CN103642741A - Compound photosynthetic bacteria agent and preparation method thereof - Google Patents
Compound photosynthetic bacteria agent and preparation method thereof Download PDFInfo
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- CN103642741A CN103642741A CN201310734863.7A CN201310734863A CN103642741A CN 103642741 A CN103642741 A CN 103642741A CN 201310734863 A CN201310734863 A CN 201310734863A CN 103642741 A CN103642741 A CN 103642741A
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- rhodopseudomonas
- microbial inoculum
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Abstract
The invention relates to an agricultural bacterial manure, and particularly relates to a compound photosynthetic bacteria agent and a preparation method thereof. The total effective viable count of the compound photosynthetic bacteria agent is not less than 2*10<8>/ml, wherein the compound photosynthetic bacteria agent contains 25-35 percent of spherical rhodopseudomonas spheroids, 55-65 percent of rhodopseudomonas palustris and 5-15 percent of rhodopseudomonas rubrum. The compound photosynthetic bacteria agent has functions of increasing the yield of vegetables and fruits and improving the quality of the fruits and the vegetables, is capable of reducing the content of heavy metals, is safe in use, and has no problem of pesticide residue. The compound photosynthetic bacteria agent provided by the invention can be prepared by adopting a simple method; a culture medium is not required to be sterilized and subjected to propagation for a plurality of times, and thus the production flow is greatly simplified and the production cost is lowered.
Description
Technical field
The present invention relates to a kind of agriculture microbial-bacterial fertilizer, be specifically related to a kind of Composite Photosynthetic microbial inoculum and preparation method thereof.
Background technology
Microbial-bacterial fertilizer is to make farm crop obtain a kind of goods of specific fertilizer effect by microbial life activity, and crop can obtain specific fertilizer effect and reach promotion plant growth, increases output, improves quality etc.In this effect, the living microorganism in goods plays keying action, and that general microbial fertilizer has is nontoxic, harmless, without the effect of any pollution and promotion plant growth.Photosynthetic bacterium be a class cultivate under given conditions take photosynthetic bacterium as main effective microorganism preparation.Photosynthetic bacterium is because containing abundant nutritive substance and various active material, and oneself knows and unknown function and efficacy to have determined to have many people by photosynthetic bacterium.Research shows: photosynthetic bacterium can be applied the aspects such as agricultural planting, cultivation, food, healthcare products, sewage disposal and new forms of energy, and has higher practical value, and potentiality to be exploited is high.
Rhodopseudomonas is a class photosynthetic bacterium, and it has multiple metabolisable form, has under the condition of illumination or the unglazed photograph of aerobic at aerobic, all can utilize organism to carry out photosynthesis, carries out growth and breeding.And in this bacterioid, contain very abundant all kinds of nutritive substances, in application, as purify water, disease preventing and treating, promotion animal and plant growth etc.The composite fungus agent that contains multiple-microorganism as a kind of in patent CN200910219977.1 open class, can improve output, improve quality, but this composite fungus agent need to be used in conjunction with the materials such as farm manure, humic acid, careless carbonic acid, is mixed more complicated.
The preparation of microbial-bacterial fertilizer often needs to expand and the strict operation such as accompany through strain improvement, classification; and need to be to medium sterilization; carry out with operating under gnotobasis of each step of assurance; operating environment and technician are all had to higher requirement, be unfavorable for cost control and the large-scale production of microbiobacterial agent.Therefore, being necessary to develop a kind of can simplification produces and the microbiobacterial agent favourable to crop growth.
Summary of the invention
The object of present method is to provide a kind of production simple, and substratum, without sterilizing, is mixed convenient and can promotes increasing crop yield, increase quality, the residual Composite Photosynthetic microbial inoculum of agriculture and preparation method thereof of degrading.
Technical scheme of the present invention is as follows.
Total living bacteria count of Composite Photosynthetic microbial inoculum of the present invention is not less than 2 * 10
8individual/ml, wherein contains Rhodopseudomonas spheroides 25 ~ 35%, Rhodopseudomonas palustris 55 ~ 65% and Crimson rhodospirillum 5 ~ 15%.
Total living bacteria count of Composite Photosynthetic microbial inoculum of the present invention is not less than 2 * 10
8individual/ml, wherein contains Rhodopseudomonas spheroides 30%, Rhodopseudomonas palustris 60% and Crimson rhodospirillum 10%.
Composite Photosynthetic microbial inoculum of the present invention also contains substratum, and described substratum contains following component Na
2hPO
40.3g/ml, MgCl
20.12g/ml, NaCl 1.2g/ml, yeast extract paste 1.2g/ml, NaHCO
330g/ml, sodium acetate 1.2g/ml, ammonium sulfate 0.6g/ml, the water of surplus, pH 8.5.
The preparation method of Composite Photosynthetic microbial inoculum of the present invention, is characterized in that it comprises the steps: step 1: pure water is adjusted pH to 8.5; Step 2: with step 1 gained pure water configuration liquid nutrient medium, described substratum contains following component Na
2hPO
40.3g/ml, MgCl
20.12g/ml, NaCl 1.2g/ml, yeast extract paste 1.2g/ml, NaHCO
330g/ml, sodium acetate 1.2g/ml, ammonium sulfate 0.6g/ml; Step 3: access described bacterial classification, always connecing bacterium amount is 2 ~ 4%, and wherein Rhodopseudomonas spheroides 0.6 ~ 1.2%, Rhodopseudomonas palustris 1.2 ~ 2.4%, Crimson rhodospirillum 0.2 ~ 0.4%; Step 4: the bacteria suspension of step 3 gained is sub-packed in narrow-mouthed bottle, and reinforced volume accounts for 70 ~ 85% of described narrow-mouthed bottle volume, is cultured to total living bacteria count with the bulb of 50 ~ 200W and is not less than 2 * 10
8individual/ml.
In the step 3 of Composite Photosynthetic bacterial preparation process of the present invention, always connecing bacterium amount is 3%, and wherein Rhodopseudomonas spheroides 0.9%, Rhodopseudomonas palustris 1.8%, Crimson rhodospirillum 0.3%.
Bulb described in the step 4 of Composite Photosynthetic bacterial preparation process of the present invention is incandescent light bulb, cultivates 3 ~ 5 days.
Total effectively viable bacteria of the present invention refers to the sum of Rhodopseudomonas spheroides, Rhodopseudomonas palustris and three kinds of effective viable bacterias of Crimson rhodospirillum in microbial inoculum.
Positively effect of the present invention is as follows:
Composite Photosynthetic microbial inoculum of the present invention can Degradation and Transformation hazardous and noxious substances, reduces pesticide residue, especially the heavy metals such as lead, cadmium are methylated, thereby reduction toxicity makes its agricultural-food innoxious.
Composite Photosynthetic microbial inoculum of the present invention can improve quality of agricultural product and mouthfeel, shows as: pericarp is thin, pulp is thick, crisp fresh and tender, fruit core is little, color and luster good, the property eaten raw is good, sugar degree is high, storage tolerance, fiber are few, commercial grade is high etc.Especially protein can improve 10% left and right, the corresponding raising of VITAMIN, and sugar degree increases more than 10%, and the hazardous and noxious substances that agricultural chemicals etc. is affected to food has extremely strong degraded, conversion capability, makes agricultural-food innoxious.
Composite Photosynthetic microbial inoculum of the present invention can promote development of plants premature ripening, improves output and unit weight, and extends collection period.Food crop volume increase is more than 10%, and protein can improve 10%; Melon-fruit-like vegetable volume increase is more than 30%, and sugar degree improves more than 10%, and single weight improves more than 10%; The volume increase of stem leafy vegetable more than 30%, can be gone on the market about 7 days ahead of time, and single weight improves more than 10%; Root vegetables volume increase is more than 20%, and monomer weight improves more than 20%; Fruit volume increase more than 20%, is used for many years, and fruit tree can stay fruit more, and fruit-setting rate improves more than 20%, and single fruit weight improves more than 10%, and sugar degree improves more than 15%.And, can solve the problem in trees aging and large and small year.Make fruit tree high and stable yield year after year, trees are younger.Reduce production costs simultaneously, reduce agricultural chemicals usage quantity, reduce vegetables and fruits time and recruitment quantity, improve the nutritive ingredient of fruit.
To sum up, the present invention has the effect that can promote vegetables, fruits output and improve fruit-vegetable quality, and can reduce heavy metal content, uses safety, the problem of non agricultural chemical residuum.And Composite Photosynthetic microbial inoculum of the present invention can be prepared by simple method, substratum, without sterilizing, without through repeatedly expanding and accompany, has greatly been simplified Production Flow Chart and has been reduced production cost.
Accompanying drawing explanation
Fig. 1 is the experimental result picture that Chinese cabbage sprays embodiment 1 and blank;
Fig. 2 is the experimental result picture that Chinese cabbage sprays embodiment 1;
Fig. 3 is the experimental result picture that Chinese cabbage sprays blank;
Fig. 4 is that apple sprays the embodiment experimental result picture in 1 best fruiting period;
Fig. 5 is the experimental result picture that apple sprays the resting stage of embodiment 1 or blank;
Fig. 6 is that apple sprays the embodiment experimental result picture of 1 resting stage;
Fig. 7 is that apple sprays the blank experimental result picture of resting stage.
Embodiment
Embodiment 1
The formula of substratum is Na
2hPO
40.3g/ml, MgCl
20.12g/ml, NaCl 1.2g/ml, yeast extract paste 1.2g/ml, NaHCO
330g/ml, sodium acetate 1.2g/ml, ammonium sulfate 0.6g/ml, the water of surplus, pH 8.5.
Production method:
With former water, by water purification machine, prepare pure water, and carry out pH debugging to 8.5.Then press technical recipe configuration liquid nutrient medium, put into bacterial classification, inoculum size is 3%, and wherein Rhodopseudomonas spheroides 0.9%, Rhodopseudomonas palustris 1.8%, Crimson rhodospirillum 0.3%.
The substratum configuring is sub-packed in 10L narrow-mouthed bottle, and reinforced volume accounts for 85%, 10 bottle a group of described narrow-mouthed bottle volume, is positioned on culturing rack, with 100w incandescent light, cultivates 5 days, and the miscellaneous bacteria rate of finished product is lower than 10% after testing, and finished product is difficult for microbiological contamination, can directly use.
In the composite fungus agent obtaining, total living bacteria count is 2 * 10
8individual/ml, wherein contains Rhodopseudomonas spheroides 30%, Rhodopseudomonas palustris 60%, Crimson rhodospirillum 10%.
Embodiment 2
The formula of substratum is Na
2hPO
40.3g/ml, MgCl
20.12g/ml, NaCl 1.2g/ml, yeast extract paste 1.2g/ml, NaHCO
330g/ml, sodium acetate 1.2g/ml, ammonium sulfate 0.6g/ml, the water of surplus, pH 8.5.
Production method:
With former water, by water purification machine, prepare pure water, and carry out pH debugging to 8.5.Then press technical recipe configuration liquid nutrient medium, put into bacterial classification, inoculum size is 2%, and wherein Rhodopseudomonas spheroides 0.6%, Rhodopseudomonas palustris 1.2%, Crimson rhodospirillum 0.2%.
The substratum configuring is sub-packed in 10L narrow-mouthed bottle, and reinforced volume accounts for 70%, 10 bottle a group of described narrow-mouthed bottle volume, is positioned on culturing rack, with 100w incandescent light, cultivates 5 days, and the miscellaneous bacteria rate of finished product is lower than 10% after testing, and finished product is difficult for microbiological contamination, can directly use.
In the composite fungus agent obtaining, total living bacteria count is 2 * 10
8individual/ml, wherein contains Rhodopseudomonas spheroides 25%, Rhodopseudomonas palustris 65%, Crimson rhodospirillum 10%.
Embodiment 3
The formula of substratum is Na
2hPO
40.3g/ml, MgCl
20.12g/ml, NaCl 1.2g/ml, yeast extract paste 1.2g/ml, NaHCO
330g/ml, sodium acetate 1.2g/ml, ammonium sulfate 0.6g/ml, the water of surplus, pH 8.5.
Production method:
With former water, by water purification machine, prepare pure water, and carry out pH debugging to 8.5.Then press technical recipe configuration liquid nutrient medium, put into bacterial classification, inoculum size is 4%, and wherein Rhodopseudomonas spheroides 0.6%, Rhodopseudomonas palustris 1.2%, Crimson rhodospirillum 0.2%.
The substratum configuring is sub-packed in 10L narrow-mouthed bottle, and reinforced volume accounts for 80%, 10 bottle a group of described narrow-mouthed bottle volume, is positioned on culturing rack, with 100w incandescent light, cultivates 5 days, and the miscellaneous bacteria rate of finished product is lower than 10% after testing, and finished product is difficult for microbiological contamination, can directly use.
In the composite fungus agent obtaining, total living bacteria count is 2 * 10
8individual/ml, wherein contains Rhodopseudomonas spheroides 30%, Rhodopseudomonas palustris 55%, Crimson rhodospirillum 15%.
Experimental example 1: act on vegetables effect of increasing production
Yongqing, Hebei Min Shengyuan Chinese cabbage is on October 6th, 2013, October 31, the Composite Photosynthetic bacterium liquid that twice foliage-spray embodiment 1 makes, 50 times of uses of Composite Photosynthetic microbial inoculum dilution, foliage-spray.The pure water of contrast for spraying under similarity condition.As the Chinese cabbage whole structure figure of Fig. 1, top for spraying embodiment 1, below for spraying contrast, as can be seen from Figure, spray the plant height of experimental group of embodiment 1 apparently higher than control group, and leaf look more emerald green, the cabbage leaf of control group obviously turns to be yellow.As shown in accompanying drawing 2 and 3, to compare with control group, the experimental group Chinese cabbage performance leaf color jade green, bag heart consolidation, single the weight that spray Composite Photosynthetic bacterium liquid increase obviously, and ultimate production improves more than 50%.
Experimental example 2: act on fruit and increase production and improve quality effect
Ridge, Zanhuang, Shijiazhuang, Hebei foundation apple tree sprays the Composite Photosynthetic bacterium liquid that embodiment 2 makes for twice September 29, October 11,50 times of uses of Composite Photosynthetic microbial inoculum dilution, foliage-spray.The pure water of contrast for spraying under similarity condition.As shown in accompanying drawing 4 ~ 7, the experimental group apple performance of Composite Photosynthetic bacterium liquid that sprays embodiment 2 in Fig. 4 is large, fruit is even, bright in colour, sweet crisp, the thick flavor really of mouthfeel, blade emerald green complete, without scab, less, the bud satiation of falling leaves.As Fig. 6, to November 20, the apple tree that sprays embodiment 2 was flourishing, and it is more that fruit tree body stores nutrient, increased winter hardiness, made bud be difficult for being subject to freeze injury, was beneficial to and yielded positive results the coming year.Fig. 7 is contrast fruit tree, and completely, Fig. 5 is whole structure contrast to fallen leaves.
Through Hebei province food quality supervision Inspection Research institute assay: example weight is 1.4Kg, and fructose content is 8.2 g/100g.And the fructose content of blank group is 6.2 g/100g.Test basis is GB5009.93-2010, GB/T 5009.7-2008.
Experimental example 3 pesticide residue and heavy metal detect
Dong Yangshi village, Yuhua District, Shijiazhuang, Hebei Demonstration Garden Pterocladia tenuis Okam. sprays cyhalothrin and Fenvalerate for twice February 14 and March 4; dilute 1500 times; February 15, March 4, spray the Composite Photosynthetic microbial inoculum that embodiment 1 makes for twice; 50 times of uses of Composite Photosynthetic microbial inoculum dilution, foliage-spray.The pure water of contrast for spraying under similarity condition.March 8 was plucked trial plot sample sample presentation, and through Hebei Agricultural Product quality examination Spot detection, cyhalothrin and Cypermethrin do not detect, and plumbous detected level is 0.029mg/Kg, and the detected level of cadmium is 0.0051mg/Kg, far below the minimum standard of national regulation.Test basis is NY/T 761-2008, GB5009.12-2010, GB/T 5009.15-2003.
Experimental example 4 pesticide residue and heavy metal detect
Dong Yangshi village, Yuhua District, Shijiazhuang, Hebei Demonstration Garden crowndaisy chrysanthemum sprays cyhalothrin and Fenvalerate for twice February 14 and March 4; dilute 1500 times; February 15, March 4, spray the Composite Photosynthetic microbial inoculum that embodiment 2 makes for twice; 50 times of uses of Composite Photosynthetic microbial inoculum dilution, foliage-spray.The pure water of contrast for spraying under similarity condition.March 8 was plucked trial plot sample sample presentation, and through Hebei Agricultural Product quality examination Spot detection, cyhalothrin and Cypermethrin do not detect, and plumbous detected level is 0.024mg/Kg, and the detected level of cadmium is 0.0020mg/Kg, far below the minimum standard of national regulation.Test basis is NY/T 761-2008, GB5009.12-2010, GB/T 5009.15-2003.
Experimental example 5 pesticide residue and heavy metal detect
Dong Yangshi village, Yuhua District, Shijiazhuang, Hebei Demonstration Garden Plantula Brassicae chinensis is in spray cyhalothrin and Fenvalerate for twice February 14 and March 4; dilute 1500 times; February 15, March 4, spray the Composite Photosynthetic microbial inoculum that embodiment 3 makes for twice; 50 times of uses of Composite Photosynthetic microbial inoculum dilution, foliage-spray.The pure water of contrast for spraying under similarity condition.March 8 was plucked trial plot sample sample presentation, and through Hebei Agricultural Product quality examination Spot detection, cyhalothrin and Cypermethrin do not detect, and plumbous detected level is 0.016mg/Kg, and the detected level of cadmium is 0.0020mg/Kg, far below the minimum standard of national regulation.Test basis is NY/T 761-2008, GB5009.12-2010, GB/T 5009.15-2003.
Claims (6)
1. a Composite Photosynthetic microbial inoculum, is characterized in that its total living bacteria count is not less than 2 * 10
8individual/ml, wherein contains Rhodopseudomonas spheroides 25 ~ 35%, Rhodopseudomonas palustris 55 ~ 65% and Crimson rhodospirillum 5 ~ 15%.
2. Composite Photosynthetic microbial inoculum according to claim 1, is characterized in that its total living bacteria count is not less than 2 * 10
8/ ml, wherein contains Rhodopseudomonas spheroides 30%, Rhodopseudomonas palustris 60% and Crimson rhodospirillum 10%.
3. Composite Photosynthetic microbial inoculum according to claim 1, is characterized in that it also contains substratum, and described substratum contains following component Na
2hPO
40.3g/ml, MgCl
20.12g/ml, NaCl 1.2g/ml, yeast extract paste 1.2g/ml, NaHCO
330g/ml, sodium acetate 1.2g/ml, ammonium sulfate 0.6g/ml, the water of surplus, pH 8.5.
4. according to a preparation method for the Composite Photosynthetic microbial inoculum described in claim 1 ~ 3 any one, it is characterized in that it comprises the steps:
Step 1: pure water is adjusted pH to 8.5;
Step 2: with step 1 gained pure water configuration liquid nutrient medium, described substratum contains following component Na
2hPO
40.3g/ml, MgCl
20.12g/ml, NaCl 1.2g/ml, yeast extract paste 1.2g/ml, NaHCO
330g/ml, sodium acetate 1.2g/ml, ammonium sulfate 0.6g/ml;
Step 3: access described bacterial classification, always connecing bacterium amount is 2 ~ 4%, and wherein Rhodopseudomonas spheroides 0.6 ~ 1.2%, Rhodopseudomonas palustris 1.2 ~ 2.4%, Crimson rhodospirillum 0.2 ~ 0.4%;
Step 4: the bacteria suspension of step 3 gained is sub-packed in narrow-mouthed bottle, and reinforced volume accounts for 70 ~ 85% of described narrow-mouthed bottle volume, is cultured to total count with the bulb of 50 ~ 200W and is not less than 2 * 10
8/ ml.
5. the preparation method of Composite Photosynthetic microbial inoculum according to claim 4, is characterized in that in step 3, always connecing bacterium amount is 3%, and wherein Rhodopseudomonas spheroides 0.9%, Rhodopseudomonas palustris 1.8%, Crimson rhodospirillum 0.3%.
6. the preparation method of Composite Photosynthetic microbial inoculum according to claim 4, is characterized in that bulb described in step 4 is incandescent light bulb, cultivates 3 ~ 5 days.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105754910A (en) * | 2016-05-12 | 2016-07-13 | 江南大学 | Sprouting vegetable cultivating bacteria agent and application thereof |
| CN108486001A (en) * | 2018-03-13 | 2018-09-04 | 常州大学 | A kind of photosynthetic microbial inoculum of new and effective active selenium-rich and its preparation method and application for paddy growth |
| CN109734196A (en) * | 2019-01-23 | 2019-05-10 | 武汉丰甜生物科技有限公司 | It is a kind of for the Composite Photosynthetic Bacteria microbial inoculum of purification of water quality and its application |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101597579A (en) * | 2009-05-14 | 2009-12-09 | 佛山市顺德区宏隆生物科技有限公司 | A kind of production method of agricultural photosynthetic bacteria preparation |
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2013
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101597579A (en) * | 2009-05-14 | 2009-12-09 | 佛山市顺德区宏隆生物科技有限公司 | A kind of production method of agricultural photosynthetic bacteria preparation |
Non-Patent Citations (3)
| Title |
|---|
| 何若天: "光合细菌在种植业上的应用研究进展", 《广西农业生物科学》 * |
| 王慧娟 等: "光合细菌在农业生产上的应用研究进展", 《安徽农学通报》 * |
| 王秋菊: "光合细菌在植物上的研究现状与展望", 《黑龙江八一农垦大学学报》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105754910A (en) * | 2016-05-12 | 2016-07-13 | 江南大学 | Sprouting vegetable cultivating bacteria agent and application thereof |
| CN105754910B (en) * | 2016-05-12 | 2019-05-17 | 江南大学 | A kind of shoot vegetable cultivates microbial inoculum and its application |
| CN108486001A (en) * | 2018-03-13 | 2018-09-04 | 常州大学 | A kind of photosynthetic microbial inoculum of new and effective active selenium-rich and its preparation method and application for paddy growth |
| CN109734196A (en) * | 2019-01-23 | 2019-05-10 | 武汉丰甜生物科技有限公司 | It is a kind of for the Composite Photosynthetic Bacteria microbial inoculum of purification of water quality and its application |
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