CN104521616A - Alfalfa non-separation quick rooting and cutting device and application method thereof - Google Patents
Alfalfa non-separation quick rooting and cutting device and application method thereof Download PDFInfo
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- CN104521616A CN104521616A CN201510004821.7A CN201510004821A CN104521616A CN 104521616 A CN104521616 A CN 104521616A CN 201510004821 A CN201510004821 A CN 201510004821A CN 104521616 A CN104521616 A CN 104521616A
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- cup
- cuttage
- alfalfa
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
Abstract
The invention discloses an alfalfa non-separation quick rooting and cutting device which comprises a cutting cup and a fixing device (3), the cutting cup comprises a cup body (1) and a cup cover (2), the cup body (1) is connected with the cup cover (2) in a threaded mode, and the center of the cup cover (2) and the bottom of the cup body (1) are each provided with a small hole with a diameter of one centimeter; the cutting cup is fixed on the fixing device (3), and the cutting cup is used for containing culture substrates. The alfalfa non-separation quick rooting and cutting device and an application method thereof further disclose an alfalfa non-separation quickly rooting cutting method, and the alfalfa non-separation quickly rooting cutting device and the application method thereof have the advantages of being high in efficiency and survival rate.
Description
Technical field
The present invention relates to a kind of sticking device and using method thereof, be specifically related to a kind of alfalfa Non in vitro Rapid Rooting sticking device and using method thereof.
Background technology
Alfalfa (Medicago sativa) belongs to pulse family perennial plant for clover, has the good reputation of King of Pasture, and whole world Alfalfa Growing area, more than ten million hectare, also has long cultivation history in China.Because its crude protein content is high, good palatability, be that the best of ruminant is fed one of herbage, alfalfa also can be used as vegetables human consumption and is used as medicine as traditional Chinese medicine.
Alfalfa has the advantages such as cold-resistant, drought-enduring, Salt And Alkali Tolerance, barren-resistant, strong adaptability, is widely cultivated in northern China particularly northwestward drought area, in improving the ecological environment, transforming agricultural production, has played important function.Given this, cultivate high yield, degeneration-resistant, high-quality alfalfa new varieties just seem very urgent, and utilize male sterile to obtain heterosis breeding and natural selection breeding is conventional alfalfa breeding method.Alfalfa is cross-pollinatd plant, there is Self-Incompatibility and self progeny's slump of disastrous proportions phenomenon.If therefore need preservation alfalfa male sterile plants or merit plant for further breeding and research, usually need to carry out asexual cottage propagation to specific plant.At present, clip comprises the branch of 1 ~ 2 joint and cuts off its side shoot and insert soil to carry out detached insertion be common alfalfa cottage method, conventional alfalfa cottage method is comparatively large by the impact of season, vegetative period, temperature etc., there is the shortcomings such as survival rate of taking root can not be guaranteed and cuttage primary growth is slow.
Summary of the invention
The invention provides a kind of alfalfa Non in vitro Rapid Rooting sticking device, apparatus of the present invention can complete the cuttage of alfalfa Non in vitro Rapid Rooting.
Present invention also offers a kind of alfalfa Non in vitro Rapid Rooting cottage method.
A kind of alfalfa Non in vitro Rapid Rooting sticking device, wherein, described alfalfa Non in vitro Rapid Rooting sticking device comprises cuttage cup and fixture, described cuttage cup comprises cup and bowl cover, described cup is threaded with described bowl cover, and described bowl cover center and described bottom of cup have diameter to be the aperture of 1cm; Described cuttage cup is fixed in the middle part of described fixture, and described cuttage cup is used for holding culture matrix.
Alfalfa Non in vitro Rapid Rooting sticking device of the present invention, wherein, described fixture is the straight-bar with retainer ring, and be placed on bottom described cuttage cup on the retainer ring of described fixture, the straight-bar of top and described fixture bands together; Culture basin or large Tanaka is vertically inserted in bottom the straight-bar of described fixture;
The long 40cm of described straight-bar, diameter 0.8cm, lower end is taper, and be welded with a becket apart from 10cm place, upper end, diameter is 5cm, described becket has the wide breach of 2cm.
Alfalfa Non in vitro Rapid Rooting sticking device of the present invention, it is characterized in that, described fixture comprises 3 straight-bars and 1 plummer net, and described 3 straight-bars are vertically inserted in Culture basin or large Tanaka, and the line of the floor projection of described 3 straight-bars is triangle; Described plummer net is fixed on described 3 straight-bars, and described plummer net is horizontal positioned; It is online that described cuttage cup is placed on described plummer, described cuttage cup quantity >=1.
Alfalfa Non in vitro Rapid Rooting sticking device of the present invention, wherein, described fixture is metal material, and described 3 straight-bar diameters are 1cm, and length is 50cm; Described plummer net is the square of length of side 48cm, and described plummer net transverse grid spacing 3cm, longitudinal grid spacing is 16cm.
Alfalfa Non in vitro Rapid Rooting sticking device of the present invention, wherein, the fixed form that described plummer net is fixed on described 3 straight-bars is as follows: described fixture comprises removable fixed card, described removable fixed card is fixed with internal diameter 1cm, the sleeve pipe of external diameter 1.2cm, length 5cm, for being enclosed within described straight-bar, a hold-down nut is had, for described removable fixed card is fixed on described straight-bar in the middle part of described sleeve pipe; The long 5cm of described removable fixed card, wide 3.5cm, thickness 1mm, described removable fixed card is provided with diameter 5mm aperture, for described plummer net and described 3 straight-bars being bundled through static line.
A kind of alfalfa Non in vitro Rapid Rooting cottage method, is characterized in that, comprise the steps:
A, select robust growth in large Tanaka or Culture basin and one or more tenderer alfalfa branch, cut off its main side shoot, retain top axillalry bud and shorter side shoot, then by described robust growth and tenderer alfalfa branch first extend upwardly into from the small hole at bottom part of described cuttage cup again through after the retainer ring of described fixture or plummer net, branch upper end is made to exceed bowl cover more than the upper surface 5cm of described cuttage cup, prune and be positioned at the part epidermis of cuttage cup alfalfa branch, forming width is 1 ~ 2cm ring-type wound, culture matrix is loaded described cuttage cup, space is left between described culture matrix upper surface and described cuttage cup rim of a cup, described alfalfa branch is near described cuttage cup axis, root-growing agent is poured in described cuttage cup, till cup mesostroma is all soaked, top branch higher than cup edge is tightened described bowl cover and described cup through after the aperture on described bowl cover, described fixture is fixed in large Tanaka or Culture basin, described cuttage is had the described cuttage cup of described alfalfa branch to be fixed in described fixture,
Branch is cut off with parent from cuttage cup bottom after 1 ~ 2 week and is separated by B, induction alfalfa branch culture of rootage, the alfalfa branch of taking root in cup and matrix of taking root thereof take out together and move into Culture basin or large Tanaka by continuation cultivation for 2 ~ 3 weeks after alfalfa branch grows a large amount of root from cuttage cup.
Alfalfa Non in vitro Rapid Rooting cottage method of the present invention, wherein, the culture of rootage condition in described step B is: within every 3 ~ 5 days, add suitable quantity of water with syringe from bowl cover mesopore, ensures that matrix of taking root is in moisture state for a long time.
Alfalfa Non in vitro Rapid Rooting cottage method of the present invention, wherein, described culture matrix is that the mixture of land for growing field crops soil, Nutrition Soil, perlite and vermiculite obtains according to the ratio mixing that volume ratio is 2:2:1:1; Using containing concentration when watering first after loading cuttage cup is the pouring of 5 ~ 10mg/L indolebutyric acid aqueous solution;
The collocation method of the described heteroauxin aqueous solution is: first with 75% alcohol for solvent preparation indolebutyric acid mother liquor, described in described indolebutyric acid mother liquor, indolebutyric acid concentration is 5 ~ 10mg/mL, then with distilled water, described indolebutyric acid mother liquor is diluted 1000 ~ 2000 times.
Alfalfa Non in vitro Rapid Rooting cottage method of the present invention, wherein, the described culture matrix upper surface in described cuttage cup is apart from described cuttage cup upper surface 1cm.
Alfalfa Non in vitro Rapid Rooting cottage method of the present invention, wherein, inserts 1 ~ 5 alfalfa branch and carries out cuttage root-taking cultivation in each described cuttage cup.
Alfalfa Non in vitro Rapid Rooting sticking device of the present invention and using method difference from prior art thereof are:
Alfalfa Non in vitro Rapid Rooting cottage method of the present invention is by have selected suitable alfalfa branch and the cultivation of Non in vitro cuttage root-taking is carried out at suitable position, survival rate is high, the suitableeest cuttage root-taking culture matrix and best growth-promoting root agent concentration are prepared, first time, by making the specific cuttage root-taking culture apparatus being applicable to the cuttage of alfalfa Non in vitro, establishes a kind of rapidly and efficiently Non in vitro cuttage root-taking cultivating system of alfalfa.
Tool has the following advantages:
The vigorous alfalfa branch of the inventive method growth selection inserts special cuttage and cultivates in cup, be fixed in special carrying fixture and carry out the cultivation of Non in vitro cuttage root-taking, compared with the in vitro brachyplast cottage method of routine, branch is not separated with parent by the inventive method cuttage initial stage, thus greatly reduces the lethality at cuttage initial stage; Owing to only having cut off the larger side shoot of near sites of taking root, remain more little side shoot, lateral bud and terminal bud above position of taking root, while taking root, these side shoots, lateral bud and terminal bud can grow fully, the seedling that the cuttage seeding of final acquisition obtains than normal cutting propagation method is more healthy and stronger, is beneficial to transplant survival.During cuttage by cuttage cup at the bottom of the cup branch epidermis to prune a part, form long 1 ~ 2cm wound and be convenient to Rapid Rooting.
The cuttage culture matrix selected and root-growing agent have good adaptability to taking root of alfalfa cuttage branch, rooting rate reaches more than 98%, compared with the in vitro brachyplast cottage method of routine, the method can make alfalfa branch rootage duration foreshorten to 1-2 week, cuttage branch root length after 4 weeks is cultivated in cuttage can reach more than 15cm, newborn branch height reaches more than 20cm, and root growth speed is 2-4 times of common cuttage root-taking method;
The alfalfa cuttage seedlings root that the inventive method obtains physically well develops, particularly main root is sturdy, side root is flourishing, seedling is healthy and strong, transplanting survival rate reaches more than 96%, and the inventive method solves the problems such as the conventional in vitro brachyplast cottage method growth of cuttage seedlings of conventional alfalfa is slow, root system is short and small, side root rare, survival rate is low; The inventive method is all applicable at each growth period for the alfalfa of more than height 15cm, all applicable during non-sleep from spring to autumn, and the conventional in vitro brachyplast cottage method of conventional alfalfa is generally suitable for initial bloom stage and squaring period, when temperature is higher, be not suitable for cuttage.Therefore, the adaptability of this inventive method to environment and vegetative period is stronger compared with normal cutting propagation method;
The inventive method is simple to operate, and simply pruned by applicable alfalfa branch in rear direct insertion cuttage cultivation cup and carry out culture of rootage, a cuttage cup can insert many branches and carry out cuttage cultivation simultaneously.Culture matrix in cup can well seal by cuttage bowl cover, prevents the moisture in matrix from evaporating, and keeps matrix moistening, and then is beneficial to and takes root.The features such as it is short that this cottage method has rootage duration, and quality of rooting is good, have increased substantially the asexual cottage propagation speed of alfalfa and quality, for large-scale alfalfa cuttage breeding provides guarantee.
The special Non in vitro cuttage culture apparatus of the inventive method is applicable to the cuttage of greenhouse and field alfalfa material simultaneously, in addition for adapting to the cuttage of a small amount of branch and the cuttage of a large amount of branch has made different cuttage fixtures.
The present invention well solve the survival rate of traditional alfalfa cottage method low, take root slowly, root system is short and small, the cuttage problem such as upper part poor growth incipiently, have selected suitable cuttage culture matrix and best root-growing agent concentration, achieve the Non in vitro cuttage of alfalfa branch, considerably increase survival rate and rooting rate, the cuttage alfalfa shoot root system obtained physically well develops, main root is sturdy, and side root is flourishing, establishes a kind of rapidly and efficiently culture of rootage system of alfalfa cuttage.The inventive method has feature simple to operate, rapidly and efficiently and greatly to improve alfalfa cuttage seeding quality of rooting, and then has increased substantially alfalfa cuttage seeding transplanting survival rate.
Have employed special cuttage culture apparatus in the present invention, comprise cuttage cup and fixing bogey.Have aperture in the middle of bottom of cup and bowl cover, play and allow alfalfa cuttage branch from cup through the effect of cup.Culture matrix and cuttage branch bottom are provided approximate totally-enclosed environment by cuttage cup, ensure that branch is for a long time in moistening environment, is convenient to culture of rootage.Fixing bogey is divided into many cuttages cup fixture and single cuttage cup fixture, the latter more succinctly facilitates than the former assembling, the becket of single cuttage fixture is provided with breach, during cuttage can first will the clover branch of cuttage through the aperture of bottom of cup and the aperture of bowl cover, then the clover branch below cuttage cup enters retainer ring by breach, and clover following bottom cuttage cup when facilitating cuttage like this enters retainer ring; Single cuttage cup fixture can be selected when carrying out the cuttage of a small amount of alfalfa branch, many cuttages cup fixture when needing to carry out a large amount of branch cutting, can be selected.
Below in conjunction with accompanying drawing, alfalfa Non in vitro Rapid Rooting sticking device of the present invention and using method thereof are described further.
Accompanying drawing explanation
Fig. 1 is the fixture schematic diagram that in alfalfa Non in vitro Rapid Rooting sticking device of the present invention, glass sticking device inserted by a kind of list;
Fig. 2 is that in alfalfa Non in vitro Rapid Rooting cottage method of the present invention, a kind of large Tanaka singly inserts cup cottage method effect schematic diagram;
Fig. 3 is that in alfalfa Non in vitro Rapid Rooting cottage method of the present invention, cup cottage method effect schematic diagram inserted by a kind of potted plant middle list;
Fig. 4 is a kind of fixture schematic diagram inserting cup sticking device in alfalfa Non in vitro Rapid Rooting sticking device of the present invention more;
Fig. 5 is a kind of large Tanaka's how slotting cup cottage method effect schematic diagram in alfalfa Non in vitro Rapid Rooting cottage method of the present invention;
Fig. 6 be in alfalfa Non in vitro Rapid Rooting cottage method of the present invention a kind of potted plant in insert cup cottage method effect schematic diagram more.
Embodiment
Embodiment 1
A kind of alfalfa Non in vitro Rapid Rooting sticking device, wherein, described alfalfa Non in vitro Rapid Rooting sticking device comprises cuttage cup and fixture 3, described cuttage cup comprises cup 1 and bowl cover 2, described cup 1 is threaded with described bowl cover 2, has diameter to be the aperture of 1cm bottom described bowl cover 2 center and described cup 1; Described cuttage cup is fixed in the middle part of described fixture 3, and described cuttage cup is used for holding culture matrix.
Above scheme may be used for the cuttage of alfalfa Non in vitro Rapid Rooting cuttage, provides preferred version on this basis:
A kind of alfalfa Non in vitro Rapid Rooting sticking device, fixture 3 is the straight-bar with retainer ring, and be placed on bottom described cuttage cup on the retainer ring of described fixture 3, the straight-bar of top and described fixture 3 bands together; Culture basin or large Tanaka is vertically inserted in bottom the straight-bar of described fixture 3;
The long 40cm of described straight-bar, diameter 0.8cm, lower end is taper, and be welded with a becket apart from 10cm place, upper end, diameter is 5cm, described becket has the wide breach of 2cm, and wherein, fixture 3 as shown in Figure 1.
Another kind of preferred version is:
A kind of alfalfa Non in vitro Rapid Rooting sticking device, fixture 3 comprises 3 straight-bars and 1 plummer net 4, and described 3 straight-bars are vertically inserted in Culture basin or large Tanaka, and the line of the floor projection of described 3 straight-bars is triangle; Described plummer net 4 is fixed on described 3 straight-bars, and described plummer net 4 is horizontal positioned; Described cuttage cup is placed on described plummer net 4, described cuttage cup quantity >=1.
Described fixture 3 is metal material, and described 3 straight-bar diameters are 1cm, and length is 50cm; The long square for length of side 48cm of described plummer net, described plummer net transverse grid spacing 3cm, longitudinal grid spacing is 16cm.
The fixed form that described plummer net 4 is fixed on described 3 straight-bars is as follows: described fixture 3 comprises removable fixed card, described removable fixed card is fixed with internal diameter 1cm, external diameter 1.2cm, the sleeve pipe of length 5cm, for being enclosed within described straight-bar, a hold-down nut is had, for described removable fixed card is fixed on described straight-bar in the middle part of described sleeve pipe; The long 5cm of described removable fixed card, wide 3.5cm, thickness 1mm, described removable fixed card is provided with diameter 5mm aperture, and for being bundled with described 3 straight-bars by described plummer net 4 through static line, wherein, fixture 3 as shown in Figure 4.
Embodiment 2
Adopt an alfalfa Non in vitro Rapid Rooting cottage method for sticking device described in embodiment 1, comprise the steps:
A, select robust growth in large Tanaka or Culture basin and one or more tenderer alfalfa branch, cut off its main side shoot, retain top axillalry bud and shorter side shoot, then by described robust growth and tenderer alfalfa branch first extend upwardly into from the small hole at bottom part of described cuttage cup again through after the retainer ring of described fixture 3 or plummer net, branch upper end is made to exceed bowl cover more than the upper surface 5cm of described cuttage cup, prune and be positioned at the part epidermis of cuttage cup alfalfa branch, forming width is 1 ~ 2cm ring-type wound, culture matrix is loaded described cuttage cup, space is left between described culture matrix upper surface and described cuttage cup rim of a cup, described alfalfa branch is near described cuttage cup axis, root-growing agent is poured in described cuttage cup, till cup mesostroma is all soaked, top branch higher than cup edge is tightened described bowl cover 2 and described cup 1 through after the aperture on described bowl cover 2, described fixture 3 is fixed in large Tanaka or Culture basin, the described cuttage cup of described alfalfa branch described cuttage is had to be fixed in described fixture 3, wherein, large Tanaka singly inserts cup cottage method effect schematic diagram as shown in Figure 2, and potted plant middle list inserts cup cottage method effect schematic diagram as shown in Figure 3, as shown in Figure 5, in potted plant, many slotting cup cottage method effect schematic diagrames are as shown in Figure 6 for large Tanaka how slotting cup cottage method effect schematic diagram,
Branch is cut off with parent from cuttage cup bottom after 1 ~ 2 week and is separated by B, induction alfalfa branch culture of rootage, the alfalfa branch of taking root in cup and matrix of taking root thereof take out together and move into Culture basin or large Tanaka by continuation cultivation for 2 ~ 3 weeks after alfalfa branch grows a large amount of root from cuttage cup.
Above scheme can complete the cuttage of alfalfa Non in vitro Rapid Rooting, provides preferred version on this basis:
Culture of rootage condition in step B is: within every 3 ~ 5 days, add suitable quantity of water with syringe from bowl cover mesopore, ensures that matrix of taking root is in moisture state for a long time; Culture matrix is that the mixture of land for growing field crops soil, Nutrition Soil, perlite and vermiculite obtains according to the ratio mixing that volume ratio is 2:2:1:1; Using containing concentration when watering first after loading cuttage cup is the pouring of 5 ~ 10mg/L indolebutyric acid aqueous solution;
The collocation method of the described heteroauxin aqueous solution is: first with 75% alcohol for solvent preparation indolebutyric acid mother liquor, described in described indolebutyric acid mother liquor, indolebutyric acid concentration is 5 ~ 10mg/mL, then with distilled water, described indolebutyric acid mother liquor is diluted 1000 ~ 2000 times; Described culture matrix upper surface in cuttage cup is apart from described cuttage cup upper surface 1cm; Insert 1 ~ 5 alfalfa branch in each described cuttage cup and carry out cuttage root-taking cultivation.The present embodiment method is for alfalfa " No. 1, middle lucerne " cuttage root-taking, and the data of the in vitro brachyplast cottage method of routine compare in table 1.
Table 1 " No. 1, middle lucerne " cuttage root-taking cultivates the effectiveness comparison of 4 weeks latter two rooting methods
Above-described embodiment is only be described the preferred embodiment of the present invention; not scope of the present invention is limited; under not departing from the present invention and designing the prerequisite of spirit; the various distortion that those of ordinary skill in the art make technical scheme of the present invention and improvement, all should fall in protection domain that claims of the present invention determines.
Claims (10)
1. an alfalfa Non in vitro Rapid Rooting sticking device, it is characterized in that, described alfalfa Non in vitro Rapid Rooting sticking device comprises cuttage cup and fixture (3), described cuttage cup comprises cup (1) and bowl cover (2), described cup (1) is threaded with described bowl cover (2), and described bowl cover (2) center and described cup (1) bottom all have diameter to be the aperture of 1cm; Described cuttage cup is fixed on described fixture (3) middle part, and described cuttage cup is used for holding culture matrix.
2. alfalfa Non in vitro Rapid Rooting sticking device according to claim 1, it is characterized in that, described fixture (3) is the straight-bar with retainer ring, be placed on bottom described cuttage cup on the retainer ring of described fixture (3), the straight-bar of top and described fixture (3) bands together; Culture basin or large Tanaka is vertically inserted in bottom the straight-bar of described fixture (3);
The long 40cm of described straight-bar, diameter 0.8cm, lower end is taper, and be welded with a becket apart from 10cm place, upper end, described becket diameter is 5cm, described becket has the wide breach of 2cm.
3. alfalfa Non in vitro Rapid Rooting sticking device according to claim 1, it is characterized in that, described fixture (3) comprises 3 straight-bars and 1 plummer net (4), described 3 straight-bars are vertically inserted in Culture basin or large Tanaka, and the line of the floor projection of described 3 straight-bars is triangle; Described plummer net (4) is fixed on described 3 straight-bars, and described plummer net (4) is horizontal positioned; Described cuttage cup is placed on described plummer net (4), described cuttage cup quantity >=1.
4. alfalfa Non in vitro Rapid Rooting sticking device according to claim 3, it is characterized in that, described fixture (3) is metal material, and described 3 straight-bar diameters are 1cm, and length is 50cm; Described plummer net is the square of length of side 48cm, and described plummer net transverse grid spacing 3cm, longitudinal grid spacing is 16cm.
5. alfalfa Non in vitro Rapid Rooting sticking device according to claim 4, it is characterized in that, the fixed form that described plummer net (4) is fixed on described 3 straight-bars is as follows: described fixture (3) comprises removable fixed card, described removable fixed card is fixed with internal diameter 1cm, external diameter 1.2cm, the sleeve pipe of length 5cm, for being enclosed within described straight-bar, a hold-down nut is had, for described removable fixed card is fixed on described straight-bar in the middle part of described sleeve pipe; The long 5cm of described removable fixed card, wide 3.5cm, thickness 1mm, described removable fixed card is provided with diameter 5mm aperture, for described plummer net (4) and described 3 straight-bars being bundled through static line.
6. use an alfalfa Non in vitro Rapid Rooting cottage method for claim 1 ~ 5 alfalfa Non in vitro Rapid Rooting sticking device described in any one, it is characterized in that, comprise the steps:
A, select robust growth in large Tanaka or Culture basin and one or more tenderer alfalfa branch, cut off its main side shoot, retain top axillalry bud and shorter side shoot, then by described robust growth and tenderer alfalfa branch first extend upwardly into from the small hole at bottom part of described cuttage cup again through after the retainer ring of described fixture (3) or plummer net, branch upper end is made to exceed bowl cover more than the upper surface 5cm of described cuttage cup, prune and be positioned at the part epidermis of cuttage cup alfalfa branch, forming width is 1 ~ 2cm ring-type wound, culture matrix is loaded described cuttage cup, space is left between described culture matrix upper surface and described cuttage cup rim of a cup, described alfalfa branch is near described cuttage cup axis, root-growing agent is poured in described cuttage cup, till cup mesostroma is all soaked, top branch higher than cup edge is tightened described bowl cover (2) and described cup (1) through after the aperture on described bowl cover (2), described fixture (3) is fixed in large Tanaka or Culture basin, described cuttage is had the described cuttage cup of described alfalfa branch to be fixed in described fixture (3),
Branch is cut off with parent from cuttage cup bottom after 1 ~ 2 week and is separated by B, induction alfalfa branch culture of rootage, the alfalfa branch of taking root in cup and matrix of taking root thereof take out together and move into Culture basin or large Tanaka by continuation cultivation for 2 ~ 3 weeks after alfalfa branch grows a large amount of root from cuttage cup.
7. alfalfa Non in vitro Rapid Rooting cottage method according to claim 6, it is characterized in that, the culture of rootage condition in described step B is: within every 3 ~ 5 days, add suitable quantity of water with syringe from bowl cover mesopore, and the matrix that ensures to take root is in moisture state for a long time.
8. alfalfa Non in vitro Rapid Rooting cottage method according to claim 7, is characterized in that, described culture matrix is that the mixture of land for growing field crops soil, Nutrition Soil, perlite and vermiculite obtains according to the ratio mixing that volume ratio is 2:2:1:1; Using containing concentration when watering first after loading cuttage cup is the pouring of 5 ~ 10mg/L indolebutyric acid aqueous solution.
The collocation method of the described heteroauxin aqueous solution is: first with 75% alcohol for solvent preparation indolebutyric acid mother liquor, described in described indolebutyric acid mother liquor, indolebutyric acid concentration is 5 ~ 10mg/mL, then with distilled water, described indolebutyric acid mother liquor is diluted 1000 ~ 2000 times.
9. alfalfa Non in vitro Rapid Rooting cottage method according to claim 8, it is characterized in that, the described culture matrix upper surface in described cuttage cup is apart from described cuttage cup upper surface 1cm.
10. alfalfa Non in vitro Rapid Rooting cottage method according to claim 8, is characterized in that, inserts 1 ~ 5 alfalfa branch and carry out cuttage root-taking cultivation in each described cuttage cup.
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| CN201510004821.7A CN104521616B (en) | 2015-01-06 | 2015-01-06 | A kind of alfalfa Non in vitro Rapid Rooting sticking device and using method thereof |
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| CN108605537A (en) * | 2018-05-08 | 2018-10-02 | 李明 | A kind of method of purplefruit holly leaf epicormic branch cutting |
| CN110651670A (en) * | 2018-06-29 | 2020-01-07 | 中国农业科学院北京畜牧兽医研究所 | Alfalfa cuttage method |
| CN111133903A (en) * | 2020-01-16 | 2020-05-12 | 青岛农业大学 | Alfalfa water culture cutting propagation method |
| CN112166846A (en) * | 2020-09-23 | 2021-01-05 | 青岛农业大学 | Water planting cutting propagation method for alfalfa in cut shape |
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| GB2377152A (en) * | 2001-07-06 | 2003-01-08 | Seymour Cemoun Daniels | Device for taking cuttings from plants |
| CN1422517A (en) * | 2002-12-25 | 2003-06-11 | 重庆海浪科技实业（集团）有限公司 | Vegetative breeding cultivation method for alfalfa |
| CN204443315U (en) * | 2015-01-06 | 2015-07-08 | 中国农业科学院北京畜牧兽医研究所 | A kind of alfalfa Non in vitro Rapid Rooting sticking device |
-
2015
- 2015-01-06 CN CN201510004821.7A patent/CN104521616B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2377152A (en) * | 2001-07-06 | 2003-01-08 | Seymour Cemoun Daniels | Device for taking cuttings from plants |
| CN2503703Y (en) * | 2001-09-12 | 2002-08-07 | 程晓辉 | Cuttage vessel for plant cultivation |
| CN2529503Y (en) * | 2002-02-26 | 2003-01-08 | 张平 | Nursery stock cuttage/cultivation appts. |
| CN1422517A (en) * | 2002-12-25 | 2003-06-11 | 重庆海浪科技实业（集团）有限公司 | Vegetative breeding cultivation method for alfalfa |
| CN204443315U (en) * | 2015-01-06 | 2015-07-08 | 中国农业科学院北京畜牧兽医研究所 | A kind of alfalfa Non in vitro Rapid Rooting sticking device |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108605537A (en) * | 2018-05-08 | 2018-10-02 | 李明 | A kind of method of purplefruit holly leaf epicormic branch cutting |
| CN110651670A (en) * | 2018-06-29 | 2020-01-07 | 中国农业科学院北京畜牧兽医研究所 | Alfalfa cuttage method |
| CN111133903A (en) * | 2020-01-16 | 2020-05-12 | 青岛农业大学 | Alfalfa water culture cutting propagation method |
| CN112166846A (en) * | 2020-09-23 | 2021-01-05 | 青岛农业大学 | Water planting cutting propagation method for alfalfa in cut shape |
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