CN103843665A - Nandina domestica tissue culture and rapid propagation method based on LED (light-emitting diode) light sources - Google Patents
Nandina domestica tissue culture and rapid propagation method based on LED (light-emitting diode) light sources Download PDFInfo
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- CN103843665A CN103843665A CN201410112856.8A CN201410112856A CN103843665A CN 103843665 A CN103843665 A CN 103843665A CN 201410112856 A CN201410112856 A CN 201410112856A CN 103843665 A CN103843665 A CN 103843665A
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- 240000007695 Nandina domestica Species 0.000 title claims abstract description 68
- 238000000034 method Methods 0.000 title claims abstract description 36
- 239000002609 medium Substances 0.000 claims description 14
- 230000035755 proliferation Effects 0.000 claims description 12
- 230000003716 rejuvenation Effects 0.000 claims description 10
- 241000196324 Embryophyta Species 0.000 claims description 8
- 230000003203 everyday effect Effects 0.000 claims description 5
- 238000005286 illumination Methods 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 5
- 239000012879 subculture medium Substances 0.000 claims description 5
- 229920001817 Agar Polymers 0.000 claims description 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 4
- 229930006000 Sucrose Natural products 0.000 claims description 4
- 239000008272 agar Substances 0.000 claims description 4
- 239000005720 sucrose Substances 0.000 claims description 4
- 238000001228 spectrum Methods 0.000 claims description 3
- 238000012136 culture method Methods 0.000 claims description 2
- 238000012258 culturing Methods 0.000 claims description 2
- 239000006870 ms-medium Substances 0.000 claims description 2
- 230000000644 propagated effect Effects 0.000 abstract 1
- 238000005516 engineering process Methods 0.000 description 4
- 230000002062 proliferating effect Effects 0.000 description 4
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 2
- 241000133556 Nandina Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
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Abstract
The invention relates to a Nandina domestica tissue culture and rapid propagation method based on LED (light-emitting diode) light sources, and belongs to the technical field of propagation. The method is characterized in that the LED light sources with different light qualities are adopted for treatment in stages step by step, single-color green is firstly used as a Nandina domestica tissue culture light source to increase stem lengths and spacing between the stems of the Nandina domestica, then propagation is performed by adopting a stem propagation method, and a fluorescent lamp is used as a light source for stem culture. Compared with the prior art, the Nandina domestica tissue culture and rapid propagation method has the advantages that the Nandina domestica is propagated to 7-8 times, the growth period is shortened to 30-40 days, the situation that the Nandina domestica tissue culture growth cycle is long and the propagation rate is low is solved, the production cost is lowered, the propagation speed of Nandina domestica and the quality of seedlings are greatly increased, and a new method is provided for increasing the propagation speed of the Nandina domestica so as to provide guarantee for industrialization development of the Nandina domestica.
Description
Technical field
The present invention relates to a kind of ornamental plant tissue culture method, especially relate to the tissue culture and rapid propagation method of a kind of flame heavenly bamboo based on LED light source, belong to propagation technique field.
Background technology
Flame heavenly bamboo, Shu little Ju section Nandina.Plant is short and small, strain shape compactness, and blade is oval or avette, its internode is ultrashort, only has the l/10 of common heavenly bamboo, and flame heavenly bamboo starts rudiment mid-April in District of Shanghai, grow light red young leaves, grow up with blade, temperature raises, 30 DEG C just turn green above gradually, and December, when temperature drops to below 5 DEG C, day and night temperature is more than 10 DEG C, and blade starts to redden, because it is cold-resistant evergreen, do not wither through the winter, very beautiful, suitable do quilt and garth environment arrange.The research of flame heavenly bamboo tissue culture technology from recent years just, mainly contain the research of flame heavenly bamboo Technique on Cuttage Propagation; The research of flame heavenly bamboo division propagation technology; The research of flame heavenly bamboo tissue culture method etc.Because flame heavenly bamboo stipes is short, branch is few, adopt traditional cuttage and plant division mode to breed, speed is too slow, is difficult to form industrialized development, at present the research of flame heavenly bamboo tissue culture technology is just just started, the difficult problems such as the breeding cycle is long, and reproduction coefficient is low also do not overcome, and cause flame heavenly bamboo reproduction speed slow, development speed relatively lags behind, and has had a strong impact on the industrialized development of flame heavenly bamboo.
Summary of the invention
The object of the invention is provides the flame heavenly bamboo that a kind of breeding cycle is short, reproduction coefficient is high quick breeding method for tissue culture in order to overcome existing technological deficiency.
For achieving the above object, the present invention is achieved by the following technical solutions:
A tissue culture and rapid propagation method for flame heavenly bamboo based on LED light source, the method comprises the following steps:
(1) obtain flame heavenly bamboo cane;
(2) flame Nan Tian culm section propagation cultivation stage;
(3) the strong plantlets and rootage stage;
(4) the rooting culture stage;
It is characterized in that, obtain and in flame heavenly bamboo cane, utilize the original manual light source of LED lamp as flame heavenly bamboo tissue-culturing rapid propagation in step (1).
In above-mentioned steps (1), described LED lamp is monochromatic green glow.
Further, the concrete steps of step (1) are as follows: the flame heavenly bamboo of getting fire group training seedling, the lateral bud separating is inoculated on subculture medium, and be placed under green LED lamp and cultivate, light application time is 12h/d; Cultivate after 30-40d, each lateral bud redifferentiation goes out 4-5 new lateral bud, and flame heavenly bamboo lateral bud stem segment elongation is to 4-5cm, obtains described flame heavenly bamboo cane.
As the further improvement of step of the present invention (1), described LED lamp dominant wavelength is 525nm, and wave spectrum scope is 577-492nm, and range of light intensity is 40-70 μ mol/m
2s.
The concrete steps of above-mentioned steps (2) are as follows: the concrete steps of step (2) are as follows: adopt plant division propagation and stem section to breed the method combining and carry out shoot proliferation, the cane obtaining after cultivating in step (1) is cut into the little stem section of 1cm-1.5cm, each stem section is at least with a blade, then this little stem section is inoculated in and in the blake bottle that adventitious bud proliferation medium is housed, breeds cultivation, the new lateral bud that need to take root is placed in rejuvenation under common fluorescent lamp and cultivates, give illumination every day 16h, need the new lateral bud of subculture to continue to cultivate under LED lamp, propagation multiple reaches 7-8 doubly.
The concrete steps of above-mentioned steps (3) are as follows: the flame heavenly bamboo that process fluorescent lamp in step (2) was carried out to rejuvenation cultivation is inoculated in Rooting and hardening-off culture base, is placed under common fluorescent lamp and carries out culture of rootage, and light application time is 12h/d.
The concrete steps of above-mentioned steps (4) are as follows: in the time that the root length obtaining after culture of rootage in step (3) is 2-4cm, carry out rooting culture, blake bottle was moved in booth after hardening 7-10 days, wash away medium, root soaks the liquor potassic permanganate that mass concentration is 1% and carries out disinfection, and then carries out outdoor transplanting.
The invention has the beneficial effects as follows: compared with prior art, adopt flame heavenly bamboo tissue culture and rapid propagation method provided by the present invention, reproduction coefficient is brought up to 7-8 doubly, will shorten to 30-40d proliferating cycle, and the root system of plant is sturdy, young sprout growing way is good, stem stalk is sturdy; Also can be cost-saving on producing, greatly improve the quality of flame heavenly bamboo reproduction speed and nursery stock.
Embodiment
Below in conjunction with specific embodiment, the present invention is done to concrete introduction.
In the present invention, the formula of adventitious bud proliferation medium is: WPM medium and MS medium equal-volume mixed liquor, add 2.5mg/L6-BA, 7g/L agar and 30g/L sucrose, pH5.6.
Rooting and hardening-off culture based formulas is: WPM+0.5mg/LIBA+7g/L agar+30g/L sucrose, pH5.6.
embodiment 1
A flame heavenly bamboo tissue culture and rapid propagation method based on LED light source, comprises the following steps:
(1) obtain flame heavenly bamboo cane
The flame heavenly bamboo of getting fire group training seedling, is inoculated into the lateral bud separating on subculture medium, is placed under green LED lamp and cultivates, and light application time is 12h/d, and light intensity is 40 μ mol/m
2s; Cultivate after 30d, each lateral bud redifferentiation goes out 4 new lateral buds, and flame heavenly bamboo lateral bud stem segment elongation is to 4.2cm; Wherein, green LED lamp dominant wavelength is 525nm, and wave spectrum scope is 577-492nm.
(2) flame Nan Tian culm section propagation cultivation stage
Adopt plant division propagation and stem section to breed the method combining and carry out shoot proliferation, cane is cut into 0.5cm stem section, each stem section is with a lateral bud, be inoculated in and on adventitious bud proliferation medium, breed cultivation, the rejuvenation under common fluorescent lamp that is placed in that need to take root is cultivated, give illumination every day 16h/d, need the new lateral bud of subculture to continue to cultivate under green LED lamp, propagation multiple reaches 7.0 times;
(3) the strong plantlets and rootage stage
The flame heavenly bamboo that carried out rejuvenation cultivation through fluorescent lamp is inoculated in Rooting and hardening-off culture base, is placed under common fluorescent lamp and carries out culture of rootage, light application time is 16h/d;
(4) the rooting culture stage
Long during for 3cm when root, carry out rooting culture, blake bottle is moved in booth to hardening 7 days, wash away medium, it is that 1% liquor potassic permanganate carries out disinfection that root soaks mass concentration, then carries out outdoor transplanting.
Adopt said method, make flame heavenly bamboo be as short as 40 days proliferating cycle, reproduction coefficient is up to 7 times.
embodiment 2
A flame heavenly bamboo tissue culture and rapid propagation method based on LED light source, is characterized in that, comprises the following steps:
(1) obtain flame heavenly bamboo cane
The flame heavenly bamboo of getting fire group training seedling, is inoculated into the lateral bud separating on subculture medium, is placed under green LED lamp and cultivates, and light application time is 12h/d, and light intensity is 50 μ mol/m
2s; Cultivate after 35d, each lateral bud redifferentiation goes out 5 new lateral buds, and flame heavenly bamboo lateral bud stem segment elongation is to 4.8cm;
(2) flame Nan Tian culm section propagation cultivation stage
Adopt plant division propagation and stem section to breed the method combining and carry out shoot proliferation, cane is cut into 1cm stem section, each stem section is with a lateral bud, be inoculated in and on adventitious bud proliferation medium, breed cultivation, the rejuvenation under common fluorescent lamp that is placed in that need to take root is cultivated, give illumination every day 16h/d, need the new lateral bud of subculture to continue to cultivate under green LED lamp, propagation multiple reaches 7.8 times;
(3) the strong plantlets and rootage stage
The flame heavenly bamboo that carried out rejuvenation cultivation through fluorescent lamp is inoculated in Rooting and hardening-off culture base, is placed under common fluorescent lamp and carries out culture of rootage, light application time is 16h/d;
(4) the rooting culture stage
Long during for 3cm when root, carry out rooting culture, blake bottle is moved in booth to hardening 7 days, wash away medium, it is that 1% liquor potassic permanganate carries out disinfection that root soaks mass concentration, then carries out outdoor transplanting.
Adopt said method, make flame heavenly bamboo be as short as 34 days proliferating cycle, reproduction coefficient is up to 7.8 times.
embodiment 3
A flame heavenly bamboo tissue culture and rapid propagation method based on LED light source, is characterized in that, comprises the following steps:
(1) obtain flame heavenly bamboo cane
The flame heavenly bamboo of getting fire group training seedling, is inoculated into the lateral bud separating on subculture medium, is placed under green LED lamp and cultivates, and light application time is 12h/d, and light intensity is 70 μ mol/m
2s; Cultivate after 40d, each lateral bud redifferentiation goes out 5 new lateral buds, and flame heavenly bamboo lateral bud stem segment elongation is to 5cm;
(2) flame Nan Tian culm section propagation cultivation stage
Adopt plant division propagation and stem section to breed the method combining and carry out shoot proliferation, cane is cut into 0.8cm stem section, each stem section is with a lateral bud, be inoculated in and on adventitious bud proliferation medium, breed cultivation, the rejuvenation under common fluorescent lamp that is placed in that need to take root is cultivated, give illumination every day 16h/d, need the new lateral bud of subculture to continue to cultivate under green LED lamp, propagation multiple reaches 7.8 times;
(3) the strong plantlets and rootage stage
The flame heavenly bamboo that carried out rejuvenation cultivation through fluorescent lamp is inoculated in Rooting and hardening-off culture base, is placed under common fluorescent lamp and carries out culture of rootage, light application time is 16h/d;
(4) the rooting culture stage
Long during for 3cm when root, carry out rooting culture, blake bottle is moved in booth to hardening 10 days, wash away medium, it is that 1% liquor potassic permanganate carries out disinfection that root soaks mass concentration, then carries out outdoor transplanting.
Adopt said method, make flame heavenly bamboo be as short as 30 days proliferating cycle, reproduction coefficient is up to 7.8 times.
Claims (9)
1. a tissue culture and rapid propagation method for the flame heavenly bamboo based on LED light source, the method comprises the following steps:
(1) obtain flame heavenly bamboo cane;
(2) flame Nan Tian culm section propagation cultivation stage;
(3) the strong plantlets and rootage stage;
(4) the rooting culture stage;
It is characterized in that, obtain and in flame heavenly bamboo cane, utilize the original manual light source of LED lamp as flame heavenly bamboo tissue-culturing rapid propagation in step (1).
2. the tissue culture and rapid propagation method of the flame heavenly bamboo based on LED light source according to claim 1, is characterized in that, the LED lamp described in step (1) is monochromatic green glow.
3. the tissue culture and rapid propagation method of the flame heavenly bamboo based on LED light source according to claim 2, it is characterized in that: the concrete steps of step (1) are as follows: the flame heavenly bamboo of getting fire group training seedling, the lateral bud separating is inoculated on subculture medium, be placed under green LED lamp and cultivate, light application time is 12h/d; Cultivate after 30-40d, each lateral bud redifferentiation goes out 4-5 new lateral bud, and flame heavenly bamboo lateral bud stem segment elongation is to 4-5cm, obtains described flame heavenly bamboo cane.
4. according to the tissue culture method of the flame heavenly bamboo based on LED light source described in any one in claims 1 to 3, it is characterized in that, described LED lamp dominant wavelength is 525nm, and wave spectrum scope is 577-492nm, and range of light intensity is 40-70 μ mol/m
2s.
5. the tissue culture and rapid propagation method of the flame heavenly bamboo based on LED light source according to claim 1, it is characterized in that: the concrete steps of step (2) are as follows: adopt plant division propagation and stem section to breed the method combining and carry out shoot proliferation, the cane obtaining after cultivating in step (1) is cut into the little stem section of 0.5cm-1.0cm, each stem section is at least with a blade, then this little stem section is inoculated in and in the blake bottle that adventitious bud proliferation medium is housed, breeds cultivation, the new lateral bud that need to take root is placed in rejuvenation under common fluorescent lamp and cultivates, give illumination every day 16h, need the new lateral bud of subculture to continue to cultivate under LED lamp, propagation multiple reaches 7-8 doubly.
6. the tissue culture and rapid propagation method of the flame heavenly bamboo based on LED light source according to claim 5, it is characterized in that: the formula of described adventitious bud proliferation medium is: WPM medium and MS medium equal-volume mixed liquor, add 2.5mg/L6-BA, 7g/L agar and 30g/L sucrose, pH5.6.
7. the tissue culture and rapid propagation method of the flame heavenly bamboo based on LED light source according to claim 5, it is characterized in that: the concrete steps of step (3) are as follows: the flame heavenly bamboo that process fluorescent lamp in step (2) was carried out to rejuvenation cultivation is inoculated in Rooting and hardening-off culture base, be placed under common fluorescent lamp and carry out culture of rootage, light application time is 12h/d.
8. the tissue culture and rapid propagation method of the flame heavenly bamboo based on LED light source according to claim 7, is characterized in that: Rooting and hardening-off culture based formulas is: WPM+0.5mg/LIBA+7g/L agar+30g/L sucrose, pH5.6.
9. according to the tissue culture and rapid propagation method of the flame heavenly bamboo based on LED light source described in claim 1 or 7, it is characterized in that: the concrete steps of step (4) are as follows: in the time of the long 2-4cm of being of the root obtaining after culture of rootage in step (3), carry out rooting culture, blake bottle was moved in booth after hardening 7-10 days, wash away medium, root soaks the liquor potassic permanganate that mass concentration is 1% and carries out disinfection, and then carries out outdoor transplanting.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104982196A (en) * | 2015-06-26 | 2015-10-21 | 江苏绿苑园林建设有限公司 | Cultivation method for accelerating growth speed of nandina domestica 'Fire power' |
| CN108124683A (en) * | 2017-12-29 | 2018-06-08 | 佛山市三水阳特园艺有限公司 | A kind of signature seeds seedling production method |
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| CN102939843A (en) * | 2012-11-26 | 2013-02-27 | 江苏绿苑园林建设有限公司 | Method for domesticating hardening seedlings of tissue culture seedlings of Nandina domestica firepower |
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| CN103430839A (en) * | 2013-08-01 | 2013-12-11 | 浙江农林大学 | Culture method of ellcalyplus grandis Hill ex Maid tissue culture seedling |
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2014
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Patent Citations (6)
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| CN101044841A (en) * | 2006-12-22 | 2007-10-03 | 上海市农业科学院 | Fast breeding system for flame nandina |
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Cited By (2)
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| CN104982196A (en) * | 2015-06-26 | 2015-10-21 | 江苏绿苑园林建设有限公司 | Cultivation method for accelerating growth speed of nandina domestica 'Fire power' |
| CN108124683A (en) * | 2017-12-29 | 2018-06-08 | 佛山市三水阳特园艺有限公司 | A kind of signature seeds seedling production method |
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