CN105284388A - Blueberry softwood cutting seedling method - Google Patents
Blueberry softwood cutting seedling method Download PDFInfo
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- CN105284388A CN105284388A CN201510797012.6A CN201510797012A CN105284388A CN 105284388 A CN105284388 A CN 105284388A CN 201510797012 A CN201510797012 A CN 201510797012A CN 105284388 A CN105284388 A CN 105284388A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
Abstract
The present invention discloses a blueberry softwood cutting seedling method. The method comprises the following steps: selecting and trimming cutting woods, soaking the trimmed woods with a rooting agent containing boron and IBA for 8-10 hours, and inserting the woods into a matrix, wherein the humidity of the matrix is kept at 80% or above, and the temperature is controlled at 20-28 DEG C. The method of the present invention is simple to study, high in operability, good in rooting effect and high in utility value, wherein the rooting rate reaches up to 91.18%, so that the callus formation rate and the rooting number can be significantly improved.
Description
Technical field
The invention belongs to technical field of plant asexual propagation, be specifically related to a kind of blueberry green wood cutting seedling-cultivating method.
Background technology
At present, China's blueberry plant production development is rapid, and cultivated area constantly expands, and nursery stock demand is large, and nurseries' supplies quantity not sufficient has become the bottleneck hindering China's blueberry industry development.But because blueberry is shallow root system plant, root system fiber is undeveloped, unrooted hair, comparatively harsh to conditional requests such as soil pH value, soil moisture, gas permeability, drainage, weather conditions, management methods, so it is extremely difficult to take root.
Current Domestic mainly adopts tissue culture technology to carry out the Fast-propagation of blueberry, but some microorganisms are often carried in explant surface and inside.Simultaneously, the growth of the suitable micro-organisms such as the temperature in plant tissue culture course, humidity, nutrition, pH value, once microorganism enters Fast-propagation in culture vessel, make vegetable material generation disease or death by approach such as nutrient competition, erosion vegetable material, secretion toxic metabolic products, cause the failure of tissue cultures.Pollute in tissue cultures be and general, because root induction is the bottleneck in blueberry tissue culture work, limit the utilization of this technology in commodity production to a great extent.Therefore, correct cottage method is selected to be only the important means of the quick growth and breeding of blueberry, guarantee kind merit.
In production, the fast breeding of blueberry mainly adopts the mode of tissue culture propagation, small part adopts the hard branch of blueberry, green wood cutting technology, but mainly concentrate on being used alone of various exogenous hormone (IAA, IBA etc.), blueberry spray rooting of cuttings is promoted with this, but rooting rate is lower, rooting efficiency is undesirable, and the market demand that these technology still can not meet blueberry large-scale breeding is carried out.
Summary of the invention
The object of the present invention is to provide a kind of blueberry green wood cutting seedling-cultivating method; by adding boron, sucrose, the Cobastab of variable concentrations in IBA; application puies forward blueberry cuttage survival rate, for blueberry scale cutting propagation provides new theory and practice foundation further from the root-growing agent made.
The present invention realizes especially by following technical scheme:
A kind of blueberry green wood cutting seedling-cultivating method, specifically comprises the following steps:
1) selection of cuttings: in 3 ~ April, choose that 1 year stiff degree is large, maturity good and the position, vegetative shoot middle and lower part of health as cuttings;
2) process of cuttings: branch is pruned growth 7 ~ 10cm, is with the cuttings of 4 ~ 5 axillalry buds and top 1 ~ 2 leaf, and upper clip is truncate apart from bud 1cm, lower clip 45 ° of bevels;
3) immersion of cuttings: soak 8 ~ 10h with the root-growing agent of boron+IBA;
4) cuttage: matrix is watered permeable guarantee humidity before cuttage but not ponding, cuttage, by after cutting sterilization ling more than 1000 times immersion base portion 10s of handling well, is vertically inserted in matrix by cuttage same day;
5) water management: the humidity of matrix maintains more than 80%, temperature controls at 20 ~ 28 DEG C;
6) management after cuttage: apply fertilizer gradually after cuttage rooting, applying fertilizer applies with the form of liquid fertilizer, and concentration is no more than 3%, carries out 1 fertilising weekly to the green branch of blueberry cuttage.
Further,
The aseptic water as solvent of root-growing agent described in step (3), preparation root-growing agent, ensure that IBA concentration is 50mg/L, the concentration of boron is 300mg/L;
Described matrix is for utilizing liver moss soil as cutting medium, and adjust ph is 5.5, and the previous day is carried out sprinkling process to slotting machine matrix 1000 times of carbendazim in cuttage;
Described cuttage is specially the degree of depth inserting matrix and accounts for 2/3 of whole cutting, and spacing is 5cm × 10cm.
Beneficial effect of the present invention is: 1) the method is through strict test, and result accurately and reliably.This technology is easy to learn, workable, and rooting efficiency is good, and using value is higher, meets the production requirement of blueberry green wood cutting; 2) rooting rate of the present invention reaches as high as 91.18%, and number of on average taking root is respectively and reaches 16.40, and average root is long is respectively 4.75, and Rooting index is 1.83, and the extremely aobvious raising of cutting enzymic activity; 3) Callus formation rate can be made to significantly improve with quantity of taking root.
Embodiment
Below in conjunction with embodiment, the present invention is described further, the following stated, only to preferred embodiment of the present invention, not do other forms of restriction to the present invention, any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed to the Equivalent embodiments of equal change.Everyly do not depart from the present invention program's content, any simple modification done following examples according to technical spirit of the present invention or equivalent variations, all drop in protection scope of the present invention.
Embodiment 1
Test is carried out in the blueberry Demonstration Base green house of Baima town, Meishan city Gong Cun, chooses that south, Gong Cun blueberry planting base, Baima town, Meishan City high clump blueberry two kind indigo plants are rich, the raw then green branch of Herbert robust growth.
Choose southern high clump blueberry Herbert in 3 ~ April in 2013, the raw then green branch of blue rich robust growth takes back laboratory, cutting should from robust growth, (branch hardness should be selected large without clip on the tree of damage by disease and insect, good and the branch of health of maturity, avoid selecting water sprout as far as possible, the branch that marrow is large and there is the branch of freeze injury winter), if there is virus disease to occur in orchard, getting cutting tree should at more than 15cm from disease tree, cuttage branch is preferably the vegetative shoot of life in 1 year, the position, middle and lower part of branch should be selected to carry out cuttage as far as possible, branch is trimmed to the cuttings that 7 ~ 10cm is with 4 ~ 5 axillalry buds and 1 ~ 2 leaf in top.Use aseptic water as solvent, preparation root-growing agent, ensure that IBA concentration is approximately 50mg/L, the concentration of boron is 300mg/L, blueberry Herbert, blue rich cutting insert matrix soak 8 ~ 10h in root-growing agent after, and the degree of depth accounts for 2/3 of whole cutting, and spacing is 5cm × 10cm, matrix is watered permeable guarantee humidity before cuttage but not ponding, cuttage was vertically inserted in matrix with after the immersion of sterilization ling more than 1000 base portion 10s the same day by cuttage by the cutting handled well.Adopt batch (-type) automatic sprayer often to water, keep the humidity of matrix on cutting bed to maintain more than 80%, temperature controls at 20 ~ 28 DEG C.Water again, in order to avoid hinder seedling after the water temperatures such as standing time is long in the sun, the higher Shi Ying of water temperature cool.The period of water management most critical is first arrival in May 6 the end of month, and now blade launches, but cutting is not yet taken root, and water deficient easily causes cutting dead.When top blade starts to turn green, indicate that cutting has started to take root.
Fertilizer management: cuttage, take root before do not execute any fertilizer in matrix, start after rooting of cuttings to apply fertilizer gradually, to promote seedling growth.Fertilising should apply with the form of liquid fertilizer, and concentration is about 3%, carries out 1 fertilising weekly to the green branch of blueberry cuttage.For avoiding, because fertilising injures blade, water spray process must being carried out after each fertilising, rinsed well by the fertilizer on blade face.
Pest management: main employing between nursery stage of taking root is ventilated and processed, except the method for diseased plant is to control blueberry susceptible disease.So booth or nurse young plants in hothouses all will ventilate in time, reduce fungal disease and reduce nursery indoor temperature.
Set up control group, control group is used separately exogenous hormone IBA and to be taken root nursery simultaneously.
Through applicant's statistics, compared with Sterile culture seedling-cultivating method, partial period rooting rate, take root efficiency as shown in table 1, table 2, show that making root-growing agent by oneself can improve the rooting rate of blueberry and efficiency of taking root effectively.
The impact that table 1 boron compound IBA root-growing agent was taken root on the blue Feng Butong stage of taking root
Note: efficiency Q=(P of taking root
n-P
n-1)/take root number of days (P
nfor the rooting rate of a rear rootage duration point, P
n-1rooting rate for previous rootage duration).(lower same).
The impact that table 2 boron compound IBA root-growing agent was taken root on the Herbert different stage of taking root
As shown in Table 3, 4, after two blueberry kind cuttage 70d, rooting rate, on average take root that number, average root are long, the power index of taking root respectively higher than independent IBA process, rooting efficiency is obvious.
The blue rich situation of finally taking root of table 3
Note: power of taking root index=(average root length × root quantity)/total cuttings number.(lower same).
Table 4 Herbert is finally taken root situation
By the application of this technology, substantially increase the rooting rate of blueberry green wood cutting, be conducive to the Fast-propagation of blueberry cuttage, wide market, there is larger social value and economic worth.
For further illustrating root-growing agent to the impact in the reproductive process of seedling cultivation and rooting, normal experiment is utilized to elaborate and analyze below.
1) test material
Test is carried out in the blueberry Demonstration Base green house of Baima town, Meishan city Gong Cun, chooses that south, Gong Cun blueberry planting base, Baima town, Meishan City high clump blueberry two kind indigo plants are rich, the raw then green branch of Herbert robust growth.
2) experimental field overview
In October, 2010, marching into the arena smoothly in the ecological base of Meishan blueberry that gross investment reaches 4,500 ten thousand yuan, enters in " Meishan first village "---Gong Cun.Through many taking pains to foster half a year, the blueberry planted experimentally first at Meishan succeeds.Experimental field the current scale in the ecological base of Baima town Gong Cun blueberry reaches 100 mu, and this village plants experimentally successful blueberry kind and reached 3.Experimental field have the large-scale green house for blueberry cuttage plantation, and be furnished with full-automatic sprayer unit, the environment being throughout the year beneficial to blueberry cottage propagation can be provided.
3) test apparatus
Agilent1260 liquid chromatograph; Rotary Evaporators; PH acidometer; Centrifuge; Shaking table; Thermostat water bath; Spectrophotometer; Balance; Mortar; Test tube; Liquid-transfering gun; Beaker; 0.22 μm of filter; 10mL needle tubing.
4) test drug
Hormone standard specimen IAA, ABA are provided by SIGMA company; Chromatographic grade methyl alcohol and acetic acid; Liquid nitrogen; Polyvinylpyrrolidone (PVPP); Analytically pure ethyl acetate and benzinum; Toluene di-tert-butyl phenol (BHT); Phosphate buffer (pH=6.0); Quartz sand: Dichlorophenol; Manganese chloride; Iron chloride: ethanol; The concentrated sulfuric acid; Heteroauxin reagent A (15mL0.5moIL
-1feCl
3, the 300mL concentrated sulfuric acid, 500mL distilled water.Use forward slip value, keep in Dark Place.Reagent 4mL is added) in used time 1mL sample.
5) test method
Choose southern high clump blueberry Herbert on August 23rd, 2013, the raw then green branch of blue rich robust growth takes back laboratory, branch is trimmed to the cuttings that 7 ~ 10cm is with 4 ~ 5 axillalry buds and 1 ~ 2 leaf in top.IBA is mixed with 50mgL respectively
-1, 100mgL
-1, 200mgL
-1root-growing agent, if clear water is treated to contrast totally 4 process, 3 times repeat.Each kind soaks IBA and the clear water of variable concentrations with 20 cutting a bundles, takes out a bundle (total immersion bubble 10h), adopt the cortex endogenous IAA of high effective liquid chromatography for measuring apart from cutting base portion 1 ~ 2cm scope and the content of ABA every 2 hours.With IAA/ABA the highest concentration and the time determining IBA process.
1. experimental design
Cutting time is from November 29,5 days ~ 2013 September in 2013.Cutting medium is liver moss soil, and pH value is 4.5.Branch is trimmed to the cuttings that 7 ~ 10cm is with 4 ~ 5 axillalry buds and 1 ~ 2 leaf in top, upper clip is truncate apart from bud 1cm, lower clip 45 ° of bevels.Add in Lan Feng, Herbert root-growing agent boron, sucrose, Cobastab proportioning in table 1, IBA concentration is respectively to test the highest corresponding being as the criterion of a cuttings Endogenous IAA/ABA.Each root-growing agent formula is a kind of process, often processes 70 cuttings, in triplicate.If IBA is treated to CK separately
1, independent clear water is treated to CK
2.The cuttings processing time is to test the highest corresponding being as the criterion of an endogenous hormones ratio.On September 5th, 2013 carries out cuttage, often process 7d once (i.e. cuttage 1d, 8d, 15d, 23d, 30d, 37d, 45d) randomly draw 10 stem cutting article dynamic monitoring cutting callus and to take root situation, to take root efficiency Q=(Pn-Pn-1)/number of days of taking root (Pn is the rooting rate of a rear rootage duration point, and Pn-1 is the rooting rate of previous rootage duration).Because blueberry root system is thin and delicate, root system development is slow, cutting observation and testing index will be no longer taken out after (cuttage 45d) therefore cutting is generally taken root, and 80d (cuttage complete period) carries out final assessment to result of taking root after inserting, to take root the comprehensive quality of rooting of power index assessment with root system simultaneously, power of taking root index=(average root length × root quantity)/total cuttings number.
Table 5 root-growing agent formula
2. cuttings cortex endogenous hormones extracting method
Liquid phase chromatogram condition: AgilentC18ZORBAX reverse-phase chromatographic column (150mm × 4.6mm, 5 μm); Column temperature: 35 DEG C; Wavelength: 254nm; Mobile phase is V (methyl alcohol): V (water): V (acetic acid)=45:54.4:0.6; Flow velocity: 1.0mL/min; Sample size: 20 μ l.Quantitative assay is carried out with external standard method.Endogenous hormones calibration curve is as follows:
AreaIAA＝21.254591X-9.7334905，R＝0.99390；
AreaABA＝21.0289715X-13.481436，R＝0.99393。
The extraction of endogenous hormones method is with reference to Zhang Yongzhong.From 5 days September in 2013, often process 15d once (i.e. cuttage 1d, 15d, 30d, 45d) accurately take the cortex 1g of distance cutting base portion 0 ~ 2cm scope, after adding 1gBHT, quick liquid nitrogen is milled pulverizing.Add 80% methyl alcohol 10mL of 4 DEG C of precoolings, 4 DEG C of lixiviates are spent the night, at 5000rmin
-1lower centrifugal 15min Aspirate supernatant.Residue with 80% methyl alcohol again lixiviate spend the night, merge supernatant.Supernatant is evaporated to 1/3 of original volume at 35 DEG C, collects concentrate 1molL
-1na
2hPO
4regulate pH=8.0, use isopyknic benzinum: ethyl acetate (1:1, V/V) mixed liquor extraction decolouring 3 times, discard bacteria ether phase, aqueous phase adds 0.1g polyethylene and adjoins pyrrolidone (PVPP) absorbing phenolic material.At 4 DEG C after shaking table vibration 30min, 10000rmin at 4 DEG C
-1supernatant is got after centrifugal 10min.Supernatant 2molL
-1citric acid regulates pH=3.0.The isopyknic ethyl acetate of above-mentioned solution extracts 3 times, merges fat phase.Fat decompression distillation at 35 DEG C, to below 1mL, is drawn concentrate, is repeatedly washed rotary flask on a small quantity with Chromatographic Pure Methanol, and merging concentrate and cleaning solution are settled to 10mL, cross 0.22 μm of filter membrane and detect for high performance liquid chromatography.Overall process low-temperature dark operates.
3. the mensuration of cuttings cortex indoleacetic acid oxidase
From 5 days September in 2013, often process 15d once Fresh cortex within the scope of (i.e. cuttage 1d, 15d, 30d, 45d) clip cutting base portion 0 ~ 2cm, in order to the mensuration of IAAO enzymic activity, IAAO determination of activity is modified according to the method for Zhang Zhiliang.In 1h, decompose with every milligram of protein the micrograms destroying IAA and represent enzyme activity size.
Take 1g sample, add 20mmolL
-1precooling phosphate buffer (pH=6.0) 5mL, add a small amount of quartz sand, put in ice bath and grind to form homogenate, then add the ratio of 1mL extract in 100mg Fresh Yuxincao material, dilute it with phosphate buffer, centrifugal (10000rmin
-1) 15min, get clear enzyme solution.Get 2, test tube, add manganese chloride 1mL in a test tube, Dichlorophenol 1mL, IAA2mL, enzyme liquid 1mL, phosphoric acid buffer wave 5mL, mixes.Dezymotize in another test tube outside the dense replacement of liquid phosphoric acid buffer, all the other compositions are identical, are placed in 30 DEG C of waters bath with thermostatic control together, insulation 30min.Draw reaction mixture 2mL, add heteroauxin reagent A 4mL and shake up, be placed in the dark place insulation 30min of 30 DEG C, make colour developing.The reactant liquor taken on a red color after colour developing is measured when Xi Guangshi measures in spectrophotometer and uses wavelength 530nm.Corresponding heteroauxin residual quantity is found from calibration curve according to reading.The heteroauxin amount added from the outset deducts heteroauxin amount residual after enzyme effect, to obtain decompose by enzyme the heteroauxin amount of destruction.In lh, the size destroyed heteroauxin amount (μ g) and represent enzyme activity is decomposed with every mL enzyme liquid.
Embodiment 2 difference self-control root-growing agent is on the impact of the high clump blueberry in south different take root stage rooting rate, efficiency of taking root
The high clump blueberry Herbert in south, blue Feng Butong take root stage rooting rate change as shown in table 6, table 7.As known from Table 6, Herbert kind when cuttage 23d, by 200mgL
-1its rooting rate of cuttings of boron compound IBA process is 11.67%, and pole is significantly higher than other process (P<0.01), comparatively CK
1rooting rate improve 10.00%, by CK2 process do not occur take root; When 30d, 200mgL
-1its rooting rate of boron compound IBA is still significantly higher than other process, comparatively CK
1improve 26.66%; As cuttage 37d, 300mgL
-1boron compound IBA rooting rate is the highest (with 200mgL
-1the difference of boron compound IBA process is not remarkable, is respectively 46.67% and 43.33%), comparatively CK
1rooting rate significantly improves 31.67%, CK
1rooting rate is minimum; During cuttage 45d, be all significantly higher than other respectively process (P<0.01), wherein 300mgL in pole by the rooting rate of the boron compound IBA process of variable concentrations
-1the rooting rate of boron compound IBA process is the highest, reaches 73.33%, CK
1rooting rate is minimum, is 33.33%.From different phase of taking root, Adventitious root initiation initial stage (23 ~ 30d), 200mgL
-1the efficiency pole of taking root of boron compound IBA process is significantly higher than other process (P<0.01); The Adventitious root initiation phase (30 ~ 45d), 300mgL
-1boron, 100mgL
-1the efficiency variance of taking root of boron compound IBA process is not remarkable, and all pole is significantly higher than other respectively process (P<0.01).200mgL is described
-1boron promotes that cuttage rooting concentrates on the formation initial stage (23 ~ 30d) of adventive root, and 300mgL
-1boron, 100mgL
-1boron promotes that the time that Herbert is taken root concentrates on the Adventitious root initiation phase (30 ~ 45d).
As shown in Table 7, Lan Feng when cuttage 23d, 200mgL
-1boron, 50mgL
-1the rooting of cuttings rate of Cobastab compound IBA process is 13.33%, and pole is significantly higher than other respectively process (P<0.01), CK
1rooting rate is extremely remarkable in other respectively process (P<0.01), by CK
2process do not occur take root; During cuttage 30d, 200mgL
-1boron, 50mgL
-1the rooting rate difference of Cobastab compound IBA process is not remarkable, is respectively 40% and 38.33%, and pole is significantly higher than other process (P<0.01), and 300mgL
-1the rooting rate of boron compound IBA process and CK
1what process is consistent, extremely remarkable in other respectively process (P<0.01); During cuttage 37d, 200mgL
-1boron compound IBA process rooting rate is the highest, comparatively CK
1improve 33.33%, CK
1rooting rate is minimum, is only 20%; During cuttage 45d, 300mgL
-1the rooting percent of boron compound IBA process is the highest, reaches 83.33%, is secondly 200mgL
-1boron (73.33%) and 100mgL
-1boron (63.33%) compound IBA process, and all the other respectively process all pole and are significantly higher than CK
1(P<0.01).To take root efficiency from different phase, Adventitious root initiation initial stage (23 ~ 30d), 200mgL
-1boron, 50mgL
-1the cuttage rooting of Cobastab compound IBA process is most effective, except 300mgL
-1boron Combined Processing is lower than CK
1in addition, all the other respectively process all pole and are significantly higher than CK
1(P<0.01); At Adventitious root initiation period (30 ~ 45d), 300mgL
-1the efficiency of taking root of boron compound IBA process is significantly higher than other respectively process (P<0.01), CK
1efficiency of taking root is minimum.300mgL is described
-1boron compound IBA process promotes that the time of blue Feng Shenggen concentrates on Adventitious root initiation period (30 ~ 45d), is effectively beneficial to the Rapid Rooting of cuttings.
The impact that the different root-growing agent of table 6 was taken root on the Herbert different stage of taking root
The impact that the different root-growing agent of table 7 was taken root on the blue Feng Butong stage of taking root
Embodiment 3 difference self-control root-growing agent is on the impact of the final quality of rooting of south high clump blueberry
Under different root-growing agent process, blueberry Herbert, blue rich cuttings finally take root situation as shown in table 8, table 9.As shown in Table 8, to (complete period) after Herbert kind cuttage 70d take root situation statistics show, rooting rate process from high to low successively: 5% sucrose >100mgL
-1boron >10% sucrose >200mgL
-1boron >2% sucrose >300mgL
-1boron >CK
1>50mgL
-1cobastab >200mgL
-1cobastab >100mgL
-1cobastab.Wherein the rooting rate of 5% sucrose compound IBA is the highest, reaches 91.18%, and its number of on average taking root also reaches the highest, is 16.40; 100mgL
-1the rooting rate the second of boron compound IBA, reaches 86.84%, and the 3rd by 10% sucrose Combined Processing, and rooting rate reaches 86.49%.By the cutting of the certain density IBA process of sucrose compound, general rooting rate is the highest, is secondly boron Combined Processing; And by the cutting of boron Combined Processing, its general quality of rooting is best.Therefore, in conjunction with the experimental result of dynamic rooting rate monitoring in period of taking root, the interpolation of boron can improve rooting rate fast and effectively, and comprehensive quality of rooting is higher; And sucrose can improve the efficiency of taking root of cuttings at take root the later stage (45 ~ 70d).Cobastab compound IBA process rooting efficiency is undesirable.
As shown in Table 9, situation of taking root after indigo plant rich kind cuttage 70d, rooting rate process from high to low successively: 300mgL
-1boron >100mgL
-1boron >2% sucrose >200mgL
-1boron >5% sucrose >200mgL
-1cobastab >50mgL
-1cobastab >CK
1>100mgL
-1cobastab >10% sucrose.Wherein, 300mgL
-1the rooting rate of boron compound IBA is the highest, reaches 91.18%, compares CK
1improve 33.12%, its number of on average taking root is also the highest, is 12.60; 100mgL
-1the rooting rate the second of boron compound IBA, reaches 87.50%, and the 3rd is the cutting of 2% sucrose Combined Processing, and its rooting rate reaches 85.71%.By the cutting of the certain density IBA process of boron compound, general rooting rate is the highest, and average root is long the longest, and on average take root number at most, the power index of taking root is the highest.And the DeGrain of Cobastab compound IBA process.Comprehensively take root the experimental result of dynamically observation, the interpolation of boron can improve the comprehensive quality of rooting of blue rich cuttings fast.
Table 8 Herbert is finally taken root condition survey
The blue rich condition survey of finally taking root of table 9
Under the different root-growing agent process of embodiment 4 kind Herbert, Difference of Endogenous Hormones is analyzed
Different its endogenous IAA of root-growing agent process Herbert cuttings, ABA, IAA/ABA difference analysis are as shown in table 10, table 11, table 12.As shown in Table 10, when cuttage 1d, its cortex of cuttings IAA mass fraction through with the addition of the IBA root-growing agent process of boron is significantly higher than all the other respectively process (P<0.01), and with the addition of sucrose, Cobastab the cuttings hormone-content of IBA root-growing agent process significantly lower than CK (independent IBA process).As cuttage 15d, the cuttings hormone-content that with the addition of the IBA root-growing agent process of boron is still significantly higher than all the other respectively process (P<0.01), and the cuttings IAA content that with the addition of under the IBA root-growing agent process of Cobastab is higher than CK; In the process adding sucrose, except 5% sucrose+200mgL
-1outside IBA process, 2%, 10% sucrose+200mgL
-1the lower hormone-content of IBA process is still lower than CK.During cuttage 30d, 200mgL
-1boron+200mgL
-1the cuttings IAA content of IBA process is significantly higher than all the other respectively process (P<0.01), is secondly 50mgL
-1vB+200mgL
-1iBA and 200mgL
-1vB+200mgL
-1the process of IBA.In the process adding sucrose, except 10% sucrose+200mgL
-1outside IBA process, its IAA content of the cuttings after the saccharose treatment of all the other two concentration is lower than CK.When cuttage is to 45d, allly with the addition of boron process and obtain cuttings hormone-content and be significantly higher than all the other respectively process (P<0.01), in all process, IAA content from high to low successively: 300mgL
-1boron > 100mgL
-1boron > 200mgL
-1boron > 100mgL
-1vB > 50mgL
-1vB > 200mgL
-1vB > 5% sucrose > CK > 2% sucrose > 10% sucrose.Cuttings IAA content through boron process is significantly higher than all the other respectively process (P<0.01).Found by rooting percent detection of dynamic, 200mgL
-1boron promotes that cuttage rooting concentrates on the formation initial stage (23 ~ 30d) of adventive root, and 300mgL
-1boron, 100mgL
-1boron promotes that the time that Herbert is taken root concentrates on the Adventitious root initiation phase (30 ~ 45d).The Endogenous IAA content that this and the interpolation of boron improve southern high clump blueberry cuttings Adventitious root initiation period is relevant.
The different root-growing agent of table 10 is on the impact of Herbert Endogenous IAA content
As shown in Table 11, when cuttage 1d, all its ABA content of cuttings that with the addition of the IBA root-growing agent process of boron all respectively processes higher than all the other, and the process from high to low of ABA content is followed successively by: 200mgL
-1boron > 100mgL
-1boron > 300mgL
-1boron, the lower ABA content of CK process is minimum.As cuttage 15d, its ABA content of IBA root-growing agent process cuttings that with the addition of boron still respectively processes higher than all the other, except 200mgL
-1vB+200mgL
-1beyond IBA process, all process hormone-contents are all significantly higher than CK (P<0.01).During cuttage 30d, 100mgL
-1boron+200mgL
-1the lower cuttings ABA content of IBA process is significantly higher than all process (P<0.01), is secondly 2% sucrose+200mgL
-1iBA, 5% sucrose+200mgL
-1iBA and CK process, 300mgL
-1boron+200mgL
-1iBA and 200mgL
-1boron+200mgL
-1cuttings hormone-content under IBA process is significantly lower than all process (P<0.01).As cuttage 45d, 50mgL
-1vB+200mgL
-1iBA, 100mgL
-1vB+200mgL
-1the cuttings ABA content of IBA and CK process is significantly higher than all the other respectively process (P<0.01), with the addition of cuttings under the IBA root-growing agent process of boron and sucrose generally when cuttage 45d ABA content comparatively with the addition of Cobastab and independent IBA process is on the low side.Illustrate that the interpolation of boron promotes southern high clump blueberry to take root in a large number at Adventitious root initiation period (30 ~ 45d) relevant with reduction cuttings cortex ABA content.
The different root-growing agent of table 11 is on the impact of Herbert endogenous aba content
As shown in Table 12, during cuttage 1d, the lower cuttings hormone IAA/ABA ratio of CK process is significantly higher than all the other respectively process (P<0.01).As cuttage 15d, all IBA root-growing agent and 200mgL that with the addition of Cobastab
-1boron+200mgL
-1iBA, 300mgL
-1boron+200mgL
-1its hormone ratio of cuttings under IBA process is higher than CK.Cuttage 30d, 200mgL
-1boron+200mgL
-1the lower hormone ratio of IBA process is significantly higher than all the other respectively process (P<0.01), is secondly 50mgL
-1vB+200mgL
-1iBA and 300mgL
-1boron+200mgL
-1iBA process.Cuttage 45d, the cuttings IAA/ABA ratio under all IBA root-growing agent process that with the addition of boron is significantly higher than all the other respectively process (P<0.01), and in all process, hormone ratio from high to low successively: 300mgL
-1boron > 100mgL
-1boron > 200mgL
-1boron > 200mgL
-1vB > 100mgL
-1vB > 2% sucrose > 50mgL
-1vB > 5% sucrose > CK > 10% sucrose.Result shows, with the addition of the IBA root-growing agent process of boron, significantly improves Herbert Adventitious root initiation period (30 ~ 45d) cuttings cortex IAA content, reduces ABA content simultaneously, substantially increase IAA/ABA ratio thus be beneficial to and take root.
The different root-growing agent of table 12 is on the impact of Herbert Endogenous IAA/ABA
Under the process of embodiment 5 kind blue Feng Butong root-growing agent, Difference of Endogenous Hormones is analyzed
Blue its endogenous IAA of rich cuttings of different root-growing agent process, ABA, IAA/ABA difference analysis are as shown in table 13, table 14, table 15.As shown in Table 13, during cuttage 1d, its IAA content of cuttings of all IBA root-growing agent process through with the addition of boron generally respectively processes higher than all the other, wherein 300mgL
-1boron+50mgL
-1the lower hormone-content of IBA process is the highest; As cuttage 15d, the cuttings IAA content that with the addition of after the IBA root-growing agent process of boron, Cobastab is significantly higher than and with the addition of sucrose and IBA process (P<0.01) separately, and the lower cuttings IAA content of CK process is minimum in all process; As cuttage 30d, 200mgL
-1boron+50mgL
-1the cuttings IAA content of IBA process is significantly higher than all the other respectively process (P<0.01), is secondly 100mgL
-1boron+50mgL
-1iBA and 10% sucrose+50mgL
-1the process of IBA, its IAA content of cuttings under all IBA root-growing agent process that with the addition of sucrose, boron, Cobastab is all higher than CK; As cuttage 45d, the process from high to low of IAA content successively: 300mgL
-1boron > 200mgL
-1boron > 2% sucrose > 200mgL
-1vB > 100mgL
-1boron > 100mgL
-1vB > 50mgL
-1vB > 5% sucrose > 10% sucrose > CK.In conjunction with the dynamic concept cls analysis result to the blue rich rooting percent of kind, with the addition of 300mgL
-1the IBA root-growing agent of boron can improve blue rich in Adventitious root initiation period (30 ~ 45d) cuttings Adventitious root initiation rate fast, and to improve cuttings cortex IAA content closely related with it.
The different root-growing agent of table 13 is on the impact of the rich Endogenous IAA content of indigo plant
As shown in Table 14, cuttage 1d, all its ABA content of cuttings after the IBA root-growing agent process that with the addition of boron is all significantly higher than CK, and the cuttings ABA content that with the addition of the IBA root-growing agent process of sucrose is significantly lower than CK (P<0.01); As cuttage 15d, the cuttings ABA content that with the addition of boron process is still significantly higher than all the other respectively process (P<0.01); As cuttage 30d, 5% sucrose+50mgL
-1iBA and 100mgL
-1vB+50mgL
-1cuttings ABA content under IBA process is significantly higher than all the other respectively process (P<0.01), and the cuttings ABA content that with the addition of boron process is remarkable in CK (P<0.01); As cuttage 45d, the process from high to low of ABA content successively: 50mgL
-1vB > 200mgL
-1vB > CK > 100mgL
-1boron > 100mgL
-1vB > 5% sucrose > 300mgL
-1boron > 2% sucrose > 200mgL
-1boron > 10% sucrose.By the investigation of situation of finally taking root to the rich cuttings of indigo plant, find the cuttage rooting DeGrain that with the addition of the IBA root-growing agent process of Cobastab, it is relevant at the ABA content of Adventitious root initiation period (30 ~ 45d) that this and Cobastab improve blue rich cuttings cortex.
The different root-growing agent of table 14 is on the impact of the rich endogenous aba content of indigo plant
As shown in Table 15, cuttage 1d, 200mgL
-1vB+50mgL
-1the cuttings IAA/ABA of IBA process is significantly higher than all the other respectively process (P<0.01), is secondly 10% sucrose+50mgL
-1iBA and 5% sucrose+50mgL
-1the process of IBA; As cuttage 15d, the cuttings IAA/ABA that with the addition of the IBA root-growing agent process of Cobastab is generally significantly higher than all the other respectively process (P<0.01), except 300mgL
-1boron+50mgL
-1beyond IBA process, the cuttings IAA/ABA of CK process is minimum; As cuttage 30d, through 200mgL
-1boron+50mgL
-1the cuttings IAA/ABA of IBA process is significantly higher than all the other respectively process (P<0.01), is secondly 50mgL
-1vB+50mgL
-1iBA and 100mgL
-1boron+50mgL
-1the process of IBA, and all with the addition of sucrose, boron, Cobastab its cuttings IAA/ABA of process be all significantly higher than CK (P<0.01); When cuttage is to 45d, IAA/ABA process from high to low successively: 10% sucrose > 300mgL
-1boron > 200mgL
-1boron > 2% sucrose > 100mgL
-1boron > 5% sucrose > 200mgL
-1vB > 100mgL
-1vB > 50mgL
-1vB > CK.Due to 300mgL
-1its ABA content of the cuttings of boron compound IBA process is significantly higher than 10% sucrose compound IBA process, causes its cuttings IAA/ABA not as good as 10% saccharose treatment.By to the rich cuttage rooting dynamic monitoring of indigo plant, find when cuttage 45d, through 300mgL
-1the rooting percent of boron compound IBA process is the highest, and all its rooting rates of cuttings that with the addition of boron process are all higher than the process adding sucrose.Illustrating that the IBA root-growing agent that with the addition of boron significantly improves blue rich cuttings cortex IAA content is promote its main cause of taking root.
The different root-growing agent of table 15 is on the impact of the rich Endogenous IAA/ABA of indigo plant
Claims (4)
1. a blueberry green wood cutting seedling-cultivating method, is characterized in that comprising the following steps:
1) selection of cuttings: in 3 ~ April, choose that 1 year stiff degree is large, maturity good and the position, vegetative shoot middle and lower part of health as cuttings;
2) process of cuttings: branch is pruned growth 7 ~ 10cm, is with the cuttings of 4 ~ 5 axillalry buds and top 1 ~ 2 leaf, and upper clip is truncate apart from bud 1cm, lower clip 45 ° of bevels;
3) immersion of cuttings: soak 8 ~ 10h with the root-growing agent of boron+IBA;
4) cuttage: matrix is watered permeable guarantee humidity before cuttage but not ponding, cuttage, by after cutting sterilization ling more than 1000 times immersion base portion 10s of handling well, is vertically inserted in matrix by cuttage same day;
5) water management: the humidity of matrix maintains more than 80%, temperature controls at 20 ~ 28 DEG C;
6) management after cuttage: apply fertilizer gradually after cuttage rooting, applying fertilizer applies with the form of liquid fertilizer, and concentration is no more than 3%, carries out 1 fertilising weekly to the green branch of blueberry cuttage.
2. a kind of blueberry green wood cutting seedling-cultivating method according to claim 1, is characterized in that: the aseptic water as solvent of root-growing agent described in step (3), preparation root-growing agent, and ensure that IBA concentration is 50mg/L, the concentration of boron is 300mg/L.
3. a kind of blueberry green wood cutting seedling-cultivating method according to claim 1, is characterized in that: described matrix is for utilizing liver moss soil as cutting medium, and adjust ph is 5.5, and the previous day is carried out sprinkling process to slotting machine matrix 1000 times of carbendazim in cuttage.
4. a kind of blueberry green wood cutting seedling-cultivating method according to claim 1, is characterized in that: described cuttage is specially the degree of depth inserting matrix and accounts for 2/3 of whole cutting, and spacing is 5cm × 10cm.
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| CN107318647A (en) * | 2017-06-06 | 2017-11-07 | 山东大丰园农业有限公司 | A kind of root-growing agent and its outside sprout-cultivating-bottle method for blueberry tissue culture outside sprout-cultivating-bottle radication |
| CN107396726A (en) * | 2017-07-19 | 2017-11-28 | 福建师范大学 | A kind of method for culturing seedlings for improving Common zenia cuttage root-taking quantity |
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| CN115104441A (en) * | 2022-08-12 | 2022-09-27 | 河南科技大学 | Efficient rooting method for blueberry twig cuttage |
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| CN114794121A (en) * | 2022-05-24 | 2022-07-29 | 浙江师范大学 | Treatment agent, stock solution and cutting method for improving rooting and survival rate of cut blueberry branches |
| CN115104441A (en) * | 2022-08-12 | 2022-09-27 | 河南科技大学 | Efficient rooting method for blueberry twig cuttage |
| CN115104441B (en) * | 2022-08-12 | 2023-06-30 | 河南科技大学 | Efficient rooting method for blueberry twig cuttage |
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