CN102613078A - Method suitable for rapid rooting of blueberry tissue culture seedlings - Google Patents
Method suitable for rapid rooting of blueberry tissue culture seedlings Download PDFInfo
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- CN102613078A CN102613078A CN2012100835083A CN201210083508A CN102613078A CN 102613078 A CN102613078 A CN 102613078A CN 2012100835083 A CN2012100835083 A CN 2012100835083A CN 201210083508 A CN201210083508 A CN 201210083508A CN 102613078 A CN102613078 A CN 102613078A
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Abstract
The invention relates to a method suitable for rapid rooting of blueberry tissue culture seedlings, which includes the steps: cutting off single blueberry tissue culture seedlings which are cultured on rooting medium for 40-50 days and grow to more than 6-8cm, soaking the seedlings in rooting agent, performing cuttage to insert cuttings into feeding block in each bottle, and allowing the seedlings to grow a plurality of roots at the base in 18-22 days; removing a tissue culture seal film from the rooted tissue culture seedlings growing to more than 6-8cm, exercising the seedlings for 30 days, and directly transplanting the seedlings to a greenhouse. By the method, blueberry rooting culture is performed using the feeding blocks in the bottles initially, proper rooting medium and best rooting agent are selected, and accordingly an efficient blueberry tissue culture seedling rooting system is established. The method has the advantages that operation is simple, cost is low, the method is stable and efficient, seedling cycle is shortened greatly, and the like.
Description
Technical field
The present invention relates to the plant asexual multiplication technology field, relate in particular to the method that a kind of suitable blueberry tissue cultivating seedling is taken root fast.
Background technology
Blueberry (Vaccinium corymbosum) is an Ericaceae Vaccinium plant; Be perennial fallen leaves or evergreen shrubs; Fruit is a berry; Be high, the remarkable in economical benefits of a kind of value that at first grows up, have the nourishing healthy fruit of high nutritive value, health-care effect and medical value in the U.S..Blueberry is rich in anti-oxidant compounds such as various vitamins, ursin, anthocyanin, SOD, tanning acid and flavonoids, so it has unique health care.Blueberry simultaneously also is one of the most potential fruit tree species in the world wide in this century.
The blueberry fruit is the color very attractive not only, and meat exquisiteness, moderately sour and sweet, nutritious, fragrance salubrious, unique flavor, both can eat raw, also can be processed into multiple relevant food, liked by the consumer.It is reported; Blueberry is that anti-oxidant component content is the highest in all fruits and vegetables; Particularly contain abundant anthocyan in the fruit; To prevention thrombus and arteriosclerosis, anticancer, opposing urinary system infection contamination, prevention heart disease with delay senility and all have good effect, be a kind of health food very reliably, therefore also classified as one of human five big healthy food by international food and agricultural organization.
Along with the demand of blueberry is increasing, the nurseries growing tension adopts traditional propagation methods such as seedling grafting, branch cutting, has slow, the problem such as survival rate is low, new talent is of poor quality, growing-seedling period is long of taking root.And adopt method for plant tissue culture can breed the blueberry seedling in a short time fast, not only it is vegetative propagation, can keep the merit of former stock, and has the high advantage of reproduction rate.Rooting rate is low, the cycle of taking root is long, the program of taking root is complicated and the problems such as survival rate is low of transplanting seedlings but have in the blueberry stage of reporting in the existing document of taking root, and it can't large-scale industrializedly be produced.
Summary of the invention
The technical problem that the present invention will solve provides a kind of simple to operate, with low cost, stability and high efficiency, and the suitable blueberry tissue cultivating seedling that growing-seedling period is shortened the greatly method of taking root fast.
1), in tissue culture bottle, put into a small amount of stone for solving the problems of the technologies described above, the present invention adopts following technical scheme: include following steps:, put into one of nutritive cube again, and seal film with the group training and seal; 2), the blueberry tissue cultivating seedling carried out in the bottle in the nutritive cube cuttage root-taking after with the root-growing agent immersion treatment cultivate, obtain the seedling of taking root; 3), the blueberry tissue cultivating seedling that reaches more than the 6-8cm of the growth that will cultivate on the matrix of taking root 40-50 days cuts by individual plant, soak root-growing agent after, in the nutritive cube, the seedling base portion grew several root systems in 18-22 days in the bottle in cuttage; 4), growth in the step 3) is reached the tissue cultivating seedling of taking root more than the 6-8cm, open group training and seal film, refining seedling 30 days, direct transplanting is in green house again; Wherein, the compound method of the said root-growing agent of 1L is following: earlier with the NaOH dissolving of a small amount of 1mol/L, water is settled to 1L again with the 10mg indolebutyric acid; The said matrix of taking root is formulated by the peat composed of rotten mosses and perlite 3: 1 by volume, and subsequent use with 70 ℃ of sterilizations of baking oven 1h cooling.
Further, the top edge of said nutritive cube exceeds tissue culture bottle mouth 4-6mm.
Further, said tissue culture bottle and stone need preliminary treatment, need in baking oven 70 ℃ of sterilizations 1 hour, use after cooling.
Further, the time that said tissue cultivating seedling soaks root-growing agent is 2 minutes, soaks cutting base portion 2-3cm.
Further, tissue cultivating seedling was one every bottle when said cuttage root-taking was cultivated, and the cuttage degree of depth is 2-3cm.
Further, when culture of rootage, sprayed one time 0.1% carbendazim in per three days, with drenched being advisable of stone of tissue culture bottle bottom.
Further, the condition of culture in described culture of rootage stage is, cultivation temperature is 25 ± 2 ℃, and light intensity is 2000-2500Lx, and light application time is 14h/d, and refining seedling stage humidity is 70%.
The method that a kind of suitable blueberry tissue cultivating seedling of the present invention is taken root fast; This method is carried out the blueberry culture of rootage through nutritive cube in the bottle for the first time; And, set up a kind of system of taking root of the tissue cultivating seedling of blueberry efficiently through selecting suitable take root matrix and best root-growing agent.Have simple to operate, with low cost, stability and high efficiency, and make advantages such as growing-seedling period shortens greatly.
Embodiment
1), in tissue culture bottle, put into a small amount of stone the method that a kind of suitable blueberry tissue cultivating seedling is taken root fast includes following steps:, put into one of nutritive cube again, and seal film with the group training and seal; 2), the blueberry tissue cultivating seedling carried out in the bottle in the nutritive cube cuttage root-taking after with the root-growing agent immersion treatment cultivate, obtain the seedling of taking root; 3), the blueberry tissue cultivating seedling that reaches more than the 6-8cm of the growth that will cultivate on the matrix of taking root 40-50 days cuts by individual plant, soak root-growing agent after, in the nutritive cube, the seedling base portion grew several root systems in 18-22 days in the bottle in cuttage; In the present embodiment, the seedling base portion grew several root systems in 20 days.4), growth in the step 3) is reached the tissue cultivating seedling of taking root more than the 6-8cm, open group training and seal film, refining seedling 30 days, direct transplanting is in green house again; Wherein, the compound method of the said root-growing agent of 1L is following: earlier with the NaOH dissolving of a small amount of 1mol/L, water is settled to 1L again with the 10mg indolebutyric acid; The said matrix of taking root is formulated by the peat composed of rotten mosses and perlite 3: 1 by volume, and subsequent use with 70 ℃ of sterilizations of baking oven 1h cooling.
Wherein, the top edge of said nutritive cube exceeds tissue culture bottle mouth 4-6mm.Said tissue culture bottle and stone need preliminary treatment, need in baking oven 70 ℃ of sterilizations 1 hour, use after cooling.The time that said tissue cultivating seedling soaks root-growing agent is 2 minutes, soaks cutting base portion 2-3cm.Tissue cultivating seedling was one every bottle when said cuttage root-taking was cultivated, and the cuttage degree of depth is 2-3cm.When culture of rootage, sprayed one time 0.1% carbendazim in per three days, with drenched being advisable of stone of tissue culture bottle bottom.The condition of culture in described culture of rootage stage is, cultivation temperature is 25 ± 2 ℃, and light intensity is 2000-2500Lx, and light application time is 14h/d, and refining seedling stage humidity is 70%.
The invention has the beneficial effects as follows:
1), nutritive cube reaches more than 90% rooting rate to blueberry pointed strong, the characteristics such as applicability is good of taking root in the bottle, rootage duration foreshortens to 20 days, and transplanting survival rate reaches 100%.
2), this method has been owing to utilized bottle an interior nutritive cube to carry out culture of rootage, humidity keeps constant in the bottle, can not consider a bottle outer humidity, the input of special equipments such as minimizing spraying humidification.
3), this method has been owing to utilized nutritive cube to be the cuttage container, but take root uncap after direct transplanting in green house, thereby improved transplanting survival rate, and shortened adaptation time, increase work efficiency.
4), the present invention is owing to utilize in the bottle nutritive cube to carry out culture of rootage under the condition of bacterium having; Saved this link of moist heat sterilization than taking root in the bottle under the aseptic condition; Reduce cost, reduced labour intensity, had characteristics such as quick, economic, succinct, efficient; Increased substantially the reproduction speed and the quality of blueberry seedling, provided safeguard thereby breed the blueberry seedling for large-scale commercial.
The method that a kind of suitable blueberry tissue cultivating seedling of the present invention is taken root fast; This method is carried out the blueberry culture of rootage through nutritive cube in the bottle for the first time; And, set up a kind of system of taking root of the tissue cultivating seedling of blueberry efficiently through selecting suitable take root matrix and best root-growing agent.Have simple to operate, with low cost, stability and high efficiency, and make advantages such as growing-seedling period shortens greatly.
Below the present invention is done a detailed description, the above is merely the present invention's preferred embodiment, when can not limiting practical range of the present invention, promptly allly does impartially to change and modify according to the application's scope, all should still belong in the covering scope of the present invention.
Claims (7)
1. method that suitable blueberry tissue cultivating seedling is taken root fast includes following steps: 1), in tissue culture bottle is given birth to, put into a small amount of stone, put into one of nutritive cube again, and train with group and to seal film and seal; 2), the blueberry tissue cultivating seedling carried out in the bottle in the nutritive cube cuttage root-taking after with the root-growing agent immersion treatment cultivate, obtain the seedling of taking root; 3), the blueberry tissue cultivating seedling that reaches more than the 6-8cm of the growth that will cultivate on the matrix of taking root 40-50 days cuts by individual plant, soak root-growing agent after, in the nutritive cube, the seedling base portion grew several root systems in 18-22 days in the bottle in cuttage; 4), growth in the step 3) is reached the tissue cultivating seedling of taking root more than the 6-8cm, open group training and seal film, refining seedling 30 days, direct transplanting is in green house again; Wherein, the compound method of the said root-growing agent of 1L is following: earlier with the NaOH dissolving of a small amount of 1mol/L, water is settled to 1L again with the 10mg indolebutyric acid; The said matrix of taking root is formulated by the peat composed of rotten mosses and perlite 3: 1 by volume, and subsequent use with 70 ℃ of sterilizations of baking oven 1h cooling.
2. the method that suitable blueberry tissue cultivating seedling according to claim 1 is taken root fast is characterized in that: the top edge of said nutritive cube exceeds tissue culture bottle mouth 4-6mm.
3. the method that suitable blueberry tissue cultivating seedling according to claim 1 is taken root fast is characterized in that: said tissue culture bottle and stone need preliminary treatment, need in baking oven 70 ℃ of sterilizations 1 hour, use after cooling.
4. the method that suitable blueberry tissue cultivating seedling according to claim 1 is taken root fast is characterized in that: the time that said tissue cultivating seedling soaks root-growing agent is 2 minutes, soaks cutting base portion 2-3cm.
5. the method that suitable blueberry tissue cultivating seedling according to claim 1 is taken root fast is characterized in that: tissue cultivating seedling was one every bottle when said cuttage root-taking was cultivated, and the cuttage degree of depth is 2-3cm.
6. the method that suitable blueberry tissue cultivating seedling according to claim 1 is taken root fast is characterized in that: when culture of rootage, sprayed one time 0.1% carbendazim in per three days, with drenched being advisable of stone of tissue culture bottle bottom.
7. the method that suitable blueberry tissue cultivating seedling according to claim 1 is taken root fast; It is characterized in that: the condition of culture in described culture of rootage stage is, cultivation temperature is 25 ± 2 ℃, and light intensity is 2000-2500Lx; Light application time is 14h/d, and refining seedling stage humidity is 70%.
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| CN2012100835083A CN102613078A (en) | 2012-03-27 | 2012-03-27 | Method suitable for rapid rooting of blueberry tissue culture seedlings |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103636487A (en) * | 2013-12-27 | 2014-03-19 | 张建锋 | Rooting method using system with upper and lower liquid outlet holes and electronic control device |
| CN103636373A (en) * | 2013-11-04 | 2014-03-19 | 大连大学 | Method for vegetative propagation of vaccinium plants through fresh sphagnum |
| CN103651090A (en) * | 2013-12-27 | 2014-03-26 | 杨健儿 | Rooting method adopting system with liquid outlet plates and electronic control device |
| CN103718938A (en) * | 2013-12-27 | 2014-04-16 | 孟祖钢 | Rooting method using system with upper liquid draining holes, lower liquid draining holes and pressure boosting device |
| CN103828721A (en) * | 2014-03-20 | 2014-06-04 | 北京农学院 | Application of bacillus licheniformis to promotion of rooting or growth of semen trigonellae tissue culture seedlings |
| CN104430359A (en) * | 2014-10-23 | 2015-03-25 | 湖南省核农学与航天育种研究所 | Composite rooting powder as well as preparation method and application thereof |
| CN104430359B (en) * | 2014-10-23 | 2017-01-04 | 湖南省核农学与航天育种研究所 | A kind of compound take root powder and compound method thereof and application |
| CN106613962A (en) * | 2016-11-14 | 2017-05-10 | 宁波市农业科学研究院 | Culture medium for rooting of tissue-cultured blueberry seedling and culturing method using same |
| CN108668659A (en) * | 2018-07-18 | 2018-10-19 | 苏州枫彩生态科技集团有限公司 | A kind of method of the outer cuttage root-taking of blueberry bottle |
| CN110741931A (en) * | 2019-10-15 | 2020-02-04 | 贵州省生物研究所 | Preparation method of culture medium for promoting tissue culture and rooting of blueberries |
-
2012
- 2012-03-27 CN CN2012100835083A patent/CN102613078A/en active Pending
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103636373A (en) * | 2013-11-04 | 2014-03-19 | 大连大学 | Method for vegetative propagation of vaccinium plants through fresh sphagnum |
| CN103636487B (en) * | 2013-12-27 | 2015-04-08 | 张建锋 | Rooting method using system with upper and lower liquid outlet holes and electronic control device |
| CN103651090A (en) * | 2013-12-27 | 2014-03-26 | 杨健儿 | Rooting method adopting system with liquid outlet plates and electronic control device |
| CN103718938A (en) * | 2013-12-27 | 2014-04-16 | 孟祖钢 | Rooting method using system with upper liquid draining holes, lower liquid draining holes and pressure boosting device |
| CN103636487A (en) * | 2013-12-27 | 2014-03-19 | 张建锋 | Rooting method using system with upper and lower liquid outlet holes and electronic control device |
| CN103718938B (en) * | 2013-12-27 | 2015-03-11 | 孟祖钢 | Rooting method using system with upper liquid draining holes, lower liquid draining holes and pressure boosting device |
| CN103651090B (en) * | 2013-12-27 | 2015-04-08 | 杨健儿 | Rooting method adopting system with liquid outlet plates and electronic control device |
| CN103828721B (en) * | 2014-03-20 | 2016-03-02 | 北京农学院 | Bacillus licheniformis is promoting the application in blueberry tissue culture seedling rooting or growth |
| CN103828721A (en) * | 2014-03-20 | 2014-06-04 | 北京农学院 | Application of bacillus licheniformis to promotion of rooting or growth of semen trigonellae tissue culture seedlings |
| CN104430359A (en) * | 2014-10-23 | 2015-03-25 | 湖南省核农学与航天育种研究所 | Composite rooting powder as well as preparation method and application thereof |
| CN104430359B (en) * | 2014-10-23 | 2017-01-04 | 湖南省核农学与航天育种研究所 | A kind of compound take root powder and compound method thereof and application |
| CN106613962A (en) * | 2016-11-14 | 2017-05-10 | 宁波市农业科学研究院 | Culture medium for rooting of tissue-cultured blueberry seedling and culturing method using same |
| CN108668659A (en) * | 2018-07-18 | 2018-10-19 | 苏州枫彩生态科技集团有限公司 | A kind of method of the outer cuttage root-taking of blueberry bottle |
| CN110741931A (en) * | 2019-10-15 | 2020-02-04 | 贵州省生物研究所 | Preparation method of culture medium for promoting tissue culture and rooting of blueberries |
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Application publication date: 20120801 |