CN104770173B - High-efficiency seedling cultivation method for promoting premature tillering of sugarcane tissue cultured seedlings - Google Patents
High-efficiency seedling cultivation method for promoting premature tillering of sugarcane tissue cultured seedlings Download PDFInfo
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- CN104770173B CN104770173B CN201510171633.3A CN201510171633A CN104770173B CN 104770173 B CN104770173 B CN 104770173B CN 201510171633 A CN201510171633 A CN 201510171633A CN 104770173 B CN104770173 B CN 104770173B
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Abstract
The invention discloses a high-efficiency seedling cultivation method for promoting the premature tillering of sugarcane tissue cultured seedlings, and belongs to the technical field of plant tissue culture. The high-efficiency seedling cultivation method for promoting the premature tillering of the sugarcane tissue cultured seedlings comprises the following steps of after the disinfection treatment carried out on a sand bed, hardening the sugarcane tissue cultured seedlings and then cleaning and disinfecting the sugarcane tissue cultured seedlings, afterwards, planting the sugarcane tissue cultured seedlings on the sand bed, watering enough rooting water, covering with a film, thinly applying compound fertilizer to obtain plantlets after sprouts survive, pruning the seedlings and then transplanting the seedlings in a potted tray which uses bagasse nutrient soil as a matrix, after the plantlets survive, pruning and hardening the seedlings, then thinly applying the compound fertilizer, pruning the seedlings after leaves grow too luxuriantly, and then carrying out field planting with soil in a big field, so as to complete the seedling cultivation. By using the high-efficiency seedling cultivation method for promoting the premature tillering of the sugarcane tissue cultured seedlings, the tillering effect of the sugarcane tissue cultured seedlings can be increased furthest; the high-efficiency seedling cultivation method for promoting the premature tillering of the sugarcane tissue cultured seedlings has excellent effects at the multiplication aspect and the strong seedling aspect of the tissue cultured seedlings, is applicable to the large-scale transplantation of the sugarcane tissue cultured seedlings; not only is the quantity of the sprouts of the sugarcane tissue cultured seedlings increased, but also the survival rate is guaranteed; further, the sprouts grow healthily and strongly; the high-efficiency seedling cultivation method for promoting the premature tillering of the sugarcane tissue cultured seedlings has the characteristics of simple operation, high efficiency and further low cost.
Description
Technical field
The present invention relates to a kind of high efficiency seedling cultivating method for promoting sugar-cane tissue culture seedlings early to send out tiller, belongs to plant tissue culture skill
Art field.
Background technology
Survival rate is focused in the generally transplanting of tissue cultured seedling, and have ignored the effect of tiller.Tiller is the plants such as grass family on ground
Below face or it is close to the branch occurred at ground, condition is appropriate, and tiller is just more.The correlational study of sugar-cane tissue culture seedlings tiller at present is also
Have no report, the trophophase of Caulis Sacchari sinensis has an obvious tillering, tillering stage be to increase unit area productive tiller it is crucial when
Phase, with being proportionate property of yield.Existing sugar-cane tissue culture seedlings method for culturing seedlings, growing space are too little or too big, are unfavorable for tissue cultured seedling
Tiller and strong sprout, the control of the condition such as temperature, illumination condition and soil liquid manure is not in place, is to affect sugar-cane tissue culture seedlings tillering capacity
Key factor.
Research shows that the tiller of Caulis Sacchari sinensis is the side shoot formed by low level underground stipes, although the axillary bud on base portion stipes is equal
It is likely to form tiller, but the direct phase such as tillering quantity and the growth conditionss in genotype, population density, soil fertility and seedling stage
Close, environmental factorss and cultivation step can also affect the tiller of Caulis Sacchari sinensis in addition.It is therefore desirable to research affects the factor of Caulis Sacchari sinensis tiller,
Propose the Caulis Sacchari sinensis method for culturing seedlings of high-survival rate and high tillering capacity.
The content of the invention
To solve the problems such as tillering quantity is few, tissue cultured seedling is few, the present invention provides one kind and promotes sugar-cane tissue culture seedlings early to send out tiller
High efficiency seedling cultivating method, improve sugar-cane tissue culture seedlings survive while, improve its effective tillering, increase tissue culture to a certain extent
The quantity of Seedling.
The present invention is realized by following technical proposal：A kind of high efficiency seedling cultivating method for promoting sugar-cane tissue culture seedlings early to send out tiller,
Through following each step：
（1）With river sand as substrate, sand bed is laid, transplanting the last week, with 0.5% carbendazim to sand bed disinfection；
（2）The sugar-cane tissue culture seedlings being placed in bottle, under the conditions of greenhouse, ventilation, natural lighting, seedling exercising 3~4 days, then
Open bottle cap finishing scouring Seedling 2~3 days；
（3）By step（2）After sugar-cane tissue culture seedlings after seedling exercising are cleaned, immersion 10min is carried out with 0.5% carbendazim, then planted
In step（1）Sand bed on, and pour root water, then epiphragma 10~15 days, suitably divulge information mould proof, thin after seedling is survived to apply
Compound fertilizer 1~2 time, then plant 30~40 days and obtain plant；
（4）By step（3）Plant cutting seedlings 1 time, then transplant in using bagasse Nutrition Soil as the hole tray of substrate；
（5）Treat step（4）In plant survive after, cutting seedlings 1 time, and outdoor seedling exercising 20~30 was placed in after 15~20 days
My god, then Bao Shi compound fertilizer 1~2 time treats that leaf growth crosses flower cutting seedlings 2~3 times, to keep certain stand density, promotes
Its tiller occurs, then band soil is colonized in land for growing field crops, that is, complete nursery.
The step（3）Plantation be to be planted by 2~3cm of spacing in the rows, 5~6cm of line-spacing.
The step（3）Epiphragma be, at summer, also to add a cover shading screen.
The step（3）Seedling survive after in sand bed layer overlay bagasse or peat soil.
The step（3）Bao Shi compound fertilizer after, leaf growth is crossed Sheng and needs cutting seedlings 1 time, it is to avoid affect photosynthesis.
The step（4）Bagasse Nutrition Soil be bagasse:Peat soil:Mass ratio=2 of fine clay:1:2 mixing
Thing.
The step（4）The specification of hole tray be a diameter of 7~10cm of cavities, a height of 6~8cm.
The step（4）Transplanting after when there is tiller, be divided into individual plant and planted.
The step（5）Bao Shi compound fertilizer after when there is tiller, be divided into individual plant and planted.
The cutting seedlings is cut off blade total amount 1/2~2/3.
The invention has the advantages that and effect：
When the 1st, transplanted in sand bed first, using more seedlings away from long journey away from implantation methods, so on the one hand ensure that tissue culture
Seedling has certain growing space, divulges information mould proof；On the other hand, the tiller of sugar-cane tissue culture seedlings is conducive to occur.
2nd, by sugar-cane tissue culture seedlings band soil field planting land for growing field crops, it is avoided that and hurts root, improve the survival rate of tissue cultured seedling, so can also make
The secondary tiller for transplanting generation is not separated in field planting, will form the productive tiller of cane seedling, can greatly increase after field planting
The quantity of effect stem, so as to increase its yield.
3rd, the half of blade is only cut off during cutting seedlings to 2/3rds, certain stand density can be kept, promote its tiller to send out
It is raw.Because sugar-cane tissue culture seedlings are when growth reaches certain density, photosynthesis can be affected, and so grow anti-after containing
And influence whether the overall growth promoter of tissue cultured seedling.And growth and development stage actually easily produces more tiller buds, therefore cut off
The half of sugarcane leaf is conducive to producing more tillers to 2/3rds.
4th, the theory of planting season and Effects of Density Caulis Sacchari sinensis tiller during the present invention is utilized, using appropriate transplanting and secondary shifting
Cultivation measure, the rational close planted in sugar-cane tissue culture seedlings growth course can improve tillering capacity, and appropriate thin planting can then improve seedling
It is ventilated, increase photosynthetic rate, reach the purpose of strong sprout, improve survival rate.The present invention can improve Caulis Sacchari sinensis to greatest extent
The tiller efficiency of tissue cultured seedling, has excellent effect in terms of the propagation and strong sprout of tissue cultured seedling, it is adaptable to sugar-cane tissue culture seedlings it is big
Scale is transplanted, and not only increases the quantity of sugar-cane tissue culture seedlings seedling, it is ensured that survival rate, and seedling early growth is healthy and strong, with behaviour
The characteristics of making simple, efficient and with low cost.
Specific embodiment
By following examples, the invention will be further elaborated, but present invention is not limited to the scope.
Embodiment 1
（1）In the April, with river sand as substrate, the wide sand beds of 1.2m are laid using fragment of brick, transplanting the last week, with 0.5%
Carbendazim is to sand bed disinfection；
（2）Using cloud sugarcane 05-51 and cloud sugarcane 06-407 tissue cultured seedlinies as material, the sugar-cane tissue culture seedlings being placed in bottle, with temperature
Room, ventilation, under the conditions of natural lighting, then seedling exercising 4 days opens bottle cap finishing scouring Seedling 2 days；
（3）By step（2）After sugar-cane tissue culture seedlings after seedling exercising are cleaned, immersion 10min is carried out with 0.5% carbendazim, then by strain
Plant in step away from 3cm, line-spacing 5cm（1）Sand bed on, and pour root water, then epiphragma 12 days, suitably divulge information mould proof, treat
Seedling survive after in sand bed layer overlay bagasse or peat soil, Zai Baoshi compound fertilizer 2 times, if leaf growth is crossed Sheng and needs cutting seedlings
1 time, that is, the 1/2~2/3 of blade total amount is cut off, then plant 30 days and obtain plant；
（4）By step（3）Plant cutting seedlings 1 time, that is, cut off the 1/2~2/3 of blade total amount, then transplant in being sought with bagasse
Support soil（Bagasse:Peat soil:Mass ratio=2 of fine clay:1:2 mixture）As in the hole tray of substrate, the specification of hole tray
It is a diameter of 8cm of cavities, a height of 8cm, if having tiller, is divided into individual plant and is planted；
（5）Treat step（4）In plant survive after, cutting seedlings 1 time cuts off the 1/2~2/3 of blade total amount, and at 20 days
After be placed in outdoor seedling exercising 25 days, if then Bao Shi compound fertilizer 1 time have tiller, is divided into individual plant and is planted, treats leaf growth mistake
Flower cutting seedlings 3 times, that is, cut off the 1/2~2/3 of blade total amount, to keep certain stand density, promotes its tiller to occur, then band
Soil is colonized in land for growing field crops, that is, complete nursery.Survival rate is 94.3%, and tillering capacity is 181%, is improve than conventional method nursery tillering capacity
127%。
Embodiment 2
（1）In the August, with river sand as substrate, the wide sand beds of 1.2m are laid using fragment of brick, transplanting the last week, with 0.5%
Carbendazim is to sand bed disinfection；
（2）Using cloud sugarcane 08-1609 tissue cultured seedlinies as material, the sugar-cane tissue culture seedlings being placed in bottle, with greenhouse, ventilation, from
So under illumination condition, then seedling exercising 3 days opens bottle cap finishing scouring Seedling 3 days；
（3）By step（2）After sugar-cane tissue culture seedlings after seedling exercising are cleaned, immersion 10min is carried out with 0.5% carbendazim, then by strain
Plant in step away from 2cm, line-spacing 6cm（1）Sand bed on, and pour root water, then epiphragma 15 days, suitably divulge information mould proof, if
At summer, also add a cover shading screen, after seedling is survived in sand bed layer overlay bagasse or peat soil, Zai Baoshi compound fertilizer
1 time, if leaf growth is crossed Sheng and needs cutting seedlings 1 time, that is, the 1/2~2/3 of blade total amount is cut off, then plant 35 days and obtain plant；
（4）By step（3）Plant cutting seedlings 1 time, that is, cut off the 1/2~2/3 of blade total amount, then transplant in being sought with bagasse
Support soil（Bagasse:Peat soil:Mass ratio=2 of fine clay:1:2 mixture）As in the hole tray of substrate, the specification of hole tray
It is a diameter of 10cm of cavities, a height of 7cm, if having tiller, is divided into individual plant and is planted；
（5）Treat step（4）In plant survive after, cutting seedlings 1 time cuts off the 1/2~2/3 of blade total amount, and at 18 days
After be placed in outdoor seedling exercising 30 days, if then Bao Shi compound fertilizer 2 times have tiller, are divided into individual plant and are planted, treat leaf growth mistake
Flower cutting seedlings 2 times, that is, cut off the 1/2~2/3 of blade total amount, to keep certain stand density, promotes its tiller to occur, then band
Soil is colonized in land for growing field crops, that is, complete nursery.Survival rate is 95.7%, and tillering capacity is 167%, is improve than conventional method nursery tillering capacity
105%。
Embodiment 3
（1）Part in November, with river sand as substrate, lays the wide sand beds of 1.2m using fragment of brick, transplanting the last week, with
0.5% carbendazim is to sand bed disinfection；
（2）Using cloud sugarcane 08-2800 tissue cultured seedlinies as material, the sugar-cane tissue culture seedlings being placed in bottle, with greenhouse, ventilation, from
So under illumination condition, then seedling exercising 4 days opens bottle cap finishing scouring Seedling 3 days；
（3）By step（2）After sugar-cane tissue culture seedlings after seedling exercising are cleaned, immersion 10min is carried out with 0.5% carbendazim, then by strain
Plant in step away from 3cm, line-spacing 6cm（1）Sand bed on, and pour root water, then epiphragma 10 days, suitably divulge information mould proof, if
At summer, also add a cover shading screen, after seedling is survived in sand bed layer overlay bagasse or peat soil, Zai Baoshi compound fertilizer
2 times, if leaf growth is crossed Sheng and needs cutting seedlings 1 time, that is, the 1/2~2/3 of blade total amount is cut off, then plant 40 days and obtain plant；
（4）By step（3）Plant cutting seedlings 1 time, that is, cut off the 1/2~2/3 of blade total amount, then transplant in being sought with bagasse
Support soil（Bagasse:Peat soil:Mass ratio=2 of fine clay:1:2 mixture）As in the hole tray of substrate, the specification of hole tray
It is a diameter of 7cm of cavities, a height of 6cm, if having tiller, is divided into individual plant and is planted；
（5）Treat step（4）In plant survive after, cutting seedlings 1 time cuts off the 1/2~2/3 of blade total amount, and at 15 days
After be placed in outdoor seedling exercising 20 days, if then Bao Shi compound fertilizer 2 times have tiller, are divided into individual plant and are planted, treat leaf growth mistake
Flower cutting seedlings 3 times, that is, cut off the 1/2~2/3 of blade total amount, to keep certain stand density, promotes its tiller to occur, then band
Soil is colonized in land for growing field crops, that is, complete nursery.Survival rate is 96.1%, and tillering capacity is 173%, is improve than conventional method nursery tillering capacity
112%。
Claims (9)
1. a kind of promotion sugar-cane tissue culture seedlings early send out the high efficiency seedling cultivating method of tiller, it is characterised in that through the following steps：
（1）With river sand as substrate, sand bed is laid, transplanting the last week, with 0.5% carbendazim to sand bed disinfection；
（2）The sugar-cane tissue culture seedlings being placed in bottle, under the conditions of greenhouse, ventilation, natural lighting, then seedling exercising 3~4 days is opened
Bottle cap finishing scouring Seedling 2~3 days；
（3）By step（2）After sugar-cane tissue culture seedlings after seedling exercising are cleaned, immersion 10min is carried out with 0.5% carbendazim, then by spacing in the rows 2
~3cm, 5~6cm of line-spacing are planted in step（1）Sand bed on, and pour root water, then epiphragma 10~15 days, it is appropriate to divulge information
It is mould proof, treat that seedling survives Hou Baoshi compound fertilizer 1~2 time, then plant 30~40 days and obtain plant；
（4）By step（3）Plant cutting seedlings 1 time, then transplant in using bagasse Nutrition Soil as the hole tray of substrate；
（5）Treat step（4）In plant survive after, cutting seedlings 1 time, and outdoor seedling exercising was placed in after 15~20 days 20~30 days, so
Hou Baoshi compound fertilizer 1~2 time, treats that leaf growth crosses flower cutting seedlings 2~3 times, then band soil is colonized in land for growing field crops, that is, complete nursery.
2. high efficiency seedling cultivating method according to claim 1, it is characterised in that：Step（3）Described epiphragma be at summer,
Shading screen is added a cover also.
3. high efficiency seedling cultivating method according to claim 1, it is characterised in that：Step（3）Described seedling survive after in sand
Layer overlay bagasse or peat soil on bed.
4. high efficiency seedling cultivating method according to claim 1, it is characterised in that：Step（3）After described Bao Shi compound fertilizer, leaf
Piece grew Sheng needs cutting seedlings 1 time.
5. high efficiency seedling cultivating method according to claim 1, it is characterised in that：Step（4）Described bagasse Nutrition Soil is
Bagasse:Peat soil:Mass ratio=2 of fine clay:1:2 mixture.
6. high efficiency seedling cultivating method according to claim 1, it is characterised in that：Step（4）The specification of described hole tray is cave
7~10cm of bore dia, high 6~8cm.
7. high efficiency seedling cultivating method according to claim 1, it is characterised in that：Step（4）When there is tiller after described transplanting
When, it is divided into individual plant and is planted.
8. high efficiency seedling cultivating method according to claim 1, it is characterised in that：Step（5）After described Bao Shi compound fertilizer when
When having tiller, it is divided into individual plant and is planted.
9. the high efficiency seedling cultivating method according to claim 1 or 4, it is characterised in that：The cutting seedlings cuts off blade total amount
1/2~2/3.
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| CN105557491B (en) * | 2016-01-12 | 2018-06-29 | 广西南亚热带农业科学研究所 | A kind of cane seedlings are planted method |
| CN106332648A (en) * | 2016-08-30 | 2017-01-18 | 广西壮族自治区农业科学院甘蔗研究所 | Single-plant temporary planting method of sugarcane tissue culture rooting bottle seedling |
| CN110710433A (en) * | 2018-07-12 | 2020-01-21 | 东莞市生物技术研究所 | Method for improving field transplanting survival rate of sugarcane tissue culture seedlings |
| CN109197570B (en) * | 2018-10-11 | 2021-09-10 | 云南农业大学 | Method for improving collection amount of sugarcane wild species resource pollen |
| CN114303856A (en) * | 2022-01-05 | 2022-04-12 | 广西壮族自治区农业科学院 | Drought-tolerant sugarcane tissue culture seedling raising method suitable for mechanical planting |
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| CN101361458B (en) * | 2008-09-27 | 2011-09-28 | 云南省农业科学院甘蔗研究所 | Efficient breeding method of sugarcane health seedling |
| CN101707979B (en) * | 2009-11-30 | 2012-07-18 | 广西壮族自治区甘蔗研究所 | Sugarcane tissue-culture-seedling bare-seedling field-transplanting method |
| CN102405837B (en) * | 2011-09-06 | 2013-08-21 | 广西亚热带作物研究所试验站 | Method for transplanting sugarcane tissue culture seedlings to fields from low tunnels |
| CN102379243B (en) * | 2011-09-14 | 2013-03-20 | 云南省农业科学院甘蔗研究所 | Method of tissue culture and rapid propagation of healthy sugarcane seedlings |
| CN102498912B (en) * | 2011-11-23 | 2014-07-23 | 云南省农业科学院甘蔗研究所 | Method for transplanting sugar-cane tissue culture seedlings |
| CN102577785B (en) * | 2011-12-13 | 2013-07-31 | 中国热带农业科学院热带生物技术研究所 | Outdoor transplanting and temporary planting method of stock seedlings of healthy sugarcane seedlings |
| CN103355090B (en) * | 2013-08-01 | 2015-01-21 | 广西南亚热带农业科学研究所 | High-density expanding propagation method of healthy sugarcane seedlings |
| US10767188B2 (en) * | 2013-09-25 | 2020-09-08 | Nutech Ventures | Methods and compositions for obtaining useful plant traits |
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