CN109247238A - A kind of five leaflet maple tissue culture outside sprout-cultivating-bottle radication methods - Google Patents
A kind of five leaflet maple tissue culture outside sprout-cultivating-bottle radication methods Download PDFInfo
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- CN109247238A CN109247238A CN201811449180.6A CN201811449180A CN109247238A CN 109247238 A CN109247238 A CN 109247238A CN 201811449180 A CN201811449180 A CN 201811449180A CN 109247238 A CN109247238 A CN 109247238A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
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Abstract
The invention belongs to technical field of plant propagation, and in particular to a kind of five leaflet maple tissue culture outside sprout-cultivating-bottle radication methods, comprising the following steps: choose proliferation 40d, the good five leaflets maple tissue-cultured seedling of high 2~4cm upgrowth situation as outside sprout-cultivating-bottle material；By tissue culture plant inoculation in the induced medium of 1/2MS+10mg/L IBA+25g/L sucrose+5g/L agar, 2~10d of Fiber differentiation；By vermiculite and perlite mixing and after carbendazim sterilizes, it is fitted into the ventilative hole tray in bottom；Tissue-cultured seedling after taking Fiber differentiation cleans base portion, is inserted into matrix and compresses, one plant of every cave, insertion depth about 1cm, and blade face water spray covers hole tray lid, periodically sprays water, and 25d takes off lid, normal to conserve.Tissue culture outside sprout-cultivating-bottle radication method of the invention is simple, without washing root, avoids tissue-cultured seedling caused by washing root from damaging and pollute, rooting process of the invention is hardening process, shortens the seedling time, improves efficiency.
Description
Technical field
The invention belongs to technical field of plant propagation, and in particular to a kind of five leaflet maple tissue culture outside sprout-cultivating-bottle radication methods.
Background technique
About 200 kinds of global Aceraceae plant is distributed mainly on north temperate zone, is main product China and Japan, China has 150 kinds
More than, it is widely distributed in north and south each province, but distribution center is middle part or western part, some maples are good timber trees, some are very
Good nectariferous plant, some can be used as concealment tree and ornamental tree.Five leaflet maples are exactly rare ornamental tree.To so far,
Know that five leaflet maple fields are existing and only deposit more than 500 strains, five leaflet Acers are in minimum population, in " Chinese biological diversity Red List
Higher plant volume " inner it has been included in " pole danger " grade.2 months 2016, Sichuan provincial people's government disclosed 18 kinds of Sichuan Province's emphasis and protects
Wild plant register is protected, five leaflet maples are put into wherein.
Five leaflet maples (Acer Pentaphyllum) are Aceraceae Acer deciduous trees, can grow to 10 meters of height, but how long
To four or five meters of height, it is grown in the river valley area of height above sea level 2200-3000m.In Acer, five leaflet maples are that five leaflet maples system is unique
Kind.Autumn, five leaflet mapler leaf pieces by green flavescence, eventually became red, unique chicken feet shape is leaf and autumn is gorgeous more before this
The color of change makes it the object of global botanist and gardening scholar concern, and having person, it is " with the Acer palmatum ' Atropurpureum' world arranged side by side
Two maples ornamental greatly ".
Currently, the reproduction technique of five leaflet maples is mainly seed propagation, but germination is slow and germination percentage is low.At present
Also there are five leaflet maple branch rapid propagation methods of research, such as application No. is 201711422694.8 five leaflet maple branch of one kind is fast
The cultural method of speed breeding, comprising the following steps: step 1, acquisition triennial is hereinafter, tender tip length is the five leaflet maples of 4~6cm
Band bud branch is explant material；Step 2, band bud branch cut off blade, are cut into stem with bud, disappear to stem with bud
Poison；Step 3, stem with bud are placed in culture medium and cultivate, culture medium MS+NAA0.05mg/L+6-BA0.05mg/L+GA3
2.0mg/L+AC 1.0g/L；Step 4, strong seedling culture, culture medium are MS+GA3 1.0mg/L+NAA0.05mg/L+6-
BA0.05mg/L+AC 1.0g/L；Step 5, culture of rootage, culture medium White+IBA1.2mg/L+6-BA1.0mg/L+AC
1.0g/L；Step 6,7~15d after culture of rootage move to the tissue culture tank equipped with five leaflet maple seedlings in outdoor shade or greenhouse
It carries out closing tank 15~20d of hardening, shade density is 50%~70%；After closing tank hardening, then tissue culture cover is opened, in natural light
3~7d of lower progress can opening hardening；After hardening, five leaflet maple seedlings are removed with tweezers from tissue culture tank, cleans five leaflet maples
Seedling root；Step 7 is directly transplanted, or the liquor potassic permanganate using 0.1%~0.3% or clever bacterium solution cleaning more than 500 times
Five leaflet maple seedlings；Step 8 is periodically applied fertilizer after transplanting, carries out water and fertilizer management, and cultivation obtains five leaflet maple seedlings.But this method
The processes such as strong seedling culture, culture of rootage are needed, process is complicated, and the breeding cycle is long, and needs to clean five after step 6 hardening
Leaflet maple seedling root, cleaning process can cause seedling centainly to damage, if insufficient cleaning is even easy pollution death.Therefore,
It is badly in need of the five leaflet maple propagation methods that a kind of rooting rate is high, high survival rate and seedling time are short.
Summary of the invention
Present invention aims to overcome that above-mentioned the deficiencies in the prior art, the five of a kind of seedling time short, high rooting rate are provided
Leaflet maple tissue culture outside sprout-cultivating-bottle radication method.
To achieve the above object, the invention adopts the following technical scheme:
A kind of five leaflet maple tissue culture outside sprout-cultivating-bottle radication methods, comprising the following steps:
Step 1: prepare tissue-cultured seedling: choosing proliferation 40d, the good five leaflets maple tissue-cultured seedling of high 2~4cm upgrowth situation as bottle
External root timber material cuts off tissue-cultured seedling middle and lower part blade；
Step 2: tissue-cultured seedling rooting induction: by the tissue culture plant inoculation of step steady in induced medium, Fiber differentiation 2~
10d, the induced medium are 1/2MS+5~15mg/L IBA+25g/L sucrose+5g/L agar；
Step 3: prepare matrix: by vermiculite and perlite mixed-matrix after 800 times of carbendazim disinfection, it is ventilative to be packed into bottom
In hole tray, the amount for being packed into matrix is the 3/4 of hole tray height, is then sprinkled profoundly water, and is watered 1 time every 2-3 weeks using leaching basin method later；
Step 4: transplanting: the tissue-cultured seedling after taking the Fiber differentiation of step 2, clean base portion, inserting step three prepare matrix in
It compresses, one plant of every cave, insertion depth about 1cm, blade face water spray is primary, covers hole tray lid, water spray 2 times, 5~20d in 1~5d
Water spray is primary within one week, and 25d takes off lid, normal to conserve.
Further, the induced medium is 1/2MS+10mg/L IBA+25g/L sucrose+5g/L agar.
Further, the mass ratio of the vermiculite and perlite is 1~3:1.
Further, the mass ratio of the vermiculite and perlite is 3:1.
Further, Fiber differentiation described in step 2 is 2d.
Further, it is 22~27 DEG C that the tissue-cultured seedling transplanted in step 4, which is placed on temperature, the ring that humidity is 80% or more
In border.
The inorganic salt concentration of the MS culture medium is high, and especially ammonium salt and nitrate content is big, is able to satisfy and increases rapidly, adds
The effect of fast callus and culture growth reduces a great number of elements concentration in culture medium, can be improved in most plants
The rootability of test tube seedling, this research 1/2MS minimal medium are that MS minimal medium a great number of elements halves, other at
It is point constant, have inorganic salt concentration low, meets growth needs, be conducive to take root.
The IBA is indolebutyric acid, is a kind of auxin, is mainly used for rooting of cuttings, can induce root substance
It is formed, promotes cell differentiation and division, be conducive to the differentiation of new root generation and fibrovascular system, promote the shape of cutting adventitious root
At.
Five leaflet maple tissue culture seedling rooting situations of the invention are by exogenous hormone concentration, rooting induction time and tissue-cultured seedling physiology
State etc. influences, and the present invention uses IBA as exogenous hormone, by changing the content of endogenous hormones come indirect induction adventitious root
Occur and breaks up.High concentration exogenous hormone promotes tissue culture seedling rooting and root growth, but excessively high concentration then inhibits tissue-cultured seedling raw
Root and root growth；Hormone concentration is low, the rooting induction time is too short, then tissue-cultured seedling sucks a small amount of exogenous hormone, survives after transplanting
Rate is low, and rooting induction overlong time, and exogenous hormone too high levels play inhibiting effect to taking root for tissue-cultured seedling in tissue-cultured seedling；
In addition, there is also differences for response of the same plant difference growth conditions to hormone concentration.
Compared with prior art, the beneficial effects of the present invention are:
The present invention is by five leaflet maple tissue-cultured seedling after proliferation of propagation, after being inoculated into induced medium for a period of time, transplanting to matrix
In, root induction and domestication are carried out, tissue-cultured seedling is made gradually to adapt to external environment, the survival rate after improving rooting rate and transplanting.Together
When the present invention in transplantation rooting process be domestication process, shorten the seedling time.It is raw that the present invention saves existing tissue-cultured seedling
Root process is washed in method for transplanting after root, damage and insufficient cleaning caused by washing root is avoided and pollutes；Of the invention five
Leaflet maple tissue culture outside sprout-cultivating-bottle radication method, process is simple, easily operated, and rooting rate is high, improves the survival rate of five leaflet maples.
Detailed description of the invention
Fig. 1 is a kind of tissue-cultured seedling of five leaflet maple tissue culture outside sprout-cultivating-bottle radication methods of the invention；
Fig. 2 is a kind of tissue-cultured seedling rooting induction process of five leaflet maple tissue culture outside sprout-cultivating-bottle radication methods of the invention；
Fig. 3 is the rooted seedling after a kind of transplanting 40d of five leaflet maple tissue culture outside sprout-cultivating-bottle radication methods of the invention.
Specific embodiment
The following examples are intended to illustrate the invention, but is not used to limit the scope of protection of the present invention.Unless otherwise specified, real
Apply the conventional means that technological means used in example is well known to those skilled in the art.
Embodiment 1
A kind of five leaflet maple tissue culture outside sprout-cultivating-bottle radication methods, comprising the following steps:
Step 1: prepare tissue-cultured seedling: choosing proliferation 40d, the good five leaflets maple tissue-cultured seedling of high 2~4cm upgrowth situation as bottle
External root timber material cuts off tissue-cultured seedling middle and lower part blade；
Step 2: tissue-cultured seedling rooting induction: by the tissue culture plant inoculation of step steady in induced medium, Fiber differentiation 2d,
The induced medium is 1/2MS+10mg/L IBA+25g/L sucrose+5g/L agar；
Step 3: prepare matrix: being in mass ratio the dress after 3:1 is mixed after 800 times of carbendazim disinfection by vermiculite and perlite
Enter in the ventilative hole tray in bottom, the amount for being packed into matrix is the 3/4 of hole tray height, is then sprinkled profoundly water, and 40d is using leaching basin method watering one
Secondary, 60d is primary using leaching basin method watering；
Step 4: transplanting: the tissue-cultured seedling after taking the Fiber differentiation of step 2, clean base portion, inserting step three prepare matrix in
It compresses, one plant of every cave, insertion depth about 1cm, blade face water spray is primary, covers hole tray lid, the tissue-cultured seedling of transplanting is placed on temperature and is
22~27 DEG C, humidity is in 80% or more environment, water spray 2 times in 1~5d, water spray is primary within 5~20d mono- week, and 25d takes off
Lid is normal to conserve.
The induced medium of hormon concentration is to five leaflet maple tissue culture seedling rootings when 2 tissue-cultured seedling rooting induction of embodiment
It influences
Five leaflet maple tissue culture outside sprout-cultivating-bottle radication methods are referring to embodiment 1, by the dense of the hormone IBA in step 2 in induced medium
Degree is set as 0mg/L, 5mg/L, 10mg/L, 15mg/L, Fiber differentiation 4d, remaining step is identical.40d unites after step 4 transplanting
Rooting rate, indefinite radical and root long are counted, the results are shown in Table 1.
Rooting rate (%)=(tissue culture seedling rooting strain number/tissue-cultured seedling total strain number) × 100%；
Root long: every plant of tissue-cultured seedling maximum adventitious root length is denoted as to the root long of this plant of tissue-cultured seedling；
Influence of the induced medium of 1 hormon concentration of table to five leaflet maple tissue culture seedling rootings
As shown in Table 1: testing the induced medium to use 1/2MS+10mg/L IBA+25g/L sucrose+5g/L agar, induction
The five leaflet maple rooting efficiencies for cultivating 4d are best, and rooting rate reaches 91.67%, and indefinite radical is up to 3, root long 4.37cm.
Influence of the not isogeneous induction number of days of 3 tissue-cultured seedling rooting induction of embodiment to five leaflet maple tissue culture seedling rootings
Five leaflet maple tissue culture outside sprout-cultivating-bottle radication methods referring to embodiment 1, by Fiber differentiation number of days in step 2 be set as 0d, 2d,
4d, 6d, 8d, 10d, remaining step are identical.40d statistics rooting rate, indefinite radical and root long, the results are shown in Table after step 4 transplanting
2。
Influence of the 2 not isogeneous induction number of days of table to five leaflet maple tissue culture seedling rootings
| Induce number of days | Rooting rate % | Indefinite radical (item) | Root long cm |
| 0d(CK) | 53.33bc | 2.26a | 3.36a |
| 2d | 95.83a | 3.33a | 4.82a |
| 4d | 91.67a | 3a | 4.37a |
| 6d | 66.67b | 2.75a | 3.87a |
| 8d | 41.67c | 2.5a | 4.7a |
| 10d | 58.33bc | 3a | 3.5a |
As shown in Table 2: best with the five leaflet maple effects of rooting induction processing 2d, rooting rate reaches 95.83%, and indefinite radical reaches
3.33, root long 4.82cm.
Influence of 4 different substrates of embodiment to five leaflet maple tissue culture seedling rootings
Five leaflet maple tissue culture outside sprout-cultivating-bottle radication methods press matter referring to embodiment 1, by the matrix, that is, vermiculite and/or perlite of step 3
Amount is than being 0:1,1:0,1:1, and four groups of 3:1 setting, remaining step is identical.40d counts rooting rate, adventitious root after step 4 transplanting
Several and root long, the results are shown in Table 3.
Influence of 3 different substrates of table to five leaflet maple tissue culture seedling rootings
As shown in Table 3: matrix uses individual vermiculite or perlite, and five leaflet maple rooting efficiencies are bad, tests with vermiculite and treasure
Pearl rock mass ratio is that five leaflet maple effects of the matrix of 3:1 are best, and rooting rate reaches 95.83%, and indefinite radical is up to 3, root long
For 4.82cm, but it is not significant with the situation difference of taking root of the five leaflet maples with vermiculite and perlite mass ratio for the matrix of 1:1.
Influence of the five leaflet maple tissue-cultured seedling of 5 different height of embodiment to five leaflet maple tissue culture seedling rootings
Five leaflet maple tissue culture outside sprout-cultivating-bottle radication methods are referring to embodiment 1, outside sprout-cultivating-bottle material selection a height of 1.5cm, 2.5cm,
The good five leaflets maple tissue-cultured seedling of 3.5cm, 4.5cm upgrowth situation, remaining step are identical.40d statistics life after step 4 transplanting
Root rate, indefinite radical and root long, the results are shown in Table 4.
Influence of the tissue-cultured seedling of 4 different height of table to five leaflet maple tissue culture seedling rootings
As shown in Table 4: the five leaflet maple rooting rates of a height of 1.5cm are up to 95.83%, but indefinite radical is only 1.85.Therefore, it tries
Test best with the five leaflet maple effects of a height of 3.5cm, rooting rate reaches 91.67%, and indefinite radical is up to 3.06, root long
4.07cm。
The embodiment of the above, only presently preferred embodiments of the present invention, is only used to explain the present invention, not limit
The scope of the present invention processed to those of ordinary skill in the art certainly can be according to skill disclosed in this specification
Art content, makes other embodiments easily by way of replacing or changing, therefore all in the principle of the present invention and technique item
The changes and improvements etc. that part is done, should be included in scope of the present invention patent.
Claims (6)
1. a kind of five leaflet maple tissue culture outside sprout-cultivating-bottle radication methods, which comprises the following steps:
Step 1: prepare tissue-cultured seedling: choosing proliferation 40d, the good five leaflets maple tissue-cultured seedling of high 2~4cm upgrowth situation as bottle
External root timber material cuts off tissue-cultured seedling middle and lower part blade；
Step 2: tissue-cultured seedling rooting induction: by the tissue culture plant inoculation of step steady in induced medium, Fiber differentiation 2~
10d, the induced medium are 1/2MS+5~15mg/L IBA+25g/L sucrose+5g/L agar；
Step 3: prepare matrix: by vermiculite and perlite mixed-matrix after 800 times of carbendazim disinfection, it is ventilative to be packed into bottom
In hole tray, the amount for being packed into matrix is the 3/4 of hole tray height, is then sprinkled profoundly water, later every 2~3 weeks using leaching basin method watering 1
It is secondary；
Step 4: transplanting: the tissue-cultured seedling after taking the Fiber differentiation of step 2, clean base portion, inserting step three prepare matrix in
It compresses, one plant of every cave, insertion depth about 1cm, blade face water spray is primary, covers hole tray lid, water spray 2 times, 5~20d in 1~5d
Water spray is primary within one week, and 25d takes off lid, normal to conserve.
2. a kind of five leaflets maple tissue culture outside sprout-cultivating-bottle radication method according to claim 1, which is characterized in that the induction training
Supporting base is 1/2MS+10mg/L IBA+25g/L sucrose+5g/L agar.
3. a kind of five leaflets maple tissue culture outside sprout-cultivating-bottle radication method according to claim 1, which is characterized in that the vermiculite and
The mass ratio of perlite is 1~3:1.
4. a kind of five leaflets maple tissue culture outside sprout-cultivating-bottle radication method according to claim 1, which is characterized in that the vermiculite and
The mass ratio of perlite is 3:1.
5. a kind of five leaflets maple tissue culture outside sprout-cultivating-bottle radication method according to claim 1, which is characterized in that described in step 2
Fiber differentiation is 2d.
6. a kind of five leaflets maple tissue culture outside sprout-cultivating-bottle radication method according to claim 1, which is characterized in that moved in step 4
The tissue-cultured seedling of cultivation is placed in the environment that temperature is 22~27 DEG C, humidity is 80% or more.
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| CN201811449180.6A CN109247238B (en) | 2018-11-30 | 2018-11-30 | Acer negundo L tissue culture seedling ex-vitro rooting method |
| PCT/CN2019/093768 WO2020107888A1 (en) | 2018-11-30 | 2019-06-28 | Ex vitro rooting method for acer pentaphyllum tissue culture |
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| WO2020107888A1 (en) * | 2018-11-30 | 2020-06-04 | 四川七彩林科股份有限公司 | Ex vitro rooting method for acer pentaphyllum tissue culture |
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| CN115885844A (en) * | 2021-08-19 | 2023-04-04 | 大庆石油管理局有限公司 | Autumn flame rapid propagation method after northern domestication |
| CN114303780B (en) * | 2022-01-11 | 2023-04-11 | 郑英达 | Red maple planting method adopting container for seedling raising |
| CN114651659B (en) * | 2022-04-15 | 2023-05-23 | 山东省林业科学研究院 | Almond light matrix non-woven fabric container seedling raising method |
| CN116034876A (en) * | 2023-01-10 | 2023-05-02 | 重庆市铜梁区果之王园艺研究院 | GF677 peach stock culture medium and cultivation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104663450A (en) * | 2015-03-05 | 2015-06-03 | 罗焕荣 | Tissue culture and rapid propagation method for Acer rubrum 'Brandywine' |
| CN107455261A (en) * | 2017-09-15 | 2017-12-12 | 安徽农业大学 | A kind of method of Acer saccharum tissue culture quick breeding |
| CN107950398A (en) * | 2017-12-25 | 2018-04-24 | 绵阳师范学院 | The cultural method that a kind of five leaflets maple branch is quickly bred |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102511399B (en) * | 2012-01-13 | 2013-03-20 | 南京金埔园林股份有限公司 | Acer mono axillary bud path tissue culture method |
| CN108142284A (en) * | 2016-12-06 | 2018-06-12 | 四川七彩林业开发有限公司 | A kind of tissue culture and rapid propagation method of five leaflets maple |
| CN109247238B (en) * | 2018-11-30 | 2021-09-24 | 四川七彩林科股份有限公司 | Acer negundo L tissue culture seedling ex-vitro rooting method |
-
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104663450A (en) * | 2015-03-05 | 2015-06-03 | 罗焕荣 | Tissue culture and rapid propagation method for Acer rubrum 'Brandywine' |
| CN107455261A (en) * | 2017-09-15 | 2017-12-12 | 安徽农业大学 | A kind of method of Acer saccharum tissue culture quick breeding |
| CN107950398A (en) * | 2017-12-25 | 2018-04-24 | 绵阳师范学院 | The cultural method that a kind of five leaflets maple branch is quickly bred |
Non-Patent Citations (1)
| Title |
|---|
| 沙红: "五小叶槭育苗造林技术", 《林业科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020107888A1 (en) * | 2018-11-30 | 2020-06-04 | 四川七彩林科股份有限公司 | Ex vitro rooting method for acer pentaphyllum tissue culture |
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