CN110468057A - One plants endogenetic disk both-end hair spore category fungi M7SB 41 and its application - Google Patents
One plants endogenetic disk both-end hair spore category fungi M7SB 41 and its application Download PDFInfo
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Abstract
The invention discloses a plants endogenetic disk both-end hair spore category fungi (SeimatosporiumSp.) 41 M7SB are CGMCC NO.18114 in the deposit number of China Committee for Culture Collection of Microorganisms's common micro-organisms center；The bacterial strain can be applied to the prevention and treatment of Powdery Mildew in Tobacco, to by two spore powdery mildews (Erysiphe cichoracearum DC Powdery Mildew in Tobacco caused by) has preferable antagonism, and can remarkably promote tobacco growing of catching an illness, and improves its disease resistance ability；The present invention has broad application prospects in biological control powdery mildew field, provides microorganism resource and theoretical foundation for the development and application of powdery mildew biological prevention and control agent.
Description
Technical field
The invention belongs to technical field of plant disease biological control, and in particular to a plants endogenetic disk both-end hair spore belongs to true
Bacterium (SeimatosporiumSp.) M7SB 41 and its application.
Background technique
Powdery Mildew in Tobacco (Tobacco Powdery Mildew) is one of Major Diseases of leaf tobacco production, it is by two spores
Powdery mildew (Erysiphe cichoracearum DC. fungal disease caused by) infecting, the whole country is interior generation.Disease hair
Position is spread from bottom to top usually since lower blade, and blade all falls off when serious, only stay vein (Guo Qitang etc.,
2009).Color is brown after sick leaf baking, and there is obvious dotted scab on blade face, and thin as a piece of paper, oil is few, and there is a serious shortage of miscellaneous gas for fragrance
Weight, damaged degree is big, and broken rate is high, seriously affects the yield and quality (Liu Caiyun etc., 2018) of tobacco leaf.Currently, cultivating disease-resistant product
Kind reinforces field planting management, using chemical control is major measure (Cai Lin for preventing and treating Powdery Mildew in Tobacco disease etc., and 2017).
However, the long-time service of chemical agent can not only enhance pathogen resistance, pesticide residue is caused to increase, influences smoker's safety,
Also ecological environment can be polluted and be destroyed.Therefore, people propose requirements at the higher level to the Prevention Technique of modern agriculture.Biology
Prevention and treatment because its is pollution-free, noresidue, it is at low cost the advantages that, be concerned in recent years (Li et al., 2015；Xie et
Al., 2016).About powdery mildew biological control, both at home and abroad it has been reported that and many bacterial strains realized commercially produce and
Good income is achieved in practical application.Such asAmpelomycesquisqualis Ces, Lecanicillium lecanii
Zimm, Bacillus subtilis, Bacillus velezensisCC09 andGjaerumia minorNyland etc.
In powdery mildew control significant effect (Nasir et al., 2014; Mmbaga et al., 2016; Cai et al.,
2017)。
Endophyte of plant (endophyte) refers to that one kind lives in health plant organization internal without causing plant tissue obvious
The microorganism (Arnold et al., 2000) that symptom changes.Since endophyte lives in inside plant tissues for a long time, with place
Main plant cooperated evolution, environment needed for plant provides growth for endophyte and nutriment, it is outstanding more that endophyte then passes through metabolism
Bioactive substance promote the growth and development of host plant, improve its resistivity to various biologies and abiotic stress
(Strobel & Daisy, 2003), wherein attracting attention most is endophyte enhancing host plant to various pathogens
Resistance (O ' Keeffe et al., 2017).Such as Mmbaga (2008) research discovery yeast endophyteRhodosporidiumSpp. can effective antagonism Fructus Corni powdery mildew generation.In recent years research finds endogenetic fungusMycosphaerella punctiformisIt may be Oak Tree powdery mildew pathogenic bacteriaErysiphe alphitoides It is effective
Antagonist (Jakuschkin, et al., 2016).However, research in relation to endophyte in terms of Powdery Mildew in Tobacco prevention and treatment compared with
It is few.Currently, both at home and abroad there is not yet application report of the disk both-end hair spore category fungi in Powdery Mildew in Tobacco prevention and treatment.
Summary of the invention
It is an object of that present invention to provide a plants endogenetic disk both-end hair spore category fungi (SeimatosporiumSp.) M7SB
41, China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number are preserved on July 26th, 2019
For CGMCC NO.18114, preservation address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica.
Another object of the present invention is to provide the new application of above-mentioned plant endogenesis disk both-end hair spore category fungi M7SB 41, i.e. needle
To the defect and deficiency of current Controlling Powdery Mildew technology, plant endogenesis disk both-end hair spore fungi M7SB 41 is applied and is being planted
In object controlling powdery mildew, disk both-end hair spore category fungi M7SB 41 provided by the present invention is experiments verify that have Powdery Mildew in Tobacco
There is preferable control efficiency, and the growth for the tobacco that can promote to catch an illness, enhances its disease resistance.
In order to achieve the goal above, the present invention takes following technical measures:
A, powder mildew resistance Wild Rosa multiflora Dali is pale reddish brown and the band leaf branch section of the susceptible Wild Rosa multiflora seven sisters of powdery mildew for acquisition, in from
It is rinsed well under water；
B, plant sample is divided into stem, leaf two parts carry out surface sterilization respectively, soaked first with the ethanol solution of volumetric concentration 75%
Steep 3 ~ 5min, aseptic water washing 3 ~ 5 times, then with effective chlorine density be 5% liquor natrii hypochloritis impregnate 2 ~ 3min, sterile water rush
It washes 3 ~ 5 times, places it in suck dry moisture on aseptic filter paper after rinsing；By stem, leaf texture's block be cut into segment and be affixed on containing
It is cultivated in the PDA culture medium of 0.5g/L streptomycin sulphate and 0.5g/L penicillin, 24 ~ 26 DEG C are cultivated 40 ~ 50 days, it observes every other day,
Seeing around tissue block has bacterium colony to grow then picking, endogenetic fungal bacterial strain is obtained after isolating and purifying, and endogenetic fungal bacterial strain is made
Bacteria suspension；
C, the sterile tobacco seedlings of potting are inoculated with endogenetic fungus bacteria suspension, later artificial challenge's powdery mildew, and it is outstanding that observation sprays powdery mildew spores
Influence of the endogenetic fungus to tobacco seedlings powdery mildew incidence after liquid 10 days is picked out to 4 ~ 5 plants of the preferable bacterial strain of powder mildew resistance
Secondary screening is carried out, bacterial strain M7SB 41 is obtained；
D, the identification of bacterial strain M7SB 41
41 morphological feature of M7SB: initial stage bacterium colony is in yellow-white, and subcircular is opaque, and later period culture bacterium colony is brown,
Bacterium colony is completely embedded with culture medium；Microscopically observation gleba is aceravlus, and subcircular organizes texture by the angle born of the same parents of brown
At top is irregularly drawn up；Conidiophore is cylindrical, elongated, has a small number of apical tiers to go out；Conidium spindle to bending,
General 4 cells, 2 cells of termination are colourless, and intermediate cell is dark-coloured, attached four Dan Sheng in top.
Molecular Identification: extracting the bacterial strain total DNA using kit, and sequencing company is sent to carry out sequencing after detecting,
Sequencing result is compared with sequence on NCBI；
Combining form feature and Molecular Identification are as a result, be finally accredited as Ascomycota, excrement shell Gammaproteobacteria, Xylaria sp. fungus for the bacterial strain
Mesh, circular hole shell section, disk both-end hair spore category (Seimatosporium) fungi.
Microorganism can be made in plant endogenesis disk both-end hair spore category fungi M7SB 41 and/or Metabolite of the present invention
Microbial inoculum.
The present invention applies plant endogenesis disk both-end hair spore category fungi M7SB 41 in Powdery Mildew in Tobacco prevention and treatment, described white
Powder disease be two spore powdery mildews (Erysiphe cichoracearum DC) caused by fungal disease；It is tested by greenhouse pot culture, it will
The sterile tobacco seedlings of potting are randomly divided into 2 groups, and every group of 10 repetitions, processing group is inoculated with the bacteria suspension of endophyte M7SB 41, and control group connects
The inactivation object (bacteria suspension sterilizes 20 minutes in 115 DEG C and is made) of 41 bacteria suspension of M7SB is planted, later artificial challenge's powdery mildew, respectively
Observation sprays powdery mildew spores suspension the 7th day, the 10th day endophyte bacterial strain M7SB 41 is to the shadow of tobacco seedlings powdery mildew incidence
It rings；Experimental result is shown: compared with the control group, the 7th day after either catching an illness or the 10th day, processing group (inoculating strain
M7SB 41) disease indexs of tobacco seedlings is substantially less than control group (bacterial strain M7SB 41 inactivate object), and bacterial strain M7SB 41 can be effectively controlled
The progression of the disease of tobacco seedlings powdery mildew.
In addition, bacterial strain M7SB 41 of the present invention can also remarkably promote infection powdery mildew tobacco growing, plant can be improved in adverse circumstance
Under the conditions of adaptability, tested by greenhouse pot culture, the sterile tobacco seedlings of potting be randomly divided into 2 groups, every group of 10 repetitions, processing group
It is inoculated with 41 bacteria suspension of endophyte bacterial strain M7SB, control group is inoculated with endophyte inactivation object, later artificial challenge's powdery mildew, respectively
In the chlorophyll content for spraying the two groups of plants of measurement in powdery mildew spores suspension the 7th day；And spraying powdery mildew spores suspension the 14th
Its harvesting tobacco seedlings, the biomass such as measurement plant height, root long, dry weight；Experimental result is shown: compared with the control group, the plant height of processing group,
Root long, dry weight and chlorophyll content all improve a lot.
Therefore disk both-end hair spore category fungi M7SB 41 provided by the present invention can be used as a kind of plant pathogenic fungi antagonism
Bacterium effectively inhibits the development of the plant powdery mildew state of an illness and remarkably promotes the growth for the plant that catches an illness, enhance its disease resistance, is plant
The prevention and treatment of powdery mildew provides new solution, has broad application prospects.
Compared with prior art, the invention has the following advantages:
(1) disk both-end hair spore category fungi M7SB 41 has apparent inhibition to make the Powdery Mildew in Tobacco state of an illness caused by two spore powdery mildews
With providing a kind of new biological control method for the prevention and treatment of plant powdery mildew, be inoculated in after catching an illness plant, plant can be remarkably promoted
Object growth, improves its disease resistance, is one plant of very potential disease-resistant bacterial strain；Bacterial strain M7SB 41 derives from powdery mildew highly resistance
The pale reddish brown stem in Wild Rosa multiflora Dali, long term growth is in plant, with host plant coevolution, mutualism；With it is traditional
It learns chemical control powdery mildew to compare, biological control is more environmentally-friendly, safe；
(2) it can be obtained the mycelium that largely can be used for controlling powdery mildew by simple Liquid fermentation, thallus easily obtains, at low cost
It is honest and clean, possess the potential of commercial applications.
Detailed description of the invention
Fig. 1 is colonial morphology of the disk both-end hair spore category fungi M7SB 41 in PDA culture medium, and wherein a figure is bacterial strain
M7SB 41 grows 7 days forms in PDA culture medium, b figure be bacterial strain M7SB 41 in PDA culture medium it is observed that gleba
When form；
Fig. 2 is processing group and control group Powdery Mildew in Tobacco incidence, and wherein a figure is control group, and b figure is processing group；
Fig. 3 is the influence being inoculated with after disk both-end hair spore category fungi M7SB 41 to the Powdery Mildew in Tobacco state of an illness；
Fig. 4 is the influence being inoculated with after disk both-end hair spore category fungi M7SB 41 to tobacco chlorophyll content.
Specific embodiment
Further details of the technical solution of the present invention with attached drawing combined with specific embodiments below, but the present invention is simultaneously
It is not limited to following technical scheme.Unless stated otherwise, the present invention uses reagent, method and apparatus is the art routines
Reagent, method and apparatus.Those skilled in the art be referred to the present patent application day before various common tool books,
Scientific and technical literature or relevant specification, handbook etc. are implemented.
Embodiment 1: separation, screening and the identification of disk both-end hair spore category fungi M7SB 41
1, herborization sample
Powder mildew resistance Wild Rosa multiflora Dali is pale reddish brown and the susceptible Wild Rosa multiflora seven sisters of powdery mildew to pick up from Dali City, yunnan Province grey
Mountain area；Sample is acquired by S-shaped, and three repetitions are arranged；The specific method of sampling are as follows: cut length about 0.5 with sterile scissors
The band leaf branch of m section 20, is immediately placed in sterile bag after acquisition, posts label, takes back laboratory and the interior completion for 24 hours after sampling
The mask work of endophyte；
2, the plant sample of above-mentioned acquisition is rinsed well under tap water, be divided into stem, leaf two parts and carry out surface sterilization:
First use volumetric concentration 75% alcohol solution dipping 3min, aseptic water washing 4 times, then with effective chlorine density for 5% hypochlorous acid
Sodium solution impregnates 2min, aseptic water washing 4 times, places it in suck dry moisture on aseptic filter paper after rinsing；By stem, leaf texture's block
It is cut into the segment of 0.5 × 0.5cm and is affixed in the PDA culture medium containing 0.5g/L streptomycin sulphate and 0.5g/L penicillin, 25
DEG C culture 45 days, observe every other day, seeing around tissue block has bacterium colony to grow then picking, and the Nei Shengzhen of the plant is obtained after isolating and purifying
52 plants of bacteria strain；
3, above-mentioned isolated endogenetic fungal bacterial strain is inoculated on PDA plate culture medium, after culture 7-10 days, to mycelia
After covering with plate, mycelium is scraped along planar surface with aseptic operation knife, it is sterile that 20mL is added after being shredded with aseptic operation scissors
In water, mixes, endophyte bacteria suspension is made；Bacteria suspension is equally divided into 2 parts, portion is directly as Inoculant, another is in 115
DEG C sterilizing 20min inactivation object is made；The tobacco K326 tobacco leaf of two spore powdery mildews is infected with sterile scissors clip, powdery mildew bacterial plaque is big
Small is 1.0 × 1.0cm, is dissolved in 250mL sterile water, and it is spare that two spore powdery mildews spore suspension is made；Tobacco K326 seed is by jade
Xi Zhongyan seed Co., Ltd provides, batch number: 20170301, it is saved backup in 4 DEG C.By VFertile soil: VPerlite=7:3's
Ratio prepares mixed soil, 121 DEG C of sterilizing 15min, repeats sterilizing 1 time every other day, sterilize altogether 3 times it is spare；
Choose 100, tobacco K326 seed at random, with the alcohol solution dipping 2min of volumetric concentration 75%, aseptic water washing 3 times,
Then 1min is impregnated with the liquor natrii hypochloritis that effective chlorine density is 5%, aseptic water washing 3 times, the seed cleaned is placed in sterile
Suck dry moisture on filter paper；The sterile mixed soil of 150g is added into sterile floral disc and paves, the seed after surface sterilization is uniform
Ground is sowed wherein, and (18-25 DEG C) at room temperature is placed in, and the natural photoperiod is sprouted, and every 3d pours 100mL sterile water.
When sterile tobacco seedlings grow 4 ~ 5 true leaves, tobacco seedlings are transplanted into the flowerpot that diameter is 14.5 cm (1/basin)
And 2 groups are randomly divided into, every group of 3 repetitions；The 7th day and the 14th day after tobacco seedlings transplanting, processing group tobacco seedlings were inoculated with endophyte
The bacteria suspension of M7SB 41, control group are inoculated with the inactivation object (121 DEG C of high pressure steam sterilization 15min) of the endophyte bacteria suspension, connect altogether
Kind twice, is uniformly applied to tobacco leaf front and back sides and ground rhizome part after specifically dipping bacteria suspension with aseptic cotton carrier.
In second of inoculation endophyte bacteria suspension or its 5th day after inactivating object, artificial challenge's powdery mildew is specially spraying
It is primary to be inoculated with two spore powdery mildews spore suspension；The 7th day observation powdery mildew incidence after spraying powdery mildew, by following standard
It scores every tobacco leaf, by following severity Scaling criterion calculation disease index.
Severity Scaling standard:
0 grade: blade disease-free spot；
1 grade: lesion area accounts for 5% or less entire blade area；
3 grades: lesion area accounts for the 6% ~ 10% of entire blade area；
5 grades: lesion area accounts for the 11% ~ 20% of entire blade area；
7 grades: lesion area accounts for the 21% ~ 40% of entire blade area；
9 grades: lesion area accounts for 41% or more and death of entire blade area.
Disease index=∑ [(the disease number of sheets × corresponding value of series at different levels)/(investigation total number of sheets × morbidity highest value of series)]
×100。
4,8 plants of the bacterial strain that antagonism is filtered out from 52 plants of (covering 21 endogenetic fungus Pseudomonas) endogenetic fungus is (flat
Equal disease index < 25), secondary screening is carried out after picking out 5 plants of the preferable bacterial strain of resistance, wherein the best bacterial strain of resistance is Nei Shengzhen
It is pale reddish brown to be isolated from resistance Wild Rosa multiflora Dali by bacterium M7SB 41；
The present embodiment was once screened with the effective strain that the excised leaf of powdery mildew rose did mildew-resistance early period, but was not screened
To functional bacterial strain；The possible reason is Erysiphe can in the case where in vitro culture in obligate biotroph bacterial parasite
Energy growth conditions are unsatisfactory.Secondly, certain bacterial strains play resistance, it may be necessary to could be sent out by the metabolic process of host plant
The effect of waving, therefore this example uses potted plant experiment, is screened by model plant tobacco to powdery mildew function stem.
5, endogenetic fungus M7SB 41 is identified
(1) Morphological Characteristics: the endogenetic fungal bacterial strain M7SB 41 being separated to is inoculated on PDA plate, sees Fig. 1, and a schemes in Fig. 1
Grow 7 days forms in PDA culture medium for bacterial strain M7SB 41, b figure be bacterial strain M7SB 41 in PDA culture medium it is observed that
Form when gleba.Initial stage bacterium colony is in yellow-white, and subcircular is opaque, and later period culture bacterium colony is brown, bacterium colony and culture medium
It is completely embedded；Microscopically observation gleba is aceravlus, and subcircular, by angle born of the same parents' organizational composition of brown, top is not advised
Then draw up；Conidiophore is cylindrical, elongated, has a small number of apical tiers to go out；Conidium spindle to bending, general 4 cells,
2 cells of termination are colourless, and intermediate cell is dark-coloured, attached four Dan Sheng in top.
(2) it Molecular Identification: after bacterial strain M7SB 41 is inoculated into PDA culture medium inclined-plane culture, send to Kunming is large and holds up biological section
Skill Co., Ltd is sequenced；It is largely mentioned using the novel quick fungal DNA that hundred Tyke Bioisystech Co., Ltd of Beijing produces
It after taking kit to extract the total DNA of the bacterial strain, is sequenced after PCR amplification, sequencing result utilizes Nucleotide on NCBI
BLAST carries out sequence alignment；Found when sequence alignment and NCBI on known sequence it is variant, homologous degree is 98%；
Combining form feature and Molecular Identification are as a result, be finally accredited as Ascomycota, excrement shell Gammaproteobacteria, Xylaria sp. fungus for the bacterial strain
Mesh, circular hole shell section, disk both-end hair spore category (Seimatosporium) bacterial strain.
Embodiment 2: preventive and therapeutic effect of the disk both-end hair spore category fungi M7SB 41 to Powdery Mildew in Tobacco
The present embodiment is intended to prove that disk both-end hair spore category bacterium M7SB 41 prevents the Powdery Mildew in Tobacco as caused by two spore powdery mildews
Effect is controlled, experimentation is as follows:
A, disk both-end hair spore category bacterial strain M7SB 41 preparation of 41 bacteria suspension of disk both-end hair spore category bacterial strain M7SB: is inoculated into PDA
On plating medium, 25 DEG C scrape mycelium along planar surface after constant temperature incubation 10 days, with aseptic operation knife, take 3g mycelium,
It is added in 20mL sterile water, mixes after being shredded with aseptic operation scissors, it is outstanding that 15g/100mL mycelial 41 bacterium of M7SB living is made
Liquid is spare；
B, prepared by two spore powdery mildews spore suspension: with the tobacco K326 tobacco leaf of sterile scissors clip infection two spore powdery mildews, white powder
Bacterium bacterial plaque size is 1.0 × 1.0cm, is dissolved in 250mL sterile water, it is spare that spore suspension is made；
C, greenhouse pot culture preventive effect is tested: tobacco K326 seed is purchased from China Tobacco Seed Yuxi Co., Ltd., batch number:
20170301, it is saved backup in 4 DEG C.By VFertile soil: VPerliteThe ratio of=7:3 prepares mixed soil, 121 DEG C of sterilizing 15min, every other day
Repeat sterilizing 1 time, altogether sterilize 3 times it is spare；Choose 100, tobacco K326 seed at random, first uses the ethanol solution of volumetric concentration 75%
Impregnate 2min, aseptic water washing 3 times, then with effective chlorine density be 5% sodium hypochlorite impregnate 1min, aseptic water washing 3 times,
Seed is placed in suck dry moisture on aseptic filter paper after flushing；The sterile mixed soil of 150g is added in sterile floral disc and paves, by table
Seed after the disinfection of face is equably sowed wherein, is placed in (18-25 DEG C) at room temperature, the natural photoperiod is sprouted, and every 3d is poured
100mL sterile water；
When sterile tobacco seedlings length to 4 ~ 5 true leaves, tobacco seedlings are transplanted into the flowerpot that diameter is 14.5 cm (1/basin) and random
It is divided into 2 groups, every group of 10 repetitions.The 7th day and the 14th day after tobacco seedlings transplanting, processing group tobacco seedlings were inoculated with endophyte M7SB 41
Bacteria suspension, control group is inoculated with the inactivation object (121 DEG C of high pressure steam sterilization 15min) of the endophyte bacteria suspension, altogether inoculation twice,
Tobacco leaf front and back sides and ground rhizome part are uniformly applied to after specifically dipping bacteria suspension with aseptic cotton carrier.In second of inoculation
The 5th day after raw bacterium bacteria suspension or its inactivation object, artificial challenge's powdery mildew, specific spray inoculation two spore powdery mildews spore suspension one
It is secondary；The 7th day, the 10th day observation powdery mildew incidence after spraying powdery mildew, the meter of state of an illness standards of grading and disease index
Formula is calculated with embodiment 1.
Greenhouse pot culture preventive effect experimental result is shown: after inoculation M7SB41 bacteria suspension, the disease index of tobacco seedlings is significantly reduced；
Control group is respectively 54.58 and 67.86 in inoculation the 7th day and the 10th day disease index of tobacco powdery mildew, and the disease of processing group
Feelings index is respectively 23.31 and 47.62.No matter it is inoculated with after powdery mildew the 7th day or the 10th day, handles and the incidence that compares
Exist significant difference (P< 0.001), inoculation M7SB41 significantly enhances the powder mildew resistance of tobacco seedlings, as shown in Figures 2 and 3.
Embodiment 3: influence of the inoculation disk both-end hair spore category bacterium M7SB 41 to tobacco growing of catching an illness
Experimentation and technical method are with embodiment 2, except that increasing cigarette while observing powdery mildew incidence
The measurement of seedling biomass；I.e. in the chlorophyll content for spraying powdery mildew spores suspension the 7th day measurement control group and processing group plant；
In spraying the 14th day harvesting tobacco seedlings of powdery mildew spores suspension, the biomass such as plant height, root long, dry weight are measured.
Potted plant experiment result is inoculated with 41 pairs of M7SB of disk both-end hair spore category bacterial strain infection powdery mildews as shown in table 1 and Fig. 4
Tobacco seedling growth has significant facilitation；Compared with the control group, the plant height of processing group, dry weight and chlorophyll content have larger
Improve, but the increase of root long it is not significant (P > 0.05), and the increase of plant height, dry weight and chlorophyll content reaches significant difference
(P < 0.01).
Table 1 is inoculated with influence of the disk both-end hair spore category bacterium M7SB 41 to tobacco growing of catching an illness
The result of above-mentioned case study on implementation illustrates that the disk both-end hair spore category bacteria strain M7SB 41 that acquisition is separated in the present invention can significantly press down
Powdery Mildew in Tobacco progression of the disease processed；Meanwhile being inoculated with the endogenetic fungus and can significantly promote the growth for the tobacco that catches an illness, enhance its disease-resistant energy
Power has achieved the purpose that effectively prevent Powdery Mildew in Tobacco, is one plant of bacterial strain for having very much potentiality to be exploited.
Claims (2)
1. a plants endogenetic disk both-end hair spore category fungi (SeimatosporiumSp.) 41 M7SB, in China Microbiological bacterium
The deposit number of kind preservation administration committee common micro-organisms center is CGMCC NO.18114.
2. application of the plant endogenesis disk both-end hair spore category fungi M7SB 41 described in claim 1 in plant powdery mildew prevention and treatment.
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