CN104798670A - Non-woven fabric container cutting seedling raising method of Fraxinus velutina twigs - Google Patents
Non-woven fabric container cutting seedling raising method of Fraxinus velutina twigs Download PDFInfo
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- 238000005520 cutting process Methods 0.000 title claims abstract description 104
- 241000565362 Fraxinus velutina Species 0.000 title claims abstract description 34
- 238000000034 method Methods 0.000 title claims abstract description 32
- 239000004745 nonwoven fabric Substances 0.000 title abstract 4
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 42
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 20
- ONDPHDOFVYQSGI-UHFFFAOYSA-N zinc nitrate Chemical compound [Zn+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ONDPHDOFVYQSGI-UHFFFAOYSA-N 0.000 claims abstract description 20
- 238000005507 spraying Methods 0.000 claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 12
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims abstract description 11
- 239000004327 boric acid Substances 0.000 claims abstract description 11
- FYDKNKUEBJQCCN-UHFFFAOYSA-N lanthanum(3+);trinitrate Chemical compound [La+3].[O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O FYDKNKUEBJQCCN-UHFFFAOYSA-N 0.000 claims abstract description 10
- DBRMBYFUMAFZOB-UHFFFAOYSA-N molybdenum nitric acid Chemical compound [Mo].[N+](=O)(O)[O-] DBRMBYFUMAFZOB-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000002689 soil Substances 0.000 claims abstract description 9
- 241000609240 Ambelania acida Species 0.000 claims abstract description 8
- 239000010905 bagasse Substances 0.000 claims abstract description 8
- 235000019362 perlite Nutrition 0.000 claims abstract description 8
- 239000010451 perlite Substances 0.000 claims abstract description 8
- 239000002994 raw material Substances 0.000 claims abstract description 8
- 235000019354 vermiculite Nutrition 0.000 claims abstract description 8
- 239000010455 vermiculite Substances 0.000 claims abstract description 8
- 229910052902 vermiculite Inorganic materials 0.000 claims abstract description 8
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims abstract description 6
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims abstract description 6
- 239000006013 carbendazim Substances 0.000 claims abstract description 6
- 239000007921 spray Substances 0.000 claims description 50
- 239000000463 material Substances 0.000 claims description 22
- 230000008635 plant growth Effects 0.000 claims description 18
- 230000008569 process Effects 0.000 claims description 17
- 238000012136 culture method Methods 0.000 claims description 14
- 206010020649 Hyperkeratosis Diseases 0.000 claims description 12
- YGGXZTQSGNFKPJ-UHFFFAOYSA-N methyl 2-naphthalen-1-ylacetate Chemical compound C1=CC=C2C(CC(=O)OC)=CC=CC2=C1 YGGXZTQSGNFKPJ-UHFFFAOYSA-N 0.000 claims description 10
- 238000002360 preparation method Methods 0.000 claims description 10
- 238000004519 manufacturing process Methods 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- OVSKIKFHRZPJSS-UHFFFAOYSA-N 2,4-D Chemical compound OC(=O)COC1=CC=C(Cl)C=C1Cl OVSKIKFHRZPJSS-UHFFFAOYSA-N 0.000 claims description 5
- 230000015572 biosynthetic process Effects 0.000 claims description 5
- 150000001875 compounds Chemical class 0.000 claims description 5
- 239000012895 dilution Substances 0.000 claims description 5
- 238000010790 dilution Methods 0.000 claims description 5
- 239000011159 matrix material Substances 0.000 claims description 5
- 239000000243 solution Substances 0.000 claims description 5
- 239000002023 wood Substances 0.000 claims description 5
- 230000004083 survival effect Effects 0.000 abstract description 15
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 abstract 1
- OVSKIKFHRZPJSS-DOMIDYPGSA-N 2-(2,4-dichlorophenoxy)acetic acid Chemical compound OC(=O)[14CH2]OC1=CC=C(Cl)C=C1Cl OVSKIKFHRZPJSS-DOMIDYPGSA-N 0.000 abstract 1
- 239000000428 dust Substances 0.000 abstract 1
- 235000019441 ethanol Nutrition 0.000 abstract 1
- 239000002585 base Substances 0.000 description 13
- 239000005556 hormone Substances 0.000 description 7
- 229940088597 hormone Drugs 0.000 description 7
- VGKONPUVOVVNSU-UHFFFAOYSA-N naphthalen-1-yl acetate Chemical compound C1=CC=C2C(OC(=O)C)=CC=CC2=C1 VGKONPUVOVVNSU-UHFFFAOYSA-N 0.000 description 5
- 241001536358 Fraxinus Species 0.000 description 4
- 239000002253 acid Substances 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- 239000012174 chinese wax Substances 0.000 description 4
- 238000005286 illumination Methods 0.000 description 4
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 230000006698 induction Effects 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 241000565391 Fraxinus mandshurica Species 0.000 description 2
- 244000007021 Prunus avium Species 0.000 description 2
- 235000010401 Prunus avium Nutrition 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 102100040431 Activator of basal transcription 1 Human genes 0.000 description 1
- 244000072347 Fraxinus griffithii Species 0.000 description 1
- 101000964349 Homo sapiens Activator of basal transcription 1 Proteins 0.000 description 1
- 241000207834 Oleaceae Species 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000002520 cambial effect Effects 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000005213 imbibition Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000011122 softwood Substances 0.000 description 1
- 239000001993 wax Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a non-woven fabric container cutting seedling raising method of Fraxinus velutina twigs. The non-woven container cutting seedling method includes the following steps: (1) preparing cutting media: the cutting media are formed by preparing the following raw materials according to the volume percent: 15-25% of perlite, 5-15% of vermiculite, 65-75% of turfy soil, 2-7% of bagasse and 3-8% of saw dust, and spraying carbendazim solutions on the cutting media for disinfection; (2) preparing rooting agents: dissolving 100-400mg naphthylacetic acid in 40-50mL 95% ethyl alcohol, filling in 20-30mg 2,4-dichlorophenoxyacetic acid, 5-10mg zinc nitrate, 5-10mg nitric acid molybdenum, 5-10mg lanthanum nitrate and 40-50mg boric acid, filling in water until the solutions are at 1,000mL, and evenly mixing the solution; (3) preparing and treating cutting slips; (4) inserting the cutting slips into the cutting media, wherein the cutting depth is 8-9cm; (5) moving the non-woven fabric seedling raising container into a greenhouse, and erecting a shading screen for shading, wherein the shading percentage is 60-70%, the indoor temperature is kept at 26-29 DEG C, and the humidity is kept at 85-90%. The non-woven fabric container cutting seedling raising method of Fraxinus velutina twigs improves the survival rate and ensures to cultivate robust high-quality seedlings.
Description
Technical field
The present invention relates to a kind of Fraxinus velutina spray non-woven container cuttage and seedling culture method, belong to technical field of plant asexual propagation.
Background technology
Chinese wax is that Oleaceae (Oleceae) Fraxinus (Fraxinus) plant is referred to as, about 70 kinds, the whole world, about 34 kinds, China, wherein external introduction about 10 kinds.Ash tree kind is of a great variety, strong adaptability, material are good, purposes is wide, wherein Fraxinus velutina (F.velutina) growth is fast, the life-span is long, the precious broad-leaved material and reproducting tree species that a kind of economic worth is higher, and there is stronger salt stress-resistant ability, be also the important species of saline-alkali soil forestation.
Traditional Chinese wax breeding is in seminal propagation mode, seed seedling breaks up comparatively large and can not keep parent's good characteristic, and cottage propagation have simple, inexpensive efficient, the breeding cycle is shorter and keep the features such as elite stand good characteristic, can directly apply to actual production.Therefore cuttage seeding is more satisfactory afforestation material.China achieves certain progress to the research of the cottage propagations such as joint Chinese wax (Fraxinus Hulpehensis), evergreen Chinese wax (Fraxinus griffithii), Manchurian ash (F.mandshurica Rupr.) in recent years, but different researcher causes result of study to differ greatly due to method therefor difference, the conventional mode such as cuttage, press strip is adopted to breed, take root difficulty, reproduction coefficient is lower, can not meet the demand to a large amount of high quality seedling on producing.Zhang Jianguos etc. are with Fraxinus velutina--and No. 2, garden wax is experiment material, carries out Cutting test, and average cuttage survival rate reaches 87.1%, but this method is subject to a definite limitation not by any technological means on cuttage survival rate, Chinese patent application (CN102742495 B) discloses a kind of sweet cherry dwarfing rootstock epicormic branch cuttage non-woven container seedling-cultivating method and base composition, epicormic branch cuttage survival rate and seedling quality can be improved, but because sweet cherry is different from the characteristic of Fraxinus velutina seeds itself, and the Fraxinus velutina seeds that to be a kind of cottage propagation more difficult, seminal propagation or the grafting method of adopting is bred more at present, growing-seedling period is long, cost is high, constrain the propagation and employment of Fraxinus velutina improved seeds, the report being applied to Fraxinus velutina cutting propagation with the method for non-woven container nursery is there is not yet in prior art.
Summary of the invention
For the deficiency that above-mentioned prior art exists, the object of this invention is to provide a kind of Fraxinus velutina spray non-woven container cuttage and seedling culture method, by means of non-woven container seedling growing process, utilize not yet complete lignified spray to carry out cuttage and seedling culture under hot and humid environment, have that breeding is fast, cost is low, survival rate is high, be easy to advantages such as applying.
For achieving the above object, the present invention adopts following technical proposals:
A kind of Fraxinus velutina spray non-woven container cuttage and seedling culture method, comprises the following steps:
(1) cutting medium is prepared: cutting medium mixes formulated by the raw material of following percent by volume: perlite 15-25%, vermiculite 5-15%, turfy soil 65-75%, bagasse 2-7%, wood chaff 3-8%, utilizes dilution 800-1000 carbendazim solution doubly to spray cutting medium and carries out disinfection to it; Cutting medium after sterilization is put into container for plant growth made of nonwoven material, before cuttage, cutting medium is filled with permeable, the water content keeping cutting medium is 60-70%;
(2) root-growing agent is prepared: the consisting of of each component in root-growing agent: methyl α-naphthyl acetate 100-400mg/1000mL, 95% ethanol 40-50mL/1000mL, 2,4-dichlorphenoxyacetic acid 20-30mg/1000mL, zinc nitrate 5-10mg/1000mL, nitric acid molybdenum 5-10mg/1000mL, lanthanum nitrate 5-10mg/1000mL and boric acid 40-50mg/1000mL;
(3) preparation of cuttings and process: in mid or late June, semi-lignified spray raw is then selected to prepare cuttings, spray is cut into the cuttings of long 12 ~ 15cm, upper end otch is positioned at 1cm on blade inserted part, upper end otch is plane, inferior end notch is inclined-plane, each cuttings band 3-4 leaflet; Cuttings base portion 7-8cm is immersed in the root-growing agent that step (2) prepares, and soaks 0.5 ~ 2 hour;
(4) cuttage: in the cutting medium prepare the cuttings inserting step (1) after root-growing agent process, the cuttage degree of depth is 8-9cm;
(5) manage after cuttage: moved in greenhouse by the container for plant growth made of nonwoven material after cuttage, set up shading screen and carry out sunshade, shading rate is 60 ~ 70%; Keep the temperature in greenhouse to be 26 ~ 29 DEG C, humidity is 85 ~ 90%; In cuttage 15-20 days, carry out batch (-type) spraying, spraying and intermittent time set according to the change of intensity of illumination and temperature height, and spray 15 ~ 20 seconds/time in the morning of fine day, interval 6 minutes; Spray 20 seconds/time noon, interval 3 minutes; Spray 15 ~ 20 seconds/time afternoon, interval 5 minutes; Spray 15 ~ 20 seconds/time night, interval 15 minutes; Cloudy day spraying 10 ~ 15 seconds/time, interval 10 minutes; After cuttage 15-20 days, cuttings base portion forms callus, after 25-30 days, goes out coring, transplant after 50 days from the callus director of cuttings base portion.
In step (2), the root-growing agent compound method of every 1000mL is: be dissolved in by methyl α-naphthyl acetate 100-400mg in 95% ethanol of 40-50mL, then 2 are added, 4-dichlorphenoxyacetic acid 20-30mg, zinc nitrate 5-10mg, nitric acid molybdenum 5-10mg, lanthanum nitrate 5-10mg and boric acid 40-50mg, add water to 1000mL, mix, be namely mixed with root-growing agent.
Preferably, described cutting medium mixes formulated by the raw material of following percent by volume: perlite 15%, vermiculite 5%, turfy soil 70%, bagasse 4%, wooden chaff 6%.
Preferably, the consisting of of each component in described root-growing agent: methyl α-naphthyl acetate 200mg/1000mL, 95% ethanol 50mL/1000mL, 2,4-dichlorphenoxyacetic acid 25mg/1000mL, zinc nitrate 6mg/1000mL, nitric acid molybdenum 5mg/1000mL, lanthanum nitrate 5mg/1000mL and boric acid 50mg/1000mL.
The diameter of described container for plant growth made of nonwoven material is 5.8cm ~ 10cm, is highly 10cm, carries out filling production, container formation, matrix are filling, container cutting once completes by LKY type seedling forming machine.
The concentration of root-growing agent and the time of employing root-growing agent process cuttings have significant impact for the cuttage survival rate of Fraxinus velutina; The concentration of the root-growing agent of the present invention's preparation is 100-400PPM, belong to low concentration root-growing agent, the present invention's cuttings used is the spray of Fraxinus velutina, responsive to root-growing agent concentration-response, the method that traditional high concentration root-growing agent is quickly dipped is not also suitable for the cutting propagation of Fraxinus velutina of the present invention, because the cell division of the root-growing agent of high concentration to spray has certain inhibitory action, be unfavorable for calli induction.Prove through test of many times research, adopt low concentration root-growing agent of the present invention to soak 0.5-2 hour, fully can absorb root-growing agent, promote calli induction and take root, rooting rate reaches 100%.
Beneficial effect of the present invention:
(1) cutting medium of the present invention has good water imbibition and permeability, and organic content is high, significantly improves the cuttage survival rate of Fraxinus velutina.
(2) root-growing agent of the present invention is applied in the softwood cutting propagation nursery of Fraxinus velutina, effectively can activate cambial cell, makes otch form callus at short notice, rapid induction is taken root, meanwhile, owing to containing a certain amount of boric acid in root-growing agent, also there is the effect of sterilization and diseases prevention.
(3) in the cultivating process of Fraxinus velutina spray in non-woven container, root system propelling container wall also forms some sturdy callus, and form Balanced Root System through air root relief self-sow, root system closely can interweave with light ground mass and be integrated.Without seedling-slowing stage after field planting, primary growth amount (height of seedling, leading thread) also significantly improves, and greatly reduces cost of forestation simultaneously and significantly improves survival rate.
(4) cuttage and seedling culture operating process of the present invention is simple, not only reduces seedling cost, and significantly improves the quality of nursery stock, meets the active demand of production of forestry.
Embodiment
Below by instantiation, the present invention will be further elaborated, should be noted that following explanation is only to explain the present invention, not limiting its content.
Embodiment 1: Fraxinus velutina spray non-woven container cuttage and seedling culture method
Concrete steps are:
(1) cutting medium is prepared: sprayed by the carbendazim solution of cutting medium dilution 1000 times and it is carried out disinfection, wherein, cutting medium mixes formulated by the raw material of following percent by volume: perlite 15%, vermiculite 5%, turfy soil 70%, bagasse 4%, wood chaff 6%, it is 8cm that cutting medium after sterilization is put into diameter, be highly in the container for plant growth made of nonwoven material of 10cm, this container for plant growth made of nonwoven material carries out filling production by LKY type seedling forming machine, and container formation, matrix are filling, container cutting once completes.Be emitted on by container for plant growth made of nonwoven material in seedling growing box, before cuttage, cutting medium filled with permeable, the water content keeping cutting medium is 65%;
(2) root-growing agent is prepared: the root-growing agent compound method of every 1000mL is: be dissolved in 95% ethanol of 50mL by methyl α-naphthyl acetate (NAA) 200mg, then 2 are added, 4-dichlorphenoxyacetic acid 25mg, zinc nitrate 6mg, nitric acid molybdenum 5mg, lanthanum nitrate 5mg and boric acid 50mg, add water to 1000mL, mix, be namely mixed with root-growing agent;
(3) preparation of cuttings and process: in mid or late June, selects semi-lignified spray raw then, preparation cuttings, spray is cut into the cuttings of long 12 ~ 15cm, upper end otch is positioned at 1cm on blade inserted part, and upper end otch is plane, inferior end notch is inclined-plane, each cuttings band 4 leaflets; Cuttings base portion 7cm is immersed in the root-growing agent that step (2) prepares, and soaks 2 hours;
(4) cuttage: in the cutting medium prepare the cuttings inserting step (1) after root-growing agent process, the cuttage degree of depth is 8cm;
(5) manage after cuttage: moved in greenhouse by the container for plant growth made of nonwoven material after cuttage, set up shading screen and carry out sunshade, shading rate is 65%; Keep the temperature in greenhouse to be 28 DEG C, humidity is 90%.In cuttage 15 days, carry out batch (-type) spraying, spraying and intermittent time set according to the change of intensity of illumination and temperature height, and spray 18 seconds/time in the morning of fine day, interval 6 minutes; Spray 20 seconds/time noon, interval 3 minutes; Spray 16 seconds/time afternoon, interval 5 minutes; Spray 15 seconds/time night, interval 15 minutes; Cloudy day spraying 13 seconds/time, interval 10 minutes; Cuttage is after 15 days, and cuttings base portion forms callus, after 27 days, goes out coring from the callus director of cuttings base portion, starts to transplant after 50 days, and the survival rate after statistics transplanting, the 20 strain cuttings of the present embodiment, survive 20 strains after transplanting, survival rate is 100%.
Embodiment 2: Fraxinus velutina spray non-woven container cuttage and seedling culture method
Concrete steps are:
(1) cutting medium is prepared: sprayed by the carbendazim solution of cutting medium dilution 1000 times and it is carried out disinfection, wherein, cutting medium mixes formulated by the raw material of following percent by volume: perlite 25%, vermiculite 5%, turfy soil 65%, bagasse 2%, wood chaff 3%, it is 8cm that cutting medium after sterilization is put into diameter, be highly in the container for plant growth made of nonwoven material of 10cm, this container for plant growth made of nonwoven material carries out filling production by LKY type seedling forming machine, and container formation, matrix are filling, container cutting once completes.Be emitted on by container for plant growth made of nonwoven material in seedling growing box, before cuttage, cutting medium filled with permeable, the water content keeping cutting medium is 70%;
(2) root-growing agent is prepared: the root-growing agent compound method of every 1000mL is: be dissolved in 95% ethanol of 50mL by methyl α-naphthyl acetate (NAA) 100mg, then 2 are added, 4-dichlorphenoxyacetic acid 20mg, zinc nitrate 10mg, nitric acid molybdenum 5mg, lanthanum nitrate 10mg and boric acid 40mg, add water to 1000mL, mix, be namely mixed with root-growing agent;
(3) preparation of cuttings and process: in mid or late June, selects semi-lignified spray raw then, preparation cuttings, spray is cut into the cuttings of long 12 ~ 15cm, upper end otch is positioned at 1cm on blade inserted part, and upper end otch is plane, inferior end notch is inclined-plane, each cuttings band 4 leaflets; Cuttings base portion 7cm is immersed in the root-growing agent that step (2) prepares, and soaks 1 hour;
(4) cuttage: in the cutting medium prepare the cuttings inserting step (1) after root-growing agent process, the cuttage degree of depth is 8cm;
(5) manage after cuttage: moved in greenhouse by the container for plant growth made of nonwoven material after cuttage, set up shading screen and carry out sunshade, shading rate is 70%; Keep the temperature in greenhouse to be 26 DEG C, humidity is 85%.In cuttage 15 days, carry out batch (-type) spraying, spraying and intermittent time set according to the change of intensity of illumination and temperature height, and spray 18 seconds/time in the morning of fine day, interval 6 minutes; Spray 20 seconds/time noon, interval 3 minutes; Spray 16 seconds/time afternoon, interval 5 minutes; Spray 15 seconds/time night, interval 15 minutes; Cloudy day spraying 13 seconds/time, interval 10 minutes; Cuttage is after 20 days, and cuttings base portion forms callus, after 30 days, goes out coring from the callus director of cuttings base portion, starts to transplant after 50 days, and the survival rate after statistics transplanting, the 20 strain cuttings of the present embodiment, survive 19 strains after transplanting, survival rate is 95%.
Embodiment 3: Fraxinus velutina spray non-woven container cuttage and seedling culture method
Concrete steps are:
(1) cutting medium is prepared: sprayed by the carbendazim solution of cutting medium dilution 1000 times and it is carried out disinfection, wherein, cutting medium mixes formulated by the raw material of following percent by volume: perlite 20%, vermiculite 10%, turfy soil 65%, bagasse 2%, wood chaff 3%, it is 8cm that cutting medium after sterilization is put into diameter, be highly in the container for plant growth made of nonwoven material of 10cm, this container for plant growth made of nonwoven material carries out filling production by LKY type seedling forming machine, and container formation, matrix are filling, container cutting once completes.Be emitted on by container for plant growth made of nonwoven material in seedling growing box, before cuttage, cutting medium filled with permeable, the water content keeping cutting medium is 60%;
(2) root-growing agent is prepared: the root-growing agent compound method of every 1000mL is: be dissolved in 95% ethanol of 50mL by methyl α-naphthyl acetate (NAA) 400mg, then 2 are added, 4-dichlorphenoxyacetic acid 30mg, zinc nitrate 5mg, nitric acid molybdenum 5mg, lanthanum nitrate 5mg and boric acid 50mg, add water to 1000mL, mix, be namely mixed with root-growing agent;
(3) preparation of cuttings and process: in mid or late June, selects semi-lignified spray raw then, preparation cuttings, spray is cut into the cuttings of long 12 ~ 15cm, upper end otch is positioned at 1cm on blade inserted part, and upper end otch is plane, inferior end notch is inclined-plane, each cuttings band 4 leaflets; Cuttings base portion 7cm is immersed in the root-growing agent that step (2) prepares, and soaks 0.5 hour;
(4) cuttage: in the cutting medium prepare the cuttings inserting step (1) after root-growing agent process, the cuttage degree of depth is 8cm;
(5) manage after cuttage: moved in greenhouse by the container for plant growth made of nonwoven material after cuttage, set up shading screen and carry out sunshade, shading rate is 60%; Keep the temperature in greenhouse to be 29 DEG C, humidity is 90%.In cuttage 15 days, carry out batch (-type) spraying, spraying and intermittent time set according to the change of intensity of illumination and temperature height, and spray 18 seconds/time in the morning of fine day, interval 6 minutes; Spray 20 seconds/time noon, interval 3 minutes; Spray 16 seconds/time afternoon, interval 5 minutes; Spray 15 seconds/time night, interval 15 minutes; Cloudy day spraying 13 seconds/time, interval 10 minutes; Cuttage is after 15 days, and cuttings base portion forms callus, after 25 days, goes out coring from the callus director of cuttings base portion, starts to transplant after 50 days, and the survival rate after statistics transplanting, the 20 strain cuttings of the present embodiment, survive 18 strains after transplanting, survival rate is 90%.
Embodiment 4: different root-promoting hormone is to the results contrast of Fraxinus velutina cuttage and seedling culture
Investigate different root-promoting hormone (ABT1, IBA and NAA) results to Fraxinus velutina cuttage and seedling culture to compare, water is as blank; NAA in root-growing agent prescription in embodiment 1 is replaced to different root-promoting hormones, and adjusts its concentration and processing time, all the other operation with embodiment 1, the cuttage and seedling culture after different root-promoting hormone process the results are shown in Table 1.
The different root-promoting hormone of table 1 is to the result of Fraxinus velutina cuttage and seedling culture
As can be seen from Table 1, the impact of different root-promoting hormone process on the cuttage and seedling culture result of Fraxinus velutina differs greatly, and after adopting methyl α-naphthyl acetate of the present invention to process Fraxinus velutina as root-promoting hormone, its cuttage survival rate is more than 95%, and effect is better.
Claims (7)
1. a Fraxinus velutina spray non-woven container cuttage and seedling culture method, is characterized in that, comprise the following steps:
(1) cutting medium is prepared: cutting medium mixes formulated by the raw material of following percent by volume: perlite 15-25%, vermiculite 5-15%, turfy soil 65-75%, bagasse 2-7%, wood chaff 3-8%, utilizes dilution 800-1000 carbendazim solution doubly to spray cutting medium and carries out disinfection to it; Cutting medium after sterilization is put into container for plant growth made of nonwoven material, before cuttage, cutting medium is filled with permeable, the water content keeping cutting medium is 60-70%;
(2) root-growing agent is prepared, the consisting of of each component in root-growing agent: methyl α-naphthyl acetate 100-400mg/1000mL, 95% ethanol 40-50mL/1000mL, 2,4-dichlorphenoxyacetic acid 20-30mg/1000mL, zinc nitrate 5-10mg/1000mL, nitric acid molybdenum 5-10mg/1000mL, lanthanum nitrate 5-10mg/1000mL and boric acid 40-50mg/1000mL;
(3) preparation of cuttings and process: in mid or late June, selects semi-lignified spray raw then to prepare cuttings, and cuttings base portion 7-8cm is immersed in the root-growing agent that step (2) prepares, and soaks 0.5 ~ 2 hour;
(4) cuttage: in the cutting medium prepare the cuttings inserting step (1) after root-growing agent process, the cuttage degree of depth is 8-9cm;
(5) manage after cuttage: moved in greenhouse by the container for plant growth made of nonwoven material after cuttage, set up shading screen and carry out sunshade, shading rate is 60 ~ 70%; Keep the temperature in greenhouse to be 26 ~ 29 DEG C, humidity is 85 ~ 90%; In cuttage 15-20 days, carry out batch (-type) spraying, after cuttage 15-20 days, cuttings base portion forms callus, after 25-30 days, goes out coring, transplant after 50 days from the callus director of cuttings base portion.
2. a kind of Fraxinus velutina spray non-woven container cuttage and seedling culture method as claimed in claim 1, it is characterized in that, described cutting medium mixes formulated by the raw material of following percent by volume: perlite 15%, vermiculite 5%, turfy soil 70%, bagasse 4%, wooden chaff 6%.
3. a kind of Fraxinus velutina spray non-woven container cuttage and seedling culture method as claimed in claim 1, it is characterized in that, in step (2), the root-growing agent compound method of every 1000mL is: be dissolved in by methyl α-naphthyl acetate 100-400mg in 95% ethanol of 40-50mL, then add 2,4-dichlorphenoxyacetic acid 20-30mg, zinc nitrate 5-10mg, nitric acid molybdenum 5-10mg, lanthanum nitrate 5-10mg and boric acid 40-50mg, add water to 1000mL, mix, be namely mixed with root-growing agent.
4. a kind of Fraxinus velutina spray non-woven container cuttage and seedling culture method as claimed in claim 1, it is characterized in that, the consisting of of each component in described root-growing agent: methyl α-naphthyl acetate 200mg/1000mL, 95% ethanol 50mL/1000mL, 2,4-dichlorphenoxyacetic acid 25mg/1000mL, zinc nitrate 6mg/1000mL, nitric acid molybdenum 5mg/1000mL, lanthanum nitrate 5mg/1000mL and boric acid 50mg/1000mL.
5. a kind of Fraxinus velutina spray non-woven container cuttage and seedling culture method as claimed in claim 1, it is characterized in that, in step (3), the concrete preparation method of cuttings is: cuttings spray being cut into long 12 ~ 15cm, upper end otch is positioned at 1cm on blade inserted part, upper end otch is plane, and inferior end notch is inclined-plane, each cuttings band 3-4 leaflet.
6. a kind of Fraxinus velutina spray non-woven container cuttage and seedling culture method as claimed in claim 1, is characterized in that, in step (5), and being specifically set as of batch (-type) spraying: spray 15 ~ 20 seconds/time in the morning of fine day, interval 6 minutes; Spray 20 seconds/time noon, interval 3 minutes; Spray 15 ~ 20 seconds/time afternoon, interval 5 minutes; Spray 15 ~ 20 seconds/time night, interval 15 minutes; Cloudy day spraying 10 ~ 15 seconds/time, interval 10 minutes.
7. a kind of Fraxinus velutina spray non-woven container cuttage and seedling culture method as claimed in claim 1, it is characterized in that, the diameter of described container for plant growth made of nonwoven material is 5.8cm ~ 10cm, is highly 10cm, carry out filling production by LKY type seedling forming machine, container formation, matrix be filling, container cutting once complete.
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