The cuttage breeding method of south blue berry
Technical field
The invention belongs to blue berry reproduction technique field, particularly relate to the cuttage breeding method of a kind of blue berry.
Background technology
Blue berry belongs to Ericaceae Vacciniodeae genus vaccinium plant, has kind more than 400, and it is distributed widely in the Northern Hemisphere.China's blue berry
Producing cultivation and be still in the starting stage, from Jilin, Shandong, Liaoning etc. saves and gradually develops into the south of the lower reaches of the Yangtze River.Blue berry is a kind of nutrition
Being worth high novel fruits, economic worth is high, has good development prospect, but in industrialization produces, blue berry breeding one
It is directly a great problems that face of those skilled in the art.Blue berry typically based on cottage propagation, the more general little slurry of blue berry cottage propagation
Fruit tree survival rate is low, and technology requires height, in all parts of the country extensive to the research of blue berry cuttage technique.Shadow due to climatic environmental factor
Ringing, the same kind cutting plantation difference in different regions is bigger.
The commonly used following operation of conventional cottage propagation:
1, the selection of cutting medium: main employing plurality of raw materials is prepared, and material composition contains river sand, sawdust, the peat composed of rotten mosses, curdy fur
Moss, perlite etc., and disinfect with 500~600 times of carbendazim;
2, the selection of root-growing agent: conventional root-growing agent typically with 2000~3000ml/l naphthalene acetic acid, this root-growing agent process blue berry
Branch DeGrain, rooting rate ratio is relatively low.
3, the selection in cuttage period: be typically selected in 3~June cutting experiment preferable.
CN102138529A Chinese patent literature is referred to the blueberry cultivation using naphthalene acetic acid and heteroauxing to compound composition use
Root-growing agent, it mentions that the root-growing agent formula of use is naphthalene acetic acid and heteroauxing, and is to be directly dissolved in water, but from naphthalene acetic acid,
From the point of view of the physicochemical property of heteroauxing, they are all to be insoluble in water, be soluble in the solid matter of organic solvent, and experiment also indicates that,
Naphthalene acetic acid and heteroauxing dissolve insufficient in water, have precipitation granule, it is difficult to reach the effect of dispersed and effective utilization.
CN102613078 Chinese patent literature is referred to the method using indolebutyric acid to make blue berry Rapid Rooting as root-growing agent, its
Using and dissolve indolebutyric acid with NaOH, use water constant volume, but experiment shows, the rooting efficiency of alone indolebutyric acid more has
Limit, and still have precipitation after NaOH dissolving, the constant volume that adds water, do not reach homodisperse effect.It addition, this root-growing agent master
If being applied to tissue cultured seedling, it is not applied to cuttage branch example.The life mentioned in CN102668853A Chinese patent literature
Root agent preparation is with water as lysate, and indolebutyric acid is dissolved insufficient by water, and placement a few minutes will precipitate, and does not reaches
Dispersed effect, further mentions in the document and have employed the peat composed of rotten mosses in substrate of taking root, but in peat composed of rotten mosses substrate, pathogenic bacteria is the most, uses
Time as thorough in sterilization, serious disease pest will be brought to endanger cutting seedling, affects blue berry Seedling quality.
Make solvent with water it addition, existing root-growing agent compound method is usually or is first dissolved in ethanol and uses water constant volume, this kind of method again
Do not consider the sedimentation problem after fully the dissolving and dissolve of solute, reduce the effectiveness of root-growing agent;Use additionally, dissolve with ethanol
Water constant volume, ethanol is difficult to distribute, and to root system of plant and root upper part all it may happen that injury in cuttage, reduces further
The useful effect of root-growing agent.
Visible, drawbacks described above the most all can have influence on the application effect of root-growing agent and the successful reproduction of blue berry.
Summary of the invention
The technical problem to be solved is, overcomes the deficiency and defect mentioned in background above technology, it is provided that a kind of step
Simply, rooting rate and planting percent is higher, low cost, the cuttage breeding method of southern blue berry easily promoted.
For solve above-mentioned technical problem, the present invention propose technical scheme be a kind of south blue berry cuttage breeding method, including with
Lower step:
A prepared by () cutting: select annual in more than 3 years age blue berry tree, semi-lignified, healthy and strong branch without pest and disease damage,
Cutting to put in the bucket filling water makes it not be dehydrated, and branch segmentation is cut into the cutting of certain length in the cool, cutting is put
Enter in water container standby;
B () substrate prepares: inserted by cutting medium in cuttage railway carriage or compartment, trickle drenched leveling compacting, naturally dries in the shade, with spray before cuttage
Mist is sterilized;
C () cuttage: in first arrival in August by the end of September, chooses above-mentioned standby cutting, cutting base portion is stained with powder of taking root, insert
In substrate, each cutting keeps a determining deviation;
(d) water spray management: the cutting after above-mentioned cuttage is carried out water spray management, use thin spray pattern water spray (never water,
Spraying can use agricultural atomizer or automatic spraying system), adjust day spray timings according to temperature difference, and keep canopy humidity to exist
80%~90%, soil humidity, 65%~75%, continues at least 20 days, when cutting base portion starts to bear callus, by
Step reduces day spray timings, keeps substrate to see and dry sees wet, and after cuttage at least 50 days, the root that makes new advances grow in cutting substantially, and newly branch and leaf are long
Gesture is good.
Above-mentioned cuttage breeding method, it is preferred that in described step (a), the cutting length being cut into controls at 10cm~12cm,
And in each cutting, keep more than 2 leaf buds.
Above-mentioned cuttage breeding method, it is preferred that in described step (b), the substrate of described cuttage selects pure perlite, spraying
500 times of liquid carbendazim are selected in sterilization.The cutting medium that present invention preferably uses is pure perlite (i.e. without other compositions), its tool
Have the good permeability, sterilization convenient, thoroughly, it is to avoid adding other materials is easily carried the problem of pathogenic bacteria, can ensure that cutting seedling without
Courses of infection, seedling is healthy and strong.
Above-mentioned cuttage breeding method, it is preferred that in described step (c), inserts the degree of depth at least 3cm of substrate, each cutting
Spacing is maintained at 6cm~10cm.
Above-mentioned cuttage breeding method, it is preferred that in described step (d), adjusts day spray timings according to temperature difference and refers to:
At least sprayed 1 time every 2 hours during megathermal climate without rain;Temperature, when 25 DEG C~30 DEG C, was at least sprayed 1 time every 4 hours;
Spraying duration controls at 10s~30s every time.
Above-mentioned cuttage breeding method, it is preferred that described in powder of taking root be compound powder of taking root, described compound powder of taking root
Mixed by powder A and powder B is compound;
Described powder A is mainly formulated by following mass fraction component:
Naphthalene acetic acid: 1~2 part;
Pulvis Talci: 1000 parts;
Described powder B is mainly formulated by following mass fraction component:
Indolebutyric acid: 0.5~1 part;
Pulvis Talci: 1000 parts.
Above-mentioned cuttage breeding method, it is preferred that mass ratio during described powder A and powder B mixing is 2~1: 1.
Above-mentioned cuttage breeding method, it is preferred that described Pulvis Talci is the industrial talc powder of 1200 mesh.The Pulvis Talci of the present invention belongs to
In auxiliary agent, Pulvis Talci is used not only to draw materials as auxiliary agent easily, and material non-toxic, tasteless, cuttage branch is not had any
Physics and chemical affect, use is convenient to.It addition, the present invention selects the industrial talc powder of 1200 mesh as naphthalene acetic acid and Yin
The auxiliary agent of diindyl butanoic acid, the Pulvis Talci being on the one hand because this granularity is the finest and the smoothest, grinds and mixes the uniformity very well, does not interferes with root-inducing powder
The effect of agent, on the other hand the Pulvis Talci of this granularity is the most more economically.
The most compound above-mentioned powder of taking root is based primarily upon following principle: first, it is settled that selected two kinds of main constituent materials, a kind of
It is naphthalene acetic acid (1-Naphthaleneacetic acid), is called for short NAA, be a kind of organic compound (C10H7CH2CO2H), belong to raw
Long element class plant modifying agent, in addition to having the basic function of general auxin, it is also possible to promote plant adventitious root and the formation of root.
But, our research indicate that, alone naphthalene acetic acid though crop rooting promoting is good, but under higher concentration, have the suppression aerial stem of plant, branch
The side effect of growth, Seedling growth is undesirable.Another kind of main constituent is indolebutyric acid, and molecular formula is C12H13NO2, platymiscium is raw
Long regulator, it is mainly used in rooting of cuttings, can induce the formation of root substance, promotes cell differentiation and division, the newest root
Growth and the differentiation of fibrovascular system, promote cutting adventitious root formation.Indolebutyric acid promotes that the effect of plant root of hair not only shows
Work is better than heteroauxing, and stable in properties, is difficult to conduction in vivo, only rests on process position, uses safer.By inciting somebody to action
Both the above main constituent compounds, and can not only effectively utilize the technical advantage of each composition, is formed and has complementary advantages, and can keep away
Exempt from the higher side effect that can suppress aerial growth of single component concentration and concentration is relatively low to inspiring the unconspicuous difficulty of root growth
Topic;Again by using Pulvis Talci to make auxiliary agent, make the solid-state of present invention powder of taking root can dilute uniformly, be avoided that again organic solvent
Injury to root.
Above-mentioned cuttage breeding method, it is preferred that the compound method of described compound powder of taking root comprises the following steps:
1) take the naphthalene acetic acid of described mass parts, use a small amount of anhydrous alcohol solution, then add a certain amount of Pulvis Talci and make itself and naphthalene second
Acid ethanol solution fully mix thoroughly, naturally dry in the shade so that it is in ethanol volatilize completely, then deposit in the containers for future use of lucifuge,
Obtain powder A;
2) take the indolebutyric acid of described mass parts, use a small amount of anhydrous alcohol solution, the Pulvis Talci then adding residual mass part to make it
Fully mix thoroughly with the ethanol solution of indolebutyric acid, naturally dry in the shade so that it is in ethanol volatilize completely, then deposit in the appearance of lucifuge
In device standby, obtain powder B;
3) take powder A and the powder B mixing of respective quality ratio, obtain described compound powder of taking root.
In the compound method of the invention described above, owing to formula material is insoluble in water, it is soluble in organic solvent, so using a small amount of nothing
Water-ethanol can dissolve fully, naturally dries in the shade, make ethanol volatilize completely after mixing thoroughly with Pulvis Talci, be because dehydrated alcohol to branch and
Root has injury effect, and therefore, it is thorough that this collocation method all considers from the utilization of action principle and active substance, has reached good effect
Really.
Above-mentioned cuttage breeding method, it is furthermore preferred that described step 1) in talcous consumption and described step 2) in Pulvis Talci
Consumption essentially identical.
Above-mentioned cuttage breeding method, it is preferred that when blue berry is carried out cuttage, now takes described powder A and the powder of equal quality
Agent B puts in container and fully mixes thoroughly, controls every thousand strain cuttings and uses powder A and each 30g of powder B;By cutting base portion during cuttage
Compound powder of taking root after mixing thoroughly described in being stained with, is inserted into substrate.
Compared with prior art, it is an advantage of the current invention that:
1) cuttage breeding method of the present invention is simple to operate, all changes cutting time, cutting medium, water spray control measures etc.
Entering and optimize, this makes the blue berry rooting rate after cottage propagation and planting percent higher;
2) present invention is also optimized improvement to the formula of powder of taking root, and significantly improves the effect after root-growing agent processes blue berry branch
Really, rooting rate and the planting percent of blue berry cuttage are further increased;
3) process for preparation of the compound powder of taking root of the present invention is simple, and easy to operate, cost is relatively low, is suitable to be widely popularized and apply;
4) variety culture of blue berry and cottage propagation under the conditions of the compound powder of taking root of the present invention is particularly well-suited to southern climates.
5) the compound powder of taking root of the present invention can be applied not only to blue berry, and also can be widely used to that other cuttages are difficult to take root plants
Thing (such as Chinese rose etc.), promotees rooting efficiency substantially (seeing Fig. 2).
Accompanying drawing explanation
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or prior art
In description, the required accompanying drawing used is briefly described, it should be apparent that, the accompanying drawing in describing below is some realities of the present invention
Execute example, for those of ordinary skill in the art, on the premise of not paying creative work, it is also possible to according to these accompanying drawings
Obtain other accompanying drawing.
Fig. 1 is the outward appearance photo of the compound powder of taking root of preparation in the specific embodiment of the invention.
Fig. 2 is the rooting efficiency contrast photo in the specific embodiment of the invention after the branch of compound powder process cuttage of taking root,
Wherein upper row's branch be the present invention take root powder process after rooting efficiency, lower row's branch is the effect of taking root after conventional root-growing agent processes
Really.
Fig. 3 is the growth result photo after the compound powder process cuttage blue berry of taking root of preparation in the specific embodiment of the invention.
Fig. 4 is the growth result photo after the root-growing agent being formulated conventionally processes cuttage blue berry.
Fig. 5 is to use the pure perlite of the present invention as the reproductive effect photo of cutting medium.
Fig. 6 is to use the conventional peat composed of rotten mosses as the reproductive effect photo of cutting medium.
Fig. 7 is to use conventional earth as the reproductive effect photo of cutting medium.
Detailed description of the invention
For the ease of understanding the present invention, below in conjunction with Figure of description and preferred embodiment, the present invention is made more comprehensively, careful
Ground describes, but protection scope of the present invention is not limited to embodiment in detail below.
Unless otherwise defined, the implication that all technical term used hereinafter is generally understood that with those skilled in the art is identical.
Technical term used herein is intended merely to describe the purpose of specific embodiment, is not intended to limit the protection model of the present invention
Enclose.
Unless otherwise specified, the various raw materials used in the present invention, reagent, instrument and equipment etc. all can be purchased by market
Can buy or can be prepared by existing method.
Embodiment:
The cuttage breeding method of the southern blue berry of a kind of present invention, comprises the following steps:
A prepared by () cutting: select annual in more than 3 years age blue berry tree, semi-lignified, healthy and strong branch without pest and disease damage,
Cutting to put in the bucket filling water makes it not be dehydrated, and branch segmentation is cut into the cutting of 10cm~12cm in the cool, and often
Keep more than 2 leaf buds in individual cutting, cutting is put in water container standby;
B () substrate prepares: inserted by cutting medium in cuttage railway carriage or compartment, and the substrate of cuttage selects pure perlite, trickle drenched leveling pressure
Real, naturally dry in the shade one day, with 500 times of liquid carbendazim spraying disinfections before cuttage;
C () cuttage: in first arrival in August by the end of September, chooses above-mentioned standby cutting, cutting base portion is stained with powder of taking root, insert
3cm in substrate, each cutting keeps the spacing of about 8cm;
(d) water spray management: the cutting after above-mentioned cuttage is carried out water spray management, use thin spray pattern water spray (never water,
Spraying can use agricultural atomizer or automatic spraying system), according to temperature difference adjust day spray timings, without rain megathermal climate up to
Few every spraying in 2 hours 1 time;Temperature, when 25 DEG C~30 DEG C, was at least sprayed 1 time every 4 hours;Spraying duration control every time
System is at 10s~30s, and keeps canopy humidity 80%~90%, and soil humidity, 65%~75%, continues at least 20 days, treats
Cutting occurs that when Lao Ye part leaf bud withered and yellow, new starts to sprout, cutting base portion starts to bear callus, gradually reduces day spraying
Number of times, keep substrate see dry see wet, the long root that makes new advances of cutting basic (at least 80%~90%) after cuttage at least 50 days, and newly
Branch and leaf grow fine.
The powder of taking root used in above-mentioned the present embodiment is compound powder of taking root, as it is shown in figure 1, it is to be 1: 1 by mass ratio
Powder A and powder B is compound mixes;
Powder A is mainly formulated by following mass fraction component:
Naphthalene acetic acid: 2 parts (1~2 part);
Pulvis Talci: 1000 parts;
Powder B is mainly formulated by following mass fraction component:
Indolebutyric acid: 1 part;
Pulvis Talci: 1000 parts, Pulvis Talci is the industrial talc powder of 1200 mesh.
The compound method of the above-mentioned compound powder of taking root of the present embodiment, comprises the following steps:
1) taking 1g naphthalene acetic acid, use a small amount of anhydrous alcohol solution, the Pulvis Talci then adding 500g makes it molten with the ethanol of naphthalene acetic acid
Liquid is fully mixed thoroughly, naturally dries in the shade so that it is in ethanol volatilize completely, then deposit in the containers for future use of lucifuge, obtain powder A;
2) taking 0.5g indolebutyric acid, use a small amount of anhydrous alcohol solution, the Pulvis Talci then adding 500g makes itself and indolebutyric acid
Ethanol solution is fully mixed thoroughly, naturally dries in the shade so that it is in ethanol volatilize completely, then deposit in the containers for future use of lucifuge,
Powder B;
3) take powder A and powder B mixing that mass ratio is 1: 1, obtain compound powder of taking root.
When blue berry is carried out cuttage, the powder A and powder B that now take homogenous quantities put in container and fully mix thoroughly, control every thousand strains
Cutting uses powder A and each 30g of powder B;Compound powder of taking root after mixing thoroughly described in cutting base portion being stained with during cuttage, so
Rear insertion substrate.
Compound powder A and the conventional naphthalene acetic acid root-growing agent B of taking root for preparing with above-mentioned the present embodiment (concentration be 2000~
3000ml/l) do cottage propagation Experimental Comparison, process the blue berry branch of cuttage with the compound powder A of taking root of the present embodiment after,
The high clump blue berry kind rooting rate in south reaches more than 90%, and planting percent reaches more than 80%;Vaccinium ashei cutting plantation more than 80%,
Planting percent reaches 70%;Compared with conventional naphthalene acetic acid root-growing agent B, the blue berry cutting plantation of the present invention improves 40%, becomes
Seedling rate improves the effect (contrast test effect see table 1) of 35%.Fig. 3 gives the compound powder of taking root of preparation and processes cuttage
Growth result photo after blue berry, Fig. 4 is the growth result photo after the root-growing agent being formulated conventionally processes cuttage blue berry, comparison diagram
3 and Fig. 4 is visible, and the blue berry growth after the present invention processes is in great numbers, and rooting rate and planting percent significantly improve, and conventional root-growing agent processes
After blue berry growth sparse, planting percent is the highest.
Table 1: the compound powder A contrast test effect with conventional root-growing agent B of taking root of the present embodiment preparation
Table note: in upper table 1, each kind and each test strain number that processes are 500 strains.
Through us contrast test repeatedly and field experiment, the cutting time of south blue berry exists with blueberry cultivation elsewhere
Very big difference, in the present invention, time with late summer to the autumn of southern area cuttage blue berry is optimal, as the test in table 2 below is tied
Shown in fruit, the first arrival in August particularly preferably mentioned in above-described embodiment by the end of September because temperature now, humidity, illumination etc.
Environmental condition is most widely suited, and rooting rate and planting percent are high, and this not only effectively reduces material and cost of labor, and convenient management,
Be suitable to promote.
Table 2: the comparative test result of blue berry cutting time of the present invention
Table note: in upper table 2, each kind and each test strain number that processes are 500 strains.
Through us contrast test repeatedly and field experiment, it has been found that different cutting mediums also can significantly affect cuttage
Reproductive effect, the compositions being used mostly various complexity in prior art is also considered as cuttage base as cutting medium, traditional thinking
Matter composition is the most, and nutritive validity is the best, and actually this is not so, and as shown in the result of the test in table 3 below, different cutting mediums has
Respective pluses and minuses, a lot of cutting mediums also can introduce various antagonism composition and pathogenic bacterium, therefore particularly preferably make in the present invention
With pure perlite as cutting medium to strengthen the cottage propagation effect of blue berry further.As shown in Fig. 5~Fig. 7, in the present invention
Pure perlite is used to show optimal reproductive effect as the blue berry of cutting medium.
Table 3: the comparative test result of blue berry cuttage different substrates of the present invention
Table note: in upper table 3, each kind and each test strain number that processes are 500 strains.