CN104620840A - Cuttage seedling method for rooting syringa vulgaris L. outside matrices - Google Patents
Cuttage seedling method for rooting syringa vulgaris L. outside matrices Download PDFInfo
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- CN104620840A CN104620840A CN201510101740.9A CN201510101740A CN104620840A CN 104620840 A CN104620840 A CN 104620840A CN 201510101740 A CN201510101740 A CN 201510101740A CN 104620840 A CN104620840 A CN 104620840A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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Abstract
The invention discloses a cuttage seedling method for rooting syringa vulgaris L. outside matrices. The method comprises the following steps: preparation of a cuttage facility, scissoring of cuttings, sterilization, girdling and rooting treatment, cuttage and management after cuttage. The cuttage seedling method has the beneficial effects that healing and root primordia differentiation of the cuttings are effectively promoted, so that the rooting rate, survival rate and maternal plant seed and joint utilization rate of cuttage seedling can be obviously increased; compared with conventional cuttage, the cuttage seedling method has the advantage that the cuttings can root 3-7 days ahead; the probability that the cuttings are infected with diseases and pests can be reduced, thus effectively avoiding rot of the cuttings; the cost is saved; the requirements of the cuttings for the environments of the matrices are reduced, thus reducing the selection and treatment standards of the matrices and expanding the selection range; the cuttage seedling method is suitable for intelligent and standard production and has the advantages of simplicity in operation, labor saving, high economic benefits, and the like.
Description
Technical field
The present invention relates to a kind of cuttage seedling raising method, be specifically related to take root outside a kind of Ou Dingxiang matrix cuttage seedling raising method, belong to technical field of plant propagation.
Background technology
Ou Dingxiang (
syringa vulgaris L.), originate in Europe, Oleaceae Genus Syringa, shrub or dungarunga; Cold-resistant drought-enduring, more barren-resistant, resistant to diseases and insects is strong, and flower amount is large, and pattern enriches, and the fragrance of a flower is unique, is one of seeds that the ornamental value introduced of China is high in recent years; Its economic benefit is higher, and future market demand is huge.The Main Cultivars of current introduction have ‘ He Dayton ' (
syringa vulgaris' Albert F.Holden '), ' wheat is agree ' (
syringa vulgaris' Arch McKean ') ' Mo Sijia ' (
syringa vulgaris' Krasavitsa Moskvy '), ' Qiu Li ' (
syringa vulgaris' Charles Joly '), ' Marianne ' (
syringa vulgaris' Marie Frances '), ' a surname Sha ' (
syringa vulgaris' Sensation '), ' ceramic cobalt blue ' (
syringa vulgaris' Wedgewood Blue ') etc.The modes of reproduction of Ou Dingxiang mainly contains press strip, plant division and cuttage.Press strip and the unsuitable large-scale production of division propagation, be not generally used, the nursery of main dependence cottage propagation.Existing cottage propagation method common ground be cuttings callus and the process of taking root all in matrix, have the following disadvantages:
(1) existing cuttage technique mainly by the cuttings cuttage that processed in different substrates, cuttings callus and the process of taking root are all in matrix, but cutting medium water content is difficult to the constant level maintaining suitable cuttage rooting, water content is too low easily causes cuttings dehydration to be wilted, water content is too high, causes poor air permeability, easily grows various germ and rots.(2) prior art mainly uses the way of bactericidal agent or high temperature treatment to carry out disinfection sterilizing to matrix, to reduce cuttage rotting rate, although can produce certain effect, exists significantly not enough.Use bactericide to there is the shortcomings such as lasting period short, the difficult grasp of dosage, sterilizing be not thorough to the matrix sterilization impact of factor such as to be watered, and dosage is large, cost is high; The easy superinfection germ of high-temperature sterilization and be not suitable for large-scale production.Particularly cuttage in summer, by hot and humid impact, in matrix, various pathogen breeding is fast, and infecting potential is strong, very easily causes cuttings to be rotted, greatly have impact on cuttage survival rate.
Ou Dingxiang cuttage has difficulty of taking root, slow characteristic of taking root, and adopts existing cottage method rooting rate lower, less than 40%.
Summary of the invention
The technical problem to be solved in the present invention is for above deficiency, provides cuttage seedling raising method of taking root outside a kind of Ou Dingxiang matrix, realizes following goal of the invention:
(1) improve cuttage and seedling culture callus induction rate and root restriction differentiation rate, callus induction rate reaches 99.9-100%, and root restriction differentiation rate reaches 99.8-99.9%;
(2) improve the rooting rate of cuttage and seedling culture, rooting rate reaches 99.6-99.8%;
(3) survival rate of cuttage and seedling culture is improved, survival rate 99.8-100%;
(4) improve maternal plant kind joint availability, maternal plant kind joint availability reaches 94-96%;
(5) reduce rootage duration, improve and breed speed;
(6) cost is bred in reduction;
(7) avoid disease worm to infect cuttings, stop rotting of cuttings.
For solving the problems of the technologies described above, by the following technical solutions:
To take root outside a kind of Ou Dingxiang matrix cuttage seedling raising method, comprise the following steps: purchasing of cuttage facility, the clip of cuttings, sterilization, band girdle and rooting treatment, cuttage, management after inserting.
Described cuttage and seedling culture, 6-8 days after cuttage, callus induction rate is 99.9-100%, 40-50 days after cuttage, and root restriction differentiation rate is 99.8-99.9%.
Purchasing of described cuttage facility, cuttage adopts plastic canopy, covers the sunshade net that shading rate is 70% outside canopy film; Cutting medium adopts perlite and the peat composed of rotten mosses, by volume for 1:3 is mixed, is sub-packed in nutritive cube after sterilization, is close to and is thrown in canopy; Carry out disinfection with to canopy inwall and exposed section, ground before cuttage.
The clip of described cuttings, the plant of robust growth is selected grow and enriches, without the full branch of damage by disease and insect, sprout, cuts the section of being cut into, every segment length 12 ~ 15cm, retain 1 ~ 2 blade; Its upper cut truncation, apart from upper bud 0.5 ~ 1cm, lower cut is cut sth. askew.
Described sterilization, band girdle and rooting treatment, use 50% carbendazim to dilute 500 times of liquid to soak the cuttings sheared and Girding cutter and carry out disinfection for 10 minutes, then at distance cuttings base portion 5cm place band girdle, band girdle width 0.5 ~ 1.0cm, divest phloem, retain and form layer, used by band girdle mouth ABT1 root-inducing powder 500ppm solution to soak 10 ~ 15min.
Described cuttage, by saturating for the matrix clear water spray of purchasing, the cutting after process vertically inserts in matrix, and the cuttage degree of depth is to cuttings band girdle place, and band girdle position is exposed to stromal surface.
Described insert after management, comprise period management of taking root, take root after management, growing season management, hardening manage.
Described period management of taking root, the cuttage rooting phase adopts the moisturizing of booth auto-spraying system system temperature control, droplet is less than 5 μm, after cuttage, in canopy, air humidity controls more than 95%, temperature controls at 22-32 DEG C, sprayed the indolebutyric acid solution of dilution 1000 times every 2d by atomizing system to cuttings, connect spray 3 times.
Described take root after management, reduce air humidity and remain on 65-75%, temperature of shed controls below 35 DEG C, and respectively ventilate 0.5h sooner or later.
Described growing season management, each 1 time of foliage-spray potassium dihydrogen phosphate 500 times of liquid, 0.3% ferrous sulfate+urea mixed solution, week about, pre-disease prevention is alternately sprayed with 50% carbendazol wettable powder, 800 times of liquid and 50% thiophanate methyl wetting powder, 800 times of liquid.
Described hardening management, carries out hardening after root system development lignification, extends the time of leaking informaton gradually to leak informaton to whole day, and whole day is leaked informaton after 5-7d and removed canopy film, retains sunshade net, after Routine Management 7-14d, between the lights or the cloudy day transplant, water immediately after transplanting.
Compared with prior art, the invention has the beneficial effects as follows:
(1) by the band girdle process to cuttings, the carbohydrate that upper blade is synthesized is delivered to band girdle place downwards to be assembled, and makes nutrient in the storage of band girdle place, effectively facilitates the callus of cuttings and take root.6-8 days after cuttage, callus induction rate is 99.9-100%, 40-50 days after cuttage, and root restriction differentiation rate is 99.8-99.9%.
(2) significantly improve the rooting rate of cuttage and seedling culture, survival rate and maternal plant kind joint availability, 40-50 days after cuttage, rooting rate is 99.6-99.8%, and rooting rate improves 59.2-61.8%; Survival rate is 99.8-100%, and maternal plant kind joint availability is 94-96%.
(3) cuttage and seedling culture of the present invention, cuttage rooting can shift to an earlier date 3 ~ 7d compared with normal cutting propagation.
(4) band girdle place is as position not contacting substrate of taking root, and disease worm is reduced greatly to the probability that cuttings harm occurs, avoids the rotten impact that water content of substrate is difficult to control and the intrusion of pathogen causes cuttings.
(5) method of the present invention, for taking root outside matrix, reduces the requirement of cuttings to matrix environment, avoids and uses the way of bactericidal agent or high temperature treatment to increase the matrix cost that sterilizing brings that carries out disinfection;
(6) method of the present invention, after cuttings root system penetrates matrix, matrix only need meet the required nutrient of cuttings growth, reduces the requirement of cuttings to matrix environment, reduces selection and the cleanup standard of matrix, expand range of choice.
(7) method of the present invention, suitable intelligent, standardized production, have simple to operate, save the advantages such as labour, economic benefit are high.
Accompanying drawing explanation
Fig. 1 is cuttage schematic diagram.
Embodiment
With concrete case study on implementation, the specific embodiment of the present invention is described below:
To take root outside embodiment 1 one kinds of Ou Dingxiang matrix cuttage seedling raising method, be described for Ou Dingxiang ' Qiu Li ', comprise the following steps:
(1) the purchasing of cuttage facility
Cuttage adopts plastic canopy, the sunshade net that shading rate is 70% is covered outside canopy film, in canopy, auto-spraying system system is installed, droplet is less than 5 μm, cutting medium adopts perlite and the peat composed of rotten mosses, by volume for 1:3 is mixed, be sub-packed in the nutritive cube of 10cm × 15cm after disliking mould Lingshui Spring agent 1500 times of liquid disinfectants with 30%, be close to and be thrown in canopy, both sides are apart from canopy wall 60cm, stay the convenient management of 50 ㎝ passages in the middle of the ㎝ of interval 120, with 50% carbendazol wettable powder, 800 times of liquid, canopy inwall and exposed section, ground are carried out disinfection before inserting.
(2) clip of cuttings
Select spring to grow on the Ou Dingxiang ' Qiu Li ' plant of robust growth enrich, without the full branch of damage by disease and insect, sprout, cut the section of being cut into, every segment length 12cm, epicormic branch cuttage need according to plant leaf blade size reservation 1 ~ 2 blade; Its upper cut truncation, is about 0.5cm apart from upper bud, and lower cut is cut sth. askew and is beneficial to cuttage.
(3) sterilization, band girdle and rooting treatment
50% carbendazol wettable powder, 500 times of immersions are used to steep to enter the cuttings sheared and Girding cutter for 10 minutes
Row sterilization, then at distance cuttings base portion 5cm place band girdle, band girdle width 0.5cm, divests phloem, retains and forms layer, is used by band girdle mouth ABT1 root-inducing powder 500ppm solution to soak 10min.
(4) cuttage
By saturating for the matrix clear water spray of purchasing, the cutting after process vertically inserts in matrix, and the cuttage degree of depth is to cuttings band girdle place, and band girdle position is exposed to stromal surface.
(5) management after inserting
The cuttage rooting phase adopts the moisturizing of booth auto-spraying system system temperature control, droplet is less than 5 μm, for ensureing to take root outside temp. and humidity appropriate substrate in canopy, after cuttage, in canopy, air humidity controls more than 95%, temperature controls at 22 DEG C, spray dilution 1000 times indolebutyric acid solution by atomizing system to cuttings every 2d, connect spray 3 times;
After cuttage root-taking, reduce air humidity and remain on 65%, temperature of shed controls below 35 DEG C, and respectively ventilate 0.5h sooner or later;
Each 1 time of foliage-spray potassium dihydrogen phosphate 500 times of liquid, 0.3% ferrous sulfate+urea mixed solution in vegetative period, week about, alternately sprays pre-disease prevention with 50% carbendazol wettable powder, 800 times of liquid and 50% thiophanate methyl wetting powder, 800 times of liquid;
Carry out hardening after root system development lignification, extending the time of leaking informaton gradually leaks informaton to whole day, and whole day is leaked informaton after 6d and removed canopy film, retains sunshade net, after Routine Management 7d, transplants between the lights, water immediately after transplanting.
Find through observing
Cuttings 6d has callus to produce callus induction rate 100% at band girdle place;
12d part cuttings bears adventive root;
To 24d, most of cuttings root system penetrates in matrix, and part cuttings grows sprouting, and investigation rooting rate reaches 82.4%;
Enter Routine Management after 40d, investigation root restriction differentiation rate is 99.9%, and rooting rate reaches 99.8%, and compared with normal cutting propagation, rooting rate improves 59.8%;
Transplant after Routine Management 10d, Routine Management after transplanting, transplanting survival rate reaches 100%.Maternal plant kind joint availability 96%.
Embodiment 2
To take root outside a kind of Ou Dingxiang matrix cuttage seedling raising method, for a surname Ou Dingxiang ' Sha ' be described, comprise the following steps:
(1) the purchasing of cuttage facility
Cuttage adopts plastic canopy, the sunshade net that shading rate is 70% is covered outside canopy film, in canopy, auto-spraying system system is installed, cutting medium adopts perlite and the peat composed of rotten mosses, by volume for 1:3 is mixed, is sub-packed in the nutritive cube of 10cm × 15cm after disliking mould Lingshui Spring agent 1500 times of liquid disinfectants with 30%, be close to and be thrown in canopy, both sides, apart from canopy wall 55cm, are stayed the convenient management of 50 ㎝ passages, are carried out disinfection before inserting with 50% carbendazol wettable powder, 800 times of liquid to canopy inwall and exposed section, ground in the middle of the ㎝ of interval 120.
(2) clip of cuttings
Summer declares Ou Dingxiang ' Sha of robust growth ' plant is selected grow and enrich, without the branch of damage by disease and insect, cut the section of being cut into, every segment length 14cm, retain 1 ~ 2 blade; Its upper cut truncation, is about 0.7cm apart from upper bud, and lower cut is cut sth. askew and is beneficial to cuttage.
(3) sterilization, band girdle and rooting treatment
50% carbendazol wettable powder, 500 times of immersions are used to steep 10 minutes to the cuttings sheared with carry out disinfection with Girding cutter, then at distance cuttings base portion 5cm place band girdle, band girdle width 0.8cm, divests phloem, retain and form layer, used by band girdle mouth ABT1 root-inducing powder 500ppm solution to soak 12min.
(4) cuttage
By saturating for the matrix clear water spray of purchasing, the cutting after process vertically inserts in matrix, and during cuttage, cutting blade facing one direction, avoids mutually hiding and pasting as far as possible; The cuttage degree of depth is to cuttings band girdle place, and band girdle position is exposed to stromal surface.
(5) management after inserting
The cuttage rooting phase adopts the moisturizing of booth auto-spraying system system temperature control, droplet is less than 5 μm, for ensureing to take root outside temp. and humidity appropriate substrate in canopy, after cuttage, in canopy, air humidity controls more than 95%, temperature controls at 27 DEG C, spray 1000 times indolebutyric acid solution by atomizing system to cuttings every 2d, connect spray 3 times;
Reduce air humidity after cuttage root-taking and remain on 70%, temperature of shed controls below 35 DEG C, and respectively ventilate 0.5h sooner or later;
Each 1 time of foliage-spray potassium dihydrogen phosphate 500 times of liquid, 0.3% ferrous sulfate+urea mixed solution in vegetative period, week about, alternately sprays pre-disease prevention with 50% carbendazol wettable powder, 800 times of liquid and 50% thiophanate methyl wetting powder, 800 times of liquid;
Carry out hardening after root system development lignification, extending the time of leaking informaton gradually leaks informaton to whole day, and whole day is leaked informaton after 5d and removed canopy film, retains sunshade net, after Routine Management 10d, transplants between the lights, water immediately after transplanting.
Find through observing
Cuttings 8d has callus to produce callus induction rate 99.9% at band girdle place;
14d part cuttings bears adventive root;
To 27d, most of cuttings root system penetrates in matrix, and part cuttings grows sprouting, and investigation rooting rate reaches 83.6%;
Enter Routine Management after 45d, investigation root restriction differentiation rate is 99.8%, and rooting rate reaches 99.7%, and compared with normal cutting propagation, rooting rate improves 61.8%;
Transplant after Routine Management 14d, Routine Management after transplanting, transplanting survival rate reaches 99.9%, maternal plant kind joint availability 95%.
Embodiment 3
To take root outside a kind of Ou Dingxiang matrix cuttage seedling raising method, for a surname Ou Dingxiang ' Sha ' be described, comprise the following steps:
(1) the purchasing of cuttage facility
Cuttage adopts plastic canopy, the sunshade net that shading rate is 70% is covered outside canopy film, in canopy, auto-spraying system system is installed, cutting medium adopts perlite and the peat composed of rotten mosses, by volume for 1:3 is mixed, is sub-packed in the nutritive cube of 10cm × 15cm after disliking mould Lingshui Spring agent 1500 times of liquid disinfectants with 30%, be close to and be thrown in canopy, both sides, apart from canopy wall 50cm, are stayed the convenient management of 50 ㎝ passages, are carried out disinfection before inserting with 50% carbendazol wettable powder, 800 times of liquid to canopy inwall and exposed section, ground in the middle of the ㎝ of interval 120.
(2) clip of cuttings
Summer declares Ou Dingxiang ' Sha of robust growth ' plant is selected grow and enrich, without the branch of damage by disease and insect, cut the section of being cut into, every segment length 15cm, retain 1 ~ 2 blade; Its upper cut truncation, is about 1cm apart from upper bud, and lower cut is cut sth. askew and is beneficial to cuttage.
(3) sterilization, band girdle and rooting treatment
50% carbendazol wettable powder, 500 times of immersions are used to steep 10 minutes to the cuttings sheared with carry out disinfection with Girding cutter, then at distance cuttings base portion 5cm place band girdle, band girdle width 1cm, divests phloem, retain and form layer, used by band girdle mouth ABT1 root-inducing powder 500ppm solution to soak 15min.
(4) cuttage
By saturating for the matrix clear water spray of purchasing, the cutting after process vertically inserts in matrix, cutting leaf during cuttage
Sheet facing one direction, is avoided mutually hiding and pasting as far as possible; The cuttage degree of depth is to cuttings band girdle place, and band girdle position is exposed to stromal surface.
(5) management after inserting
The cuttage rooting phase adopts the moisturizing of booth auto-spraying system system temperature control, droplet is less than 5 μm, for ensureing to take root outside temp. and humidity appropriate substrate in canopy, after cuttage, in canopy, air humidity controls more than 95%, temperature controls at 32 DEG C, spray 1000 times indolebutyric acid solution by atomizing system to cuttings every 2d, connect spray 3 times;
Reduce air humidity after cuttage root-taking and remain on 75%, temperature of shed controls below 35 DEG C, and respectively ventilate 0.5h sooner or later;
Each 1 time of foliage-spray potassium dihydrogen phosphate 500 times of liquid, 0.3% ferrous sulfate+urea mixed solution in vegetative period, week about, alternately sprays pre-disease prevention with 50% carbendazol wettable powder, 800 times of liquid and 50% thiophanate methyl wetting powder, 800 times of liquid;
Carry out hardening after root system development lignification, extending the time of leaking informaton gradually leaks informaton to whole day, and whole day is leaked informaton after 7d and removed canopy film, retains sunshade net, after Routine Management 14d, transplants between the lights, water immediately after transplanting.
Find through observing
Cuttings 7d has callus to produce callus induction rate 99.9% at band girdle place;
15d part cuttings bears adventive root;
To 28d, most of cuttings root system penetrates in matrix, and part cuttings grows sprouting, and investigation rooting rate reaches 83.6%;
Enter Routine Management after 50d, investigation root restriction differentiation rate is 99.8%, and rooting rate reaches 99.6%, and compared with normal cutting propagation, rooting rate improves 59.2%;
Transplant after Routine Management 14d, Routine Management after transplanting, transplanting survival rate reaches 99.9%, maternal plant kind joint availability 94%.
Seedling-cultivating method described in embodiment 1-3, cuttage rooting can shift to an earlier date 3 ~ 7d compared with normal cutting propagation, avoids disease worm to infect cuttings, stops rotting of cuttings, has saved seedling cost.
Last it is noted that the foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, although with reference to previous embodiment to invention has been detailed description, for a person skilled in the art, it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein portion of techniques feature.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (8)
1. to take root outside Ou Dingxiang matrix a cuttage seedling raising method, it is characterized in that: comprise the following steps: purchasing of cuttage facility, the clip of cuttings, sterilization, band girdle and rooting treatment, cuttage, management after inserting.
2. take root outside a kind of Ou Dingxiang matrix according to claim 1 cuttage seedling raising method, it is characterized in that: described cuttage and seedling culture, 6-8 days after cuttage, callus induction rate is 99.9-100%, 40-50 days after cuttage, and root restriction differentiation rate is 99.8-99.9%.
3. to take root outside a kind of Ou Dingxiang matrix according to claim 1 cuttage seedling raising method, it is characterized in that: described sterilization, band girdle and rooting treatment, at distance cuttings base portion 5cm place band girdle, band girdle width 0.5 ~ 1.0cm, divest phloem, retain and form layer, used by band girdle mouth ABT1 root-inducing powder 500ppm solution to soak 10 ~ 15min.
4. to take root outside a kind of Ou Dingxiang matrix according to claim 1 cuttage seedling raising method, it is characterized in that: described inserts rear management, comprise period management of taking root: adopt the spraying of auto-spraying system system, droplet is less than 5 μm, after cuttage, in canopy, air humidity controls more than 95%, temperature controls at 22-32 DEG C, is sprayed the indolebutyric acid solution of dilution 1000 times every 2d by atomizing system to cuttings, connects spray 3 times.
5. to take root outside a kind of Ou Dingxiang matrix according to claim 1 cuttage seedling raising method, it is characterized in that: described inserts rear management, comprise growing season management: each 1 time of foliage-spray potassium dihydrogen phosphate 500 times of liquid, 0.3% ferrous sulfate+urea mixed solution, week about, pre-disease prevention is alternately sprayed with 50% carbendazol wettable powder, 800 times of liquid and 50% thiophanate methyl wetting powder, 800 times of liquid.
6. to take root outside a kind of Ou Dingxiang matrix according to claim 1 cuttage seedling raising method, it is characterized in that: described insert after management, comprise after taking root and managing: reduce air humidity and remain on 65-75%, temperature of shed controls below 35 DEG C, respectively ventilate 0.5h sooner or later.
7. to take root outside a kind of Ou Dingxiang matrix according to claim 1 cuttage seedling raising method, it is characterized in that: purchasing of described cuttage facility, cuttage adopts plastic canopy, covers the sunshade net that shading rate is 70% outside canopy film; Cutting medium adopts perlite and the peat composed of rotten mosses, by volume for 1:3 is mixed.
8. to take root outside a kind of Ou Dingxiang matrix according to claim 1 cuttage seedling raising method, it is characterized in that: the clip of described cuttings, every section of long 12 ~ 15cm of cuttings, retains 1 ~ 2 blade; Its upper cut truncation, apart from upper bud 0.5 ~ 1cm, lower cut is cut sth. askew.
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| CN115413491A (en) * | 2022-11-04 | 2022-12-02 | 潍坊市农业科学院(山东省农业科学院潍坊市分院) | Efficient cutting seedling raising method for syringa vulgaris twig |
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