CN106688556A - High-yield cultivation method of aralia elata seem in barren land - Google Patents

High-yield cultivation method of aralia elata seem in barren land Download PDF

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CN106688556A
CN106688556A CN201611122167.0A CN201611122167A CN106688556A CN 106688556 A CN106688556 A CN 106688556A CN 201611122167 A CN201611122167 A CN 201611122167A CN 106688556 A CN106688556 A CN 106688556A
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seedling
transplanting
days
water
culture
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郭鹏
郝家臣
孙阳
金华
李琳琳
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Dalian Minzu University
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Dalian Nationalities University
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008—Methods for regeneration to complete plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C21/00—Methods of fertilising, sowing or planting
    • A01C21/005—Following a specific plan, e.g. pattern
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00—Cultivation of specific crops or plants not otherwise provided for
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00—Soilless cultivation, e.g. hydroponics
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
    • A01G7/045—Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/001—Culture apparatus for tissue culture
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/14—Measures for saving energy, e.g. in green houses

Abstract

The invention relates to a planting method of wild vegetable, and particularly relates to a high-yield cultivation method of aralia elata seem in a barren land, and belongs to the technical field of rapid propagation of plants. The technical scheme of the invention mainly includes the steps of cleaning and disinfection of an explant, cultivation of multiple shoots, stooling cultivation of multiple shoots, tissue culture seedling domestication, transplantation, and fertilization. The method solves the problems of shortage of sprout source, low survival rate of hardening-seedling and survival rate of transplanting, low output and others when the aralia elata seem is manually planted on a large scale; through the high-yield cultivation method, the sprout expanding propagation efficiency is improved by hundreds of times, the survival rate of hardening-seedling and transplanting is improved to 96% above, the output is improved to 34% above; the method provides powerful guarantee for pushing the industrial and scaled development of aralia elata seem, and has good social and economic benefits.

Description

A kind of method of wasteland high-yield culturing thorn tender bud
Technical field
The present invention relates to wild vegetables cultural method, specially a kind of method of wasteland high-yield culturing thorn tender bud.
Background technology
With the raising of the living standard and the general level of the health of people, people increasingly note nutritive value of food and dietotherapy Value, pollution-free food is favored by people very much, and wild vegetables are exactly one type.Wild vegetables not only have edibility higher, also There is certain value medical health care.In China, edible wild herbs are eaten with a history of thousands of years.Hill vegetable food is nuisanceless, pollution-free, Edible safety.Edible wild vegetables have additional nutrients as vitamin and the supply source of constant or trace element, good for health.
Thorn tender bud is a kind of hill vegetables of vegetal pole horn of plenty, fine containing protein, fat, carbohydrate, meals Dimension, vitamin B2, vitamin B1, niacin, vitamin C, sodium, calcium, magnesium, iron, manganese, zinc, barium, lead, copper, phosphorus, cobalt, chromium, nickel, The compositions such as boron, strontium and peptide, eating method is various, can eat raw, stir-fry and eat, sauce food, cooks soup, cooks filling, or is processed into differently flavoured Small salted vegetables.Delicious fragrant and sweet, the clear tender mellow, game of thorn tender bud is strong, is famous wild vegetables, is described as " kings of wild vegetables ". The area of people's collection throughout the year, due to continuous, a large amount of, extensive predation formula collection for many years and destructive xcessive digging edible wild herbs The presence of resources phenomena, already leads to the exhaustion, Investigation of The Resources of Wild increasingly of the destruction different degrees of to natural vegetation and Investigation of The Resources of Wild Gradually decrease, quality decline, the wild vegetables of collection become more and more thinner, less and less, and some kinds are endangered.Meanwhile, Because the need for the edible wild herbs of natural production can not meet people, people carry out Planting using the method breeding seedling for excavating rhizome Training, has further aggravated the destruction to resource.Artificial breeding, cultivation wild vegetables, have become and meet people's needs and protection open country The important channel of dish resource.
The content of the invention
In order to make up the deficiencies in the prior art, the present invention provides a kind of method of wasteland high-yield culturing thorn tender bud, with expansion The characteristics of numerous speed, hardening and transplanting survival rate high, help to realize standardization, the large-scale development of thorn tender bud as early as possible.This hair Bright technical scheme is as follows：A kind of method of wasteland high-yield culturing thorn tender bud comprises the following steps：
(1) using the stem of thorn tender bud as explant, with sterile water wash it is clean after, be placed in 75vt% alcohol and soak 10 Second, then with aseptic water washing 3-5 time, the mercuric chloride for reusing 0.1vt% soaks 5min, with aseptic water washing 5 times, stem is divided into 2-3 centimetres of stem section；
(2) stem section after step (1) sterilization cleaning is linked into bud inducement cultivation base, in 25 DEG C of illumination cultivations 5 days, is obtained To Multiple Buds；
(3) Multiple Buds induced by step (2) are cut, insertion differential medium under the aseptic, illumination under the conditions of train Support 10-12 days, obtain Multiple Buds；Described differential medium formula：The culture medium based on MS, adds 6- benzamido group purine 3.5-4.0mg/L, methyl α-naphthyl acetate 0.9mg/L, agar 15g/L, sucrose 80g/L, pH are 6.72；
(4) cutting the Multiple Buds that step (3) is obtained carries out culture of putting forth；Put forth prescription of rooting medium：With MS as base Basal culture medium, addition 6- benzamido group purine 5.5mg/L, methyl α-naphthyl acetate 0.3mg/L, agar 12g/L, sucrose 28g/L, pH are 4.64, Cultivate 15-20 days under light illumination, thorn tender bud is long to carry out domestication culture to during more than 5cm as tissue-cultured seedling；
(5) by fertile soil, sandstone and wood sawdust according to 2:1:2 mass ratio is well mixed to make tissue-cultured seedling domestication matrix Standby, domestication stromal thickness is more than 18cm, sand particles size between 0.5-1cm, by tissue culture transplantation of seedlings to taming matrix Upper culture 30 days, domestication matrix is covered using shed, regulation arch temperature of shed at 25-30 DEG C, air humidity in 20%-30%, More than 51% is reached in canopy and scatters light, intensity of illumination 2500-2800lx；It is young as transplanting when tissue-cultured seedling highly reaches more than 15cm Seedling；Seedling will be transplanted, and to dig out holding radicula complete, and the domestication matrix of root is cleaned up with water, is placed under uviol lamp and irradiates 2-3 Minute；Sandy soil by transplanted seedling root using water content between 60-65% are wrapped up；
(6) 1 meter of line-spacing between horizontal ditch, horizontal ditch is opened up wasteland along contour in barren hillside land, depth is 10cm, by step (5) in transplanting seedling replanting to the horizontal ditch after processing, the root for transplanting seedling is stretched naturally and is placed in horizontal ditch, use humic Soil is fixed, and the distance between adjacent transplanting seedling is 50cm；Root water is irrigated in horizontal ditch using the dilution of carbendazim, it Afterwards, a water is sprayed to seedling is transplanted using spray process per 3-5 days；Carbendazim to various crop by fungi (such as Fungi Imperfecti, more son Capsule bacterium) disease that causes has prevention effect, and its active ingredient is N- (2- benzimidazoles base)-methyl carbamate；
(7) 20 days after transplanting, using Water soluble fertilizer, every plant of fertilising 0.15-0.25kg, described Water soluble fertilizer is dilution 800- 1000 times of high nitrogen and potassium composite fertilizer；
(8) after transplanting 3 months, nutrient solution periodically monthly is sprayed until picking time, described nutrition by the way of spray Liquid has following material composition：Magnesium sulfate 120-140mg, ironic citrate 12-14mg, sodium molybdate 0.25mg, asparagine 0.6mg, Glycerine 10mg, deionized water 15mg.
Further, nitrogen content >=16wt% in described high nitrogen and potassium composite fertilizer, potassium content >=21wt%.
Further, the concentration of 6- benzamido group purine is 4.0mg/L in described step (3).
Further, every plant of fertilising 0.25kg in described step (7).
Beneficial effects of the present invention are as follows：
(1) present invention solves the artificial commerial growing of thorn tender bud, and the source of seedling for being faced is in short supply, hardening, transplant survival The problems such as rate is low, yield is not high, serves critical effect, the reproductive speed of seedling is improve hundreds of times, hardening, transplanting Survival rate brings up to more than 96%, and output increased more than 34% is to promote thorn tender bud industrialization, large-scale development as early as possible, there is provided Strong guarantee, with good social and economic benefit；
(2) present invention is during nursery culture, the ratio of appropriate adjustment 6-benzyl aminopurine, methyl α-naphthyl acetate and sucrose and Corresponding pH, filters out optimum prescription of rooting medium of putting forth, it is ensured that culture medium can improve the quality of seedling, grows seedling Stalwartness, root is elongated, flexible, further improves survival rate after transplanting, it is ensured that the planting percent of seedling；
(3) when carrying out nursery using conventional tissue culture mode in the prior art, deserted mountain is directly transplanted after tissue-cultured seedling is tamed In, due to deserted mountain growing environment and tissue culture when growing environment significant difference, easily there is the low problem of transplanting survival rate, this Invention increases ultraviolet irradiation process in the middle of domestication and transplanting process, and it is tender to thorn with culture environment when taming to tame matrix Bud transplanted seedling provides good transitional environment, greatly improves the survival rate of transplanting, and the growing way of thorn tender bud is also obviously improved, So as to improve yield；
(4) growing environment in deserted mountain is severe, monthly fixed the invention provides a kind of nutrient solution for being specially adapted for thorn tender bud Phase sprays, and helps to maintain good growing state, makes grower's income ahead of time.
Specific embodiment
Technical scheme is further described with reference to specific embodiment, but the present invention is not with any shape Formula is limited to embodiment content.Test method described in embodiment unless otherwise specified, is conventional method；Such as without special theory Bright, the reagent and biomaterial are commercially obtained.
Embodiment 1
(1) using the stem of thorn tender bud as explant, with sterile water wash it is clean after, be placed in 75vt% alcohol and soak 10 Second, then with aseptic water washing 3 times, the mercuric chloride immersion 5min of 0.1vt% is reused, with aseptic water washing 5 times, stem is divided into 2 Centimetre stem section；
(2) stem section after step (1) sterilization cleaning is linked into bud inducement cultivation base, in 25 DEG C of illumination cultivations 5 days, is obtained To Multiple Buds；
(3) Multiple Buds induced by step (2) are cut, insertion differential medium under the aseptic, illumination under the conditions of train Support 10 days, obtain Multiple Buds；Described differential medium formula：The culture medium based on MS, adds 6- benzamido group purine 3.5mg/L, methyl α-naphthyl acetate 0.9mg/L, agar 15g/L, sucrose 80g/L, pH are 6.72；
(4) cutting the Multiple Buds that step (3) is obtained carries out culture of putting forth；Put forth prescription of rooting medium：With MS as base Basal culture medium, addition 6- benzamido group purine 5.5mg/L, methyl α-naphthyl acetate 0.3mg/L, agar 12g/L, sucrose 28g/L, pH are 4.64, Cultivate 15 days under light illumination, thorn tender bud is long to carry out domestication culture to during more than 5cm as tissue-cultured seedling；
(5) by fertile soil, sandstone and wood sawdust according to 2:1:2 mass ratio is well mixed to make tissue-cultured seedling domestication matrix Standby, domestication stromal thickness is more than 18cm, sand particles size between 0.5-1cm, by tissue culture transplantation of seedlings to taming matrix Upper culture 30 days, domestication matrix is covered using shed, and at 25 DEG C, air humidity reaches regulation arch temperature of shed 20% in canopy More than 51% scatters light, intensity of illumination 2500lx；As transplanting seedling when tissue-cultured seedling highly reaches more than 15cm；Seedling will be transplanted Dig out holding radicula complete, the domestication matrix of root is cleaned up with water, be placed under uviol lamp and irradiate 2 minutes；By transplanted seedling root Sandy soil of the portion using water content between 60% are wrapped up；
(6) 1 meter of line-spacing between horizontal ditch, horizontal ditch is opened up wasteland along contour in barren hillside land, depth is 10cm, by step (5) in transplanting seedling replanting to the horizontal ditch after processing, the root for transplanting seedling is stretched naturally and is placed in horizontal ditch, use humic Soil is fixed, and the distance between adjacent transplanting seedling is 50cm；Root water is irrigated in horizontal ditch using the dilution of carbendazim, it Afterwards, a water was sprayed to seedling is transplanted using spray process in every 3 days；
(7) 20 days after transplanting, using Water soluble fertilizer, every plant of fertilising 0.15kg, described Water soluble fertilizer is the nitrogen high of 800 times of dilution High potassium compound fertilizer；Nitrogen content 16wt%, potassium content 21wt% in described high nitrogen and potassium composite fertilizer；
(8) after transplanting 3 months, nutrient solution periodically monthly is sprayed until picking time, described nutrition by the way of spray Liquid has following material composition：Magnesium sulfate 120mg, ironic citrate 12mg, sodium molybdate 0.25mg, asparagine 0.6mg, glycerine 10mg, deionized water 15mg.
Embodiment 2
(1) using the stem of thorn tender bud as explant, with sterile water wash it is clean after, be placed in 75vt% alcohol and soak 10 Second, then with aseptic water washing 5 times, the mercuric chloride immersion 5min of 0.1vt% is reused, with aseptic water washing 5 times, stem is divided into 3 Centimetre stem section；
(2) stem section after step (1) sterilization cleaning is linked into bud inducement cultivation base, in 25 DEG C of illumination cultivations 5 days, is obtained To Multiple Buds；
(3) Multiple Buds induced by step (2) are cut, insertion differential medium under the aseptic, illumination under the conditions of train Support 12 days, obtain Multiple Buds；Described differential medium formula：The culture medium based on MS, adds 6- benzamido group purine 4.0mg/L, methyl α-naphthyl acetate 0.9mg/L, agar 15g/L, sucrose 80g/L, pH are 6.72；
(4) cutting the Multiple Buds that step (3) is obtained carries out culture of putting forth；Put forth prescription of rooting medium：With MS as base Basal culture medium, addition 6- benzamido group purine 5.5mg/L, methyl α-naphthyl acetate 0.3mg/L, agar 12g/L, sucrose 28g/L, pH are 4.64, Cultivate 20 days under light illumination, thorn tender bud is long to carry out domestication culture to during more than 5cm as tissue-cultured seedling；
(5) by fertile soil, sandstone and wood sawdust according to 2:1:2 mass ratio is well mixed to make tissue-cultured seedling domestication matrix Standby, domestication stromal thickness is more than 18cm, sand particles size between 0.5-1cm, by tissue culture transplantation of seedlings to taming matrix Upper culture 30 days, domestication matrix is covered using shed, and at 30 DEG C, air humidity reaches regulation arch temperature of shed 30% in canopy More than 51% scatters light, intensity of illumination 2800lx；As transplanting seedling when tissue-cultured seedling highly reaches more than 15cm；Seedling will be transplanted Dig out holding radicula complete, the domestication matrix of root is cleaned up with water, be placed under uviol lamp and irradiate 3 minutes；By transplanted seedling root Sandy soil of the portion using water content between 65% are wrapped up；
(6) 1 meter of line-spacing between horizontal ditch, horizontal ditch is opened up wasteland along contour in barren hillside land, depth is 10cm, by step (5) in transplanting seedling replanting to the horizontal ditch after processing, the root for transplanting seedling is stretched naturally and is placed in horizontal ditch, use humic Soil is fixed, and the distance between adjacent transplanting seedling is 50cm；Root water is irrigated in horizontal ditch using the dilution of carbendazim, it Afterwards, a water was sprayed to seedling is transplanted using spray process in every 5 days；
(7) 20 days after transplanting, using Water soluble fertilizer, every plant of fertilising 0.25kg, described Water soluble fertilizer is the height of 1000 times of dilution Nitrogen high potassium compound fertilizer；
(8) after transplanting 3 months, nutrient solution periodically monthly is sprayed until picking time, described nutrition by the way of spray Liquid has following material composition：Magnesium sulfate 140mg, ironic citrate 14mg, sodium molybdate 0.25mg, asparagine 0.6mg, glycerine 10mg, deionized water 15mg.
Embodiment 3
The present embodiment cultural method is same as Example 2 with the raw material and parameter that use, differs only in, step (5) Ultraviolet irradiation is not carried out after domestication.
Embodiment 4
The present embodiment cultural method is same as Example 2 with the raw material and parameter that use, differs only in, and puts forth and takes root Culture medium prescription, the prescription of rooting medium of putting forth of the present embodiment is the culture medium based on MS, adds 6- benzamido group purine 1mg/L, methyl α-naphthyl acetate 0.1mg/L, agar 12g/L, sucrose 10g/L, pH are 5.80.
Embodiment 5
The present embodiment cultural method is same as Example 2 with the raw material and parameter that use, differs only in, and does not spray battalion Nutrient solution.
Thorn tender bud seedling is cultivated according to the cultural method of embodiment 1-5 respectively, as a result such as table 1 below：
Table 1
Project Transplanting survival rate (%) Yield (kg/m2) Growth cycle (my god)
Embodiment 1 100 15.1 180
Embodiment 2 97 14.6 192
Embodiment 3 41 10.3 134
Embodiment 4 35 11.8 81
Embodiment 5 78 12.5 127
As shown in Table 1, the high yield cultivating method that the present invention is provided greatly improves transplanting survival rate and yield, produces warp Ji benefit is fast.

Claims (4)

1. a kind of method of wasteland high-yield culturing thorn tender bud, it is characterised in that comprise the following steps：
(1) using the stem of thorn tender bud as explant, with sterile water wash it is clean after, be placed in 75vt% alcohol and soak 10 seconds, then With aseptic water washing 3-5 times, the mercuric chloride immersion 5min of 0.1vt% is reused, with aseptic water washing 5 times, stem is divided into 2-3 lis The stem section of rice；
(2) stem section after step (1) sterilization cleaning is linked into bud inducement cultivation base, in 25 DEG C of illumination cultivations 5 days, obtains clump Sprout；
(3) Multiple Buds induced by step (2) are cut, insertion differential medium under the aseptic, illumination under the conditions of cultivate 10- 12 days, obtain Multiple Buds；Described differential medium formula：The culture medium based on MS, addition 6- benzamido group purine 3.5- 4.0mg/L, methyl α-naphthyl acetate 0.9mg/L, agar 15g/L, sucrose 80g/L, pH are 6.72；
(4) cutting the Multiple Buds that step (3) is obtained carries out culture of putting forth；Put forth prescription of rooting medium：Trained based on MS Base is supported, addition 6- benzamido group purine 5.5mg/L, methyl α-naphthyl acetate 0.3mg/L, agar 12g/L, sucrose 28g/L, pH are 4.64, in light According to lower culture 15-20 days, thorn tender bud is long to carry out domestication culture to during more than 5cm as tissue-cultured seedling；
(5) by fertile soil, sandstone and wood sawdust according to 2:1:2 mass ratio is well mixed, and to make tissue-cultured seedling domestication matrix standby With domestication stromal thickness is more than 18cm, and sand particles size is extremely tamed in matrix tissue culture transplantation of seedlings between 0.5-1cm Culture 30 days, domestication matrix is covered using shed, and, at 25-30 DEG C, air humidity is in 20%-30%, canopy for regulation arch temperature of shed Inside reach more than 51% and scatter light, intensity of illumination 2500-2800lx；It is young as transplanting when tissue-cultured seedling highly reaches more than 15cm Seedling；Seedling will be transplanted, and to dig out holding radicula complete, and the domestication matrix of root is cleaned up with water, is placed under uviol lamp and irradiates 2-3 Minute；Sandy soil by transplanted seedling root using water content between 60-65% are wrapped up；
(6) 1 meter of line-spacing between horizontal ditch, horizontal ditch is opened up wasteland along contour in barren hillside land, depth is 10cm, by step (5) place In transplanting seedling replanting to horizontal ditch after reason, the root for transplanting seedling is stretched naturally and is placed in horizontal ditch, it is solid with fertile soil Fixed, the distance between adjacent transplanting seedling is 50cm；Root water is irrigated in horizontal ditch using the dilution of carbendazim, afterwards, A water is sprayed to seedling is transplanted using spray process per 3-5 days；
(7) 20 days after transplanting, using Water soluble fertilizer, every plant of fertilising 0.15-0.25kg, described Water soluble fertilizer is 800-1000 times of dilution High nitrogen and potassium composite fertilizer；
(8) after transplanting 3 months, nutrient solution periodically monthly is sprayed up to picking time by the way of spray, described nutrient solution has Following material composition：Magnesium sulfate 120-140mg, ironic citrate 12-14mg, sodium molybdate 0.25mg, asparagine 0.6mg, glycerine 10mg, deionized water 15mg.
2. a kind of method of wasteland high-yield culturing thorn tender bud as claimed in claim 1, it is characterised in that described high nitrogen and potassium Nitrogen content >=16wt% in composite fertilizer, potassium content >=21wt%.
3. a kind of method of wasteland high-yield culturing thorn tender bud as claimed in claim 1, it is characterised in that described step (3) The concentration of middle 6- benzamido groups purine is 4.0mg/L.
4. a kind of method of wasteland high-yield culturing thorn tender bud as claimed in claim 1, it is characterised in that described step (7) In every plant fertilising 0.25kg.
CN201611122167.0A 2016-12-08 2016-12-08 High-yield cultivation method of aralia elata seem in barren land Pending CN106688556A (en)

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Application publication date: 20170524