CN106635910A - Brevendimonas diminuta, bacterial agent and applications of microbial agent - Google Patents
Brevendimonas diminuta, bacterial agent and applications of microbial agent Download PDFInfo
- Publication number
- CN106635910A CN106635910A CN201611244659.7A CN201611244659A CN106635910A CN 106635910 A CN106635910 A CN 106635910A CN 201611244659 A CN201611244659 A CN 201611244659A CN 106635910 A CN106635910 A CN 106635910A
- Authority
- CN
- China
- Prior art keywords
- crude oil
- defect shortwave
- degradation
- defect
- monad
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000000813 microbial effect Effects 0.000 title abstract 5
- 230000001580 bacterial effect Effects 0.000 title description 16
- 239000010779 crude oil Substances 0.000 claims abstract description 65
- 241000894006 Bacteria Species 0.000 claims abstract description 31
- 239000007787 solid Substances 0.000 claims abstract description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 26
- 239000002689 soil Substances 0.000 claims abstract description 25
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 10
- 238000004321 preservation Methods 0.000 claims abstract 2
- 230000007547 defect Effects 0.000 claims description 56
- 239000000417 fungicide Substances 0.000 claims description 23
- 241000589539 Brevundimonas diminuta Species 0.000 claims description 18
- 244000005700 microbiome Species 0.000 claims description 11
- 230000000855 fungicidal effect Effects 0.000 claims description 10
- 230000000593 degrading effect Effects 0.000 claims description 9
- 239000002068 microbial inoculum Substances 0.000 claims description 6
- 108020004465 16S ribosomal RNA Proteins 0.000 claims description 5
- 238000006731 degradation reaction Methods 0.000 abstract description 35
- 230000015556 catabolic process Effects 0.000 abstract description 34
- 230000000694 effects Effects 0.000 abstract description 17
- 230000007613 environmental effect Effects 0.000 abstract description 3
- 238000005067 remediation Methods 0.000 abstract description 3
- 238000009629 microbiological culture Methods 0.000 abstract 1
- 239000003921 oil Substances 0.000 description 31
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 14
- 238000002835 absorbance Methods 0.000 description 14
- 238000000034 method Methods 0.000 description 14
- VZGDMQKNWNREIO-UHFFFAOYSA-N tetrachloromethane Chemical compound ClC(Cl)(Cl)Cl VZGDMQKNWNREIO-UHFFFAOYSA-N 0.000 description 12
- 238000010521 absorption reaction Methods 0.000 description 11
- 239000000725 suspension Substances 0.000 description 11
- 239000000243 solution Substances 0.000 description 10
- 239000002609 medium Substances 0.000 description 9
- 239000003208 petroleum Substances 0.000 description 9
- 239000001888 Peptone Substances 0.000 description 8
- 235000015278 beef Nutrition 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 7
- 229910052757 nitrogen Inorganic materials 0.000 description 7
- 239000000203 mixture Substances 0.000 description 6
- HIXDQWDOVZUNNA-UHFFFAOYSA-N 2-(3,4-dimethoxyphenyl)-5-hydroxy-7-methoxychromen-4-one Chemical compound C=1C(OC)=CC(O)=C(C(C=2)=O)C=1OC=2C1=CC=C(OC)C(OC)=C1 HIXDQWDOVZUNNA-UHFFFAOYSA-N 0.000 description 5
- 239000012153 distilled water Substances 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- RTZKZFJDLAIYFH-UHFFFAOYSA-N ether Substances CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 5
- 235000015097 nutrients Nutrition 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 235000010413 sodium alginate Nutrition 0.000 description 5
- 238000003756 stirring Methods 0.000 description 5
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 4
- 239000002028 Biomass Substances 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 238000011088 calibration curve Methods 0.000 description 4
- 150000001875 compounds Chemical group 0.000 description 4
- 239000000284 extract Substances 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 238000011084 recovery Methods 0.000 description 4
- 239000000661 sodium alginate Substances 0.000 description 4
- 229940005550 sodium alginate Drugs 0.000 description 4
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 108010080698 Peptones Proteins 0.000 description 3
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 3
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 3
- 239000001110 calcium chloride Substances 0.000 description 3
- 229910001628 calcium chloride Inorganic materials 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 150000002430 hydrocarbons Chemical group 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000009630 liquid culture Methods 0.000 description 3
- 239000005416 organic matter Substances 0.000 description 3
- 235000019319 peptone Nutrition 0.000 description 3
- 229910052698 phosphorus Inorganic materials 0.000 description 3
- 239000011574 phosphorus Substances 0.000 description 3
- 239000011591 potassium Substances 0.000 description 3
- 229910052700 potassium Inorganic materials 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 238000012163 sequencing technique Methods 0.000 description 3
- 238000010998 test method Methods 0.000 description 3
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 2
- 239000004215 Carbon black (E152) Substances 0.000 description 2
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 2
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 description 2
- 230000003213 activating effect Effects 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000004166 bioassay Methods 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000000356 contaminant Substances 0.000 description 2
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 2
- 229910052564 epsomite Inorganic materials 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 229930195733 hydrocarbon Natural products 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 239000006916 nutrient agar Substances 0.000 description 2
- 230000035479 physiological effects, processes and functions Effects 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- 238000012797 qualification Methods 0.000 description 2
- 239000012088 reference solution Substances 0.000 description 2
- 230000008439 repair process Effects 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Inorganic materials [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 2
- 210000001082 somatic cell Anatomy 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 229910052717 sulfur Inorganic materials 0.000 description 2
- 239000011593 sulfur Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 229930195727 α-lactose Natural products 0.000 description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- 241000772415 Neovison vison Species 0.000 description 1
- 241001052560 Thallis Species 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- 150000001335 aliphatic alkanes Chemical class 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 150000001491 aromatic compounds Chemical class 0.000 description 1
- 150000004945 aromatic hydrocarbons Chemical class 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000012876 carrier material Substances 0.000 description 1
- 239000009096 changqing Substances 0.000 description 1
- 239000003610 charcoal Substances 0.000 description 1
- 238000000205 computational method Methods 0.000 description 1
- 239000003431 cross linking reagent Substances 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 239000012259 ether extract Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
- 238000012215 gene cloning Methods 0.000 description 1
- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 description 1
- IXCSERBJSXMMFS-UHFFFAOYSA-N hydrogen chloride Substances Cl.Cl IXCSERBJSXMMFS-UHFFFAOYSA-N 0.000 description 1
- 229910000041 hydrogen chloride Inorganic materials 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229910052603 melanterite Inorganic materials 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- -1 nitrogenous heterocyclic compound Chemical class 0.000 description 1
- 231100000956 nontoxicity Toxicity 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000003973 paint Substances 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
- 230000001699 photocatalysis Effects 0.000 description 1
- 238000007146 photocatalysis Methods 0.000 description 1
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000004227 thermal cracking Methods 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 238000002137 ultrasound extraction Methods 0.000 description 1
- 238000000870 ultraviolet spectroscopy Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/32—Hydrocarbons, e.g. oil
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Environmental & Geological Engineering (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Water Supply & Treatment (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Soil Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to the technical field of bioengineering and particularly discloses Brevendimonas diminuta 1-8. The bacterium is preserved in the China General Microbiological Culture Collection Center with the preservation number being CGMCC No. 13002. The invention also discloses a solid microbial agent of the Brevendimonas diminuta 1-8 and applications of the solid microbial agent in degradation of crude oil contaminated water and crude oil contaminated soil. The Brevendimonas diminuta is easy to produce on a large scale and has high degradation efficiency and environmental adaptability. The bacterium and the particular microbial agent can show a better degradation effect in water contaminated by crude oil with concentration of 1 mL/100 mL, the solid microbial agent can also have good degradation and remediation effects on crude oil contaminated soil, can realize remediation treatment of crude oil pollution and has good application prospect in bioremediation of crude oil pollution.
Description
Technical field
The present invention relates to technical field of bioengineering, and in particular to a kind of defect shortwave monad, microbial inoculum and its application.
Background technology
The alkane for being included, aromatic hydrocarbon, sulfur-containing compound and sulfur-bearing or nitrogenous heterocyclic compound etc. in oil is
With toxicity, to the very harmful of the mankind and environment.It is natural organic substance through geology and crude oil is crude oil
A kind of sticky black for changing and being formed or dark-brown liquid or solid, are generally present in underground or sea mostly
Bottom, and with penetrating odor, main component is the chain compound that hydrocarbon atom is combined to form, and it is to water body, soil, air
Environment Deng human survival has greatly harm.
The recovery technique of water body, soil at present, to oil pollution etc. mainly has peripheral doses technology, chemical redemption skill
Art, photocatalysis recovery technique and bioremediation technology；Bioremediation technology is one and has policy, economic and technical attraction
Processing method, it is considered to be most potential technical method, and progressively develop into a kind of economic benefit and Environmental Effect
Benefit is all good, solve the maximally effective means of complex environment pollution problem.
A kind of microorganism is only capable of degraded one kind or a few crude oil hydrocarbon for universal, and different microorganisms are to pollution of the same race
The degradation effect of component is also different, and multiple-microorganism is in degradation process it is also possible that collaboration or Competition.But crude oil
Product is refined oil with it, contained crude oil Jing species is extremely complex, and crude oil its crude oil Jing compositions in the not homologous place of production also have very
Big difference.Therefore microorganism remediation technology also having some limitations property while developing rapidly, specific microorganism can only
Degrade specific type of compounds, breeding and the metabolic activity of microorganism are affected larger by environmental factor, usually add to
There is mortality in the starting stage of soil.
The content of the invention
For problems of the prior art, it is an object of the invention to provide one kind can be used for degrading crude oil contaminant water
The defect shortwave monad of body, the bacterial classification is directly added in polluted-water, reaches preferable degradation effect, realizes that crude oil is dirty
The improvement of dye.
In order to achieve the above object, the present invention is employed the following technical solutions and is achieved.
(1) a kind of defect shortwave monad, was preserved in BeiChen West Road, Chaoyang District, BeiJing City 1 on 09 12nd, 2016
The China Committee for Culture Collection of Microorganisms's common micro-organisms center of number No. 3 Institute of Microorganism, Academia Sinica of institute, protects
Tibetan number is CGMCC No.13002；It is named as defect shortwave monad (Brevundimonas diminuta) 1-8.
The defect shortwave monad is cultivated on beef-protein medium, bacterial strain 1-8 is in beef extract-peptone
Flat board in light yellow, with the prolongation of incubation time, colony colour is slightly deepened, and surface is smooth, moistening, and bacterium colony is in circle
Shape, central protrusion, neat in edge is presented translucent.Somatic cells are in single arrangement in quarter butt, bar-shaped, cell, and size is
0.5 μ m (0.1-0.3) μm, is Gram-negative bacteria.
The 16S rDNA sequencing result such as SEQ of defect shortwave monad (Brevundimonas diminuta) 1-8
Shown in ID No.1.
(2) a kind of defect shortwave unit cell bacteria agent, it is characterised in that the microbial inoculum is solid fungicide, and with defect shortwave
Monad (Brevundimonas diminuta) 1-8 is active component.
(3) application of the defect shortwave monad in degrading crude oil polluted-water.
(4) application of the defect shortwave unit cell bacteria agent in degrading crude oil polluted-water.
(5) application of the defect shortwave unit cell bacteria agent in degrading crude oil contaminated soil
Compared with prior art, beneficial effects of the present invention are：
The defect shortwave monad that the present invention is provided is not only easy to large-scale production, and degradation efficiency is high, and environment is adapted to
Property is strong.The defect shortwave monad for being provided can large amount of organic exist under conditions of smooth growth, with crude oil as carbon source, reach
To preferable degradation effect.The bacterium and its concrete microbial inoculum can embody in crude oil concentration up in the crude oil pollution water body of 1mL/100mL
Go out preferably degradation effect, its solid fungicide also there can be good degraded repairing effect to oil contaminated soil, realize crude oil
The repair process of pollution, crude oil pollution it is biological prosthetic in present good application prospect.
Description of the drawings
Below in conjunction with the accompanying drawings the present invention is described in further details with specific embodiment.
Fig. 1 is defect shortwave monad (Brevundimonas diminuta) 1-8 of the present invention in beef extract-peptone
Colonial morphology figure on culture medium flat plate；
Fig. 2 is that 1-8 is under the microscope for defect shortwave monad (Brevundimonas diminuta) of the invention
Thalli morphology figure, in figure, multiplication factor is 1600 times；
Fig. 3 is the wavelength-absorbance curve figure of I crude oil in the embodiment of the present invention 3；In figure, abscissa is wavelength, unit
For nm, ordinate is absorbance, and unit is 1；
Fig. 4 is the canonical plotting of I crude oil in the embodiment of the present invention 3；In figure, abscissa is concentration, and unit is mg/L,
Ordinate is absorbance, and unit is 1；
Fig. 5 is the canonical plotting of II crude oil in the embodiment of the present invention 4；In figure, abscissa is concentration, and unit is mg/
L, ordinate is absorbance, and unit is 1.
Specific embodiment
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will
It will be appreciated that the following example is merely to illustrate the present invention, and it is not construed as limiting the scope of the present invention.
Embodiment 1 bacterial strain is isolated and purified
(1) bacterium source
The present invention provides a kind of defect shortwave monad (Brevundimonas diminuta), is isolated from extending oil gathering
In seven inner hole oil recovery field oil gathering station ground crude oil contaminated soils of group, the physicochemical property of soil is：Ammonium nitrogen 15.09ppm, nitre state
Nitrogen 0.72ppm；Nitrogen content 15.81ppm, phosphorus content 82.05ppm, potassium content 17.45ppm, the content of organic matter 1.43%, pH is
7.65。
(2) bacterial strain is isolated and purified
10g oil-polluted soils sample is added in 100mL enriched liquid culture mediums, 28 DEG C, 130r/min shaking table cultures
7d.After nutrient solution muddiness, draw 5mL nutrient solutions and transfer again in fresh enriched medium, it is identical with above-mentioned condition of culture
Continuous switching enrichment culture 3 times.The above-mentioned bacteria suspensions of 1mL are taken, after being serially diluted with sterilized water, beef extract-peptone is inoculated in respectively
In culture medium, 29 DEG C or so incubated 3d.Wherein, the composition of enriched liquid culture medium is：NaNO31.5g, (NH4)2SO4
1.5g, K2HPO41g, MgSO4·7H2O 0.5g, KCl 0.5g, FeSO47H2O 0.01g, CaCl20.002g, distilled water
1000mL, I crude oil 1% (volume ratio), pH 7.0.Wherein, I crude oil picks up from the inner hole oil recovery field oil-collecting of prolongation Petroleum Group seven
Stand, density is 0.65g/mL；The composition of beef-protein medium is：Beef extract 3.0g, peptone 10.0g, NaCl
5.0g, nutrient agar 15.0g, distilled water 1000mL, pH 7.4-7.6.After flat-plate bacterial colony is efficiently separated, according to colony colour,
The difference of morphological feature and surface moisture, carries out plate streaking purifies and separates with transfer needle choosing colony respectively.By inoculation
In inclined-plane, 4 DEG C of refrigerator cold-storages are standby.
(3) molten scraper ring method screens oil degradation bacteria
Above-mentioned pure bacterium bacterial classification is inoculated on oil-containing inorganic salts solid medium, is placed in 37 DEG C of incubators and is cultivated 10-
15d, observation whether there is thermophilic scraper ring, and primarily determines that the oil drop of bacterial strain according to the ratio of thermophilic scraper ring diameter (D) and colony diameter (d)
Solution ability.The oil degradation bacteria of preliminary screening is inoculated in into slant medium, 4 DEG C of refrigerator cold-storages are standby.
The identification of the bacterial strain of embodiment 2
(1) morphological feature of bacterial strain
As shown in figure 1, by the defect shortwave monad after 30 DEG C of incubated 24h on beef-protein medium
Observation colonial morphology, bacterial strain 1-8 is in light yellow on the flat board of beef extract-peptone, with the prolongation of incubation time, bacterium colony face
Color is slightly deepened, and surface is smooth, moistening, and bacterium colony is rounded, and central protrusion, neat in edge is presented translucent.As shown in Fig. 2
In quarter butt, bar-shaped, cell is in single arrangement to somatic cells, and size is 0.5 μ m (0.1-0.3) μm.
(2) biochemical characteristic
According to《Primary Jie Shi Bacteria Identifications handbook》With《Common bacteria system identification handbook》Defect shortwave monad is carried out
Physiology and biochemistry identifies that qualification result is as shown in table 1.
The Physiology and biochemistry qualification result of the defect shortwave monad 1-8 bacterial strains of table 1
Note："-" represents that reaction is feminine gender；"+" represents that reaction is the positive.
(3) the 16S rDNA identifications of bacterial strain
The genomic DNA of bacterial strain 1-8 is extracted with DNA extraction kit, 16S rDNA gene clonings, sequencing is then carried out,
Its 16S rDNA sequencing result is specific as follows as shown in SEQ ID No.1：
Carry out Blast comparisons in GenBank afterwards, as a result show, with its sequence similarity reach 98.56% be it is scarce
Sunken shortwave monad (Brevundimonas diminuta), is attributed to unit cell mushroom and is named as defect shortwave monad
(Brevundimonas diminuta)1-8。
Defect shortwave monad (Brevundimonas diminuta) drops of the 1-8 to I crude oil pollution water bodys of embodiment 3
Solution
Test method：
(1) preparation of defect shortwave monad (Brevundimonas diminuta) 1-8 bacteria suspensions
Aseptically, picking isolates and purifies the oil degradation bacterium 1-8 of acquisition, is inoculated in the beef extract albumen of 100mL
In peptone fluid nutrient medium, shaking table Amplification Culture culture 48h；Wherein, the composition of beef extract-peptone fluid nutrient medium is：Beef extract
3.0g, peptone 10.0g, NaCl 5.0g, nutrient agar 15.0g, distilled water 1000mL, pH 7.4-7.6.PH value 1mol/L
NaOH or 1mol/L hydrogen chloride be adjusted to required value.Medium sterilization condition is：Temperature is 121～126 DEG C, and the time is
25min。
(2) measure of I crude oil maximum absorption wavelength
Oil and products thereof has certain Absorption Characteristics in ultraviolet region, the aromatic compound with phenyl ring in petroleum component
Object wave length is generally 250～260nm, and carries the compound wavelength of conjugated double bond typically between 215～230nm, there is research
Show, when being determined using ultraviolet spectrometry light method, 255nm or so be crude oil and the range of choice of mink cell focus, 225nm or so with for
The range of choice of the oil product of light oil and oil plant.Under 100mg/L concentration, according to the wavelength and absorbance data that obtain, paint
System supplies the wavelength-absorbance curve of examination I crude oil as shown in figure 3, wherein, and I crude oil picks up from the inner hole of prolongation Petroleum Group seven and adopts
Oily field oil gathering station, density is 0.65g/mL.
(3) drafting of I crude oil calibration curve
Take 0 respectively, 2,2.5,5,6,7mg I crude oil in 50mL colorimetric cylinders, use petroleum ether constant volume, concentration point now
Not Wei 0,40,50,100,120,140mg/L, under 258nm wavelength, with petroleum ether as reference solution, the suction of bioassay standard concentration
Luminosity, abscissa is concentration (mg/L), and ordinate is absorbance, draws the mark of concentration (the mg/L)-absorbance (A) of I crude oil
Directrix curve is as shown in Figure 4.
(4) measure of oil degradation rate
The 1-8 bacteria suspension 3mL of Amplification Culture are taken, in being inoculated in 100mL oil-containing inorganic salt liquid culture mediums, oil-containing inorganic salts
The composition of culture medium is：NaNO31.5g, (NH4)2SO41.5g, K2HPO41g, MgSO4·7H2O 0.5g, KCl 0.5g,
FeSO4·7H2O 0.01g, CaCl20.002g, distilled water 1000mL, I crude oil 1.5mL, pH 7.0；At 30 DEG C, 120rpm
7d is cultivated in thermostatic control oscillator vibration.Nutrient solution uses 5:It is 2-3 that the sulfuric acid solution of 1 (V/V) adjusts pH value, adds methyl alcohol 2-4mL,
Extracted three times with (60-90 DEG C) of the petroleum ether of 3 × 15mL, extract proceeds to 50mL volumetric flasks Jing after anhydrous sodium sulfate drying and determines
Hold.
By petroleum ether extraction liquid Jing after being serially diluted, with petroleum ether as reference, by its phase of determined by ultraviolet spectrophotometry
The absorbance answered, according to the calibration curve of concentration (the mg/L)-absorbance of I crude oil, determines petroleum ether extract respective sample
Concentration, according to concentration calculate degradation efficiency.
The computational methods of oil degradation rate refer to below equation：
η (%)=(1-Ct/Co) × 100%
Wherein：η-oil degradation rate；Crude oil concentration in the presence of Ct- bacterial strains；Co- compares crude oil concentration.
Result of the test：
Calculate defect shortwave monad 1-8 according to above-mentioned computing formula is to all degradation rates of crude oil pollution water body
85%.
Defect shortwave monad (Brevundimonas diminuta) 1-8 is to II crude oil pollution water bodys for embodiment 4
Degraded
Test method：
(1) preparation of defect shortwave monad (Brevundimonas diminuta) 1-8 bacteria suspensions
With embodiment 3.
(2) drafting of II crude oil calibration curve
II crude oil picks up from Changqing oilfields, and density is 0.87g/mL.Take 0,1,1.5,2,2.5,3mgII crude oil is in 50mL
In colorimetric cylinder, with carbon tetrachloride graduation mark is settled to, after being serially diluted so that concentration be respectively 0,20,40,50,60,70mg/L,
Under its maximum absorption wavelength 288nm, with carbon tetrachloride as reference solution, the absorbance of bioassay standard concentration.Abscissa is dense
Degree (mg/L), ordinate is absorbance, and the calibration curve for drawing concentration (the mg/L)-absorbance of II crude oil is as shown in Figure 5.
(3) measure of oil degradation rate
Specific assay method is with embodiment 3.
Result of the test：The defect shortwave monad 1-8 bacteria suspensions of 1.2mL and 4.2mL are calculated according to above-mentioned computing formula
5.13% and 26.94% is respectively to all degradation rates of II crude oil pollution water bodys.
The investment of embodiment 5 prepares defect shortwave monad (Brevundimonas diminuta) 1-8 solid fungicides
Sodium alginate (Sodium alginate, SA) is that one kind has future and extensive types of natural gel, and quilt
It is known as low price and there is nontoxicity to microorganism.Addition carrier material can strengthen mechanical strength, the tolerance of SA gels
And provide adsorption site for microorganism.With sodium alginate as embedded material, CaCl2Solution is crosslinking agent, and alpha-lactose is nutrition
Thing adsorbent.
Preparation method is followed the steps below：
1. the alpha-lactose of 4g sodium alginates, 1.5g biomass carbons, 0.5g is mixed, and adds 100mL distilled water slowly to stir
Mix, note activated carbon stirs outward during stirring, stirring amplitude is unsuitable excessive, prevents a large amount of bubbles from entering sodium alginate molten
Liquid, stirs autoclaving 20min under the conditions of the solution is placed in into 121 DEG C after terminating.Wherein, biomass carbon derives from Shaanxi beautiful jade base
Dali branch company of bioenergy Co., Ltd straw thermal cracking biomass fuel project is measured, physicochemical property is：Ammonium nitrogen
2.69ppm, nitrate nitrogen 4.34ppm；Nitrogen content 7.03ppm；Phosphorus content 12.54ppm；Potassium content 417.32ppm；The content of organic matter
3.96%；PH is 7.05.
2. after subject to sterilization, 30 DEG C or so are cooled to, add 20mL according to defect shortwave monad in embodiment 3
The defect shortwave monad 1-8 bacteria suspensions for activating 36h obtained by 1-8 bacteria suspension preparation methods, are mixed respectively uniformly,
Respectively obtain mixed liquor.
3. mixed liquor is clamp-oned into the CaCl that mass percent is 4% with 10mL disposable syringes2In solution, 24h is crosslinked,
Form fixed microballoon, the defect shortwave monad 1-8 solid fungicides that as prepared by investment.
4. filter, then with aseptic water washing three times, in being immersed in physiological saline, 4 DEG C save backup.
The absorption method of embodiment 6 prepares defect shortwave monad (Brevundimonas diminuta) 1-8 solid fungicides
With biological material and its correspondence charcoal as destination carrier, oil degradation Mixed Microbes are fixed by absorption method.
Fixing means is followed the steps below：
1. 1.5g biomass carbons are added in the 250mL conical flasks equipped with 100mL beef-protein mediums, and is added
20mL according to the defect shortwave monad 1-8 bacteria suspensions for activating 36h resulting in embodiment 3, mixing.
2. conical flask is placed under the conditions of 30 DEG C, rotating speed for 120r/min shaking table in, fix 18 hours.
3. then 10min is centrifuged under the conditions of 3000rpm, removes supernatant, lower sediment is cleaned into 2 with sterilized water water
It is secondary.
4. it is placed in culture dish and is dried in 30 DEG C of baking ovens, the defect shortwave monad 1-8 that as prepared by absorption method
Solid fungicide, in being immersed in physiological saline, 4 DEG C save backup.
Degraded of the defect shortwave monad 1-8 solid fungicides of embodiment 7 to II crude oil pollution water bodys
Defect shortwave monad 1-8 solid fungicides prepared by embodiment 5 and embodiment 6 are used for into degrading crude oil contaminant water
Body, it is specific as follows：Defect shortwave monad 1-8 solid fungicides prepared by 5g investments and absorption method are taken respectively, are respectively connected to 1mL
30 DEG C in II crude oil/100mL crude oil minimal mediums, after the incubated 7d of 120r/min, determine its removal to crude oil
Effect, and the degradation rate of II crude oil pollution water bodys is contrasted with defect shortwave monad 1-8 bacteria suspensions, as a result such as table 2
It is shown.
The degradation rate of the defect shortwave monad 1-8 solid fungicides of table 2 and bacteria suspension to II crude oil pollution water bodys
As shown in Table 2, the solid fungicide of defect shortwave monad 1-8 also there is degraded to repair II crude oil pollutions water body
Effect, it is compared with the degradation effect of bacteria suspension, as a result show the degradation effect of solid fungicide preferably, and adsorb legal system
Degradation effect of the degradation effect of standby solid fungicide better than solid fungicide prepared by investment.
Degraded of the defect shortwave monad 1-8 solid fungicides of embodiment 8 to II oil contaminated soils
Defect shortwave monad 1-8 solid fungicides prepared by embodiment 5 and embodiment 6 are used for into degrading crude oil Polluted Soil
Earth, it is specific as follows：
Crude oil pollution soil sample：The soil sample that 1mm sieves dry was weighed, was carried out by crude oil pollution degree and the quality for weighing soil sample
Weigh and stirred evenly in addition soil sample, crude oil pollution degree is 10g/kg, according to C：N：P is 100：10：1 ratio admixes (NH4)2SO4With
KH2PO4As N sources and P sources.Particularly, the nutriment of microorganism also includes C, but oil pollution result in the big of C sources
Amount increases, it is sufficient to ensure its supply, it is not necessary to additionally add.The physicochemical property of soil sample is：Ammonium nitrogen 5.75ppm, nitrate nitrogen
4.70ppm；Nitrogen content 10.45ppm；Phosphorus content 16.41ppm；Potassium content 76.45ppm；The content of organic matter 2.35%；PH is
7.27。
Test method：
Defect shortwave monad 1-8 solid fungicides prepared by 5g investments and 5g absorption methods are taken respectively, are respectively connected to aqueous
Rate is controlled in the soil of the 100g crude oil II pollution concentrations 1% of 12%-15%, 120r/min, 30 DEG C of incubated 7d,
Its removal effect to crude oil II is determined after 14d, 21d, its degradation rate is drawn.
Soil petrochina hydrocarbon content determination method, is carried out according to the following steps：
1. weigh 5g and air-dry soil sample, in being put into 50mL plastic centrifuge tubes.
2. 10mL carbon tetrachloride (analysis is pure), ultrasonic extraction 1h (temperature is less than 30 DEG C), leaching liquor are added with pipette
During 25mL volumetric flasks are filled into Jing after anhydrous sodium sulfate drying, continuation 2 × 10mL carbon tetrachloride hot dipping two times, each 20min,
Filtrate is incorporated to after drying volumetric flask, uses carbon tetrachloride constant volume.
3., in 288nm wavelength, with carbon tetrachloride as the absorbance of reference determination sample, reference standard is bent for spectrophotometer
The anti-concentration for pushing away crude oil of line.
Defect shortwave monad 1-8 solid fungicides are as shown in table 3 to the Degrading experiment result of oil contaminated soil.
Degradation rate of the defect shortwave monad 1-8 solid fungicides of table 3 to oil contaminated soil
| Bacterial preparation process | 7d degradation rates/% | 14d degradation rates/% | 21d degradation rates/% |
| Investment | 12.59 | 14.41 | 17.50 |
| Absorption method | 10.06 | 12.98 | 29.11 |
As shown in Table 3, the defect shortwave monad 1-8 solid fungicides that prepared by investment and absorption method are to oil contaminated soil
Degraded repairing effect is respectively provided with, and the solid fungicide degradation effect that within the degraded short time prepared by investment is preferably, and when degraded
When time reaches 21 days, solid fungicide prepared by absorption method is obviously improved to the degradation rate of oil contaminated soil, degradation effect
Substantially.
Although with a general description of the specific embodiments the present invention is described in detail in this specification,
But on the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.
Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, belong to claimed model
Enclose.
SEQUENCE LISTING
<110>Chang An University
<120>A kind of defect shortwave monad, microbial inoculum and its application
<130> 2016
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 1206
<212> DNA
<213>Defect shortwave monad（Brevundimonas diminuta）1-8
<400> 1
GGTGAGTAAC ACGTGGGAAC GTGCCTTTAG GTTCGGAATA GCTCCTGGAA ACGGGTGGTA 60
ATGCCGAATG TGCCCTTCGG GGGAAAGATT TATCGCCTTT AGAGCGGCCC GCGTCTGATT 120
AGCTAGTTGG TGAGGTAACG GCTCACCAAG GCGACGATCA GTAGCTGGTC TGAGAGGATG 180
ACCAGCCACA CTGGGACTGA GACACGGCCC AGACTCCTAC GGGAGGCAGC AGTGGGGAAT 240
CTTGCGCAAT GGGCGAAAGC CTGACGCAGC CATGCCGCGT GAATGATGAA GGTCTTAGGA 300
TTGTAAAATT CTTTCACCGG GGACGATAAT GACGGTACCC GGAGAAGAAG CCCCGGCTAA 360
CTTCGTGCCA GCAGCCGCGG TAATACGAAG GGGGCTAGCG TTGCTCGGAA TTACTGGGCG 420
TAAAGGGCGC GTAGGCGGAT CGTTAAGTCA GAGGTGAAAT CCCAGGGCTC AACCCTGGAA 480
CTGCCTTTGA TACTGGCGAT CTTGAGTATG AGAGAGGTAT GTGGAACTCC GAGTGTAGAG 540
GTGAAATTCG TAGATATTCG GAAGAACACC AGTGGCGAAG GCGACATACT GGCTCATTAC 600
TGACGCTGAG GCGCGAAAGC GTGGGGAGCA AACAGGATTA GATACCCTGG TAGTCCACGC 660
CGTAAACGAT GATTGCTAGT TGTCGGGCTG CATGCAGTTC GGTGACGCAG CTAACGCATT 720
AAGCAATCCG CCTGGGGAGT ACGGTCGCAA GATTAAAACT CAAAGGAATT GACGGGGGCC 780
CGCACAAGCG GTGGAGCATG TGGTTTAATT CGAAGCAACG CGCAGAACCT TACCACCTTT 840
TGACATGCCT GGACCGCCAC GGAGACGTGG CTTTCCCTTC GGGGACTAGG ACACAGGTGC 900
TGCATGGCTG TCGTCAGCTC GTGTCGTGAG ATGTTGGGTT AAGTCCCGCA ACGAGCGCAA 960
CCCTCGCCAT TAGTTGCCAT CATTTAGTTG GGAACTCTAA TGGGACTGCC GGTGCTAAGC 1020
CGGAGGAAGG TGGGGACGAC GTCAAGTCCT CATGGCCCTT ACAGGGTGGG CTACACACGT 1080
GCTACAATGG CAACTACAGA GGGTTAATCC TTAAAAGTTG TCTCAGTTCG GATTGTCCTC 1140
TGCAACTCGA GGGCATGAAG TGGGAATCGC TAGTAATCGC GGATCCGCAT GCCGCGGTGA 1200
ATACGT 1206
Claims (5)
1. a kind of defect shortwave monad, it is characterised in that be preserved in Chinese microorganism strain preservation on 09 12nd, 2016
Administration committee's common micro-organisms center, preserving number is CGMCC No.13002；It is named as defect shortwave monad
(Brevundimonas diminuta)1-8。
2. defect shortwave monad according to claim 1, it is characterised in that the defect shortwave monad
The 16S rDNA sequences of (Brevundimonas diminuta) 1-8 are as shown in SEQ ID No.1.
3. a kind of defect shortwave unit cell bacteria agent, it is characterised in that the microbial inoculum is solid fungicide, and with defect shortwave monad
(Brevundimonas diminuta) 1-8 is active component.
4. application of the defect shortwave unit cell bacteria agent described in claim 3 in degrading crude oil polluted-water.
5. application of the defect shortwave unit cell bacteria agent described in claim 3 in degrading crude oil contaminated soil.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611244659.7A CN106635910B (en) | 2016-12-29 | 2016-12-29 | Brevundimonas defective, microbial inoculum and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611244659.7A CN106635910B (en) | 2016-12-29 | 2016-12-29 | Brevundimonas defective, microbial inoculum and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106635910A true CN106635910A (en) | 2017-05-10 |
| CN106635910B CN106635910B (en) | 2023-06-23 |
Family
ID=58835639
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611244659.7A Active CN106635910B (en) | 2016-12-29 | 2016-12-29 | Brevundimonas defective, microbial inoculum and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106635910B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107502366A (en) * | 2017-09-05 | 2017-12-22 | 杭州更蓝生物科技有限公司 | A kind of complex microorganism preparations |
| CN108315283A (en) * | 2018-04-20 | 2018-07-24 | 史丹利化肥遂平有限公司 | A kind of disease-resistant growth-promoting type complex micro organism fungicide and its manufacturing method |
| CN110438033A (en) * | 2019-07-02 | 2019-11-12 | 浙江工业大学 | A kind of grease degrading strain, application and oils degradation method |
| CN112175865A (en) * | 2020-09-29 | 2021-01-05 | 中国农业科学院农产品加工研究所 | Brevundimonas diminuta, application thereof and method for degrading mycotoxin |
| CN114703081A (en) * | 2022-01-03 | 2022-07-05 | 昆明理工大学 | Brevundimonas ST3CS3 and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102888392A (en) * | 2012-10-11 | 2013-01-23 | 同济大学 | Preparation method of floating embedding bacterium agents for in-situ remediation of oil polluted water |
| US20140141494A1 (en) * | 2012-05-31 | 2014-05-22 | Uniwersytet Warszawski | Composition of bacterial strains, bioremediation mixture and use of this composition for the removal of contaminants from the soil and the method for purifying the soil contaminants |
| CN103881947A (en) * | 2014-02-26 | 2014-06-25 | 东北农业大学 | Brevendimonas diminuta strain for forming biofilm and application thereof in cyanide-containing wastewater treatment |
-
2016
- 2016-12-29 CN CN201611244659.7A patent/CN106635910B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140141494A1 (en) * | 2012-05-31 | 2014-05-22 | Uniwersytet Warszawski | Composition of bacterial strains, bioremediation mixture and use of this composition for the removal of contaminants from the soil and the method for purifying the soil contaminants |
| CN104583389A (en) * | 2012-05-31 | 2015-04-29 | 华沙大学 | Composition of bacterial strains, bioremediation mixture and use of this composition for the removal of contaminants from the soil and a method for purifying of the soil contaminants |
| CN102888392A (en) * | 2012-10-11 | 2013-01-23 | 同济大学 | Preparation method of floating embedding bacterium agents for in-situ remediation of oil polluted water |
| CN103881947A (en) * | 2014-02-26 | 2014-06-25 | 东北农业大学 | Brevendimonas diminuta strain for forming biofilm and application thereof in cyanide-containing wastewater treatment |
Non-Patent Citations (2)
| Title |
|---|
| XIN WANG ET AL.: "Bioremediation of marine oil pollution by Brevundimonas diminuta: effect of salinity and nutrients" * |
| 杨金凤;曹冠楠;杨正礼;: "砂箱中柴油降解菌株的分离鉴定" * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107502366A (en) * | 2017-09-05 | 2017-12-22 | 杭州更蓝生物科技有限公司 | A kind of complex microorganism preparations |
| CN108315283A (en) * | 2018-04-20 | 2018-07-24 | 史丹利化肥遂平有限公司 | A kind of disease-resistant growth-promoting type complex micro organism fungicide and its manufacturing method |
| CN108315283B (en) * | 2018-04-20 | 2021-02-26 | 史丹利化肥遂平有限公司 | Disease-resistant growth-promoting compound microbial agent and preparation method thereof |
| CN110438033A (en) * | 2019-07-02 | 2019-11-12 | 浙江工业大学 | A kind of grease degrading strain, application and oils degradation method |
| CN110438033B (en) * | 2019-07-02 | 2021-04-27 | 浙江工业大学 | Grease degrading bacterium, application and grease degrading method |
| CN112175865A (en) * | 2020-09-29 | 2021-01-05 | 中国农业科学院农产品加工研究所 | Brevundimonas diminuta, application thereof and method for degrading mycotoxin |
| CN112175865B (en) * | 2020-09-29 | 2022-04-08 | 中国农业科学院农产品加工研究所 | Brevundimonas diminuta, application thereof and method for degrading mycotoxin |
| CN114703081A (en) * | 2022-01-03 | 2022-07-05 | 昆明理工大学 | Brevundimonas ST3CS3 and application thereof |
| CN114703081B (en) * | 2022-01-03 | 2023-04-28 | 昆明理工大学 | Brevundimonas ST3CS3 and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106635910B (en) | 2023-06-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106635910A (en) | Brevendimonas diminuta, bacterial agent and applications of microbial agent | |
| US9404163B2 (en) | Pseudomonas putida strain as well as its microbial inoculum and application | |
| CN106635908A (en) | Advenella kashmirensis, microbial agent and application of advenella kashmirensis and microbial agent | |
| CN106635909A (en) | Crude oil degradation mixed bacterium, microbial agent and application of microbial agent | |
| CN106834269A (en) | A kind of immobilized microspheres of PAEs degradation bacterias and its preparation method and application | |
| CN114107092B (en) | Endophyte Gordonia L191 for degrading phthalate and application thereof | |
| CN106967641A (en) | A kind of micro- acidophilus Stenotrophomonas and its application | |
| CN118028186B (en) | Salt-tolerant enterobacter cholerae Hb, microbial agent and application thereof | |
| CN115786179A (en) | Bacterial strain for degrading o-dichlorobenzene and application thereof | |
| CN101811779B (en) | Preparation method of halophilic decontamination bacterial agent and bacterial agent prepared by same | |
| CN105018392A (en) | Long-chain alkane degrading bacterium and application thereof | |
| CN108676763B (en) | High-antimony-resistance proteus cassiicola DSHN0704 and separation and screening method and application thereof | |
| CN109971691A (en) | One plant of selenium-rich bacterium and its separation method | |
| CN108085313A (en) | A kind of method of microalgae semi-solid preparation mixed breeding processing fracturing outlet liquid | |
| CN105112345A (en) | Sphingobium sp. IBY and application thereof in adsorbing and degrading hydrophobic organics | |
| CN104694435B (en) | One plant of Shinella sp. with triazole degradation function and its application | |
| CN106801025A (en) | One plant of oil-base mud well drilling detritus degradation function bacterium and its application | |
| CN104745515B (en) | A kind of acinetobacter calcoaceticus of degrading polycyclic aromatic hydrocarbons and its application | |
| CN109097309B (en) | Petroleum hydrocarbon degrading bacteria and fermentation method thereof | |
| CN106754528B (en) | Pseudomonas flexi and application thereof | |
| CN106479942B (en) | A kind of bacillus thuringiensis and its application | |
| CN102533595B (en) | Starkeya sp. T-2 and application thereof | |
| CN107760636A (en) | Using low-quality carbon source phenol as the denitrification bacterial strain of electron donor and its application | |
| CN107446822A (en) | A kind of cultural method of rich oil scenedesmus obliquus | |
| CN110818180A (en) | Method for digesting black and odorous river channel by using microbial preparation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20230703 Address after: 518000 1002, Building A, Zhiyun Industrial Park, No. 13, Huaxing Road, Henglang Community, Longhua District, Shenzhen, Guangdong Province Patentee after: Shenzhen Wanzhida Technology Co.,Ltd. Address before: 710064 No. 33, South Second Ring Road, Shaanxi, Xi'an Patentee before: CHANG'AN University |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20240424 Address after: 710000, Room 1902, Building A, Jiahe Business Building, Cuihua South Road, Qujiang New District, Xi'an City, Shaanxi Province Patentee after: Xi'an Lu'an Environmental Protection Technology Co.,Ltd. Country or region after: China Address before: 518000 1002, Building A, Zhiyun Industrial Park, No. 13, Huaxing Road, Henglang Community, Longhua District, Shenzhen, Guangdong Province Patentee before: Shenzhen Wanzhida Technology Co.,Ltd. Country or region before: China |