CN101473736A - Cuttage and breeding method of Aquilaria sinensis - Google Patents
Cuttage and breeding method of Aquilaria sinensis Download PDFInfo
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- CN101473736A CN101473736A CNA2008101005685A CN200810100568A CN101473736A CN 101473736 A CN101473736 A CN 101473736A CN A2008101005685 A CNA2008101005685 A CN A2008101005685A CN 200810100568 A CN200810100568 A CN 200810100568A CN 101473736 A CN101473736 A CN 101473736A
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Abstract
The invention relates to a novel high-efficiency plant cutting propagation method. The method is characterized by simple operation, year-round production, easy popularization and the like, is highly effective for plants which are usually difficult in survival by cutting propagation, and is especially applicable to a rare plant Aquilaria sinensis. The method can help produce Aquilaria sinensis seedlings in batches, solve serious shortage of Aquilaria sinensis plants in the market, protect and restore endangered wild resources in the natural world, and the method can be used for keeping the quality of the Aquilaria sinensis plants with excellent traits from deterioration. The cutting propagation method usually comprises the following procedures: an annual or perennial branch of the Aquilaria sinensis is taken and treated with a rooting accelerator containing phytohormone, vitamins and the like after proper cutting and processing according to the conventional plant cutting propagation techniques, and the cut branch can root and grow into Aquilaria sinensis seedlings after 40-150 days of proper subsequent supporting management in a cutting medium containing mosses such as sphagnum moss and the like.
Description
Technical field
The present invention relates to the method that a kind of novel plant cuttage is efficiently bred, very effective for the plant that is difficult to the cottage propagation success usually, be a kind of cuttage breeding method that is specially adapted to famous and precious vegetable material whitewood perfume (or spice).
Technical background
Whitewood's perfume (or spice) (Aquilaria sinensis), i.e. buta-buta, the tabernaemontanus bulrush perfume (or spice) that is otherwise known as, LIGNUM AQUILARIAE SINENSIS from Hainan of China, daughter's perfume (or spice), the fragrant tree of tooth etc.Whitewood's perfume is the Thymelaeceae eaglewood, is the distinctive rare protective plant in imminent danger of China, also is unique certified products source that China produces the rare Chinese medicine agalloch eaglewood, still produces the fine tree species of famous and precious natural perfume material and the high-grade ecological garden of construction simultaneously.Because it is limited for whitewood's incense money-gifts to a temple and its monks source electrode, and the market demand is extremely vigorous, places such as Guangdong, Hainan, Yunnan, Fujian have begun to breed in large quantities whitewood's perfume (or spice).
The method of seminal propagation or tissue culture is generally adopted in the breeding of whitewood's perfume (or spice).Method breeding whitewood perfume (or spice) by tissue culture achieve success (Ye Qinfa, Qi Shuyuan and the east of standing in great numbers.Fragrant tissue culture of whitewood and breeding fast.BULLETIN OF BOTANY Vol., 1997,14:60-63; He Xujun etc.The research of agallochum tissue culture rapid propagation technique.Forestry development, 2006,4:10-12), but because the operating technology difficulty is big, sterile production condition harshness, therefore this method is not also produced on probation and is promoted now.The fragrant nursery stock of whitewood in the market all is to utilize the planting seed breeding of whitewood's perfume (or spice) to finish.But, the seed production of annual whitewood's perfume (or spice) is very limited, and seed loses vigor and very rapid, so the planting seed breeding always is limited in about 10 days narrow time limit behind the seed maturity, and the number of growing seedlings simultaneously seriously is subjected to the restriction of seed what and seed vitality.Because the breeding of whitewood's perfume (or spice) is subject to many limitations, various places have been carried out the cuttage test of some whitewood's perfume (or spice) successively, but almost do not have successful story, so think that whitewood's perfume (or spice) is a kind of floristics that is difficult to cuttage.Recently, the cuttage test (Liang Juhong etc. of some whitewood's perfume (or spice) have been carried out in Hainan.The non-tube rapid propagation technical research of the fragrant improved seeds of whitewood.Tropical Forest, 2007,35 (2): 24-25), but cottage propagation is just more effective to the base section of annotinous branch, and cuttage can only be carried out in the winter and spring in Hainan Province.
Summary of the invention
The purpose of this invention is to provide a kind ofly draw materials conveniently, simple to operate, survival rate is high, the anniversary produces, cost is lower, be widely used, the fragrant method of breeding fast of whitewood reliable and stable, easy popularization.This method can be produced the fragrant seedling of whitewood in a large number, thereby solves the serious scarcity and the protection of occurring in nature endangered wild resources of the fragrant seedling of whitewood on the market and recover, and the quality that can be used in the fragrant plant of whitewood that keeps having merit is not degenerated.
This cuttage breeding method, be to utilize whitewood fragrant own regeneration capacity and totipotency, plant cuttage seedling growing process according to routine, get annual or perennial branch of whitewood's perfume (or spice), through suitable block and process after, after handling with the root-inducing promoter that contains vitamin and plant hormone etc., cuttage is in the cutting medium that contains liver moss such as bog moss, through suitable later stage supporting management, just can take root through 40-150 days approximately obtains the fragrant seedling of whitewood.
Concrete scheme of the present invention is: a kind of cuttage breeding method of whitewood's perfume (or spice), carry out according to following step:
(1) processing of Cha Tiaoing
At first select the branch of the fragrant plant of adult whitewood, the spray of Shenging, old branch or perennial branch are all passable then.Branch is blocked cutting into long 5-20 centimetre with sharp blade, and each is transplanted a cutting and leaves 1 bud at least.Cutting has blade or is not with blade all passable, if blade is arranged, removes partial blade, only keeps the 1-2 sheet leaf near the branch upper end, and cuts the 1/2-3/4 of blade, reduces the transpiration of blade to moisture.The lower cut of transplanting a cutting will be not less than 0.5 centimetre from bud, and lower cut is cut sth. askew with sharp blade and is slit into glut shape; Upper cut is near bud, the otch truncation.
(2) preparation of root-inducing promoter
The root-inducing promoter prescription comprises following several:
Indolebutyric acid 0.01-1000mg/L;
Vitamin B1 0.01-1000mg/L;
Silver nitrate 0-1000mg/L;
Distilled water.
(3) preparation of cutting medium
The cutting medium of whitewood's perfume (or spice) is the matrix that contains the bryophyte body.Matrix can be made up of liver moss fully, or liver moss mixes with other cutting medium (as red soil, river sand, vermiculite, wood chip, weeds etc.) commonly used, and wherein the content of liver moss is 20%-100%.The kind of liver moss is a lot, for example liverwort (Hepaticae), angle liverwort (Anthocerotae), bog moss (Sphagnidae), black moss (Andreaeidae) and true moss (Bryidae) etc. only mix all can be used as cutting medium with a kind of liver moss or with several liver mosses.
The cutting medium deactivation sterilization of need sterilizing.The method of sterilization deactivation sterilization is to use water boil 10 minutes to 2 hours, perhaps with sterilizing pan sterilization 10 minutes to 40 minutes, perhaps with disinfectant and cutting medium mixing 1-5 days.After the sterilization, cutting medium is packed in the container or seedbed of plate-like into tiling and compacting, degree of depth 5-30 centimetre.Water irrigates cutting medium subsequently, and its acid-base value maintains pH4.0-7.0.Cutting medium makes the good water-retaining property of cutting medium, soft breathing freely owing to contain a lot of liver mosses, and slant acidity helps antibiotic and the cutting root of hair.
(4) cuttage
The lower cut of transplanting a cutting is immersed in the root-inducing promoter for preparing, and the processing time is no less than 0.5 hour, generally is advisable with 12 hours.Cutting after treatment with flushing with clean water after, be inserted in the cutting medium on the seedbed transplanting a cutting the 1/4-3/4 of bottom, the cuttage distance between every root cutting is no less than 5 centimetres.After cuttage is finished, spray moisture and sterilization ling solution once more, with preserving moisture and cultivate in sealing seedbeds such as plastic film or glass plate.
(5) supporting management after the cuttage
After the cuttage, regularly open seedbed ventilation 10-60 minute of sealing every day and spray moisture.In first month of cuttage, root-inducing promoter of periodically sprinkle and sterilization ling solution weekly; After month of cuttage, urea of periodically sprinkle and sterilization ling solution weekly.The temperature maintenance in seedbed is at 15-30 ℃, and relative moisture maintains 30-100%, transplants a cutting to be under the above environment 40-150 days and can to take root.The cutting of the spray of Shenging, old branch or perennial branch then all can be taken root, and the rooting of cuttings of spray is about 40 days, and the cutting of old branch needs longer rootage duration, but rooting rate is all more than 80%, even near 100%.
Utilize this method to carry out the cottage propagation of whitewood's perfume (or spice) in summer and winter respectively, find season the not significantly restriction of cottage propagation effect, among 1 year whenever all can the cuttage success.
The present invention is bold in innovation on the basis of traditional cuttage breeding method, forms by changing root-inducing promoter and cutting medium, has formed the novel propagation method of plant cuttage efficiently of a cover, has overcome the existing barrier that is difficult to the technology of cutting survival plant.Adopt this method to carry out the cottage propagation of whitewood's perfume (or spice), also have following remarkable result:
(1) do not need seed: the seed resource of whitewood's perfume (or spice) was very rare originally, but its oil content is very high again, has very high commercial value, because this cuttage breeding method does not need seed to breed, so be completely free of dependence, thereby established strong basis for the degree of depth utilization of seed to seed.
(2) no time limit: owing to no longer rely on seed, whenever acquisition and the cuttage success of branch among again can 1 year, thus solved the time restriction of the strictness in the breeding, reduced working strength, for industrialization provides technical support.
(3) production operation is convenient: at first because branch is a large amount of, and also not strict to the development degree requirement of branch, make things convenient for so draw materials abundant; Next is in the entire process process, and supporting management all is easy to realize after cutting, root-inducing promoter, cutting medium and the cuttage, therefore is very easy to operation and popularization.
(4) breeding is stablized fast: only need more a spot of maternal branch material, just can breed the fragrant seedling of a large amount of whitewoods, shortened the breeding time simultaneously, and the reproductive survival rate height, broken away from variation and degeneration that seminal propagation brings, so reliable and stable.
(5) be widely used: owing to changed cutting medium and improved the prescription etc. of root-inducing promoter, make the whitewood's perfume (or spice) that is difficult to the cuttage success be able to success, also have application value very widely for other plant that is difficult to the cuttage success simultaneously.
So, the invention a kind of novel cuttage breeding method efficiently, overcome the shortcoming of present prior art, can unrestrictedly breed the fragrant seedling of whitewood in a large number.Simultaneously, the present invention is to excavation and the breeding of rare appreciable variety agalloch eaglewood, epochmaking meaning is arranged, the effect of understanding the rest by analogy is arranged for some other plant that is difficult to cuttage, for example the agalloch eaglewood that belongs to together with whitewood's perfume (or spice), other contains magnificent cotton rose, greenery cuckoo, carpinus turczaninowii etc.
Concrete enforcement example of the present invention
The concrete example one of implementing
(1) preparation of Cha Tiaoing: get the fragrant branch of whitewood in June, 2007 at random, it is the cutting of 10 centimeter length that branch is blocked, and each cutting top keeps 1 bud at least, makes upper cut from 1 centimetre of bud, and lower cut is away from bud.The upper cut truncation of transplanting a cutting, the lower cut wedgewise of cutting sth. askew keeps and transplants a cutting the 1-2 sheet leaf on top, and cuts 2/3 of blade tip.
(2) root-inducing promoter is handled: the lower cut of transplanting a cutting is immersed in the root-inducing promoter, comprises in the root-inducing promoter that indolebutyric acid is that 50mg/L, vitamin B1 are that 50mg/L, silver nitrate are 0.5mg/L.Cutting submergence 12 hours, submergence is 2 centimetres.
(3) cuttage: cutting is taken out the back and is used flushing with clean water, directly is inserted in the cutting medium.Cutting medium is made up of bog moss fully, and bog moss is tiled in the seedbed that vinyl disc does, and the degree of depth is 15 centimetres after the tiling compacting, is used for cuttage after water irrigates.The degree of depth that the lower cut of transplanting a cutting is inserted into cutting medium is 5 centimetres, and bud is exposed in the air.Transplant a cutting after whole cuttages, spray moisture once more, spray root-inducing promoter and carbendazim solution simultaneously, use the plastic membrane sealing seedbed.
(4) manage after the cuttage: the seedbed that cuttage is good is inserted temperature 20-25 ℃, in the greenhouse or booth of relative moisture 80-90%.Regularly open the plastic film in sealing seedbed every day in first month of cuttage, ventilates and spray moisture and sealing after 20 minutes.In first month of cuttage, root-inducing promoter of periodically sprinkle and sterilization ling solution weekly; After month of cuttage, urea of periodically sprinkle and sterilization ling solution weekly.The cutting of about 40 days left and right sides sprays can be taken root, and the rooting of cuttings of old branch needed more than 100 days, and rooting rate is all more than 80%, even near 100%.After the cottage propagation success, the fragrant seedling of the whitewood of taking root promptly can transplant planting.
The concrete example two of implementing
Specific implementation process in this example is consistent with example one, and just with suitably adjustment such as cutting times, concrete condition is as follows:
(1) cutting time: carry out in November, 2007;
(2) branch of cuttage: get the spray that whitewood's perfume (or spice) is given birth to then;
(3) cutting medium: the mixture of liver moss and red soil, the ratio of the two are 1:1.
About 50 days behind the cottage propagation, the cutting of spray can be taken root, and rooting rate is more than 80%.
Claims (7)
1, the invention belongs to a kind of new and effective plant cuttage propagation method, very effective for the plant that is difficult to the cottage propagation success usually, be a kind of cuttage breeding method that is specially adapted to famous and precious vegetable material whitewood perfume (or spice).This cuttage breeding method, plant cuttage seedling growing process according to routine, get annual or perennial branch of whitewood's perfume (or spice), through suitable block and be processed into cutting after, after handling with the root-inducing promoter that contains plant hormone and vitamin etc. again, cuttage is in the cutting medium that contains liver moss such as bog moss, and through suitable later stage supporting management, just can take root through 40-150 days approximately obtains whitewood's perfume seedling.
2, the plant that is difficult to the cottage propagation success according to claim 1 is characterized in that the floristics that is difficult to the cottage propagation success comprises whitewood's perfume (or spice), and with whitewood's perfume (or spice) relatively near or similar famous and precious rare plants such as agalloch eaglewood, magnificent cotton rose, carpinus turczaninowii.
3, root-inducing promoter according to claim 1 is characterized in that in the root-inducing promoter also containing materials such as vitamin, silver nitrate except that containing plant hormones such as the necessary indolebutyric acid of general hestening rooting.
4, vitamin according to claim 1 is characterized in that vitamin comprises water soluble vitamin and lipovitamin, as Cobastab, vitamin c, vitamin D, vitamin E, vitamin A, vitamin K, biotin etc.
5, the cutting medium that contains liver moss according to claim 1, it is characterized in that containing in the cutting medium liver moss, matrix can be made up of liver moss fully, or liver moss mixes with other cutting medium (as red soil, river sand, vermiculite, wood chip, weeds etc.) commonly used, and wherein the content of liver moss is 20%-100%.
6, liver moss such as bog moss according to claim 1, the kind that it is characterized in that liver mosses such as bog moss is a lot, mainly comprise liverwort (Hepaticae), angle liverwort (Anthocerotae), bog moss (Sphagnidae), black moss (Andreaeidae) and true moss liver mosses such as (Bryidae), only with a kind of liver moss or several liver mosses mixing are all passable.
7, later stage supporting management according to claim 1 is characterized in that the later stage supporting management comprises regularly ventilation and sprays moisture, fertilizer such as disinfectants such as periodically sprinkle root-inducing promoter, sterilization ling, urea.
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| CNA2008101005685A CN101473736A (en) | 2008-05-18 | 2008-05-18 | Cuttage and breeding method of Aquilaria sinensis |
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101731282B (en) * | 2009-12-02 | 2012-03-21 | 中国医学科学院药用植物研究所 | Tambac inducer and preparation method thereof |
| CN104067841A (en) * | 2014-07-15 | 2014-10-01 | 广西壮族自治区林业科学研究院 | Blinding tree twig cutting propagation method |
| CN104170732A (en) * | 2014-08-13 | 2014-12-03 | 安徽医科大学 | Seed tissue culture and propagation method of fourstamen stephania roots |
| CN104855258A (en) * | 2015-04-27 | 2015-08-26 | 南京林业大学 | Hormone treatment method for improving water culture rooting of carpinus betulus |
| CN105009856A (en) * | 2014-04-29 | 2015-11-04 | 朵德祥 | Inula racemosa planting method |
| CN105230321A (en) * | 2015-11-10 | 2016-01-13 | 广东华清园生物科技有限公司 | Cuttage breeding method for borneol type cinnamomum burmannii tree |
| CN105325208A (en) * | 2015-10-30 | 2016-02-17 | 广西八桂凌云茶业有限公司 | Method for promoting rooting of tea tree cutting seedling |
| CN105432293A (en) * | 2015-12-01 | 2016-03-30 | 广西壮族自治区药用植物园 | Tinosporae capillipes cutting propagation method |
| CN105493832A (en) * | 2015-12-09 | 2016-04-20 | 重庆三零三科技有限公司 | Daphone odora Var. Marginata cultivation method |
| CN105940963A (en) * | 2016-05-03 | 2016-09-21 | 蔡楚雄 | Method of low grafting, variety changing and agar forming for agarwood |
| CN109362370A (en) * | 2018-10-31 | 2019-02-22 | 华南农业大学 | A kind of method and its application that toon hardwood cutting is taken root |
| CN110754319A (en) * | 2019-11-26 | 2020-02-07 | 四川云辰园林科技有限公司 | Cultivation method of Christmas kalanchoe |
| CN111990079A (en) * | 2020-10-12 | 2020-11-27 | 湖南衡岳中药饮片有限公司 | Cutting seedling raising method for radix stephaniae tetrandrae |
| CN114402821A (en) * | 2021-12-30 | 2022-04-29 | 海南万安农业开发有限公司 | Method for raising seedlings of semi-lignified twigs of agilawood by cutting |
| CN114831026A (en) * | 2022-05-23 | 2022-08-02 | 中国热带农业科学院热带作物品种资源研究所 | Method for efficiently propagating agilawood tissue culture seedlings by utilizing sterile micro-cutting rooting |
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2008
- 2008-05-18 CN CNA2008101005685A patent/CN101473736A/en active Pending
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| CN101731282B (en) * | 2009-12-02 | 2012-03-21 | 中国医学科学院药用植物研究所 | Tambac inducer and preparation method thereof |
| CN105009856A (en) * | 2014-04-29 | 2015-11-04 | 朵德祥 | Inula racemosa planting method |
| CN104067841B (en) * | 2014-07-15 | 2016-08-24 | 广西壮族自治区林业科学研究院 | A kind of Aquilaria sinensis (Lour.) Spreng. softwood cutting propagation method |
| CN104067841A (en) * | 2014-07-15 | 2014-10-01 | 广西壮族自治区林业科学研究院 | Blinding tree twig cutting propagation method |
| CN104170732A (en) * | 2014-08-13 | 2014-12-03 | 安徽医科大学 | Seed tissue culture and propagation method of fourstamen stephania roots |
| CN104855258A (en) * | 2015-04-27 | 2015-08-26 | 南京林业大学 | Hormone treatment method for improving water culture rooting of carpinus betulus |
| CN105325208A (en) * | 2015-10-30 | 2016-02-17 | 广西八桂凌云茶业有限公司 | Method for promoting rooting of tea tree cutting seedling |
| CN105230321A (en) * | 2015-11-10 | 2016-01-13 | 广东华清园生物科技有限公司 | Cuttage breeding method for borneol type cinnamomum burmannii tree |
| CN105230321B (en) * | 2015-11-10 | 2018-04-13 | 广东华清园生物科技有限公司 | A kind of cuttage breeding method of borneol type burmannii tree |
| CN105432293B (en) * | 2015-12-01 | 2018-08-21 | 广西壮族自治区药用植物园 | Tinosporae cuttage breeding method |
| CN105432293A (en) * | 2015-12-01 | 2016-03-30 | 广西壮族自治区药用植物园 | Tinosporae capillipes cutting propagation method |
| CN105493832A (en) * | 2015-12-09 | 2016-04-20 | 重庆三零三科技有限公司 | Daphone odora Var. Marginata cultivation method |
| CN105940963A (en) * | 2016-05-03 | 2016-09-21 | 蔡楚雄 | Method of low grafting, variety changing and agar forming for agarwood |
| CN109362370A (en) * | 2018-10-31 | 2019-02-22 | 华南农业大学 | A kind of method and its application that toon hardwood cutting is taken root |
| CN110754319A (en) * | 2019-11-26 | 2020-02-07 | 四川云辰园林科技有限公司 | Cultivation method of Christmas kalanchoe |
| CN111990079A (en) * | 2020-10-12 | 2020-11-27 | 湖南衡岳中药饮片有限公司 | Cutting seedling raising method for radix stephaniae tetrandrae |
| CN114402821A (en) * | 2021-12-30 | 2022-04-29 | 海南万安农业开发有限公司 | Method for raising seedlings of semi-lignified twigs of agilawood by cutting |
| CN114831026A (en) * | 2022-05-23 | 2022-08-02 | 中国热带农业科学院热带作物品种资源研究所 | Method for efficiently propagating agilawood tissue culture seedlings by utilizing sterile micro-cutting rooting |
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