CN103688830A - Method for rapidly rooting and sprouting tissue culture seedlings of dendrobium officinale - Google Patents
Method for rapidly rooting and sprouting tissue culture seedlings of dendrobium officinale Download PDFInfo
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- CN103688830A CN103688830A CN201310651446.6A CN201310651446A CN103688830A CN 103688830 A CN103688830 A CN 103688830A CN 201310651446 A CN201310651446 A CN 201310651446A CN 103688830 A CN103688830 A CN 103688830A
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Abstract
The invention provides a method for rapidly rooting and sprouting dendrobium officinale tissue culture seedlings, which improves the traditional matrix formula and improves the matrix treatment, meanwhile, a plurality of innovations are made on the treatment mode of the tissue culture seedlings, so that the survival rate of the seedlings cultivated by the method is greatly improved to more than 95 percent, the rooting and sprouting time is greatly shortened, and the method is simple, feasible, economical and effective.
Description
Technical field
The present invention relates to plant and cultivate field, refer to especially a kind of method that candidum tissue culturing seedling Rapid Rooting is germinateed.
Background technology
Dendrobium candidum claims again ribbed hedyotis herb, iron sheet orchid etc., medicinal herb grower among the people claims again " the celestial grass of help ", " a thousand pieces of gold grass ", " soft gold " etc., at international medicinal plant circle, be called " medicine circle giant panda ", it belongs to aerial orchid section herbaceous plant, it is China's tradition rare Chinese medicine, successive dynasties ancient books and records: < < Dragon Lord book on Chinese herbal medicine is through > >, < < A Supplement to the Compendium of Materia Medica > > etc. are all on the books, with fresh or dry stem, be used as medicine, have nourishing Yin and clearing heat, nourishing the stomach to improve the production of body fluid, the effect of tonifying kidney and benefiting sperm, but it grows on the unlikely overhanging cliff of birds and beasts, and to temperature, humidity, the weathers such as illumination require to be close to harshness, plant Zhi Hui branch once every year, self-reproduction ability is low, therefore expensive, supply falls short of demand, along with scientific and technical development, people have used biotechnology, by cell, divide culturing method to breed seedlings of Dendrobium officinale, it has solved the problem of plantation seedlings of Dendrobium officinale, but adopt this method also to there is its shortcoming, and water temperature, illumination etc. are also controlled by Computerized intelligent, this a series of technology, growth and the extraneous natural environment of dendrobium candidum are separated, therefore, in order to reduce as much as possible dendrobium candidum field growing environment, , make its from laboratory excessively to the greenhouse-environment of artificial setting, can adapt to as early as possible, and it is fast to root, survive, simple and practical, economical and effective.
Summary of the invention
The present invention proposes a kind of method that candidum tissue culturing seedling Rapid Rooting is germinateed, and has survival rate high, simple, cost-effective feature.
Technical scheme of the present invention is achieved in that a kind of method that candidum tissue culturing seedling Rapid Rooting is germinateed, and comprises the following steps:
(1) matrix is prepared and is processed:
1.1, matrix formulations: mainly by pine bark particle, pelelith particle, the volume ratio of pine bark particle and pelelith particle is 1.5-2.5:1.0;
1.2, matrix treatments: potassium permanganate and ivermectin or Avermectin are diluted to 800 times of aqueous solution, stir, mix rear respectively to pine bark ferment, pelelith soaks, and to matrix is carried out to sterilization, the time is more than 15 days;
1.3, seedbed in matrix: matrix is transported on the seedbed in the booth of building up in advance, pelelith below, thickness 3cm, pine bark up, thickness 7cm, both are laid on seedbed in successively layering;
1.4, matrix is rinsed: with clear water, rinse matrix for three days on end, until outflow is clean water.
(2) preparation and the processing before the plantation of group training seedling:
2.1, hardening: from booth is transferred in laboratory, twist-off closure, allows group training seedling contact first 2-3 days with external environment group training seedling;
2.2, wash seedling: group training seedling is taken out from vial, and clean clean nutrient solution, is listed in bamboo basket by the large, medium and small order of seedling, and thickness is neatly laid in bamboo basket below 2cm;
2.3, sterilization: tpn, by after 1000 times of dilutions, is immersed bamboo basket in tpn solution with clean group training seedling together, continue 5min, then pick up bamboo basket and group training seedling, clean once again in clear water;
2.4, airing: remove group training seedling to sunlight and irradiate 4-5 hour every day, stirred group training seedling once up and down every 1 hour;
(3) cultivation: group training seedling is planted in matrix, and matrix buries root, waters normal root water, till irrigating, until matrix flows down water after field planting is good.
Preferably, in step 1.1, in the formula of matrix, also comprise charcoal, in step 1.3, charcoal is sprinkling upon to the top of pine tree cortex.
Preferably, in step 2.4, control temperature below 35 ℃, the time of irradiation gets between 6-9 point in the morning and between 16 o'clock to 18 o'clock afternoon.
Preferably, group training seedling is planted according to the spacing in the rows of 10*10.
Beneficial effect of the present invention is: the method for candidum tissue culturing seedling Rapid Rooting germination that makes of the present invention is by improving traditional matrix formulations, and carry out perfect to the processing of matrix, simultaneously, in the processing mode of group training seedling, many innovations have been done, make the kind shoot survival percent of cultivating by the method obtain raising greatly, reach more than 95%, and the time of rooting significantly shortens, simple, economical and effective.
Embodiment
For understanding better the present invention; below by following examples, the present invention is done to further concrete elaboration; but unintelligible is limitation of the invention; some nonessential improvement and adjustment of for those skilled in the art, according to foregoing invention content, doing, be also considered as dropping in protection scope of the present invention.
According to method disclosed by the invention, inventor proceeds as follows:
(1) matrix is prepared and is processed:
1.1, matrix formulations: pine bark particle, pelelith particle and charcoal, wherein, the volume ratio of pine bark particle and pelelith particle is 1.5-2.5:1.0;
1.2, matrix treatments: potassium permanganate and ivermectin or Avermectin are diluted to 800 times of aqueous solution, stir, mix rear respectively to pine bark ferment, pelelith soaks and matrix is carried out to sterilization, the time is more than 15 days;
1.3, seedbed in matrix: matrix is transported on the seedbed in the booth of building up in advance, pelelith below, thickness 3cm, pine bark up, thickness 7cm, both are laid on seedbed in successively layering, then charcoal are sprinkling upon to the top of pine tree cortex;
1.4, matrix is rinsed: with clear water, rinse matrix for three days on end, until the water flowing out is clean water.
(2) preparation and the processing before the plantation of group training seedling:
2.1, hardening: from booth is transferred in laboratory, twist-off closure, allows group training seedling contact first 2-3 days with external environment group training seedling;
2.2, wash seedling: group training seedling is taken out from vial, and clean clean nutrient solution, is listed in bamboo basket by the large, medium and small order of seedling, and thickness is neatly laid in bamboo basket below 2cm;
2.3, sterilization: tpn, by after 1000 times of dilutions, is immersed bamboo basket in tpn solution with clean group training seedling together, continue 5min, then pick up bamboo basket and group training seedling, clean once again in clear water;
2.4, airing: remove group training seedling to sunlight and irradiate 4-5 hour every day, stirred group training seedling once up and down every 1 hour, control temperature below 35 ℃, and the time of irradiation gets between 6-9 point in the morning and between 16 o'clock to 18 o'clock afternoon;
(3) cultivation: group training seedling is planted in matrix according to the spacing in the rows of 10*10, and matrix buries root, waters normal root water, until matrix flows down water after field planting is good.
Wherein, in the process of airing, there is following variation in the blade of group training seedling and root:
Night, while there is no solar radiation, blade slowly launched, and when daytime is during solar radiation, blade is rolled again (temperature is not higher than 35 ℃) slowly, after airing is complete, is placed in the ventilation of sheltering from heat or light;
Root, after solar radiation, bleaches gradually within the time of 1-2 days, after 3 days above solar radiation, slowly turns green chap.
More than group training seedling adopts the survival rate of the method to reach more than 95%.
For method of the present invention is done to more detailed contrast, outstanding essentiality content of the present invention, therefore inventor content of the present invention is contrasted with traditional invention, as follows:
In method of the present invention, novelty and bold processing mode, especially first group training seedling is directly placed in and under sunlight, carries out airing, than traditional ventilation of sheltering from heat or light, there is beyond thought outstanding effect, the growth of group training seedling is brought to very desirable influence, be in particular in the growth of root, growing of bud, and in survival rate, all obtained raising, the matrix formulations in the present invention, also compare traditional, component is more, has especially added charcoal, plays distinctive absorption, sterilization, the effect of maintenance microenvironment.
The foregoing is only preferred embodiment of the present invention, in order to limit the present invention, within the spirit and principles in the present invention not all, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (4)
1. a method that makes candidum tissue culturing seedling Rapid Rooting germinate, is characterized in that, comprises the following steps:
(1) matrix is prepared and is processed:
1.1, matrix formulations: be mainly comprised of pine bark particle, pelelith particle, the volume ratio of pine bark particle and pelelith particle is 1.5-2.5:1.0;
1.2, matrix treatments: potassium permanganate and ivermectin or Avermectin are diluted to 800 times of aqueous solution, stir, mix rear respectively to pine bark ferment, pelelith soaks, and matrix is carried out to sterilization, the time is more than 15 days;
1.3, seedbed in matrix: matrix is transported on the seedbed in the booth of building up in advance, pelelith below, thickness 3cm, pine bark up, thickness 7cm, both are laid on seedbed in successively layering;
1.4, matrix is rinsed: with clear water, rinse matrix for three days on end, until the water flowing out is clean water.
(2) preparation and the processing before the plantation of group training seedling:
2.1, hardening: from booth is transferred in laboratory, twist-off closure, allows group training seedling contact first 2-3 days with external environment group training seedling;
2.2, wash seedling: group training seedling is taken out from vial, and clean clean nutrient solution, is listed in bamboo basket by the large, medium and small order of seedling, and thickness is neatly laid in bamboo basket below 2cm;
2.3, sterilization: tpn, by after 1000 times of dilutions, is immersed bamboo basket in tpn solution with clean group training seedling together, continue 5min, then pick up bamboo basket and group training seedling, clean once again in clear water;
2.4, airing: remove group training seedling to sunlight and irradiate 4-5 hour every day, stirred group training seedling once up and down every 1 hour;
(3) cultivation: group training seedling is planted in matrix, and matrix buries root, waters normal root water after field planting is good, irrigates until matrix flows down water.
2. the method that makes candidum tissue culturing seedling Rapid Rooting germinate described in claim 1, is characterized in that: in step 1.1, also comprise charcoal in the formula of matrix, in step 1.3, charcoal is sprinkling upon to the top of pine tree cortex.
3. the method that makes candidum tissue culturing seedling Rapid Rooting germinate described in claim 1, is characterized in that: in step 2.4, control temperature below 35 ℃, the time of irradiation gets between 6-9 point in the morning and between 16 o'clock to 18 o'clock afternoon.
4. the method that makes candidum tissue culturing seedling Rapid Rooting germinate described in claim 1, is characterized in that: in step (3), group training seedling is planted according to the spacing in the rows of 10*10.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104106458A (en) * | 2014-06-28 | 2014-10-22 | 赵木华 | Formula of materials for planting dendrobium candidum |
| CN104303972A (en) * | 2014-10-15 | 2015-01-28 | 霍山县祥英中药材有限公司 | Preparation method of culture medium for dendrobe planting |
| CN104604660A (en) * | 2015-02-11 | 2015-05-13 | 贵州绿春缘有机农业产业管理有限公司 | Imitating wild condition planting method of dendrobium officinale |
| CN104686308A (en) * | 2015-02-11 | 2015-06-10 | 贵州绿春缘有机农业产业管理有限公司 | Cultivation method of Dendrobium officinale Kimura et Migo |
| CN104686207A (en) * | 2015-03-25 | 2015-06-10 | 浙江铁枫堂科技股份有限公司 | Method for cultivating dendrobium officinale |
| CN108651218A (en) * | 2018-05-17 | 2018-10-16 | 中国林业科学研究院林业研究所 | A kind of appropriate substrate of north orchid cultivation |
Citations (3)
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| CN102282998A (en) * | 2011-06-28 | 2011-12-21 | 云南红土生源药用生物科技开发有限公司 | Dendrobium offcinale tissue cultured seedling training and domesticating method |
| CN103053319A (en) * | 2013-01-24 | 2013-04-24 | 云南恒舜生物科技发展有限公司 | Sunshine strong seedling method for dendrobium candidum |
| CN103210846A (en) * | 2013-04-27 | 2013-07-24 | 凌云县长生仙草生物科技开发有限公司 | Method for cultivating dendrobium candidum tissue-culture infected seedlings |
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2013
- 2013-12-06 CN CN201310651446.6A patent/CN103688830B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102282998A (en) * | 2011-06-28 | 2011-12-21 | 云南红土生源药用生物科技开发有限公司 | Dendrobium offcinale tissue cultured seedling training and domesticating method |
| CN103053319A (en) * | 2013-01-24 | 2013-04-24 | 云南恒舜生物科技发展有限公司 | Sunshine strong seedling method for dendrobium candidum |
| CN103210846A (en) * | 2013-04-27 | 2013-07-24 | 凌云县长生仙草生物科技开发有限公司 | Method for cultivating dendrobium candidum tissue-culture infected seedlings |
Non-Patent Citations (1)
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| 韩晓红等: "铁皮石斛生根壮苗培养基优化及移栽基质选择", 《广东农业科学》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104106458A (en) * | 2014-06-28 | 2014-10-22 | 赵木华 | Formula of materials for planting dendrobium candidum |
| CN104303972A (en) * | 2014-10-15 | 2015-01-28 | 霍山县祥英中药材有限公司 | Preparation method of culture medium for dendrobe planting |
| CN104604660A (en) * | 2015-02-11 | 2015-05-13 | 贵州绿春缘有机农业产业管理有限公司 | Imitating wild condition planting method of dendrobium officinale |
| CN104686308A (en) * | 2015-02-11 | 2015-06-10 | 贵州绿春缘有机农业产业管理有限公司 | Cultivation method of Dendrobium officinale Kimura et Migo |
| CN104686207A (en) * | 2015-03-25 | 2015-06-10 | 浙江铁枫堂科技股份有限公司 | Method for cultivating dendrobium officinale |
| CN108651218A (en) * | 2018-05-17 | 2018-10-16 | 中国林业科学研究院林业研究所 | A kind of appropriate substrate of north orchid cultivation |
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| CN103688830B (en) | 2015-11-25 |
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Effective date of registration: 20220510 Address after: 572200 No. 101, 1f, LiMiao youduncao processing plant, kaishi'ercun agricultural products trading center, Fanyang Town, Wuzhishan City, Hainan Province Patentee after: HAINAN LUQING BIOLOGY TECHNOLOGY CO.,LTD. Address before: 572219 Nansheng village, Nansheng Town, Wuzhishan City, Hainan Province Patentee before: HAINAN SONGQING BIOLOGICAL SCIENCE & TECHNOLOGY Co.,Ltd. |