CN103583360A - Method for improving Abelia seedling salt tolerance by oriented induction - Google Patents
Method for improving Abelia seedling salt tolerance by oriented induction Download PDFInfo
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Abstract
The invention discloses a method for improving Abelia seedling salt tolerance by oriented induction. The method comprises the steps of: (1) material sterilization, (2) induction of adventitious buds, (3) bud differentiation and subculture, (4) rooting culture, (5) seedling hardening, (6) seedling transplanting, and (7) seedling management. The method provided by the invention makes use of tissue culture and container seedling raising to add NaCl with different concentrations into a differentiation proliferation medium, a rooting medium and a culture matrix for transplanted seedlings gradually, so that the seedling salt tolerance can be enhanced from about 0.2% to about 0.6% (normal growth can be realized when the NaCl content of an annual seedling matrix is 0.4-0.5%, and normal growth can be realized when the NaCl content of a biennial seedling matrix is 0.5-0.6%), and the fixed planting survival rate in saline-alkali soil can be over doubled.
Description
Technical field
The present invention relates to ornamental trees and shrubs and cultivate technical field, specifically refer to utilize modern seedling growing process means to obtain the method that Abelia biflora nursery stock is improved salt tolerant alkalescence.
Background technology
Abelia biflora originates in China, is Caprifoliaceae Abelia plant, the foremost color leaf ornamental tree species of machaka ，Shi China.Abelia biflora has stronger adaptability, low temperature resistant, and resistance to soil is barren, has certain salt tolerant alkalescence and Drought resistance.Abelia biflora is one of important Landscape Trees of China's southeastern coast, but southeastern coast located in subtropical zone, annual rainfall is large, drenches salt desalinate gradually salinization soil by rainwater, and salt is grouped into take chloride as main, and salt content is 0.1%-0.8%.
Summary of the invention
Though the salt resistance that Abelia biflora tool is certain, inventor research team years of researches confirm, soil salt content is at 0.2% above Abelia biflora growth retardation, the 0.3%th, and the threshold values that its is grown.The methods such as cultivation are generally taked cuttage, grow directly from seeds and are organized in the breeding of Abelia biflora, test shows, if adopt cuttage and the breeding of growing directly from seeds, the salt content progressively increasing in seedling medium, also can improve the salt resistance of nursery stock, but cultivate and the means of Fast-propagation by tissue, the matrix of cultivating from tissue increases salt content gradually to the matrix of growing seedlings, the nursery stock salt resistance of producing is higher than cuttage and grow directly from seeds and breed the nursery stock obtaining.The present invention is based on the theory that plant is progressively tamed, the differential period of cultivating bud from tissue, to the nursery stock stage, is tamed the salt resistance that improves Abelia biflora by manual intervention.
Technical problem to be solved by this invention is the situation high for ground, existing China's southeastern coast tidal land seabeach salt content, Abelia biflora seedling planting survival rate is low, provides a kind of directional induction to improve the method for Abelia biflora nursery stock salt resistance.The present invention utilizes tissue to cultivate and container nursery means, at differentiation and increment medium, root media and the NaCl that progressively adds variable concentrations in the matrix that after transplanting seedlings, nursery stock is cultivated, the salt resistance that makes nursery stock is brought up to 0.6% left and right (one year seedling matrix, NaCl content 0.4-0.5% can normal growth, NaCl content 0.5-0.6% can normal growth in biennial seedling matrix) from 0.2% left and right, at the planting survival rates in saline land, be enhanced about more than once.
The present invention solves the problems of the technologies described above adopted technical scheme: the method comprises induction, differentiation and proliferation cultivation, the induction of root system and the cultivation of nursery stock etc. of Abelia biflora bud,
(1) material sterilizing: get Abelia biflora children shoot, be cut into 4-5cm stem segment with axillary bud, first soak 5-6 minute in cleaning solution, then water rinses the blade that cuts off expansion after 2-3 hour, be cut into the stem segment with axillary bud of 1.5cm; On superclean bench, with 75% alcoholic solution, soak 20 seconds, then proceed to mass fraction and be sterilizing 8-10 minute in 0.1% mercuric chloride solution, then use aseptic water washing 5-8 time;
(2) induction of indefinite bud: the stem section after described step (1) sterilizing is received on No. 1 medium and cultivated 40-50 days;
Wherein, on MS medium base, to add mass fraction be 0.1% NaCl to described No. 1 medium;
(3) differentiation of bud and subculture are cultivated: select the WD stem section of bud to receive on No. 2 medium, select well-grown bud subculture on same described No. 2 medium of transferring to cultivate once, be then transferred on No. 3 medium and cultivated for 2 generations; After several cultures, there is Multiple Buds;
Wherein, on MS medium base, to add mass fraction be 0.1% NaCl to described No. 2 medium; It is 0.2% NaCl that described No. 3 medium add mass fraction on MS medium base;
(4) culture of rootage: choose well-grown Multiple Buds and be divided into individual plant, go to root induction on No. 4 medium; Cultivate 15-20 days, root system is more than 5, and height of seedling 4-6cm can transplant seedlings; It is 0.3% NaCl that described No. 4 medium add mass fraction on the basis of 1/2MS medium;
(5) hardening: the bottleneck of well-grown seedling of taking root is opened, and at room temperature uncork hardening 2-3 days, takes out test-tube plantlet, rinses out after root medium with clear water, is transplanted on the husky bed that is provided with sea sand and carries out hardening; Described sea sand salt content 0.4%; During hardening, distance between rows and hills is 10 * 10cm; New root grows after extracting young leaves out and is transplanted in container and cultivates; Salt content in nurturing period chien shih sea sand is held constant at 0.4%;
(6) transplant seedlings: within 1 year, raw Abelia biflora container substrate composition is peat: sea sand=7:3 according to volume ratio, slow-release compound fertilizer 1kgm
-3; Within 2 years, raw Abelia biflora container substrate composition is peat: sea sand=6:4 according to volume ratio, slow-release compound fertilizer 2kg.m
-3; During preparation matrix, add NaCl, the NaCl content in one year seedling matrix is 0.5%, and the NaCl content in biennial seedling matrix is 0.6%;
(7) seedling management: container seedling is placed in beam-connected greenhouse, the temperature in beam-connected greenhouse remains on 20-25 ℃, and relative moisture of the soil remains on 65-75%.
In described step (2), described No. 1 medium also adds the kinetin of following material: 0.8-1.0mg/L, the sucrose that the indolebutyric acid of 0.08-0.12mg/L and mass fraction are 3%.
In described step (3), describedly contain that No. 2 medium also add the kinetin of following material: 0.4-0.6mg/L, the methyl α-naphthyl acetate of the 6-benzyl aminoadenine of 0.08-0.12mg/L, 0.05mg/L and sucrose that mass fraction is 3%.Wherein, mg/L represents the material milligram number containing in every liter of medium.
In described step (3), describedly contain that No. 3 medium also add the kinetin of following material: 0.4-0.6mg/L, the methyl α-naphthyl acetate of the 6-benzyl aminoadenine of 0.08-0.12mg/L, 0.04-0.06mg/L and sucrose that mass fraction is 3%.
In described step (4), described No. 4 medium also add the indolebutyric acid of following material: 0.4-0.6mg/L and the sucrose that mass fraction is 2%.
The condition of culture of described step (2), step (3) and step (4) is 24-25 ℃, relative air humidity 70-75%, light intensity 2500-3000Lx, illumination 10 hours/day.
In described step (5), transplant seedlings and within latter 10 days, take the interior sunshade net that is 50% with light transmittance.
In described step (5), described husky bed from bottom to top comprises bottom, intermediate layer and upper strata; Described bottom is the cobble of 8-15cm, the described intermediate layer earth that is 5cm, the sea sand that described upper strata is 20cm.
In described step (6), within 1 year, raw container for plant growth uses material mesh bag container, and diameter is 4-5cm, height 8-10cm; Within 2 years, raw container for plant growth uses material mesh bag container or plastic containers, and diameter is 18-20cm, is highly 300-310cm.
In described step (7), according to the upgrowth situation of nursery stock, within the 2nd year, change basin later, select material mesh bag container or plastic containers, diameter is 18-20cm, is highly 300-310cm, basin soil amount 800-1000g.
In described step (7), utilize the spraying facility of beam-connected greenhouse to spray.
In described step (7), during summer high temperature, container seedling seedling is carried out to sunshade, sunshade light transmittance is full exposure 60%.
During described step (5), step (6) and step (7), air themperature is more than or equal to 18 ℃, soil matrix temperature and is not less than 15 ℃.
Explanation of nouns
Cleaning solution: refer to that washing powder mass percentage concentration is at 0.4%～1% detergent solution, described washing powder can be can purchase the washing powder of available common brand on market, and described washing powder also can replace with neopelex.
MS medium: composition is as shown in table 1
The basic ingredient table of table 1:MS medium
1/2MS medium: refer on the basis of above-mentioned MS medium, the concentration of macroelement is reduced by half, and all the other compositions remain unchanged.
Salt content: " salt content " mentioned in the application refers to the mass percentage concentration of NaCl.
Content: " content " mentioned in the application refers to mass percentage concentration.
Material mesh bag container: the container that the poroid material of degradable fleece referring to is made.
Plastic containers: refer to the container that plastic material is made.
Removable plastic containers: be cultivating container common in nursery stock cultivation technique without soil, can obtain by business purchase approach.Its surrounding is cellular, and while growing seedlings, container two ends are fixed with draw-in groove, and it is cave-shaped that container is, and in the time of field planting, draw-in groove is removed, and container is flat condition.
Basin soil amount: the matrix weight containing in a container.
Advantage of the present invention and beneficial effect:
1. the present invention successfully adopts tissue to cultivate and container nursery means, progressively increases salt content and carry out salt resistance domestication in matrix, obtains its salt resistance of nursery stock and improves more than 60% than conventional seedbed system.The present invention obtains in the one year seedling matrix of Abelia biflora NaCl content 0.4%-0.5% can normal growth, NaCl content 0.5%-0.6% can normal growth in biennial seedling matrix.
The present invention by determine Abelia biflora bud induction, differentiation, subculture and culture of rootage and hardening, migrate to the matrix formulations in container, progressively increase the salt content in matrix, obtain a set of complete raising Abelia biflora Salt And Alkali Tolerance seedling-cultivating method.Key of the present invention be salt content in the configuration of each stage medium and medium control and each in stage plant screening.
3. although the present invention is by the means of progressively taming, obtain the high nursery stock of salt resistance, do not mean utilize this nursery stock for female parent carry out that follow-on Sterile culture (as cuttage, grafting and seminal propagation) obtained nursery stock there is higher salt resistance.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail, but the present invention is not only confined to following examples.
Embodiment 1 improves Abelia biflora " Edward " " (Abelia'Edward Goucher') be the method for salt resistance
1. materials disinfection: get the young shoot of Abelia biflora " Edward ", be cut into about 4-5cm stem segment with axillary bud, first in being added with the cleaning solution of washing powder (cleaning solution refers to the aqueous solution containing mass fraction 0.5% left and right neopelex), soak 5-6 minute, with running water flushing, after 2-3 hour, cut off the blade of expansion again, be cut into 1.5cm left and right stem with bud.On superclean bench, with 75% alcoholic solution, soak 20 seconds, then proceed to mass fraction and be sterilizing 8-10 minute in 0.1% mercuric chloride solution, and with aseptic water washing 5-8 time.
2. the induction of indefinite bud: the stem section of sterilizing is received on the medium of the NaCl that sucrose+mass fraction that MS+ kinetin 1.0mg/L+ indolebutyric acid 0.1mg/L+ mass fraction is 3% is 0.1% and cultivated 40-50 days.In the Induction Process of bud, constantly screen the good stem section of bud differential growth.
3. the differentiation of bud and subculture are cultivated: select the WD stem section of bud to receive on the proliferated culture medium of the NaCl that sucrose+mass fraction that MS+6-benzyl aminoadenine 0.1mg/L+ kinetin 0.5mg/L+ methyl α-naphthyl acetate 0.05mg/L+ mass fraction is 3% is 0.1%, select well-grown bud to transfer the upper subculture cultivation of same medium (NaCl that sucrose+mass fraction that MS+6-benzyl aminoadenine 0.1mg/L+ kinetin 0.5mg/L+ methyl α-naphthyl acetate 0.05mg/L+ mass fraction is 3% is 0.1%) once, then on the medium of the NaCl that to be transferred to sucrose+mass fraction that MS+6-benzyl aminoadenine 0.1mg/L+ kinetin 0.5mg/L+ methyl α-naphthyl acetate 0.05mg/L+ mass fraction is 3% be 0.2%, cultivated for 2 generations.After several cultures, there is Multiple Buds.
4. culture of rootage: choose well-grown Multiple Buds and be divided into individual plant, root induction on the NaCl root media that to go to sucrose+mass fraction that 1/2MS+ indolebutyric acid 0.5mg/L+ mass fraction is 2% be 0.3%.Cultivate about 15-20 days, root system is more than approximately 5, and height of seedling 4-6cm can transplant seedlings left and right.
More than group training chamber condition of culture is 25 ℃ of left and right, relative air humidity 70% left and right, light intensity 2500Lx left and right, illumination 10 hours/day.
5. hardening: eliminate the poor group training seedling of growth, the bottleneck of well-grown seedling of taking root is opened, at room temperature uncork hardening 2-3 days, takes out test-tube plantlet, rinses out after root medium with clear water, is transplanted on husky bed and carries out hardening.The cobble that husky bed bottom is 10cm left and right, middle earth, upper strata for 5cm left and right are the sea sand (sea sand salt content 0.4% left and right) of 20cm left and right.During hardening, distance between rows and hills is 10 * 10cm, according to soil and nursery stock blade face humidity, carries out aperiodically automatic irrigation, keeps the moistening not dehydration of nursery stock blade.After new root grows, progressively reduce the humidity of husky bed, after extracting young leaves out, be transplanted in container and cultivate.Notice that during this time the salt content in matrix keeps constant substantially, when salt content reduces in matrix, in sprinkling irrigation water, add appropriate NaCl.
6. transplant seedlings: transplant seedlings latter about 10 days with the interior sunshade net (light transmittance 50% left and right) of using.Within 1 year, raw container for plant growth material mesh bag container diameter is 4-5cm, height 8-10cm; 2 years raw container for plant growth material mesh bag containers and removable plastic containers diameter are 18-20cm, are highly 300-310cm.General 1 year raw chamber test matrix of Abelia biflora is peat: sea sand=7:3 according to volume ratio proportioning, slow-release compound fertilizer (1kgm-3); Within 2 years, raw container matrix is peat: sea sand=6:4 according to volume ratio proportioning, slow-release compound fertilizer (2kg.m-3).For prevention nursery stock generation damage by disease and insect, matrix palpus strict sterilization, adds NaCl during preparation matrix, and the NaCl content in one year seedling matrix is 0.4% left and right, and the NaCl content in biennial seedling matrix is 0.5% left and right.
7. seedling management: container seedling is placed in the beam-connected greenhouse of configuration sunshade and spraying facility, and the temperature in booth remains on 20-25 ℃, and relative moisture of the soil remains on 65-75%.According to the upgrowth situation of nursery stock, within the 2nd year, change basin, when biennial seedling matrix is changed basin, matrix is sea sand=6:4 according to volume ratio proportioning later, slow-release compound fertilizer (2kg.m-3); NaCl content in biennial seedling matrix is 0.5%.The container using can be material mesh bag container or removable plastic containers, and diameter is 18-20cm, is highly 300-310cm, basin soil amount 800-1000g.To being short of seedling or the poor container seedling that grows will be filled the gaps with seedlings in time.After filling the gaps with seedlings, to water immediately.In large-scale production, adopt and take sprinkling irrigation as good, can utilize beam-connected greenhouse spraying facility to spray.According to weather temp. and humidity in season, change and pour water, water and will irrigate, the Best Times that waters is morning or dusk.
Above hardening, transplant seedlings and nursery stage between air themperature be not less than 18 ℃, soil (matrix) temperature and be not less than 15 ℃.
In matrix, applied slow-release compound fertilizer, generally without refertilizing, but can be in conjunction with the suitable top dressing of pouring water according to container seedling growth situation.During summer high temperature, need container seedling seedling to carry out sunshade 60% left and right that sunshade light transmittance is full exposure.If any disease, occur, remove in time diseased plant, and use corresponding pesticide spraying sterilizing.As damaged by vermin occurs, control in time.Find that in container, matrix is sunk seedling stage, must add in time matrix, prevent that root is exposed.In seedling management, also want the salt content in nota bone matrix substantially to keep constant, when salt content reduces in matrix, in sprinkling irrigation water, add appropriate NaCl.
Facts have proved: soil salt content is at 0.2% above Abelia biflora " Edward " growth retardation, the 0.3%th, the threshold values of its growth.After the method for the present embodiment 1 is processed, in the one year seedling matrix of Abelia biflora " Edward ", in NaCl content 0.4% energy normal growth, biennial seedling matrix, NaCl content 0.5% can normal growth.
Embodiment 2 improves floral leaf Abelia biflora (formal name used at school: Abelia grandiflora " Francis the Mason ") method of salt resistance
1. materials disinfection: get floral leaf Abelia biflora children shoot, be cut into about 4-5cm stem segment with axillary bud, first in being added with the cleaning solution of washing powder (cleaning solution refers to the aqueous solution containing the neopelex of mass fraction 0.5%), soak 5-6 minute, with running water flushing, after 3 hours, cut off the blade of expansion again, be cut into the stem with bud of 1.5cm.On superclean bench, with 75% alcoholic solution, soak 20 seconds, then proceed to mass fraction and be sterilizing 8-10 minute in 0.1% mercuric chloride solution, and with aseptic water washing 6-8 time.
2. the induction of indefinite bud: the stem section of sterilizing is received on the medium of the NaCl that sucrose+mass fraction that MS+ kinetin 1.0mg/L+ indolebutyric acid 0.1mg/L+ mass fraction is 3% is 0.1% and cultivated 40-50 days.In the Induction Process of bud, constantly screen the good stem section of bud differential growth.
3. the differentiation of bud and subculture are cultivated: select the WD stem section of bud to receive on the proliferated culture medium of the NaCl that sucrose+mass fraction that MS+6-benzyl aminoadenine 0.1mg/L+ kinetin 0.5mg/L+ methyl α-naphthyl acetate 0.05mg/L+ mass fraction is 3% is 0.1%, select well-grown bud to transfer the upper subculture cultivation of same medium (proliferated culture medium of the NaCl that sucrose+mass fraction that MS+6-benzyl aminoadenine 0.1mg/L+ kinetin 0.5mg/L+ methyl α-naphthyl acetate 0.05mg/L+ mass fraction is 3% is 0.1%) once, then on the medium of the NaCl that to be transferred to sucrose+mass fraction that MS+6-benzyl aminoadenine 0.1mg/L+ kinetin 0.5mg/L+ methyl α-naphthyl acetate 0.05mg/L+ mass fraction is 3% be 0.2%, cultivated for 2 generations.After several cultures, there is Multiple Buds.
4. culture of rootage: choose well-grown Multiple Buds and be divided into individual plant, root induction on the NaCl root media that to go to sucrose+mass fraction that 1/2MS+ indolebutyric acid 0.5mg/L+ mass fraction is 2% be 0.3%.Cultivate about 15-20 days, root system is more than approximately 5, and height of seedling 4-6cm can transplant seedlings left and right.
More than group training chamber condition of culture is 25 ℃, relative air humidity 75%, light intensity 3000Lx, illumination 10 hours/day.
5. hardening: eliminate the poor group training seedling of growth, the bottleneck of well-grown seedling of taking root is opened, at room temperature uncork hardening 2-3 days, takes out test-tube plantlet, rinses out after root medium with clear water, is transplanted on husky bed and carries out hardening.The cobble that husky bed bottom is 10cm left and right, middle earth, upper strata for 5cm left and right are the sea sand (sea sand salt content 0.4% left and right) of 20cm left and right.During hardening, distance between rows and hills is 10 * 10cm, according to soil and nursery stock blade face humidity, carries out aperiodically automatic irrigation, keeps the moistening not dehydration of nursery stock blade.After new root extraction young leaves, be transplanted in container and cultivate.Notice that during this time the salt content in matrix keeps constant substantially, when salt content reduces in matrix, in sprinkling irrigation water, add appropriate NaCl.
6. transplant seedlings: transplant seedlings latter 10 days with the interior sunshade net (light transmittance 50% left and right) of using.Within 1 year, raw container for plant growth material mesh bag container diameter is 4-5cm, height 8-10cm; 2 years raw container for plant growth material mesh bag containers and removable plastic containers diameter are 18-20cm, are highly 300-310cm.1 year raw chamber test matrix of Abelia biflora is peat: sea sand=7:3 according to volume ratio proportioning, slow-release compound fertilizer (1kgm-3); Within 2 years, raw container matrix is peat: sea sand=6:4 according to volume ratio proportioning, slow-release compound fertilizer (2kg.m-3).For prevention nursery stock generation damage by disease and insect, matrix palpus strict sterilization, adds NaCl during preparation matrix, and the NaCl content in one year seedling matrix is 0.5%, and the NaCl content in biennial seedling matrix is 0.6%.
7. seedling management: container seedling is placed in the beam-connected greenhouse of configuration sunshade and spraying facility, and the temperature in booth remains on 20-25 ℃, and relative moisture of the soil remains on 65-75%.According to the upgrowth situation of nursery stock, within the 2nd year, change basin, when biennial seedling matrix is changed basin, matrix is sea sand=6:4 according to volume ratio proportioning later, slow-release compound fertilizer (2kg.m-3); NaCl content in biennial seedling matrix is 0.6%.The container using is material mesh bag container, and diameter is 18-20cm, is highly 300-310cm, basin soil amount 800-1000g.
Above hardening, transplant seedlings and nursery stage between air themperature be that 18 ℃, soil (matrix) temperature are 15 ℃.
In matrix, applied slow-release compound fertilizer, generally without refertilizing, but can be in conjunction with the suitable top dressing of pouring water according to container seedling growth situation.During summer high temperature, need container seedling seedling to carry out sunshade 60% left and right that sunshade light transmittance is full exposure.If any disease, occur, remove in time diseased plant, and use corresponding pesticide spraying sterilizing.As damaged by vermin occurs, control in time.Find that in container, matrix is sunk seedling stage, must add in time matrix, prevent that root is exposed.In seedling management, also want the salt content in nota bone matrix substantially to keep constant, when salt content reduces in matrix, in sprinkling irrigation water, add appropriate NaCl.
Facts have proved: soil salt content is at 0.2% above floral leaf Abelia biflora growth retardation, the 0.3%th, the threshold values of its growth.After the method for the present embodiment 1 is processed, in the one year seedling matrix of floral leaf Abelia biflora, in NaCl content 0.5% energy normal growth, biennial seedling matrix, NaCl content 0.6% can normal growth.
As mentioned above, just can realize preferably the present invention.
Claims (10)
1. directional induction improves a method for Abelia biflora nursery stock salt resistance, it is characterized in that: comprise the following steps:
(1) material sterilizing: get Abelia biflora children shoot, be cut into 4-5cm stem segment with axillary bud, first soak 5-6 minute in cleaning solution, then water rinses the blade that cuts off expansion after 2-3 hour, be cut into the stem segment with axillary bud of 1.5cm; On superclean bench, with 75% alcoholic solution, soak 20 seconds, then proceed to mass fraction and be sterilizing 8-10 minute in 0.1% mercuric chloride solution, then use aseptic water washing 5-8 time;
(2) induction of indefinite bud: the stem section after described step (1) sterilizing is received on No. 1 medium and cultivated 40-50 days;
Wherein, on MS medium base, to add mass fraction be 0.1% NaCl to described No. 1 medium;
(3) differentiation of bud and subculture are cultivated: select the WD stem section of bud to receive on No. 2 medium, select well-grown bud subculture on same described No. 2 medium of transferring to cultivate once, be then transferred on No. 3 medium and cultivated for 2 generations; After several cultures, there is Multiple Buds;
Wherein, on MS medium base, to add mass fraction be 0.1% NaCl to described No. 2 medium; It is 0.2% NaCl that described No. 3 medium add mass fraction on MS medium base;
(4) culture of rootage: choose well-grown Multiple Buds and be divided into individual plant, go to root induction on No. 4 medium; Cultivate 15-20 days, root system is more than 5, and height of seedling 4-6cm can transplant seedlings; It is 0.3% NaCl that described No. 4 medium add mass fraction on the basis of 1/2MS medium;
(5) hardening: the bottleneck of well-grown seedling of taking root is opened, and at room temperature uncork hardening 2-3 days, takes out test-tube plantlet, rinses out after root medium with clear water, is transplanted on the husky bed that is provided with sea sand and carries out hardening; Described sea sand salt content 0.4%; During hardening, distance between rows and hills is 10 * 10cm; New root grows after extracting young leaves out and is transplanted in container and cultivates; Salt content in nurturing period chien shih sea sand is held constant at 0.4%;
(6) transplant seedlings: within 1 year, raw Abelia biflora container substrate composition is peat: sea sand=7:3 according to volume ratio, slow-release compound fertilizer 1kgm
-3; Within 2 years, raw Abelia biflora container substrate composition is peat: sea sand=6:4 according to volume ratio, slow-release compound fertilizer 2kg.m
-3; During preparation matrix, add NaCl, the NaCl content in one year seedling matrix is 0.5%, and the NaCl content in biennial seedling matrix is 0.6%;
(7) seedling management: container seedling is placed in beam-connected greenhouse, the temperature in beam-connected greenhouse remains on 20-25 ℃, and relative moisture of the soil remains on 65-75%.
2. a kind of directional induction according to claim 1 improves the method for Abelia biflora nursery stock salt resistance, it is characterized in that: in described step (2), described No. 1 medium also adds the kinetin of following material: 0.8-1.0mg/L, the sucrose that the indolebutyric acid of 0.08-0.12mg/L and mass fraction are 3%;
In described step (3), describedly contain that No. 2 medium also add the kinetin of following material: 0.4-0.6mg/L, the methyl α-naphthyl acetate of the 6-benzyl aminoadenine of 0.08-0.12mg/L, 0.05mg/L and sucrose that mass fraction is 3%;
In described step (3), describedly contain that No. 3 medium also add the kinetin of following material: 0.4-0.6mg/L, the methyl α-naphthyl acetate of the 6-benzyl aminoadenine of 0.08-0.12mg/L, 0.04-0.06mg/L and sucrose that mass fraction is 3%;
In described step (4), described No. 4 medium also add the indolebutyric acid of following material: 0.4-0.6mg/L and the sucrose that mass fraction is 2%.
3. a kind of directional induction according to claim 1 improves the method for Abelia biflora nursery stock salt resistance, it is characterized in that: the condition of culture of described step (2), step (3) and step (4) is 24-25 ℃, relative air humidity 70-75%, light intensity 2500-3000Lx, illumination 10 hours/day.
4. a kind of directional induction according to claim 1 improves the method for Abelia biflora nursery stock salt resistance, it is characterized in that: in described step (5), transplant seedlings and within latter 10 days, take the interior sunshade net that is 50% with light transmittance.
5. a kind of directional induction according to claim 1 improves the method for Abelia biflora nursery stock salt resistance, it is characterized in that: in described step (5), described husky bed from bottom to top comprises bottom, intermediate layer and upper strata; Described bottom is the cobble of 8-15cm, the described intermediate layer earth that is 5cm, the sea sand that described upper strata is 20cm.
6. a kind of directional induction according to claim 1 improves the method for Abelia biflora nursery stock salt resistance, it is characterized in that: in described step (6), within 1 year, raw container for plant growth uses material mesh bag container, and diameter is 4-5cm, height 8-10cm; Within 2 years, raw container for plant growth uses material mesh bag container or plastic containers, and diameter is 18-20cm, is highly 300-310cm.
7. a kind of directional induction according to claim 1 improves the method for Abelia biflora nursery stock salt resistance, it is characterized in that: in described step (7), according to the upgrowth situation of nursery stock, within the 2nd year, change basin later, select material mesh bag container or plastic containers, diameter is 18-20cm, be highly 300-310cm, basin soil amount 800-1000g.
8. a kind of directional induction according to claim 1 improves the method for Abelia biflora nursery stock salt resistance, it is characterized in that: in described step (7), utilize the spraying facility of beam-connected greenhouse to spray.
9. a kind of directional induction according to claim 1 improves the method for Abelia biflora nursery stock salt resistance, it is characterized in that: in described step (7), during summer high temperature, container seedling seedling is carried out to sunshade, sunshade light transmittance is full exposure 60%.
10. a kind of directional induction according to claim 1 improves the method for Abelia biflora nursery stock salt resistance, it is characterized in that: during described step (5), step (6) and step (7), air themperature is more than or equal to 18 ℃, soil matrix temperature and is not less than 15 ℃.
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| CN107347645A (en) * | 2017-08-04 | 2017-11-17 | 上海市农业科学院 | A kind of method for tissue culture of great Hua Abelia bifloras |
| CN107347644A (en) * | 2017-08-04 | 2017-11-17 | 上海市农业科学院 | A kind of method that Regeneration in Vitro is carried out using great Hua Abelia bifloras blade |
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| CN105210756B (en) * | 2015-09-01 | 2019-05-21 | 福建省春天生态科技股份有限公司 | A kind of method for culturing seedlings improving extra large mango salt spray resistance ability |
| CN105594445A (en) * | 2016-02-25 | 2016-05-25 | 天津泰达盐碱地绿化研究中心有限公司 | Low-cost greening method for coastal hydraulic reclamation high-saline-and-alkaline exposed field |
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| CN107347644A (en) * | 2017-08-04 | 2017-11-17 | 上海市农业科学院 | A kind of method that Regeneration in Vitro is carried out using great Hua Abelia bifloras blade |
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