CN104350912A - Cutting propagation method for southern blueberries - Google Patents
Cutting propagation method for southern blueberries Download PDFInfo
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- CN104350912A CN104350912A CN201410571420.5A CN201410571420A CN104350912A CN 104350912 A CN104350912 A CN 104350912A CN 201410571420 A CN201410571420 A CN 201410571420A CN 104350912 A CN104350912 A CN 104350912A
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- 238000005520 cutting process Methods 0.000 title claims abstract description 80
- 238000000034 method Methods 0.000 title claims abstract description 31
- 241000609210 Vaccinium tenellum Species 0.000 title claims abstract description 6
- 235000004078 Vaccinium tenellum Nutrition 0.000 title claims abstract description 6
- 235000005609 swamp highbush blueberry Nutrition 0.000 title claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 30
- 238000005507 spraying Methods 0.000 claims abstract description 16
- 206010020649 Hyperkeratosis Diseases 0.000 claims abstract description 4
- 241000993411 Myoporum insulare Species 0.000 claims abstract description 4
- 235000003095 Vaccinium corymbosum Nutrition 0.000 claims description 39
- 235000017537 Vaccinium myrtillus Nutrition 0.000 claims description 39
- 235000021014 blueberries Nutrition 0.000 claims description 39
- 240000000851 Vaccinium corymbosum Species 0.000 claims description 36
- 150000001875 compounds Chemical class 0.000 claims description 34
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 30
- 238000009395 breeding Methods 0.000 claims description 27
- FPAFDBFIGPHWGO-UHFFFAOYSA-N dioxosilane;oxomagnesium;hydrate Chemical compound O.[Mg]=O.[Mg]=O.[Mg]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O FPAFDBFIGPHWGO-UHFFFAOYSA-N 0.000 claims description 25
- 239000007921 spray Substances 0.000 claims description 21
- 239000011159 matrix material Substances 0.000 claims description 19
- YGGXZTQSGNFKPJ-UHFFFAOYSA-N methyl 2-naphthalen-1-ylacetate Chemical compound C1=CC=C2C(CC(=O)OC)=CC=CC2=C1 YGGXZTQSGNFKPJ-UHFFFAOYSA-N 0.000 claims description 19
- 239000000203 mixture Substances 0.000 claims description 18
- JTEDVYBZBROSJT-UHFFFAOYSA-N indole-3-butyric acid Chemical compound C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 claims description 16
- 235000019441 ethanol Nutrition 0.000 claims description 10
- 239000010451 perlite Substances 0.000 claims description 8
- 235000019362 perlite Nutrition 0.000 claims description 8
- 238000004659 sterilization and disinfection Methods 0.000 claims description 7
- 230000006378 damage Effects 0.000 claims description 6
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 4
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 4
- 239000006013 carbendazim Substances 0.000 claims description 4
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- 241000196324 Embryophyta Species 0.000 description 5
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- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical group C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 5
- 241000195940 Bryophyta Species 0.000 description 4
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- 241000208421 Ericaceae Species 0.000 description 1
- 102000018997 Growth Hormone Human genes 0.000 description 1
- 108010051696 Growth Hormone Proteins 0.000 description 1
- 241001529246 Platymiscium Species 0.000 description 1
- 240000008254 Rosa chinensis Species 0.000 description 1
- 235000000664 Rosa chinensis Nutrition 0.000 description 1
- 241000736767 Vaccinium Species 0.000 description 1
- 240000008424 Vaccinium ashei Species 0.000 description 1
- 235000013468 Vaccinium ashei Nutrition 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a cutting propagation method for southern blueberries. The cutting propagation method comprises the following steps: selecting strong branches of blueberry trees, cutting the strong branches into cuttings with a certain length, and putting the cut branches into a water container for standby application; filling a cutting compartment with a cutting medium, pouring water to wet through for leveling compaction, and naturally drying in the shade; in early August to the end of September, selecting the standby cuttings, enabling the base parts of the cuttings to dip rooting powder, then inserting the base parts of the cuttings into the medium, and keeping the cuttings a certain distance; spraying water for the cut cuttings in a fine spraying mode, and adjusting the times of spraying in a day according to the difference of temperature; keeping the greenhouse humidity being 80-90 percent and substrate humidity being 65-75 percent, and lasting for at least 20 days; the base parts of the cuttings start to grow callus, decreasing the times of spraying in a day, and keeping the medium to a principle 'see dry and see wet'. After at least 50 days of cutting, new roots basically grow out, and new branches and leaves grow well. The propagation method has the advantages of simple steps, high rooting percentage and seedling rate, low cost, high easiness in promotion and the like.
Description
Technical field
The invention belongs to blueberry propagation technique field, particularly relate to a kind of cuttage breeding method of blueberry.
Background technology
Blueberry belongs to Ericaceae Vacciniodeae genus vaccinium plant, and have kind more than 400, it is distributed widely in the Northern Hemisphere.China blueberry is produced cultivation and is still in the starting stage, and from Jilin, Shandong, Liaoning etc. economizes and develop into the south of the lower reaches of the Yangtze River gradually.Blueberry is a kind of novel fruits of high nutritive value, and economic worth is high, has good development prospect, but in industrialization is produced, blueberry breeding is a great problem that those skilled in the art face always.Blueberry is generally based on cottage propagation, and blueberry cottage propagation more general small berries fruit tree survival rate is low, and technical requirement is high, in all parts of the country extensive to the research of blueberry cuttage technique.Due to the impact of climatic environmental factor, the cutting plantation difference of same kind in different regions is larger.
Cottage propagation in the past generally adopts following operation:
1, the selection of cutting medium: the main plurality of raw materials that adopts is prepared, and material composition contains river sand, sawdust, the peat composed of rotten mosses, curdy fur moss, perlite etc., and disinfects with 500 ~ 600 times of carbendazim;
2, the selection of root-growing agent: root-growing agent in the past generally uses the methyl α-naphthyl acetate of 2000 ~ 3000ml/l, this root-growing agent process blueberry branch DeGrain, rooting rate is lower.
3, the selection in cuttage period: be generally selected in 3 ~ June cutting experiment better.
The blueberry cultivation root-growing agent adopting methyl α-naphthyl acetate and the composite composition of heteroauxin is referred in CN102138529A Chinese patent literature, it mentions that the root-growing agent formula of use is for methyl α-naphthyl acetate and heteroauxin, and be directly soluble in water, but from the physicochemical property of methyl α-naphthyl acetate, heteroauxin, they are all the solid matters being insoluble in water, being soluble in organic solvent, experiment also shows, methyl α-naphthyl acetate and heteroauxin dissolve insufficient in water, there is deposit seed, be difficult to the effect reaching dispersed and effective utilization.The method adopting indolebutyric acid to make blueberry Rapid Rooting as root-growing agent is referred in CN102613078 Chinese patent literature, what it adopted is dissolve indolebutyric acid with NaOH, use water constant volume, but experiment shows, the rooting efficiency of alone indolebutyric acid is comparatively limited, and still have precipitation with after NaOH dissolving, the constant volume that adds water, do not reach homodisperse effect.In addition, this root-growing agent is mainly applied to plantlet in vitro, is not applied to cuttage branch example.The root-growing agent preparation mentioned in CN102668853A Chinese patent literature take water as lysate, and water dissolves insufficient to indolebutyric acid, placement a few minutes will precipitate, do not reach dispersed effect, also mention in matrix of taking root in the document and have employed the peat composed of rotten mosses, but in peat composed of rotten mosses matrix, germ is many, as sterilization is not thorough during use, to serious sick worm harm be brought to cuttage seeding, affect blueberry seedling quality.
In addition, existing root-growing agent compound method is generally make solvent with water or be first dissolved in alcohol to use water constant volume again, and this kind of method does not consider the abundant dissolving of solute and the sedimentation problem after dissolving, and reduces the effectiveness of root-growing agent; In addition, with alcohol dissolving water constant volume, alcohol is difficult to distribute, and all may injure, reduce further the useful effect of root-growing agent in cuttage to root system of plant and root upper part.
Visible, above-mentioned defect finally all can have influence on the application effect of root-growing agent and the successful reproduction of blueberry.
Summary of the invention
Technical problem to be solved by this invention is, overcomes the deficiency and defect mentioned in above background technology, provides that a kind of step is simple, rooting rate and planting percent is higher, cost is low, the cuttage breeding method of the southern blueberry of easy popularization.
For solving the problems of the technologies described above, the technical scheme that the present invention proposes is a kind of cuttage breeding method of southern blueberry, comprises the following steps:
A prepared by () cutting: select annual, semi-lignified in more than 3 years age blueberry tree, healthy and strong branch without damage by disease and insect, cut and put into the bucket filling water and make it not dewater, in the cool branch segmentation is cut into the cutting of certain length, water container is put in cutting for subsequent use;
B () matrix prepares: inserted by cutting medium in cuttage railway carriage or compartment, and trickle drenched leveling compacting, dries in the shade naturally, the front spraying disinfection of cuttage;
C () cuttage: in first arrival in August by the end of September, chooses above-mentioned cutting for subsequent use, cutting base portion is stained with pulvis of taking root, insert in matrix, each cutting keeps a determining deviation;
(d) water spray management: water spray management is carried out to the cutting after above-mentioned cuttage, adopt thin spray pattern to spray water and (must guard against pouring, spraying can adopt agroatomizer or automatic spraying system), according to temperature difference adjustment day spray timings, and keep canopy humidity 80% ~ 90%, soil humidity is 65% ~ 75%, continue at least 20 days, when cutting base portion starts to bear callus, gradually reduce a day spray timings, keep matrix see dry see wet, until cuttage after at least 50 days cutting substantially grow new root, and new branches and leaves grow fine.
Above-mentioned cuttage breeding method, preferably, in described step (a), the cutting cut to lengthen be cut at 10cm ~ 12cm, and each cutting keeps more than 2 leaf buds.
Above-mentioned cuttage breeding method, preferably, in described step (b), the matrix of described cuttage selects pure perlite, and spraying disinfection selects 500 times of liquid carbendazim.The cutting medium that the present invention preferably uses is pure perlite (namely not containing other compositions), it has good permeability, sterilization is convenient, thorough, avoid and add the problem that other materials easily carries germ, can guarantee that cuttage seeding is without courses of infection, sapling is healthy and strong.
Above-mentioned cuttage breeding method, preferably, in described step (c), insert the degree of depth at least 3cm of matrix, the spacing of each cutting remains on 6cm ~ 10cm.
Above-mentioned cuttage breeding method, preferably, in described step (d), refers to according to temperature difference adjustment day spray timings: at least sprayed 1 time every 2 hours without during rain megathermal climate; Temperature, 25 DEG C ~ 30 DEG C time, was at least sprayed 1 time every 4 hours; Each spraying duration controls at 10s ~ 30s.
Above-mentioned cuttage breeding method, preferably, described in pulvis of taking root be compound pulvis of taking root, described compound pulvis of taking root is mixed by pulvis A and pulvis B compound;
Described pulvis A is formulated primarily of following mass fraction component:
Methyl α-naphthyl acetate: 1 ~ 2 part;
Talcum powder: 1000 parts;
Described pulvis B is formulated primarily of following mass fraction component:
Indolebutyric acid: 0.5 ~ 1 part;
Talcum powder: 1000 parts.
Above-mentioned cuttage breeding method, preferably, mass ratio when described pulvis A and pulvis B mixes is 2 ~ 1: 1.
Above-mentioned cuttage breeding method, preferably, described talcum powder is 1200 object industrial talc powder.Talcum powder of the present invention belongs to auxiliary agent, adopts talcum powder not only to draw materials easily as auxiliary agent, and material non-toxic, tasteless, and not having any physics and chemistry to cuttage branch affects, and uses also very convenient.In addition, the present invention selects 1200 object industrial talc powder as the auxiliary agent of methyl α-naphthyl acetate and indolebutyric acid, is because the talcum powder of this granularity is enough fine and smooth on the one hand, grinds that to mix the uniformity fine, can not affect the effect of pulvis of taking root, the talcum powder of this granularity also more economically on the other hand.
Above-mentionedly preferably compoundly take root pulvis mainly based on following principle: first clearly selected two kinds of principal component materials, one is methyl α-naphthyl acetate (1-Naphthaleneacetic acid), and being called for short NAA, is a kind of organic compound (C
10h
7cH
2cO
2h), belong to auxins plant modifying agent, except there is the basic function of general growth hormone, the formation of plant adventive root and root can also be promoted.But our research shows, alone methyl α-naphthyl acetate, though crop rooting promoting is good, have the side effect suppressing terrestrial stem, branch growth under higher concentration, seedling growth is undesirable.Another kind of principal component is indolebutyric acid, and molecular formula is C
12h
13nO
2, platymiscium growth regulator, it is mainly used in rooting of cuttings, can induce the formation of root substance, promotes Cell Differentiation and division, is conducive to the growth of new root and the differentiation of fibrovascular system, promotes the formation of cutting adventive root.Indolebutyric acid promotes that the effect of plant root of hair is not only significantly better than heteroauxin, and stable in properties, not easily conduct in vivo, only rest on treatment sites, use safer.By above two kinds of principal components are carried out composite, effectively can not only utilize the technical advantage of each composition, formed and have complementary advantages, and can avoid that single component concentration is higher can suppress the side effect of aerial growth and concentration is lower to inspiring the unconspicuous difficult problem of root growth; Again by using talcum powder to make auxiliary agent, making solid-state pulvis of taking root of the present invention can dilute evenly, organic solvent can be avoided again the injury of root.
Above-mentioned cuttage breeding method, preferably, the compound method of described compound pulvis of taking root comprises the following steps:
1) get the methyl α-naphthyl acetate of described mass parts, use a small amount of anhydrous alcohol solution, then add a certain amount of talcum powder and the ethanolic solution of itself and methyl α-naphthyl acetate is fully mixed thoroughly, naturally dry in the shade, ethanol is wherein volatilized completely, then deposits in the containers for future use of lucifuge, obtain pulvis A;
2) get the indolebutyric acid of described mass parts, use a small amount of anhydrous alcohol solution, the talcum powder then adding residual mass part makes the ethanolic solution of itself and indolebutyric acid fully mix thoroughly, naturally dry in the shade, ethanol is wherein volatilized completely, then deposits in the containers for future use of lucifuge, obtain pulvis B;
3) the pulvis A and the pulvis B that get respective quality ratio mix, and obtain described compound pulvis of taking root.
In the compound method of the invention described above, because formula material is insoluble in water, be soluble in organic solvent, so can dissolve fully with a small amount of absolute ethyl alcohol, mix thoroughly with talcum powder and naturally to dry in the shade afterwards, ethanol is volatilized completely, because absolute ethyl alcohol has injury effect to branch and root, therefore, it is thorough that this collocation method is all considered from the utilization of action principle and active principle, the effect reached.
Above-mentioned cuttage breeding method, preferred, described step 1) in talcous consumption and described step 2) in talcous consumption substantially identical.
Above-mentioned cuttage breeding method, preferably, when carrying out cuttage to blueberry, now getting the described pulvis A of equal quality and pulvis B and putting into container and fully mix thoroughly, controlling every thousand strain cuttings and use pulvis A and each 30g of pulvis B; Compound pulvis of taking root after mixing thoroughly described in being stained with by cutting base portion during cuttage, then inserts matrix.
Compared with prior art, the invention has the advantages that:
1) cuttage breeding method of the present invention is simple to operate, all improves cutting time, cutting medium, water spray control measures etc. and optimizes, this make the blueberry rooting rate after cottage propagation and planting percent higher;
2) the present invention is also optimized improvement to the formula of pulvis of taking root, and significantly improves the effect after root-growing agent process blueberry branch, further increases rooting rate and the planting percent of blueberry cuttage;
3) process for preparation of the compound pulvis of taking root of the present invention is simple, and easy to operate, cost is lower, is suitable for extensive promotion and application;
4) compound pulvis of taking root of the present invention is specially adapted to variety culture and the cottage propagation of blueberry under southern climates condition.
5) compound pulvis of taking root of the present invention not only can be applicable to blueberry, also can be widely used in the plant (such as Chinese rose etc.) that other cuttages are difficult to take root, growth-promoting root successful (see Fig. 2).
Accompanying drawing explanation
In order to be illustrated more clearly in the embodiment of the present invention or technical scheme of the prior art, be briefly described to the accompanying drawing used required in embodiment or description of the prior art below, apparently, accompanying drawing in the following describes is some embodiments of the present invention, for those of ordinary skill in the art, under the prerequisite not paying creative work, other accompanying drawing can also be obtained according to these accompanying drawings.
Fig. 1 is the outward appearance photo of the compound pulvis of taking root prepared in the specific embodiment of the invention.
Fig. 2 is the rooting efficiency contrast photo after the branch of compound pulvis process cuttage of taking root in the specific embodiment of the invention, and wherein upper row's branch is that the present invention is taken root the rooting efficiency after pulvis process, and lower row's branch is the rooting efficiency after conventional root-growing agent process.
Fig. 3 is the growth result photo after the compound pulvis process cuttage blueberry of taking root prepared in the specific embodiment of the invention.
Fig. 4 is the growth result photo after the root-growing agent process cuttage blueberry of normal compound.
Fig. 5 adopts the pure perlite of the present invention as the reproductive effect photo of cutting medium.
Fig. 6 adopts the conventional peat composed of rotten mosses as the reproductive effect photo of cutting medium.
Fig. 7 adopts conventional earth as the reproductive effect photo of cutting medium.
Embodiment
For the ease of understanding the present invention, hereafter will do to describe more comprehensively, meticulously to the present invention in conjunction with Figure of description and preferred embodiment, but protection scope of the present invention is not limited to following specific embodiment.
Unless otherwise defined, hereinafter used all technical terms are identical with the implication that those skilled in the art understand usually.The object of technical term used herein just in order to describe specific embodiment is not be intended to limit the scope of the invention.
Unless otherwise specified, the various raw material, reagent, instrument and equipment etc. used in the present invention are all bought by market and are obtained or prepare by existing method.
Embodiment:
A cuttage breeding method for southern blueberry of the present invention, comprises the following steps:
A prepared by () cutting: select annual, semi-lignified in more than 3 years age blueberry tree, healthy and strong branch without damage by disease and insect, cut and put into the bucket filling water and make it not dewater, in the cool branch segmentation is cut into the cutting of 10cm ~ 12cm, and each cutting keeps more than 2 leaf buds, water container is put in cutting for subsequent use;
B () matrix prepares: inserted by cutting medium in cuttage railway carriage or compartment, the matrix of cuttage selects pure perlite, and trickle drenched leveling compacting, dries in the shade one day, naturally with 500 times of liquid carbendazim spraying disinfections before cuttage;
C () cuttage: in first arrival in August by the end of September, chooses above-mentioned cutting for subsequent use, cutting base portion is stained with pulvis of taking root, insert 3cm in matrix, and each cutting keeps the spacing of about 8cm;
(d) water spray management: water spray management is carried out to the cutting after above-mentioned cuttage, adopt thin spray pattern to spray water and (must guard against pouring, spraying can adopt agroatomizer or automatic spraying system), according to temperature difference adjustment day spray timings, at least sprayed 1 time every 2 hours without during rain megathermal climate; Temperature, 25 DEG C ~ 30 DEG C time, was at least sprayed 1 time every 4 hours; Each spraying duration controls at 10s ~ 30s, and keep canopy humidity 80% ~ 90%, soil humidity is 65% ~ 75%, continue at least 20 days, when cutting occurs that withered and yellow, the new leaf bud of Lao Ye part starts to sprout, cutting base portion starts to bear callus, gradually reduce a day spray timings, keep matrix see dry see wet, until cuttage after at least 50 days cutting basic (at least 80% ~ 90%) grow new root, and new branches and leaves grow fine.
The pulvis of taking root used in above-mentioned the present embodiment is compound pulvis of taking root, as shown in Figure 1, its be by mass ratio be 1: 1 pulvis A and pulvis B compound mix;
Pulvis A is formulated primarily of following mass fraction component:
Methyl α-naphthyl acetate: 2 parts (1 ~ 2 part);
Talcum powder: 1000 parts;
Pulvis B is formulated primarily of following mass fraction component:
Indolebutyric acid: 1 part;
Talcum powder: 1000 parts, talcum powder is 1200 object industrial talc powder.
The compound method of the above-mentioned compound pulvis of taking root of the present embodiment, comprises the following steps:
1) get 1g methyl α-naphthyl acetate, use a small amount of anhydrous alcohol solution, the talcum powder then adding 500g makes the ethanolic solution of itself and methyl α-naphthyl acetate fully mix thoroughly, naturally dries in the shade, ethanol is wherein volatilized completely, then deposits in the containers for future use of lucifuge, obtain pulvis A;
2) get 0.5g indolebutyric acid, use a small amount of anhydrous alcohol solution, the talcum powder then adding 500g makes the ethanolic solution of itself and indolebutyric acid fully mix thoroughly, naturally dries in the shade, ethanol is wherein volatilized completely, then deposits in the containers for future use of lucifuge, obtain pulvis B;
3) get mass ratio be 1: 1 pulvis A and pulvis B mix, obtain compound pulvis of taking root.
When carrying out cuttage to blueberry, now getting the pulvis A of homogenous quantities and pulvis B and putting into container and fully mix thoroughly, controlling every thousand strain cuttings and use pulvis A and each 30g of pulvis B; Compound pulvis of taking root after mixing thoroughly described in being stained with by cutting base portion during cuttage, then inserts matrix.
Cottage propagation Experimental Comparison is done with conventional methyl α-naphthyl acetate root-growing agent B (concentration is 2000 ~ 3000ml/l) with the compound pulvis A of taking root that above-mentioned the present embodiment is prepared, after blueberry branch with the compound pulvis A process cuttage of taking root of the present embodiment, the high clump blueberry kind rooting rate in south reaches more than 90%, and planting percent reaches more than 80%; Vaccinium ashei cutting plantation is more than 80%, and planting percent reaches 70%; Compared with the methyl α-naphthyl acetate root-growing agent B of routine, blueberry cutting plantation of the present invention improves 40%, and planting percent improves the effect (comparative trial effect sees the following form 1) of 35%.Fig. 3 gives the growth result photo after the compound pulvis process cuttage blueberry of taking root of preparation, Fig. 4 is the growth result photo after the root-growing agent process cuttage blueberry of normal compound, comparison diagram 3 and Fig. 4 visible, blueberry grow prolifically after process of the present invention, rooting rate and planting percent significantly improve, blueberry growth after conventional root-growing agent process is sparse, and planting percent is relatively not high.
Table 1: the comparative trial effect of compound take root pulvis A and the conventional root-growing agent B of the present embodiment preparation
Table note: in upper table 1, each kind and each process test strain number are 500 strains.
Through us comparative trial repeatedly and field experiment, there is very big difference in the south cutting time of blueberry and the blueberry cultivation in other places, in the present invention, time of southern area cuttage blueberry with late summer to autumn for the best, as shown in the result of the test in following table 2, be particularly preferably the first arrival in August mentioned in above-described embodiment by the end of September, because the environmental condition such as temperature now, humidity, illumination is the most suitable, rooting rate and planting percent high, this not only effectively reduces material and cost of labor, and convenient management, be suitable for promoting.
Table 2: the comparative test result of blueberry cutting time of the present invention
Table note: in upper table 2, each kind and each process test strain number are 500 strains.
Through us comparative trial repeatedly and field experiment, we also find that different cutting mediums also can obviously have influence on cottage propagation effect, mostly use the composition of various complexity as cutting medium in prior art, traditional thinking also thinks that cutting medium composition is more, nutritive validity is better, actually this is not so, as shown in the result of the test in following table 3, different cutting mediums has respective pluses and minuses, a lot of cutting medium also can introduce various antagonism composition and pathogenic bacteria, therefore pure perlite is particularly preferably used in the present invention as cutting medium to strengthen the cottage propagation effect of blueberry further.As shown in Fig. 5 ~ Fig. 7, pure perlite in the present invention, is adopted to show best reproductive effect as the blueberry of cutting medium.
Table 3: the comparative test result of blueberry cuttage different substrates of the present invention
Table note: in upper table 3, each kind and each process test strain number are 500 strains.
Claims (10)
1. a cuttage breeding method for southern blueberry, comprises the following steps:
A prepared by () cutting: select annual, semi-lignified in more than 3 years age blueberry tree, healthy and strong branch without damage by disease and insect, cut and put into the bucket filling water and make it not dewater, in the cool branch segmentation is cut into the cutting of certain length, water container is put in cutting for subsequent use;
B () matrix prepares: inserted by cutting medium in cuttage railway carriage or compartment, and trickle drenched leveling compacting, dries in the shade naturally, the front spraying disinfection of cuttage;
C () cuttage: in first arrival in August by the end of September, chooses above-mentioned cutting for subsequent use, cutting base portion is dipped in pulvis of taking root, insert in matrix, each cutting keeps a determining deviation;
(d) water spray management: water spray management is carried out to the cutting after above-mentioned cuttage, thin spray pattern is adopted to spray water, according to temperature difference adjustment day spray timings, and keep canopy humidity 80% ~ 90%, soil humidity is 65% ~ 75%, continue at least 20 days, when cutting base portion starts to bear callus, gradually reduce a day spray timings, keep matrix see dry see wet, until cuttage after at least 50 days cutting substantially grow new root, and new branches and leaves grow fine.
2. cuttage breeding method according to claim 1, is characterized in that: in described step (a), and the cutting cut to lengthen be cut at 10cm ~ 12cm, and each cutting keeps more than 2 leaf buds.
3. cuttage breeding method according to claim 1, is characterized in that: in described step (b), and the matrix of described cuttage selects pure perlite, and spraying disinfection selects 500 times of liquid carbendazim.
4. cuttage breeding method according to claim 1, is characterized in that: in described step (c), and insert the degree of depth at least 3cm of matrix, the spacing of each cutting remains on 6cm ~ 10cm, shelters from heat or light.
5. cuttage breeding method according to claim 1, is characterized in that: in described step (d), refers to: at least sprayed 1 time every 2 hours without during rain megathermal climate according to temperature difference adjustment day spray timings; Temperature, 25 DEG C ~ 30 DEG C time, was at least sprayed 1 time every 4 hours; Each spraying duration controls at 10s ~ 30s.
6. the cuttage breeding method according to any one of Claims 1 to 5, is characterized in that: described in pulvis of taking root be compound pulvis of taking root, described compound pulvis of taking root is mixed by pulvis A and pulvis B compound;
Described pulvis A is formulated primarily of following mass fraction component:
Methyl α-naphthyl acetate: 1 ~ 2 part;
Talcum powder: 1000 parts;
Described pulvis B is formulated primarily of following mass fraction component:
Indolebutyric acid: 0.5 ~ 1 part;
Talcum powder: 1000 parts.
7. cuttage breeding method according to claim 6, is characterized in that: mass ratio when described pulvis A and pulvis B mixes is 2 ~ 1: 1; Talcum powder is 1200 object industrial talc powder.
8. cuttage breeding method according to claim 7, is characterized in that: the compound method of described compound pulvis of taking root comprises the following steps:
1) get the methyl α-naphthyl acetate of described mass parts, use a small amount of anhydrous alcohol solution, then add a certain amount of talcum powder and the ethanolic solution of itself and methyl α-naphthyl acetate is fully mixed thoroughly, naturally dry in the shade, ethanol is wherein volatilized completely, then deposits in the containers for future use of lucifuge, obtain pulvis A;
2) get the indolebutyric acid of described mass parts, use a small amount of anhydrous alcohol solution, the talcum powder then adding residual mass part makes the ethanolic solution of itself and indolebutyric acid fully mix thoroughly, naturally dry in the shade, ethanol is wherein volatilized completely, then deposits in the containers for future use of lucifuge, obtain pulvis B;
3) the pulvis A and the pulvis B that get respective quality ratio mix, and obtain described compound pulvis of taking root.
9. cuttage breeding method according to claim 8, is characterized in that: described step 1) in talcous consumption and described step 2) in talcous consumption substantially identical.
10. cuttage breeding method according to claim 6, is characterized in that: when carrying out cuttage to blueberry, now gets the described pulvis A of equal quality and pulvis B and puts into container and fully mix thoroughly, control every thousand strain cuttings and use pulvis A and each 30g ~ 45g of pulvis B; Compound pulvis of taking root after mixing thoroughly described in being stained with by cutting base portion during cuttage, then inserts matrix.
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| CN104756724A (en) * | 2015-04-15 | 2015-07-08 | 四川培培生态农业有限公司 | Blueberry green branch cutting and rooting method |
| CN105075633A (en) * | 2015-09-06 | 2015-11-25 | 李兰瑞 | Blueberry hardwood cuttage operation process |
| CN105284388A (en) * | 2015-11-18 | 2016-02-03 | 四川农业大学 | Blueberry softwood cutting seedling method |
| CN105706696A (en) * | 2016-02-29 | 2016-06-29 | 广西壮族自治区药用植物园 | Murdannia bracteata fast reproduction method |
| CN105766458A (en) * | 2016-03-14 | 2016-07-20 | 湖北华饴生物科技有限公司 | Ampelopsin grossedentata light matrix seedling tray cuttage seedling method |
| CN105917932A (en) * | 2016-05-12 | 2016-09-07 | 山东省林业科学研究院 | Method for industrialized cutting seedling culture of Pteroceltis tatarinowii Maxim |
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| CN107409737A (en) * | 2017-09-13 | 2017-12-01 | 四川省农业科学院园艺研究所 | A kind of container culturing method of the organic high yield of blueberry |
| CN115997786A (en) * | 2023-02-07 | 2023-04-25 | 江苏省有色金属华东地质勘查局地球化学勘查与海洋地质调查研究院 | Growth regulator for improving strontium content of blueberry fruits |
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| CN104756724A (en) * | 2015-04-15 | 2015-07-08 | 四川培培生态农业有限公司 | Blueberry green branch cutting and rooting method |
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| CN105075633A (en) * | 2015-09-06 | 2015-11-25 | 李兰瑞 | Blueberry hardwood cuttage operation process |
| CN105284388A (en) * | 2015-11-18 | 2016-02-03 | 四川农业大学 | Blueberry softwood cutting seedling method |
| CN105706696A (en) * | 2016-02-29 | 2016-06-29 | 广西壮族自治区药用植物园 | Murdannia bracteata fast reproduction method |
| CN105766458A (en) * | 2016-03-14 | 2016-07-20 | 湖北华饴生物科技有限公司 | Ampelopsin grossedentata light matrix seedling tray cuttage seedling method |
| CN105917932A (en) * | 2016-05-12 | 2016-09-07 | 山东省林业科学研究院 | Method for industrialized cutting seedling culture of Pteroceltis tatarinowii Maxim |
| CN107318647A (en) * | 2017-06-06 | 2017-11-07 | 山东大丰园农业有限公司 | A kind of root-growing agent and its outside sprout-cultivating-bottle method for blueberry tissue culture outside sprout-cultivating-bottle radication |
| CN107409737A (en) * | 2017-09-13 | 2017-12-01 | 四川省农业科学院园艺研究所 | A kind of container culturing method of the organic high yield of blueberry |
| CN115997786A (en) * | 2023-02-07 | 2023-04-25 | 江苏省有色金属华东地质勘查局地球化学勘查与海洋地质调查研究院 | Growth regulator for improving strontium content of blueberry fruits |
| CN115997786B (en) * | 2023-02-07 | 2024-05-07 | 江苏省有色金属华东地质勘查局地球化学勘查与海洋地质调查研究院 | Growth regulator for improving strontium content of blueberry fruits |
| CN116267264A (en) * | 2023-03-17 | 2023-06-23 | 贵州省亚热带作物研究所 | Raspberry cutting seedling raising method |
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