CN113439637A - Simplified seedling culture medium for date palm tillering seedlings and preparation method thereof - Google Patents
Simplified seedling culture medium for date palm tillering seedlings and preparation method thereof Download PDFInfo
- Publication number
- CN113439637A CN113439637A CN202110711966.6A CN202110711966A CN113439637A CN 113439637 A CN113439637 A CN 113439637A CN 202110711966 A CN202110711966 A CN 202110711966A CN 113439637 A CN113439637 A CN 113439637A
- Authority
- CN
- China
- Prior art keywords
- reagent
- seedling
- date palm
- tillering
- acid solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/25—Dry fruit hulls or husks, e.g. chaff or coir
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/30—Growth substrates; Culture media; Apparatus or methods therefor based on or containing synthetic organic compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N39/00—Biocides, pest repellants or attractants, or plant growth regulators containing aryloxy- or arylthio-aliphatic or cycloaliphatic compounds, containing the group or, e.g. phenoxyethylamine, phenylthio-acetonitrile, phenoxyacetone
- A01N39/02—Aryloxy-carboxylic acids; Derivatives thereof
- A01N39/04—Aryloxy-acetic acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/54—1,3-Diazines; Hydrogenated 1,3-diazines
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D5/00—Fertilisers containing magnesium
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/40—Mixtures of one or more fertilisers with additives not having a specially fertilising activity for affecting fertiliser dosage or release rate; for affecting solubility
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Wood Science & Technology (AREA)
- Agronomy & Crop Science (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Organic Chemistry (AREA)
- Botany (AREA)
- Soil Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Developmental Biology & Embryology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a simplified seedling raising substrate for date palm tillering seedlings and a preparation method thereof, and the simplified seedling raising substrate comprises a reagent A, a reagent B and a reagent C, wherein the reagent A is 1/4MS salt solution containing 800-fold diluted carbendazim and 800-fold diluted azoxystrobin; the reagent B is a mixed solution containing 300-600mg/L naphthylacetic acid solution, 100-200mg/L indolebutyric acid solution and 50-100 mg/L2, 4-dichlorophenoxyacetic acid solution; the volume ratio of the naphthylacetic acid solution to the indolebutyric acid solution to the 2, 4-dichlorophenoxyacetic acid solution is 2:1: 1.5; the reagent C is 0.3-0.7mg/L naphthylacetic acid solution. Also comprises a fixed matrix B and a rooting matrix C; wherein, the rooting substrate C comprises 65-75 percent of decomposed coconut coir, 7-15 percent of perlite, 5-10 percent of organic fertilizer, 7-15 percent of vermiculite and 0.1-0.4 percent of slow release fertilizer by volume percentage. The method adopts an external intervention method to carry out in-vitro culture on the date palm tillering seedlings, promotes rooting culture, and has the functions of resisting diseases and insects, promoting wound repair, accelerating rooting, ensuring sufficient nutrients, improving survival rate, being easy to ship and fix planting and the like.
Description
Technical Field
The invention relates to the technical field of plant biology, in particular to a simplified seedling culture substrate for date palm tillering seedlings and a preparation method thereof.
Background
The date palm tree (the scientific name: Phoenix dactylifera L.) belongs to the genus Abelmoschus in the family Palmaceae, is native to desert oasis in North Africa or peripheral areas of Persia bay in southwest Asia, is one of four fruit trees domesticated and cultivated by human beings at the earliest, and is planted in tropical and subtropical areas such as Hainan, Fujian, Guangxi, Yunnan, Guangdong and the like in China at present. The date palm fruit of date palm tree has rich nutritive value, sugar content as high as 55-70%, protein content as high as 3%, various amino acids, etc. and is also one of the main grains of Arabic.
The date palm belongs to male and female special-plant perennial crops, and the characters of seedlings are seriously separated, so that the excellent characters of female parents cannot be maintained. In order to ensure the planting yield and quality of the date palm trees in unit area, the conventional commercial cultivation is mainly carried out by cutting tillered seedlings of female plants for asexual propagation. At present, the international market has great demand on commercial seedlings of the date palm, but the date palm stock plant can only produce 15-30 mature tillering seedlings for a lifetime, and the tillering seedlings growing on the stock plant for 3-5 years can be taken down for in vitro propagation, so that the number of the tillering seedlings is limited, the culture survival rate is not high, and the rapid propagation of excellent seedlings is limited.
In foreign countries, two methods are generally adopted to cultivate tillering seedlings. One method is that tillering seedling is grown mature on the mother body, cut off directly and transferred to nursery garden for cultivation. The other method is to adopt different operations on the tillered seedlings growing at different parts of the parent body in order to promote the rooting, firstly, the tillered seedlings growing at the base part of the parent body are 12 months before being removed, and the base part of the tillered seedlings is covered with moist soil to promote the rooting; and the second is tillering seedling growing in the higher position of the parent trunk, and the root of the tillering seedling is fixed and moisturized by adopting a plastic bag or coarse linen filled with a matrix 12 months before the seedling is removed to promote rooting. Therefore, the existing tillering seedling cultivation technology has the following problems: 1. the tillering seedlings taken off from the parent bodies are easy to be infected by diseases and insect pests, and the survival rate is not high; 2. the seedling growth period of tillering seedling culture is long, and the seedling culture cost is high; 3. has no simple seedling raising matrix with specific root promoting, moisture keeping and fertilizer keeping functions.
Therefore, how to improve the survival rate of tillered seedling cultivation and increase the seedling speed under the condition of limited tillered seedling quantity is a problem which needs to be solved by the technical personnel in the field.
Disclosure of Invention
In view of the above, the invention aims to provide a simplified seedling culture substrate for date palm tillered seedlings and a preparation method thereof.
In order to achieve the purpose, the invention adopts the following technical scheme:
a simplified seedling culture medium for date palm tillered seedlings comprises a reagent A, a reagent B and a reagent C;
the reagent A is 1/4MS salt solution containing 800-fold diluted carbendazim and 800-fold diluted azoxystrobin; the reagent B is a mixed solution containing 300-600mg/L naphthylacetic acid solution, 100-200mg/L indolebutyric acid solution and 50-100 mg/L2, 4-dichlorophenoxyacetic acid solution; the volume ratio of the naphthylacetic acid solution to the indolebutyric acid solution to the 2, 4-dichlorophenoxyacetic acid solution is 2:1: 1.5; the reagent C is 0.3-0.7mg/L naphthylacetic acid solution.
Further, the date palm tillering seedling simplified seedling raising substrate also comprises a fixed substrate B and a rooting substrate C; the fixed substrate B is a substrate with the function of supporting the date palm tillering seedlings, preferably a mixture of vermiculite and quartz sand, and the volume ratio of the vermiculite to the quartz sand is (80-90): (10-20); the rooting substrate C comprises 65-75% of decomposed coconut coir, 7-15% of perlite, 5-10% of organic fertilizer, 7-15% of vermiculite and 0.1-0.4% of slow release fertilizer in percentage by volume.
The invention has the beneficial effects that:
in the reagent A, 1/4MS salt solution is used as a nutrition buffering agent for preventing the tillered seedling from losing nutrition supply instantly after separating from a parent body; the carbendazim is used as a broad-spectrum bactericide, has high efficiency and low toxicity, and has systemic treatment and protection effects; the azoxystrobin is mainly used for preventing and killing infectious diseases caused by fungi in the date palm. According to the invention, carbendazim and azoxystrobin are used in a matching manner, so that a synergistic effect is achieved in the treatment of the wound at the root of the tillering seedling, and the effects of preventing germ from infecting the root of the tillering seedling, accelerating the repair of the cut and improving the survival rate of the tillering seedling can be achieved.
In the reagent B, naphthylacetic acid (NAA) has the functions of inducing root system germination and promoting rooting; indolebutyric acid (IBA) and 2, 4-dichlorophenoxyacetic acid (2,4-D) are used as auxin substances, and have the effects of promoting wound repair and forming parenchyma cells. The invention carries out quick dipping treatment on three high-concentration hormones, namely naphthylacetic acid, indolebutyric acid and 2, 4-dichlorophenoxyacetic acid, stimulates wound to generate callus, and can achieve the effects of promoting quick rooting of tillered seedlings and shortening the seedling growth period of the tillered seedlings.
The decomposed coconut coir, perlite and vermiculite in the rooting substrate C are matched for use, so that an external environment for rapid root growth can be provided, tillering seedlings can uniformly absorb required nutrition, and the stable and healthy growth of the root system can be maintained under the long-term continuous action of low-concentration naphthylacetic acid. The organic fertilizer and the slow release fertilizer are used in a matching way to provide stable and continuous nutrition supply in the seedling stage. The simple root substrate has the effects of moisturizing, conserving fertilizer, accelerating the penetrability of the root system and achieving the effects of strengthening seedlings and facilitating shipment and transplantation.
Further, the reagent A is 1/4MS salt solution containing 1000 times diluted carbendazim and 800 times diluted azoxystrobin; the reagent B is a mixed solution containing 400mg/L of naphthylacetic acid solution, 150mg/L of indolebutyric acid solution and 80mg/L of 2, 4-dichlorophenoxyacetic acid solution; the reagent C is 0.5mg/L naphthylacetic acid solution.
Further, the rooting substrate C comprises 70% of decomposed coconut coir, 10% of perlite, 8% of organic fertilizer, 11.8% of vermiculite and 0.2% of slow release fertilizer in percentage by volume.
Furthermore, the fertilizer efficiency of the slow release fertilizer is (15-9-11+2MgO + TE) for more than 180 days.
Another object of the present invention is to provide a method for preparing the simplified seedling substrate for date palm tillering seedlings, which comprises the following steps:
(1) preparation of a reagent A: preparing 1/4MS salt solution by using sterile water, then weighing carbendazim and azoxystrobin with corresponding dilution times, and dissolving in 1/4MS salt solution to obtain a reagent A;
(2) preparation of reagent B: weighing naphthylacetic acid, indolebutyric acid and 2, 4-dichlorophenoxyacetic acid, respectively completely dissolving with sodium hydroxide solution, adjusting pH value to 5.5-6.0, adding sterile water to constant volume to corresponding concentration, and mixing according to the volume ratio of the naphthylacetic acid solution, the indolebutyric acid solution and the 2, 4-dichlorophenoxyacetic acid solution of 2:1:1.5 to obtain a reagent B;
(3) preparation of reagent C: weighing naphthylacetic acid, completely dissolving the naphthylacetic acid by using a sodium hydroxide solution, adjusting the pH value to 5.5-6.0, and adding sterile water to a constant volume to a corresponding concentration to obtain a reagent C.
Further, in the steps (2) and (3), the molar concentration of the sodium hydroxide solution is 1 mol/L.
Further, the preparation method of the simplified seedling culture substrate for the date palm tillered seedlings further comprises the following steps:
(4) preparing an immobilization substrate B: sterilizing the fixed matrix B, and soaking in sterile water for later use;
preferably, after the fixing matrix B is wrapped by kraft paper, high-temperature high-pressure moist heat sterilization is carried out;
(5) preparing a rooting substrate C: uniformly mixing the sterilized decomposed coconut coir, perlite, organic fertilizer, vermiculite and slow release fertilizer in proportion for later use.
Has the advantages that: the invention adopts sodium hydroxide solution to dissolve growth hormone such as naphthylacetic acid, and the like, and analyzes that naphthylacetic acid, indolebutyric acid, 2, 4-dichlorophenoxyacetic acid and the like in a pure state are powder, and are dissolved by using methanol or ethanol to prepare the solution with the required concentration, but the inventor finds that the naphthylacetic acid, indolebutyric acid, 2, 4-dichlorophenoxyacetic acid and the like dissolved by using methanol or ethanol are easy to precipitate after being placed, snowflake-shaped substances are precipitated in the solution, and the solution dissolved by using sodium hydroxide has stable properties and does not have the medicine precipitation phenomenon in the storage period.
The invention also aims to provide a use method of the coconut jujube tillering seedling simplified seedling raising substrate, which specifically comprises the following steps:
(1) pre-treating the date palm tillering seedlings;
(2) taking the date palm tillering seedling off the parent, soaking the base part of the date palm tillering seedling in the reagent A, taking out after 15-20min, and washing with sterile water to obtain a date palm tillering seedling I;
(3) soaking the base part of the date palm tillering seedling I in a reagent B, taking out after 40-50min, then planting in a fixed matrix B, planting one strain in each pot, spraying sterile water every day to keep the environment of the tillering seedling base part moist, and taking out after culturing for 30-40 days to obtain a date palm tillering seedling II;
(4) adding a reagent C into the rooting matrix C, wherein the volume ratio of the reagent C to the rooting matrix C is as follows: setting the ratio of the reagent C to 2:1, then fixing the value of a date palm tillering seedling II, fixedly planting one date palm tillering seedling in each pot, irrigating 1L of the reagent C every 25-35 days, continuously irrigating for 3-5 times, and spraying sterile water to keep the substrate C moist during the period to obtain the date palm tillering seedling with the fruiting root;
(5) and (5) strengthening seedlings and transplanting.
Further, in the step (1), the pre-treatment of the date palm tillering seedling comprises the following specific operations: before taking the date palm tillering seedling off, removing deadwood and base impurities of the date palm tillering seedling, spraying the whole date palm tillering seedling by using a mixture of 1500-fold diluent of 3% thiacloprid microcapsule suspending agent and 2000-fold diluent of 20% propiconazole missible oil and 1200-fold diluent of 1500-fold diluent of the date palm tillering seedling, spraying again after 14 days, and taking the date palm tillering seedling off from the mother body after 7 days.
Has the advantages that: before separating the tillered seedling from the parent body, the dead branches and the impurities at the base part of the tillered seedling are removed, the base part of the tillered seedling is exposed, and then the spraying treatment is carried out by using an insecticide and a bactericide, so that the worm eggs and germs carried by the tillered seedling are reduced, and the probability of the tillered seedling being infected by the germ can be reduced.
According to the technical scheme, compared with the prior art, the invention has the following beneficial effects:
1. according to the invention, carbendazim and azoxystrobin are used in a matching manner, so that a synergistic effect is achieved in the treatment of the wound at the root of the tillering seedling, and the effects of preventing germ from infecting the root of the tillering seedling, accelerating the repair of the cut and improving the survival rate of the tillering seedling can be achieved.
2. The invention carries out quick dipping treatment on three high-concentration hormones, namely naphthylacetic acid, indolebutyric acid and 2, 4-dichlorophenoxyacetic acid, and can achieve the effects of promoting quick rooting of tillered seedlings and shortening the seedling reviving period of the tillered seedlings.
3. The low-concentration hormone, the nutrition matrix and the rooting matrix act together, so that the robust growth of tillered seedlings can be ensured, and the development of root systems of the tillered seedlings can be promoted; the nutrient medium and the rooting medium have the advantages of good permeability, moisture retention, fertilizer retention and the like, the penetration of the root system and the absorption of nutrients are accelerated, and the strong seedling period is shortened; and the nutrition substrate and the rooting substrate are lighter in weight, so that the later-stage loading and transplanting of the tillering seedlings are facilitated, and the labor cost is saved.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The simplified seedling culture medium for the date palm tillered seedlings comprises a reagent A, a reagent B and a reagent C; wherein the reagent A is 1/4MS salt solution containing 800 times diluted carbendazim and 1200 times diluted azoxystrobin; the reagent B is a mixed solution containing 300mg/L of naphthylacetic acid solution, 200mg/L of indolebutyric acid solution and 100mg/L of 2, 4-dichlorophenoxyacetic acid solution; the volume ratio of the naphthylacetic acid solution to the indolebutyric acid solution to the 2, 4-dichlorophenoxyacetic acid solution is 2:1: 1.5; reagent C is 0.3mg/L naphthylacetic acid solution.
The date palm tillering seedling simplified seedling raising substrate also comprises a fixed substrate B and a rooting substrate C; wherein the fixed matrix B comprises 80 percent of vermiculite and 20 percent of quartz sand by volume percentage; the rooting substrate C comprises 65 percent of decomposed coconut coir, 15 percent of perlite, 5 percent of organic fertilizer, 14.9 percent of vermiculite and 0.1 percent of slow release fertilizer in percentage by volume. The slow release fertilizer comprises the components of (15-9-11+2MgO + TE).
The preparation method of the date palm tillering seedling raising substrate specifically comprises the following steps:
(1) preparation of a reagent A: preparing 1/4MS salt solution by using sterile water, then weighing carbendazim and azoxystrobin with corresponding dilution times, and dissolving in 1/4MS salt solution to obtain a reagent A;
(2) preparation of reagent B: weighing naphthylacetic acid, indolebutyric acid and 2, 4-dichlorophenoxyacetic acid, respectively and completely dissolving with 1mol/L sodium hydroxide solution, adjusting the pH value to 5.5-6.0, adding sterile water to a constant volume to a corresponding concentration, and mixing according to the volume ratio of the naphthylacetic acid solution, the indolebutyric acid solution and the 2, 4-dichlorophenoxyacetic acid solution of 2:1:1.5 to obtain a reagent B;
(3) preparation of reagent C: weighing naphthylacetic acid, completely dissolving the naphthylacetic acid by using 1mol/L sodium hydroxide solution, adjusting the pH value to 5.5-6.0, and adding sterile water to fix the volume to a corresponding concentration to obtain a reagent C.
(4) Preparing an immobilization substrate B: mixing vermiculite and quartz sand, wrapping with kraft paper, sterilizing at high temperature and high heat, taking out, and soaking with sterile water for use;
(5) preparing a rooting substrate C: uniformly mixing the sterilized decomposed coconut coir, perlite, organic fertilizer, vermiculite and slow release fertilizer in proportion for later use.
The application method of the date palm tillering seedling raising substrate specifically comprises the following steps:
(1) pretreating the date palm tillering seedlings, removing dead branches and base impurities of the date palm tillering seedlings, mixing 1500 times of diluent of 3% thiacloprid microcapsule suspending agent and 1500 times of diluent of 20% propiconazole missible oil to spray the whole date palm tillering seedlings, spraying again after 14 days, and taking the date palm tillering seedlings from a mother body after 7 days;
(2) taking the date palm tillering seedling off the parent, soaking the base part of the date palm tillering seedling in a reagent A, taking out after 15min, and washing with sterile water to obtain a date palm tillering seedling I;
(3) soaking the base part of the date palm tillering seedling I in a reagent B, taking out after 40min, then planting the date palm tillering seedling in a fixed matrix B, planting one strain in each pot, spraying sterile water to keep the environment moist, regularly checking the rooting condition of the wound, culturing for 30 days, and taking out to obtain a date palm tillering seedling II;
(4) adding a reagent C into the rooting matrix C, wherein the volume ratio of the reagent C to the rooting matrix C is as follows: setting the ratio of the reagent C to 2:1, then fixing the value of a date palm tillering seedling II, fixedly planting one date palm tillering seedling in each pot, irrigating 1L of the reagent C every 25 days, continuously irrigating for 5 times, and spraying sterile water to keep the substrate C moist during the period to obtain the date palm tillering seedling with the fruiting root;
(5) and (5) strengthening seedlings and transplanting.
Example 2
The simplified seedling culture medium for the date palm tillered seedlings comprises a reagent A, a reagent B and a reagent C; wherein the reagent A is 1/4MS salt solution containing 1000 times diluted carbendazim and 800 times diluted azoxystrobin; the reagent B is a mixed solution containing 400mg/L of naphthylacetic acid solution, 150mg/L of indolebutyric acid solution and 80mg/L of 2, 4-dichlorophenoxyacetic acid solution; the volume ratio of the naphthylacetic acid solution to the indolebutyric acid solution to the 2, 4-dichlorophenoxyacetic acid solution is 2:1: 1.5; reagent C is 0.5mg/L naphthylacetic acid solution.
The date palm tillering seedling simplified seedling raising substrate also comprises a fixed substrate B and a rooting substrate C; wherein the fixed matrix B comprises 85 percent of vermiculite and 15 percent of quartz sand by volume percentage; the rooting substrate C comprises 70% of decomposed coconut coir, 10% of perlite, 8% of organic fertilizer, 11.8% of vermiculite and 0.2% of slow release fertilizer in percentage by volume.
The preparation method of the date palm tillering seedling raising substrate specifically comprises the following steps:
(1) preparation of a reagent A: preparing 1/4MS salt solution by using sterile water, then weighing carbendazim and azoxystrobin with corresponding dilution times, and dissolving in 1/4MS salt solution to obtain a reagent A;
(2) preparation of reagent B: weighing naphthylacetic acid, indolebutyric acid and 2, 4-dichlorophenoxyacetic acid, respectively and completely dissolving with 1mol/L sodium hydroxide solution, adjusting the pH value to 5.5-6.0, adding sterile water to a constant volume to a corresponding concentration, and mixing according to the volume ratio of the naphthylacetic acid solution, the indolebutyric acid solution and the 2, 4-dichlorophenoxyacetic acid solution of 2:1:1.5 to obtain a reagent B;
(3) preparation of reagent C: weighing naphthylacetic acid, completely dissolving the naphthylacetic acid by using 1mol/L sodium hydroxide solution, adjusting the pH value to 5.5-6.0, and adding sterile water to fix the volume to a corresponding concentration to obtain a reagent C.
(4) Preparing an immobilization substrate B: mixing vermiculite and quartz sand, wrapping with kraft paper, sterilizing at high temperature and high heat, taking out, and soaking with sterile water for use;
(5) preparing a rooting substrate C: uniformly mixing the sterilized decomposed coconut coir, perlite, organic fertilizer, vermiculite and slow release fertilizer in proportion for later use.
The application method of the date palm tillering seedling raising substrate specifically comprises the following steps:
(1) pretreating the tillered seedlings of the date palms, removing dead branches and base impurities of the tillered seedlings of the date palms, mixing 1800 times of diluent of 3% thiacloprid microcapsule suspending agent and 1300 times of diluent of 20% propiconazole emulsifiable solution to spray the whole tillered seedlings of the date palms, spraying again after 14 days, and taking the tillered seedlings of the date palms from a mother body after 7 days;
(2) removing the date palm tillering seedling from the parent body, soaking the base part of the date palm tillering seedling in a reagent A, taking out after 18 times, and washing with sterile water to obtain a date palm tillering seedling I;
(3) soaking the base part of the date palm tillering seedling I in a reagent B, taking out after 45min, then planting the date palm tillering seedling in a fixed matrix B, planting one strain in each pot, spraying sterile water every day to keep the environment moist, regularly checking the rooting condition of the wound, culturing for 35 days, and taking out to obtain a date palm tillering seedling II;
(4) adding a reagent C into the rooting matrix C, wherein the volume ratio of the reagent C to the rooting matrix C is as follows: setting the ratio of the reagent C to 2:1, then setting the value of a date palm tillering seedling II, fixedly planting one date palm tillering seedling in each pot, quantitatively irrigating 1L of the reagent C every 30 days, continuously irrigating for 4 times, and spraying sterile water to keep the substrate C moist during the period to obtain the date palm tillering seedling with the fruiting root;
(5) and (5) strengthening seedlings and transplanting.
Example 3
The simplified seedling culture medium for the date palm tillered seedlings comprises a reagent A, a reagent B and a reagent C; wherein the reagent A is 1/4MS salt solution containing 1200 times diluted carbendazim and 1000 times diluted azoxystrobin; the reagent B is a mixed solution containing 600mg/L of naphthylacetic acid solution, 100mg/L of indolebutyric acid solution and 50mg/L of 2, 4-dichlorophenoxyacetic acid solution; the volume ratio of the naphthylacetic acid solution to the indolebutyric acid solution to the 2, 4-dichlorophenoxyacetic acid solution is 2:1: 1.5; reagent C is 0.7mg/L naphthylacetic acid solution.
The date palm tillering seedling simplified seedling raising substrate also comprises a fixed substrate B and a rooting substrate C; wherein the fixed matrix B comprises 90 percent of vermiculite and 10 percent of quartz sand by volume percentage; the rooting substrate C comprises 75% of decomposed coconut coir, 7% of perlite, 10% of organic fertilizer, 7.6% of vermiculite and 0.4% of slow release fertilizer in percentage by volume.
The preparation method of the date palm tillering seedling raising substrate specifically comprises the following steps:
(1) preparation of a reagent A: preparing 1/4MS salt solution by using sterile water, then weighing carbendazim and azoxystrobin with corresponding dilution times, and dissolving in 1/4MS salt solution to obtain a reagent A;
(2) preparation of reagent B: weighing naphthylacetic acid, indolebutyric acid and 2, 4-dichlorophenoxyacetic acid, respectively and completely dissolving with 1mol/L sodium hydroxide solution, adjusting the pH value to 5.5-6.0, adding sterile water to a constant volume to a corresponding concentration, and mixing according to the volume ratio of the naphthylacetic acid solution, the indolebutyric acid solution and the 2, 4-dichlorophenoxyacetic acid solution of 2:1:1.5 to obtain a reagent B;
(3) preparation of reagent C: weighing naphthylacetic acid, completely dissolving the naphthylacetic acid by using 1mol/L sodium hydroxide solution, adjusting the pH value to 5.5-6.0, and adding sterile water to fix the volume to a corresponding concentration to obtain a reagent C.
(4) Preparing an immobilization substrate B: mixing vermiculite and quartz sand, wrapping with kraft paper, sterilizing at high temperature and high heat, taking out, and soaking with sterile water for use;
(5) preparing a rooting substrate C: uniformly mixing the sterilized decomposed coconut coir, perlite, organic fertilizer, vermiculite and slow release fertilizer in proportion for later use.
The application method of the date palm tillering seedling raising substrate specifically comprises the following steps:
(1) pretreating the tillered seedlings of the date palms, removing dead branches and basic impurities of the tillered seedlings of the date palms, mixing 2000 times of diluent of 3% thiacloprid microcapsule suspending agent and 1200 times of diluent of 20% propiconazole emulsifiable concentrate to spray the whole tillered seedlings of the date palms, spraying again after 14 days, and taking the tillered seedlings of the date palms from a mother body after 7 days;
(2) removing the date palm tillering seedling from the parent body, soaking the base part of the date palm tillering seedling in a reagent A, taking out after 20min, and washing with sterile water to obtain a date palm tillering seedling I;
(3) soaking the base part of the date palm tillering seedling I in a reagent B, taking out after 50min, then planting the date palm tillering seedling in a fixed matrix B, planting one strain in each pot, spraying sterile water every day to keep the environment moist, regularly checking the rooting condition of the wound, culturing for 40 days, and taking out to obtain a date palm tillering seedling II;
(4) adding a reagent C into the rooting matrix C, wherein the volume ratio of the reagent C to the rooting matrix C is as follows: setting the ratio of the reagent C to 2:1, then fixing the value of a date palm tillering seedling II, fixedly planting one date palm tillering seedling in each pot, irrigating 1L of the reagent C every 35 days, continuously irrigating for 3 times, and spraying sterile water to keep the substrate C moist during the period to obtain the date palm tillering seedling with the fruiting root;
(5) and (5) strengthening seedlings and transplanting.
Comparative example 1
The date palm tillering seedling raising substrate is only different from the date palm tillering seedling raising substrate in the embodiment 2, and does not contain the reagent A.
Comparative example 2
The date palm tillering seedling raising substrate is only different from the date palm tillering seedling raising substrate in example 2 in that the date palm tillering seedling raising substrate does not contain a fixed substrate B and a reagent B.
Comparative example 3
The date palm tillering seedling raising substrate is different from the date palm tillering seedling raising substrate in the embodiment 2 only in that the date palm tillering seedling raising substrate does not contain a substrate C and a reagent C, and the date palm tillering seedling raising substrate is directly filled into a culture bag of sandy loam for raising seedlings after 1-3 steps in the using method of the date palm tillering seedling raising substrate in the embodiment 2 are completed.
Comparative example 4
The method is characterized in that the method does not carry out pretreatment, and the coconut jujube tillering seedlings are treated only by using a reagent A and then directly put into a culture bag made of sandy loam for seedling culture.
Performance testing
And counting the root number, the strong seedling period and the final survival rate of the date palm tillering seedlings in the examples 1 to 3 and the comparative examples 1 to 4 before the strong seedlings.
After the time from taking the tillering seedlings to processing and raising the seedlings reaches 150 days, the number of the primary roots and the number of the secondary roots are uniformly recorded, and after 365 days, the period of strong seedlings and the survival rate are counted, wherein the counting result is shown in table 1.
TABLE 1 statistical results of the seedlings grown in examples 1-3 and comparative examples 1-4
As can be seen from Table 1, compared with comparative example 4, the number of the primary roots and the secondary roots and the strong seedling period of the date palm tillering seedlings treated by the date palm tillering seedling raising substrates of comparative examples 1 to 3 are improved to a certain extent.
Compared with the comparative example 4, the number of the primary roots and the secondary roots and the strong seedling period of the date palm tillering seedlings treated by the date palm tillering seedling raising substrate in the examples 1 to 3 are remarkably improved and are obviously higher than those in the comparative examples 1 to 3. The method realizes the effects of improving the strong seedling period of the tillering seedlings of the date palm and accelerating the seedling raising speed.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (9)
1. A simplified seedling raising substrate for a date palm tillering seedling is characterized by comprising a reagent A, a reagent B and a reagent C;
the reagent A is 1/4MS salt solution containing 800-fold diluted carbendazim and 800-fold diluted azoxystrobin;
the reagent B is a mixed solution containing 300-600mg/L naphthylacetic acid solution, 100-200mg/L indolebutyric acid solution and 50-100 mg/L2, 4-dichlorophenoxyacetic acid solution;
the volume ratio of the naphthylacetic acid solution to the indolebutyric acid solution to the 2, 4-dichlorophenoxyacetic acid solution is 2:1: 1.5;
the reagent C is 0.3-0.7mg/L naphthylacetic acid solution.
2. The date palm tillering seedling simplified seedling raising substrate according to claim 1, characterized by further comprising a fixing substrate B and a rooting substrate C;
wherein, the rooting substrate C comprises 65-75 percent of decomposed coconut coir, 7-15 percent of perlite, 5-10 percent of organic fertilizer, 7-15 percent of vermiculite and 0.1-0.4 percent of slow release fertilizer by volume percentage.
3. The date palm tillering seedling simplification seedling raising substrate according to claim 1 or 2, characterized in that the reagent A is 1/4MS saline solution containing 1000 times diluted carbendazim and 800 times diluted azoxystrobin;
the reagent B is a mixed solution containing 400mg/L of naphthylacetic acid solution, 150mg/L of indolebutyric acid solution and 80mg/L of 2, 4-dichlorophenoxyacetic acid solution;
the reagent C is 0.5mg/L naphthylacetic acid solution.
4. The simplified seedling raising substrate for the date palm tillering seedlings according to claim 3, wherein the rooting substrate C comprises 70% of decomposed coconut coir, 10% of perlite, 8% of organic fertilizer, 11.8% of vermiculite and 0.2% of slow release fertilizer in percentage by volume.
5. A preparation method of the coconut jujube tillering seedling simplified seedling raising substrate as claimed in any one of claims 1 to 4, which is characterized by comprising the following steps:
(1) preparation of a reagent A: preparing 1/4MS salt solution by using sterile water, then weighing carbendazim and azoxystrobin with corresponding dilution times, and dissolving in 1/4MS salt solution to obtain a reagent A;
(2) preparation of reagent B: weighing naphthylacetic acid, indolebutyric acid and 2, 4-dichlorophenoxyacetic acid, respectively completely dissolving with sodium hydroxide solution, adding sterile water to a constant volume to reach corresponding concentration, and mixing according to the volume ratio of the naphthylacetic acid solution, the indolebutyric acid solution and the 2, 4-dichlorophenoxyacetic acid solution of 2:1:1.5 to obtain a reagent B;
(3) preparation of reagent C: weighing naphthylacetic acid, dissolving the naphthylacetic acid completely by using a sodium hydroxide solution, and adding sterile water to a constant volume to a corresponding concentration to obtain a reagent C.
6. The method for preparing a simplified coconut-date tillering seedling substrate according to claim 5, wherein the molar concentration of the sodium hydroxide solution in steps (2) and (3) is 1 mol/L.
7. The method for preparing a simplified coconut jujube tillering seedling substrate according to claim 5 or 6, further comprising:
(4) preparing an immobilization substrate B: sterilizing the fixed matrix B, and soaking in sterile water for later use;
(5) preparing a rooting substrate C: uniformly mixing the sterilized decomposed coconut coir, perlite, organic fertilizer, vermiculite and slow release fertilizer in proportion for later use.
8. A use method of the coconut jujube tillering seedling simplified seedling raising substrate as claimed in any one of claims 1 to 4, which is characterized by comprising the following steps:
(1) pre-treating the date palm tillering seedlings;
(2) taking the date palm tillering seedling off the parent, soaking the base part of the date palm tillering seedling in the reagent A, taking out after 15-20min, and washing with sterile water to obtain a date palm tillering seedling I;
(3) soaking the base part of the date palm tillering seedling I in a reagent B, taking out after 40-50min, then planting in a fixed matrix B, planting one strain in each pot, spraying sterile water every day to keep the environment of the tillering seedling base part moist, and taking out after culturing for 30-40 days to obtain a date palm tillering seedling II;
(4) adding a reagent C into the rooting matrix C, wherein the volume ratio of the reagent C to the rooting matrix C is as follows: setting the ratio of the reagent C to 2:1, then fixing the value of a date palm tillering seedling II, fixedly planting one date palm tillering seedling in each pot, irrigating 1L of the reagent C every 25-35 days, continuously irrigating for 3-5 times, and spraying sterile water to keep the substrate C moist during the period to obtain the date palm tillering seedling with the fruiting root;
(5) and (5) strengthening seedlings and transplanting.
9. The use method of the date palm tillering seedling simplified seedling culture medium according to the claim 8, characterized in that the pre-treatment of the date palm tillering seedling in the step (1) comprises the following specific operations: before taking the date palm tillering seedling off, removing deadwood and base impurities of the date palm tillering seedling, spraying the whole date palm tillering seedling by using a mixture of 1500-fold diluent of 3% thiacloprid microcapsule suspending agent and 2000-fold diluent of 20% propiconazole missible oil and 1200-fold diluent of 1500-fold diluent of the date palm tillering seedling, spraying again after 14 days, and taking the date palm tillering seedling off from the mother body after 7 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110711966.6A CN113439637B (en) | 2021-06-25 | 2021-06-25 | Light simplified seedling substrate for tillered seedlings of date palm and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110711966.6A CN113439637B (en) | 2021-06-25 | 2021-06-25 | Light simplified seedling substrate for tillered seedlings of date palm and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113439637A true CN113439637A (en) | 2021-09-28 |
| CN113439637B CN113439637B (en) | 2023-05-30 |
Family
ID=77812843
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110711966.6A Active CN113439637B (en) | 2021-06-25 | 2021-06-25 | Light simplified seedling substrate for tillered seedlings of date palm and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113439637B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115462290A (en) * | 2022-08-18 | 2022-12-13 | 昌江广凌农业科技有限公司 | Sweet potato seedling cultivation method capable of shortening growth cycle |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103392576A (en) * | 2013-08-14 | 2013-11-20 | 广西壮族自治区林业科学研究院 | Black wattle light medium container cutting seedling method |
| CN104798670A (en) * | 2015-04-30 | 2015-07-29 | 山东省林业科学研究院 | Non-woven fabric container cutting seedling raising method of Fraxinus velutina twigs |
| CN108781812A (en) * | 2018-06-22 | 2018-11-13 | 海南省农业科学院热带园艺研究所 | A kind of oil tea quickly rich root seedling cuttage and seedling culture method, seedling medium, root-growing agent |
| CN110810250A (en) * | 2019-12-16 | 2020-02-21 | 中国热带农业科学院椰子研究所 | Method for rapid propagation in tissue culture of date palm |
| CN110810243A (en) * | 2019-11-29 | 2020-02-21 | 中国热带农业科学院椰子研究所 | Callus induction culture method of date palm |
-
2021
- 2021-06-25 CN CN202110711966.6A patent/CN113439637B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103392576A (en) * | 2013-08-14 | 2013-11-20 | 广西壮族自治区林业科学研究院 | Black wattle light medium container cutting seedling method |
| CN104798670A (en) * | 2015-04-30 | 2015-07-29 | 山东省林业科学研究院 | Non-woven fabric container cutting seedling raising method of Fraxinus velutina twigs |
| CN108781812A (en) * | 2018-06-22 | 2018-11-13 | 海南省农业科学院热带园艺研究所 | A kind of oil tea quickly rich root seedling cuttage and seedling culture method, seedling medium, root-growing agent |
| CN110810243A (en) * | 2019-11-29 | 2020-02-21 | 中国热带农业科学院椰子研究所 | Callus induction culture method of date palm |
| CN110810250A (en) * | 2019-12-16 | 2020-02-21 | 中国热带农业科学院椰子研究所 | Method for rapid propagation in tissue culture of date palm |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115462290A (en) * | 2022-08-18 | 2022-12-13 | 昌江广凌农业科技有限公司 | Sweet potato seedling cultivation method capable of shortening growth cycle |
| CN115462290B (en) * | 2022-08-18 | 2023-09-08 | 昌江广凌农业科技有限公司 | Sweet potato seedling cultivation method capable of shortening growth period |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113439637B (en) | 2023-05-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108849984B (en) | Wheat drought-tolerance stress-tolerance compound regulator and application thereof | |
| CN111226704B (en) | Cutting seedling raising method for savatier shawense | |
| CN102144526A (en) | Detoxified seedling stem matrix floating seedling culturing method for potato | |
| CN105646081B (en) | Liquid compound fertilizer, preparation method and application thereof | |
| CN109258460A (en) | Micro-stem tip culture combines the breeding method of heat treatment acquisition Zengcheng honey chrysanthemum detoxic seedling | |
| CN105103930A (en) | Method for quickly cultivating murraya paniculata seedlings through cuttage | |
| CN105103931A (en) | Brucea-javanica female-plant-cuttage rapid seedling growing method | |
| CN113439637B (en) | Light simplified seedling substrate for tillered seedlings of date palm and preparation method thereof | |
| CN104542307A (en) | Culturing method of momordica cochinchinensis | |
| CN114532226A (en) | Culture medium combination and method for tissue culture and rapid propagation of curculigo orchioides | |
| CN112514797B (en) | Peach stock culture medium and use method thereof | |
| CN106605566A (en) | Pummelo peel seedling growing method | |
| CN111869569B (en) | Culture system for in vitro culture of hedychium japonicum flowers and application thereof | |
| CN111011381B (en) | Seed treatment agent for improving lodging resistance and yield of quinoa | |
| CN105981622A (en) | Populus euphratica twig cutting technique | |
| CN107873518B (en) | A kind of tissue culture method of Fourstamen Stephania Root seedling | |
| CN117481022A (en) | High-quality and high-yield substrate cultivation method for rhodiola crenulata | |
| CN108450332A (en) | A kind of Chinese toon stem section method for tissue culture | |
| CN103081805B (en) | Method for efficiently culturing tissues and industrially propagating robinia idaho | |
| CN106962093A (en) | A kind of breeding method of maple seedling | |
| CN114190276B (en) | Tissue culture and rapid propagation method for producing Panlongshen seedlings by using leaves | |
| CN110337981B (en) | Rapid seedling raising method for annona squamosa rootstocks | |
| CN114600757B (en) | Seedling raising method for hovenia dulcis thunb | |
| CN103947472B (en) | The garden landscape control method of ginkgo result | |
| CN111406521B (en) | Breeding method of Paeonia Macaca |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |