KR101799780B1 - Method for Manufacturing Plant Nutrient Containing Mineral Element - Google Patents

Abstract

The present invention relates to a method for producing a plant nutrient that enables plants to utilize these components in a balanced manner while maintaining the essential minerals necessary for plant growth.
The plant nutrient produced by the method according to the present invention has a balanced balance of inorganic substances essential for crop growth and can be utilized efficiently by the crops. Therefore, the plant nutrition has excellent growth regardless of the kind of the crop, and various methods such as rooting, Can be widely used. It is not harmed by insect pests even if it does not spray pesticides due to antibacterial, antiviral and insecticidal effects. Also, it has a relatively low manufacturing cost and excellent storability, It can be applied to the livestock field as a livestock feed, and it can be used as a health food by applying it to human body.

Description

TECHNICAL FIELD The present invention relates to a method for producing a plant nutrient containing a mineral component,

The present invention relates to a method for producing a plant nutrient that enables plants to utilize these components in a balanced manner while maintaining the essential minerals necessary for plant growth.

The use of chemical fertilizers and pesticides has been rapidly expanding due to population growth and industrial development. The practice of agricultural chemicals, which are concentrated on chemical fertilizers and pesticides, has resulted in lowering of intellect and soil acidification, have.

In general, soil fertilization is for a large number of crops, and the increase in yield is expected to be somewhat expected. However, the use of a large amount of fertilizer increases the production cost of agricultural products, disadvantageous in terms of fertilizer efficiency and environmental preservation, The overuse of pesticides to remove them has increased resistance of pathogens and harmful nematodes in the soil, and the vicious cycle of using pesticides again has been repeated.

In general, fertilizer can be classified into inorganic fertilizer (chemical fertilizer) obtained by chemical treatment of inorganic raw material and organic fertilizer made of the same vegetable raw material, among which inorganic fertilizer includes nitrogen (N), phosphorus (P), potassium (K) It contains minerals such as magnesium (Mg), lime (Ca) and boron (B). It is mass produced by the chemical industry and therefore it is uniform in quality, stable in supply, low in price, hygienic compared to organic fertilizer Has been widely used in most farm households because of its simple and easy to use, high and constant contents of nitrogen and phosphorus, low cost of transportation, easy storage and fertilizing suitable for its purpose .

However, excessive use of inorganic fertilizers acidifies soil and reduces organic fertilizer to lower soil productivity. In the long run, it not only damages crops but also destroys the aggregation of the soil, And deterioration of water retention, which in turn causes poor growth of plants.

In order to solve the above problems, an agricultural method using an organic fertilizer instead of a chemical fertilizer has been developed and interest in fermentation compost using microorganisms is increasing.

Most of these organic fertilizers utilize sawdust, herb medicine waste, fish meal or animal food and natural organic materials mixed with them. In order to produce organic fertilizer by fermenting them, time, manpower and equipment required for microbial fermentation are required, And the generation of wastewater due to the production of such organic fertilizer causes water pollution.

In addition, if a fertilizer containing such an organic matter is used for a long period of time, it may damage the growth of crops, and organic acids may be formed in the process of corruption of livestock or fish meal, thereby damaging crop roots.

In addition, overuse of animal composts is likely to occur due to the accumulation of salts, resulting in impairment of calcium absorption due to excessive ammonia, and these organic fertilizers and chemical fertilizers are deficient in trace elements required for crop growth, Weaknesses have disadvantages as plant growth aids.

In addition, plants are limited by the minimum amount of nutrients that can be used on the basis of the required amount of plant by the Liebig's law of minimum.

Therefore, in the case of insufficient minerals such as nitrogen, phosphorus, and potassium in the soil, if the organic fertilizer containing a large amount of organic fertilizer is supplied, only the acidification of the soil is promoted, In order to grow, it is necessary to continuously inject minerals in addition to organic components.

Currently, researches on nutrients and fertilizers that can effectively provide these minerals in the agricultural and horticultural fields have been widely carried out. Mineral components for supplying the plants are mainly composed of silicates such as silicates, water-solubility.

However, the method of preparing various mixed minerals required for plants by mixing such single salts is difficult to manufacture due to the action of coagulation, sedimentation, etc., and a method of preparing mixed minerals by mixing chemically prepared single salts is a method It is difficult to prepare various minerals necessary for the plant, and when chemically produced mineral components accumulate in the soil, some of the components may be concentrated in the soil and have an undesirable influence on the plant.

In addition, the plant nutrient for leaf surface application has a relatively fast effect, and unlike the soil fertilizer, the amount of unnecessary consumption can be minimized so that the absorption rate is excellent. However, most of the plant nutrients for leaf surface application are only used for leaf surface application The use of the plant is limited, and it is inconvenient to use. Therefore, it often occurs that the plant is over-induced by excessive amount of trace elements. Therefore, it does not cause problems such as acidification of the soil and reduction of organic content, A plant nutrient that can be safely used throughout the entire process is required.

Accordingly, Korean Patent Registration No. 0976596 proposes an invention relating to an environmentally friendly plant nutrient that can be safely used throughout the entire growth period of a plant from germination to harvest, and is characterized in that a lysine fermentation by-product by the desalting and concentration of condensed molasses solubles (CMS) A water-soluble complex amino acid powder obtained by hydrolysis of keratin, desalted and freeze-dried, an aqueous solution of chitosan in which 10% by weight of chitosan powder was dissolved in an aqueous 3-5% acetic acid solution, , And one or more kinds of vitreous rhyolite powders selected from the group consisting of sandstone, obovate, and obsidian are heated and filtered at 70 ° C for 1 to 2 days in the same weight ratio water, and then mixed, filtered, heated, Plant nutrients are produced.

The present invention has enabled the high-quality growth and large-scale cultivation of plants by using the respective effects of lysine fermentation by-product liquid, water-soluble complex amino acid powder, chitosan aqueous solution and vitreous rhyolite powder. However, when the plant nutrients are diluted with water The plant nutrients of the present invention can be used throughout the entire growth period of the plant. However, the plant nutrients of the present invention can be used throughout the whole plant growth, It does not show all the benefits of nutrients in the process.

Korean Patent Registration No. 0979931 discloses a method of mixing minced ingredients (vermiculite, kelp, seaweed, chitosan, wood vinegar, leek, and extracted with sulfuric acid) and water in pulverized charcoal and maintaining the temperature at 40 to 80 ° C A method of producing a liquid fertilizer capable of promoting the growth of plants while being natural-friendly through a method of agitating the mixture is proposed.

The above invention also tried to collect all the ingredients needed for plant growth by collecting the potency of each component. However, because the extraction of the mineral component is made by sulfuric acid, the strong acidic sulfuric acid kills the useful microorganisms of the soil and further acidifies the soil In addition, if sulfuric acid remains in the plant and it is ingested without thoroughly washing or removing it, it can cause very serious health consequences.

In addition, Korean Patent Laid-Open Publication No. 2006-0059631 proposes a method for producing a plant nutrient of a natural extract that allows a plant to grow smoothly during the flower stage of a plant. + Kelp, carrots + bamboo shoots and spinach are mixed with brown sugar, loess, and charcoal powder, fermented for 30 to 50 days, mixed with brown rice vinegar and wood vinegar, and aged at low temperature for 3 to 4 years. After fermentation for 30 ~ 50 days, the plant nutrient is prepared by the process of aging at low temperature for 1 ~ 2 years.

The above-mentioned invention has an effect of not only fertilization effect of plant differentiation, flowering and fertilization but also effect on growth of plants and sugar content of fruit upon foliar application and effect of soil improvement by microorganism propagation effect in soil when traveling , Because the main material is fruit or seaweed, it takes a lot of materials and the manufacturing period is about 4-6 years, which is problematic in terms of economy and practicality.

Therefore, it is required to develop a method for producing a plant nutrient which can be practically used by the farmer in consideration of economical efficiency and ease of use, while improving the cultivation soil while increasing the crop yield.

It is an object of the present invention to solve the above-mentioned problems, and it is an object of the present invention to provide a process for producing a plant which is economical and easy to use, exhibits nutritional supply and sterilization effect throughout the whole process of growing crops, And to provide a method for producing a plant nutrient which is excellent and can prevent pests and diseases.

In order to solve the above-mentioned problems, the present invention provides a method for producing a microorganism, which comprises 0.5 to 7.0 parts by weight of a seaweed powder, 0.5-1.0 parts by weight of green tea powder, 5-10 parts by weight of bamboo powder, 5-10 parts by weight of rice bran powder, 5 parts by weight and 1 to 5 parts by weight of a saccharide are mixed to prepare a mixture; Lactic acid bacteria and yeast are inoculated into the mixture and anaerobically fermented at 27 to 35 DEG C for 10 to 20 days; And a step of inoculating the anaerobically fermented mixture with Bacillus subtilis and fermenting aerobically for 3 to 5 days at 29 to 33 ° C. The present invention also provides a method for producing a plant nutrient containing a mineral component.

At this time, it is preferable that the seaweed powder is prepared by mixing 80-120 parts by weight of the fruit juice obtained by juice-free roots in 100 parts by weight of seaweed powder, and aging the mixture at 25-35 ° C for 10-15 hours.

The minerals may be at least one selected from the group consisting of silicon, iron, calcium, magnesium, potassium, sodium, copper, sulfur, phosphorus, manganese, zinc, boron, molybdenum and chlorine.

Preferably, the lactic acid bacteria are at least one selected from the group consisting of Lactobacillus casei, Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus lactis, and the yeast is Saccharomyces cerevisiae.

Further, it is preferable to add 1 to 8 parts by weight of chitosan powder based on 100 parts by weight of water to the mixture, and more preferably the chitosan is a low molecular weight water-soluble chitosan having a molecular weight of 5 kDa or less and a deacetylation degree of 85% or more.

Also, it is preferable to add 0.5 to 5.0 parts by weight of the ginseng culture root to 100 parts by weight of water to the mixture.

The plant nutrient produced by the method according to the present invention has a balanced balance of inorganic substances essential for crop growth and can be used efficiently by the crops. Therefore, regardless of the kind of the crops, the plant nutrition has excellent growth, and various methods such as rooting, , And it is not harmed by insect pests even if it does not spray pesticides because of antibacterial, antiviral and insecticidal effects.

In addition, it can be used for storage for a long time after manufacture with relatively low cost of manufacture and excellent storability. It is harmless to livestock and human body and can be applied to livestock field as livestock feed. Do.

The method for producing a plant nutrient according to the present invention comprises mixing seaweed powder, green tea powder, bamboo powder, rice bran powder, mineral mixture, and sugar in water, anaerobically fermenting the mixture with lactic acid bacteria and yeast, .

Seaweeds are a large-scale fishery resource that accounts for about 25% of total aquatic production, and have been widely used as raw materials for edible, medicinal, feed or food industries. Recently, It is becoming active.

The major feature of seaweeds is that they are rich in vitamins and minerals. Among them, vitamins are abundant in B1, B2, C, and niacin, and the minerals are especially abundant in brown algae. Etc. become a good source of minerals.

Seaweeds also contain a variety of nutrients such as taurine, n-3 fatty acid, alginic acid, fucoidan, laminarin, pectin, and dietary fiber, Functional ingredients are included.

Seaweeds having a high content of minerals such as sea tangle and seaweed can be selected as seaweeds. In seawater, dozens of trace elements such as calcium, potassium, magnesium, iron, iodine and phosphorus and various enzymes are dissolved. It decomposes plankton and carcasses in seawater to produce organic matter, and this organic becomes nutrient that grows seaweed.

In addition to various pigments such as carotene, xanthophyll and chlorophyll, kelp contains many carbohydrates such as mannitol, laminarin and alginic acid, which are components of cell wall, which are made by carbon assimilation. In addition, iodine, vitamin B2 Glutamic acid and other amino acids are contained. In particular, iodine, potassium and calcium are contained in many inorganic salts, so kelp is a good material for supplying inorganic salts.

Seaweed is a seaweed belonging to brown algae and is an alkaline material rich in minerals such as potassium, calcium, magnesium, strontium, phosphorus, iodine, sulfur, dietary fiber, linolic acid, .

Fucoidan contained in seaweed and sea tangle is a sulfated polysaccharide with a sticky structure. It is a combination of base sugar and sulfate group called fucose. It has anti-coagulant effect, antitumor action, antitumor action, antimicrobial action, It has been known that it has an antihypertensive action, antihypertensive action, inhibition of hypertension, induction of production of hepatocyte growth factor (HGF), inhibition of hyperglycemia, immune cell regulatory action, antiallergic action, antiviral action and is known as a basic raw material, dietary supplement and additive for functional foods.

In addition, alginic acid of brown algae is a mucopolysaccharide that constitutes a cell membrane of brown algae and is highly used as an industrial stabilizer, an emulsifying agent, a thickener, and a flocculant in water suspension because of its high viscosity.

However, coastal waters inhabited by seaweeds are susceptible to heavy metal contamination. Most of the pollutants are generated from river or land inflows and marine dumping of trash. In recent years, pollution from domestic sewage and coastal industrial complexes in large cities There is an increasing tendency for the influx of materials.

These heavy metal components are likely to accumulate in seaweeds due to various mechanisms such as adsorption and ion exchange mechanisms by polysaccharides such as alginic acid and fucoidan contained in seaweed, and energy-related metabolism, and these seaweeds Ingestion of plants that have absorbed fertilizers or plant nutrients can cause heavy metals to accumulate in the body and lead to health-related diseases.

In addition, the viscous alginic acid and fucoidan are sticky, so that when the plant nutrient is stored, the other ingredients are agglomerated and settled, which may lower the storage stability of the plant nutrient.

Therefore, it is necessary to remove the heavy metals remaining in the seaweeds and lower the stickiness of the alginic acid to improve the storage stability of the plant nutrients according to the present invention. For this purpose, the seaweed powder is mixed with the ascorbic acid- It is preferable to aging the polysaccharide and the polysaccharide to reduce the toxicity by decomposing the heavy metals of the seaweed and decomposing the viscous polysaccharide into small molecules from the polymer to lower the viscosity so that the components of the plant nutrients are not aggregated and settled, .

It is an annual or biennial herbaceous plant of the dicotyledonous plant. It contains glucose, sucrose, sugar of fructose, various kinds of organic acid and amino acid, diastase which decomposes starch to promote digestion and absorption, Enzymes such as amylase which is a starch degrading enzyme, and ascorbic acid.

In one room, uncooked juice has the effect of promoting digestion and poisoning, and it is said that beneficial for the five days, the body is lightened while the body is lightened, and the juice removes the wall, stops coughing, reigns blood, warms the heart, And drinking juice to control diarrhea.

In order to purify the toxicity of heavy metals in seaweeds using radish roots having the above-mentioned effects, firstly, the roots of the radishes which have been removed from the leaves are thoroughly washed and then the water is removed.

After removing the water-removed radish root, the jujube is extracted, and the juice is mixed with the seaweed powder and aged by thoroughly mixing. During the fermentation, the enzymes of the heavy metals and the free sugars contained in the seaweeds actively react with each other, Purify.

The mixing ratio of the seaweed powder and the jujube is not particularly limited, but it may be suitably adjusted based on the weight of the seaweed powder: no juice = 100: 80-120, and the aging may be carried out at 25-35 ° C for 10-15 hours.

During the aging, the ascorbic acid contained in the juice extract decomposes the oligosaccharide alginic acid and fucoidan into low molecular weight by acid hydrolysis, and the low-molecular polysaccharide has a lower viscosity than the high-molecular polysaccharide, thereby reducing the stickiness. Therefore, · Because the precipitation phenomenon is suppressed, the storage stability of the plant nutrient is improved.

Salicornia herbacea ( Salicornia herbacea ) is a salt-tolerant salt plant native to the seashore. It is also called a tungsten mud, because the stem of the stem is protruding. The saliva plant is richer and denser than the inland plant due to the soil component influenced by seawater. It contains a variety of minerals and enzymes that are contained in seawater while growing on land.

In addition, it is known that, in order to survive by adapting to the stress environment of salt, the greenhouse filters out harmful components such as chlorine, arsenic and mercury in the seawater through photosynthesis and concentrates good components such as sodium, calcium, potassium, magnesium and iron .

Bamboo is known to contain a large amount of minerals such as sodium, potassium, calcium, magnesium, iron, manganese, phosphorus, zinc, silicon and sulfur in a well balanced manner and exhibits excellent antioxidative and antioxidant properties, , Which is effective in antimicrobial and antioxidative activity of silicate or terpene components contained in bamboo, and terpene is particularly effective as an insecticide as a main component of phytoncide.

In addition, bamboo is a porous body with small cubes consisting of fine particles. It absorbs pesticides and heavy metals, and grows crops healthily faster. It maintains the freshness of fruits and vegetables through preservative effect, and as soil fertilizer and soil conditioner Effect.

Rice bran is also called rice bran. It is a crumbly mixture of peel, seed coat, hornblende and embryo which is obtained when rice is cultivated to make rice grains. It contains protein, dietary fiber, essential fatty acid, essential amino acid, vitamin B, E and minerals .

Phenolic compounds in rice bran have a strong antioxidative effect. Ferulic acid, caffeic acid, syringic acid and quercetin, which are functional ingredients, It has several beneficial effects on the human body.

The minerals added to the present invention means essential nutrients which are required to relatively small amount for the growth, maintenance and reproduction of living organisms. The minerals include silicon, iron, calcium, magnesium, potassium, sodium, copper, sulfur, phosphorus, zinc, Molybdenum, chlorine, and combinations thereof.

Commercialized minerals can be classified into metallic elemental minerals, chelated minerals and colloidal minerals. Metallic minerals are extracted from minerals. They are about 1 ㎛ in size and have an electrical cation The absorption rate of the human body is about 8 ~ 12%. Chelate minerals are wrapped with amino acids, enzymes and proteins in order to absorb more metallic minerals. The absorption rate is 40 ~ 60%. Colloidal minerals are plants It is a vegetable minerals obtained by extracting minerals absorbed from the soil. It is about 1 nm in size and has an electrical anion at 98%.

Therefore, vegetable colloidal minerals having excellent water absorption as the above-mentioned minerals used in the present invention are preferable.

Silicon is a long-lasting supplements that slowly dissolves in the soil and replenishes the fertilizer components. It is soluble in the acid which comes from the weak acid in the soil or the root of the crop, Component.

Silicon components enter the plant to form a siliceous layer on the cell wall to strengthen the cells, thereby increasing the resistance to pests and diseases. By increasing the disease resistance, it increases bacterial and viral diseases as well as resistance to cold weather. By controlling the fertilizer effect, it prevents nitrogen excess and phosphoric acid absorption obstacle, maintains freshness of plant, increases storability, and increases sugar content of fruit.

Iron acts as a catalyst for chlorophyll production and plays a role of disassembling oxygen transport, enzymatic activator and hydrogen peroxide. When iron is deficient, chlorophyll is not synthesized and yellowing phenomenon occurs because the leaves do not maintain blue light.

Calcium adjusts the pH, neutralizes organic acids in the plant, participates in protein synthesis, and helps nitrogen fixation of root-borne bacteria. When calcium is deficient, leaves are white or necrotic in the case of hardwoods, while young leaves are somewhat larger It is small and the part of the leaf is twisted. In the case of conifer, the growth of the upper part is stopped, the end of the leaf is damaged, and it is easily affected by drought and cold.

Magnesium is involved in the production of chlorophyll, phosphorus absorption and migration, and is necessary for the synthesis of milk fat. In the case of deficient leaf, leaf becomes thin and is easily broken and leaves early. In fully matured leaf, And the fruit is smaller than normal. In the case of conifer, the end of the leaf turns yellow and sometimes it turns red.

Potassium helps to overcome poor environmental conditions by strengthening resistance to frost and stigma. It plays an important role in water absorption or respiration in the pore opening and closing of plants, promotes the movement of photosynthetic products, Since the concentration of potassium in the cytoplasmic cells is lowered, the pores are closed and the leaves are wilted, and the chlorophyll formation in the leaves is decreased. Thus, after the pale yellow pattern is formed, it becomes brown, the growth rate of the cambium is decreased in the stem, Ligninization, which is inhibited and makes the stem hard, slows down, causing the tissue to soften, causing the plant to fall and become vulnerable to insect pests.

Sodium is involved in cell expansion and osmotic pressure control as well as in cell growth. It is involved in photosynthesis in CAM (crassulacean acid metabolism) plants and C4 plants, and copper helps plants to synthesize energy.

Sulfur is mainly absorbed in the form of sulfur dioxide to promote the growth of plants. When sulfur is deficient, it becomes dark yellowish green in the case of hardwoods and smaller than the normal leaf size in some species. In the case of softwoods, There are also cases.

Phosphorus promotes cell division, thereby increasing the number of organs in the plant. Especially, it makes flowers and fruits run a lot. It plays a role of root development, starch production, chlorophyll function, photosynthesis, respiration, physiology, In the absence of phosphorus, leaf veins, petiole and leaf bottoms turn red or purple in the case of hardwoods, younger leaves become smaller in size than normal leaves and early leaf fall occurs. In the case of conifers, .

Other manganese, zinc, boron, molybdenum, chlorine, etc. are involved in the growth and maturation of plants and play a role of coenzyme. If they are deficient, they will interfere with biochemical synthesis and decrease the nutritional value of the plant itself .

As described above, essential trace elements of minerals necessary for plant growth are contained in seaweed powder, green tea powder, bamboo powder, rice bran powder, and minerals, so that they are combined with each other to contain trace elements required in plant growth at an appropriate ratio, 0.5 to 7.0 parts by weight of seaweed powder, 0.5 to 1.0 part by weight of green tea powder, 5 to 10 parts by weight of bamboo powder, 5 to 10 parts by weight of rice bran powder, and 1 to 5 parts by weight of mineral are mixed with 100 parts by weight of water.

The saccharide acts as food for lactic acid bacteria and yeast microorganisms. It is economically advantageous to use molasses produced as a by-product in the production of sugar as the sugar. Unlike sugar, minerals are contained in molasses And is made up of polysaccharides (such as fibers), so that microorganisms use polysaccharides slowly.

Therefore, sugar is suitable for short-time fermentation because microorganisms can be used easily and quickly, and molasses is suitable for long-term fermentation. Therefore, in the present invention, molasses is slowly fermented for a long period by using molasses, and minerals contained in molasses are included in plant nutrients .

The amount of sugar is preferably 1 to 5 parts by weight per 100 parts by weight of water. If the content of sugar is less than 1 part by weight, the microorganism is insufficient in fermentation. If the amount is more than 5 parts by weight, the remaining amount remains after fermentation, Nutrients can be corrupted.

The microorganisms lactobacillus and yeast are anaerobically fermented with a mixture of seaweed powder, green tea powder, bamboo powder, rice bran powder, and minerals with sugar as a nutrient. By fermentation of lactic acid bacteria and yeast by lactic acid fermentation and alcohol fermentation, Is decomposed and lactic acid and alcohol are generated, and minerals contained in seaweed, green tea, bamboo and rice bran are separated and eluted into water.

The lactic acid bacteria may be any lactic acid bacteria that can be used for conventional fermentation of lactic acid bacteria, and preferably lactobacillus casei , lactobacillus acidophilus , Lactobacillus brevis , One or more of Lactobacillus lactis may be used, fermenting the saccharides to obtain energy and produce lactic acid.

The yeast is the MRS celebrity busy with a saccharide to fermentation at room temperature in MRS (Saccharomyces), and Saccharomyces strains can be used (Saccharomyces cerevisiae strain is preferably used, and the sugar is fermented and separated into alcohol and carbon dioxide gas.

Lactic acid produced by fermentation of lactic acid bacteria converts the fermentation product into an acidic atmosphere and inhibits the activity of germs and bacteria present in seaweeds, green tea, bamboo, and rice bran, activates yeast activities that favor a relatively acid environment , The growth and metabolism of yeast promotes the production of alcohol and carbon dioxide and the generation of heat.

In addition, lactic acid is fixed to the soil to dissolve the insoluble minerals so that they are absorbed into the plant roots and chelate with the fertilizer component to facilitate absorption of the plant, To prevent the occurrence of water edema in the rainy season.

Since the yeast is a mass of cells composed of organic nutrients, the soil is fertilized as the soil microorganisms act by using the organic nutrients of the yeast, and the plants absorb the nutrients, so that the growth is sound. Since the yeast also uses inorganic elements, So that the activity of the soil microorganism is increased and is easily absorbed into the root of the plant.

When anaerobically fermenting the seaweed powder, green tea powder, bamboo powder, rice bran powder, and mineral mixture with lactic acid bacteria and yeast at 27 to 35 ° C for 10 to 20 days, the organic matters of these mixtures are decomposed and lactic acid, alcohol, And lactic acid and alcohol increase the storage stability of the fermented product and the carbonic acid gas is evaporated and removed.

Chitosan powder may be further added to the mixture to further supplement the mineral component of the fermented product.

Chitosan is a cationic natural polymer that is abundantly present in the natural state after cellulose. The molecular structure of chitosan is very similar to that of cellulose, but it has various physicochemical properties due to presence of amino group (-NH ₂ ) in the molecule.

Chitosan can be obtained by deacetylation of chitin extracted from crustaceans of sea crabs or shrimp bark, mollusk bones, etc. The major component is polyglucosamine, which is a colorless amorphous powder with a polymer structure and has antibacterial, antifungal, It has an effect such as adsorption, physiological activity, human affinity, and wound healing promotion effect, and thus acts as an excellent natural antimicrobial agent.

Because of this effect, chitosan inhibits the growth of plant pests, inhibits the growth of fungi in the soil, promotes physiological activation, promotes the growth of the leaf surface and increases the disease resistance. Foliar spraying before and after flowering promotes fruiting, The storage stability of the vegetables can be improved.

In addition, it has an effect of discharging heavy metals and succulents in the human body or lowering the blood cholesterol, so that it is possible to prevent an adverse effect on the human body due to seaweeds contaminated with heavy metals.

Chitosan can be divided into polymeric chitosan, intermediate chitosan and low-molecular chitosan depending on the molecular weight. It is classified into water-soluble chitosan and water-insoluble chitosan depending on its solubility in water.

Therefore, the chitosan used in the present invention is preferably a low-molecular water-soluble chitosan dissolved in water, more preferably a molecular weight of 5 kDa (kilodalton) or less, a deacetylation degree of 85% or more, a molecular weight of 3-5 kDa, Or more.

The low molecular weight chitosan of the above molecular weight is mostly dissolved in water and the undissolved components are completely dissolved in the acidic condition by the lactic acid fermentation product lactic acid in the mixture to be absorbed by the plant and 1 to 8 parts by weight of chitosan powder is added to 100 parts by weight of water .

Also, in order to further enhance the leaf surface effect of the fermented product, the mixture may be further fermented by adding a wild ginseng culture muscle to the fermented product. Preferably, 0.5 to 5.0 parts by weight of the ginseng cultured root is added to 100 parts by weight of water.

The ginseng cultivar is a cultivar of ginseng cultivated in an artificial environment. Ginseng cultivar has the shape of a silkworm, unlike the actual ginseng, and it can be said that the specific saponin components of wild ginseng are cultivated to have the same genetic properties as wild ginseng without genetic modification.

Therefore, it retains the nutrition of natural wild ginseng. Especially, it contains saponin ingredient contained only in wild ginseng. It is beneficial not only to humans and animals but also to plants, and the plants sprayed with wild ginseng root components are resistant to pests and diseases And the growth vitality is enhanced, and it has a favorable influence on the sugar content, gloss, and size of the fruit.

That is, when the fermented product fermented with the wild ginseng cultured muscle is sprayed on the leaf surface, a coating is formed on the surface of the plant, the antifungal activity is enhanced to strengthen the self defense function, and the cuticula layer forming the epidermal cells of the plant is strengthened, Controls the growth, prevents surface drying of the plant, and promotes cell activity.

It also produces phenolic substances to prevent pathogens from entering, to produce immunity resistant to pathogens, to promote sugar and fat metabolism in plants, to promote protein synthesis, lipid synthesis, promote DNA synthesis in cells, Helps promote.

When fermenting the cultured ginseng root as described above, the saponin component of the ginseng cultured muscle is specifically converted into Rg1, Rb1, Rb2, Rc, Rd and the like by liquefaction by the microbes of lactic acid bacteria and yeast, To enhance the in vivo immunity and to inhibit harmful bacteria by obtaining the effect as probiotics.

The anaerobic fermented product produced as described above is rich in essential minerals necessary for plant growth, and thus can be used as a plant nutrient. It can be widely used for a variety of uses such as basal, smooth, and foliar application, and has antibacterial, Because of the effect, it does not harm the insect pests even if it does not spray the pesticide.

Bacillus subtilis is further fermented in order to further enhance the efficacy of the anaerobic fermentation product. Bacillus subtilis is an aerobic microorganism. Therefore, the fermentation with Bacillus subtilis is fermented at 29 to 33 ° C for 3 to 5 days while supplying air.

Bacillus subtilis decomposes organic matter, kills harmful bacteria, and produces a lipopeptide with antimicrobial activity, a surfactant that acts as an antibiotic substance, and a large amount of protein. It also secretes a useful enzyme such as amylase and protease It produces and promotes the formation of loess in the soil by releasing sticky decomposition enzyme.

Bacillus subtilis promotes the growth of plant roots and stems, promotes the growth of stem roots and endotoxins, and decomposes pathogens such as odors, fungi, and Escherichia coli to enhance plant tolerance, and potassium , Minerals such as phosphoric acid, calcium and the like, and supply them to plants.

In addition, the fermented Bacillus subtilis fungus increases the physiological strengthening of the crops and the leaf length and leaf width of the crop through the application of the foliage, and the active photosynthesis by the promoting of the leaf growth has a positive effect on the growth of the crops and increases the fruit yield, An enhancing effect can be obtained.

The plant nutrient prepared as described above is relatively inexpensive to manufacture, has a relatively short production period, and is sprayed on the soil or the leaf surface to exert a useful effect on plant growth, and is harmless to livestock and human body. And can be applied to the livestock field, and it can be applied to human body and used as health food.

Hereinafter, the present invention will be described in more detail with reference to the following examples, comparative examples and test examples.

It is to be understood, however, that the invention is not to be construed as being limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art. Will be apparent to those skilled in the art to which the present invention pertains.

≪ Example 1 >

10 kg of marrow powder, 10 kg of marine powder, 3 kg of green tea powder, 30 kg of bamboo powder, 30 kg of rice bran powder, silicon, iron, calcium, magnesium, potassium, sodium, copper, 7.5 kg of vegetable colloidal minerals (Orgmin, NUTRITION ™ USA, INC., USA) containing zinc, boron, molybdenum and chlorine and 7 kg of molasses were mixed to prepare a mixture.

The mixture was inoculated with a lactic acid bacterium (a mixed strain of Lactobacillus casei, Lactobacillus acidophilus, Lactobacillus brevis and Lactobacillus lactis) and yeast (Saccharomyces cerevisiae) and then sealed. The anaerobic fermentation product was again inoculated with Bacillus subtilis and aerated with air for 4 days at 31 ° C to produce a plant nutrient.

≪ Example 2 >

After removing the leaves, the roots of the radish were cleaned, and then the water was removed and the juice was extracted by pressing.

10 kg of seaweed powder and 10 kg of kelp powder were added to 20 kg of the above-mentioned juice, and the mixture was stirred sufficiently for 30 minutes at 30 ° C for 12 hours. , A plant nutrient was prepared in the same manner as in Example 1 above.

≪ Example 3 >

A plant nutrient was prepared in the same manner as in Example 1, except that 10 kg of low molecular weight water-soluble chitosan having an average molecular weight of 4 kDa and a degree of deacetylation of 98% or more was further added to the mixture of Example 1 above.

<Example 4>

A plant nutrient was prepared in the same manner as in Example 1, except that 10 kg of the cultured ginseng root was further added to the mixture of Example 1.

&Lt; Comparative Example 1 &

The same procedure as in Example 1 was carried out except that bamboo powder in the mixture of Example 1 was not mixed with water to prepare a mixture of only waxy powder, tangle powder, green tea powder, rice bran powder, mineral and molasses, Plant nutrients were prepared.

&Lt; Comparative Example 2 &

In Example 1, the anaerobic fermentation product was not fermented aerobically as Bacillus subtilis but was used as a plant nutrient as an anaerobic fermentation product.

<Test Example 1> Analysis of mineral content of fruit

The apples were cultivated in a manner such that the plant nutrients of Examples 1 to 4 and Comparative Examples 1 and 2 were further sprayed while the apples were grown in a conventional manner.

Soil 1 sugar in apple (hay) field in early March. 50 ㎏ of diluent (diluted 100 times by weight on water basis) diluted with plant nutrient prepared above was sprayed and mixed well with soil. From April to just before harvest The diluent was sprayed twice a month so that the fruit leaf surface was sufficiently wetted.

The apples cultivated in the conventional manner without fertilizing the plant nutrients were used as a control, and the minerals content of the apples harvested in the above Examples and Comparative Examples were measured and shown in Table 1 below.

For the measurement, 0.3 g of each of the edible parts was lyophilized, and the mixture was cooled at 600 ° C for 12 hours. After cooling sufficiently, 3 ml of 6 M hydrochloric acid was added, and the mixture was allowed to stand for 15 hours. Lt; / RTI &gt;

100 μl of the filtrate was quantitated to 3 ml with tertiary distilled water and then measured using an inductively coupled plasma mass spectrometer (ICP-MS, Model # 7500a, Agilent Technology, USA).

Mineral content analysis (unit: mg / 100 g) potassium calcium salt magnesium Example 1 13.8 1.9 5.7 3.0 Example 2 13.5 1.9 5.5 2.8 Example 3 14.1 2.1 5.5 3.2 Example 4 13.6 1.8 5.7 2.9 Comparative Example 1 12.2 1.6 5.2 2.5 Comparative Example 2 12.5 1.5 5.3 2.7 Control group 10.5 1.1 5.0 2.3

In Table 1, the mineral contents of the examples were increased compared to the control, and the mineral content of the Example 3 containing the low-molecular water-soluble chitosan was most excellent.

In Comparative Example 1, in which a plant nutrient was prepared without mixing minerals-rich bamboo powder such as sodium, potassium, calcium, magnesium and the like, the content of these minerals decreased. In Comparative Example 2, the plant nutrient was fermented And the effect of Bacillus subtilis, which is supplied to plants by dissolving the minerals fixed to the soil, is excluded.

&Lt; Test Example 2 > Quality measurement of fruit

The fruit weighed, the hardness, the sugar content and the acidity of the apple harvested in Test Example 1 were measured, and 10 samples were taken at random from each group, and their average values are shown in Table 2 below.

Quality measurement result Overweight (g) Hardness (kg / 5 mmΦ) Brix (° Bx) Acidity (%) Example 1 326 1.7 15.3 0.32 Example 2 327 1.5 16.0 0.30 Example 3 329 1.8 15.5 0.35 Example 4 331 1.7 15.6 0.30 Comparative Example 1 322 1.5 15.1 0.31 Comparative Example 2 318 1.4 14.8 0.33 Control group 315 1.3 14.5 0.31

In Table 2, the weight, hardness and sugar content of the apples sprayed with the plant nutrients of the examples and the comparative examples were increased as a whole compared to the control, and the results of the examples were higher than those of the comparative examples, .

The highest content of chitosan was determined in Example 4, and the saponin content of the cultured ginseng root increased the vitality of fruit juice. The hardness of chitosan was highest in Example 3, It is estimated that the sugar content contained is the result of increasing the sugar content of the fruit.

Unlike the case of Comparative Example 1, when bamboo powder containing minerals in a balanced manner is not mixed in the preparation of a plant nutrient, overage and sugar content are lowered due to imbalance of minerals, which are essential trace elements of plant growth. And the fruit weight, hardness and sugar content are all lowered.

&Lt; Test Example 3 > Measurement of quality of vegetables

The plant nutrients prepared in the above Examples and Comparative Examples were diluted 100 times and applied to cucumber (cucumber), and the weight and length of the harvested cucumber were compared to confirm the efficacy of the plant nutrient according to the present invention.

The plant nutrients of the above-mentioned examples and comparative examples were sprayed on the leaf surface twice a month in the growing season of the cucumber and harvested at the normal harvesting period, using the cucumber fertilized by the conventional method without fertilizing the plant nutrient of the present invention as a control group. The average values are shown in Table 3 below.

Quality measurement result Weight (g) Length (cm) Example 1 147 24 Example 2 144 23 Example 3 149 24 Example 4 150 25 Comparative Example 1 140 22 Comparative Example 2 138 22 Control group 132 21

As shown in Table 3, the cucumber of the Example showed excellent results in weight and length compared to the control, and Example 4 in which the plant germinability was enhanced by containing the ginseng root component was the most excellent.

The cucumbers of Comparative Examples 1 and 2 were superior to those of the control group, but were inferior to those of the control group, so that the plant growth promoting effect was better by mixing the bamboo powder and producing aerobic fermentation with Bacillus sp.

&Lt; Test Example 4 >

10 kg of the plant nutrients prepared in the above Examples and Comparative Examples were taken in each group, placed in a container, allowed to stand for 10 days, carefully weighed out the supernatant, weighed the sediment that had settled on the floor, poured the supernatant again Stirred for one minute so that it did not reach the sediment, weighed the supernatant and weighed the sediment that had settled on the bottom.

The measurement results are shown in Table 4 below, and the weight of the container was removed.

Sediment weighing results Weight of precipitate before agitation (g) The post-sediment weight (g) Example 1 203 43 Example 2 125 21 Example 3 273 55 Example 4 304 68 Comparative Example 1 229 58 Comparative Example 2 271 60

In Table 4, the precipitate of Example 2 was the least, because ascorbic acid, which is an organic acid of no-juice, degrades alginic acid and fucoidan, which are viscous polymers, into low-viscosity low-molecular acids by acid hydrolysis, It is judged that the agglomeration of the component particles is decreased. From the result of the increase of the precipitate in Example 4, it is presumed that the component of the ginseng culture root contained in the plant nutrient coagulates with the other components.

From the above results, it can be seen that it is preferable to add the fruit juice containing ascorbic acid, which is an organic acid component, to the plant nutrients in order to store the plant nutrition agent of the present invention containing the viscous polymer for a long period of time, The weight of the plant nutrient is very small compared to the weight of the whole plant nutrient, so that the plant nutrient of the present invention is stored for a long time even after storage for a while.

Claims (7)

0.5 to 7.0 parts by weight of seaweed powder, 0.5 to 1.0 part by weight of green tea powder, 5 to 10 parts by weight of bamboo powder, 5 to 10 parts by weight of rice bran powder, 1 to 5 parts by weight of minerals and 1 to 5 parts by weight of water, Preparing a mixture by mixing;
Lactic acid bacteria and yeast are inoculated into the mixture and anaerobically fermented at 27 to 35 DEG C for 10 to 20 days; And
Inoculating the anaerobically fermented mixture with Bacillus subtilis and fermenting aerobically for 3 to 5 days at 29 to 33 ° C. The method according to claim 1,
Wherein the seaweed powder is obtained by mixing 80-120 parts by weight of a fruit juice obtained by juicing radish roots into 100 parts by weight of a seaweed powder and aging the mixture at 25 to 35 ° C for 10 to 15 hours. A method for producing a plant nutrient. The method according to claim 1,
Wherein the mineral is at least one selected from the group consisting of silicon, iron, calcium, magnesium, potassium, sodium, copper, sulfur, phosphorus, manganese, zinc, boron, molybdenum and chlorine. A method for producing a plant nutrient. The method according to claim 1,
Wherein the lactic acid bacterium is at least one selected from the group consisting of Lactobacillus casei, Lactobacillus acidophilus, Lactobacillus brevis and Lactobacillus lactis, and the yeast is Saccharomyces cerevisiae. &Lt; / RTI &gt; The method according to claim 1,
A method for producing a plant nutrient containing a mineral component, which comprises adding 1 to 8 parts by weight of chitosan powder based on 100 parts by weight of water to the mixture. The method of claim 5,
Wherein the chitosan is a low-molecular water-soluble chitosan having a molecular weight of 5 kDa or less and a deacetylation degree of 85% or more. The method according to claim 1,
A method for producing a plant nutrient containing mineral components, which comprises adding 0.5 to 5.0 parts by weight of a ginseng cultivation root to 100 parts by weight of water.