CN106171444A - A kind of cultural method of potato primary stock - Google Patents
A kind of cultural method of potato primary stock Download PDFInfo
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- CN106171444A CN106171444A CN201610573951.7A CN201610573951A CN106171444A CN 106171444 A CN106171444 A CN 106171444A CN 201610573951 A CN201610573951 A CN 201610573951A CN 106171444 A CN106171444 A CN 106171444A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Developmental Biology & Embryology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
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Abstract
The present invention relates to technical field of crop cultivation, the cultural method of a kind of potato primary stock, step includes: detoxic seedling is the most numerous, seedling exercising, transplanting, seedling management, gather in the crops and preserve.The present invention dispenses the link of strong seedling culture before potato tissue culture transplantation of seedlings, and increase inoculum density in the fast numerous link of potato virus-free plantlet, change vaccination ways, it is greatly improved culture medium utilization rate, increase expanding propagation efficiency, reduce electric cost and culture space, save the cost of the fast numerous link of potato tissue culture；Transplant link and reduce density, many strains cuttage, and change weak transplantation of seedlings during traditional transplanting in strong sprout is, in apical wilt after weak transplantation of seedlings survival, broken apical dominance, plant branch increases, individual plant branch increases to 24 from 12, thus improves plant growing way and original silkworm egg yield.The present invention improves fertilization mode and fertilizer type simultaneously, reduces management cost, improves yield.
Description
Technical field
The present invention relates to technical field of crop cultivation, the cultural method of a kind of potato primary stock.
Background technology
Potato primary stock substrate culture at present typically uses tissue cultured seedling strong seedling culture, transplanting in strong sprout, high density before transplanting
The measures such as transplanting, seedling spraying nutritional solution improve yield, and the problem that this technology exists has:
(1) tissue culture seedlings of potatoes strong seedling culture typically requires low-density, control growing way, causes tissue cultured seedling production cost high；
(2) Rhizoma Solani tuber osi is generally individual plant strong sprout and transplants, and because apical dominance is strong, plant branch is few, causes that individual plant knot potato is few, produces
Measure low；
(3) configuration potato nutritional liquid has certain technology requirement, is unfavorable for general operative, and cost is high, time-consuming takes
Work；
(4) potato primary stock high density production tissue cultured seedling demand is big, and numerous condition fast to potato tissue culture proposes higher
Requirement, increase cost, and nutritional solution is mainly inorganic nutrients, and life-time service is easily caused seedbed salinization of soil；
(5) high density causes individual plant fully to grow, and is unfavorable for that earthing operates；Cause individual plant knot potato number little, weight
Amount is light, is unfavorable for follow-up seed production；
(6) high density production causes Later growth, ventilates not smooth, is easily formed hot and humid microenvironment, induces disease.
Summary of the invention
The problem that it is an object of the invention to exist for above-mentioned technology, it is provided that the cultural method of a kind of potato primary stock.
For achieving the above object, the technical solution used in the present invention is: the cultural method of a kind of potato primary stock, step
Including:
(1) detoxic seedling is the most numerous: detoxic seedling uses the method for toothing inoculation to be inoculated into equipped with in the culture bottle of culture medium, and 25
DEG C, cultivate 15-25d under 2000Lx, 16h/8h photoperiod；
(2) seedling exercising: early or mid Febuary tissue cultured seedling transfers to the indoor of scattered light, at normal temperatures seedling exercising 2-3d；
(3) transplant: after seedling exercising terminates, under flowing water, wash the culture medium on tissue cultured seedling off, and remove old root, in sterilization of taking root
Transplant after soaking 25-35min in liquid, water foot water after transplanting and determine root water；
(4) seedling management；
(5) gather in the crops and preserve: mid or late May gathers in the crops, after size fractionation, cold under temperature 2-4 DEG C, humidity 85%-90%
Storehouse is preserved.
Further, in step (1), described fast breeding culture medium is MS+3 × 10-3Paclobutrazol.
Further, in step (1), described detoxic seedling inoculum density is 30-40 strain/bottle.
Further, in step (3), tissue cultured seedling Transplanting Density is seeding row spacing 10cm × 30cm, every nest 3 strain moves.
Further, in step (3), described in disinfectant solution of taking root be 15ppm IBA+10ppm NAA+500 × carbendazim.
Further, in step (3), described cultivation matrix is coconut palm bran, the peat composed of rotten mosses, Vermiculitum and perlite, and its volume ratio is 5:
2:2:1。
Further, in step (4), described seedling management includes:
1. water: one month 1-2 week of early stage/time, January in later stage/time；
2. fill the gaps with seedlings: 3-4 week after transplanting, cut long 4-7cm, have the stem section of the blade of more than 5, immersion 5-in disinfectant solution of taking root
10min, can plant, and waters sufficient water after having planted；
3. ridging: after tissue cultured seedling is transplanted, 4-5 is all, plant height 15-18cm earths up；
4. control Seedling: after ridging, 1-2 is all, plant height 20-25cm, spray 2000 times of 15% paclobutrazol and control plant height；
5. fertilising: transplant 2 weeks pouring 1 ‰ full nutritive water soluble fertilizers+1 ‰ organic bio-fertilizers；Water 2 ‰ after 4 weeks entirely to seek
Support water soluble fertilizer+2 ‰ organic bio-fertilizer, spread fertilizer over the fields conventional compound fertilizer by 20kg/ mu after 6 weeks, and water.
The method have the benefit that:
(1) link of strong seedling culture before the present invention dispenses potato tissue culture transplantation of seedlings, and the most numerous at potato virus-free plantlet
Link increases inoculum density, changes vaccination ways, is greatly improved culture medium utilization rate, increases expanding propagation efficiency, reduces electric cost
And culture space, save the cost of the fast numerous link of potato tissue culture；
(2) present invention transplants link reduction density, many strains cuttage, and changes weak transplantation of seedlings during traditional transplanting in strong sprout is, in
Apical wilt after weak transplantation of seedlings survival, has broken apical dominance, and plant branch increases, and individual plant branch increases to 2-4 from 1-2
Individual, thus improve plant growing way and original silkworm egg yield；
(3) present invention change the pouring of autogamy nutritional solution be organic fungi-manure, full nutrition water-soluble formulations, the collocation of conventional compound fertilizer make
With, greatly reduce fertilizer amount, fertilizer practice is wanted and recruitment, thus cost-effective；
Density changes traditional 200-400 nest/m2, 1 strain/nest be 33.3-66.7/m2Nest, 3 strains/nest.Dramatically saves on group
Seedlings cultivating consumption, from 10-21 bottle/m2Drop to 3-5 bottle/m2；
(4) present invention change seeding row spacing be 5cm × 5-10cm be 10cm × 30cm, considerably increase seeding row spacing, be conducive in
Phase earths up, improves sufficient room for plant strain growth；Ensure that seedbed is ventilated, beneficially disease prevention and control.Plant knot potato number and
Potato block size is greatly improved, and single nest yield brings up to 15-25 from 1-2, and average weight, from 1.5-3g/, brings up to 4-6g/
Individual；While the unit are original silkworm egg number not underproduction, it is greatly enhanced original silkworm egg quality, the most follow-up seed production.
Detailed description of the invention
Below in conjunction with the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described,
Obviously, described embodiment is only a part of embodiment of the present invention rather than whole embodiments.Based in the present invention
Embodiment, the every other embodiment that those of ordinary skill in the art are obtained under not making creative work premise, all
Belong to the scope of protection of the invention.
Embodiment 1
A kind of cultural method of potato primary stock, step includes:
(1) detoxic seedling is the most numerous: detoxic seedling is inoculated into equipped with culture medium (the most numerous by the method for 30 strains/bottle, employing toothing inoculation
Culture medium is MS+3 × 10-3Paclobutrazol) culture bottle in, 25 DEG C, cultivate 15d under 2000Lx, 16h/8h photoperiod；
Wherein, during toothing inoculation i.e. inoculation, mother's Seedling base portion stays a stipes not cut, and continues playback culturing room and cultivates, inoculation
Time, the stem section cut is without being inserted in culture medium, and uniformly pendulum is being cultivated on basal plane.Expanding propagation culture medium requires that hardness is the softest,
It is beneficial to stem section take root and axillary bud sprouting.Without strong seedling culture before transplanting.
(2) seedling exercising: early or mid Febuary tissue cultured seedling transfers to the indoor of scattered light, at normal temperatures seedling exercising 2d；
(3) transplant: after seedling exercising, under flowing water, wash the culture medium on tissue cultured seedling off, and remove old root, at disinfectant solution of taking root
After (disinfectant solution of taking root is 15ppm IBA+10ppm NAA+500 × carbendazim) inner immersion 25min, tissue cultured seedling presses seeding row spacing 10cm
× 30cm, every nest 3 strain transplant to cultivation matrix (cultivation matrix is coconut palm bran, the peat composed of rotten mosses, Vermiculitum and perlite, and its weight ratio is 5:2:
In 2:1), water foot after having transplanted and determine root water；
(4) seedling management；
1. water: one month 1 week/time of early stage, January in later stage/time；
2. fill the gaps with seedlings: after transplanting 3 weeks, cut long 4cm, have the stem section of the blade of more than 5, disinfectant solution of taking root soaks 5min,
Can plant, after having planted, water sufficient water；
3. ridging: tissue cultured seedling transplant after 4 weeks, plant height 15cm, earth up；
4. Seedling is controlled: after ridging 1 week, plant height 20cm, spray 2000 times of 15% paclobutrazol and control plant height；
5. fertilising: transplant 2 weeks pouring 1 ‰ full nutritive water soluble fertilizers+1 ‰ organic bio-fertilizers；Water 2 ‰ after 4 weeks entirely to seek
Support water soluble fertilizer+2 ‰ organic bio-fertilizer, spread fertilizer over the fields conventional compound fertilizer by 20kg/ mu after 6 weeks, and water.
(5) gather in the crops and preserve: mid or late May gathers in the crops, temperature 2 DEG C, 85% time Storage in cold bank of humidity after size fractionation.
Embodiment 2
A kind of cultural method of potato primary stock, step includes:
(1) detoxic seedling is the most numerous: detoxic seedling is inoculated into equipped with culture medium (the most numerous by the method for 35 strains/bottle, employing toothing inoculation
Culture medium is MS+3 × 10-3Paclobutrazol) culture bottle in, 25 DEG C, cultivate 20d under 2000Lx, 16h/8h photoperiod；
Wherein, during toothing inoculation i.e. inoculation, mother's Seedling base portion stays a stipes not cut, and continues playback culturing room and cultivates, inoculation
Time, the stem section cut is without being inserted in culture medium, and uniformly pendulum is being cultivated on basal plane.Expanding propagation culture medium requires that hardness is the softest,
It is beneficial to stem section take root and axillary bud sprouting.Without strong seedling culture before transplanting.
(2) seedling exercising: early or mid Febuary tissue cultured seedling transfers to the indoor of scattered light, at normal temperatures seedling exercising 2.5d；
(3) transplant: after seedling exercising, under flowing water, wash the culture medium on tissue cultured seedling off, and remove old root, at disinfectant solution of taking root
After (disinfectant solution of taking root is 15ppm IBA+10ppm NAA+500 × carbendazim) inner immersion 30min, tissue cultured seedling presses seeding row spacing 10cm
× 30cm, every nest 3 strain transplant to cultivation matrix (cultivation matrix is coconut palm bran, the peat composed of rotten mosses, Vermiculitum and perlite, and its weight ratio is 5:2:
In 2:1), water foot after having transplanted and determine root water；
(4) seedling management；
1. water: one month 1.5 week/time of early stage, January in later stage/time；
2. fill the gaps with seedlings: after transplanting 3.5 weeks, cut long 6cm, have the stem section of the blade of more than 5, disinfectant solution of taking root soaks
7min, can plant, and waters sufficient water after having planted；
3. ridging: tissue cultured seedling transplant after 4.5 weeks, plant height 16cm, earth up；
4. Seedling is controlled: after ridging 1.5 weeks, plant height 23cm, spray 2000 times of 15% paclobutrazol and control plant height；
5. fertilising: transplant 2 weeks pouring 1 ‰ full nutritive water soluble fertilizers+1 ‰ organic bio-fertilizers；Water 2 ‰ after 4 weeks entirely to seek
Support water soluble fertilizer+2 ‰ organic bio-fertilizer, spread fertilizer over the fields conventional compound fertilizer by 20kg/ mu after 6 weeks, and water.
(5) gather in the crops and preserve: mid or late May gathers in the crops, after size fractionation, at temperature 3 DEG C, 87% time Storage in cold bank of humidity.
Embodiment 3
A kind of cultural method of potato primary stock, step includes:
(1) detoxic seedling is the most numerous: detoxic seedling is inoculated into equipped with culture medium (the most numerous by the method for 40 strains/bottle, employing toothing inoculation
Culture medium is MS+3 × 10-3Paclobutrazol) culture bottle in, 25 DEG C, cultivate 25d under 2000Lx, 16h/8h photoperiod；
Wherein, during toothing inoculation i.e. inoculation, mother's Seedling base portion stays a stipes not cut, and continues playback culturing room and cultivates, inoculation
Time, the stem section cut is without being inserted in culture medium, and uniformly pendulum is being cultivated on basal plane.Expanding propagation culture medium requires that hardness is the softest,
It is beneficial to stem section take root and axillary bud sprouting.Without strong seedling culture before transplanting.
(2) seedling exercising: early or mid Febuary tissue cultured seedling transfers to the indoor of scattered light, at normal temperatures seedling exercising 3d；
(3) transplant: after seedling exercising, under flowing water, wash the culture medium on tissue cultured seedling off, and remove old root, at disinfectant solution of taking root
After (disinfectant solution of taking root is 15ppm IBA+10ppm NAA+500 × carbendazim) inner immersion 35min, tissue cultured seedling presses seeding row spacing 10cm
× 30cm, every nest 3 strain transplant to cultivation matrix (cultivation matrix is coconut palm bran, the peat composed of rotten mosses, Vermiculitum and perlite, and its volume ratio is 5:2:
In 2:1), water foot after having transplanted and determine root water；
(4) seedling management；
1. water: one month 2 week/time of early stage, January in later stage/time；
2. fill the gaps with seedlings: after transplanting 4 weeks, cut long 7cm, have the stem section of the blade of more than 5, disinfectant solution of taking root soaks 5-
10min, can plant, and waters sufficient water after having planted；
3. ridging: tissue cultured seedling transplant after 5 weeks, plant height 18cm, earth up；
4. Seedling is controlled: after ridging 2 weeks, plant height 25cm, spray 2000 times of 15% paclobutrazol and control plant height；
5. fertilising: transplant 2 weeks pouring 1 ‰ full nutritive water soluble fertilizers+1 ‰ organic bio-fertilizers；Water 2 ‰ after 4 weeks entirely to seek
Support water soluble fertilizer+2 ‰ organic bio-fertilizer, spread fertilizer over the fields conventional compound fertilizer by 20kg/ mu after 6 weeks, and water.
(5) gather in the crops and preserve: mid or late May gathers in the crops, temperature 4 DEG C, 90% time Storage in cold bank of humidity after size fractionation.
Those skilled in the art of the present technique are appreciated that unless otherwise defined, and all terms used herein (include technology art
Language and scientific terminology) have with the those of ordinary skill in art of the present invention be commonly understood by identical meaning.Also should
Being understood by, those terms defined in such as general dictionary should be understood that the meaning having with the context of prior art
The meaning that justice is consistent, and unless defined as here, will not explain by idealization or the most formal implication.
It should be noted last that: above example is only in order to illustrative not limiting technical scheme, although ginseng
According to above-described embodiment, the present invention is described in detail, it will be apparent to an ordinarily skilled person in the art that: still can be to this
Invention is modified or equivalent, and any modification or partial replacement without departing from the spirit and scope of the present invention, it is equal
Should contain in the middle of scope of the presently claimed invention.
Claims (7)
1. the cultural method of a potato primary stock, it is characterised in that step includes:
(1) detoxic seedling is the most numerous: detoxic seedling uses the method for toothing inoculation to be inoculated into equipped with in the culture bottle of culture medium, 25 DEG C,
15-25d is cultivated under 2000Lx, 16h/8h photoperiod；
(2) seedling exercising: early or mid Febuary tissue cultured seedling transfers to the indoor of scattered light, at normal temperatures seedling exercising 2-3d；
(3) transplant: after seedling exercising terminates, under flowing water, wash the culture medium on tissue cultured seedling off, and remove old root, taking root in disinfectant solution
Transplant after soaking 25-35min, water foot water after transplanting and determine root water；
(4) seedling management；
(5) gather in the crops and preserve: mid or late May gathers in the crops, after size fractionation, freezer storage under temperature 2-4 DEG C, humidity 85%-90%
Hide.
The cultural method of potato primary stock the most according to claim 1, it is characterised in that in step (1), described fast numerous training
Foster base is MS+3 × 10-3Paclobutrazol.
The cultural method of potato primary stock the most according to claim 1, it is characterised in that in step (1), described detoxic seedling
Inoculum density is 30-40 strain/bottle.
The most according to claim 1, the cultural method of potato primary stock, it is characterised in that in step (3), tissue cultured seedling is transplanted
Density is seeding row spacing 10cm × 30cm, every nest 3 strain moves.
The cultural method of potato primary stock the most according to claim 1, it is characterised in that in step (3), described in take root and disappear
Venom is 15ppm IBA+10ppm NAA+500 × carbendazim.
The cultural method of potato primary stock the most according to claim 1, it is characterised in that in step (3), described cultivation base
Matter is coconut palm bran, the peat composed of rotten mosses, Vermiculitum and perlite, and its volume ratio is 5:2:2:1.
The most according to claim 1, the cultural method of potato primary stock, it is characterised in that in step (4), manage described seedling stage
Reason includes:
1. water: one month 1-2 week of early stage/time, January in later stage/time；
2. fill the gaps with seedlings: 3-4 week after transplanting, cut long 4-7cm, have the stem section of the blade of more than 5, immersion 5-in disinfectant solution of taking root
10min, can plant, and waters sufficient water after having planted；
3. ridging: after tissue cultured seedling is transplanted, 4-5 is all, plant height 15-18cm earths up；
4. control Seedling: after ridging, 1-2 is all, plant height 20-25cm, spray 2000 times of 15% paclobutrazol and control plant height；
5. fertilising: transplant 2 weeks pouring 1 ‰ full nutritive water soluble fertilizers+1 ‰ organic bio-fertilizers；2 ‰ full nutritive waters are watered after 4 weeks
Soluble fertilizers+2 ‰ organic bio-fertilizer, spreads fertilizer over the fields conventional compound fertilizer by 20kg/ mu after 6 weeks, and waters.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109042198A (en) * | 2018-10-16 | 2018-12-21 | 云南省农业技术推广总站 | A kind of tissue culture seedlings of potatoes Field planting breeding method |
| CN109906900A (en) * | 2019-04-01 | 2019-06-21 | 云南省农业科学院经济作物研究所 | A kind of potato primary stock mating system |
| CN114982612A (en) * | 2022-05-17 | 2022-09-02 | 新疆农业科学院综合试验场 | Soilless culture substrate and soilless culture method for potatoes |
| CN115024219A (en) * | 2022-06-07 | 2022-09-09 | 江苏农林职业技术学院 | Liquid intermittent immersion cultivation method for potato tissue culture seedlings |
| CN115281158A (en) * | 2022-08-15 | 2022-11-04 | 绵阳市农业科学研究院 | Method for efficient large-scale propagation of phytoseiulus persimilis |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109042198A (en) * | 2018-10-16 | 2018-12-21 | 云南省农业技术推广总站 | A kind of tissue culture seedlings of potatoes Field planting breeding method |
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| CN114982612A (en) * | 2022-05-17 | 2022-09-02 | 新疆农业科学院综合试验场 | Soilless culture substrate and soilless culture method for potatoes |
| CN115024219A (en) * | 2022-06-07 | 2022-09-09 | 江苏农林职业技术学院 | Liquid intermittent immersion cultivation method for potato tissue culture seedlings |
| CN115281158A (en) * | 2022-08-15 | 2022-11-04 | 绵阳市农业科学研究院 | Method for efficient large-scale propagation of phytoseiulus persimilis |
| CN115281158B (en) * | 2022-08-15 | 2023-09-15 | 绵阳市农业科学研究院 | Method for large-scale propagation of phytoseiid chile |
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Application publication date: 20161207 |