CN103583337A - Solution culture method of cassava seedlings - Google Patents

Abstract

Provided is a solution culture method of cassava seedlings. The solution culture method of the cassava seedlings comprises the steps of using cut germinal caulicle sections as materials instead of seed stems, carrying out rooting and accelerating germination through a nutrient solution culture method, having control over the temperature and illumination to enable the temperature and the illumination to be appropriate, and transplanting the cassava seedlings to land after the cassava seedlings grow new roots and to be in the stage that the number of each seedling ranges from 4 to 6. The solution culture method of the cassava seedlings solves the problem that emergent seedlings are not orderly in the breeding process of the cassava seedlings, solution culture is carried out by using cassava caulicles, and the cassava seedlings which are uniform in growth can be rapidly obtained for land production.

Description

A kind of solution culture method of cassava seedling

Technical field

The invention belongs to a kind of field of plant growing technology, be specifically related to a kind of solution culture method of cassava seedling.

Background technology

Traditional cassava propagation technique be take kind of a stem, terminal bud and axillalry bud and is cultivated as material.Cultural method has soil incubation, matrix to cultivate and organize the methods such as cultivation.

Summary of the invention:

Technical problem to be solved by this invention is irregular problem of emerging in cassava sapling multiplication process, utilizes cassava children stem to carry out water culture, for Production of Large Fields obtains the solution culture method of a kind of cassava seedling of the consistent cassava seedling of growth fast.

The present invention is by achieving the above object by the following technical solutions: a kind of solution culture method of cassava seedling, comprises the steps:

(1) take the segment of cassava children stem is material, removes young tender terminal bud, shears growth 2cm, with the segment of 1 bud point;

(2) the stem section of having sheared is soaked to base portion 15 minutes with the carbendazim of 2000 times of dilutions, then with the root-inducing powder of 1000 times of dilutions, soak base portion 30 minutes;

(3) the young stem segment of processing through step (1)-(2) is first cultivated 1 week with running water, after growing new root, uses nutrient solution instead and cultivate,

Described nutrient solution is 0.3g/LCa (NO ₃) 4H ₂o, 0.12g/LKNO ₃, 0.12g/LMgSO ₄7H ₂o, 0.035g/LKH ₂pO ₄, 0.005g/LEDTA-Fe, 2.86mg/LH ₃bO ₃, 1.81mg/LMnCl ₂4H ₂o, 0.22mg/LZnSO ₄7H ₂o, 0.08mg/LCuSO ₄5H ₂o, 0.09mg/LH ₂moO ₄4H ₂o, nutrient solution pH is 5.5-6.0, nutrient solution every ventilation half an hour once, each 30 minutes, was changed one time of nutrition liquid every 7 days;

(4) culturing room's temperature is 20-25 ℃, and illumination condition is 8000-10000Lux, and incubation time is 10 days, and cassava children stem grew to for 4 leaf phase～6 leaf phases and can transplant to land for growing field crops.

The invention has the advantages that:

1,, with young stem vicariad stem of the same size, avoided planting the stem impact on sprouting germination rate and neat rate not of uniform size;

2, adopt water culture, control nutrition, temperature and illumination condition, accelerate sprouting and sprout speed, improve germination rate and neat rate;

The invention solves irregular problem of emerging in cassava sapling multiplication process, utilize cassava children stem to carry out water culture, for Production of Large Fields obtains the consistent cassava seedling of growth fast.

Embodiment

By the following examples technical scheme of the present invention is further described.

Embodiment 1

The solution culture method of cassava seedling of the present invention, comprises the steps:

(1) take the segment of cassava children stem is material, removes young tender terminal bud, shears growth 2cm, with the segment of 1 bud point;

(2) the stem section of having sheared is soaked to base portion 15 minutes with the carbendazim of 2000 times of dilutions, then with the root-inducing powder of 1000 times of dilutions, soak base portion 30 minutes;

(3) the young stem segment of processing through step (1)-(2) is first cultivated 1 week with running water, after growing new root, use nutrient solution instead and cultivate.

Described nutrient solution is 0.3g/LCa (NO ₃) 4H ₂o, 0.12g/LKNO ₃, 0.12g/LMgSO ₄7H ₂o, 0.035g/LKH ₂pO ₄, 0.005g/LEDTA-Fe, 2.86mg/LH ₃bO ₃, 1.81mg/LMnCl ₂4H ₂o, 0.22mg/LZnSO ₄7H ₂o, 0.08mg/LCuSO ₄5H ₂o, 0.09mg/LH ₂moO ₄4H ₂o, nutrient solution pH is 5.5-6.0, nutrient solution every ventilation half an hour once, each 30 minutes, was changed one time of nutrition liquid every 7 days;

(4) culturing room's temperature is 20-25 ℃, and illumination condition is 8000-10000 lux (Lux), cultivates 10 days, and cassava children stem grew to for 4 leaf phase～6 leaf phases and can transplant to land for growing field crops.

The young stem segment of table 15 kind of Cassava Cultivars is root growth situation and the survival rate after 15 days in water culture.

Cassava Cultivars	Root long average (cm)	Mean elements	Survival rate (%)
				Osmanthus heat 124	25	11	92
Newly select 048	31	19	100
				Spoke selects 01	13	9	100
N1	11	7	83
				N2	11	3	100

Embodiment 2

The impact that tender leaf stem section maturity is sprouted root system

(1) adopt Cassava Cultivars No. N1, wait the young stem newly sending to grow to 30cm long, young stem is cut into totally 10 sections of 3cm long shoot sections, from young stem top to basal part of stem, be numbered 1., 2., 3.,,,,,, 10. number.

(2) all the other steps are with (2)～(4) of embodiment 1.Result is as shown in table 2:

The adventive root of the different stem sections of table 2 is sprouted and survival rate difference

Stem segment number	Root long (cm)	Radical	Survival rate (%)
				①	0	0	0
②	0	0	0
				③	0	0	0
④	0	0	0
				⑤	0	0	0
⑥	0	0	0
				⑦	1	1	10
⑧	3	4	50
				⑨	6	5	96
⑩	13	8	100

Experimental result shows, the adventive root sprouting of young stem segment and the maturity of survival rate and young stem are closely related, selects at least to cut young stem from the position of top 20cm and makes material, is one of key factor of surviving of seedling.

Embodiment 3

The impact that different nutrient formula is sprouted young stem adventive root

Experiment material is No. N1, Cassava Cultivars, and water culture process is with embodiment 1, and wherein culture fluid formula has 3 kinds, is respectively:

?	Nutrient solution one (g/L)	Nutrient solution two (g/L)	Nutrient solution three (g/L)
				Ca(NO 3)·4H 2O	0.3	0.6	1.18
KNO 3	0.12	0.25	0.51
				MgSO 4·7H 2O	0.12	0.24	0.49
KH 2PO 4	0.035	0.07	0.14
				EDTA-Fe	0.005	0.005	0.005
H 3BO 3	2.86×10 -3	2.86×10 -3	2.86×10 -3
				MnCl 2.4H 2O	1.81×10 -3	1.81×10 -3	1.81×10 -3
ZnSO 4.7H 2O	0.22×10 -3	0.22×10 -3	0.22×10 -3
				CuSO 4.5H 2O	0.08×10 -3	0.08×10 -3	0.08×10 -3
H 2MoO 4.4H 2O	0.09×10 -3	0.09×10 -3	0.09×10 -3

Water culture through 10 days, long and the radical of the root of the cassava children stem adventive root after Different Nutrition liquid is cultivated there are no significant difference, compares, and nutrient solution one salinity that adopts is minimum, cost is minimum, so the preferred nutrient solution prescription of the present invention is: 0.3g/LCa (NO ₃) 4H ₂o, 0.12g/LKNO ₃, 0.12g/LMgSO ₄7H ₂o, 0.035g/LKH ₂pO ₄, 0.005g/LEDTA-Fe, 2.86mg/LH ₃bO ₃, 1.81mg/LMnCl ₂4H ₂o, 0.22mg/LZnSO ₄7H ₂o, 0.08mg/LCuSO ₄5H ₂o, 0.09mg/LH ₂moO ₄4H ₂o.

Claims (2)

1. a solution culture method for cassava seedling, is characterized in that, comprises the steps:

(1) take the segment of cassava children stem is material, removes young tender terminal bud, cut into 3cm long, with the segment of 1 bud point;

(2) the stem section of having sheared is soaked to base portion 15 minutes with the carbendazim of 2000 times of dilutions, then with the root-inducing powder of 1000 times of dilutions, soak base portion 30 minutes;

(3) the young stem segment of processing through step (1)-(2) is first cultivated 1 week with running water, after growing new root, uses nutrient solution instead and cultivate,

Described nutrient solution is 0.3～1.18g/LCa (NO ₃) 4H ₂o, 0.12～0.51g/LKNO ₃, 0.12～0.49g/LMgSO ₄7H ₂o, 0.035～0.14g/LKH ₂pO ₄, 0.005g/LEDTA-Fe, 2.86mg/LH ₃bO ₃, 1.81mg/LMnCl ₂4H ₂o, 0.22mg/LZnSO ₄7H ₂o, 0.08mg/LCuSO ₄5H ₂o, 0.09mg/LH ₂moO ₄4H ₂o, nutrient solution pH is 5.5-6.0, nutrient solution every ventilation half an hour once, each 30 minutes, was changed one time of nutrition liquid every 7 days;

(4) culturing room's temperature is 20-25 ℃, and illumination condition is 8000-10000Lux, cultivates 10 days, and cassava children stem grew to for 4 leaf phase～6 leaf phases and can transplant to land for growing field crops.

2. the solution culture method of cassava seedling according to claim 1, is characterized in that, comprises the steps:

(1) take the segment of cassava children stem is material, removes young tender terminal bud, cut into 3cm long, with the segment of 1 bud point;

(2) the stem section of having sheared is soaked to base portion 15 minutes with the carbendazim of 2000 times of dilutions, then with the root-inducing powder of 1000 times of dilutions, soak base portion 30 minutes;

(3) the young stem segment of processing through step (1)-(2) is first cultivated 1 week with running water, after growing new root, uses nutrient solution instead and cultivate,

Described nutrient solution is 0.3g/LCa (NO ₃) 4H ₂o, 0.12g/LKNO ₃, 0.12g/LMgSO ₄7H ₂o, 0.035g/LKH ₂pO ₄, 0.005g/LEDTA-Fe, 2.86mg/LH ₃bO ₃, 1.81mg/LMnCl ₂4H ₂o, 0.22mg/LZnSO ₄7H ₂o, 0.08mg/LCuSO ₄5H ₂o, 0.09mg/LH ₂moO ₄4H ₂o, nutrient solution pH is 5.5-6.0, nutrient solution every ventilation half an hour once, each 30 minutes, was changed one time of nutrition liquid every 7 days;

(4) culturing room's temperature is 20-25 ℃, and illumination condition is 8000-10000Lux, cultivates 10 days, and cassava children stem grew to for 4 leaf phase～6 leaf phases and can transplant to land for growing field crops.