KR102039500B1 - Preparation method for ion-organic mineral - Google Patents

Abstract

The present invention relates to a method for producing an ion-organized mineral that incubates the rumen in a medium containing mineral powder, and chelates the mineral powder. (Selenomonas ruminantium), Megasphaera elsdenii, Wallinella succinogenes, Veillonella alcalescens, Vibrio gazogenes, Propionibactium Serpibizer (Propionibacterium cerevisiae), Saccharomyces cerevisiae (Saccharomyces cerevisiae) is utilized.

Description

Preparation method for ion-organic minerals

The present invention relates to a method for producing an ionic organo-passivated mineral that organicizes the mineral obtained in nature so that the absorption rate of the mineral when the animal is ingested.

Minerals are essential nutrients for animal life support. Minerals make up only about 4% of the animal's total composition, but they are essential for the animal's life support and normal physical activity. For example, minerals have been reported to affect the skeletal composition of animals, osmotic pressure regulation, body fluid acid-base equilibrium, enzyme activity, and the like. Therefore, it is widely recognized that minerals may cause problems such as abnormal physiological function and decreased immune function.

However, minerals are not produced naturally in the animal body, so the animal must be ingested through an external plant or food. The natural form of natural minerals obtained in nature is hardly absorbed by the animal's body. In order to absorb minerals in the animal body, there is a method of chelation of minerals with organic molecules, and the applicant has disclosed this method in Korean Patent Publication No. 10-1738475.

Chelating minerals facilitates the absorption in the small intestine of animals. Chelation is a form of coordination of covalent bonds between minerals in the form of metals and organic molecules. Numerous studies have reported that mineral feeds that chelate natural minerals have improved absorption in animals (Baek, 2000, Symposium for the Development of Supplementary Feed Industry: 37-60).

As described above, the chelation of minerals by the chemical method is suitable for industrial mass production, but the organically-purified minerals produced by this method have very high bioavailability because the absorption rate of the animal is about 40%. low. Republic of Korea Patent Publication No. 10-1738475 mentions that there is a limit to the absorption rate of the organic passivated minerals by this chemical method.

Recently, in order to overcome this problem, a method of chelation of minerals has been developed by utilizing the metabolic process of plants. For example, the Republic of Korea Patent Publication No. 10-0893967 utilizes the metabolism of willow mushrooms A method of chelation of minerals is disclosed.

However, the Republic of Korea Patent Publication No. 10-0893967 can improve the absorption of minerals by chelating the growth metabolism process of the willow mushroom, but the growth period of the mushroom is required for the production of a long time to manufacture Since the mushroom spawn is exposed to excess minerals, it is dead, and thus there is a limit in producing a high concentration of ion-organic minerals with high mineral content.

As described above, in order to solve the problems of chemical chelation and chelation using plants, research on a new concept of chelation is urgently needed.

Republic of Korea Patent Publication No. 10-1738475 (Method for manufacturing liquid fertilizer through organicization of natural minerals) Republic of Korea Patent Publication No. 10-0893967 (Cultivation method of willow mushrooms containing mineral and fertilizer using the by-product)

The present inventors have conducted various studies to solve the above problems, and found that ionic organic passivated minerals can be prepared by culturing microorganisms inhabiting ruminants in a medium containing mineral powder.

In order to achieve the above object, the method for producing an ionic organic passivated mineral according to an embodiment of the present invention by culturing the microorganisms inhabit the ruminant (Ruminant stomach) in a medium containing mineral powder to produce an ionic organic passivated mineral do.

The microbes in the rumen include: Selenomonas ruminantium, Megasphaera elsdenii, Wolinella succinogenes, Veillonella alcalescens, Vibrio gazogenes, Propionibacterium cerevisiae, Saccharomyces cerevisiae and at least two or more thereof.

In order to incubate the microorganisms in the rumen with the mineral powder, the medium is composed of 3-7% by weight of rice bran, 3-7% by weight of glucose, 10-40% by weight of mineral powder, 5-15% by weight of organic acid and the remaining amount of water. It can be prepared by sterilizing the mixture.

Method for producing an ionic organic passivated mineral according to an embodiment of the present invention comprises the steps of (A) inoculating and culturing the microorganisms inhabiting the rumen, (B) by obtaining the supernatant located in the upper layer of the culture medium ionic organic Obtaining the passivated mineral, (C) may include the step of sterilizing the ion-organic passivated mineral obtained in the step (B) for 5-7 hours at 50 ~ 80 ℃.

According to the present invention, a method for preparing an ionic organic passivated mineral may be performed by utilizing a microorganism inhabiting the rumen. In addition, the present invention can be used to prepare foods, feeds, and the like containing a higher concentration of ionic organic passivated minerals than chelating methods utilizing the mycelium of plants.

1 is a flow chart for performing a method for producing an ionic organic passivated mineral according to an embodiment of the present invention.
Figure 2 is an image of the process of transferring the ionic organic passivated mineral to rice using the culture medium according to an embodiment of the present invention.

DETAILED DESCRIPTION OF THE INVENTION The following detailed description of the invention has been described in detail by way of specific embodiments in which the invention may be practiced, and in sufficient detail to enable those skilled in the art to practice the invention. It should be understood that the various embodiments of the present invention are different but need not be mutually exclusive. Accordingly, the following detailed description is not to be taken in a limiting sense, and the scope of the present invention should be taken as encompassing the contents described in the claims and all equivalents thereof. Hereinafter, a method for preparing an ion-organized mineral according to the present invention will be described in detail with reference to the accompanying drawings.

1 is a flow chart for performing a method for producing an ionic organic passivated mineral according to an embodiment of the present invention.

Referring to Figure 1, the method for producing an ionic organic passivated mineral according to an embodiment of the present invention is cultured microorganisms inhabit the rumen in a medium containing mineral powder.

The microbes in the rumen are bacteria, protozoa and fungi that inhabit the stomach of ruminants, such as Selenomonas ruminantium, Megasphaera elsdenii, Wallinella succinoge Ness (Wolinella succinogenes), Velonella alcalescens, Vibrio gazogenes, Propionibacterium cerevisiae, Saccharomyces cerevisiae (Saccharomyces) But may be selected from mixtures of at least two or more thereof. The microbes inhabiting the rumen are collected from ruminant animals to obtain pure bacteria through fungal separation, or some microorganisms are sold as products, which are well-known techniques in this field. do.

Ruminants refer to animals that chew by refluxing the food once swallowed, such as cattle, camels, and giraffes. The stomach of ruminants is composed of Rumen, Reticulum, Omasum, and Abomasum. Particularly, the rumen inhabits many microorganisms that degrade cellulose, starch and pectin.

Microorganisms inhabiting the rumen can be prepared by chelating the mineral powder obtained in nature to produce ionic organic passivation minerals, in order to chelate in consideration of the rumen habitat environment of these microorganisms can be carried out by the following manufacturing method.

Method for producing an ionic organic passivated mineral according to an embodiment of the present invention (A) inoculating and culturing the microorganisms inhabiting the rumen in the medium, (B) by obtaining the supernatant located in the upper layer of the culture medium ionic organic Obtaining a passivated mineral, and (C) sterilizing the obtained organic passivated mineral.

In detail by dividing each step, the medium of step (A) can be used as a liquid medium, a medium having a condition that can be cultured rumen microorganisms are required. For example, the production method of the medium is prepared after the mixture consisting of 3-7% by weight of rice bran, 3-7% by weight of glucose, 10-40% by weight of mineral powder, 5-15% by weight of organic acid and the remaining amount of water. The mixture is sterilized by heating to high temperature and high pressure. Prior to sterilizing the mixture, it is preferable to adjust the pH of the mixture to 3 to 6, and if the pH of the mixture is less than 3 or more than 6, the culture rate is drastically decreased or cultured due to inactivation of the microorganisms living in the rumen. May fail.

Therefore, after mixing rice bran, glucose, minerals and water, it is possible to adjust the pH while administering this organic acid. In order to adjust the pH of the mixture to 3 to 6, 5 to 15% by weight of the organic acid may be included relative to the total weight of the mixture. The organic acid is not particularly limited as long as it can adjust the pH of the mixture. For example, the organic acid may be selected from citric acid, malic acid, tartaric acid, succinic acid, and mixtures of two or more thereof.

The mineral is a mineral necessary for the human body, for example, vanadium (V), selenium (Se), germanium (Ge), chromium (C), phosphorus (P), calcium (Ca), sulfur (S), iron (Fe) ), Zinc (Zn), potassium (K), barium (Ba) and mixtures of at least two or more thereof, but are not limited thereto. The mineral may be contained in an amount of 10 to 40% by weight, based on the total weight of the mixture. Chelating of minerals using metabolic processes of plants such as mushrooms usually adjusts the mineral content to 10 to 20% by weight based on the total weight of the medium, because the plant-derived seedlings die when they are overexposed to the minerals. The plant itself is the limit of the absorption of minerals.

However, the method for producing an ionic organic passivated mineral according to the present invention can use the microorganisms inhabiting the rumen can significantly increase the content of minerals in the medium. Therefore, when the content of minerals in the mixture is less than 10% by weight, the mineral content is low, which is not suitable for producing high concentration of ionic organic passivated minerals, and when the content of minerals exceeds 40% by weight, microorganisms inhabiting the rumen to be cultured 'S activity drops significantly.

Sterilization of the mixture may, for example, sterilize the mixture at a temperature of 110-130 for 50-100 minutes. The culture medium described above can be used to culture microorganisms that inhabit the rumen.

Inoculate the microbes in the rumen on the medium. At this time, the content of the inoculated microorganism may be administered 1 to 10% by weight based on the total weight of the medium, if the content of the microorganism is less than 1% by weight it takes a long time to chelate the mineral, the content of the microorganism is 10 If the weight percentage is exceeded, microorganisms may fail due to relatively insufficient nutrients in the culture medium.

In order to make the culture environment into the rumen environment, the medium inoculated with microorganisms can be cultured in an incubator rotating from 30 to 40 to 500 to 1500 rpm, and the incubation temperature is effective for culturing the gastrointestinal environment of the ruminant. On the other hand, when the number of revolutions of the incubator exceeds 1500rpm can be killed microorganisms, if less than 500rpm growth of microorganisms does not occur effectively. The culture solution may be obtained through the inoculation and culturing process described above.

Thereafter, (B) the supernatant of the culture solution can be obtained to obtain the ion-organic passivated mineral. Specifically, in the step (B), the supernatant of the immersion bath is obtained by immersing the culture solution in the immersion tank (B-1). It may include the step (B-2).

Step (B-1) is a process of removing the residue precipitated in the culture. By leaving the culture solution in the immersion tank, and taking only the clear liquid of the upper layer can be obtained chelated ionic organic passivated minerals.

When the ion-organic passivated mineral obtained in step (B) is stored at room temperature, deterioration or decay may occur. Therefore, the method for producing the ion-organic passivated mineral of the present invention may further comprise a sterilization step (C). The sterilization step may sterilize the ionic organic passivated mineral obtained in step (B) at a temperature of 50 to 60 or more for 5 to 7 hours.

The method for preparing the ion-organic mineralized mineral of the present invention will be described by way of example, but not intended to limit the method of the present invention.

In step (A), the threshed rice is administered to the medium containing the mineral powder, and the microorganisms inhabiting the rumen are inoculated into the medium and cultured. In this case, microorganisms can be transferred to grains, vegetables, foods, etc., which can be consumed by animals as well as threshed rice, so that they can contain high concentrations of ionic organic-purified minerals.

-The supernatant obtained from the step (B) is inoculated in a medium containing mineral powder, and cultured by administering rice milled to the medium. In this case, mass production is possible.

-The ionic organic passivated mineral obtained from step (C) can be added to foods, feeds, pesticides, etc. and used industrially.

< Example >

Preparation of the medium

10 kg of citric acid was added to a mixture of 48.2 Kg of purified water, 5 kg of rice bran, 5 kg of glucose, and 30 kg of minerals to adjust the pH of the mixture to 3, and sterilized for 60 minutes in a sterilizer of 121, 1.2 kg / cm ² to prepare a liquid medium. . The mineral was used by uniformly mixing boron (B), magnesium (Mg), calcium (Ca), potassium (K).

Inoculation and culture

Saccharomyces cerevisiae, marketed by Societe Enstriel Resaf (France), was used as a ruminant microorganism. 1.8 kg of Saccharomyces cerevisiae was inoculated into the prepared liquid medium, which was then cultured in an incubator rotating at 37 to 100 rpm.

-Dipping and Supernatant Separation

The supernatant was obtained by placing the cultured culture in an immersion tank for 48 hours and removing the precipitated residue.

< Comparative example  1>

Except for administering the mineral content of the medium at 45kg was carried out in the same manner as in Example, but Saccharomyces cerevisiae was not incubated due to exposure to excess minerals.

< Comparative example  2>

Except for using 1.8 kg of willow mushroom mycelium as a microorganism inoculated into the medium, but was carried out in the same manner as in Example, but will not be cultured by death when the willow mushroom mycelium is exposed to the same amount of minerals as the example.

< Comparative example  3>

Except for using the willow mushroom mycelium as a microorganism inoculated into the medium and the mineral content of the medium was adjusted to 15kg was carried out in the same manner as in Example 1.

The difference between the manufacturing method according to the above-described examples and comparative examples is shown in Table 1 below.

division Example Comparative Example 1 Comparative Example 2 Comparative Example 3 Inoculation microorganism Saccharomyces cerevisiae
1.8 wt% Willow Mushroom Mycelium
1.8 wt% Badge Ingredient

(Wt% of gross weight) Rice bran 5 wt% 5 wt% 5 wt% 5 wt% glucose 5 wt% 5 wt% 5 wt% 5 wt% Organic acid 10 wt% 10 wt% 10 wt% 10 wt% mineral 30 wt% 45 wt% 30 wt% 15 wt% water Remaining amount Remaining amount Remaining amount Remaining amount Ion organic passivated minerals acquired acheive Unacquired Unacquired acheive After transfer to rice, mineral concentration measurement result (per mg / kg)
0.52
-
-
0.12

< Experimental Example >

After transferring the culture solution obtained in Example and Comparative Example 3 to rice, it was intended to measure the mineral content of the rice milled through the component analysis.

The culture solution of Example and the culture solution of Comparative Example 3 were each treated in the same manner as follows.

The threshed rice was sterilized for 90 minutes in a 121, 1.2kg / cm ² pressure sterilizer, and transferred to the cooling chamber to cool down slowly to 20. After placing 30g of sterilized / cooled rice on the Petri dish medium, the medium containing mineral powder was inoculated with the culture medium of Example and the culture medium of Comparative Example 3 to transfer microorganisms to rice to infiltrate ionic organic minerals into rice. I could make it.

Figure 2 is an image of the process of transferring the ionic organic passivated mineral to rice using the culture medium according to the embodiment. Referring to Figure 2, the culture medium of the embodiment is cultured by ion metabolizing the mineral contained in the medium through the metabolic process, and the microorganism permeate the surface layer of rice to transfer the ion-organized mineral into the rice. The rices to which the ionic organosaturated minerals were transferred were washed, sterilized and milled. The milled rice was tested for ingredients to determine the mineral content.

Examples of the mineral content were boron (B) 4,372ppm, magnesium (Mg) 4,414ppm, calcium (Ca) 15,790ppm, potassium (K) 40,390ppm was detected.

As for the mineral content of the comparative example 3, 974 ppm of boron (B), 587 ppm of magnesium (Mg), 9,100 ppm of calcium (Ca), and 25,571 ppm of potassium (K) were detected.

According to the present invention, a method for preparing an ion-organized organic mineral may utilize an microorganism inhabiting the rumen to produce an ion-organized organic mineral that can be absorbed in an animal. In addition, the present invention can be used to prepare foods, feeds, and the like containing a higher concentration of ionic organic passivated minerals than chelating methods utilizing the mycelium of plants.

The present invention described above is not limited to the above-described embodiments, and various substitutions, modifications, and changes can be made without departing from the technical spirit of the present invention. It will be obvious to him.

Claims (7)

A method of producing an ionic organic passivated mineral by culturing microorganisms in ruminant stomach in a medium containing mineral powder,
The microorganism inhabiting the rumen is Saccharomyces cerevisiae (Saccharomyces cerevisiae),
The method for producing the ion organic passivated mineral
(A) inoculating the medium by inoculating microorganisms inhabiting the rumen;
(B) acquiring the supernatant located in the upper layer of the culture solution to obtain an ion-organic passivated mineral; And
(C) sterilizing the ionic organic passivated mineral obtained in step (B) at 50 to 80 ° C. for 5 to 7 hours,
The medium is prepared by sterilizing a mixture consisting of 5% by weight of rice bran, 5% by weight of glucose, 30% by weight of mineral powder, 10% by weight of organic acid and the balance of water,
The medium is a final pH of 3 to 6, after mixing the rice bran, glucose, minerals and water to adjust the pH using an organic acid immediately before sterilization, the method for producing an ionic organic passivated minerals. delete delete delete delete delete The method of claim 1,
Wherein said mineral powder is selected from calcium, potassium, boron, magnesium, vanadium, selenium, germanium, chromium and mixtures of at least two or more thereof.

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