas ST3CS3 has the capabilities of phosphate and potassium dissolving, nitrogen fixing and indoleacetic acid production.
3. The use according to claim 2, characterized in that: brevundimonas ST3CS3 is used for preparing biological microbial agents or fertilizers for promoting plant growth.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210000216.2A CN114703081B (en) | 2022-01-03 | 2022-01-03 | Brevundimonas ST3CS3 and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210000216.2A CN114703081B (en) | 2022-01-03 | 2022-01-03 | Brevundimonas ST3CS3 and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114703081A CN114703081A (en) | 2022-07-05 |
| CN114703081B true CN114703081B (en) | 2023-04-28 |
Family
ID=82166458
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210000216.2A Active CN114703081B (en) | 2022-01-03 | 2022-01-03 | Brevundimonas ST3CS3 and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114703081B (en) |
Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015114552A1 (en) * | 2014-01-29 | 2015-08-06 | University Of Pretoria | Plant growth promoting rhizobacterial strains and their uses |
| CN104974947A (en) * | 2014-04-09 | 2015-10-14 | 吉林农业大学 | Brevundimonas sp. subspecies strain SZ-22 and application thereof |
| CN105385642A (en) * | 2015-12-29 | 2016-03-09 | 深圳市铁汉生态环境股份有限公司 | Brevundimonas and application thereof |
| CN106635910A (en) * | 2016-12-29 | 2017-05-10 | 长安大学 | Brevendimonas diminuta, bacterial agent and applications of microbial agent |
| CN110272841A (en) * | 2019-06-17 | 2019-09-24 | 甘肃省科学院生物研究所 | One plant of yellow shortwave monad and its application on informal voucher Radix Codonopsis |
| CN110468057A (en) * | 2019-09-02 | 2019-11-19 | 昆明理工大学 | One plants endogenetic disk both-end hair spore category fungi M7SB 41 and its application |
| CN110684700A (en) * | 2019-11-19 | 2020-01-14 | 曲阜师范大学 | Brevundimonas 2B and application thereof |
| CN110819563A (en) * | 2019-11-18 | 2020-02-21 | 曲阜师范大学 | Brevibacillus brevis 3C and application thereof in promoting growth of pleurotus eryngii |
| CN112094760A (en) * | 2020-10-13 | 2020-12-18 | 昆明理工大学 | Plant endophytic fungus Echinospora terrestris D2G24 and application thereof |
| CN112175887A (en) * | 2020-11-04 | 2021-01-05 | 塔里木大学 | Brevundimonas bacterium endophytic bacterium brevundimonas bacterium and application thereof |
| CN112358974A (en) * | 2020-12-09 | 2021-02-12 | 昆明理工大学 | Endophytic fungus epicoccum nigrum FZT214 and application thereof |
| CN112940992A (en) * | 2021-04-23 | 2021-06-11 | 安徽农业大学 | Tea tree endophytic bacteria with plant growth promoting effect, microbial inoculum and application thereof |
| CN113582769A (en) * | 2021-09-06 | 2021-11-02 | 西北工业大学深圳研究院 | Method for preparing organic fertilizer from kitchen garbage, organic fertilizer and application |
| CN113817653A (en) * | 2021-11-05 | 2021-12-21 | 昆明理工大学 | Pseudomonas fluorescens BsEB-1 and application thereof |
| CN113913328A (en) * | 2021-10-15 | 2022-01-11 | 内蒙古农业大学 | Plant rhizosphere growth-promoting bacterium and application thereof |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2916678C (en) * | 2013-06-26 | 2024-02-06 | Symbiota, Inc. | Seed-origin endophyte populations, compositions, and methods of use |
| WO2020132632A2 (en) * | 2018-12-21 | 2020-06-25 | Pivot Bio, Inc. | Methods, compositions, and media for improving plant traits |
| CA3128253A1 (en) * | 2019-02-05 | 2020-08-13 | Pivot Bio, Inc. | Improved consistency of crop yield through biological nitrogen fixation |
-
2022
- 2022-01-03 CN CN202210000216.2A patent/CN114703081B/en active Active
Patent Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015114552A1 (en) * | 2014-01-29 | 2015-08-06 | University Of Pretoria | Plant growth promoting rhizobacterial strains and their uses |
| CN104974947A (en) * | 2014-04-09 | 2015-10-14 | 吉林农业大学 | Brevundimonas sp. subspecies strain SZ-22 and application thereof |
| CN105385642A (en) * | 2015-12-29 | 2016-03-09 | 深圳市铁汉生态环境股份有限公司 | Brevundimonas and application thereof |
| CN106635910A (en) * | 2016-12-29 | 2017-05-10 | 长安大学 | Brevendimonas diminuta, bacterial agent and applications of microbial agent |
| CN110272841A (en) * | 2019-06-17 | 2019-09-24 | 甘肃省科学院生物研究所 | One plant of yellow shortwave monad and its application on informal voucher Radix Codonopsis |
| CN110468057A (en) * | 2019-09-02 | 2019-11-19 | 昆明理工大学 | One plants endogenetic disk both-end hair spore category fungi M7SB 41 and its application |
| CN110819563A (en) * | 2019-11-18 | 2020-02-21 | 曲阜师范大学 | Brevibacillus brevis 3C and application thereof in promoting growth of pleurotus eryngii |
| CN110684700A (en) * | 2019-11-19 | 2020-01-14 | 曲阜师范大学 | Brevundimonas 2B and application thereof |
| CN112094760A (en) * | 2020-10-13 | 2020-12-18 | 昆明理工大学 | Plant endophytic fungus Echinospora terrestris D2G24 and application thereof |
| CN112175887A (en) * | 2020-11-04 | 2021-01-05 | 塔里木大学 | Brevundimonas bacterium endophytic bacterium brevundimonas bacterium and application thereof |
| CN112358974A (en) * | 2020-12-09 | 2021-02-12 | 昆明理工大学 | Endophytic fungus epicoccum nigrum FZT214 and application thereof |
| CN112940992A (en) * | 2021-04-23 | 2021-06-11 | 安徽农业大学 | Tea tree endophytic bacteria with plant growth promoting effect, microbial inoculum and application thereof |
| CN113582769A (en) * | 2021-09-06 | 2021-11-02 | 西北工业大学深圳研究院 | Method for preparing organic fertilizer from kitchen garbage, organic fertilizer and application |
| CN113913328A (en) * | 2021-10-15 | 2022-01-11 | 内蒙古农业大学 | Plant rhizosphere growth-promoting bacterium and application thereof |
| CN113817653A (en) * | 2021-11-05 | 2021-12-21 | 昆明理工大学 | Pseudomonas fluorescens BsEB-1 and application thereof |
Non-Patent Citations (8)
| Title |
|---|
| Gang Wang等.Contributions of Beneficial Microorganisms in Soil Remediation and Quality Improvement of Medicinal Plants.Plants.2022,第11卷第1-33页. * |
| YULIANA SUSANTI等.The potential of endophytic bacteria from the root of Eucalyptus pellita as a biocontrol agent against Ralstonia solanacearum.BIODIVERSITAS.2021,第22卷(第6期),第3454-3462页. * |
| 刘丹丹 ; 李敏 ; 刘润进 ; .我国植物根围促生细菌研究进展.生态学杂志.2016,(第03期),第815-824页. * |
| 初龙 ; 李伟 ; 李欣亚 ; 熊帜 ; 李海燕 ; .重金属超富集植物种子内生真菌多样性及其重金属抗性.江苏农业学报.2017,(第01期),第43-49页. * |
| 杜文静 ; 刘锦霞 ; 李晶 ; 李娜 ; 丁品 ; 武建荣 ; 张建军 ; .甘肃地区道地药材根际固氮微生物分离鉴定.土壤与作物.2017,(第01期),第67-72页. * |
| 江悦娟.瑞香狼毒内生细菌多样性及其与宿主竞争力的相关性研究.中国优秀硕士学位论文全文数据库基础科学辑.2022,第1-79页. * |
| 谢远国 ; 王雅楠 ; 刘畅 ; 王宏飞 ; .盐生植物碱蓬二型果实表生细菌的群落组成及促生属性.微生物学通报.2018,(第07期),第1426-1437页. * |
| 谷青等.一株降解微囊藻毒素的缺陷短波单胞菌的分离鉴定 及降解特性研究.环境科学学报.2016,第37卷(第1期),第201-206页. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114703081A (en) | 2022-07-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR20110102787A (en) | Bacillus aryabhattai ls9 promoting the growth of plants and use thereof | |
| CN103146610A (en) | Plant growth-promoting rhizobacteria and application thereof | |
| CN110564637A (en) | Composite microbial inoculum for promoting wheat growth and application thereof | |
| CN115960744B (en) | Bacillus licheniformis and application thereof | |
| CN114276965A (en) | Bacillus belgii, suspension, preparation method and application | |
| US20230088888A1 (en) | Phosphorus-dissolving bacterium rbc25 regulated by phosphate deficiency and application thereof | |
| CN115029278A (en) | Bacillus megaterium and application thereof in promoting crop growth | |
| CN113278553B (en) | Enhanced efficient nitrogen fixation composite bacterial system added with non-nitrogen fixation bacteria and application thereof | |
| CN107467075B (en) | Application of bacillus pumilus as rice growth promoter | |
| CN114107130A (en) | Compound microbial fertilizer and preparation method and application thereof | |
| CN110122485A (en) | A kind of trichoderma, Trichoderma and preparation method thereof | |
| CN116904361B (en) | Copper bacteria strain with phosphate dissolving capability and application thereof | |
| CN116121147B (en) | Chenopodium ambrosioides seed endophytic Larimol agrobacterium and application thereof | |
| CN113088471A (en) | Rhizobium prazobium X2 for producing IAA and CMC enzymes and application thereof | |
| CN116925979A (en) | Bacillus bailii and application thereof in promoting plant growth | |
| CN116426445A (en) | Pseudomonas bacteria NJAU-T102 and application thereof | |
| CN114703081B (en) | Brevundimonas ST3CS3 and application thereof | |
| CN115725472A (en) | Pantoea agglomerans ZF15004, microbial inoculum, liquid bacterial fertilizer, preparation method, application and application method | |
| CN115725419A (en) | Phosphorus-dissolving blueberry endophytic trichoderma and application thereof | |
| CN111793585B (en) | Lactobacillus buchneri, culture method thereof and application thereof in agricultural planting | |
| CN111849842B (en) | Potassium bacteria, potassium bacteria microbial inoculum comprising same and application | |
| CN113897316A (en) | Bacillus licheniformis BLc06, functional melon and fruit seedling biological matrix prepared from same and application of functional melon and fruit seedling biological matrix | |
| CN111778197A (en) | Methylobacterium strain with high growth promoting efficiency and application thereof | |
| CN114774311B (en) | Flavobacterium and application thereof in promoting crop growth and improving crop cold resistance | |
| CN117645954B (en) | Paenibacillus mucilaginosus MSSW03 and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |