CN108184462A - A kind of open country eggplant sapling stock breeding method and its method for grafting fruits and vegetables - Google Patents
A kind of open country eggplant sapling stock breeding method and its method for grafting fruits and vegetables Download PDFInfo
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- CN108184462A CN108184462A CN201810010860.1A CN201810010860A CN108184462A CN 108184462 A CN108184462 A CN 108184462A CN 201810010860 A CN201810010860 A CN 201810010860A CN 108184462 A CN108184462 A CN 108184462A
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- sapling
- stock
- eggplant
- vegetables
- culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Abstract
The invention discloses a kind of wild eggplant sapling stock breeding method and its methods for grafting fruits and vegetables.The breeding method of the open country eggplant sapling stock includes the explant for choosing wild eggplant tree, sterilizing, it is seeded on differential medium MS+BA3mg/L+NAA0.2mg/L+3% sucrose, Proliferation, Differentiation, which goes out after healthy and strong seedling to cut, to be inoculated on culture medium MS+IAA0.5mg/L or MS+NAA0.1mg/L, sealing culture, forms intact plant；Opening culture, domestication, hardening will can be used as stock after wild eggplant sapling transplant planting.The wild eggplant sapling that the present invention cultivates so that the disease resistance for grafting fruits and vegetables is strong as stock, prevent and reduce the soil-borne disease approach of grafting fruits and vegetables well, the growth service life of fruits and vegetables is grafted up to decades, the four seasons can bloom fruiting, cultivation management than traditional fruits and vegetables is extensive, has many advantages, such as that at low cost, quality is good, yield is high and benefit protrudes.
Description
Technical field
The present invention relates to agricultural plantation technology fields, are specifically related to a kind of wild eggplant sapling stock breeding method and its grafting
The method of fruits and vegetables.
Background technology
Grafting is one kind of vegetative reproduction in vegetative propagation.The forming layer of scion and stock should be made closely to tie during grafting
It closes, to ensure that scion survives.The branch or bud connected, is called scion；The plant connect is called stock or platform wood.During scion
The general seedling for selecting 2 to 4 buds of tool becomes the top or top of plant, becomes the root of plant after stock grafting after grafting
Pastern point.
Stock has wide influence to scion, such as Growth status and robustness, root physiology biochemical characteristic and its to environment
Adaptability etc.；Scion also has a significant effect to stock, as root growth ability, root regeneration ability, root density and
Root.Based on this, it is now recognized that the principal element for influencing grafting survival is the affinity of scion and stock, secondly the technology of grafting
With the management after grafting.Therefore, the strong stock of selective affinity is generally tended to, and stock cultivation is paid little attention to, it is practical
On, the cultivation of stock is to grafting the service life of species, growing, blossom and bear fruit and adaptability etc. all has a great impact.
Invention content
The present invention provides a kind of wild eggplant sapling stock breeding method and its grafts the side of fruits and vegetables by the cultivation of stock
Method improves the service life of grafting fruits and vegetables, grows, blossoms and bears fruit and adaptability.
The breeding method of open country eggplant sapling stock of the invention includes the following steps：
(1) explant of wild eggplant tree is taken, is sterilized；
(2) above-mentioned explant is cut into essentially identical size, such as 1 centimeter of length or 1 centimeter of square, is seeded in culture
On base A, sealing culture grows segment after endless full stand, is inoculated on culture medium B, and sealing culture, Proliferation, Differentiation goes out healthy and strong small
Seedling；
(3) seedling on culture medium B is cut and be inoculated into culture medium C, sealing culture, daily illumination for a period of time, is formed
Intact plant；
(4) open culture makes plant be removed after adapting to external environment, washes away root culture medium, is transplanted to and is planted rich in good
In seedbed or the hole tray of training matrix；
(5) after a period of time, wild eggplant sapling is transplanted and can be used as stock to after being colonized a period of time between field or ground.
Explant described in step (1) of the present invention can be one in cotyledon, stem apex, stem section, hypocotyl, petiole or blade
Kind is several.
Sterilizing described in step (1) of the present invention is to wash away explant surface dirt and fine hair with detergent, and flowing water rinses, so
Afterwards aseptic water washing is used with ethyl alcohol surface sterilization, 0.1% mercuric chloride solution soaking disinfection on superclean bench.
Step (1) can take wild eggplant leaf piece or petiole in the present invention, and surface dirt and fine hair are washed away with detergent, stream
Water rinses, then on superclean bench with 75% ethyl alcohol surface sterilization 8 to 12 minutes, 0.1% mercuric chloride solution soaking disinfection 5~
15 seconds, with aseptic water washing 3 to 8 times, 2 to 4 seconds/time.
The formula of culture medium A and culture medium B can be MS+BA3mg/L+NAA0.2mg/L+3% in step (2) of the present invention
Sucrose.
The formula of culture medium C can be MS+IAA0.5mg/L or MS+NAA0.1mg/L in step (3) of the present invention.
Culture medium described in step (2) of the present invention and step (3), sucrose is carbon source, agar solidified with 0.7%~0.8%,
PH is 5.8~6.0, adds auximone, and culture medium is sub-packed in tissue culture container, moist heat sterilization after sealing.
Above-mentioned moist heat sterilization temperature can be 100~135 DEG C, such as 120~125 DEG C, sterilization time is 25~35 minutes,
Such as 30 minutes.
In the present invention, the sealing culture is cultivated to be placed in culturing room, cultivates 22~28 DEG C of room temperature, intensity of illumination
1000 to 2000lx, 10~12 hour/day of light application time.
Cultivation matrix can be soil or turfy soil rich in organic matter in step (4) of the present invention.
In step (4) of the present invention before transplanting plant, to seedbed or hole tray suitably 800 times of Bravo sterilizings of sprinkling.
Seedbed or hole tray are covered into film after transplanting in step (4) of the present invention, are placed in cool canopy, temperature is maintained at 20~25
DEG C, film is thrown off after 5~15 days, and gradually increase intensity of illumination.
Planting survival rates are up to more than 95% in step (5) of the present invention.
The method of present invention grafting fruits and vegetables includes the following steps：
(1) field planting 20~28 months, the wild eggplant sapling stock of 0.7~1.3 centimetre of Miao Jing, by stock from from the ground are selected
80cm transversely cuttings, from stock center longitudinal cut；
(2) fruits and vegetables seed is chosen, seed soaking, sowing obtain fruits and vegetables seedling；
(3) it chooses fruits and vegetables terminal bud or lateral bud makees scion, be inserted into stock notch, plastics are pressed from both sides above or use with clip after being directed at cortex
Film tightens.
Preferably, the fruits and vegetables are one or more of eggplant, tomato and capsicum.
Compared with prior art, the disease resistance of fruits and vegetables is grafted as stock by the wild eggplant sapling that the present invention cultivates
By force, prevent and reduce the soil-borne disease approach of grafting fruits and vegetables well, and graft growth service life of fruits and vegetables up to decades,
The four seasons can bloom fruiting, and the cultivation management than traditional fruits and vegetables is extensive, have at low cost, quality is good, yield is high and benefit protrudes etc.
Advantage.
Specific embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.As long as in addition, technical characteristic involved in the various embodiments of the present invention described below each other it
Between do not form conflict and can be combined with each other.
Embodiment 1：The culture of intact plant
1. sterilizing methods
Wild eggplant leaf piece or petiole are taken, blocky or section shape is cut into, is put into enamel enema syringe, first washes away surface with washing powder
Dust and fine hair are bandaged with gauze on mouth, then are rinsed two hours with tap water flowing water.Then, incline except extra water, will locate in advance
The explant material of reason is transferred in the beaker of 1000ml, by 75% ethanol solution on the superclean bench crossed in purification aseptic process
It pours into beaker, liquid level covers explant surface, and after sterilizing 8 to 12 minutes, 0.1% is poured into after the clean ethanol solution that inclines as possible
Mercuric chloride solution soaking disinfection 10 seconds, incline clean mercuric chloride solution as possible, immediately with aseptic water washing 5 to 7 times, 50 to 60 seconds/
It is secondary.
2. condition of culture
The formula of culture medium A and culture medium B are MS+BA3mg/L+NAA0.2mg/L+3% sucrose, and the formula of culture medium C is
MS+IAA0.5mg/L or MS+NAA0.1mg/L.
In above-mentioned culture medium, sucrose is carbon source, with 0.7% it is agar solidified, adjust PH be 5.8~6.0, add plant life
Long element, by the amount packing culture medium of every bottle of 30ml or so in the glass tissue culture bottle of 500ml capacity, in 121 DEG C of conditions after sealing
Lower moist heat sterilization 30 minutes, material inoculation, which is placed in culturing room, cultivates, and cultivates 25~28 DEG C of room temperature, intensity of illumination
2000lx, 12 hour/day of light application time.
3. the induction of callus, the formation of endless full stand and proliferation
Blade is cut into 1 centimeter of square, petiole is cut into 1 centimeter long section, is seeded in culture medium A, by above-mentioned condition of culture
It is cultivated in culturing room.Explant thickens thicker after two weeks, the callus that edge of wound appearance is irregularly swelled, and color is gradual
It deepens.Inoculation generates many pores in callus and undamaged petiole surface after three weeks, and after being inoculated with four weeks, these are small
Stain grows up to thickly dotted endless full stand, and seedling color gradually becomes shallower as, after become blackish green or green, callus differentiation and seedling emergence
Rate is up to more than 95%.By the endless full stand after cultivating five weeks, such as blade, petiole, stem apex, stem section segment, it is inoculated into culture medium B
It is proliferated, differentiates a large amount of healthy and strong seedlings after 15 days, differentiation rate is up to more than 95%.
It is inoculated into culture medium C after long 3 centimeters or more endless full stands are cut, is that visible base portion grows many after ten days
System forms intact plant.
Embodiment 2：Domestication, hardening, grafting and transplanting
1. domestication and hardening
Before transplanting above-mentioned test tube seedling, first open wide bottleneck and tamed for 2 to 3 days, to adapt to external environment.It selects rich in organic
Matter soil or the whole compartment of turfy soil or dress hole tray, appropriate 800 times of Bravo sterilizings of sprinkling.During test tube seedling bottle outlet, attachment is carefully washed away
Culture medium in root tries not to injure root system.Carefully it is transplanted in the seedbed got ready or hole tray.Root water is watered with after cultivation,
Cool canopy is put into, covers film to keep humidity, and pay attention to ventilation.Hardening temperature is maintained at 20~25 DEG C, is thrown off after 10 days
Film, and intensity of illumination is gradually increased, the water of sprinkling in every 3 to 4 days (depending on temperature).Field is colonized in after one month, into
Motility rate is up to more than 95%.
2. grafting, transplanting
The wild eggplant sapling that tissue culture goes out is colonized 2 years in field, and Miao Jing is laterally cut at 1 centimeter, by stock from 80cm from the ground
It is disconnected, from the deep 1~2cm of stock center longitudinal incision.The terminal bud of eggplant or lateral bud to be made into scion, stay 3~4 leaves, lower end is cut into 0.8~
The knife spine Wedge insertion stock notch of 1cm is aligned after cortex and above or with plastic film is tightened with clip folder.No matter spring, summer, autumn and winter,
It transplants within 30 days after grafting, grows vigorous, basic no disease and pests harm, transplanting was fruiting of blooming after one month, and fruit colour is bright-coloured, single plant
Yield is per year over 200 jin.
Embodiment 3：The culture of intact plant
As different from Example 1：The formula of culture medium A be MS+BA2mg/L+ NAA0.2mg/L+3% sucrose, callus
The appearance of tissue is slower than embodiment 1, and explant callus differentiation and seedling emergence rate is 85%, Proliferation, Differentiation rate 85%, stock field planting
Survival rate 90% afterwards, about 160 jin of single plant eggplant average annual productivity.
Embodiment 4：The culture of intact plant
As different from Example 1：The formula of culture medium A be MS+BA1mg/L+ NAA0.3mg/L+3% sucrose, callus
The appearance of tissue is slower than embodiment 1, and explant callus differentiation and seedling emergence rate is 78%, Proliferation, Differentiation rate 75%, stock field planting
Survival rate 89% afterwards, about 160 jin of single plant eggplant average annual productivity.
Embodiment 5：The culture of intact plant
As different from Example 1：The formula of culture medium A is MS+BA0.5mg/L+ NAA0.2mg/L+IAA0.1mg/L+
3% sucrose, the appearance of callus is slower than embodiment 1, and explant callus differentiation and seedling emergence rate is 76%, Proliferation, Differentiation rate
76%, survival rate 85% after stock field planting, about 150 jin of single plant eggplant average annual productivity.
Embodiment 6：Domestication, hardening, grafting and transplanting
As different from Example 1：Eggplant, the terminal bud of capsicum and tomato these three fruits and vegetables or lateral bud are made into scion, stay 3~
The knife spine Wedge insertion stock notch of 0.8~1cm is cut into 4 leaves, lower end, and plastic film is pressed from both sides above or use with clip after being directed at cortex
It tightens.No matter spring, summer, autumn and winter, transplant within 30 days after grafting, grow vigorous, basic no disease and pests harm, transplanting was extension of blooming after one month
Fruit, fruit colour is bright-coloured, and single plant yield is per year over 200 jin.
As it will be easily appreciated by one skilled in the art that the foregoing is merely illustrative of the preferred embodiments of the present invention, not to
The limitation present invention, all any modification, equivalent and improvement made all within the spirits and principles of the present invention etc., should all include
Within protection scope of the present invention.
Claims (10)
1. a kind of open country eggplant sapling stock breeding method, which is characterized in that the breeding method includes the following steps：
(1) explant of wild eggplant tree is taken, is sterilized；
(2) above-mentioned explant is cut into essentially identical size, such as 1 centimeter of length or 1 centimeter of square, is seeded in culture medium A
On, sealing culture grows segment after endless full stand, is inoculated on culture medium B, and sealing culture, Proliferation, Differentiation goes out healthy and strong seedling；
(3) seedling on culture medium B is cut and be inoculated into culture medium C, sealing culture, daily illumination for a period of time, is formed complete
Plant；
(4) open culture makes plant be removed after adapting to external environment, washes away root culture medium, be transplanted to rich in good cultivation base
In the seedbed of matter or hole tray；
(5) after a period of time, wild eggplant sapling is transplanted and can be used as stock to after being colonized a period of time between field or ground.
2. open country eggplant sapling stock breeding method as described in claim 1, which is characterized in that explant is in the step (1)
One or more of cotyledon, stem apex, stem section, hypocotyl, petiole or blade.
3. open country eggplant sapling stock breeding method as described in claim 1, which is characterized in that sterilizing is to use in the step (1)
Detergent washes away explant surface dirt and fine hair, and flowing water rinses, and ethyl alcohol surface sterilization is then used on superclean bench,
0.1% mercuric chloride solution soaking disinfection with aseptic water washing, such as takes wild eggplant leaf piece or petiole, and surface ash is washed away with detergent
Dirt and fine hair, flowing water rinses, then with 75% ethyl alcohol surface sterilization 8 to 12 minutes, 0.1% mercuric chloride solution on superclean bench
Soaking disinfection 5~15 seconds, with aseptic water washing 3 to 8 times, 2 to 4 seconds/time.
4. open country eggplant sapling stock breeding method as described in claim 1, which is characterized in that in the step (2) culture medium A and
The formula of culture medium B is MS+BA3mg/L+NAA0.2mg/L+3% sucrose.
5. open country eggplant sapling stock breeding method as described in claim 1, which is characterized in that culture medium C in the step (3)
It is formulated as MS+IAA0.5mg/L or MS+NAA0.1mg/L.
6. open country eggplant sapling stock breeding method as described in claim 1, which is characterized in that the step (2) and step (3) training
It supports in base, sucrose is carbon source, agar solidified with 0.7%~0.8%, and PH is 5.8~6.0, adds auximone, culture medium point
Loaded in tissue culture container, moist heat sterilization after sealing, it is preferred that moist heat sterilization temperature is 100~135 DEG C, such as 120~125 DEG C,
Sterilization time is 25~35 minutes, such as 30 minutes.
7. open country eggplant sapling stock breeding method as described in claim 1, which is characterized in that the sealing culture is is placed in culture
22~28 DEG C of room temperature, intensity of illumination 1000 to 2000lx, 10~12 hour/day of light application time are cultivated in indoor culture.
8. open country eggplant sapling stock breeding method as described in claim 1, which is characterized in that cultivation matrix in the step (4)
To be rich in the soil or turfy soil of organic matter, it is preferred that before transplanting plant, to seedbed or hole tray suitably 800 times of Bravos of sprinkling
Sterilizing, it is preferred that seedbed or hole tray are covered into film after transplanting, are placed in cool canopy, temperature is maintained at 20~25 DEG C, 5~15 days
After throw off film, and gradually increase intensity of illumination.
9. open country eggplant sapling stock breeding method as described in claim 1, which is characterized in that field planting survives in the step (5)
Rate is up to more than 95%.
10. the method for grafting fruits and vegetables as described in any one of claim 1 to 9, which is characterized in that include the following steps：
(1) field planting 20~28 months is selected, the wild eggplant sapling stock of 0.7~1.3 centimetre of Miao Jing is horizontal from 80cm from the ground by stock
To cut-out, from stock center longitudinal cut；
(2) fruits and vegetables seed is chosen, is soaked seed, sowing obtains fruits and vegetables seedling；
(3) it chooses fruits and vegetables terminal bud or lateral bud makees scion, be inserted into stock notch, plastic film is pressed from both sides above or use with clip after being directed at cortex
It tightens.
Preferably, the fruits and vegetables are one or more of eggplant, tomato and capsicum.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810010860.1A CN108184462A (en) | 2018-01-05 | 2018-01-05 | A kind of open country eggplant sapling stock breeding method and its method for grafting fruits and vegetables |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810010860.1A CN108184462A (en) | 2018-01-05 | 2018-01-05 | A kind of open country eggplant sapling stock breeding method and its method for grafting fruits and vegetables |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111213504A (en) * | 2019-04-11 | 2020-06-02 | 浙江大学 | Woody stock for efficient grafting of solanaceous vegetables and efficient grafting seedling method thereof |
| CN112273077A (en) * | 2020-10-15 | 2021-01-29 | 湖北省农业科学院经济作物研究所 | Living body preservation method for solanum vegetable germplasm resources |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102017855A (en) * | 2009-09-11 | 2011-04-20 | 赖众民 | Method for grafting solanum torvum with vegetable eggplants |
| CN106508390A (en) * | 2016-10-28 | 2017-03-22 | 邓凯 | Rootstock cultivating method and plant grafting method |
| CN107494262A (en) * | 2017-08-28 | 2017-12-22 | 山东省果树研究所 | Tissue culture and rapid propagation method and application and culture medium and preparation method, the disinfection treatment method of apple rootstock bar for apple rootstock |
-
2018
- 2018-01-05 CN CN201810010860.1A patent/CN108184462A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102017855A (en) * | 2009-09-11 | 2011-04-20 | 赖众民 | Method for grafting solanum torvum with vegetable eggplants |
| CN106508390A (en) * | 2016-10-28 | 2017-03-22 | 邓凯 | Rootstock cultivating method and plant grafting method |
| CN107494262A (en) * | 2017-08-28 | 2017-12-22 | 山东省果树研究所 | Tissue culture and rapid propagation method and application and culture medium and preparation method, the disinfection treatment method of apple rootstock bar for apple rootstock |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111213504A (en) * | 2019-04-11 | 2020-06-02 | 浙江大学 | Woody stock for efficient grafting of solanaceous vegetables and efficient grafting seedling method thereof |
| WO2020206915A1 (en) * | 2019-04-11 | 2020-10-15 | 浙江大学 | Woody rootstock for effective grafting of solanaceous fruits and vegetables and effective grafting and seedling raising method therefor |
| CN112273077A (en) * | 2020-10-15 | 2021-01-29 | 湖北省农业科学院经济作物研究所 | Living body preservation method for solanum vegetable germplasm resources |
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