CN103843665B - A kind of Nandina domestica'Fire power' tissue culture and rapid propagation method based on LED light source - Google Patents
A kind of Nandina domestica'Fire power' tissue culture and rapid propagation method based on LED light source Download PDFInfo
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Abstract
The present invention relates to the tissue culture and rapid propagation method of a kind of Nandina domestica'Fire power' based on LED light source, belong to reproduction technique field.The method uses the LED light source step-by-step processing stage by stage of different light medium, monochromatic green glow is first used to train light source as Nandina domestica'Fire power' group, increase cane length and the interval of Nandina domestica'Fire power', then use the method for stem section propagation to breed, re-use daylight lamp and carry out stem section culture as light source.Compared with prior art, the proliferation times of Nandina domestica'Fire power' is brought up to 78 times by the present invention, growth cycle is shortened to 30 40d, solve Nandina domestica'Fire power' tissue cultured seedling growth cycle long, the present situation that proliferation times is low, on producing, save cost, drastically increased the quality of Nandina domestica'Fire power' reproduction speed and nursery stock, provide a kind of new method for Nandina domestica'Fire power' industrialized development offer guarantee for improving Nandina domestica'Fire power' reproduction speed.
Description
Technical field
The present invention relates to a kind of ornamental plant tissue culture method, especially relate to a kind of Nandina domestica'Fire power' based on LED light source
Tissue culture and rapid propagation method, belongs to reproduction technique field.
Background technology
Nandina domestica'Fire power', Shu little Ju section Nandina.Plant is short and small, and strain shape is compact, and blade is oval or avette, its internode
Ultrashort, the l/10 of only common Fructus Nandinae Domesticae, Nandina domestica'Fire power' starts rudiment in District of Shanghai, grow light red young leaves mid-April,
Growing up with blade, temperature raises, and more than 30 DEG C the most gradually turn green, December, when temperature drops to less than 5 DEG C, and day and night temperature exists
More than 10 DEG C, blade starts to redden, and owing to it resists cold evergreen, does not withers through the winter, the most beautiful, suitably do quilt and garth ring
Border is arranged.The research of Nandina domestica'Fire power' tissue culture technology, from the beginning of, mainly has the research of Nandina domestica'Fire power' Technique on Cuttage Propagation；
The research of Nandina domestica'Fire power' division propagation technology；The research etc. of Nandina domestica'Fire power' tissue culture method.Owing to Nandina domestica'Fire power' stipes is short,
Branch is few, uses traditional cuttage and plant division mode to breed, and speed is too slow, is hardly formed industrialized development, at present to fire
The research of flame Fructus Nandinae Domesticae tissue culture technology the most just starts, and the difficult problems such as the breeding cycle is long, and breeding coefficient is low also do not overcome, and cause flame
Fructus Nandinae Domesticae reproduction speed is slow, and development speed relatively lags behind, and has had a strong impact on the industrialized development of Nandina domestica'Fire power'.
Summary of the invention
The invention aims to overcome existing technological deficiency to provide the fire that a kind of breeding cycle is short, breeding coefficient is high
Flame Fructus Nandinae Domesticae quick breeding method for tissue culture.
For reaching above-mentioned purpose, the present invention is achieved by the following technical solutions:
A kind of tissue culture and rapid propagation method of Nandina domestica'Fire power' based on LED light source, the method comprises the following steps:
(1) Nandina domestica'Fire power' cane is obtained；
(2) the Nandina domestica'Fire power' stem section enrichment culture stage；
(3) the strong plantlets and rootage stage；
(4) the rooting culture stage；
It is characterized in that, during step (1) obtains Nandina domestica'Fire power' cane, utilize LED to train as Nandina domestica'Fire power' group
The most numerous original manual light source.
In above-mentioned steps (1), described LED is monochromatic green glow.
Further, specifically comprising the following steps that of step (1) takes Nandina domestica'Fire power' tissue cultured seedling, is inoculated into by the lateral bud separated
On subculture medium, being placed under green LED lamp cultivation, light application time is 12h/d；After cultivating 30-40d, each lateral bud divides
Dissolve 4-5 new lateral bud, and Nandina domestica'Fire power' lateral bud stem segment elongation is to 4-5cm, obtains described Nandina domestica'Fire power' cane.
As the further improvement of step of the present invention (1), described LED dominant wavelength is 525nm, and spectral range is
577-492nm, range of light intensity is 40-70 μm ol/m2·s。
Above-mentioned steps (2) specifically comprise the following steps that step (2) specifically comprise the following steps that employing plant division propagation and stem section
The method that propagation combines carries out subculture multiplication, and the cane obtained after cultivating in step (1) is cut into the little stem of 1cm-1.5cm
Section, this little stem section, at least provided with a blade, is then inoculated in the culture bottle equipped with adventitious bud proliferation culture medium by each stem section
Carry out enrichment culture, need the new lateral bud taken root to be placed in rejuvenation under common daylight lamp and cultivate, give illumination every day 16h, need to continue
The new lateral bud in generation continues to cultivate under LED, and proliferation times reaches 7-8 times.
Specifically comprising the following steps that of above-mentioned steps (3) will carry out, through daylight lamp, the flame that rejuvenation is cultivated in step (2)
Fructus Nandinae Domesticae is inoculated in Rooting and hardening-off culture base, is placed under common daylight lamp and carries out root culture, and light application time is 12h/d.
Specifically comprising the following steps that when the root length obtained after root culture in step (3) is 2-4cm of above-mentioned steps (4)
Time, carrying out rooting culture, in culture bottle moves to booth, seedling exercising is after 7-10 days, washes away culture medium, and root soaks mass concentration
Be 1% potassium permanganate solution carry out disinfection, then carry out outdoor transplanting.
The invention has the beneficial effects as follows: compared with prior art, use Nandina domestica'Fire power' tissue-culturing rapid propagation side provided by the present invention
Method, brings up to 7-8 times by breeding coefficient, will shorten to 30-40d proliferating cycle, and the root system of plant is sturdy, young sprout growing way is good,
Stem stalk is sturdy；Also can be cost-effective on producing, drastically increase the quality of Nandina domestica'Fire power' reproduction speed and nursery stock.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention made concrete introduction.
In the present invention, the formula of adventitious bud proliferation culture medium is: WPM culture medium and MS culture medium equal-volume mixed liquor,
Add 2.5mg/L6-BA, 7g/L agar and 30g/L sucrose, pH5.6.
Rooting and hardening-off culture based formulas is: WPM+0.5mg/LIBA+7g/L agar+30g/L sucrose, pH5.6.
Embodiment 1
A kind of Nandina domestica'Fire power' tissue culture and rapid propagation method based on LED light source, comprises the following steps:
(1) Nandina domestica'Fire power' cane is obtained
Take Nandina domestica'Fire power' tissue cultured seedling, the lateral bud separated is inoculated on subculture medium, be placed under green LED lamp cultivation, illumination
Time is 12h/d, and light intensity is 40 μm ol/m2·s；After cultivating 30d, each lateral bud redifferentiation goes out 4 new lateral buds, and flame
Fructus Nandinae Domesticae lateral bud stem segment elongation is to 4.2cm；Wherein, green LED lamp dominant wavelength is 525nm, and spectral range is 577-492nm.
(2) the Nandina domestica'Fire power' stem section enrichment culture stage
The method using plant division propagation and stem section propagation to combine carries out subculture multiplication, and cane is cut into 0.5cm stem section, each stem section band
There is a lateral bud, be inoculated in adventitious bud proliferation culture medium and carry out enrichment culture, need take root to be placed in rejuvenation under common daylight lamp
Cultivating, give illumination every day 16h/d, need the new lateral bud of subculture to continue under green LED lamp and cultivate, proliferation times reaches
7.0 again；
(3) the strong plantlets and rootage stage
The Nandina domestica'Fire power' carrying out rejuvenation cultivation through daylight lamp is inoculated in Rooting and hardening-off culture base, is placed under common daylight lamp
Row root culture, light application time is 16h/d；
(4) the rooting culture stage
When root length is 3cm, carrying out rooting culture, culture bottle moves to seedling exercising 7 days in booth, washes away culture medium, root soaks
Mass concentration is that 1% potassium permanganate solution carries out disinfection, and then carries out outdoor transplanting.
Use said method so that Nandina domestica'Fire power' is as short as 40 days proliferating cycle, and breeding coefficient is up to 7 times.
Embodiment 2
A kind of Nandina domestica'Fire power' tissue culture and rapid propagation method based on LED light source, it is characterised in that comprise the following steps:
(1) Nandina domestica'Fire power' cane is obtained
Take Nandina domestica'Fire power' tissue cultured seedling, the lateral bud separated is inoculated on subculture medium, be placed under green LED lamp cultivation, illumination
Time is 12h/d, and light intensity is 50 μm ol/m2·s；After cultivating 35d, each lateral bud redifferentiation goes out 5 new lateral buds, and flame
Fructus Nandinae Domesticae lateral bud stem segment elongation is to 4.8cm；
(2) the Nandina domestica'Fire power' stem section enrichment culture stage
The method using plant division propagation and stem section propagation to combine carries out subculture multiplication, and cane is cut into 1cm stem section, each stem section band
There is a lateral bud, be inoculated in adventitious bud proliferation culture medium and carry out enrichment culture, need take root to be placed in rejuvenation under common daylight lamp
Cultivating, give illumination every day 16h/d, need the new lateral bud of subculture to continue under green LED lamp and cultivate, proliferation times reaches
7.8 again；
(3) the strong plantlets and rootage stage
The Nandina domestica'Fire power' carrying out rejuvenation cultivation through daylight lamp is inoculated in Rooting and hardening-off culture base, is placed under common daylight lamp
Row root culture, light application time is 16h/d；
(4) the rooting culture stage
When root length is 3cm, carrying out rooting culture, culture bottle moves to seedling exercising 7 days in booth, washes away culture medium, root soaks
Mass concentration is that 1% potassium permanganate solution carries out disinfection, and then carries out outdoor transplanting.
Use said method so that Nandina domestica'Fire power' is as short as 34 days proliferating cycle, and breeding coefficient is up to 7.8 times.
Embodiment 3
A kind of Nandina domestica'Fire power' tissue culture and rapid propagation method based on LED light source, it is characterised in that comprise the following steps:
(1) Nandina domestica'Fire power' cane is obtained
Take Nandina domestica'Fire power' tissue cultured seedling, the lateral bud separated is inoculated on subculture medium, be placed under green LED lamp cultivation, illumination
Time is 12h/d, and light intensity is 70 μm ol/m2·s；After cultivating 40d, each lateral bud redifferentiation goes out 5 new lateral buds, and flame
Fructus Nandinae Domesticae lateral bud stem segment elongation is to 5cm；
(2) the Nandina domestica'Fire power' stem section enrichment culture stage
The method using plant division propagation and stem section propagation to combine carries out subculture multiplication, and cane is cut into 0.8cm stem section, each stem section band
There is a lateral bud, be inoculated in adventitious bud proliferation culture medium and carry out enrichment culture, need take root to be placed in rejuvenation under common daylight lamp
Cultivating, give illumination every day 16h/d, need the new lateral bud of subculture to continue under green LED lamp and cultivate, proliferation times reaches
7.8 again；
(3) the strong plantlets and rootage stage
The Nandina domestica'Fire power' carrying out rejuvenation cultivation through daylight lamp is inoculated in Rooting and hardening-off culture base, is placed under common daylight lamp
Row root culture, light application time is 16h/d；
(4) the rooting culture stage
When root length is 3cm, carrying out rooting culture, culture bottle moves to seedling exercising 10 days in booth, washes away culture medium, root soaks
Mass concentration is that 1% potassium permanganate solution carries out disinfection, and then carries out outdoor transplanting.
Use said method so that Nandina domestica'Fire power' is as short as 30 days proliferating cycle, and breeding coefficient is up to 7.8 times.
Claims (4)
1. a tissue culture and rapid propagation method for Nandina domestica'Fire power' based on LED light source, the method comprises the following steps:
(1) Nandina domestica'Fire power' cane is obtained；
(2) the Nandina domestica'Fire power' stem section enrichment culture stage；
(3) the strong plantlets and rootage stage；
(4) the rooting culture stage；
It is characterized in that, utilize LED as Nandina domestica'Fire power' tissue-culturing rapid propagation in step (1) obtains Nandina domestica'Fire power' cane
Original manual light source；Wherein, described LED is monochromatic green glow, and described LED dominant wavelength is 525nm, wave spectrum model
Enclosing for 577-492nm, range of light intensity is 40-70 μm ol/m2 s；
Specifically comprising the following steps that of step (1) takes Nandina domestica'Fire power' tissue cultured seedling, is inoculated on subculture medium by the lateral bud separated, puts
Cultivating under green LED lamp, light application time is 12h/d；After cultivating 30-40d, each lateral bud redifferentiation goes out 4-5 newly
Lateral bud, and Nandina domestica'Fire power' lateral bud stem segment elongation is to 4-5cm, obtains described Nandina domestica'Fire power' cane；
Specifically comprising the following steps that of step (2) uses plant division propagation and the stem section method that combines of propagation to carry out subculture multiplication, by step
(1) cane obtained after cultivating in is cut into 0.5cm-1.0cm little stem section, and each stem section is at least provided with a blade, then
This little stem section is inoculated in the culture bottle equipped with adventitious bud proliferation culture medium and carries out enrichment culture, need the new lateral bud taken root to put
Under common daylight lamp, rejuvenation is cultivated, and gives illumination every day 16h, needs the new lateral bud of subculture to continue under LED and cultivates,
Proliferation times reaches 7-8 times；
The formula of above-mentioned adventitious bud proliferation culture medium is: WPM culture medium and MS culture medium equal-volume mixed liquor, adds 2.5mg/
L6-BA, 7g/L agar and 30g/L sucrose, pH5.6.
The tissue culture and rapid propagation method of Nandina domestica'Fire power' based on LED light source the most according to claim 1, it is characterised in that: step
Suddenly (3) specifically comprise the following steps that by step (2) through daylight lamp carried out rejuvenation cultivate Nandina domestica'Fire power' be inoculated in
In Rooting and hardening-off culture base, being placed under common daylight lamp and carry out root culture, light application time is 12h/d.
The tissue culture and rapid propagation method of Nandina domestica'Fire power' based on LED light source the most according to claim 2, it is characterised in that:
Rooting and hardening-off culture based formulas is: WPM+0.5mg/LIBA+7g/L agar+30g/L sucrose, pH5.6.
The tissue culture and rapid propagation method of Nandina domestica'Fire power' based on LED light source the most according to claim 3, it is characterised in that:
Specifically comprising the following steps that when the root length obtained after root culture in step (3) is 2-4cm of step (4), is carried out
Rooting culture, in culture bottle moves to booth, seedling exercising is after 7-10 days, washes away culture medium, and it is 1% that root soaks mass concentration
Potassium permanganate solution carry out disinfection, then carry out outdoor transplanting.
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| CN104982196B (en) * | 2015-06-26 | 2018-05-11 | 江苏绿苑园林建设有限公司 | A kind of breeding method for accelerating the Nandina domestica'Fire power' speed of growth |
| CN108124683A (en) * | 2017-12-29 | 2018-06-08 | 佛山市三水阳特园艺有限公司 | A kind of signature seeds seedling production method |
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| CN101044841A (en) * | 2006-12-22 | 2007-10-03 | 上海市农业科学院 | Fast breeding system for flame nandina |
| CN102138526A (en) * | 2011-03-24 | 2011-08-03 | 浙江森禾种业股份有限公司 | Tissue culture method of red-leaf sacred bamboo |
| CN102939843A (en) * | 2012-11-26 | 2013-02-27 | 江苏绿苑园林建设有限公司 | Method for domesticating hardening seedlings of tissue culture seedlings of Nandina domestica firepower |
| CN103314853A (en) * | 2013-06-28 | 2013-09-25 | 江苏农林职业技术学院 | Tissue culture method for flame nandina |
| CN103430839A (en) * | 2013-08-01 | 2013-12-11 | 浙江农林大学 | Culture method of ellcalyplus grandis Hill ex Maid tissue culture seedling |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102273403B (en) * | 2010-06-08 | 2013-03-06 | 上海上房园艺有限公司 | Tissue culture method of nandina domestica firepower |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101044841A (en) * | 2006-12-22 | 2007-10-03 | 上海市农业科学院 | Fast breeding system for flame nandina |
| CN102138526A (en) * | 2011-03-24 | 2011-08-03 | 浙江森禾种业股份有限公司 | Tissue culture method of red-leaf sacred bamboo |
| CN102939843A (en) * | 2012-11-26 | 2013-02-27 | 江苏绿苑园林建设有限公司 | Method for domesticating hardening seedlings of tissue culture seedlings of Nandina domestica firepower |
| CN103314853A (en) * | 2013-06-28 | 2013-09-25 | 江苏农林职业技术学院 | Tissue culture method for flame nandina |
| CN103430839A (en) * | 2013-08-01 | 2013-12-11 | 浙江农林大学 | Culture method of ellcalyplus grandis Hill ex Maid tissue culture seedling |
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