CN102742495A - Method for utilizing non-woven fabric container to perform sweet cherry dwarfing rootstock twig cuttage seedling culture and matrix combination - Google Patents
Method for utilizing non-woven fabric container to perform sweet cherry dwarfing rootstock twig cuttage seedling culture and matrix combination Download PDFInfo
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Abstract
The invention discloses a method for utilizing a non-woven fabric container to perform sweet cherry dwarfing rootstock twig cuttage seedling culture and a matrix combination. The method mainly comprises light matrix preparation, manufacture of fertilizer in seedling culture matrix and the non-woven fabric seedling culture container, central sand filling and placing of the non-woven fabric seedling culture container, cutting slips manufacture and cuttage, preparation and application of a microorganism preparation (cherry rhizosphere growth-promoting bacterium YT3 with preservation number to be CGMCCNo.5319) and management after cuttage. The matrix combination is composed of a matrix main body material, the fertilizer and the microorganism preparation. The method improves survival rate of twig cuttage and seedling quality, shortens seedling culture time, reduces seedling culture cost, improves seedling culture efficiency of sweet cherry dwarfing rootstock, and meets the requirements of massive high-quality seedlings for sweet cherry production.
Description
Technical field
The present invention relates to a kind of sweet cherry dwarfing rootstock epicormic branch cuttage nonwoven container seedling culture method and base composition.
Background technology
Sweet cherry be favored by the consumer, high-grade fruit that market prospects are wide; Supply falls short of demand in market, holds at high price, and development potentiality is huge; Utilizing dwarfing rootstock to carry out planting culture, is to realize sweet cherry early reality, high yield, high-quality, efficient, safe important measures.But the sweet cherry dwarfing rootstock is a clonal rootstock, can not adopt seminal propagation.Adopt modes such as conventional cuttage, press strip to breed, the difficulty of taking root, reproduction coefficient are low, can not satisfy the demand that goes up a large amount of high quality seedlings of producing; The employing tissue culture mode is bred, and specification requirement is high, the breeding cycle is long, breed the cost height, has restricted applying of sweet cherry dwarfing rootstock.
Sweet cherry dwarfing rootstock epicormic branch cuttage is the asexual reproduction method that utilizes incomplete as yet lignified young sprout to carry out cuttage, has to breed fast, low, the technological wield characteristics of cost, is suitable for producing scale and uses.In recent years, both at home and abroad the technical staff at stock selection, cutting medium, cutting time, insert aspects such as ear selection is selected, root-growing agent processing and done a large amount of research work.One Chinese patent application CN 101433171 B disclose a kind of sweet cherry dwarfing rootstock in-vitro tender tip container Cheng Miaofa; The rooting rate and the new talent transplanting survival rate of the sweet cherry dwarfing rootstock in-vitro tender tip are significantly improved; But this method is had relatively high expectations to cutting, and seedling raise period is longer.One Chinese patent application CN 102138486 A disclose a kind of method of cherry test-tube plantlet cuttage and quick-propagation, have shortened the breeding cycle of nursery stock, reduce and breed cost, but the specification requirement that the cherry test-tube plantlet is bred is higher.
Therefore, further promote sweet cherry dwarfing rootstock epicormic branch cuttage technology, the survival rate of raising epicormic branch cuttage and seedling quality, shortening seedling raise period, reduction seedling cost have become the active demand of present production.
Summary of the invention
The objective of the invention is for overcoming the deficiency of above-mentioned prior art; A kind of microorganism formulation and nonwoven container epicormic branch cuttage sweet cherry dwarfing rootstock technology utilized is provided; Thereby the survival rate of raising epicormic branch cuttage and seedling quality, shortening seedling raise period, reduction seedling cost; Promote the benefit of growing seedlings of sweet cherry dwarfing rootstock, satisfy the demand of sweet cherry production a large amount of high quality seedlings.
The forest-science academy, Shandong Province has formed the light matrix nonwoven container nursery technology that tallies with the national condition on the basis of introducing French Fertiss seedling growing process; This seedling raising manners has solved the ubiquitous nest root of conventional vessel seedling, problems such as root, rare, weak root partially, traditional seedling raising manners of substituted for plastic membrane container filling nutrition soil progressively.
The short endophytic bacteria of plant rhizosphere can be secreted the plant growth-promoting material, comprises plant hormone, vitamin, amino acid and other active organic molecule and derivative etc.; Also can improve the nutrient environment of plant rhizosphere, effective controlling plant disease etc.
For realizing above-mentioned purpose, the present invention adopts following technical proposals:
A kind of base composition that is used for sweet cherry dwarfing rootstock epicormic branch cutting is characterized in that it comprises:
(1) matrix body material: the matrix body material is made up of the raw material of following weight portion, peat: 40-60 part, perlite: 40-60 part;
(2) fertilizer: fertilizer is S in the weight of matrix body material, and consumption is distinguished as follows: the urea of 0.05-0.1%S weight, the potassium chloride of the superphosphate of 0.3-0.5%S weight, 0.03-0.05%S weight;
(3) microorganism formulation: (deposit number is CGMCC No.5319 with the short living bacterium YT3 of cherry rhizosphere; Be 201110376284.0 at application number; Name is called in the patent of " a strain cherry rhizosphere short living pseudomonad and application " thereof to be announced); After the activated cultivation, be diluted to 1.0 * 108cfu/mL.
The addition of microorganism is every slotting fringe of 10-20mL/ in this composition.
A kind of sweet cherry dwarfing rootstock epicormic branch cuttage nonwoven container seedling culture method, concrete steps are following:
(1) matrix for preparing is filled in the nonwoven container for plant growth, will inserts fringe afterwards and insert in the matrix;
(2) be the short living bacterium YT3 of cherry rhizosphere of CGMCC No.5319 with deposit number, obtain suspension after the activation culture, get suspension and evenly water around slotting fringe;
(3) the laggard administration-management reason of cuttage is grown seedlings.
Said matrix comprises matrix body material and fertilizer; Wherein the matrix body material is made up of the raw material of following weight portion; Peat: 40-60 part, perlite: 40-60 part, fertilizer is S in the weight of matrix body material; Consumption is distinguished as follows: the urea of 0.05-0.1%S weight, the potassium chloride of the superphosphate of 0.3-0.5%S weight, 0.03-0.05%S weight.
It also comprises the process to the matrix sterilization, promptly in matrix body material and fertilizer, adds the carbendazim of 0.08-0.12%S weight, and is blended together, fully mixes thoroughly.
After being filled to matrix in the nonwoven container for plant growth, in the matrix of container center, punching, and in the hole, fill river sand, will insert then in the river sand of fringe insertion container center.
Said nonwoven container for plant growth is cylindric, and diameter is 5.8cm, highly is 10cm that the rifle bar of employing diameter 1-2cm is beaten the hole of dark 3-4cm in the matrix of container center, in the hole, fill river sand then; In the time of in the river sand of slotting fringe insertion container center, the cuttage degree of depth is 3-4cm.
Said slotting fringe processing method is following: in 6～August, select semi-lignified branch living then, that growth is enriched to carry out cuttage, choose the middle and upper part of branch, rugosity is advisable at 0.3～0.5cm; Cutting is cut into the slotting fringe of growing 10～12cm, and radical leaves removes, and keeps a pair of blade in top, and blade is cut half, with soaking 1.5～3.5 hours in the ABT rooting powder solution of slotting fringe base portion with 150～300ppm that shears.
With the deposit number of preserving is the short living bacterium YT3 of cherry rhizosphere of CGMCC No.5319; Activation on the LB inclined-plane, picking one articulating is gone in the beef-protein medium, in 37 ℃; Shaken cultivation is 2 days under the rotating speed 180rpm condition; Utilize colony counting method to measure every milliliter of bacteria containing amount, calculate bacterial concentration, be diluted to 1.0 * 10 then
8Cfu/mL; After cuttage 10-15 days, get the 10-20mL suspension and evenly water around cherry is inserted fringe.
The method that the laggard administration-management reason of said cuttage is grown seedlings is: the summer high temperature phase need be set up shady net and carried out sunshade, and shady net light transmittance is preferably 50% of full exposure; With intermittent automated spray system control moisture, in the cuttage 15～20 days, keep the blade face not do after the cuttage; After the cuttage 15 days, the new root of transplanting a cutting begins to take shape, control moisture, and the assurance blade is not wilted and is got final product; After the cuttage 30 days, refine seedling; After this, every at a distance from 15～20 days, impose once-combined fertilizer; When the nursery stock rugosity reaches 0.5cm, carry out graft seedling growth.
Specify as of the present invention, the present invention provides concrete technical scheme following: mainly by making, the nonwoven container for plant growth central authorities of the fertilizer in light ground mass preparation, the seedling medium, nonwoven container for plant growth irritate husky with the preparation of putting, inserting fringe making and cuttage, special microorganism preparation and technology such as use, manage after the cuttage form.Concrete steps are following:
(1) light ground mass preparation
The matrix body material is peat and perlite, carries out composite according to following part by weight:
Peat: 40-60 part
Perlite: 40-60 part;
After peat and perlite mixed in proportion, its weight was designated as S.
(2) fertilizer in the seedling medium
Be mainly urea, superphosphate, potassium chloride.The urea that adds 0.05-0.1%S weight respectively, the potassium chloride of the superphosphate of 0.3-0.5%S weight, 0.03-0.05%S weight.
(3) sterilization of matrix
The carbendazim that adds 0.08-0.12%S weight.
(4) making of nonwoven container for plant growth
Container for plant growth is cylindric, and the employing diameter is 5.8cm, highly is the nonwoven container of 10cm, carries out can production by the LKY-3 type forming machine of growing seedlings, and container formation, matrix can, container cutting are once accomplished.
(5) nonwoven container for plant growth central authorities irritate husky and put
In the matrix of container center, beat the hole of dark 3-4cm with the rifle bar of diameter 1-2cm, in the hole, fill river sand then.In special-purpose seedling growing box, special-purpose seedling growing box is placed on the removable seedbed vessel discharge, and special-purpose seedling growing box is built on stilts, utilizes air nature root relief.
(6) inserting fringe makes and cuttage
In 6～August, select semi-lignified branch living then, that growth is enriched to carry out cuttage, choose the middle and upper part of branch, rugosity is advisable at 0.3～0.5cm.Cutting is cut into the slotting fringe of growing 10～12cm, and radical leaves removes, and keeps a pair of blade in top, and blade is cut half, with soaking 1.5～3.5 hours in the ABT rooting powder solution of slotting fringe base portion with 150～300ppm that shears.
The slotting fringe of handling well is inserted in the river sand of container center, and the cuttage degree of depth is 3-4cm, and each container inserts a slotting fringe.
(7) preparation of special microorganism preparation with use
With the short living bacterium YT3 (deposit number is CGMCC No.5319) of the cherry rhizosphere of preserving; Activation on the LB inclined-plane, picking one articulating is gone in the beef-protein medium, in 37 ℃; Shaken cultivation is 2 days under the rotating speed 180rpm condition; Utilize colony counting method to measure every milliliter of bacteria containing amount, calculate bacterial concentration, be diluted to 1.0 * 10 then
8Cfu/mL.After cuttage 10-15 days, get the 10-20mL suspension and evenly water around cherry is inserted fringe.
(8) manage after the cuttage
The summer high temperature phase need be set up shady net and carried out sunshade, and shady net light transmittance is preferably 50% of full exposure; With intermittent automated spray system control moisture, in the cuttage 15～20 days, keep the blade face not do after the cuttage; After the cuttage 15 days, the new root of transplanting a cutting begins to take shape, control moisture, and the assurance blade is not wilted and is got final product; After the cuttage 30 days, refine seedling; After this, every at a distance from 15～20 days, impose once-combined fertilizer; When the nursery stock rugosity reaches 0.5cm, carry out graft seedling growth.
Disclosed by the inventionly utilize microorganism formulation and nonwoven container epicormic branch cuttage sweet cherry dwarfing rootstock technology compared with prior art, its beneficial effect is:
(1) sweet cherry dwarfing rootstock epicormic branch cuttage is beneficial to and takes root in the river sand of light matrix nonwoven container, and it is in the matrix of main material that the new root of formation penetrates with peat and vermiculite, is beneficial to root system and seedling growth; The volume of light matrix nonwoven container is bigger, for the growth of nursery stock provides the prescribing adequate nutrition area, still can grow more than 1 year therein behind the seedling grafting; Reduced cuttage seeding and transplanted link, not only reduced seedling cost, and significantly improved the quality of nursery stock.
(2) sweet cherry dwarfing rootstock epicormic branch cuttage nonwoven container seedling is in cultivating process; Root system propelling container wall also forms some sturdy callus after air is cut root, the root system self-sow forms the balance root system; Root system can closely interweave with light ground mass and be one, forms whippy root ball.Do not have seedling-slowing stage after the field planting, the primary growth amount significantly improves.
(3) use cherry special microorganism preparation after the cuttage, obviously promote the formation and the growth of cherry root system, compare with contrast, footpath and height of seedling significantly improve at the bottom of the cherry seedling, and the biomass of large cherry also significantly increases after the field planting.
Applying of this method will be brought bigger social benefit and economic benefit.
Embodiment
Through specific embodiment the present invention is further set forth below, should be noted that following explanation only is in order to explain the present invention, its content not to be limited.
Embodiment 1
One, light ground mass preparation
The prescription of matrix body material is: 50 kilograms in peat, 50 kilograms of perlites;
Fertilizer formula is: the weight in the matrix body material is S, and consumption is distinguished as follows: the urea of 0.05%S, the superphosphate of 0.3%S, the potassium chloride of 0.03%S;
Pasteurization material is a carbendazim, and the addition of carbendazim is 0.12% of S;
Host material, fertilizer and carbendazim is blended together by above-mentioned prescription, fully mix thoroughly;
Two, the making of nonwoven container for plant growth with put
Container for plant growth is cylindric, and the employing diameter is 5.8cm, highly is the nonwoven container of 10cm, carries out can production by the LKY-3 type forming machine of growing seedlings.
In the matrix of container center, beat the hole of dark 5cm with the rifle bar of diameter 2cm, in the hole, fill river sand then; In special-purpose seedling growing box, special-purpose seedling growing box is placed on the removable seedbed vessel discharge, and special-purpose seedling growing box is built on stilts.
Three, inserting fringe makes and cuttage
In June, the middle and upper part of selecting to give birth to semi-lignified branch then makes slotting fringe, and rugosity is at 0.3～0.5cm.Cutting is cut into the slotting fringe of long 10～12cm, and the upper end cuts flat, and the lower end is cut into shape of a hoof radical leaves to be removed, and keeps a pair of blade in top, and blade is cut half, with the slotting fringe base portion that shears with immersion in the ABT1 rooting powder solution of 200ppm 3 hours.
The slotting fringe of handling well is inserted in the river sand of container center, and the cuttage degree of depth is 3-4cm, and each container inserts a slotting fringe.
Four, the preparation of special microorganism preparation with use
With the short living bacterium YT3 (deposit number is CGMCC No.5319) of the cherry rhizosphere of preserving; Activation on the LB inclined-plane, picking one articulating is gone in the beef-protein medium, in 37 ℃; Shaken cultivation is 2 days under the rotating speed 180rpm condition; Utilize colony counting method to measure every milliliter of bacteria containing amount, calculate bacterial concentration, be diluted to 1.0 * 10 then
8Cfu/mL.
Five, manage after the cuttage
The summer high temperature phase need be set up shady net and carried out sunshade.In the cuttage 15～20 days, spraying and intermittent time because of one day intensity of illumination with temperature variation just set, generally be spray in morning of fine day 20 seconds/inferior, 7 minutes intermittences; Spray noon 10～20 seconds/inferior, intermittently 3 minutes; Spray afternoon 20 seconds/inferior, intermittently 5～6 minutes; Cloudy day spray 10 seconds/inferior, intermittently 10-15 minute; After a little longer cuttage of intermittent time at night with intermittent automated spray system control moisture.
After cuttage the 10th day, get 20mL special microorganism preparation suspension and evenly water around cherry is inserted fringe.
After the cuttage 15 days, spray 20 seconds/inferior, the intermittent time than the early stage longer slightly.After the cuttage 30 days, refine seedling, sooner or later every per 30 minutes of noon, water spray was 1 time, prolonged water spraying time at a distance from 1 hour water spray 1 time, sprayed permeablely at every turn, and increased ventilation, light transmission capacity.
The refining seedling is the new young sprout blade commentaries on classics strong green of sprouting after 15 days, after this, and when stromal surface is turned white; When water extruding matrix cannot see water; Water permeablely, every, spray mass percent and be 0.2% urea and mass percent and be 0.2% potassium dihydrogen phosphate mixed aqueous solution at a distance from 15～20 days; When the nursery stock rugosity reaches 0.5cm, carry out the sweet cherry graft seedling growth.
Rooting rate of this seedling-cultivating method and nursery effect test:
The test processing method:
Handle 1: this routine seedling-cultivating method adopts embodiment 1;
Handle 2: slotting machine seedling-cultivating method, seedling-cultivating method list of references " test of Gisela (Gisela) cherry rootstock epicormic branch cutting " (" Shandong forestry science and technology " 01 phase in 2010, old prime minister; Chen Kaihua; Li Shurong), seedling medium adopts the peat composed of rotten mosses: vermiculite: river sand=3:1:1;
Handle 3: the seedling medium employing " forest nonwoven container nursery biotype matrix (application number: 201110004733.9) ", not punching (not adding river sand) in container nursery matrix center, does not add the special microorganism preparation, and other are with embodiment 1;
Handle 4: adopt embodiment 1, but do not punch (not adding river sand) in container nursery matrix center;
Handle 5: adopt embodiment 1, but do not add the special microorganism preparation.
Tested on June 15th, 2011 and carry out, as supplying the examination material, choose the spray of giving birth to semi-lignified then with Gisela 6, every kind of processing method triplicate selects rugosity at 0.3～0.5cm.Cutting is cut into the slotting fringe of growing 10～12cm, with soaking 3 hours in the ABT1 rooting powder solution of 200ppm market purchase.The situation of taking root is checked in cuttage after 30 days, and the measurement average root is long and root is thick, and the result sees table 1.
Table 1
| Handle number | Root length/cm | Root is thick/mm | Rooting rate/% | Survival rate of seedling/% |
| 1 | 7.2a | 3.5a | 95.3a | 89.8a |
| 2 | 6.6b | 2.8bc | 86.6b | 80.3b |
| 3 | 6.4b | 2.6c | 67.6c | 60.1c |
| 4 | 7.0a | 3.0b | 66.4c | 57.9c |
| 5 | 6.4b | 2.8bc | 94.3a | 83.2b |
The different lowercases of same column are represented different disposal differences significantly (P < 0.05)
With sand table commonly used grow seedlings (handling 2) compare, the falsesour cherry root of this routine seedling-cultivating method is long, root is thick, rooting rate and survival rate of seedling significant difference, has improved 9.09%, 25.0%, 10.05% and 11.83% respectively.
Compare with handling 3, the falsesour cherry root of this routine seedling-cultivating method is long, root is thick, rooting rate and survival rate of seedling have improved 12.5%, 34.62%, 40.98% and 49.42% respectively.
Embodiment does not punch at 1 center and does not add river sand (handle 4), and root is thick, rooting rate and survival rate of seedling have reduced by 14.29%, 30.33% and 35.52% respectively than embodiment 1
Embodiment 1 does not add special microorganism preparation (handling 5), and root is long, root is thick and survival rate of seedling has reduced by 11.11%, 20.00% and 9.11% respectively than embodiment 1.
Can know by the above result of growing seedlings, the punching of nonwoven container center, and increased substantially rooting rate after adding river sand.In seedling medium, add the short endophytic bacteria of YT3 cherry rhizosphere, though less to the rooting rate influence, mentioned nursery effect significantly.Sand table seedling-cultivating method with commonly used is compared, and the rooting rate and the nursery effect of this routine seedling-cultivating method all are improved to some extent.
Though above-mentioned combination embodiment specific embodiments of the invention is described; But be not restriction to protection domain of the present invention; On the basis of technical scheme of the present invention, those skilled in the art need not pay various modifications that creative work can make or distortion still in protection scope of the present invention.
Claims (10)
1. base composition that is used for sweet cherry dwarfing rootstock epicormic branch cutting is characterized in that it comprises:
(1) matrix body material: the matrix body material is made up of the raw material of following weight portion, peat: 40-60 part, perlite: 40-60 part;
(2) fertilizer: fertilizer is S in the weight of matrix body material, and consumption is distinguished as follows: the urea of 0.05-0.1%S weight, the potassium chloride of the superphosphate of 0.3-0.5%S weight, 0.03-0.05%S weight;
(3) microorganism formulation: with the short living bacterium YT3 of cherry rhizosphere, deposit number is CGMCC No.5319, after the activated cultivation, is diluted to 1.0 * 10
8Cfu/mL.
2. sweet cherry dwarfing rootstock epicormic branch cuttage nonwoven container seedling culture method is characterized in that concrete steps are following:
(1) matrix for preparing is filled in the nonwoven container for plant growth, will inserts fringe afterwards and insert in the matrix;
(2) be the short living bacterium YT3 of cherry rhizosphere of CGMCC No.5319 with deposit number, obtain suspension after the activation culture, get suspension and evenly water around slotting fringe;
(3) the laggard administration-management reason of cuttage is grown seedlings.
3. method according to claim 2 is characterized in that said matrix comprises matrix body material and fertilizer; Wherein the matrix body material is made up of the raw material of following weight portion; Peat: 40-60 part, perlite: 40-60 part, fertilizer is S in the weight of matrix body material; Consumption is distinguished as follows: the urea of 0.05-0.1%S weight, the potassium chloride of the superphosphate of 0.3-0.5%S weight, 0.03-0.05%S weight.
4. method according to claim 3 is characterized in that, it also comprises the process to the matrix sterilization, promptly in matrix body material and fertilizer, adds the carbendazim of 0.08-0.12%S weight, and is blended together, fully mixes thoroughly.
5. method according to claim 2 is characterized in that, be filled to matrix in the nonwoven container for plant growth after, in the matrix of container center, punch, and in the hole, fill river sand, will insert then in the river sand that fringe inserts container center.
6. want 5 described methods according to right, it is characterized in that, said nonwoven container for plant growth is cylindric, and diameter is 5.8cm, highly is 10cm that the rifle bar of employing diameter 1-2cm is beaten the hole of dark 3-4cm in the matrix of container center, in the hole, fill river sand then; In the time of in the river sand of slotting fringe insertion container center, the cuttage degree of depth is 3-4cm.
7. method according to claim 2 is characterized in that, said slotting fringe processing method is following: in 6～August, select semi-lignified branch living then, that growth is enriched to carry out cuttage, choose the middle and upper part of branch, rugosity is advisable at 0.3～0.5cm; Cutting is cut into the slotting fringe of growing 10～12cm, and radical leaves removes, and keeps a pair of blade in top, and blade is cut half, with soaking 1.5～3.5 hours in the ABT rooting powder solution of slotting fringe base portion with 150～300ppm that shears.
8. method according to claim 2 is characterized in that, is the short living bacterium YT3 of cherry rhizosphere of CGMCC No.5319 with the deposit number of preserving; Activation on the LB inclined-plane, picking one articulating is gone in the beef-protein medium, in 37 ℃; Shaken cultivation is 2 days under the rotating speed 180rpm condition; Utilize colony counting method to measure every milliliter of bacteria containing amount, calculate bacterial concentration, be diluted to 1.0 * 10 then
8Cfu/mL; After cuttage 10-15 days, get the 10-20mL suspension and evenly water around cherry is inserted fringe.
9. method according to claim 2 is characterized in that, the method that the laggard administration-management reason of said cuttage is grown seedlings is: the summer high temperature phase need be set up shady net and carried out sunshade, and shady net light transmittance is preferably 50% of full exposure; With intermittent automated spray system control moisture, in the cuttage 15～20 days, keep the blade face not do after the cuttage; After the cuttage 15 days, the new root of transplanting a cutting begins to take shape, control moisture, and the assurance blade is not wilted and is got final product; After the cuttage 30 days, refine seedling; After this, every at a distance from 15～20 days, impose once-combined fertilizer; When the nursery stock rugosity reaches 0.5cm, carry out graft seedling growth.
10. method according to claim 2 is characterized in that, said step is specially:
(1) light ground mass preparation
The matrix body material is peat and perlite, carries out composite according to following part by weight:
Peat: 40-60 part
Perlite: 40-60 part
After peat and perlite mixed in proportion, its weight was designated as S;
(2) fertilizer in the seedling medium
Be mainly urea, superphosphate, potassium chloride, add the urea of 0.05-0.1%S weight respectively, the potassium chloride of the superphosphate of 0.3-0.5%S weight, 0.03-0.05%S weight;
(3) sterilization of matrix
The carbendazim that adds 0.08-0.12%S weight; Host material, fertilizer and carbendazim is blended together by above-mentioned prescription, fully mix thoroughly;
(4) making of nonwoven container for plant growth
Container for plant growth is cylindric, and the employing diameter is 5.8cm, highly is the nonwoven container of 10cm, carries out can production by the LKY-3 type forming machine of growing seedlings, and container formation, matrix can, container cutting are once accomplished;
(5) nonwoven container for plant growth central authorities irritate husky and put
In the matrix of container center, beat the hole of dark 3-4cm with the rifle bar of diameter 1-2cm, in the hole, fill river sand then; In special-purpose seedling growing box, special-purpose seedling growing box is placed on the removable seedbed vessel discharge, and special-purpose seedling growing box is built on stilts, utilizes air nature root relief;
(6) inserting fringe makes and cuttage
In 6～August, select semi-lignified branch living then, that growth is enriched to carry out cuttage, choose the middle and upper part of branch, rugosity is advisable at 0.3～0.5cm; Cutting is cut into the slotting fringe of growing 10～12cm, and radical leaves removes, and keeps a pair of blade in top, and blade is cut half, with soaking 1.5～3.5 hours in the ABT rooting powder solution of slotting fringe base portion with 150～300ppm that shears;
The slotting fringe of handling well is inserted in the river sand of container center, and the cuttage degree of depth is 3-4cm, and each container inserts a slotting fringe;
(7) preparation of special microorganism preparation with use
With deposit number is the short living bacterium YT3 of cherry rhizosphere of CGMCC No.5319; Activation on the LB inclined-plane, picking one articulating is gone in the beef-protein medium, in 37 ℃; Shaken cultivation is 2 days under the rotating speed 180rpm condition; Utilize colony counting method to measure every milliliter of bacteria containing amount, calculate bacterial concentration, be diluted to 1.0 * 10 then
8Cfu/mL; After cuttage 10-15 days, get the 10-20mL suspension and evenly water around cherry is inserted fringe;
(8) manage after the cuttage
The summer high temperature phase need be set up shady net and carried out sunshade, and shady net light transmittance is preferably 50% of full exposure; With intermittent automated spray system control moisture, in the cuttage 15～20 days, keep the blade face not do after the cuttage; After the cuttage 15 days, the new root of transplanting a cutting begins to take shape, control moisture, and the assurance blade is not wilted and is got final product; After the cuttage 30 days, refine seedling; After this, every at a distance from 15～20 days, impose once-combined fertilizer; When the nursery stock rugosity reaches 0.5cm, carry out graft seedling growth.
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| CN (1) | CN102742495B (en) |
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| CN103385148A (en) * | 2013-07-10 | 2013-11-13 | 张喜平 | Cherry stunting and condensed planting technology |
| CN104798670A (en) * | 2015-04-30 | 2015-07-29 | 山东省林业科学研究院 | Non-woven fabric container cutting seedling raising method of Fraxinus velutina twigs |
| CN105325232A (en) * | 2015-10-10 | 2016-02-17 | 中国农业科学院郑州果树研究所 | Cherry stock cuttage fast breeding method |
| CN105557435A (en) * | 2016-01-19 | 2016-05-11 | 江苏阿里巴巴农业科技有限公司 | Cherry rootstock cuttage method |
| CN105993791A (en) * | 2016-06-03 | 2016-10-12 | 合肥原印农业科技有限公司 | High-yield greenhouse planting method for Chinese cherries |
| CN106472224A (en) * | 2016-10-13 | 2017-03-08 | 合肥润雨农业科技有限公司 | A kind of cuttage and seedling culture method of european large cherry |
| CN107027574A (en) * | 2017-04-25 | 2017-08-11 | 孙廷� | A kind of smooth leaf paper mulberry green branch cuttage breeding method |
| CN107409911A (en) * | 2017-08-08 | 2017-12-01 | 合肥满地金农业科技有限公司 | A kind of implantation methods for improving sapindoside content |
| CN107771601A (en) * | 2017-09-26 | 2018-03-09 | 安徽徽大农业有限公司 | A kind of method for culturing seedlings of gean |
| CN111406761A (en) * | 2020-05-14 | 2020-07-14 | 河北吉瑞馨农业科技发展有限公司 | Preparation method of cherry tree transplanting rooting agent |
| CN114557271A (en) * | 2022-01-19 | 2022-05-31 | 湖北格林凯尔农业科技有限公司 | Root-limiting soilless culture technology for red-heart kiwi fruits |
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| CN103229647B (en) * | 2013-04-03 | 2015-01-07 | 汉中亨泰生物科技有限公司 | Method for propagating Taxus madia nursery stocks |
| CN103229647A (en) * | 2013-04-03 | 2013-08-07 | 汉中亨泰生物科技有限公司 | Method for propagating Taxus madia nursery stocks |
| CN103385148A (en) * | 2013-07-10 | 2013-11-13 | 张喜平 | Cherry stunting and condensed planting technology |
| CN104798670B (en) * | 2015-04-30 | 2017-03-08 | 山东省林业科学研究院 | A kind of Fraxinus velutina twig non-woven container cuttage and seedling culture method |
| CN104798670A (en) * | 2015-04-30 | 2015-07-29 | 山东省林业科学研究院 | Non-woven fabric container cutting seedling raising method of Fraxinus velutina twigs |
| CN105325232B (en) * | 2015-10-10 | 2018-06-29 | 中国农业科学院郑州果树研究所 | A kind of cherry root stock cutting fast breeding method |
| CN105325232A (en) * | 2015-10-10 | 2016-02-17 | 中国农业科学院郑州果树研究所 | Cherry stock cuttage fast breeding method |
| CN105557435A (en) * | 2016-01-19 | 2016-05-11 | 江苏阿里巴巴农业科技有限公司 | Cherry rootstock cuttage method |
| CN105993791A (en) * | 2016-06-03 | 2016-10-12 | 合肥原印农业科技有限公司 | High-yield greenhouse planting method for Chinese cherries |
| CN106472224A (en) * | 2016-10-13 | 2017-03-08 | 合肥润雨农业科技有限公司 | A kind of cuttage and seedling culture method of european large cherry |
| CN107027574A (en) * | 2017-04-25 | 2017-08-11 | 孙廷� | A kind of smooth leaf paper mulberry green branch cuttage breeding method |
| CN107027574B (en) * | 2017-04-25 | 2020-06-09 | 孙廷� | Broussonetia papyrifera green branch cutting propagation method |
| CN107409911A (en) * | 2017-08-08 | 2017-12-01 | 合肥满地金农业科技有限公司 | A kind of implantation methods for improving sapindoside content |
| CN107771601A (en) * | 2017-09-26 | 2018-03-09 | 安徽徽大农业有限公司 | A kind of method for culturing seedlings of gean |
| CN111406761A (en) * | 2020-05-14 | 2020-07-14 | 河北吉瑞馨农业科技发展有限公司 | Preparation method of cherry tree transplanting rooting agent |
| CN114557271A (en) * | 2022-01-19 | 2022-05-31 | 湖北格林凯尔农业科技有限公司 | Root-limiting soilless culture technology for red-heart kiwi fruits |
| CN114557271B (en) * | 2022-01-19 | 2023-09-05 | 湖北格林凯尔农业科技有限公司 | Root limiting soilless cultivation technology for red kiwi fruits |
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