KR20040065088A - Tissue culture method of Iris sp. - Google Patents

Tissue culture method of Iris sp. Download PDF

Info

Publication number
KR20040065088A
KR20040065088A KR1020030002650A KR20030002650A KR20040065088A KR 20040065088 A KR20040065088 A KR 20040065088A KR 1020030002650 A KR1020030002650 A KR 1020030002650A KR 20030002650 A KR20030002650 A KR 20030002650A KR 20040065088 A KR20040065088 A KR 20040065088A
Authority
KR
South Korea
Prior art keywords
iris
shoots
medium
tissue culture
callus
Prior art date
Application number
KR1020030002650A
Other languages
Korean (ko)
Other versions
KR100491102B1 (en
Inventor
박은필
황수경
Original Assignee
박은필
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 박은필 filed Critical 박은필
Priority to KR10-2003-0002650A priority Critical patent/KR100491102B1/en
Publication of KR20040065088A publication Critical patent/KR20040065088A/en
Application granted granted Critical
Publication of KR100491102B1 publication Critical patent/KR100491102B1/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/002—Culture media for tissue culture
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0025—Culture media for plant cell or plant tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04—Plant cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/34—Sugars
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones

Abstract

PURPOSE: A tissue culture method of Iris sp. is provided, thereby mass-producing Iris sp. within a short time, so that the method is useful for flowering industry. CONSTITUTION: The tissue culture method of Iris sp. comprises the steps of: placing and culturing tissue of Iris sp. on a culturing medium containing 3.0 mg/l of hyponex(20-20-20), 2.0 mg/l of peptone, 80.0 mg/l of adenyl sulfate, 1.0 mg/l of NAA, 1.0 mg/l of BA, 40,000 mg/l of sucrose and 8,000 mg/l of agar and having pH 5.6 to induce calluses of Iris sp.; and transplanting the calluses of Iris sp. on a growth medium containing the same constituents as the culturing medium and culturing them to regenerate shoots of Iris sp., wherein the regenerated shoots of Iris sp. are subcultured at intervals of 45 days.

Description

아이리스의 조직배양방법{Tissue culture method of Iris sp.}Tissue culture method of Iris sp.

본 발명은 아이리스의 조직배양방법에 관한 것으로, 더욱 상세하게는 소독된 아이리스의 조직을 초대배양배지에 치상하여 캘루스를 유기하고, 유기된 캘루스를 증식한 후 증식배지에 치상하여 신초를 재분화시키는 것 또는 아이리스의 측아를 상기 초대배양배지와 동일한 조성 및 pH를 가지는 배지에 치상하여 신초를 재분화시키는 것을 특징으로 하는 아이리스의 조직배양방법에 관한 것이다.The present invention relates to a tissue culture method of the iris, and more specifically, the tissue of the sterilized iris is inoculated on the first culture medium to induce callus, and the expanded callus is grown on the growth medium to redifferentiate shoots. Or it relates to a tissue culture method of the iris characterized in that the side of the iris is placed in a medium having the same composition and pH as the primary culture medium to re-differentiate shoots.

아이리스는 우리말로는 붓꽃, 계손(溪蓀), 수창포, 창포붓꽃이라고 한다. 외떡잎식물 백합목 붓꽃과의 여러해살이풀로서 한국, 일본, 중국 북동부, 시베리아 동부에 분포하고, 산기슭의 건조한 곳이 자생지이며, 높이는 60cm 내외이다. 뿌리줄기가 옆으로 자라면서 새싹이 나와 뭉쳐나며, 밑부분에 붉은빛을 띤 갈색 섬유가있다. 잎은 나비 5∼10mm이고 도드라진 맥이 없으며 밑부분은 잎집처럼 되고 붉은빛이 도는 것도 있다.Iris is called iris, cinnamon, iris, and iris. It is a perennial plant of lily tree Iridaceae, distributed in Korea, Japan, northeastern China and eastern Siberia. The dry area at the foot of the mountain is native, and its height is about 60cm. The rhizome grows laterally, buds come out and clump together, with reddish brown fibers at the bottom. The leaves are 5-10mm of butterfly, there are no raised veins, and the bottom part is leafy and reddish.

꽃은 5∼6월에 피고 자줏빛이며 지름 8cm 정도로 꽃줄기 끝에 2∼3개씩 달린다. 포는 잎처럼 생기고 녹색이며 작은포가 포보다 긴 것도 있다. 작은꽃자루는 작은포보다 짧고 씨방보다 길다. 외화피는 넓은 달걀을 거꾸로 새운 듯한 모양이며 밑부분에 옆으로 달린 자줏빛 맥이 있고 내화피는 곧게 선다.Flowers bloom in May-June, purple, 8 ~ 3 cm in diameter, hang 2-3 at the end of stalk. A bract is leaf-like, green, and a small bract is longer than a bract. A peduncle is shorter than a sackcloth and longer than an ovary. The outer skin is like an upside-down egg with a broad egg, and there is a purple vein on the bottom, and the inner skin stands upright.

열매는 삭과로서 대가 있고 양 끝이 뾰족한 원기둥 모양이다. 종자는 갈색이고 삭과 끝이 터지면서 나온다. 민간에서 뿌리줄기를 피부병·인후염 등에 쓴다.Fruits are capsules, with a stem and pointed cylindrical ends. Seeds are brown and come out with crusts and ends. Folk roots are used for skin diseases and sore throats.

번식은 9월 하순~10월상순경 새싹을 2-3개 붙여 나누는 포기나누기에 의해 수행되며, 흙위로 뿌리가 1/3정도 드러나게 심는다. 비료는 봄,가을로 퇴비나 부엽토를 주고 토양은 물빠짐이 좋은 사질양토로 약알카리성이 되게 하여 사용하며, 직사광선 또는 밝은 빛이 들게 하여 재배한다.Breeding is carried out by abandoning dividing 2-3 sprouts in late September to early October, planting roots 1/3 over the soil. Fertilizers are used in spring and autumn to give compost or foliar soil. Soil is a sandy loam soil with good drainage. It is used to be weakly alkaline and is grown under direct sunlight or bright light.

그러나 상기와 같은 번식법으로는 대량생산에 한계가 있어, 그 대안으로 조직배양에 의한 대량생산 방법을 고려할 수 있는데 이와 관한 종래의 연구는 미미하다.However, the breeding method as described above has a limitation in mass production, and as an alternative, a mass production method by tissue culture can be considered. However, the conventional research on this is insignificant.

이에 본 발명자들은 아이리스에 대한 대량생산방법의 일환으로 아이리스의 조직배양방법을 개발하고자 예의 노력하였으며, 그 결과, 하이포넥스(20-20-20) 3.0 mg/L, 펩톤 2.0 mg/L, 아데네 설페이트 80.0 mg/L, NAA 1.0 mg/L, BA 1.0mg/L, 수크로오스 40,000 mg/L, 아가 8,000 mg/L로 제조된 배지를 이용하여 아이리스의 조직배양방법을 확립함으로써 본원발명을 완성하였다.Therefore, the present inventors made an effort to develop a tissue culture method of the iris as part of the mass production method for the iris, as a result, Hyphenex (20-20-20) 3.0 mg / L, peptone 2.0 mg / L, adene The present invention was completed by establishing a method for tissue culture of iris using a medium prepared with sulfate 80.0 mg / L, NAA 1.0 mg / L, BA 1.0 mg / L, sucrose 40,000 mg / L, and agar 8,000 mg / L.

따라서, 본 발명의 목적은 아이리스의 대량증식을 위한 조직배양방법을 제공하는 것이다.Accordingly, it is an object of the present invention to provide a tissue culture method for mass proliferation of iris.

도 1은 조직배양에 의한 아이리스의 캘루스 증식 사진도이다.1 is a picture of callus proliferation of iris by tissue culture.

도 2는 조직배양에 의한 아이리스의 식물체 분화 증식 사진도이다.2 is a photograph of plant differentiation and proliferation of iris by tissue culture.

상기 목적을 달성하기 위하여 본 발명은 아이리스의 조직을 하이포넥스(20-20-20) 3.0 mg/L, 펩톤 2.0 mg/L, 아데네 설페이트 80.0 mg/L, NAA 1.0 mg/L, BA 1.0 mg/L, 수크로오스 40,000 mg/L, 아가 8,000 mg/L로 제조된 초대 배양배지에 치상하여 캘루스를 유기하고 초대 배양배지와 같은 조성의 증식배지로 옮겨 치상하여 신초를 재분화하는 것을 특징으로 하는 아이리스의 조직배양방법 및 하이포넥스(20-20-20) 3.0 mg/L, 펩톤 2.0 mg/L, 아데네 설페이트 80.0 mg/L, NAA 1.0 mg/L, BA 1.0 mg/L, 수크로오스 40,000 mg/L, 아가 8,000 mg/L로 제조된 배지에 아이리스의 측아를 치상하여 싹을 틔우고, 같은 배지에 옮겨 치상함으로써 신초를 재분화하는 것을 특징으로 하는 아이리스의 조직배양방법을 제공한다.In order to achieve the above object, the present invention provides a tissue of Iris Hyponex (20-20-20) 3.0 mg / L, peptone 2.0 mg / L, adene sulfate 80.0 mg / L, NAA 1.0 mg / L, BA 1.0 mg / L, sucrose 40,000 mg / L, agar 8,000 mg / L, the iris is characterized in that the callus is organic and then transferred to a growth medium of the same composition as the primary culture medium and healed to re-differentiate shoots Tissue culture method and hypoxene (20-20-20) 3.0 mg / L, peptone 2.0 mg / L, adene sulfate 80.0 mg / L, NAA 1.0 mg / L, BA 1.0 mg / L, sucrose 40,000 mg / L , Agar 8,000 mg / L agar of the side of the iris to the teeth of the iris and shoots, transfer to the same medium to provide a tissue culture method of iris, characterized in that to differentiate the shoots.

이하, 본 발명에 대해 더욱 상세히 설명한다.Hereinafter, the present invention will be described in more detail.

본원발명은 본원발명에서 제조된 초대배양배지에서 캘루스를 유기하고, 유기되어 증식된 캘루스를 증식배지에 치상하여 신초를 재분화시키는 것 또는 본원발명에서 제조된 배지에 아이리스의 측아를 치상하여 신초를 재분화 하는 것에 특징이있는 아이리스의 조직배양방법이다. 따라서, 배지를 제외한 전반적인 조직배양방법은 공지의 방법에 의하여 수행한다.The present invention is to induce the callus in the primary culture medium prepared in the present invention, and to re-differentiate shoots by incubating the callus grown organically grown callus in the growth medium or to shoot the iris in the medium prepared in the present invention It is a tissue culture method of iris which is characterized by re-differentiation. Therefore, the overall tissue culture method except the medium is carried out by a known method.

캘루스를 유기할 때는 아이리스에 있어, 캘루스 유기가 가능한 조직이라면 어느 부위를 사용하도 무방하나, 바람직스럽게 아이리스의 화경을 채취하여 사용하는 것이 좋고, 종래의 방법으로 NaOCl에 Tween20을 첨가하여 소독한 후 사용하는 것이 좋으며, 소독된 화경은 멸균수에 헹구어 사용하는 것이 좋다.When the callus is organic, any part of the tissue may be used as long as it is a tissue capable of callus organicity. However, it is preferable to collect and use a flower garden of the iris, and to disinfect by adding Tween20 to NaOCl by a conventional method. It is recommended to use it afterwards, and sterilized flowers should be rinsed in sterile water.

상기와 같이 멸균수에 헹구어진 조직은 바람직스럽게 pH가 5.6으로 조정된 아이리스 초대 배양배지에 치상하는 것이 좋고, 치상한지 약 6 주 후에 캘루스가 유기된다. 한편, 상기 아이리스 초대배양배지는 하이포넥스(20-20-20) 3.0 mg/L, 펩톤 2.0 mg/L, 아데네 설페이트 80.0 mg/L, NAA 1.0 mg/L, BA 1.0 mg/L, 수크로오스 40,000 mg/L, 아가 8,000 mg/L로 제조된 것을 사용한다.Tissues rinsed in sterile water as described above are preferably placed in an iris primary culture medium whose pH is adjusted to 5.6, and the callus is organic after about 6 weeks. On the other hand, the iris initial culture medium hypoxene (20-20-20) 3.0 mg / L, peptone 2.0 mg / L, adene sulfate 80.0 mg / L, NAA 1.0 mg / L, BA 1.0 mg / L, sucrose 40,000 mg / L, agar prepared at 8,000 mg / L is used.

유기된 캘루스를 충분히 증식한 후, 증식배지 옮겨 치상하면 6~7주 후에 신초가 재분화된다. 증식배지는 초대 배양배지와 같은 것을 사용한다.After enough growth of the released callus, the growth medium is transferred and healed, the shoots are re-differentiated after 6 to 7 weeks. Proliferation medium is the same as the primary culture medium.

또한, 본 발명은 아이리스의 측아를 채취하여 상기의 초대 배양배지와 같은 조성 및 같은 pH의 배지에 치상하여 신초를 재분화시킨다. 상기 측아는 바람직스럽게 관행적인 방법으로 NaOCl에 Tween20을 첨가하여 소독하는 것이 좋다.In addition, the present invention is to re-differentiate shoots by taking the side of the iris and denaturing the same composition and the same pH medium as the primary culture medium. The side buds are preferably sterilized by adding Tween20 to NaOCl in a conventional manner.

치상한지 4주 후에 싹이 트고, 이를 같은 배지에 옮겨 치상하면 6~7주 후에 신초가 재분화된다.After 4 weeks of hemorrhage, shoots are sprouted and transferred to the same medium to heal after 6-7 weeks.

상기 캘루스로부터 재분화된 아이리스 신초 또는 측아로부터 재분화된 아이리스 신초는 45일 간격으로 계대배양을 하여 대량증식을 하는 것이 좋다.The iris shoots re-differentiated from the callus or iris shoots re-divided from the side of the callus may be mass-produced by passage at 45-day intervals.

이하, 본 발명의 구성에 대해 하기 실시예를 들어 더욱 상세히 설명하지만, 본 발명의 권리범위가 하기 실시예에만 한정되는 것은 아니다.Hereinafter, the configuration of the present invention will be described in more detail with reference to the following examples, but the scope of the present invention is not limited only to the following examples.

실시예 1: 아이리스로부터 캘루스의 유기 및 신초의 재분화Example 1 Organic and Shoot Regeneration of Callus from Iris

아이리스의 화경을 채취하여 당업계의 통상적인 방법으로 NaOCl에 Tween20을 첨가하여 소독하였다. 소독된 화경을 멸균수에 헹구어 pH 5.6으로 조정된 아이리스 초대 배양배지에 치상하였다. 아이리스 초대 배양배지는 하이포넥스(20-20-20) 3.0 mg/L, 펩톤 2.0 mg/L, 아데네 설페이트 80.0 mg/L, NAA 1.0 mg/L, BA 1.0 mg/L, 수크로오스 40,000 mg/L, 아가 8,000 mg/L를 혼합한 것으로 1 L를 사용하였다.The flower of Iris was collected and sterilized by adding Tween20 to NaOCl according to a conventional method in the art. The sterilized flowers were rinsed in sterile water and placed in iris primary culture medium adjusted to pH 5.6. Iris primary culture medium is 3.0 mg / L hypoxene (20-20-20), peptone 2.0 mg / L, adene sulfate 80.0 mg / L, NAA 1.0 mg / L, BA 1.0 mg / L, sucrose 40,000 mg / L 1 L was used as a mixture of 8,000 mg / L agar.

6주 후에 캘루스가 유기되었으며, 유기된 캘루스를 충분히 증식한 후, 증식배지에 치상하였다(도 1). 증식배지는 상기 초대 배양배지와 동일한 것이었다. 6주 후에 신초가 재분화되었다(도 2).After 6 weeks, callus was incubated, and after the incubated callus was sufficiently grown, it was placed on a growth medium (FIG. 1). The growth medium was the same as the primary culture medium. After 6 weeks shoots were re-differentiated (FIG. 2).

재분화된 신초를 45일 간격으로 계대배양하여 아이리스를 대량생산하였다.Iris was subcultured at 45 days intervals to mass produce iris.

실시예 2: 아이리스의 측아로부터 신초의 재분화Example 2: Regeneration of Shoot from Iris' Side

실시예 1과 같은 방법으로 소독작업을 거친 아이리스 측아를 실시예 1에서 사용한 초대 배양배지와 같은 pH 및 조성을 가지는 배지에 치상하였다. 치상한지 4주 후 싹이 트였고, 이를 같은 배지에 옮겨 치상하였는데 6주 후에 신초가 재분화 되었다. 재분화된 신초를 45일 간격으로 계대배양하여 아이리스를 대량생산하였다.Iris side buds, which had been sterilized in the same manner as in Example 1, were mounted on a medium having the same pH and composition as the primary culture medium used in Example 1. After 4 weeks of hemorrhage, the shoots sprouted and were transferred to the same medium and healed. After 6 weeks, shoots were re-differentiated. Iris was subcultured at 45 days intervals to mass produce iris.

이상, 상기에서 살펴본 바와 같이 본원발명에서 제조된 초대배양배지에서 캘루스를 유기하고, 유기되어 증식된 캘루스를 증식배지에 치상하여 신초를 재분화시키는 것 또는 본원발명에서 제조된 배지에 아이리스의 측아를 치상하여 신초를 재분화 하는 것을 특징으로 하는 본원발명의 아이리스 조직배양방법은 아이리스을 짧은 시간에 대량으로 생산할 수 있으므로 화훼산업상 매우 유용한 것이다.As described above, the callus is organically grown in the primary culture medium prepared in the present invention, and the organically grown callus is grown on the growth medium to re-differentiate shoots or in the medium prepared according to the present invention. Iris tissue culture method of the present invention characterized by re-differentiating shoots by repairing the iris is very useful in the flower industry because it can produce a large amount of iris in a short time.

Claims (5)

아이리스의 조직을 하이포넥스(20-20-20) 3.0 mg/L, 펩톤 2.0 mg/L, 아데네 설페이트 80.0 mg/L, NAA 1.0 mg/L, BA 1.0 mg/L, 수크로오스 40,000 mg/L, 아가 8,000 mg/L로 제조된 초대 배양배지에 치상하여 캘루스를 유기하고 초대 배양배지와 같은 조성의 증식배지로 옮겨 치상하여 신초를 재분화하는 것을 특징으로 하는 아이리스의 조직배양방법.The tissues of the iris were hypoxene (20-20-20) 3.0 mg / L, peptone 2.0 mg / L, adene sulfate 80.0 mg / L, NAA 1.0 mg / L, BA 1.0 mg / L, sucrose 40,000 mg / L, A method for tissue culture of iris, characterized by regeneration of callus by incubating the primary culture medium prepared with agar 8,000 mg / L and transferring the callus to a growth medium having the same composition as the primary culture medium. 제 1항에 있어서, 상기 아이리스의 조직은 화경으로부터 얻어진 것을 특징으로 하는 아이리스의 조직배양방법.The method of claim 1, wherein the tissue of the iris is obtained from a flower garden. 하이포넥스(20-20-20) 3.0 mg/L, 펩톤 2.0 mg/L, 아데네 설페이트 80.0 mg/L, NAA 1.0 mg/L, BA 1.0 mg/L, 수크로오스 40,000 mg/L, 아가 8,000 mg/L로 제조된 배지에 아이리스의 측아를 치상하여 싹을 틔우고, 같은 배지에 옮겨 치상함으로써 신초를 재분화하는 것을 특징으로 하는 아이리스의 조직배양방법.Hyponex (20-20-20) 3.0 mg / L, peptone 2.0 mg / L, adene sulfate 80.0 mg / L, NAA 1.0 mg / L, BA 1.0 mg / L, sucrose 40,000 mg / L, agar 8,000 mg / A method for tissue culture of iris, characterized by replanting the shoots of the iris with a medium made of L, sprouting the shoots, and transferring the same to the same medium to differentiate the shoots. 제 1항 또는 제 3항에 있어서, 상기 배지는 pH가 5.6임을 특징으로 하는 아이리스의 조직배양방법.4. The method of claim 1 or 3, wherein the medium has a pH of 5.6. 제 1항 또는 제 3항에 있어서, 재분화된 신초는 45일 간격으로 계대배양하는 것을 추가로 포함하는 것을 특징으로 하는 아이리스의 조직배양방법.4. The method of claim 1 or 3, wherein the re-differentiated shoots further comprise subcultures at 45 day intervals.
KR10-2003-0002650A 2003-01-15 2003-01-15 Tissue culture method of Iris sp. KR100491102B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR10-2003-0002650A KR100491102B1 (en) 2003-01-15 2003-01-15 Tissue culture method of Iris sp.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR10-2003-0002650A KR100491102B1 (en) 2003-01-15 2003-01-15 Tissue culture method of Iris sp.

Publications (2)

Publication Number Publication Date
KR20040065088A true KR20040065088A (en) 2004-07-21
KR100491102B1 KR100491102B1 (en) 2005-05-24

Family

ID=37355459

Family Applications (1)

Application Number Title Priority Date Filing Date
KR10-2003-0002650A KR100491102B1 (en) 2003-01-15 2003-01-15 Tissue culture method of Iris sp.

Country Status (1)

Country Link
KR (1) KR100491102B1 (en)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103202230A (en) * 2013-04-22 2013-07-17 江苏省中国科学院植物研究所 Rapid propagation method of red-seed iris
CN104996302A (en) * 2015-08-20 2015-10-28 浙江大学 Method for remarkably improving callus inductivity of iris pseudacorus L.
CN108496800A (en) * 2018-04-10 2018-09-07 黑龙江省科学院自然与生态研究所 Chinese small iris high frequency regenerating system method is established based on callus induction
CN109156358A (en) * 2018-09-26 2019-01-08 江苏省中国科学院植物研究所 A kind of method of Iris germanica rataria induced embryonic callus
CN111567401A (en) * 2020-04-20 2020-08-25 上海植物园 Tissue culture rapid propagation method of butterfly flower' Mengdian
CN111887152A (en) * 2019-11-19 2020-11-06 东北林业大学 Method for establishing plant regeneration system of swallow flower bud growing way
CN116034871A (en) * 2022-11-18 2023-05-02 上海纳米技术及应用国家工程研究中心有限公司 Tissue culture rapid propagation method of glabrous tarragon

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007061146A1 (en) * 2005-11-22 2007-05-31 Chung-Ang University Industry-Academy Cooperation Foundation A method for producing chinese cabbage transformant using tissues of flower stalk and a transformant with promoted soft rot resistance obtained from the method
KR100819878B1 (en) 2007-06-26 2008-04-08 중앙대학교 산학협력단 A method for producing Chinese cabbage transformant using tissues of flower stalk and a transformant with promoted soft rot resistance obtained from the method
CN102893872A (en) * 2012-11-03 2013-01-30 云南省农业科学院花卉研究所 Tissue culture method for domesticated seedlings of iris pallida
EP4342448A1 (en) 2021-08-18 2024-03-27 Exocobio Inc. Novel composition comprising iris-derived exosome as active ingredient

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103202230A (en) * 2013-04-22 2013-07-17 江苏省中国科学院植物研究所 Rapid propagation method of red-seed iris
CN104996302A (en) * 2015-08-20 2015-10-28 浙江大学 Method for remarkably improving callus inductivity of iris pseudacorus L.
CN108496800A (en) * 2018-04-10 2018-09-07 黑龙江省科学院自然与生态研究所 Chinese small iris high frequency regenerating system method is established based on callus induction
CN108496800B (en) * 2018-04-10 2021-07-16 黑龙江省科学院自然与生态研究所 Method for establishing iris high-frequency regeneration system based on callus induction
CN109156358A (en) * 2018-09-26 2019-01-08 江苏省中国科学院植物研究所 A kind of method of Iris germanica rataria induced embryonic callus
CN111887152A (en) * 2019-11-19 2020-11-06 东北林业大学 Method for establishing plant regeneration system of swallow flower bud growing way
CN111887152B (en) * 2019-11-19 2022-06-07 东北林业大学 Method for establishing plant regeneration system of swallow flower bud growing way
CN111567401A (en) * 2020-04-20 2020-08-25 上海植物园 Tissue culture rapid propagation method of butterfly flower' Mengdian
CN116034871A (en) * 2022-11-18 2023-05-02 上海纳米技术及应用国家工程研究中心有限公司 Tissue culture rapid propagation method of glabrous tarragon

Also Published As

Publication number Publication date
KR100491102B1 (en) 2005-05-24

Similar Documents

Publication Publication Date Title
CN102119660B (en) Method for rooting culture,seedling adaptation and transplantation of alpine rose tissue-cultured seedling in greenhouse
CN111213504B (en) Woody stock for efficient grafting of solanaceous vegetables and efficient grafting seedling method thereof
CN107926715A (en) A kind of eggplant or/and the engrafting and cultivating method of capsicum or/and tomato
CN102845313A (en) Method for quickly in-vitro actinidia kolomikta propagating
CN112586346B (en) Tree eggplant, and cultivation method, rapid propagation method and application thereof
KR100491102B1 (en) Tissue culture method of Iris sp.
CN101133704A (en) Sterilized spore germination of platycerium wallichii and test tube seedling cultivating method
CN102986535B (en) Fast propagation method of seedless roxburgh rose seedlings
CN103609444B (en) Tissue culture method for hemerocallis sempervirens araki
CN106718763B (en) Sedum short-shoot cutting cultivation method
CN103283504B (en) Method for grafting pear polyploidy test-tube plantlet outside test tube
CN105660391A (en) Tissue culture breeding method for apple sapling
CN105028193A (en) Breeding method for generating micro adventitious buds through induction of legacy leaves
CN105145363A (en) Method for significantly improving fir tissue culture production emergence rate
CN113197091A (en) Passion fruit tissue culture medium and application thereof in rapid propagation of detoxified Passion fruit tissue culture seedlings
CN104938335A (en) Method for obtaining regeneration plants by use of camellia oleifera hypocotyl
CN110214694B (en) Tissue culture rapid propagation method of male and female plants of hemsleya amabilis
CN101743908A (en) Tissue culture, rapid propagation and cultivation method of grevillea banksii
CN100391333C (en) Aseptic seedling tissue culturing and test tube seedling hardening off and transplating technology for anthurium andraeanum
CN101015279B (en) Tissue culture method for fast propagation of primula poissonii
CN103299902B (en) Method for carrying out tissue culture on chinaberry seedlings
CN105409779A (en) Tissue culture rapid reproduction method for Cinnamomum kanehirae
CN108184462A (en) A kind of open country eggplant sapling stock breeding method and its method for grafting fruits and vegetables
CN1327760C (en) Flower-forcing method for fortunes paulownla seedling
CN114027182A (en) Tissue culture propagation method for dolichos succulent plants in crassulaceae echeveria

Legal Events

Date Code Title Description
A201 Request for examination
E902 Notification of reason for refusal
E701 Decision to grant or registration of patent right
GRNT Written decision to grant
FPAY Annual fee payment

Payment date: 20130305

Year of fee payment: 9

FPAY Annual fee payment

Payment date: 20140609

Year of fee payment: 10

FPAY Annual fee payment

Payment date: 20150421

Year of fee payment: 11

LAPS Lapse due to unpaid annual fee