CN103053319A - Sunshine strong seedling method for dendrobium candidum - Google Patents
Sunshine strong seedling method for dendrobium candidum Download PDFInfo
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- CN103053319A CN103053319A CN2013100271367A CN201310027136A CN103053319A CN 103053319 A CN103053319 A CN 103053319A CN 2013100271367 A CN2013100271367 A CN 2013100271367A CN 201310027136 A CN201310027136 A CN 201310027136A CN 103053319 A CN103053319 A CN 103053319A
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Abstract
The invention relates to a sunshine strong seedling method for dendrobium candidum and belongs to the technical field of the dendrobium cultivation. The method includes the steps of rooting culture, week light culture and strong light culture. The sunshine strong seedling method for dendrobium candidum has the advantages that the rooting duration of a test-tube plantlet of the dendrobium candidum is shortened by adjustment of natural light; the quality of the seedlings is greatly improved, the seedlings are strong and trim with strong roots; and after direction plant, the planting survival rate of the seedling is high and reaches over 95%, and the seedlings sprout early.
Description
Technical field
The invention belongs to stem of noble dendrobium artificial cultivation technique field, be specifically related to a kind of sunlight method in strong sprout of dendrobium candidum.
Background technology
The quality of dendrobium officinale test-tube plantlet has a significant impact the growth in its transplanting survival rate and later stage.Therefore, test-tube plantlet is most important when taming the strong sprout in later stage.And in the prior art, normal operation light is cultivated, the seedling of taking root in culturing room, cultivate 3.5 took root to 4 months after, bottle outlet is transplanted to the domestication booth, through the shading hardening about 2 months, could be at field planting, its survival rate is not high, be approximately 60%, and growth cycle is long, production cost is high.Therefore, how to improve the survival rate of seedling quality and plantation seedling, shortening vegetative period just becomes the technical problem that expectation solves.
Summary of the invention
Main purpose of the present invention has provided a kind of survival rate that can significantly improve seedling quality and plantation seedling, shortens the sunlight method in strong sprout of the dendrobium candidum of growth cycle.
The technical solution used in the present invention is a kind of sunlight method in strong sprout of dendrobium candidum, and the method may further comprise the steps:
A, culture of rootage: will cultivate the Dendrobium officinale shoot that highly reaches more than the 3cm through propagation, be inoculated on the MS root media, cultivate 30 days in culturing room, to planting seedling rooting, bottle seedling of must taking root;
B, the low light level are cultivated: bottle seedling of will taking root is transferred to the sunlight booth from culturing room, 22～28 ℃ of temperature, cultivates 30 days under intensity of illumination 5000～8000Lux condition, and to the growth of shoot root system, the leaf look turns green, 6～8 blades of height of seedling 5cm and tool;
C, high light are cultivated: 22～28 ℃ of temperature, cultivated 20 days under intensity of illumination 8000～12000Lux condition, get final product Planting out of test-tube during to height of seedling 7～8cm and 8～10 blades of tool.
As preferably, the condition of culture of culture of rootage is 6～10 hours/every day of illumination among the described step a, intensity of illumination 1000～3000Lux, 22～28 ℃ of cultivation temperature.
As preferably, the condition of culture of culture of rootage is 8 hours/every day of illumination among the described step a, intensity of illumination 2000Lux, 25 ℃ of cultivation temperature.
As preferably, the temperature that the low light level is cultivated among the described step b is 25 ℃.
As preferably, the intensity of illumination that the low light level is cultivated among the described step b is 8000Lux.
As preferably, the temperature that high light is cultivated among the described step c is 28 ℃.
As preferably, the intensity of illumination that high light is cultivated among the described step c is 10000Lux.
Beneficial effect of the present invention is: use by the adjusting of natural lighting (1), can shorten the time that dendrobium officinale test-tube plantlet is taken root; (2) can greatly improve seedling quality, its seedling is sturdy, neat, well developed root system; (3) after seedling was directly planted, its plantation survival rate was high, germinates early, and survival rate reaches more than 95%.
Embodiment
For making those skilled in the art understand in detail production technology of the present invention and technique effect, the below further introduces application of the present invention and technique effect with concrete production instance.
Embodiment one:
To cultivate the Dendrobium officinale shoot that highly reaches more than the 3cm through propagation, be inoculated on the MS root media, cultivate 30 days in culturing room, to planting seedling rooting, bottle seedling of must taking root; Its condition of cultivating in culturing room is 8 hours/every day of illumination, intensity of illumination 2000Lux, 25 ℃ of cultivation temperature.Then carry out the low light level and cultivate, bottle seedling of will taking root is transferred to the sunlight booth from culturing room, 25 ℃ of temperature, cultivates 30 days under the intensity of illumination 8000Lux condition, and to the growth of shoot root system, the leaf look turns green, 6～8 blades of height of seedling 5cm and tool.Carry out again high light and cultivate, 28 ℃ of temperature, cultivated 20 days under the intensity of illumination 10000Lux condition, get final product Planting out of test-tube during to height of seedling 7～8cm and 8～10 blades of tool.
Embodiment two:
To cultivate the Dendrobium officinale shoot that highly reaches more than the 3cm through propagation, be inoculated on the MS root media, cultivate 30 days in culturing room, to planting seedling rooting, bottle seedling of must taking root; Its condition of cultivating in culturing room is 8 hours/every day of illumination, intensity of illumination 1000Lux, 22 ℃ of cultivation temperature.Then carry out the low light level and cultivate, bottle seedling of will taking root is transferred to the sunlight booth from culturing room, 22 ℃ of temperature, cultivates 30 days under the intensity of illumination 5000Lux condition, and to the growth of shoot root system, the leaf look turns green, 6～8 blades of height of seedling 5cm and tool.Carry out again high light and cultivate, 25 ℃ of temperature, cultivated 20 days under the intensity of illumination 8000Lux condition, get final product Planting out of test-tube during to height of seedling 7～8cm and 8～10 blades of tool.
Embodiment three:
To cultivate the Dendrobium officinale shoot that highly reaches more than the 3cm through propagation, be inoculated on the MS root media, cultivate 30 days in culturing room, to planting seedling rooting, bottle seedling of must taking root; Its condition of cultivating in culturing room is 8 hours/every day of illumination, intensity of illumination 3000Lux, 28 ℃ of cultivation temperature.Then carry out the low light level and cultivate, bottle seedling of will taking root is transferred to the sunlight booth from culturing room, 26 ℃ of temperature, cultivates 30 days under the intensity of illumination 6000Lux condition, and to the growth of shoot root system, the leaf look turns green, 6～8 blades of height of seedling 5cm and tool.Carry out again high light and cultivate, 26 ℃ of temperature, cultivated 20 days under the intensity of illumination 12000Lux condition, get final product Planting out of test-tube during to height of seedling 7～8cm and 8～10 blades of tool.
Claims (7)
1. the sunlight method in strong sprout of a dendrobium candidum, it is characterized in that: the method may further comprise the steps:
A, culture of rootage: will cultivate the Dendrobium officinale shoot that highly reaches more than the 3cm through propagation, be inoculated on the MS root media, cultivate 30 days in culturing room, to planting seedling rooting, bottle seedling of must taking root;
B, the low light level are cultivated: bottle seedling of will taking root is transferred to the sunlight booth from culturing room, 22～28 ℃ of temperature, cultivates 30 days under intensity of illumination 5000～8000Lux condition, and to the growth of shoot root system, the leaf look turns green, 6～8 blades of height of seedling 5cm and tool;
C, high light are cultivated: 22～28 ℃ of temperature, cultivated 20 days under intensity of illumination 8000～12000Lux condition, get final product Planting out of test-tube during to height of seedling 7～8cm and 8～10 blades of tool.
2. the sunlight method in strong sprout of a kind of dendrobium candidum according to claim 1, it is characterized in that: the condition of culture of culture of rootage is 6～10 hours/every day of illumination among the described step a, intensity of illumination 1000～3000Lux, 22～28 ℃ of cultivation temperature.
3. the sunlight method in strong sprout of a kind of dendrobium candidum according to claim 2, it is characterized in that: the condition of culture of culture of rootage is 8 hours/every day of illumination among the described step a, intensity of illumination 2000Lux, 25 ℃ of cultivation temperature.
4. the sunlight method in strong sprout of a kind of dendrobium candidum according to claim 1 is characterized in that: the temperature that the low light level is cultivated among the described step b is 25 ℃.
5. the sunlight method in strong sprout of a kind of dendrobium candidum according to claim 1 is characterized in that: the intensity of illumination that the low light level is cultivated among the described step b is 8000Lux.
6. the sunlight method in strong sprout of a kind of dendrobium candidum according to claim 1 is characterized in that: the temperature that high light is cultivated among the described step c is 28 ℃.
7. the sunlight method in strong sprout of a kind of dendrobium candidum according to claim 1 is characterized in that: the intensity of illumination that high light is cultivated among the described step c is 10000Lux.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103688830A (en) * | 2013-12-06 | 2014-04-02 | 海南松青生物科技有限公司 | Method for rapidly rooting and sprouting tissue culture seedlings of dendrobium officinale |
| CN104904597A (en) * | 2015-05-28 | 2015-09-16 | 云南省农业科学院农业环境资源研究所 | Bletilla striata rapid propagation and sunshine seedling strengthening method |
| CN104982169A (en) * | 2015-06-10 | 2015-10-21 | 安徽森亚生态农林有限公司 | Dendrobium officinale cultivation technology |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101564008A (en) * | 2008-04-24 | 2009-10-28 | 中国医学科学院药用植物研究所 | Hormone-free cultivation and rapid propagation method of dendrobium candidum axenic seedlings |
| CN102119655A (en) * | 2010-07-29 | 2011-07-13 | 云南红土生源药用生物科技开发有限公司 | Natural light rapid breeding method for dendrobium officinale |
-
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101564008A (en) * | 2008-04-24 | 2009-10-28 | 中国医学科学院药用植物研究所 | Hormone-free cultivation and rapid propagation method of dendrobium candidum axenic seedlings |
| CN102119655A (en) * | 2010-07-29 | 2011-07-13 | 云南红土生源药用生物科技开发有限公司 | Natural light rapid breeding method for dendrobium officinale |
Non-Patent Citations (3)
| Title |
|---|
| 李桂锋: "铁皮石斛组培快繁工艺及规范化生产体系研究", 《中国优秀硕士学位论文全文数据库（农业科技辑）》 * |
| 潘仕萍 等: "云南珍稀植物铁皮石斛研究初报", 《林业调查规划》 * |
| 赵兴兵 等: "珍稀濒危药材铁皮石斛组培快繁关键技术研究", 《湖南中医药大学学报》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103688830A (en) * | 2013-12-06 | 2014-04-02 | 海南松青生物科技有限公司 | Method for rapidly rooting and sprouting tissue culture seedlings of dendrobium officinale |
| CN103688830B (en) * | 2013-12-06 | 2015-11-25 | 海南松青生物科技有限公司 | Method for rapidly rooting and sprouting tissue culture seedlings of dendrobium officinale |
| CN104904597A (en) * | 2015-05-28 | 2015-09-16 | 云南省农业科学院农业环境资源研究所 | Bletilla striata rapid propagation and sunshine seedling strengthening method |
| CN104982169A (en) * | 2015-06-10 | 2015-10-21 | 安徽森亚生态农林有限公司 | Dendrobium officinale cultivation technology |
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