CN103081805B - Method for efficiently culturing tissues and industrially propagating robinia idaho - Google Patents
Method for efficiently culturing tissues and industrially propagating robinia idaho Download PDFInfo
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Abstract
The invention discloses a method for efficiently culturing tissues and industrially propagating robinia idaho. The method comprises the following steps: taking a branch of robinia idaho bron in those years, sterilizing, then inoculating a stem tip into an induction culture medium, culturing to obtain callus tissue block of the robinia idaho; cutting a callus tissue block into small blocks to inoculate to a callus tissue culture medium, culturing to obtain a callus tissue differentiated seedling of the robinia idaho; inoculating the differentiated seedling to a subculture medium, culturing to obtain subculture seedling of the robinia idaho; inoculating the subculture seedling into a rooting medium to culture to obtain a rooting seedling of the robinia idaho; transplanting a culture bottle with the rooting seedling to a greenhouse, firstly not decapping to domesticate, then decapping and domesticating; mixing turfy soil, vermiculite and fine sand with a certain proportion, spraying potassium permanganate and covering a plastic film for sterilizing, then taking the mixture as a planting substrate, filling the planting substrate in a nutrition bowl, planting the domesticated rooting seedling into the nutrition bowl, domesticating for a while, transplanting to the big field. According to the method, the added value of the tissue culture seedling by using the method disclosed by the invention achieves 15.2 times, the rate of survival of the nutrition bowl seedling transplanted to the greenhouse reaches more than 95%, and the production cost is reduced by 6.7%.
Description
Technical field
The present invention relates to Plant Tissue Breeding and expanding propagation technical field, be specifically related to a kind of method for tissue culture of fragrant flower Chinese scholar tree and the method for batch production expanding propagation.
Background technology
Fragrant flower Chinese scholar tree (Robinia pseudoacacia cv.idaho) has another name called rich and honour tree, belongs to Papilionaceae deciduous tree, originates in Spain.Fragrant flower Chinese scholar tree bark brown, smooth.Plant height 10-12 rice, leaf alternate, smooth, emerald green.Raceme armpit is raw, makes sagging shape, long 8-12 centimetre, and premium look, fragrance, in the north, annual May and July are opened flower twice, open every year flower in south 3-4 time.Florescence part is about 30 days, about 20 days Julies, about 15 days Augusts, about 10 days Septembers in may, has that flower is large, a feature such as Hua Xingmei, flower amount are many, florescence length.Spend sterilely, without pod, do not bear seeds.Fragrant flower Chinese scholar tree is cold-resistant, can resist subzero 25 degree to subzero 28 degree low temperature.Drought-resistant, barren-resistant, Salt And Alkali Tolerance, not tight to soil requirement, acid soil, neutral soil and light saline-alkali soil all can be grown.Root system is very flourishing, and rudiment is strong, and growth is fast.Fragrant flower Chinese scholar tree leaf body is thick, has stronger contamination resistance, and city poor environment is had to patience.Fragrant flower Chinese scholar tree is rare fragrant flower seeds, integrates greening, sweetening treatment, beautifies.Her pattern is gorgeous, and fragrance is strong, and flower amount is many, and the florescence is long, within 1 year, opens flower twice; Tree-like vigorous, attitude is graceful, can be widely used in gardens and trade greening; fragrant flower Chinese scholar tree is strong to the absorption of lead steam and adsorbing powder dust ability; function to environmental protection and air cleaning is remarkable, and fragrant flower Chinese scholar tree is described as 21 century eucalyptus, is the first-elected fast-growing ornamental tree species of afforestation.Utilize fragrant flower Chinese scholar tree drought resisting, cold-resistant, fast-growing, well developed root system feature, can be engaged in dust storm, arid area large area afforestation with other seeds, to checking winds and fixing drifting sand, improve the ecological environment and there is outstanding effect.Because fragrant flower Chinese scholar tree does not bear pods really, can be for breeding without seed, prior art is to breed by burying the asexual reproduction methods such as root propagation, branch cutting, propagation by grafiting and tissue culture propagation.Above-mentioned four kinds of asexual reproduction methods, no matter from reproduction speed, still breeding the method that cost is minimum is tissue culture method.
The report of the Study on tissue culture of relevant fragrant flower Chinese scholar tree is more, and the emphasis of research is the improvement to inducing culture, increment medium and root media, to improve reproduction coefficient and rooting rate.Because fragrant flower Chinese scholar tree is with a wide range of applications and fine development prospect, further carry out the research that fragrant flower Chinese scholar tree tissue is cultivated, improve its reproduction coefficient and survival rate and have great significance.
Summary of the invention
The object of the invention is for the deficiencies in the prior art; a kind of method that fragrant flower Chinese scholar tree high-efficiency tissue is cultivated and batch production is bred of scale is provided; application the method can make the successive propagation of fragrant flower Chinese scholar tree reach more than 15 times, and the transplanting survival rate of group training seedling from group training chamber to greenhouse reached more than 95%.
The object of the invention is to be achieved through the following technical solutions.
The method that fragrant flower Chinese scholar tree high-efficiency tissue is cultivated and batch production is bred, comprises the following steps:
(1) induction is cultivated
1) by MS+6-BA 0.6-0.8 mg/L+KT 0.02-0.05 mg/L+IAA 0.05-0.1 mg/L culture medium prescription, be mixed with inducing culture mother liquor;
2) get the agar of MS culture medium prescription quality 0.65%-0.75%, the coconut milk of 0.09%-0.10% mixes with medium mother liquor, medium is allocated as to PH6, in blake bottle, pack 20 ± 3 ml medium into, make solid inducing culture;
3) get fragrant flower Chinese scholar tree current-year branch, after sterilization, get the stem apex part of 0.5 mm size, be inoculated on inducing culture, 1 stem apex of every blake bottle inoculation;
4) blake bottle of having inoculated is put under the condition of intensity of illumination 1500Lx, 23-28 ℃, illumination every day 14h, cultivates 28 days, obtains fragrant flower Chinese scholar tree callus lines, standby.
(2) callus is cultivated
1) by MS+6-BA 0.3-0.5 mg/L+KT 0.05-0.08 mg/L+IAA 0.1-0.15 mg/L+NAA 0.08-0.1 mg/L+boric acid 6.8-7.0mg/L culture medium prescription, be mixed with callus medium mother liquor;
2) get the agar of MS culture medium prescription quality 0.65%-0.75%, the coconut milk of 0.09%-0.10% mixes with medium mother liquor, medium is allocated as to PH6, in blake bottle, pack 20 ± 3 ml medium into, make solid callus medium;
3) induction is cultivated to the fragrant flower Chinese scholar tree callus lines obtaining and under gnotobasis, be cut into the fritter of 0.4-0.6 cm, be inoculated on callus medium, every blake bottle inoculation 6-8 piece;
4) blake bottle of having inoculated is put under the condition of intensity of illumination 2500Lx, 23-28 ℃, illumination every day 14h, cultivates 25 days, obtains fragrant flower Chinese scholar tree Calli Differentiation seedling, standby.
(3) subculture is cultivated
1) press MS+6-BA 0.2-0.3 mg/L+KT 0.05-0.08 mg/L+IAA 0.05 mg/L+GA
31-1.2 mg/L+boric acid 6.8-7.0mg/L+sucrose 5 g/L culture medium prescriptions are mixed with subculture medium mother liquor;
2) get the agar of MS culture medium prescription quality 0.65%-0.75%, the coconut milk of 0.09%-0.10% mixes with medium mother liquor, medium is adjusted to PH6, in blake bottle, pack 20 ± 3 ml medium into, make solid subculture medium;
3) callus is cultivated to the Calli Differentiation seedling obtaining and by a bud one, saved segment under gnotobasis, be inoculated on subculture medium, every blake bottle inoculation 8-10 sections;
4) blake bottle of having inoculated is put under the condition of intensity of illumination 2500-3500Lx, 23-28 ℃, illumination every day 14h, cultivates 25 days, obtains fragrant flower Chinese scholar tree subculture and cultivates seedling, standby.
(4) culture of rootage
1) press MS+IBA 1.0-1.2 mg/L+NAA 0.01-0.02 mg/L+GA
310.2-10.3 mg/L+paclobutrazol 2.0-2.2 mg/L culture medium prescription is mixed with root media mother liquor;
2) get the agar of MS culture medium prescription quality 0.65%-0.75%, the coconut milk of 0.09%-0.10% mixes with medium mother liquor, medium is adjusted to PH6, in blake bottle, pack 20 ± 3 ml medium into, make solid root media;
3) subculture is cultivated to the cultivation seedling obtaining and by two buds one, saved segments under gnotobasis, be inoculated on root media, every blake bottle inoculation 9-10 sections;
4) blake bottle of having inoculated is put under the condition of intensity of illumination 2000-2500Lx, 23-28 ℃, illumination every day 14h, cultivates 15 days, obtains the fragrant flower Chinese scholar tree seedling of taking root, standby.
(5) tissue is cultivated the seedling domestication of taking root
1) blake bottle that tissue is housed cultivates the seedling of taking root is moved on in booth, not uncork domestication, domestication condition is: intensity of illumination 2000-4000lx, 18-30 ℃, domestication 72h;
2) blake bottle is opened to bottle cap domestication, domestication condition is: intensity of illumination 2000-4000lx, and 18-30 ℃, the relative moisture in blake bottle, more than 90%, is tamed 48h.
(6) tissue is cultivated the transplantation of seedlings of taking root
1), sieving after turfy soil insolation, by the ratio mixing of 4:1:1 by volume of turfy soil, vermiculite and fine sand, the potassium permanganate with 0.2% sprays to be mixed all, then with plastic foil, covering 24h carries out disinfection above, obtain the matrix of planting, the matrix of planting packs nutritive cube into, standby;
2) tissue of having tamed is cultivated to the seedling of taking root and takes out from blake bottle, wash medium off, plant into the nutritive cube that the matrix of planting is housed, accomplish the tip of a root down, upright, the shallow embedding of stem, gently press, floating;
3) nutritive cube of having planted seedling is emitted in booth, greenhouse-environment condition is controlled at 18-30 ℃, relative moisture 70%-90%, intensity of illumination 2000-10000Lx, start in 7 days that ventilation time is short, ventilation frequency wants high, after 7 days, strengthen gradually ventilation, tissue is cultivated the seedling of taking root and in booth, is tamed 23-25 days.
Beneficial effect of the present invention (1) adopts inducing culture of the present invention, and at intensity of illumination 1500Lx, under the condition of 23-28 ℃, illumination every day 14h induction is cultivated 28 days, then adopt callus medium of the present invention, at intensity of illumination 2500Lx, under the condition of 23-28 ℃, illumination every day 14h callus is cultivated 25 days, adopt again subculture medium of the present invention, at intensity of illumination 2500-3500Lx, under the condition of 23-28 ℃, illumination every day 14h subculture was cultivated after 25 days, the increment of group training seedling has reached 15.2 times, than 6 times of the group training seedling increments of prior art bibliographical information, 2.5 times have been improved, (2) adopt root media of the present invention, first at intensity of illumination 2000-2500Lx, under the condition of 23-28 ℃, illumination every day 14h, culture of rootage 15 days, average individual plant is taken root and is reached 3, then by condition of the present invention, tissue culture flasks seedling is tamed, finally tissue culture flasks transplantation of seedlings is arrived to turfy soil of the present invention, vermiculite, with the fine sand ratio of 4:1:1 by volume, sterile planting in matrix, according to tissue culture flasks transplantation of seedlings condition of the present invention, carry out standardized management, the survival rate that is transplanted to land for growing field crops nutrition pot seedling has reached more than 95%, compared with 90% of prior art report, 5 percentage points have been improved, (3) comparison of producing through more than the 500 ten thousand strain fragrant flower Chinese scholar tree seedlings of breeding more than 2 years, adopts method of the present invention tissue culture medium (TCM), condition of culture and domesticating method compared with before-improvement to reduce production costs 6.7%.
embodiment 1
(1) blake bottle is cleaned in the preparation of medium, use again purified rinse water, agar, medium are mixed, add water constant volume, get the agar of MS culture medium prescription quality 0.65%-0.75%, the coconut milk of 0.09%-0.10% mixes with medium mother liquor, medium is adjusted to PH6, each blake bottle packs 20 ± 3 ml into, then packs blake bottle into autoclave, sterilizing 20-22min under 121-122 ℃ of condition, after cooling, when taking the dish out of the pot, blake bottle carries out mark of correlation.The formula of the medium of various functions is respectively:
A inducing culture formula: MS+6-BA 0.6 mg/L+KT 0.05 mg/L+IAA 0.05 mg/L;
B callus culture medium prescription: MS+6-BA 0.5 mg/L+KT 0.05 mg/L+IAA 0.15 mg/L+NAA 0.08 mg/L+boric acid 6.8 mg/L;
C subculture medium formula: MS+6-BA 0.2 mg/L+KT 0.08 mg/L+IAA 0.05 mg/L+GA
31.2 mg/L+boric acid, 7.0 mg/L+sucrose, 5 g/L;
D prescription of rooting medium: MS+IBA 1.2 mg/L+NAA 0.01 mg/L+GA
310.3 mg/L+paclobutrazol, 2.0 mg/L.
(2) Shoot Tip Culture is got healthy and strong fragrant flower Chinese scholar tree current-year branch in May, cut off blade, running water rinses 10min; get full bud; successively with saturated washing powder water add that Tween 80 is scrubbed, running water is rinsed well, the alcohol with 75%, 0.1% mercuric chloride immersion 10min, aseptic water washing 5 times, put into sterile petri dish.Under aseptic condition, under anatomical lens, cut the stem apex part of 0.5 mm size, be inoculated on inducing culture 1 stem apex of every blake bottle inoculation.The blake bottle of having inoculated is put under the condition of intensity of illumination 1500Lx, 23-28 ℃, illumination every day 14h, cultivates 28 days, obtains fragrant flower Chinese scholar tree callus lines.
(3) callus is cultivated the callus lines of inducing cultivation to obtain is cut into the fritter of 0.4-0.6 cm under gnotobasis, be inoculated on callus medium, every blake bottle inoculation 6-8 piece, the blake bottle of having inoculated is put under the condition of intensity of illumination 2500Lx, 23-28 ℃, illumination every day 14h, cultivate 25 days, obtain fragrant flower Chinese scholar tree Calli Differentiation seedling.
(4) subculture is cultivated the Calli Differentiation seedling that callus cultivation is obtained and by a bud one, save segment under gnotobasis, be inoculated on subculture medium, every blake bottle inoculation 8-10 sections, the blake bottle of having inoculated is put under the condition of intensity of illumination 2500-3500Lx, 23-28 ℃, illumination every day 14h, cultivate 25 days, obtain fragrant flower Chinese scholar tree subculture and cultivate seedling.
(5) culture of rootage is cultivated by subculture the cultivation seedling obtaining and by two buds one, save segments under gnotobasis, be inoculated on root media, every blake bottle inoculation 9-10 sections, the blake bottle of having inoculated is put under the condition of intensity of illumination 2000-2500Lx, 23-28 ℃, illumination every day 14h, cultivate 15 days, obtain the fragrant flower Chinese scholar tree seedling of taking root.
(6) tissue is cultivated the seedling domestication of taking root and first the blake bottle that tissue is housed cultivates the seedling of taking root is moved on in booth, not uncork domestication, and domestication condition is: intensity of illumination 2000-4000lx, 18-30 ℃, tames 72h; Then blake bottle is opened to bottle cap domestication, domestication condition is: intensity of illumination 2000-4000lx, and 18-30 ℃, the relative moisture in blake bottle, more than 90%, is tamed 48h.
(7) tissue is cultivated and is taken root transplantation of seedlings sieving after turfy soil insolation, by the ratio mixing of 4:1:1 by volume of turfy soil, vermiculite and fine sand, potassium permanganate with 0.2% sprays to be mixed all, then with plastic foil, covering 24h carries out disinfection above, obtain the matrix of planting, the matrix of planting packs nutritive cube into; The tissue of having tamed is cultivated to the seedling of taking root and from blake bottle, takes out, wash medium off, plant into the nutritive cube that the matrix of planting is housed, accomplish the tip of a root down, upright, the shallow embedding of stem, gently press, floating; The nutritive cube of having planted seedling is emitted in booth, greenhouse-environment condition is controlled at 18-30 ℃, relative moisture 70%-90%, intensity of illumination 2000-10000Lx, start in 7 days that ventilation time is short, ventilation frequency wants high, after 7 days, strengthen gradually ventilation, tissue is cultivated the seedling of taking root and in booth, is tamed 23-25 days, survival rate reaches more than 95%, the land for growing field crops of then can planting.
embodiment 2
(1) preparation method who prepares medium of medium is with embodiment 1, the formula of the medium of various functions respectively:
A inducing culture formula: MS+6-BA 0.8 mg/L+KT 0.02 mg/L+IAA 0.1 mg/L;
B callus culture medium prescription: MS+6-BA 0.3 mg/L+KT 0.08 mg/L+IAA 0.1 mg/L+NAA 0.1 mg/L+boric acid 7.0mg/L;
C subculture medium formula: MS+6-BA 0.3 mg/L+KT 0.05 mg/L+IAA 0.05 mg/L+GA
31mg/L+boric acid 6.8 mg/L+ sucrose 5 g/L;
D prescription of rooting medium: MS+IBA 1.0 mg/L+NAA 0.02 mg/L+GA
310.2 mg/L+paclobutrazol, 2.2 mg/L.
(2) Shoot Tip Culture, callus cultivation, subculture cultivation, culture of rootage, the domestication of tissue culture flasks seedling, tissue culture flasks method for transplanting and condition are with embodiment 1.
Claims (1)
1. the method that fragrant flower Chinese scholar tree high-efficiency tissue is cultivated and batch production is bred, comprises the following steps:
(1) induction is cultivated
1) by MS+6-BA 0.6-0.8 mg/L+KT 0.02-0.05 mg/L+IAA 0.05-0.1 mg/L culture medium prescription, be mixed with inducing culture mother liquor;
2) get the agar of MS culture medium prescription quality 0.65%-0.75%, the coconut milk of 0.09%-0.10% mixes with medium mother liquor, medium is allocated as to pH6, in blake bottle, pack 20 ± 3 ml medium into, make solid inducing culture;
3) get fragrant flower Chinese scholar tree current-year branch, after sterilization, get the stem apex part of 0.5 mm size, be inoculated on inducing culture, 1 stem apex of every blake bottle inoculation;
4) blake bottle of having inoculated is put under the condition of intensity of illumination 1500Lx, 23-28 ℃, illumination every day 14h, cultivates 28 days, obtains fragrant flower Chinese scholar tree callus lines, standby;
(2) callus is cultivated
1) by MS+6-BA 0.3-0.5 mg/L+KT 0.05-0.08 mg/L+IAA 0.1-0.15 mg/L+NAA 0.08-0.1 mg/L+boric acid 6.8-7.0mg/L culture medium prescription, be mixed with callus medium mother liquor;
2) get the agar of MS culture medium prescription quality 0.65%-0.75%, the coconut milk of 0.09%-0.10% mixes with medium mother liquor, medium is allocated as to pH6, in blake bottle, pack 20 ± 3 ml medium into, make solid callus medium;
3) induction is cultivated to the fragrant flower Chinese scholar tree callus lines obtaining and under gnotobasis, be cut into the fritter of 0.4-0.6 cm, be inoculated on callus medium, every blake bottle inoculation 6-8 piece;
4) blake bottle of having inoculated is put under the condition of intensity of illumination 2500Lx, 23-28 ℃, illumination every day 14h, cultivates 25 days, obtains fragrant flower Chinese scholar tree Calli Differentiation seedling, standby;
(3) subculture is cultivated
1) press MS+6-BA 0.2-0.3 mg/L+KT 0.05-0.08 mg/L+IAA 0.05 mg/L+GA
31-1.2 mg/L+boric acid 6.8-7.0mg/L+sucrose 5 g/L culture medium prescriptions are mixed with subculture medium mother liquor;
2) get the agar of MS culture medium prescription quality 0.65%-0.75%, the coconut milk of 0.09%-0.10% mixes with medium mother liquor, medium is adjusted to pH6, in blake bottle, pack 20 ± 3 ml medium into, make solid subculture medium;
3) callus is cultivated to the Calli Differentiation seedling obtaining and by a bud one, saved segment under gnotobasis, be inoculated on subculture medium, every blake bottle inoculation 8-10 sections;
4) blake bottle of having inoculated is put under the condition of intensity of illumination 2500-3500Lx, 23-28 ℃, illumination every day 14h, cultivates 25 days, obtains fragrant flower Chinese scholar tree subculture and cultivates seedling, standby;
(4) culture of rootage
1) press MS+IBA 1.0-1.2 mg/L+NAA 0.01-0.02 mg/L+GA
310.2-10.3 mg/L+paclobutrazol 2.0-2.2 mg/L culture medium prescription is mixed with root media mother liquor;
2) get the agar of MS culture medium prescription quality 0.65%-0.75%, the coconut milk of 0.09%-0.10% mixes with medium mother liquor, medium is adjusted to pH6, in blake bottle, pack 20 ± 3 ml medium into, make solid root media;
3) subculture is cultivated to the cultivation seedling obtaining and by two buds one, saved segments under gnotobasis, be inoculated on root media, every blake bottle inoculation 9-10 sections;
4) blake bottle of having inoculated is put under the condition of intensity of illumination 2000-2500Lx, 23-28 ℃, illumination every day 14h, cultivates 15 days, obtains the fragrant flower Chinese scholar tree seedling of taking root, standby;
(5) tissue is cultivated the seedling domestication of taking root
1) blake bottle that tissue is housed cultivates the seedling of taking root is moved on in booth, not uncork domestication, domestication condition is: intensity of illumination 2000-4000lx, 18-30 ℃, domestication 72h;
2) blake bottle is opened to bottle cap domestication, domestication condition is: intensity of illumination 2000-4000lx, and 18-30 ℃, the relative moisture in blake bottle, more than 90%, is tamed 48h;
(6) tissue is cultivated the transplantation of seedlings of taking root
1), sieving after turfy soil insolation, by the ratio mixing of 4:1:1 by volume of turfy soil, vermiculite and fine sand, the potassium permanganate with 0.2% sprays to be mixed all, then with plastic foil, covering 24h carries out disinfection above, obtain the matrix of planting, the matrix of planting packs nutritive cube into, standby;
2) tissue of having tamed is cultivated to the seedling of taking root and takes out from blake bottle, wash medium off, plant into the nutritive cube that the matrix of planting is housed, accomplish the tip of a root down, upright, the shallow embedding of stem, gently press, floating;
3) nutritive cube of having planted seedling is emitted in booth, greenhouse-environment condition is controlled at 18-30 ℃, relative moisture 70%-90%, intensity of illumination 2000-10000Lx, start in 7 days that ventilation time is short, ventilation frequency wants high, after 7 days, strengthen gradually ventilation, tissue is cultivated the seedling of taking root and in booth, is tamed 23-25 days.
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| CN109548656A (en) * | 2019-01-15 | 2019-04-02 | 北京蓉坤农业科技有限公司 | A kind of fragrant flower Chinese scholar tree method for tissue culture |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1582641A (en) * | 2004-05-25 | 2005-02-23 | 山东省林业科学研究院 | Tetraploid transgenic locust and quick cultivation |
| CN101803524A (en) * | 2010-04-26 | 2010-08-18 | 北京林业大学 | Hardwood cutting propagation method for tetraploid robinia pseudoacacia |
| CN102499087A (en) * | 2011-11-01 | 2012-06-20 | 北京林业大学 | Isolated culture and plant regeneration method of silver chain |
| CN102499086A (en) * | 2011-11-01 | 2012-06-20 | 北京林业大学 | Method for breeding locust |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| USPP9771P (en) * | 1995-04-25 | 1996-12-31 | Trustees Of The Peter Cunningham Family Trust | Robinia plant named `Lace Lady` |
-
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1582641A (en) * | 2004-05-25 | 2005-02-23 | 山东省林业科学研究院 | Tetraploid transgenic locust and quick cultivation |
| CN101803524A (en) * | 2010-04-26 | 2010-08-18 | 北京林业大学 | Hardwood cutting propagation method for tetraploid robinia pseudoacacia |
| CN102499087A (en) * | 2011-11-01 | 2012-06-20 | 北京林业大学 | Isolated culture and plant regeneration method of silver chain |
| CN102499086A (en) * | 2011-11-01 | 2012-06-20 | 北京林业大学 | Method for breeding locust |
Non-Patent Citations (4)
| Title |
|---|
| 王玲仙.香花槐器官的组织培养及植株再生研究.《北方园艺》.2008,(第1期),第191-193页. |
| 王秋竹.香花槐的组织培养研究.《吉林农业科技学院学报》.2008,第17卷(第3期),第12-15页. |
| 香花槐器官的组织培养及植株再生研究;王玲仙;《北方园艺》;20081231(第1期);全文 * |
| 香花槐的组织培养研究;王秋竹;《吉林农业科技学院学报》;20080930;第17卷(第3期);全文 * |
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