CN100998314B - Method for tissue culture quick breeding of sea-buckthorn - Google Patents
Method for tissue culture quick breeding of sea-buckthorn Download PDFInfo
- Publication number
- CN100998314B CN100998314B CN2006100001940A CN200610000194A CN100998314B CN 100998314 B CN100998314 B CN 100998314B CN 2006100001940 A CN2006100001940 A CN 2006100001940A CN 200610000194 A CN200610000194 A CN 200610000194A CN 100998314 B CN100998314 B CN 100998314B
- Authority
- CN
- China
- Prior art keywords
- root
- culture
- medium
- seedling
- seabuckthorn
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Abstract
A tissue culture method for fast reproducing seabuckthorn, belonging to the field of plant tissue culture, includes such steps as choosing round seeds of seabuckthorn, disinfecting, germinating in germinating culture medium until the euphylla is fully grown, taking its stem tip (1.5-2.0 cm), reproductive culturing in the culture medium WPM containing BA (0.3-0.6 mg/L), taking healthy seedling with a length of 3-4 cm, outdoor rooting culturing in the culture medium 1/4 SH containing IBA (0.3-0.6 mg/L), NAA (0.03-0.06 mg/L) and AC of 1.9 g, and transplanting when the length of the root is 3-6 cm. The tissue culture method for fast reproducing seabuckthorn has the advantages of inducing seabuckthorn seedings in test tubes to take reproductive culturing rapidly to generate a plenty of adventitious buds with a 80% survival rate, and providing an efficient way for culturing seabuckthorn seedings on an enlarged scale.
Description
Technical field
The present invention relates to the tissue culture and rapid propagation method of a kind of sea-buckthorn, belong to the Plant Tissue Breeding field.
Background technology
Sea-buckthorn (Hippophae rhamnoides L.) is Elaeangnaceae (Elaeagnaceae) Hippophne (Hippophae L.) perennial deciduous fruit tree, shrub or a dungarunga.Its fruit, pericarp, blade, bark and fabricated product thereof as containing 280 several physiological active substances in fruit juice, pomace, the fruit oil, have the special efficacy of strong anti-inflammatory, sterilization, pain relieving and promotion regeneration.Wherein many compositions kill and suppress tumour cell, radioresistance, anticoagulation, hypotensive, prevent that aspects such as blood vessel embolism, anti-ageing, antifatigue, enhancing human body vigor and immunity from all demonstrating magical result of treatment, is described as " green gold ".
The sea-buckthorn branches and leaves are luxuriant simultaneously, the lateral root prosperity, and root turion is extremely strong, and the root of going here and there rapidly forms intensive colony from tiller, and can form a large amount of root nodules with symbiosis such as actinomycetes, bacterium, mycobacteriums, has the nitrogen fixing capacity stronger than soybean (but per hectare fixed nitrogen 180kg).In addition, its growth rapidly, and is drought-resistant, salt tolerant alkali is barren, drives in freezing winter and in sultry summer, has to conserve water and soil, check winds and fix drifting sand, improve the soil and the adaptability obvious effect that promotes the ecological balance such as strong, being not only the pioneer tree species of dry wind sand ground district's afforestation, also is important firewood forest and fodder forest seeds.Therefore, in current conceding the land to forestry, agricultural industry restructuring, the plantation sea-buckthorn has important nutritive value, the ecological value and economic worth.
At present, sea-buckthorn mainly adopt be grow directly from seeds, grafting (bud grafting) and cuttage and seedling culture, but also division propagation.But because sea-buckthorn is dioecian plant, the offspring of seminal propagation easily morphs, and the seedling sex is difficult to distinguish, can only be used for the construction of the ecological forest.And carry out vegetative propagation with seedling cuttage, grafting and root turion, and exist that planting percent is low, speed waits shortcoming slowly, be difficult to adapt to the demand of market to nursery stock.Continuous expansion along with the sea-buckthorn cultivated area, for bury having drought resisting, cold-resistant, salt tolerant alkali, desertification in satisfy producing, the wilderness demand of the new lines nursery stock of disease-resistant worm, output height, merit such as big fruit is stingless, the in-vitro propagate technology of sea-buckthorn is existing a large amount of reports in short more than ten years, but its sprouting starts slow, survival rate is low, and reproduction coefficient is not high.Axillary root is arranged can seeing on the fast breeding culture medium on the regeneration plant, can independently survive after the plant division, but poor growth.
Summary of the invention
Main purpose of the present invention is to overcome in the sea-buckthorn group training process explant to start slow, survival rate and the low deficiency of reproduction coefficient, and the quick breeding method for tissue culture of a kind of sea-buckthorn is provided.
Technical scheme of the present invention is: (1) selects full hippophae rhamnoides seed, is placed on after the surface sterilization on the germination medium and germinates; The germination medium mainly comprises 6.5g/L agar powder and 30g/L white granulated sugar.(2) treat that true leaf grows fully after, clip 1.5～2.0cm stem apex carries out enrichment culture, minimal medium is WPM medium (Woody Plant Medium is hereinafter to be referred as WPM), mainly comprises following composition: NH
4NO
3400mg/L, Ca (NO
3)
24H
2O 556mg/L, CaCl
22H
2O 96mg/L, MgSO
47H
2O 370mg/L, KH
2PO
4170mg/L, K
2SO
4990mg/L, H
3BO
36.2mg/L, MnSO
4H
2O 17.7mg/L, ZnSO
47H
2O 8.6mg/L, Na
2MoO
42H
2O 0.25mg/L, CuSO
45H
2O 0.25mg/L, FeSO
47H
2O 27.8mg/L, Na
2-EDTA 37.3mg/L, inositol 100mg/L, glycine 2mg/L, nicotinic acid 0.5mg/L, thiamine hydrochloride 1.0mg/L, puridoxine hydrochloride 0.5mg/L; Additional plant hormone is the 6-benzylaminopurine (benzylaminopurine is hereinafter to be referred as BA) of 0.3～0.6mg/L; Also comprise 6.5g/L agar powder and 30g/L white granulated sugar.(3) single seedling of clip robust growth, long 3～4cm carries out culture of rootage, and minimal medium is 1/4SH medium (SH medium macroelement reduces 3/4, and other elements remain unchanged, hereinafter to be referred as 1/4SH), mainly comprises following composition: NH
4H
2PO
475mg/L, KNO
3625mg/L, CaCl
22H
2O 50mg/L, MgSO
47H
2O 100mg/L, KI 1mg/L, H
3BO
35.0mg/L, MnSO
4H
2O 7.6mg/L, ZnSO
47H
2O 1mg/L, Na
2MoO
42H
2O 0.1mg/L, CuSO
45H
2O 0.2mg/L, CoCl
26H
2O 0.1mg/L, FeSO
47H
2O 15mg/L, Na
2-EDTA 20mg/L, inositol 1000mg/L, nicotinic acid 5.0mg/L, thiamine hydrochloride 5.0mg/L, puridoxine hydrochloride 0.5mg/L; Additional plant hormone is the indolebutyric acid (indolebutyric acid is hereinafter to be referred as IBA) of 0.3～0.6mg/L, the methyl of 0.03～0.06mg/L (naphthylene acetic acid is hereinafter to be referred as NAA); Also comprise 1.0g/L active carbon (activated charcoal is designated hereinafter simply as AC), 7.0g/L agar and 15g/L white granulated sugar, pH5.8; Cultivation temperature is that 25 ± 2 ℃, intensity of illumination are 1500～2000lx, and the photoperiod is 18h/d.It is long to add up its propagation multiple, average plant height, rooting rate, mean elements and average root when being cultured to 50 days.(4) when root continues to grow to 3～6cm, will seal film and open, hardening 3～6 days.Earlier press from both sides out seedling gently with tweezers, gently clean root medium in flowing water, soak 10min with 0.01% potassium permanganate again, transplant (perlite: vermiculite=3: 1) to the nutrient cup that matrix is housed, set upright seedling, shoot root is unfolded as far as possible, keep 20～25 ℃ of temperature, humidity 80%～90% in the cup, and cover rim of a cup with glass culture dish, after 30 days hardening, investigate its transplanting survival rate, potted plantly on the seedling of will taking root at last in the matrix of perlite+vermiculite+rural area soil (ratio 3: 1: 6), grow.
The invention has the beneficial effects as follows that having solved in the sea-buckthorn group training process sprouting starts slow, survival rate and the low problem of reproduction coefficient; adjustment by nutrient media components; induced sea-buckthorn to produce indefinite bud and higher rooting rate in a large number; transplant the back survival rate up to 80%; the quick breeding method for tissue culture of a kind of sea-buckthorn is provided, significant for the scale breeding of sea-buckthorn.
Embodiment
Choose full hippophae rhamnoides seed, remove the oil film of kind of outer cortex, be seeded in after the surface sterilization on the germination medium; The clip stem apex is set up the sterile test tube seedling as explant when being cultured to 28 days.The stem apex of clip test-tube plantlet 1.5～2.0cm is inoculated on the proliferated culture medium, statistics propagation multiple in the time of 50 days.Single seedling of clip robust growth, long 3～4cm is inoculated in root induction on the root media.Statistics rooting rate, mean elements and average root are long when being cultured to 50 days.When root continues to grow to 3～6cm, carry out acclimatization and transplants, after 30 days, investigate its transplanting survival rate.
Embodiment one:
Select full hippophae rhamnoides seed, be placed on after the surface sterilization on the germination medium and germinate; The germination medium comprises 6.5g/L agar powder and 30g/L white granulated sugar.After treating that true leaf grows fully, clip 1.5～2.0cm stem apex carries out enrichment culture, and minimal medium is WPM; Additional plant hormone is: the BA of 0.3mg/L; Also comprise 6.5g/L agar powder and 30g/L white granulated sugar.Single seedling of clip robust growth, long 3～4cm carries out culture of rootage, and minimal medium is 1/4SH; Additional plant hormone is: the IBA of 0.3mg/L and the NAA of 0.03mg/L; Also comprise 1.0g/LAC, 7.0g/L agar and 15g/L white granulated sugar, pH5.8; Cultivation temperature is that 25 ± 2 ℃, intensity of illumination are 1500～2000lx, and the photoperiod is 18h/d.Statistics propagation multiple, average plant height, rooting rate, mean elements and average root are long when being cultured to 50 days.The result shows that each explant average mark dissolves 3.19 indefinite buds, and average bud has reached 4.25cm; Rooting rate reaches 75.5%, and mean elements reaches 3.21, and average root reaches 0.65cm.Open hardening 3～6 days sealing film.Earlier press from both sides out seedling gently with tweezers, gently clean root medium in flowing water, soak 10min with 0.01% potassium permanganate again, transplant (perlite: vermiculite=3: 1) to the nutrient cup that matrix is housed, set upright seedling, shoot root is unfolded as far as possible, keep 20～25 ℃ of temperature, humidity 80%～90% in the cup, and cover rim of a cup with glass culture dish, through just there being new root to grow after 25 days the hardening, its survival rate can reach 70% in the time of 30 days, potted plantly on the seedling of will taking root at last grows in the matrix of perlite+vermiculite+rural area soil (ratio 3: 1: 6).
Embodiment two:
Select full hippophae rhamnoides seed, be placed on after the surface sterilization on the germination medium and germinate; The germination medium comprises 6.5g/L agar powder and 30g/L white granulated sugar.After treating that true leaf grows fully, clip 1.5～2cm stem apex carries out enrichment culture, and minimal medium is WPM; Additional plant hormone is: the BA of 0.4mg/L; Also comprise 6.5g/L agar powder and 30g/L white granulated sugar.Single seedling of clip robust growth, long 3～4cm carries out culture of rootage, and minimal medium is 1/4SH; Additional plant hormone is: the IBA of 0.4mg/L and the NAA of 0.04mg/L; Also comprise 1.0g/LAC, 7.0g/L agar and 15g/L white granulated sugar, pH5.8; Cultivation temperature is that 25 ± 2 ℃, intensity of illumination are 1500～2000lx, and the photoperiod is 18h/d.Statistics propagation multiple, average plant height, rooting rate, mean elements and average root are long when being cultured to 50 days.The result shows that each explant average mark dissolves 4.25 indefinite buds, and average bud has reached 5.42cm; Rooting rate reaches 78.8%, and mean elements reaches 3.85, and average root reaches 0.73cm.To seal film and open, hardening 3～6 days.Earlier press from both sides out seedling gently with tweezers, gently clean root medium in flowing water, soak 10min with 0.01% potassium permanganate again, transplant (perlite: vermiculite=3: 1) to the nutrient cup that matrix is housed, set upright seedling, shoot root is unfolded as far as possible, keep 20～25 ℃ of temperature, humidity 80%～90% in the cup, and cover rim of a cup with glass culture dish, just there is new root to grow through 23 days hardenings, survival rate can reach 73% in the time of 30 days, potted plantly on the seedling of will taking root at last grows in the matrix of perlite+vermiculite+rural area soil (ratio 3: 1: 6).
Embodiment three:
Select full hippophae rhamnoides seed, be placed on after the surface sterilization on the germination medium and germinate; The germination medium comprises 6.5g/L agar powder and 30g/L white granulated sugar.After treating that true leaf grows fully, clip 1.5～2cm stem apex carries out enrichment culture, and minimal medium is WPM; Additional plant hormone is: the BA of 0.5mg/L; Also comprise 6.5g/L agar powder and 30g/L white granulated sugar.Single seedling of clip robust growth, long 3～4cm carries out culture of rootage, and minimal medium is 1/4SH; Additional plant hormone is: the IBA of 0.5mg/L and the NAA of 0.05mg/L; Also comprise 1.0g/LAC, 7.0g/L agar and 15g/L white granulated sugar, pH5.8; Cultivation temperature is that 25 ± 2 ℃, intensity of illumination are 1500～2000lx, and the photoperiod is 18h/d.Statistics propagation multiple, average plant height, rooting rate, mean elements and average root are long when being cultured to 50 days.The result shows that each explant average mark dissolves 5.39 indefinite buds, and average bud has reached 6.48cm; Rooting rate reaches 81.7%, and mean elements reaches 4.25, and average root reaches 0.80cm.To seal film and open, hardening 3～6 days.Earlier press from both sides out seedling gently with tweezers, gently clean root medium in flowing water, soak 10min with 0.01% potassium permanganate again, transplant (perlite: vermiculite=3: 1) to the nutrient cup that matrix is housed, set upright seedling, shoot root is unfolded as far as possible, keep 20～25 ℃ of temperature, humidity 80%～90% in the cup, and cover rim of a cup with glass culture dish, just there is new root to grow through 20 days hardenings, survival rate can reach 80% in the time of 30 days, potted plantly on the seedling of will taking root at last grows in the matrix of perlite+vermiculite+rural area soil (ratio 3: 1: 6).
Embodiment four:
Select full hippophae rhamnoides seed, be placed on after the surface sterilization on the germination medium and germinate; The germination medium comprises 6.5g/L agar powder and 30g/L white granulated sugar.After treating that true leaf grows fully, clip 1.5～2cm stem apex carries out enrichment culture, and minimal medium is WPM; Additional plant hormone is: the BA of 0.6mg/L; Also comprise 6.5g/L agar powder and 30g/L white granulated sugar.Single seedling of clip robust growth, long 3～4cm carries out culture of rootage, and minimal medium is 1/4SH; Additional plant hormone is the IBA of 0.6mg/L and the NAA of 0.06mg/L; Also comprise 1.0g/L AC, 7.0g/L agar and 15g/L white granulated sugar, pH5.8; Cultivation temperature is that 25 ± 2 ℃, intensity of illumination are 1500～2000lx, and the photoperiod is 18h/d.Statistics propagation multiple, average plant height, rooting rate, mean elements and average root are long when being cultured to 50 days.The result shows that each explant average mark dissolves 5.10 indefinite buds, and average bud has reached 6.24cm; Rooting rate reaches 80.7%, and mean elements reaches 4.14, and average root reaches 0.79cm.To seal film and open, hardening 3～6 days.Earlier press from both sides out seedling gently with tweezers, gently clean root medium in flowing water, soak 10min with 0.01% potassium permanganate again, transplant (perlite: vermiculite=3: 1) to the nutrient cup that matrix is housed, set upright seedling, shoot root is unfolded as far as possible, keep 20～25 ℃ of temperature, humidity 80%～90% in the cup, and cover rim of a cup with glass culture dish, just there is new root to grow through 21 days hardenings, survival rate can reach 75% in the time of 30 days, potted plantly on the seedling of will taking root at last grows in the matrix of perlite+vermiculite+rural area soil (ratio 3: 1: 6).
The above results shows; the concentration of IBA and NAA in concentration by regulating BA in the proliferated culture medium and the root media; can significantly improve the propagation multiple of sea-buckthorn test-tube plantlet; and hestening rooting and improve its survival rate effectively; for the scale breeding of sea-buckthorn provides an effective way, significant.
Claims (1)
1. the tissue culture and rapid propagation method of a sea-buckthorn the steps include:
(1) selects full hippophae rhamnoides seed, be placed on after the surface sterilization on the germination medium and germinate; The germination medium comprises 6.5g/L agar powder and 30g/L white granulated sugar;
(2) treat that true leaf grows fully after, clip 1.5～2.0cm stem apex carries out enrichment culture, the minimal medium of enrichment culture is WPM medium: NH
4NO
3400mg/L, Ca (NO
3)
24H
2O 556mg/L, CaCl
22H
2O 96mg/L, MgSO
47H
2O 370mg/L, KH
2PO
4170mg/L, K
2SO
4990mg/L, H
3BO
36.2mg/L, MnSO
4H
2O 17.7mg/L, ZnSO
47H
2O 8.6mg/L, Na
2MoO
42H
2O 0.25mg/L, CuSO
45H
2O 0.25mg/L, FeSO
47H
2O 27.8mg/L, Na
2-EDTA 37.3mg/L, inositol 100mg/L, glycine 2mg/L, nicotinic acid 0.5mg/L, thiamine hydrochloride 1.0mg/L, puridoxine hydrochloride 0.5mg/L, 6.5g/L agar powder and 30g/L white granulated sugar; Additional plant hormone is the 6-benzylaminopurine of 0.3～0.6mg/L;
(3) single seedling of clip robust growth, long 3～4cm carries out culture of rootage, and the minimal medium of culture of rootage is 1/4SH medium: NH
4H
2PO
475mg/L, KNO
3625mg/L, CaCl
22H
2O 50mg/L, MgSO
47H
2O 100mg/L, KI 1mg/L, H
3BO
35.0mg/L, MnSO
4H
2O 7.6mg/L, ZnSO
47H
2O 1mg/L, Na
2MoO
42H
2O 0.1mg/L, CuSO
45H
2O 0.2mg/L, CoCl
26H
2O 0.1mg/L, FeSO
47H
2O15mg/L, Na
2-EDTA 20mg/L, inositol 1000mg/L, nicotinic acid 5.0mg/L, thiamine hydrochloride 5.0mg/L, puridoxine hydrochloride 0.5mg/L, 1.0g/L active carbon, 7.0g/L agar and 15g/L white granulated sugar; Additional plant hormone is the indolebutyric acid of 0.3～0.6mg/L, the methyl of 0.03～0.06mg/L; The culture of rootage temperature is that 25 ± 2 ℃, intensity of illumination are 1500～2000lx, and the photoperiod is 18h/d;
(4) when root continues to grow to 3～6cm, will seal film and open, hardening 3～6 days; Earlier press from both sides out seedling gently with tweezers, gently clean root medium in flowing water, soak 10min with 0.01% potassium permanganate again, transplanting to the perlite and the vermiculite that mixed with 3: 1 is in the nutrient cup of matrix, sets upright seedling, and shoot root is unfolded as far as possible, keep 20～25 ℃ of temperature, humidity 80%～90% in the cup, and cover rim of a cup with glass culture dish, after 30 days hardening, the seedling of will taking root is planted in the perlite, vermiculite and the rural area soil that mixed with 3: 1: 6 are the flowerpot of matrix and is grown.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2006100001940A CN100998314B (en) | 2006-01-09 | 2006-01-09 | Method for tissue culture quick breeding of sea-buckthorn |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2006100001940A CN100998314B (en) | 2006-01-09 | 2006-01-09 | Method for tissue culture quick breeding of sea-buckthorn |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN100998314A CN100998314A (en) | 2007-07-18 |
| CN100998314B true CN100998314B (en) | 2011-06-01 |
Family
ID=38257198
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2006100001940A Expired - Fee Related CN100998314B (en) | 2006-01-09 | 2006-01-09 | Method for tissue culture quick breeding of sea-buckthorn |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100998314B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103518775B (en) * | 2013-11-01 | 2016-02-24 | 北京林业大学 | A kind of Seabuckthorn growth regulator |
| CN107173226B (en) * | 2017-06-13 | 2019-09-24 | 新疆阿勒泰地区园林场 | A kind of sea-buckthorn tissue culture outside sprout-cultivating-bottle radication method |
| CN107041311A (en) * | 2017-06-13 | 2017-08-15 | 新疆阿勒泰地区园林场 | A kind of sea-buckthorn blade method for tissue culture |
| CN107155891A (en) * | 2017-06-13 | 2017-09-15 | 新疆阿勒泰地区园林场 | A kind of abundant kind big fruit sea-buckthorn stem tip tissue culture based formulas of the Liao Dynasty |
| CN108719056B (en) * | 2018-04-27 | 2021-07-16 | 新疆阿勒泰地区林业工作管理站 | Tissue culture seedling method of sea buckthorn |
| CN114503916B (en) * | 2022-03-03 | 2023-05-16 | 海南制药厂有限公司 | Artificial cultivation method of elaeagnus pungens |
-
2006
- 2006-01-09 CN CN2006100001940A patent/CN100998314B/en not_active Expired - Fee Related
Non-Patent Citations (6)
| Title |
|---|
| YAO Ying-mou er al..Micropropagation of sea buckthorn (Hippophae rhamnoidesL.).Agricultural Science in Finland4 5-6.1995,4(5-6),503-512. |
| YAO Ying-mou er al..Micropropagation of sea buckthorn (Hippophae rhamnoidesL.).Agricultural Science in Finland4 5-6.1995,4(5-6),503-512. * |
| 徐虹等.沙棘组织培养技术研究现状及存在问题.沙棘12 1.1999,12(1),11-13. |
| 徐虹等.沙棘组织培养技术研究现状及存在问题.沙棘12 1.1999,12(1),11-13. * |
| 李师翁等.大果良种沙棘愈伤组织诱导及植株再生的研究.西北植物学报21 2.2001,21(2),262-266. |
| 李师翁等.大果良种沙棘愈伤组织诱导及植株再生的研究.西北植物学报21 2.2001,21(2),262-266. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100998314A (en) | 2007-07-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103380730B (en) | Tissue-culture rapid propagation method for pyrus betulaefolia bunge | |
| JP2013150559A (en) | Method for cultivating fruit tree | |
| CN1989799A (en) | Sock growing nursery method for dwarf pear tree | |
| CN102845313A (en) | Method for quickly in-vitro actinidia kolomikta propagating | |
| CN100998314B (en) | Method for tissue culture quick breeding of sea-buckthorn | |
| CN102217550A (en) | Fast-propagation technology and composition of culture medium for virus-free plantlets of red bud taros | |
| CN108243944A (en) | A kind of iris rapid breeding method and fine quality tissue culture propagation | |
| KR100720338B1 (en) | Propagation Method of Japanese larchLarix leptolepis through Somatic Embryogenesis Technique | |
| CN103461143B (en) | Method for tissue culture and rapid propagation of camellia oleifera | |
| CN105918129B (en) | A kind of tissue culture and rapid propagation method of moonlight jujube | |
| Singh et al. | Identification of the suitable hardening protocol and hardening medium in micropropagation of gerbera (Gerbera jamesonii Bolus) | |
| CN110651715B (en) | Industrial seedling growing method for actinidia arguta | |
| CN108719057B (en) | Tissue culture rapid propagation and plant regeneration method for sea buckthorn | |
| CN1284444C (en) | Sterile seeding and tissue cultivating technology for Vanda | |
| CN101743908A (en) | Tissue culture, rapid propagation and cultivation method of grevillea banksii | |
| CN100391333C (en) | Aseptic seedling tissue culturing and test tube seedling hardening off and transplating technology for anthurium andraeanum | |
| CN102823495A (en) | Culture method for blueberries | |
| CN100998315B (en) | Direct sometic embryogenesis of sea-buckthorn, and regeneration of sea-buckthorn plants | |
| CN110558130B (en) | Cutting method of cauliflower | |
| CN108391591A (en) | A kind of Golden Bell Tree tissue cultivation rapid breeding method | |
| CN108112479A (en) | A kind of stem section of papaya sprout Bud Differentiation vacantly plants leaf promoting root growth method | |
| CN106258994A (en) | A kind of blue berry stem with bud induced bundle is sprouted regeneration method | |
| CN108719056B (en) | Tissue culture seedling method of sea buckthorn | |
| CN108617508B (en) | Rooting culture medium and tissue culture method for tissue culture of sea buckthorn | |
| JP4687028B2 (en) | Cutting plant seedling production method of Eucalyptus plant |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110601 Termination date: 20120109 |