CN104756724A - Blueberry green branch cutting and rooting method - Google Patents
Blueberry green branch cutting and rooting method Download PDFInfo
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- CN104756724A CN104756724A CN201510177064.3A CN201510177064A CN104756724A CN 104756724 A CN104756724 A CN 104756724A CN 201510177064 A CN201510177064 A CN 201510177064A CN 104756724 A CN104756724 A CN 104756724A
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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Abstract
The invention discloses a blueberry green branch cutting and rooting method which includes the following steps of S1, seedbed building, S2, substrate processing, S3, ear picking, S4, cutting slips preparing, S5, cutting slips disinfection, S6, hormone processing, S7, green branch cutting and S8, post-cutting management. The blueberry green branch cutting and rooting method has the advantages that by means of the blueberry green branch cutting and rooting method, the rooting rate is high and can reach 80%, and the transplant survival rate exceeds 90%; an efficient blueberry green branch cutting and rooting system is built, the method has the advantages of being easy to operate, low in cost, high in rooting speed and high in stability, and large-scale factory-like blueberry seedling breeding is facilitated.
Description
Technical field
The invention belongs to technical field of plant asexual propagation, be specifically related to a kind of blueberry green wood cutting rooting method.
Background technology
Blueberry (Vaccinium corymbosum) is Ericaceae Vaccinium plant, for perennial fallen leaves or evergreen shrubs, fruit is berry, be that a kind of value first grown up in the U.S. is high, remarkable in economical benefits, there is the nutrition and health care fruit of high nutritive value, health-care effect and medical value.Blueberry is rich in the anti-oxidant compounds such as various vitamin, ursin, anthocyanin, SOD, ellagic acid and flavonoids, and therefore it has unique health care.Blueberry is also one of the most potential fruit tree species in this world wide simultaneously.
Blueberry is color very attractive not only, and meat exquisiteness, moderately sour and sweet, nutritious, fragrance are salubrious, unique flavor, both can eat raw, and also can be processed into multiple relevant food, and deeply like by consumer.It is reported, blueberry is that in all fruits and vegetables, antioxidant content content is the highest, particularly contain abundant anthocyan in fruit, to pre-preventing thrombosis and arteriosclerosis, anticancer, opposing urinary system infection contamination, preventing heart disease with delay senility all there is good effect, be one health food very reliably, therefore the international food and agricultural organization of Ye Bei is classified as one of large healthy food of the mankind five.
Last century, the eighties rose, and China from having introduced a large amount of cultivars abroad, and starts related promotional work.But the deficiency of nurseries' supplies limits the development of current area under cultivation.The progeny variation amplitude of Seedling propagation is large, can not keep the good characteristic of kind.Tissue culture propagating fund input is large, technical requirement is high, should not grasp and use.Therefore cottage propagation becomes a kind of ideal chose.But the blueberry stage of taking root reported in existing document has the problems such as rooting rate is low, cycle length of taking root, program of taking root complexity and hardening survival rate are low, there is larger difference in the aspects such as the matrix of particularly urging root to select, the control of environment, makes it large-scale industrializedly produce.
Summary of the invention
Namely object of the present invention is to overcome the deficiencies in the prior art, provides a kind of simple to operate, with low cost, rooting rate is fast, stability is high blueberry green wood cutting rooting method.
The object of the invention is to be achieved through the following technical solutions: a kind of blueberry green wood cutting rooting method, it comprises the following steps:
S1. seedbed is built: seedbed is located in booth, seedbed length 900cm, width 80 ~ 150cm, height 25 ~ 35cm, steel wire is equipped with bottom seedbed, distance apart from ground is 3 ~ 6cm, steel wire first spreads the blinding that a layer thickness is 5 ~ 7cm, repaving the sand that a layer thickness is 5 ~ 7cm, is finally the seedling medium of 11 ~ 14cm at sand upper berth thickness; Before cuttage, with water, seedbed is irrigated;
S2. matrix treatments: above-mentioned seedling medium clear water is rinsed 2 ~ 4 times, with the tpns of 900 ~ 1200 times or 50% thiophanate methyl 700 ~ 900 times spraying disinfection is carried out to matrix after, vexed canopy tans by the sun 4 ~ 7d use;
S3. adopt fringe: in the cloudy day or morning dew dry after carried out adopting fringe before the temperature rises, choose the blueberry elite stand of robust growth, raw rugosity is 0.2 ~ 0.4cm semi-lignified young sprout to clip is then fringe bar, is adopting in fringe process, is carrying out the moisturizing of discontinuity water spray to the fringe bar gathered;
S4. prepare cuttings: rinsed 2 ~ 4 times by the fringe bar clear water gathered, be cut into that length is 10 ~ 15cm, short fringe containing 6 ~ 8, joint with scissors, the top clip of cuttings is truncate, bottom clip 42 ~ 47 °, apart from joint 0.1cm; Cuttings lower blade all cuts off, and top retains 2 ~ 3, blade, the cuttings made is put into clear water or water spray moisturizing;
S5. cuttings sterilization: the thiophanate methyl with 50% 800 ~ 1200 times soaks the two ends of cuttings, and soak time is 15 ~ 30s;
S6. HORMONE TREATMENT: sterile for step S5 cuttings base portion is immersed in 25 ~ 35min in root-growing agent;
S7. green wood cutting: the blueberry cuttings after HORMONE TREATMENT is inserted in matrix, cuttage spacing in the rows 5cm, line-spacing 5cm, cuttage degree of depth 6cm; During cuttage, medium of seedling bed humid control is 70 ~ 80%;
S8. management after inserting: adopt full-automatic spraying system, during cuttage root-taking, keeps relative air humidity 75%, air themperature 25 ~ 30 DEG C, daylight intensity >=900LX.
Further, in described step S1, seedling medium is the mixture of perlite and liver moss, and mixed proportion is 1 ~ 1.5:1, and regulates the pH of matrix to be 4.5 ~ 5.5 with phosphate buffer.
Further, in described step S6, root-growing agent is the mixed solution of methyl α-naphthyl acetate, indolebutyric acid, vitamin B1,6-BA, p-chlorophenoxyacetic acid, vitamin B6,95% ethanol, and concentration is respectively: 200 ㎎/L, 200 ㎎/L, 20 ㎎/L, 10 ㎎/L, 200 ㎎/L, 20 ㎎/L, 13.3 ㎎/L.
The invention has the beneficial effects as follows: blueberry green wood cutting rooting method of the present invention, rooting rate is high, can reach 80%, and transplanting survival rate has exceeded 90%; The method establishes a kind of blueberry green wood cutting efficiently and to take root system, has simple to operate, with low cost, that rooting rate is fast, stability is high advantage, is convenient to large-scale industrializedly breed blueberry seedling.
Embodiment
Below in conjunction with embodiment, the present invention will be further described, and protection scope of the present invention is not limited to the following stated:
embodiment 1:a kind of blueberry green wood cutting rooting method, it comprises the following steps:
S1. seedbed is built: seedbed is located in booth, seedbed length 900cm, width 80cm, height 25cm, steel wire is equipped with bottom seedbed, distance apart from ground is 3cm, steel wire first spreads the blinding that a layer thickness is 5cm, repaving the sand that a layer thickness is 5cm, is finally the seedling medium of 11cm at sand upper berth thickness; Before cuttage, with water, seedbed is irrigated; Wherein, described seedling medium is the mixture of perlite and liver moss, and mixed proportion is 1:1, and regulates the pH of matrix to be 4.5 with phosphate buffer;
S2. matrix treatments: above-mentioned seedling medium clear water is rinsed 2 times, after carrying out spraying disinfection with the tpns of 900 times to matrix, vexed canopy uses after tanning by the sun 4d;
S3. adopt fringe: in the cloudy day or morning dew dry after carried out adopting fringe before the temperature rises, choose the blueberry elite stand of robust growth, raw rugosity is 0.2cm semi-lignified young sprout to clip is then fringe bar, is adopting in fringe process, is carrying out the moisturizing of discontinuity water spray to the fringe bar gathered;
S4. prepare cuttings: the fringe bar clear water of collection is rinsed 2 times, be cut into that length is 10cm, short fringe containing 6, joint with scissors, the top clip of cuttings is truncate, bottom clip 42 °, apart from joint 0.1cm; Cuttings lower blade all cuts off, and top retains 2, blade, the cuttings made is put into clear water or water spray moisturizing;
S5. cuttings sterilization: the thiophanate methyl with 50% 800 times soaks the two ends of cuttings, and soak time is 15s;
S6. HORMONE TREATMENT: sterile for step S5 cuttings base portion is immersed in 25min in root-growing agent; Wherein, described root-growing agent is the mixed solution of methyl α-naphthyl acetate, indolebutyric acid, vitamin B1,6-BA, p-chlorophenoxyacetic acid, vitamin B6,95% ethanol, and concentration is respectively: 200 ㎎/L, 200 ㎎/L, 20 ㎎/L, 10 ㎎/L, 200 ㎎/L, 20 ㎎/L, 13.3 ㎎/L;
S7. green wood cutting: the blueberry cuttings after HORMONE TREATMENT is inserted in matrix, cuttage spacing in the rows 5cm, line-spacing 5cm, cuttage degree of depth 6cm; During cuttage, medium of seedling bed humid control is 70%;
S8. management after inserting: adopt full-automatic spraying system, during cuttage root-taking, keeps relative air humidity 75%, air themperature 25 DEG C, daylight intensity to be 900LX.
embodiment 2:a kind of blueberry green wood cutting rooting method, it comprises the following steps:
S1. seedbed is built: seedbed is located in booth, seedbed length 900cm, width 150cm, height 35cm, steel wire is equipped with bottom seedbed, distance apart from ground is 6cm, steel wire first spreads the blinding that a layer thickness is 7cm, repaving the sand that a layer thickness is 7cm, is finally the seedling medium of 14cm at sand upper berth thickness; Before cuttage, with water, seedbed is irrigated; Wherein, described seedling medium is the mixture of perlite and liver moss, and mixed proportion is 1.5:1, and regulates the pH of matrix to be 5.5 with phosphate buffer;
S2. matrix treatments: above-mentioned seedling medium clear water is rinsed 4 times, after carrying out spraying disinfection with the tpns of 1200 times to matrix, vexed canopy uses after tanning by the sun 7d;
S3. adopt fringe: in the cloudy day or morning dew dry after carried out adopting fringe before the temperature rises, choose the blueberry elite stand of robust growth, raw rugosity is 0.4cm semi-lignified young sprout to clip is then fringe bar, is adopting in fringe process, is carrying out the moisturizing of discontinuity water spray to the fringe bar gathered;
S4. prepare cuttings: the fringe bar clear water of collection is rinsed 4 times, be cut into that length is 15cm, short fringe containing 8, joint with scissors, the top clip of cuttings is truncate, bottom clip 47 °, apart from joint 0.1cm; Cuttings lower blade all cuts off, and top retains 3, blade, the cuttings made is put into clear water or water spray moisturizing;
S5. cuttings sterilization: the thiophanate methyl with 50% 1200 times soaks the two ends of cuttings, and soak time is 30s;
S6. HORMONE TREATMENT: sterile for step S5 cuttings base portion is immersed in 35min in root-growing agent; Wherein, institute's root-growing agent is the mixed solution of methyl α-naphthyl acetate, indolebutyric acid, vitamin B1,6-BA, p-chlorophenoxyacetic acid, vitamin B6,95% ethanol, and concentration is respectively: 200 ㎎/L, 200 ㎎/L, 20 ㎎/L, 10 ㎎/L, 200 ㎎/L, 20 ㎎/L, 13.3 ㎎/L;
S7. green wood cutting: the blueberry cuttings after HORMONE TREATMENT is inserted in matrix, cuttage spacing in the rows 5cm, line-spacing 5cm, cuttage degree of depth 6cm; During cuttage, medium of seedling bed humid control is 80%;
S8. management after inserting: adopt full-automatic spraying system, during cuttage root-taking, keeps relative air humidity 75%, air themperature 30 DEG C, daylight intensity 1000LX.
embodiment 3:a kind of blueberry green wood cutting rooting method, it comprises the following steps:
S1. seedbed is built: seedbed is located in booth, seedbed length 900cm, width 100cm, height 30cm, steel wire is equipped with bottom seedbed, distance apart from ground is 4cm, steel wire first spreads the blinding that a layer thickness is 6cm, repaving the sand that a layer thickness is 6cm, is finally the seedling medium of 12cm at sand upper berth thickness; Before cuttage, with water, seedbed is irrigated; Wherein, described seedling medium is the mixture of perlite and liver moss, and mixed proportion is 1.2:1, and regulates the pH of matrix to be 5 with phosphate buffer;
S2. matrix treatments: above-mentioned seedling medium clear water is rinsed 3 times, after carrying out spraying disinfection with the tpns of 1000 times to matrix, vexed canopy uses after tanning by the sun 5d;
S3. adopt fringe: in the cloudy day or morning dew dry after carried out adopting fringe before the temperature rises, choose the blueberry elite stand of robust growth, raw rugosity is 0.3cm semi-lignified young sprout to clip is then fringe bar, is adopting in fringe process, is carrying out the moisturizing of discontinuity water spray to the fringe bar gathered;
S4. prepare cuttings: the fringe bar clear water of collection is rinsed 3 times, be cut into that length is 12cm, short fringe containing 7, joint with scissors, the top clip of cuttings is truncate, bottom clip 45 °, apart from joint 0.1cm; Cuttings lower blade all cuts off, and top retains 3, blade, the cuttings made is put into clear water or water spray moisturizing;
S5. cuttings sterilization: the thiophanate methyl with 50% 1100 times soaks the two ends of cuttings, and soak time is 25s;
S6. HORMONE TREATMENT: sterile for step S5 cuttings base portion is immersed in 32min in root-growing agent; Wherein, described root-growing agent is the mixed solution of methyl α-naphthyl acetate, indolebutyric acid, vitamin B1,6-BA, p-chlorophenoxyacetic acid, vitamin B6,95% ethanol, and concentration is respectively: 200 ㎎/L, 200 ㎎/L, 20 ㎎/L, 10 ㎎/L, 200 ㎎/L, 20 ㎎/L, 13.3 ㎎/L;
S7. green wood cutting: the blueberry cuttings after HORMONE TREATMENT is inserted in matrix, cuttage spacing in the rows 5cm, line-spacing 5cm, cuttage degree of depth 6cm; During cuttage, medium of seedling bed humid control is 78%;
S8. management after inserting: adopt full-automatic spraying system, during cuttage root-taking, keeps relative air humidity 75%, air themperature 26 DEG C, daylight intensity 1200LX.
embodiment 4:a kind of blueberry green wood cutting rooting method, it comprises the following steps:
S1. seedbed is built: seedbed is located in booth, seedbed length 900cm, width 140cm, height 26cm, steel wire is equipped with bottom seedbed, distance apart from ground is 5cm, steel wire first spreads the blinding that a layer thickness is 7cm, repaving the sand that a layer thickness is 5cm, is finally the seedling medium of 12cm at sand upper berth thickness; Before cuttage, with water, seedbed is irrigated; Wherein, described seedling medium is the mixture of perlite and liver moss, and mixed proportion is 1.4:1, and regulates the pH of matrix to be 4.8 with phosphate buffer;
S2. matrix treatments: above-mentioned seedling medium clear water is rinsed 3 times, after the thiophanate methyl 700 with 50% doubly carries out spraying disinfection to matrix, vexed canopy uses after tanning by the sun 7d;
S3. adopt fringe: in the cloudy day or morning dew dry after carried out adopting fringe before the temperature rises, choose the blueberry elite stand of robust growth, raw rugosity is 0.3cm semi-lignified young sprout to clip is then fringe bar, is adopting in fringe process, is carrying out the moisturizing of discontinuity water spray to the fringe bar gathered;
S4. prepare cuttings: the fringe bar clear water of collection is rinsed 3 times, be cut into that length is 10cm, short fringe containing 7, joint with scissors, the top clip of cuttings is truncate, bottom clip 46 °, apart from joint 0.1cm; Cuttings lower blade all cuts off, and top retains 2, blade, the cuttings made is put into clear water or water spray moisturizing;
S5. cuttings sterilization: the thiophanate methyl with 50% 800 times soaks the two ends of cuttings, and soak time is 26s;
S6. HORMONE TREATMENT: sterile for step S5 cuttings base portion is immersed in 32min in root-growing agent; Wherein, described root-growing agent is the mixed solution of methyl α-naphthyl acetate, indolebutyric acid, vitamin B1,6-BA, p-chlorophenoxyacetic acid, vitamin B6,95% ethanol, and concentration is respectively: 200 ㎎/L, 200 ㎎/L, 20 ㎎/L, 10 ㎎/L, 200 ㎎/L, 20 ㎎/L, 13.3 ㎎/L;
S7. green wood cutting: the blueberry cuttings after HORMONE TREATMENT is inserted in matrix, cuttage spacing in the rows 5cm, line-spacing 5cm, cuttage degree of depth 6cm; During cuttage, medium of seedling bed humid control is 80%;
S8. management after inserting: adopt full-automatic spraying system, during cuttage root-taking, keeps relative air humidity 75%, air themperature 25 DEG C, daylight intensity 1500LX.
embodiment 5:a kind of blueberry green wood cutting rooting method, it comprises the following steps:
S1. seedbed is built: seedbed is located in booth, seedbed length 900cm, width 105cm, height 27cm, steel wire is equipped with bottom seedbed, distance apart from ground is 6cm, steel wire first spreads the blinding that a layer thickness is 5cm, repaving the sand that a layer thickness is 7cm, is finally the seedling medium of 12cm at sand upper berth thickness; Before cuttage, with water, seedbed is irrigated; Wherein, described seedling medium is the mixture of perlite and liver moss, and mixed proportion is 1.5:1, and regulates the pH of matrix to be 4.7 with phosphate buffer;
S2. matrix treatments: above-mentioned seedling medium clear water is rinsed 4 times, after the thiophanate methyl 900 with 50% doubly carries out spraying disinfection to matrix, vexed canopy uses after tanning by the sun 5d;
S3. adopt fringe: in the cloudy day or morning dew dry after carried out adopting fringe before the temperature rises, choose the blueberry elite stand of robust growth, raw rugosity is 0.3cm semi-lignified young sprout to clip is then fringe bar, is adopting in fringe process, is carrying out the moisturizing of discontinuity water spray to the fringe bar gathered;
S4. prepare cuttings: the fringe bar clear water of collection is rinsed 3 times, be cut into that length is 13cm, short fringe containing 6, joint with scissors, the top clip of cuttings is truncate, bottom clip 47 °, apart from joint 0.1cm; Cuttings lower blade all cuts off, and top retains 2, blade, the cuttings made is put into clear water or water spray moisturizing;
S5. cuttings sterilization: the thiophanate methyl with 50% 115 times soaks the two ends of cuttings, and soak time is 22s;
S6. HORMONE TREATMENT: sterile for step S5 cuttings base portion is immersed in 25min in root-growing agent; Wherein, described root-growing agent is the mixed solution of methyl α-naphthyl acetate, indolebutyric acid, vitamin B1,6-BA, p-chlorophenoxyacetic acid, vitamin B6,95% ethanol, and concentration is respectively: 200 ㎎/L, 200 ㎎/L, 20 ㎎/L, 10 ㎎/L, 200 ㎎/L, 20 ㎎/L, 13.3 ㎎/L;
S7. green wood cutting: the blueberry cuttings after HORMONE TREATMENT is inserted in matrix, cuttage spacing in the rows 5cm, line-spacing 5cm, cuttage degree of depth 6cm; During cuttage, medium of seedling bed humid control is 75%;
S8. management after inserting: adopt full-automatic spraying system, during cuttage root-taking, keeps relative air humidity 75%, air themperature 25 DEG C, daylight intensity 2000LX.
Claims (3)
1. a blueberry green wood cutting rooting method, is characterized in that, it comprises the following steps:
S1. seedbed is built: seedbed is located in booth, seedbed length 900cm, width 80 ~ 150cm, height 25 ~ 35cm, steel wire is equipped with bottom seedbed, distance apart from ground is 3 ~ 6cm, steel wire first spreads the blinding that a layer thickness is 5 ~ 7cm, repaving the sand that a layer thickness is 5 ~ 7cm, is finally the seedling medium of 11 ~ 14cm at sand upper berth thickness; Before cuttage, with water, seedbed is irrigated;
S2. matrix treatments: above-mentioned seedling medium clear water is rinsed 2 ~ 4 times, with the tpns of 900 ~ 1200 times or 50% thiophanate methyl 700 ~ 900 times spraying disinfection is carried out to matrix after, vexed canopy tans by the sun 4 ~ 7d use;
S3. adopt fringe: in the cloudy day or morning dew dry after carried out adopting fringe before the temperature rises, choose the blueberry elite stand of robust growth, raw rugosity is 0.2 ~ 0.4cm semi-lignified young sprout to clip is then fringe bar, is adopting in fringe process, is carrying out the moisturizing of discontinuity water spray to the fringe bar gathered;
S4. prepare cuttings: rinsed 2 ~ 4 times by the fringe bar clear water gathered, be cut into that length is 10 ~ 15cm, short fringe containing 6 ~ 8, joint with scissors, the top clip of cuttings is truncate, bottom clip 42 ~ 47 °, apart from joint 0.1cm; Cuttings lower blade all cuts off, and top retains 2 ~ 3, blade, the cuttings made is put into clear water or water spray moisturizing;
S5. cuttings sterilization: the thiophanate methyl with 50% 800 ~ 1200 times soaks the two ends of cuttings, and soak time is 15 ~ 30s;
S6. HORMONE TREATMENT: sterile for step S5 cuttings base portion is immersed in 25 ~ 35min in root-growing agent;
S7. green wood cutting: the blueberry cuttings after HORMONE TREATMENT is inserted in matrix, cuttage spacing in the rows 5cm, line-spacing 5cm, cuttage degree of depth 6cm; During cuttage, medium of seedling bed humid control is 70 ~ 80%;
S8. management after inserting: adopt full-automatic spraying system, during cuttage root-taking, keeps relative air humidity 75%, air themperature 25 ~ 30 DEG C, daylight intensity >=900LX.
2. a kind of blueberry green wood cutting rooting method according to claim 1, it is characterized in that, in described step S1, seedling medium is the mixture of perlite and liver moss, and mixed proportion is 1 ~ 1.5:1, and regulates the pH of matrix to be 4.5 ~ 5.5 with phosphate buffer.
3. a kind of blueberry green wood cutting rooting method according to claim 1, it is characterized in that, in described step S6, root-growing agent is the mixed solution of methyl α-naphthyl acetate, indolebutyric acid, vitamin B1,6-BA, p-chlorophenoxyacetic acid, vitamin B6,95% ethanol, and concentration is respectively: 200 ㎎/L, 200 ㎎/L, 20 ㎎/L, 10 ㎎/L, 200 ㎎/L, 20 ㎎/L, 13.3 ㎎/L.
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| CN105075633A (en) * | 2015-09-06 | 2015-11-25 | 李兰瑞 | Blueberry hardwood cuttage operation process |
| CN105123378A (en) * | 2015-07-20 | 2015-12-09 | 四川省农业科学院经济作物育种栽培研究所 | Green planting technology for south open ground blueberry and interplanting winter strawberry of same |
| CN105284388A (en) * | 2015-11-18 | 2016-02-03 | 四川农业大学 | Blueberry softwood cutting seedling method |
| CN106258434A (en) * | 2016-08-19 | 2017-01-04 | 江苏艺轩园林景观工程有限公司 | Blue berry booth Seedling inserts rear management method |
| CN106818435A (en) * | 2016-12-19 | 2017-06-13 | 新昌县钧国生物技术有限公司 | A kind of taxus chinensis vegetative propagation rapidly rooting method |
| CN106942035A (en) * | 2017-04-25 | 2017-07-14 | 江西思科农业开发有限责任公司 | A kind of blueberry seed selection and mating system |
| CN108738771A (en) * | 2018-05-16 | 2018-11-06 | 苏州御湖园农业科技有限公司 | A kind of HORMONE TREATMENT measure improving blueberry cutting plantation |
| CN108886988A (en) * | 2018-06-28 | 2018-11-27 | 普定县祝春农产品有限公司 | A kind of blueberry sponge cuttage and seedling culture method |
| CN111771594A (en) * | 2020-07-15 | 2020-10-16 | 湖南省森林植物园 | Cutting propagation method for blueberries in Lykexi |
| CN115104441A (en) * | 2022-08-12 | 2022-09-27 | 河南科技大学 | Efficient rooting method for blueberry twig cuttage |
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