KR20110024339A - Environmental friendly fermented liquid composition and method for preparing the same - Google Patents

Abstract

PURPOSE: A fermentation liquid composition containing fermented extract and ceramic catalyst is provided to improve soil and to recover fertility of soil. CONSTITUTION: A method for preparing fermentation liquid composition for agriculture comprises: a step of mixing Artemisia princeps var. orientalis, Houttuynia cordata, green tea extract, and saccharide in water and spraying the mixture to spawn culture medium for inoculation; a step of fermenting the spawn at 20-60°C for 6-7 hours to obtain a fermentation; a step of extracting the fermentation to obtain fermented extract; a step of adding nano-ceramic catalyst and additive to the fermented extract to form fermentation liquid composition; and a step of mixing the fermented extract, nano-ceramic catalyst, and additive in the ratio of 100:1-20:0.1-2.

Description

Environment friendly fermented liquid composition and method for preparing the same {Environmental friendly fermented liquid composition and method for preparing the same}

The present invention is a functional ceramic catalyst that can maximize the light utilization of fermentation broth and microorganisms and plants ripened and fermented green tea leaf extract (juice) containing a lot of wormwood or fish vinegar and catechins excellent in antibacterial properties can be collected in the field or on the market The present invention relates to a method for preparing a fermentation broth composition for environmentally friendly growth promoting a more diverse function and a continuous effect, and a fermentation broth composition prepared by such a method.

The damage caused by diseases or pests of plants has been the biggest obstacle to ensuring stable food for humans along with natural disasters. General statistics show that plant diseases or pest losses account for about 10% of the total production, which is much higher considering the degradation of pests produced. In addition, the occurrence of certain pests due to extreme weather or special environmental changes may cause serious social problems.

Moreover, the absolute shortage of sunshine due to the prolonged rainy season and cloudy days is thought to have a significant effect on not only the damage of pests but also the degradation of crops and the loss of yield. As part of such efforts to control diseases and pests, methods of developing artificially synthesized pesticides and controlling them have been mainly used in agriculture. However, the constant increase in population necessitates a greater amount of food, and the monoculture of the same high quality species leads to the mass production of pests and the abuse of synthetic pesticides for their control. Recently, problems such as the destruction of natural ecosystems, residual toxicity, toxicity to livestock, and the emergence of new pests that are drug-resistant, have been greatly caused. To replace these, new pesticides (low-toxic pesticides, natural pesticides or biological pesticides) have been developed. Research is underway, but the development speed is not meeting demand. In addition, the demand for rice farmers to produce high-quality rice without pesticides and chemical-free fertilizers is increasing as well-being, health food, environmental protection, and consumer's preference for high-quality rice. Increasing quality competitiveness and reducing farming costs are driving the demand for high-quality, uncontaminated, clean agricultural products without fear of increased intelligence and residual toxicity or destruction of natural ecosystems.

Recently, with the development of genetic engineering technology, disease-resistant transgenic plants have been proposed as an alternative to produce stable food crops with less pesticide use. Attempts are being made to develop new concepts of plant protection and nutrition that are not toxic to the environment.

However, the existing products are extracted or pelleted by simply fermenting the by-products to utilize the by-products to give compost or manure, as shown in Korean Patent Nos. 0481301, 0555077, and 061264. It could not escape the form of feeding fertilizer in the form of manure, which was processed or fertilized with microbial agents for the purpose of soil improvement.

Accordingly, an object of the present invention is to solve the social demands and existing problems as described above, for low-pesticides and chemical-free fertilizers, for the production of eco-friendly high-quality rice, to improve quality competitiveness and reduce farming costs for the production of clean agricultural products By combining the traditional aging and fermentation method of Korea with no problem of environmental pollution and photosynthetic microorganisms and nano ceramic catalysts, aging and fermentation of natural plant organic materials with high antimicrobial properties improves disease resistance of crops and functional ceramic with excellent hydrophilicity and adsorption power. The catalyst promotes photosynthesis and absorption of water for respiration, and maximizes the absorption of sunlight and utilization of light even in the case of a lack of sunshine due to the continuation of cloudy days. It is to provide a natural fermentation broth composition that can be.

Natural fermentation broth composition provided by the present invention contains a large amount of microorganisms, as well as organic matter and inorganic components to grow rice healthy to reduce the occurrence of pests and damage to fall, and also among the strongest antibacterial activity contained in the composition Fermented extracts of Eungseongcho, mugwort and green tea leaf extracts, which contain one or more antibacterial ingredients such as quercetin, quercitrin and catechin, as well as potassium, natural vitamins, minerals, iron, It contains abundant nutrients such as sodium, so it can be used simultaneously.

In general, fertilizers and nutrients are not easy because they are repeatedly applied in the form of salt or manure only for the simple effect of supplementing nutrients or insufficient N, P, K. However, the fermentation broth composition of the present invention can not absorb N, P, K, which are insufficient in the form of salts, or supply minerals or nutrients, but can absorb or light water, which is most important for the growth and photosynthesis of crops. By fertilizing the side, it is attached to the side or stem of the plant to increase the absorption of light and use of light, and the non-attached fermentation liquid composition falls on water or soil, and photosynthetic microorganisms multiply in the soil to supply oxygen and root uptake, Multipurpose natural fermentation broth that acts as a suppressor of harmful bacteria growth, and various minerals and nutrients are absorbed into the plant to increase the absorption effect of the crop and increase the physiological activity and anti-infection of the plant. It is a composition. Eco-friendly fermentation broth composition of the present invention is a super-hydrophilic and light absorption of nanoceramic, organic decomposition, microbial activity, various natural minerals and vitamins, antimicrobial substances, iron, sodium and other nutrients, such as plant stems, Mugwort useful for eco-friendly organic farming, which contributes to the reduction of labor and labor costs, production cost savings, and high-quality rice production by reducing the number of harvesting, pesticide spraying and dobok rice by promoting growth through not only roots but also soil activity growth of microorganisms. Method for producing a fermentation broth composition for eco-friendly fermentation liquid and a variety of effects, including fermentation broth and microorganisms and functional ceramic catalysts aged and fermented with green tea leaves or extracts containing a lot of catechins excellent in antibacterial activity It relates to a fermentation broth composition prepared by.

The above experiments show that the eco-friendly fermentation broth composition of the present invention facilitates the physiological function of plants, and the organic growth and the effect of promoting the growth of plants are excellent as the overall growth and prevention of pests are dramatically improved.

Therefore, the environment-friendly fermentation broth composition of the present invention is the antimicrobial activity of mugwort and eochocho, the nitrogen fixation of photosynthetic microorganisms and the action of nanoceramic catalyst that can absorb and use light, so that root development is smoothed, thereby helping root lubrication and consequently promoting growth and Help the fruit. In addition, it has excellent odor removal and removes odors such as ammonia, acetic acid, and lactic acid, which are the main causes of odors in pigs and poultry farms, and is used as an eco-friendly fermentation liquid with the effect of promoting egg production and growth. This is possible.

The natural fermentation broth composition of the patent contains a large amount of vegetable mugwort, fish vinegar, microorganism, as well as organic matter and inorganic ingredients, which makes rice grow healthy, reduce the incidence of pests and damages, reduce natural vitamins, iron, trace elements, etc. Use is effective.

It is useful for eco-friendly organic farming, which contributes to reducing the number of production and harvesting and pesticide spraying, reducing labor and labor costs, and reducing production costs by increasing the physiological activity and anti-infectious properties of plants and promoting growth.

The present invention is a method for producing a fermentation broth composition for agriculture, inoculated by spraying the spawn culture medium in a mixture of 20 to 70% by weight of mugwort, Echo and green tea leaf extract and 30 to 80% by weight of a sugar solution, Fermentation at 20 to 60 ℃ for 6 hours to 7 days to obtain a fermentation material; Extracting the obtained fermentation material in an extractor to obtain a fermentation extract; And adding a nanoceramic catalyst material and an additive to the fermentation broth to mix and form a fermentation broth composition, wherein the weight ratio of fermentation broth: nanoceramic catalyst material: additive is 100: 1 to 20: 0.1 to 2 in the amount of fermentation broth. It relates to a method for producing a fermentation broth composition for agriculture, including the step of preparing a fermentation broth composition by adding and mixing the nanoceramic catalyst material and additives.

Preferably, the spawn used in the method of the present invention is selected from at least one fermentation fungi consisting of lactic acid bacteria, lactic acid bacteria, yeasts, antibacterial bacteria, nitrogen-fixed bacteria, photosynthetic microorganisms.

The amount of spawn to be inoculated greatly affects fermentation and shortening of fermentation time. If the amount of spawned seed is too small, it will not be effective because the fermentation time will be long. If the amount of spawned seed is too large, the amount of sugar used will increase, resulting in insufficient sugar and fermentation. Consideration should be given to the use of spawn.

Also, preferably, the sugar used in the method of the present invention is selected from the group consisting of oligosaccharides, monosaccharides, disaccharides and polysaccharides.

Oligosaccharides are glycosidic linkages of monosaccharides, which refers to sugars ranging from disaccharides consisting of two monosaccharides to ten sugars consisting of ten monosaccharides, and glycoproteins and glycolipids are mostly oligosaccharides. Sucrose and maltose (maltose) , Lactose (lactose) is selected from the group consisting of one or more.

Monosaccharide refers to a saccharide that is a structural unit formed when hydrolyzing polysaccharides (starch cellulose, etc.) with an acid or an enzyme, and is used by selecting one or more from the group consisting of glucose (glucose), fructose, mannose, galactose, and ribose.

Disaccharide refers to a saccharide that makes two molecules of monosaccharides from one molecule by hydrolysis. One or more disaccharides are selected from the group consisting of sucrose (sugar), maltose (maltose) and lactose (lactose).

Polysaccharide refers to a saccharide, in which two or more monosaccharides are glycosidated to form large molecules, and are cellulose, starch, glycogen, chitin, pectin, amino sugar, glycoprotein, glycolipid, galactan, and alginic acid. Use the above selection.

As the content of sugar is closely related to the growth of microorganisms, the difference between fermentation and fermentation period is similar to the use of spawn. In other words, the more sugars to decompose, the faster the number and proliferation of microorganisms.

However, the organic matter contains saccharides that are easy to decompose. When fermentation is carried out, when excess sugar is added to the microorganisms, it is decomposed to the microorganisms. Falls in In this case, if the seeds are sown, it is likely to become poor germination, and when the roots are down, the roots may become weak. The amount of sugar should be added in consideration of the amount of fish leaf, mugwort and green tea leaf extract to be fermented and the amount of spawn.

Preferably, the functional ceramic catalyst material used in the method of the present invention is a functional catalyst material of TiO ₂ , Al ₂ O ₃ , SiO ₂ , ZrO ₂ , SrTiO ₃ , SnO _{2 ,} WO ₃ ; The functional catalyst material is at least one selected from the group consisting of a coupling catalyst material of Ti, Si, Al, Ag, Zr, Ge.

The most commonly used ceramic catalyst materials are ZrO ₂ , TiO ₂ , Al ₂ O ₃ , SiO ₂ , and mixtures thereof.

In addition, during the manufacturing process according to the present invention, by adding a microorganism as necessary, it can be prepared environmentally friendly fermentation broth composition.

In the manufacturing process according to the invention, if necessary, proteins, polyphenols, chitosan, aromatic oils; And nonionic surfactants, gold (Au), silver (Ag), nitrogen (N), phosphorus (P), carry (K), sulfur (S), calcium (Ca), selenium (Se), magnesium (Mg) , Iron (Fe), copper (Cu), zinc (Zn), molybdenum (Mo), manganese (Mn), boron (B) or a water-soluble salt thereof may be added to the process regardless of the procedure.

In addition, the present invention is manufactured by the method of the present invention, eco-friendly including a functional nano-ceramic catalyst that can maximize the light absorption and light utilization and fermentation extract and photosynthetic microorganism and light fermentation and fermentation of wormwood or fish vinegar and green tea leaf extract A fermentation broth composition is provided.

Eco-friendly fermentation broth composition prepared by the method of the present invention can be used as a nutritional agent, microbial agent, root lubricating agent, growth promoting agent of animals, plants by adding additional organic and inorganic additives.

Mugwort, Echo and green tea leaf extract used in the method of the present invention may use an extract obtained by collecting raw vinegar and mixing with a conventional blender, or when the raw vinegar cannot be collected depending on the season, an extract commercially available may be used. Can be.

Mugwort, Echo and green tea leaves are best used immediately after collecting the raw vinegar and extracts (ie, juice) extracted from the mugwort, Echo and green tea leaves using a conventional blender, the amount of the extract used to prepare a fermentation extract It is used to be 20wt% to 70wt% of the total content of the fermentation composition used to. More preferably 30 wt% to 50 wt%.

In the preparation process according to the present invention, the appropriate amount of sugar used is 1wt% to 50wt%, suitably 10wt% to 40wt%, more preferably 30wt% based on the total weight of the fermentation composition used to prepare the fermentation extract. To 40 wt% is preferred.

In the fermentation composition used to make the fermentation extract during the manufacturing process, if the content of mugwort, eoseongcho, green tea leaf extract and sugar is high, and the content of water is insufficient, the solution is too thick to extract the fermentation broth, so it is difficult to extract and the loss occurs. It is also possible to add by extraction.

If the total content of the fish and green tea leaf extract used in the fermentation extract in the present invention is high, the number of microorganisms is high, but if the content is low, the number of microorganisms tends to be low, but no rapid change is observed. The number of microorganisms is most affected by the amount of sugar added. When the total content of Eoseongcho, mugwort and green tea leaf extract exceeds 70wt% or the added water is less than 5wt%, the fermentation broth is thickened, so it is difficult to extract the fermentation extract and the loss is large. Since it is possible to see a sharp decrease in the amount of fish vinegar, mugwort, green tea leaf extract is preferably adjusted from 20wt% to 70wt%. In addition, green tea leaves are harder to obtain than Eoseongcho and Mugwort, and even if you use a commercially available extract, green tea extract is about 2 to 5 times more expensive than Eoseongcho and Mugwort extracts. It is economically desirable.

Regarding the addition of sugar, when no sugar was added, there was a marked difference in viable cell count than when added, and when the content of sugar was more than 50wt%, the liquid became thicker and no significant increase in the number of bacteria was found. In the manufacturing process, 150wt% of the total weight of the fermentation composition used to make the fermentation extract is preferable. And 530wt% is more preferable in order to easily obtain the fermentation broth and reduce the loss.

When the fermentation broth is more than 50wt%, the liquid is too thick to extract and the loss occurs, so it is possible to extract by adding water when extracting the fermentation broth.

During the production process according to the present invention, photosynthetic microorganisms among the spawn used may be selected from the group consisting of red sulfur bacteria, red non-sulfur bacteria and green sulfur bacteria, but more preferably red non-sulfur bacteria.

Red non-sulfur bacteria are bacteria that feed organic matter under the surface of freshwater lakes or ponds and can grow in a range of anaerobic or aerobic conditions.

In agriculture, green sulfur bacteria, red sulfur bacteria and red non-sulfur bacteria are used, and they grow well under aerobic, anaerobic, light and matt conditions using sugars, and use light energy under anaerobic conditions. Although carbon is fixed by hydrogen or an organic substance as a hydrogen donor, organic substances such as lower fatty acids may be used as the carbon source.

The amount of inoculum used is not a big problem. Even if only a small amount of inoculation in about 57 days because the number of microbial growth is enough to ferment. However, depending on the amount of spawn seed to be inoculated, fermentation and shortening of fermentation time are greatly influenced, so the amount of inoculated bacteria should be adjusted and used according to the fermentation period.

At the time of fermentation, the temperature at the time of fermentation is 20 ° C. to 60 ° C., preferably 30 ° C. to 40 ° C., and the fermentation time is appropriately 6 hours and 7 days, preferably 34 days. If the temperature is too high or too low, fermentation will not work properly. There is a risk of corruption when it is over 60 ℃ and management is important not to rise above 60 ℃ if the fermentation is going on for a long time. Even if the temperature is lower than 20 ℃, it is possible to culture, but the growth of bacteria is too late, so the fermentation time is not efficient. However, when the inoculation bacteria are photosynthetic microorganisms, the photosynthetic microorganisms in culture are killed when the temperature exceeds 40 ° C., so it is advisable to maintain 30 ° C. 35 ° C. while being careful not to exceed 40 ° C. during fermentation.

In the extraction step, the fermentation extract may use both a rotary extractor and a pressure extractor, and there are no special conditions to be careful when extracting.

Functional ceramic catalyst used in step 4 is 0.1wt% to 20wt%, suitably 0.5wt% to 10wt%, more preferably 1wt% to 5wt% based on the total weight of the fermentation composition used to obtain the fermentation extract desirable. If less than 0.1wt%, the amount of ceramic catalyst is too small to be applied after the dilution and fertilization, and when it is more than 20wt%, it causes precipitation, causing stability and unstable stability. The effect is reduced.

Another variable is the stirring speed when mixing, but the reaction proceeds well in the mildest possible conditions, so the speed does not have to be too high. In addition, it is highly stable to stir while slowly dropping the functional ceramic catalyst into the liquid phase.

Eco-friendly fermentation broth composition prepared by the above method has the effect of promoting the growth of crops by strengthening light absorption, water absorption, root swelling and skeleton.

Preferably, in the preparation of the fermentation broth composition of the present invention, fermentation may be performed at 30 ° C. for 3 days.

In addition, the present invention is prepared according to the method for producing a fermentation broth composition of the present invention, the fermentation extract of mugwort, Echo and green tea leaf extract and the nanoceramic catalyst material and additives fermentation extract: nanoceramic catalyst material: additive weight ratio It provides an agricultural fermentation broth composition comprising the amount of 100: 1 to 20: 0.1 to 2.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are intended to illustrate the present invention in more detail, and the scope of the present invention is not limited to these examples.

Reference Example  One

In order to know the composition of the mugwort and eoseongcho, green tea leaf extract used in the preparation of the fermentation broth composition of the present invention and the proper amount of sugar, the content is adjusted differently as shown in Table 1 to prepare and culture the fermentation broth as in Example 1. The viable cell count was then measured.

TABLE 1

(Unit: weight%) Eoseongcho, mugwort, green tea leaf Red non-sulfur bacteria Eocho juice Mugwort juice Green Tea Leaf Juice glucose water Culture Viable cell count after incubation Reference Example 1-1 10 24 5 0 60 One 0.81 * 10 ⁶ CFU / g Reference Example 1-2 10 24 5 One 59 One 1.62 * 10 ⁶ CFU / g Reference Example 1-3 10 24 5 5 55 One 1.86 * 10 ⁶ CFU / g Reference Example 1-4 10 24 5 10 50 One 2.26 * 10 ⁶ CFU / g Reference Example 1-5 10 24 5 20 40 One 2.38 * 10 ⁶ CFU / g Reference Example 1-6 10 24 5 30 30 One 2.43 * 10 ⁶ CFU / g Reference Example 1-7 10 24 5 50 10 One 2.47 * 10 ⁶ CFU / g Reference Example 1-8 10 24 5 60 0 One 2.48 * 10 ⁶ CFU / g Reference Example 1-9 20 24 5 30 20 One 2.43 * 10 ⁶ CFU / g Reference Example 1-10 30 24 5 30 10 One 2.44 * 10 ⁶ CFU / g Reference Example 1-11 10 34 5 30 20 One 2.44 * 10 ⁶ CFU / g Reference Example 1-12 10 14 5 30 40 One 2.42 * 10 ⁶ CFU / g Reference Example 1-13 10 24 15 30 20 One 2.43 * 10 ⁶ CFU / g Reference Example 1-14 20 24 20 30 5 One 2.45 * 10 ⁶ CFU / g Reference Example 1-15 20 24 20 10 20 One 2.31 * 10 ⁶ CFU / g Reference Example 1-16 20 24 20 5 25 One 2.11 * 10 ⁶ CFU / g Reference Example 1-17 20 24 20 One 29 One 1.80 * 10 ⁶ CFU / g

When no sugar was added, the number of viable cells was more pronounced than when added. The sugar content was greater than 50wt%, the liquid became thicker, and no significant increase in the number of viable cells was observed. The content is preferably 5 ~ 50wt% in order to obtain a fermentation extract easily, to reduce the loss.

In Table 1, when the total content of Eoseongcho, mugwort and green tea leaves decreases, the number of bacteria is small, and when the total content of Eoseongcho, mugwort and green tea leaf extract is high, the number of bacteria is increased, but there is no big difference. Therefore, the activity or the number of bacteria is more sensitive to the content of sugar used as energy than the contents of Eoseongcho, mugwort and green tea leaves, and the change is severe.

When the content of sugar is more than 50wt%, the liquid is too thick to extract and difficult to extract, it is also possible to extract by adding water when extracting fermentation broth.

Reference Example  2

Inorganic nanoceramic sol contained in the preparation of the agricultural fermentation broth composition was prepared as follows and used in an experiment.

200 g of ethanol was added to 230 g of titanium ethoxide in a 3 L glass reactor. 0.1 ml of 35% nitric acid was diluted in 200 g of distilled water, and the mixture was sufficiently mixed with stirring gradually, and the first reaction was performed at 50 ° C. for 1 hour. After the first reaction, 0.1 g of iron nitrate [Fe (NO 3) 39 H 2 O] was dissolved in distilled water 10, and 61 g of tetraethoxysilane (TEOS) was slowly added with stirring while stirring. The solution was stirred for 6 hours at a temperature of 90 ℃. After the reaction was stabilized for 2 hours while maintaining the temperature 40 ℃, the reaction was terminated, the nanoceramic sol prepared after the reaction was terminated was used in Example 1.

When the content of the inorganic nanocerasol is less than 0.1wt%, when the fermentation broth composition is to be diluted and fertilized later, the amount can be much smaller than 0.1wt%. The more you enter, the better, but if you enter more than 20wt%, it causes sedimentation. Most preferred is 1wt% to 5wt%

Example  One

Experimental conditions : For the experiment, Eoseongcho, mugwort and green tea leaf raw vinegar were put into a green juice extract, and Eoseongcho, mugwort and green tea leaf juice were prepared. In addition, for comparison experiments, a certain amount of red non-Sulfur bacteria bacteria containing 10 ⁶ CFU / g of photosynthetic microorganisms cultured with a microorganism incubator and a certain amount of sugar was used. Distilled water was used as the water, and the culture temperature was 30 days at 30 ° C., which is an appropriate temperature for photosynthetic microorganisms. After incubation, the cells were diluted to different concentrations, and colony counts were calculated and averaged.

Preparation of fermentation extract : in the amount as shown in Table 2, after mixing the vinegar juice, mugwort, green tea leaf juice in a 20L container and evenly sprayed with an aqueous solution of glucose dissolved in water. Then, a non-sulfur bacterium culture medium of 10 ⁵ to 10 ⁶ CFU / g of photosynthetic microorganisms cultured with a microorganism incubator of MS-150 was sprayed. The temperature inside the vessel was maintained for 3 days while maintaining 30 ℃. The fermented product was put into a rotary extractor to extract the fermentation broth.

TABLE 2

(Unit: weight%) Eoseongcho, mugwort, green tea leaf Red non-sulfur bacteria Eocho juice Mugwort Green Tea Leaf Juice glucose water Culture Viable cell count after incubation Example 1-1 5 5 5 30 54 One 2.30 * 10 ⁶ CFU / g Example 1-2 10 10 5 30 44 One 2.39 * 10 ⁶ CFU / g Example 1-3 20 20 5 30 24 One 2.42 * 10 ⁶ CFU / g Example 1-4 20 20 5 40 14 One 2.46 * 10 ⁶ CFU / g Examples 1-5 20 20 5 50 4 One 2.48 * 10 ⁶ CFU / g Example 1-6 15 15 5 60 4 One 2.47 * 10 ⁶ CFU / g Example 1-7 20 20 5 0 54 One 1.11 * 10 ⁶ CFU / g Example 1-8 15 20 10 30 24 One 2.43 * 10 ⁶ CFU / g Example 1-9 15 15 15 30 24 One 2.42 * 10 ⁶ CFU / g Example 1-10 25 15 5 30 24 One 2.43 * 10 ⁶ CFU / g Example 1-11 10 10 25 30 24 One 2.41 * 10 ⁶ CFU / g Example 1-12 20 20 5 5 49 One 1.90 * 10 ⁶ CFU / g Example 1-13 20 20 5 10 54 One 2.27 * 10 ⁶ CFU / g Example 1-14 20 20 5 20 34 One 2.35 * 10 ⁶ CFU / g Comparative Example 1 35 5 5 30 24 One 2.38 * 10 ⁶ CFU / g Comparative Example 2 5 35 5 30 24 One 2.41 * 10 ⁶ CFU / g Comparative Example 3 5 5 35 30 24 One 2.40 * 10 ⁶ CFU / g

Preparation of fermentation liquid preparation: the above prepared Examples 1-1 to 1-14 and Comparative Examples, each fermentation extract the sugar with stirring with a stirrer 100g fermentation extract molybdenum and 0.01g Borax 0.1g, SiO ₂ 1g and 2g of ceramic sol were slowly added dropwise and mixed. In this way, a fermentation broth composition was prepared.

In the present invention, when the amount of Echo-cho and green tea leaf extract used to prepare the fermentation extract decreases, the number of viable cells after fermentation decreases, but the number of viable cells after fermentation tends to increase, but no rapid change is observed. And when the total content of Eoseongcho, mugwort, and green tea leaf extract exceeds 70wt% or the sugar content exceeds 50wt%, the fermentation broth appears to be thickened, so it is difficult to extract the fermentation extract and the loss is large. In addition, since the total content of Eoseongcho, mugwort and green tea leaf extracts is less than 20wt%, the number of viable cells is rapidly decreased, so it is preferable to adjust the total content of Eoseongcho, mugwort and green tea leaf extracts from 20wt% to 70wt%. In addition, green tea leaves are harder to obtain than Eoseongcho and Mugwort, and even if you use a commercially available extract, green tea extract is about 2 to 5 times more expensive than Eoseongcho and Mugwort extracts. It is economically desirable.

Example  2

Lettuce Cultivation Experiment

The fermentation broth composition prepared in Example 1 was sprayed in an amount of (2000 times 3 L per 33 m ² ) immediately after sowing and planting, respectively, to cultivate the leaf lettuce in a plastic house.

Leaf lettuce was grown in the same plastic house without spraying fermentation broth as a control.

Treatment with ripe compost 70㎏ and nitrogen per 33m ² in both the control as fertilizer: phosphoric acid: the weight of potassium in 100: 50: the extent 4.5㎏ mixed in a ratio of 75 were each once fertilization after sowing and full.

Table 3 shows the results of the time taken until the formulation of the lettuce grown using the composition prepared in Example 1 and the first harvesting time.

TABLE 3

Number of days taken
(When 5-6 pieces of main leaves) Days until first harvest
(After formality) Example 1-1 33 24 Example 1-2 31 22 Example 1-3 30 21 Example 1-4 29 21 Examples 1-5 28 20 Example 1-6 30 21 Example 1-7 34 25 Example 1-8 28 20 Example 1-9 30 22 Example 1-10 29 21 Example 1-11 31 23 Example 1-12 32 24 Example 1-13 31 23 Example 1-14 31 22 Comparative Example 1 29 21 Comparative Example 2 29 21 Comparative Example 3 29 20 Control 36 27

(The above values are divided into 5 zones of 10m ² for each control and treatment and averaged after cultivation)

Example 3

As in Example 2, the fermentation broth composition was sprayed in an amount of (1,600 times 3 L per 33 m ² ) immediately after planting and after planting, respectively , to cultivate leaf lettuce in a plastic house. However, unlike Example 2, only the fermentation broth prepared in Example 1 was grown by spraying without manure.

Table 4 shows the results of the time taken for the lettuce to be cultivated and the time taken for the first harvest after the cultivation.

[Table 4]

Days to set meal (when 5-6 pieces of main leaves) Days to First Harvest (After Formulation) Example 1-1 36 25 Example 1-2 34 25 Example 1-3 32 23 Example 1-4 31 23 Examples 1-5 30 23 Example 1-6 32 24 Example 1-7 34 26 Example 1-8 30 22 Example 1-9 31 23 Example 1-10 32 23 Example 1-11 33 22 Example 1-12 32 24 Example 1-13 33 23 Example 1-14 34 24 Comparative Example 1 33 24 Comparative Example 2 33 23 Comparative Example 3 33 23 Control 36 27

(The above values are divided into 10 zones of 17m ² for each control and treatment and averaged after cultivation)

As can be seen in Table 3 it can be seen that the composition of the present invention can be formulated or harvested about 4-7 days faster than the control. In addition, as shown in Table 4, even when only the fermentation broth except the manure given to the control was treated than the control period and harvesting time was faster. As a result, when the composition of the present invention is used, the cultivation period and the harvesting period can be reduced, and it is confirmed that the eco-friendly fermentation liquid of the present invention is effective. When the control group treated with the fermentation broth was harvested and compared on the same day, the width and color of the leaves were large and thick, and the thickness was thick. Therefore, when treating the environment-friendly fermentation broth of the present invention, the varieties and harvesting time should be well selected to prevent the degradation of lettuce according to the size and thickness.

Example  4

As a laying hen, the feed is considered to be the best (carbohydrates, proteins, vitamins, minerals, etc.) and fed to the newborn female chicks.

The control was fed this best feed, the treatment 1 was fed the best feed, and the fermentation broth composition prepared in Example 1-1 was diluted 100 times with water. Treatment 2 was supplied with a 100-fold dilution of the fermentation broth composition prepared in Example 1-1 in water while feeding a general feed. After about six months, female chicks started laying eggs and the results are shown in Tables 5, 6 and 7.

TABLE 5

Feed efficiency Number of months after spawning Control Treatment (No addition) Treatment tool 1 Treatment 2 Average / column (g) 1 month 46.3 ± 2.0 46.4 ± 0.0 46.2 ± 1.6 2 months 52.6 ± 1.3 53.3 ± 0.9 52.0 ± 1.0 3 months 56.0 ± 0.7 57.3 ± 0.6 56.9 ± 0.0 4 months 59.1 ± 1.0 60.4 ± 0.2 60.1 ± 0.0 5 months 60.9 ± 1.5 61.1 ± 2.5 61.5 ± 0.1 6 months 62.4 ± 1.2 64.3 ± 0.6 63.2 ± 0.7 Average 56.8 ± 1.2 57.4 ± 0.1 57.4 ± 0.0 Indices 100 101.1 101.1 Average weight (g) 0 months 1330 ± 25 1337 ± 67 1368 ± 35 1 month 1526 ± 31 1539 ± 57 1568 ± 28 2 months 1621 ± 58 1641 ± 65 1651 ± 25 3 months 1680 ± 86 1686 ± 41 1730 ± 13 4 months 1702 ± 77 1747 ± 51 1780 ± 23 5 months 1761 ± 67 1774 ± 72 1805 ± 18 6 months 1759 ± 88 1778 ± 85 1816 ± 42

TABLE 6

Average spawn rate (%) Months after treatment Control Treatment (No addition) Treatment tool 1 Treatment 2 1 month 27.2 ± 2.8 37.8 ± 8.2 28.8 ± 4.2 2 months 59.0 ± 4.0 65.6 ± 3.6 63.8 ± 7.0 3 months 63.9 ± 7.6 72.6 ± 7.7 74.5 ± 4.4 4 months 64.2 ± 5.5 73.1 ± 5.6 74.7 ± 3.5 5 months 67.8 ± 1.3 67.2 ± 2.9 74.6 ± 0.9 6 months 63.1 ± 7.0 65.5 ± 3.8 69.1 ± 1.2 Average 56.2 ± 3.4 62.5 ± 3.7 62.8 ± 4.3 Indices 100 111.2 111.7

TABLE 7

 Comparison of Egg Quality Months after treatment Control
Treatment (No addition) Treatment tool 1 Treatment 2   Egg yolk 1 month 7.6 ± 0.6 9.6 ± 0.2 10.5 ± 0.4 2 months 8.0 ± 0.3 10.9 ± 1.0 11.1 ± 1.2 3 months 9.4 ± 1.1 11.5 ± 0.4 12.0 ± 0.4 4 months 9.5 ± 1.6 12.7 ± 0.3 12.6 ± 0.2 5 months 10.2 ± 0.5 12.5 ± 0.3 12.9 ± 0.3 6 months 9.5 ± 0.9 12.5 ± 0.2 12.7 ± 0.1

In the experiment of Example 4, it was observed that the treatment started spawning about one week earlier than the control. The egg production rate was increased by several ten percent than the control group (this experiment was repeated three times with 40 animals at each time, 200 animals at the second time and 1,000 animals at the third time, but showed the same trend).

Then, comparing the quality of the eggs, the yellow color of the yolk of the treatment was very good because the carotene content is increased. Interestingly, the yolks of the treatments were generally larger and the shelf life was longer. This is because the egg white surrounding the egg yolk is surrounded freshly. When the preservation period is long, the egg white will be released little by little. When the whole egg white becomes loose, eventually the egg yolk will rupture, and the egg will start to decay. will be. On the other hand, there is a microorganism that likes odorous substances and removes odors by using them. The representative one is photosynthetic microorganisms. The fermentation broth composition of the present invention contains nanoceramic and photosynthetic microorganisms that can promote light absorption and decomposition, which is more effective.

Example  8

In piglet production pigs, a spraying device is installed for controlling the temperature in pigs during the summer season, and the liquid of the fermentation broth composition prepared in Example 1-1 of the present invention is sprayed on the piglet and sprayed 100-fold diluent in the pig house. Sprayed. In order to confirm the odor removal, the managers and ten people who manage pigs and pig farms were allowed to enter before and after spraying to confirm that the smell of pigs was reduced in smell.

Claims (19)

As a method for preparing a fermentation broth composition for agriculture, the mixture of mugwort, Echo and green tea leaf extract and sugar in an aqueous solution dissolved in water, and spawned with a spawn culture solution to the mixture, fermented at 20 to 60 ℃ for 6 hours to 7 days Obtaining a fermentation material, extracting the obtained fermentation material from an extractor to obtain a fermentation extract, and adding and mixing a nanoceramic catalyst material and an additive to the fermentation broth to form a fermentation broth composition, the fermentation extract: nanoceramic catalyst material To prepare a fermentation broth composition for agriculture, comprising the step of adding a fermentation broth to the fermentation broth in an amount of 100: 1 to 20: 0.1 to 2 by weight of the additive and mixing the additive to produce a fermentation broth composition Way. The method of claim 1, wherein the content of the ingredients in the fermentation step is 15 to 35% by weight, 20 to 30% by weight of mugwort, 5 to 15% by weight of green tea, 5 to 30% by weight of glucose, and 20 to 25% by weight of water. Way The method according to claim 1, wherein the spawn is at least one selected from the group consisting of lactic acid bacteria, lactic acid bacteria, and photosynthetic microorganisms. 4. The method according to claim 3, wherein the photosynthetic microorganism is at least one photomicrobial microorganism selected from the group consisting of red non-sulfur bacteria, red sulfur bacteria, and green sulfur bacteria. The method of claim 1 wherein the additive is at least one component selected from the group consisting of iron, manganese, boron, iron, zinc, copper, molybdenum, magnesium, and water soluble salts thereof. The method of claim 1 wherein the ceramic catalyst material is selected from the group consisting of TiO ₂ , Al ₂ O ₃ , SiO ₂ , ZrO ₂ , SrTiO ₃ , SnO _{2 ,} and WO ₃ . The method of claim 1 wherein the fermentation is carried out at 35 ° C. for at least two days. The method of claim 1, wherein the aqueous solution of sugar is an aqueous solution of 30 to 40% by weight based on the total weight of the fermentation composition, wherein the sugar is oligosaccharide, or at least one selected from the group consisting of monosaccharides, disaccharides, and polysaccharides. The method of claim 8, wherein the oligosaccharide is sucrose, maltose (maltose), or lactose (lactose). The method of claim 8, wherein the monosaccharide is glucose (glucose), fructose, mannose, galactose, or ribose, and the disaccharide is sucrose (sugar), maltose (maltose), or lactose (lactose). And the polysaccharide is cellulose, starch, glycogen, chitin, pectin, aminosaccharides, glycoproteins, glycolipids, galactan, or alginic acid. The fermentation extract, nanoceramic catalyst material and additives of the mugwort, eosungcho and green tea leaf extract are prepared by the method according to claim 1, and the weight ratio of fermentation extract: nanoceramic catalyst material: additive is 100: 1-20: 0.1-2. Agricultural fermentation broth composition containing the amount. The fermentation broth composition of claim 11, wherein the additive is at least one component selected from the group consisting of iron, manganese, boron, iron, zinc, copper, molybdenum, magnesium, and water soluble salts thereof. The fermentation broth composition according to claim 11, wherein the ceramic catalyst material is selected from the group consisting of TiO ₂ , Al ₂ O ₃ , SiO ₂ , ZrO ₂ , SrTiO ₃ , SnO _{2 ,} and WO ₃ . 12. The composition of claim 11, further comprising at least one component selected from the group consisting of gold, silver, protein, polyphenols, chitosan, and aromatic oils; And at least one dispersant selected from the group consisting of nonionic surfactants, anionic surfactants, and amphoteric surfactants. The method of claim 11, wherein gold (Au), silver (Ag), nitrogen (N), phosphorus (P), carry (K), sulfur (S), calcium (Ca), selenium (Se), magnesium (Mg) Agricultural fermentation broth composition further comprising a component selected from the group consisting of iron (Fe), copper (Cu), zinc (Zn), molybdenum (Mo), manganese (Mn), boron (B) and water-soluble salts thereof . 12. The fermentation broth composition for agriculture according to claim 11, further comprising at least one binding material selected from the group consisting of Ti, Si and Ge. The fermentation extract, nanoceramic catalyst material and additives of the mugwort, eosungcho and green tea leaf extract are prepared by the method according to claim 1, and the weight ratio of fermentation extract: nanoceramic catalyst material: additive is 100: 1-20: 0.1-2. Plant root lubricant containing in quantity. The fermentation extract of the mugwort, eoseongcho and green tea leaf extract and the nanoceramic catalyst material and additives are prepared by the method according to claim 1, and the weight ratio of fermentation extract: nanoceramic catalyst material: additive is 100: 1 to 20: 0.1 to 2. Deodorant included in amounts. The fermentation extract, nanoceramic catalyst material and additives of the mugwort, eoseongcho and green tea leaf extract are prepared by the method according to claim 1, and the weight ratio of fermentation extract: nanoceramic catalyst material: additive is 100: 1-20: 0.1-2. Animal and plant nutritional supplements.