CN103766128A - Asexual rapid propagation method for idesia seedlings - Google Patents
Asexual rapid propagation method for idesia seedlings Download PDFInfo
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- CN103766128A CN103766128A CN201410060289.6A CN201410060289A CN103766128A CN 103766128 A CN103766128 A CN 103766128A CN 201410060289 A CN201410060289 A CN 201410060289A CN 103766128 A CN103766128 A CN 103766128A
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Abstract
The invention discloses an asexual rapid propagation method for idesia seedlings. The asexual rapid propagation method mainly comprises the steps of screening stock plants, cutting orchard and preprocessing, cutting orchard and cutting, raising seedlings and managing, taking the seedling out of gardens and performing subculture. Compared with the tissue culture in the prior art, the asexual rapid propagation method for idesia seedlings is simple in step, low in requirements of technology and devices and low in cost; and meanwhile, the culture of the idesia seedlings adopts branches of the excellent stock plants, so that the large-scale rapid propagation can be realized.
Description
Technical field
The invention belongs to plant Fast Asexual Propagation Technique field, be specifically related to the asexual quick-breeding method of a kind of idesia seedling.
Background technology
Idesia is the partially cloudy seeds of neutrality, happiness light, treelet more resistance to half is shady, the gentle moistening weather of happiness is also more cold-resistant, drought-enduring, soil is required not tight, but in deep, fertile, the moistening sand loam of soil layer well-grown.Be born in the deciduous broadleaved forest such as hillside, mountain depression and mixed coniferous broad leaved forest of low-relief terrain of height above sea level 400-2500 rice, conventionally concentrate and be distributed in the mountain region of 900 meters of height above sea level (Qinling Mountains areas to the south) to height above sea level 1400 meters (southwests).Timber is soft, can be for the material of building, furniture, utensil etc.; For building the fine tree species of fast-growing mixed forest and economic forest in mountain region; How spending fragrance, have nectary, is the nectar source resource plant of apiculture; Tree-like grace, the long order of fruit, result is innumerable, fruit look bright red, likeness in form pearl, wind is curled upwards, and is the ornamental tree species in mountain region, gardens; Fruit, the equal oil-containing of seed; be called the upper oil depot of tree by people; 2011 extensive successfully to try out in aircraft fuel oil, applied value; in " national woods source biomass energy development planning (2011-2020) " that in May, 2013, the State Administration of Forestry promulgated, idesia is classified as to one of more than 30 seeds of shrub of energy large-scale cultivation.
Through pulp oil-containing 43.6% in professional detection idesia, seed oil-containing 22.4～25.9%, average oil content 36.3%, in oil, linoleic acid content is up to 58～81%, and in idesia, the nutritive value of oil is greater than most of high-end woody edible oils as can be seen here.The value of idesia edible oil is also very high.
About the breeding method of idesia seedling, the tissue cultivating method of a kind of idesia is disclosed in Chinese invention patent CN100381045C.It is made up of the following step substantially: 1. by stand-by medium autoclave sterilization; 2. adopting the axillary bud stem section of fine provenance fine individual plant is explant, through alcohol and mercuric chloride solution sterilization, with aseptic water washing; 3. by the explant inoculation medium of sterilization, be placed under the fluorescent lamp manually providing, differentiation grows up to bud seedling; 4. bud seedling is sheared, be inoculated in proliferated culture medium, breed cultivation; 5. by Shoots in vitro, shear, be inoculated in strong seedling culture base, carry out strong seedling culture; 6. test tube is removed to base portion callus and partial blade strong sprout, stay 3～5 blades, be inoculated in root media, carry out culture of rootage; 7. by the seedling with complete, take out and clean, be transplanted in the matrix that contains rice chaff ash and yellow soil, water permeable, shading 70% in first 10 days, after see gradually light, after 20 days, take root and survive, rooting rate reaches more than 85%, after 35～45 days, full exposure.
From the foundation of medium, sterile system, explant, explant propagation, root induction, until required equipment and the technical requirement of each step of hardening is high, production cost is high, is not easy to extensive cultivation.
Summary of the invention
The object of the invention is to: for the problem existing in above-mentioned technical field, provide a kind of idesia seedling asexual quick-breeding method, breeding idesia seedling that can be simple and quick.
To achieve these goals, the technical solution used in the present invention is:
The asexual quick-breeding method of a kind of idesia seedling, comprises the following steps:
The screening of S1, maternal plant: idesia plant is no less than to the Continuous Observation of 3 years, with year the amount of bearing fruit many and fruit is good, oil content is no less than 37%, the plant of implantation time between 8～20 years is as maternal plant;
S2, adopt fringe pre-treatment: cut fringe first 10～30 days, the branch base portion that is 20～90% by degree of lignification on maternal plant, band girdle one encloses the cortex of the wide 3～4mm of being or on branch, twists and hang with iron wire, till making the fracture of idesia branch cortex;
S3, adopt fringe and cuttage: the idesia branch of processing through step 2 is cut; within 4 hours, complete the system of cutting of cuttings; cutting length is got 3～15cm; containing 1～4 internode, 1～3 full bud, lower cut is 1～3mm place under leaf or axillalry bud; upper cut is cut into flat mouth and apart from top bud 0.8～1.2cm; bud in cuttings is all removed, retain 2～4 blades, cuttings base portion is soaked 6～24 hours in the medicament of taking root of low concentration or soak 3～5 seconds in the medicament of taking root of high concentration; First 6 hours of cuttage, carries out disinfection to seedbed; Then by the cuttings cuttage of steeping the medicine of taking root on seedbed;
After S4, seedling management: step S3 completes 1～25 day, seedbed is adopted to micro-spraying, keep medium of seedling bed water content 60～70%, air humidity 80～90%, 15～20 ℃ of ground temperature, 23～27 ℃ of air themperatures; 26～45 days, seedbed is adopted to micro-spraying, keep medium of seedling bed water content 55～60%, air humidity 75～80%, 18～22 ℃ of ground temperature, 24～26 ℃ of air themperatures; 46～60 days, seedbed is adopted to micro-spraying, keep medium of seedling bed water content 60～65%, air humidity 75～80%, 18～22 ℃ of ground temperature, 25～30 ℃ of air themperatures; After 60 days, impose rich water quality management; During whole seedling management, spray 1～3% bordeaux mixture one time at interval of 7～10 days, sprayed 500 times of carbendazols every 10～15 days;
S5, go out garden and subculture and cultivate: treat that idesia height of seedling degree is not less than 30cm, and 20cm place measurement seedling diameter goes out garden while being not less than 0.6cm; Or gather the idesia seedling that is about to out garden, repeating step S2～S4, can obtain the plant that subculture is cultivated.
Described step S2 and S3 adopt root collection and the step of burying to replace, and particularly, choosing and shear diameter from maternal plant root is 0.8～1cm, and the root system of length 8～10cm soaks the root system of having sheared with the medicament of taking root; Concrete immersion process is: in the medicament of taking root of low concentration, soak 1h～6h or soak 1～5 second in the medicament of taking root of high concentration; The medicament of taking root of this low concentration and high concentration adopts the STZ-PP1 type medicament of taking root; Bury first 6 hours, seedbed is carried out disinfection, then the root system of choosing is buried on seedbed.
In described step S3, the take root concentration of medicament of low concentration is 1～10ppm; The take root concentration of medicament of described high concentration is 100～10000ppm.
In described step S2, the diameter of branch base portion is not less than 1cm.
Described step S4's takes root in management process, adopts natural lighting to grow seedlings or full photonursery stock growing, and for cost-saving, first-selected natural lighting is grown seedlings.
Described when seedbed is carried out disinfection, employing be 1～3% formalin thimerosal.
Owing to having adopted technique scheme, the present invention's beneficial effect is compared with prior art:
1, the present invention has avoided laboratory tissue and has cultivated the sport technique segments such as required medium, sterile system, hardening, and step is simplified, method is simple;
2, the present invention can directly realize vegetative propagation large Tanaka, does not need special culture experiment chamber, low to equipment and facility condition requirement, has effectively reduced seedling cost;
3, technical scheme of the present invention can directly be carried out vegetative propagation in large field, the strong adaptability of idesia seedling to soil, and without links such as hardenings, make the survival rate of idesia seedling higher;
4, when technical scheme of the present invention is cultivated idesia seedling, not limited by laboratory, and branch is easy to get, can realizes rapid large-scale and cultivate.
Embodiment
Embodiment 1:
The asexual quick-breeding method of a kind of idesia seedling, comprises the following steps:
S1, maternal plant screening: the idesia plant of wanting to be elected to be maternal plant is no less than to the Continuous Observation of 3 years, and the Yi Genian amount of bearing fruit is many, and fruit is good, substantially there is no biennial bearing phenomenon as candidate maternal plant.Particularly, the candidate maternal plant that selection enters the phase of bearing fruit then, tests its age, after fruit maturation, its fruit is all adopted down and is weighed, and its fruit is sent the same detection method of same testing agency to test its full dried fruit base oil content simultaneously.For the tree at corresponding age, meet the amount of bearing fruit and oil content in table 1, as maternal plant simultaneously.
Table 1
| Age (year) | 8-9 | 10-11 | 12-13 | 14-15 | 16-17 | 18-19 | 20 |
| The amount of bearing fruit (KG) | More than 30 | More than 35 | More than 40 | More than 45 | More than 55 | More than 55 | More than 60 |
| Oil content | More than 37% | More than 37% | More than 37% | More than 37% | More than 37% | More than 37% | More than 37% |
S2, adopt fringe pre-treatment: cut fringe first 10～30 days, the branch base portion that is 20～90% by degree of lignification on maternal plant, band girdle one encloses the cortex of the wide 3～4mm of being or on branch, twists and hang with iron wire, till making the fracture of idesia branch cortex; While doing above-mentioned processing, require branch base diameter to be not less than 1cm.
S3, adopt fringe and cuttage: the idesia branch of doing to adopt fringe pre-treatment is cut, within 4 hours, complete the system of cutting of cuttings, cutting length is got 3～15cm, containing 1～4 internode, 1～3 full bud, lower cut is 1～3mm place under leaf or axillalry bud, upper cut is cut into flat mouth apart from top bud 0.8～1.2cm, bud in cuttings is all removed, retain 2～4 blades, cuttings base portion soaked in the medicament of taking root of low concentration to 6h～24h or soak 3～5 seconds in the medicament of taking root of high concentration.First 6 hours of cuttage, adopt 1～3% formalin thimerosal seedbed is carried out disinfection, then by the cuttings cuttage of steeping the medicine of taking root on seedbed.The take root concentration of medicament of described low concentration is 1～10ppm; The take root concentration of medicament of described high concentration is 100～10000ppm.The medicament of taking root of this low concentration and high concentration adopts the STZ-PP1 type medicament of taking root.
After S4, seedling management: step S3 completes 1～25 day, seedbed is adopted to micro-spraying, keep medium of seedling bed water content 60～70%, air humidity 80～90%, 15～20 ℃ of ground temperature, 23～27 ℃ of air themperatures; 26～45 days, seedbed is adopted to micro-spraying, keep medium of seedling bed water content 55～60%, air humidity 75～80%, 18～22 ℃ of ground temperature, 24～26 ℃ of air themperatures; 46～60 days, seedbed is adopted to micro-spraying, keep medium of seedling bed water content 60～65%, air humidity 75～80%, 18～22 ℃ of ground temperature, 25～30 ℃ of air themperatures; After 60 days, impose rich water quality management; During whole seedling management, spray 1～3% bordeaux mixture one time at interval of 7～10 days, sprayed 500 times of carbendazols every 10～15 days.In the process of whole seedling management, adopt full photonursery stock growing, for cost-saving, first-selected natural light irradiation; Work as bad weather, there is no the solar time, can adopt other illumination to replace, such as incandescent lamp light etc.If employing full photonursery stock growing, will promote idesia seedling rooting 2～7 days in advance.
S5, go out garden and subculture and cultivate: treat that idesia height of seedling degree is not less than 30cm, and 20cm place measurement seedling diameter goes out garden while being not less than 0.6cm; Or gather the idesia seedling that is about to out garden, repeating step S2～S4, can obtain the plant that subculture is cultivated.
Embodiment 2:
The asexual quick-breeding method of a kind of idesia seedling, mainly comprises the following steps:
P1, maternal plant screening: the maternal plant screening technique of the present embodiment is identical with embodiment 1.
P2, Cai Gen and bury: filter out idesia maternal plant according to described maternal plant screening technique, choosing and shear diameter from maternal plant root is 0.8～1cm, the root system of length 8～10cm, is embedded on seedbed after the root system of having sheared was soaked to the medicine of taking root.Immersion process is: in the medicament of taking root of low concentration, soak 1h～6h or soak 1～5 second in the medicament of taking root of high concentration.The take root concentration of medicament of described low concentration is 1～10ppm; The take root concentration of medicament of described high concentration is 100～10000ppm.The medicament of taking root of this low concentration and high concentration adopts the STZ-PP1 type medicament of taking root.Bury first 6 hours, adopt 1～3% formalin thimerosal to carry out disinfection to seedbed, then the root system of choosing is buried on seedbed.
P3, seedling management: bury latter 1～25 day, seedbed is adopted to micro-spraying, keep medium of seedling bed water content 60～70%, air humidity 80～90%, 15～20 ℃ of ground temperature, 23～27 ℃ of air themperatures; Bury latter 26～45 days, seedbed is adopted to micro-spraying, keep medium of seedling bed water content 55～60%, air humidity 75～80%, 18～22 ℃ of ground temperature, 24～26 ℃ of air themperatures; Bury latter 46～60 days, seedbed is adopted to micro-spraying, keep medium of seedling bed water content 60～65%, air humidity 75～80%, 18～22 ℃ of ground temperature, 25～30 ℃ of air themperatures; Bury after latter 60 days, the root of idesia seedling grows up to, and imposes rich water quality management; From root bury seedling grow up to during in, spray 1～3% bordeaux mixture one time at interval of 7～10 days, sprayed 500 times of carbendazols every 10～15 days.In whole process of growing seedlings, adopt natural lighting to grow seedlings.
P4, go out garden and subculture and cultivate: treat that idesia height of seedling degree is not less than 30cm, and 20cm place measurement seedling diameter goes out garden while being not less than 0.6cm; Or gather the idesia seedling that is about to out garden, repeating step P2～P3, can obtain the plant that subculture is cultivated.
Adopt method of the present invention to carry out idesia seedling and breed, step is simple, easily operation.And can directly realize vegetative propagation in land for growing field crops, not need special culture experiment chamber, low to equipment and facility condition requirement, effectively reduce seedling cost, the strong adaptability of idesia seedling to soil, and without links such as hardenings, make the survival rate of seedling high.While using technical scheme of the present invention to cultivate idesia seedling, the cost of idesia seedling is low, and easily breeding adopts successive propagation not to be subject to the impact of the experiment conditions such as group training, is more suitable for rapid large-scale and cultivates.
Claims (6)
1. the asexual quick-breeding method of idesia seedling, is characterized in that, comprises the following steps:
S1, maternal plant screening: idesia plant is no less than to the Continuous Observation of 3 years, good with fruit, oil content is no less than 37%, and the plant of implantation time between 8～20 years is as maternal plant;
S2, adopt fringe pre-treatment: cut fringe first 10～30 days, the branch base portion that is 20-90% by degree of lignification on maternal plant, band girdle one encloses the cortex of the wide 3～4mm of being or on branch, twists and hang with iron wire, till making the fracture of idesia branch cortex;
S3, adopt fringe and cuttage: the idesia branch that will process through step S2 is cut; within 4 hours, complete the system of cutting of cuttings; particularly; cutting length is got 3～15cm; containing 1～4 internode; 1～3 full bud; lower cut is 1～3mm place under leaf or axillalry bud; upper cut is cut into flat mouth and apart from top bud 0.8～1.2cm; bud in cuttings is removed; retain 2～4 blades, cuttings base portion soaked in the medicament of taking root of low concentration to 6h～24h or soak 3～5 seconds in the medicament of taking root of high concentration; First 6 hours of cuttage, carries out disinfection to seedbed; Then by the cuttings cuttage of steeping the medicine of taking root on seedbed;
After S4, seedling management: step S3 completes 1～25 day, seedbed is adopted to micro-spraying, keep medium of seedling bed water content 60～70%, air humidity 80～90%, 15～20 ℃ of ground temperature, 23～27 ℃ of air themperatures; 26～45 days, seedbed is adopted to micro-spraying, keep medium of seedling bed water content 55～60%, air humidity 75～80%, 18～22 ℃ of ground temperature, 24～26 ℃ of air themperatures; 46～60 days, seedbed is adopted to micro-spraying, keep medium of seedling bed water content 60～65%, air humidity 75～80%, 18～22 ℃ of ground temperature, 25～30 ℃ of air themperatures; After 60 days, impose rich water quality management; During whole seedling management, spray 1～3% bordeaux mixture one time at interval of 7～10 days, sprayed 500 times of carbendazols every 10～15 days;
S5, go out garden and subculture and cultivate: treat that idesia height of seedling degree is not less than 30cm, and 20cm place measurement seedling diameter goes out garden while being not less than 0.6cm; Or gather the idesia seedling that is about to out garden, repeating step S2～S4, can obtain the plant that subculture is cultivated.
2. the asexual quick-breeding method of idesia seedling according to claim 1, it is characterized in that, described step S2 and S3 adopt root collection and the step of burying to replace, particularly, choosing and shear diameter from maternal plant root is 0.8～1cm, the root system of length 8～10cm, soaks the root system of having sheared with the medicament of taking root; Concrete immersion process is: root system soaks 1h～6h or soaks 1～5 second in the medicament of taking root of high concentration in the medicament of taking root of low concentration; Bury first 6 hours, seedbed is carried out disinfection, then the root system of choosing is buried on seedbed.
3. the asexual quick-breeding method of idesia seedling according to claim 1 and 2, is characterized in that, the take root concentration of medicament of described low concentration is 1～10ppm; The take root concentration of medicament of described high concentration is 100～10000ppm.
4. the asexual quick-breeding method of idesia seedling according to claim 1, is characterized in that, the diameter of the branch base portion described in step S2 is not less than 1cm.
5. the asexual quick-breeding method of idesia seedling according to claim 1, is characterized in that, in the seedling management process of described step S4, adopts natural lighting to grow seedlings or full photonursery stock growing.
6. the asexual quick-breeding method of idesia seedling according to claim 1 and 2, is characterized in that, described when seedbed is carried out disinfection, employing be 1～3% formalin thimerosal.
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| CN105123282A (en) * | 2015-08-25 | 2015-12-09 | 重庆山林源林业综合开发有限公司 | Idesia grafting method |
| CN105493862A (en) * | 2015-12-24 | 2016-04-20 | 四川万格农业科技有限公司 | Technology for cultivating idesia platform type tree form cutting |
| CN106234222A (en) * | 2016-08-05 | 2016-12-21 | 江苏农林职业技术学院 | A kind of Radix seu Cortex Malloti nepalensis leaf tissue cultural method |
| CN106900509A (en) * | 2017-04-10 | 2017-06-30 | 陕西金油农林科技开发有限公司 | A kind of method of the asexual fast breeding of oily grape nursery stock |
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| CN115039580A (en) * | 2022-03-15 | 2022-09-13 | 中国科学院植物研究所 | Method for cutting propagation by using female plant idesia polycarpa branches in full bearing period |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104620840A (en) * | 2015-03-09 | 2015-05-20 | 山东省潍坊市农业科学院 | Cuttage seedling method for rooting syringa vulgaris L. outside matrices |
| CN105123282A (en) * | 2015-08-25 | 2015-12-09 | 重庆山林源林业综合开发有限公司 | Idesia grafting method |
| CN105493862A (en) * | 2015-12-24 | 2016-04-20 | 四川万格农业科技有限公司 | Technology for cultivating idesia platform type tree form cutting |
| CN106234222A (en) * | 2016-08-05 | 2016-12-21 | 江苏农林职业技术学院 | A kind of Radix seu Cortex Malloti nepalensis leaf tissue cultural method |
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| CN106900509A (en) * | 2017-04-10 | 2017-06-30 | 陕西金油农林科技开发有限公司 | A kind of method of the asexual fast breeding of oily grape nursery stock |
| CN108040728A (en) * | 2017-12-25 | 2018-05-18 | 湖北民族学院 | A kind of idesia female seedling resting bud sprouts branch cuttage breeding method |
| CN108093906A (en) * | 2017-12-28 | 2018-06-01 | 湖北民族学院 | A kind of Cutting root reproduction method of idesia female seedling |
| CN112470729A (en) * | 2020-12-02 | 2021-03-12 | 中国林业科学研究院林业研究所 | Method for raising seedlings of idesia polycarpa stem tip twigs through mist cutting |
| CN115039580A (en) * | 2022-03-15 | 2022-09-13 | 中国科学院植物研究所 | Method for cutting propagation by using female plant idesia polycarpa branches in full bearing period |
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