CN102823495A - Culture method for blueberries - Google Patents
Culture method for blueberries Download PDFInfo
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- CN102823495A CN102823495A CN2012103305534A CN201210330553A CN102823495A CN 102823495 A CN102823495 A CN 102823495A CN 2012103305534 A CN2012103305534 A CN 2012103305534A CN 201210330553 A CN201210330553 A CN 201210330553A CN 102823495 A CN102823495 A CN 102823495A
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Abstract
The invention discloses a culture method for blueberries. The method comprises the following concrete steps that (1) before the blueberries are planted, organic fertilizers are uniformly spread on the surface of the soil, the deep turning is carried out for mixing the organic fertilizers into the soil surface layer, and then, sawdust or corn straws are used for coverage; (2) strongly growing new branches are selected, leaves are sheared off, tap water is used for cleanly flushing the branches, then, sterilization is carried out, sterile water is used for rinsing for 3 to 6 times, and in addition, the branches are soaked for 20 minutes in the sterile water; the stem section is sheared into small sections with one to two buds; the small sections are inoculated onto a culture medium for multiplication culture for four weeks, the same method is used for subculture for four times; the stem section base part is inserted into a triangular culture bottle containing rooting substrate, and the temperature, the humidity and the light period in a culture room are controlled; and the transplanting is carried out after one month. When the method provided by the invention is adopted, the rooting rate reaches higher than 90 percent, the rooting time is shortened, the transplanting survival rate reaches 100 percent, and in addition, the speed and the quality of the blueberry seed seedling propagation are greatly and respectively accelerated and improved by the method provided by the invention, so the guarantee is provided for the large-scale commercialized blueberry seed seedling propagation culture.
Description
Technical field
The present invention relates to the kind method for planting of a plant species, specifically, relate to a kind of breeding method of blueberry.
Background technology
Blueberry formal name used at school cowberry belongs to Ericaceae (Ericaceae) Vaccinium (Vaccinium spp.) plant.The blueberry taste of fruit is unique, and is nutritious, is described as the king of berry.The blueberry fruit is the color very attractive not only, and meat exquisiteness, moderately sour and sweet, nutritious, fragrance salubrious, unique flavor, both can eat raw, also can be processed into multiple relevant food, liked by the consumer.It is reported; Blueberry is that anti-oxidant component content is the highest in all fruits and vegetables; Particularly contain abundant anthocyan in the fruit; To prevention thrombus and arteriosclerosis, anticancer, opposing urinary system infection contamination, prevention heart disease with delay senility and all have good effect, be a kind of health food very reliably, therefore also classified as one of human five big healthy food by international food and agricultural organization.
Along with the demand of blueberry is increasing, the nurseries growing tension, though the artificial cultivation of blueberry T&B so far, because of its development rapidly, supply falls short of demand for the blueberry nursery stock, become the technical bottleneck of the national blueberry industry development of restriction.Adopt traditional propagation methods such as seedling grafting, branch cutting, have slow, the problem such as survival rate is low, new talent is of poor quality, growing-seedling period is long of taking root.And adopt method for plant tissue culture can breed the blueberry seedling in a short time fast, not only it is vegetative propagation, can keep the merit of former stock, and has the high advantage of reproduction rate.Rooting rate is low, the cycle of taking root is long, the program of taking root is complicated and the problems such as survival rate is low of transplanting seedlings but have in the blueberry stage of reporting in the existing document of taking root, and it can't large-scale industrializedly be produced.
Simultaneously, it is higher that regional most soil pH values of blueberry can be cultivated by China, need carry out soil melioration.At present, during the plantation blueberry, generally adopt the Cosan and the acid peat composed of rotten mosses that the pH value of soil is reduced to proper scope,, cause the resilience of pH value easily, cause soil melioration not in place though this method can be improved the acid-base value of soil effectively.Simultaneously because a little less than the root system of blueberry; Absorbing capacity is little; Adopt traditional propagation method and conventional group cultivation seedlings such as branch cutting; All there is the problem slow, that survival rate is low, new talent is of poor quality, growing-seedling period is long of taking root, causes the PR of blueberry lower, seriously hindered blueberry further developing in China.
Summary of the invention
The objective of the invention is to the deficiency to prior art, a kind of blueberry breeding method is provided, this method can make the growing-seedling period of blueberry shorten greatly, can also guarantee pH value stabilization, the plant percent height of soil.
Technical scheme of the present invention is: a kind of breeding method of blueberry, and its concrete steps are:
(1) soil melioration
Before the blueberry plantation, soil was improved in 3-4 month; Wherein, Improve the soil mainly is the fertilizer after the fermentation evenly to be dispersed in need soil surface; Plough deeply and admix in the upper soll layer, cover and accomplish soil is improved with saw wood ash or maize straw then, the pH value of soil is reduced to 4.3～5.3;
(2) grow seedlings
Select eugonic shoot, cut off blade, the stem section is rinsed back sterilization well with running water and with rinsed with sterile water 3～6 times, and in sterile water, soaks 20min; Take out at last that the stem section is cut is the segment of 1～2 bud; Then above-mentioned segment is seeded on the medium around the enrichment culture, obtains a large amount of young sprouts, subculture 4 times uses the same method; Then stem segment base portion is inserted and fill in the triangle blake bottle of the matrix of taking root temperature, humidity and photoperiod in the control culturing room; Transplanted later in one month;
Above-mentioned medium is: WPM+ZT 0.5～1.5mg/L+CPPU0.05～1.0mg/L+ sucrose 30g/L+ agar 6.5g/L, and pH5.4 transfers pH to 5.2～5.4, autoclave sterilization 15min;
The main component of the above-mentioned matrix of taking root is 500ppmIAA growth hormone and 2.0mM glycinebetaine.
Also added the phosphate buffer of pH value in the above-mentioned matrix of taking root for 4.5-5.3.
The invention has the beneficial effects as follows: (1) adopts the present invention that rooting rate is reached more than 90%, and rootage duration shortens, and transplanting survival rate reaches 100%.(2) because the present invention carries out culture of rootage in bottle, humidity keeps constant in the bottle, can not consider the outer humidity of bottle, reduces the input of special equipments such as spraying humidification.(3) because having under the condition of bacterium, the present invention carries out culture of rootage in bottle; Saved this link of moist heat sterilization than taking root in the bottle under the aseptic condition; Reduce cost, reduced labour intensity, had characteristics such as quick, economic, succinct, efficient; Increased substantially the reproduction speed and the quality of blueberry seedling, provided safeguard thereby breed the blueberry seedling for large-scale commercial.
Embodiment
A kind of breeding method of blueberry, press following step:
(1) soil melioration
When blueberry is planted preceding 4 months, the fertilizer after the fermentation evenly is dispersed in the soil surface that needs, plough deeply and admix in the upper soll layer, cover and accomplish soil is improved with saw wood ash or maize straw then;
(2) grow seedlings
Select eugonic shoot, cut off blade, the stem section is rinsed back sterilization well with running water and with rinsed with sterile water 5 times, and in sterile water, soaks 20min; Take out at last the stem section is cut the segment into 1-2 bud; Then above-mentioned segment is seeded on the medium around the enrichment culture, obtains a large amount of young sprouts, subculture 4 times uses the same method; Then above-mentioned stem segment base portion is inserted and fill in the triangle blake bottle of the matrix of taking root temperature, humidity and photoperiod in the control culturing room; Transplanted later in one month;
Above-mentioned medium is: WPM+ZT 0.5～1.5mg/L+CPPU0.05～1.0mg/L+ sucrose 30g/L+ agar 6.5g/L, and pH5.4 transfers pH to 5.2～5.4, autoclave sterilization 15min;
The main component of the above-mentioned matrix of taking root is 500ppmIAA growth hormone and 2.0mM glycinebetaine.
Also added the phosphate buffer of pH value in the above-mentioned matrix of taking root for 4.5-5.3.
Claims (4)
1. the breeding method of a blueberry is characterized in that its concrete steps are:
(1) soil melioration
Before the blueberry plantation, soil was improved in 3-4 month; Wherein, Improve the soil mainly is the fertilizer after the fermentation evenly to be dispersed in need soil surface; Plough deeply and admix in the upper soll layer, cover and accomplish soil is improved with saw wood ash or maize straw then, the pH value of soil is reduced to 4.3～5.3;
(2) grow seedlings
Select eugonic shoot, cut off blade, the stem section is rinsed back sterilization well with rinsed with sterile water 3～6 times with running water, and in sterile water, soaks 20min; It is the segment of 1～2 bud that the stem section is cut in taking-up; Then above-mentioned segment is seeded on the medium around the enrichment culture, obtains a large amount of young sprouts, subculture 4 times uses the same method; Then stem segment base portion is inserted and fill in the triangle blake bottle of the matrix of taking root temperature, humidity and photoperiod in the control culturing room; Transplanted later in one month.
2. the breeding method of blueberry according to claim 1; It is characterized in that said medium is: WPM+ZT 0.5～1.5mg/L+CPPU0.05～1.0mg/L+ sucrose 30g/L+ agar 6.5g/L; PH5.4 transfers pH to 5.2～5.4, autoclave sterilization 15min.
3. the breeding method of blueberry according to claim 1, the main component that it is characterized in that the described matrix of taking root is 500ppmIAA growth hormone and 2.0mM glycinebetaine.
4. the breeding method of blueberry according to claim 1 is characterized in that also adding in the said matrix of taking root the phosphate buffer of pH value for 4.5-5.3.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2012103305534A CN102823495A (en) | 2012-09-10 | 2012-09-10 | Culture method for blueberries |
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| CN2012103305534A CN102823495A (en) | 2012-09-10 | 2012-09-10 | Culture method for blueberries |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103875532A (en) * | 2014-03-25 | 2014-06-25 | 江苏农林职业技术学院 | Proliferation culture medium for tissue culture of Jietu vaccinium vitisidaea |
| CN104429794A (en) * | 2014-12-15 | 2015-03-25 | 杨忠华 | Grape planting method |
| CN104488640A (en) * | 2014-11-27 | 2015-04-08 | 广德县桃州镇横山中路乐府肥羊 | Cultivating method for large seedling of blueberry during transplantation period |
| CN106305385A (en) * | 2016-08-19 | 2017-01-11 | 江苏艺轩园林景观工程有限公司 | Culture method of blueberry |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101869078A (en) * | 2010-07-14 | 2010-10-27 | 长春百瑞蓝莓科技发展有限公司 | Seed-cultivating method of blueberry by means of tissue cultivation and micropropagation |
| CN101904286A (en) * | 2009-06-05 | 2010-12-08 | 董文卓 | Method for growing blueberry |
| CN102630457A (en) * | 2012-04-17 | 2012-08-15 | 云南省农业科学院高山经济植物研究所 | High-efficiency cultivation method for blueberry |
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2012
- 2012-09-10 CN CN2012103305534A patent/CN102823495A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101904286A (en) * | 2009-06-05 | 2010-12-08 | 董文卓 | Method for growing blueberry |
| CN101869078A (en) * | 2010-07-14 | 2010-10-27 | 长春百瑞蓝莓科技发展有限公司 | Seed-cultivating method of blueberry by means of tissue cultivation and micropropagation |
| CN102630457A (en) * | 2012-04-17 | 2012-08-15 | 云南省农业科学院高山经济植物研究所 | High-efficiency cultivation method for blueberry |
Non-Patent Citations (1)
| Title |
|---|
| 黄文江等: "高灌蓝莓离体繁殖的研究", 《安徽师范大学学报（自然科学版）》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103875532A (en) * | 2014-03-25 | 2014-06-25 | 江苏农林职业技术学院 | Proliferation culture medium for tissue culture of Jietu vaccinium vitisidaea |
| CN104488640A (en) * | 2014-11-27 | 2015-04-08 | 广德县桃州镇横山中路乐府肥羊 | Cultivating method for large seedling of blueberry during transplantation period |
| CN104429794A (en) * | 2014-12-15 | 2015-03-25 | 杨忠华 | Grape planting method |
| CN106305385A (en) * | 2016-08-19 | 2017-01-11 | 江苏艺轩园林景观工程有限公司 | Culture method of blueberry |
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Application publication date: 20121219 |