CN109618851A - A kind of method of pueraria lobata nursery - Google Patents
A kind of method of pueraria lobata nursery Download PDFInfo
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- CN109618851A CN109618851A CN201811561316.2A CN201811561316A CN109618851A CN 109618851 A CN109618851 A CN 109618851A CN 201811561316 A CN201811561316 A CN 201811561316A CN 109618851 A CN109618851 A CN 109618851A
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- seedling
- nursery
- pueraria lobata
- chelating
- water
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- 235000010575 Pueraria lobata Nutrition 0.000 title claims abstract description 50
- 238000000034 method Methods 0.000 title claims abstract description 45
- 241000219781 Pueraria montana var. lobata Species 0.000 title abstract 6
- 239000007788 liquid Substances 0.000 claims abstract description 39
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 33
- 239000006228 supernatant Substances 0.000 claims abstract description 20
- 238000002360 preparation method Methods 0.000 claims abstract description 18
- 230000008569 process Effects 0.000 claims abstract description 9
- 244000046146 Pueraria lobata Species 0.000 claims description 44
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 44
- 239000000243 solution Substances 0.000 claims description 34
- 150000001413 amino acids Chemical class 0.000 claims description 28
- 239000003337 fertilizer Substances 0.000 claims description 23
- 229910052708 sodium Inorganic materials 0.000 claims description 21
- 239000011734 sodium Substances 0.000 claims description 21
- 229940077388 benzenesulfonate Drugs 0.000 claims description 19
- -1 sodium alkyl benzene Chemical class 0.000 claims description 19
- 238000000855 fermentation Methods 0.000 claims description 18
- 230000004151 fermentation Effects 0.000 claims description 18
- 239000012452 mother liquor Substances 0.000 claims description 18
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 14
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 14
- 239000006013 carbendazim Substances 0.000 claims description 14
- 230000012010 growth Effects 0.000 claims description 14
- 241000345998 Calamus manan Species 0.000 claims description 13
- 239000012895 dilution Substances 0.000 claims description 13
- 238000010790 dilution Methods 0.000 claims description 13
- 235000012950 rattan cane Nutrition 0.000 claims description 13
- 239000007921 spray Substances 0.000 claims description 12
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 claims description 11
- 229960001471 sodium selenite Drugs 0.000 claims description 11
- 235000015921 sodium selenite Nutrition 0.000 claims description 11
- 239000011781 sodium selenite Substances 0.000 claims description 11
- 238000005507 spraying Methods 0.000 claims description 11
- 235000007119 Ananas comosus Nutrition 0.000 claims description 9
- 230000000844 anti-bacterial effect Effects 0.000 claims description 9
- 239000003899 bactericide agent Substances 0.000 claims description 9
- 239000006041 probiotic Substances 0.000 claims description 9
- 235000018291 probiotics Nutrition 0.000 claims description 9
- 150000005846 sugar alcohols Chemical class 0.000 claims description 9
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims description 8
- 235000017491 Bambusa tulda Nutrition 0.000 claims description 8
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims description 8
- 239000011425 bamboo Substances 0.000 claims description 8
- 239000000052 vinegar Substances 0.000 claims description 8
- 235000021419 vinegar Nutrition 0.000 claims description 8
- 238000009333 weeding Methods 0.000 claims description 8
- 241001116389 Aloe Species 0.000 claims description 7
- 244000050510 Cunninghamia lanceolata Species 0.000 claims description 7
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 7
- 235000011399 aloe vera Nutrition 0.000 claims description 7
- 239000004202 carbamide Substances 0.000 claims description 7
- 150000002596 lactones Chemical class 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 7
- 239000000843 powder Substances 0.000 claims description 7
- 235000000346 sugar Nutrition 0.000 claims description 7
- 230000009514 concussion Effects 0.000 claims description 6
- 239000000047 product Substances 0.000 claims description 6
- 239000002994 raw material Substances 0.000 claims description 6
- 230000002269 spontaneous effect Effects 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
- 235000015099 wheat brans Nutrition 0.000 claims description 6
- 239000002689 soil Substances 0.000 claims description 5
- 238000005520 cutting process Methods 0.000 claims description 4
- 238000002386 leaching Methods 0.000 claims description 4
- 238000012423 maintenance Methods 0.000 claims description 4
- 238000003971 tillage Methods 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 claims description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 2
- 235000013406 prebiotics Nutrition 0.000 claims description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims 4
- 239000002253 acid Substances 0.000 claims 2
- 229910021529 ammonia Inorganic materials 0.000 claims 2
- 244000099147 Ananas comosus Species 0.000 claims 1
- KHBQMWCZKVMBLN-UHFFFAOYSA-N Benzenesulfonamide Chemical compound NS(=O)(=O)C1=CC=CC=C1 KHBQMWCZKVMBLN-UHFFFAOYSA-N 0.000 claims 1
- 244000082204 Phyllostachys viridis Species 0.000 claims 1
- 239000013522 chelant Substances 0.000 claims 1
- 238000012258 culturing Methods 0.000 abstract description 11
- 238000010899 nucleation Methods 0.000 abstract description 5
- 230000036541 health Effects 0.000 abstract description 4
- 239000007787 solid Substances 0.000 abstract description 4
- 239000003814 drug Substances 0.000 abstract description 3
- 241000234671 Ananas Species 0.000 description 8
- 241001330002 Bambuseae Species 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 4
- 230000035784 germination Effects 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- 241000219780 Pueraria Species 0.000 description 2
- 230000010261 cell growth Effects 0.000 description 2
- 235000009508 confectionery Nutrition 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- RMOGWMIKYWRTKW-UONOGXRCSA-N (S,S)-paclobutrazol Chemical compound C([C@@H]([C@@H](O)C(C)(C)C)N1N=CN=C1)C1=CC=C(Cl)C=C1 RMOGWMIKYWRTKW-UONOGXRCSA-N 0.000 description 1
- 241000218645 Cedrus Species 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 239000005985 Paclobutrazol Substances 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 235000021374 legumes Nutrition 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 1
- 230000021332 multicellular organism growth Effects 0.000 description 1
- 239000000618 nitrogen fertilizer Substances 0.000 description 1
- 230000000474 nursing effect Effects 0.000 description 1
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000003375 plant hormone Substances 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 229960004889 salicylic acid Drugs 0.000 description 1
- MCAHWIHFGHIESP-UHFFFAOYSA-N selenous acid Chemical compound O[Se](O)=O MCAHWIHFGHIESP-UHFFFAOYSA-N 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000000811 xylitol Substances 0.000 description 1
- 235000010447 xylitol Nutrition 0.000 description 1
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 description 1
- 229960002675 xylitol Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
- A01G9/0299—Handling or transporting of soil blocks or seedlings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/02—Sulfur; Selenium; Tellurium; Compounds thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/44—Poaceae or Gramineae [Grass family], e.g. bamboo, lemon grass or citronella grass
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
- A61L2/18—Liquid substances or solutions comprising solids or dissolved gases
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C9/00—Fertilisers containing urea or urea compounds
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/23—Solutions
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- Life Sciences & Earth Sciences (AREA)
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- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
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- Biotechnology (AREA)
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- Natural Medicines & Medicinal Plants (AREA)
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- Forests & Forestry (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Developmental Biology & Embryology (AREA)
- Epidemiology (AREA)
- Veterinary Medicine (AREA)
- General Chemical & Material Sciences (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention provides a kind of method of pueraria lobata nursery, it is related to Chinese medicine cultivation technique technical field, the method of pueraria lobata nursery of the invention promotees bud agent stage and nursery stage including preparation, promotees the bud agent stage the following steps are included: strain expansion cultivates, prepares supernatant, preparation chelating liquid, preparation rush bud agent wherein preparing；The nursery stage cultivates kind, the pretreatment of nursery field, cuttage and seedling culture, seedling management the following steps are included: obtaining；Pueraria lobata method for culturing seedlings of the invention is suitable for cuttage seeding and tissue-cultured seedling simultaneously, and solve the problems, such as that now there are two types of method for culturing seedlings, the pueraria lobata seedling produced using pueraria lobata method for culturing seedlings of the invention, seedling body health is sturdy, resistance lays a solid foundation for the high yield of current year, and the seedling raising process, it is convenient, simple to operation, it is environmentally friendly.
Description
[technical field]
The present invention relates to Chinese medicine cultivation technique technical fields, and in particular to a kind of method of pueraria lobata nursery.
[background technique]
Wild Pueraria is the root tuber of legume pueraria lobata or sweet kudzu trailing plants, is a kind of often feeding vegetable of some provinces and regions of southern china
Dish, taste is sweet, cool and tasty, is often used as Baoshang, can also make drug.
Traditional pueraria lobata nursery is cuttage and seedling culture.Generally after harvesting underground root tuber, the potato rattan on top is cut into segment, skewer
Insert nursery.Seedling raise period is generally mid or late January, and or so March next year is lowerly.It is of long duration, and germination percentage is not high.In recent years,
In order to improve yield, aerial growth amount largely is controlled using paclobutrazol, the germination percentage and rooting rate of pueraria lobata cuttage are increasingly
It is low.The germination rooting rate of some peasant households is less than 50%.
Pueraria lobata is other than traditional cuttage seeding, and there are also tissue-cultured seedling.Because tissue-cultured seedling is weaker, breeding and seedling nursing with equipment is needed.Pueraria lobata
The technical difficulty of tissue-cultured seedling nursery is larger, because with catching up under farming season, being typically selected in and starting nursery, normal need or so December
It is 80-90 days or so, at high cost, it is of long duration.
The present invention, which calls on, is studying a kind of pueraria lobata method for culturing seedlings saved production cost, improve yield.
[summary of the invention]
In view of this, the purpose of the present invention is to provide a kind of method of pueraria lobata nursery, pueraria lobata method for culturing seedlings of the invention
It is suitable for cuttage seeding and tissue-cultured seedling simultaneously, and solves the problems, such as that now there are two types of method for culturing seedlings, using Pueraria lobota of the invention
The pueraria lobata seedling of root method for culturing seedlings production, seedling body health is sturdy, resistance, lays a solid foundation for the high yield of current year, and should
Seedling raising process, it is convenient, simple to operation, it is environmentally friendly.
In order to solve the above-mentioned technical problem, present invention employs the following technical solutions:
A kind of method of pueraria lobata nursery, comprising the following steps:
(1) preparation promotees the bud agent stage
(1) strain expands culture: brown sugar and wheat bran being mixed according to the ratio that mass ratio is 1:10-11, access 1-2mL benefit
Raw bacterium, and the water with probiotics equal volume is added, spontaneous fermentation 24-25 hours, it is stand-by to obtain strain；
(2) it prepares supernatant: Pachyrhizua angulatus, pineapple, China fir sawdust being mixed according to the ratio that mass ratio is 1-2:2:1, then
The strain that obtained mixture and step (1) are obtained is mixed according to the ratio that mass ratio is 10-11:1, is put into tank and is sealed hair
Ferment 90-95 days, product after fermentation is filtered, it is stand-by to obtain supernatant；
(3) preparation chelating liquid: a. obtains the selenous acid that concentration is 120-130g/L firstly, sodium selenite is soluble in water
Sodium water solution is that mother liquor is spare；B. the mother liquor of step a is mixed with the amino acid solution that mass concentration is 20-30%, is then existed
Concussion chelating is carried out at 35-45 DEG C 48-72 hours, it is stand-by to obtain chelating liquid；
(4) preparation promotees bud agent: liquid is chelated obtained by supernatant obtained by step (2) and step (3) according to the ratio of volume 3-4:1-2
Example mixing, is subsequently added into sodium alkyl benzene sulfonate；It is stirred at 25-35 DEG C, chelates 36-48 hours, obtain chelating amino acids
Promote bud agent；
(2) the nursery stage
1) it obtains and cultivates kind: taking pueraria lobata rattan, for cutting back at bud point, the short rattan that length is 8-9cm, 1-2cm is stayed on bud point top
7-8cm length is stayed in length, lower part, to avoid the case where overturning end to end, influencing growth occurs；
2) nursery field pre-processes: by field ridging, the high 15-20cm in ridge, and spraying carbendazim disinfection；
3) chelating amino acids obtained in step (1) cuttage and seedling culture: are promoted into bud agent and water according to the volume of 1:600-650
Than dilution, 10-12min then is impregnated in the solution by short rattan that step 1) obtains after dilution, is inserted vertically into ridge after taking-up,
It stays bud point on soil, does not bury all below bud point；
4) seedling management: 6-9 days bud points of cuttage start after sprouting, in step (1) gained chelating amino acids promote bud agent with
Water is primary according to the spray solution that the ratio of 1:1000-1100 is mixed to prepare；To sprouting length to 3-4cm long, and it is true to grow 2-3 piece
Ye Shi carries out spraying for foliar fertilizer in conjunction with tillage and weeding, and to height of seedling 9-11cm, root is flourishing, can carry out field-transplanting.
In the present invention, further, nursery stage of the step (2) the following steps are included:
1. tissue-cultured seedling is handled: after tissue-cultured seedling is cleaned, 9-11min is impregnated with carbendazim, is then transplanted in seedling-raising cup, and
Seedling-raising cup is placed in seedling growth greenhouse；
2. seedling management: to the tissue-cultured seedling water drenching after transplanting, respectively water of leaching, maintenance seedling growth greenhouse temperature be not low sooner or later daily
In 18 DEG C, humidity is not less than 70%, and it is primary to spray bactericide every 6-8 days；The 3-4 days after transplanting, it will be obtained in step (1)
Chelating amino acids promote bud agent and water and diluted according to the volume ratio of 1:950-1000, then by the solution obtained after dilution to tissue culture
Seedling is sprayed, and when growing to sprouting to 2-4cm long, and growing 2-3 piece true leaf, carries out spraying for foliar fertilizer in conjunction with tillage and weeding,
To height of seedling 9-11cm, root is flourishing, can carry out field-transplanting.
In the present invention, further, the volume ratio of mother liquor and amino acid solution is 1:3-4 in the step (3).
In the present invention, further, the additional amount of sodium alkyl benzene sulfonate is to be added by every liter of chelating liquid in the step (4)
5-6g sodium alkyl benzene sulfonate.
In the present invention, further, the foliar fertilizer is made of the raw material of following parts by weight: 20-25 parts of aloe powder, rape
Plain lactones 6-12 parts, 8-16 parts of bamboo vinegar, liquid fertilizer 5-10 parts of sugar alcohol chelating, 10-20 parts of urea and 120-200 parts of water.
In the present invention, further, the step 2. in bactericide be watered dilution 300 times of liquid carbendazim solution.
In conclusion by adopting the above-described technical solution, the beneficial effects of the present invention are:
1. pueraria lobata method for culturing seedlings of the invention is suitable for cuttage seeding and tissue-cultured seedling simultaneously, and solve that existing there are two types of nursery sides
The problem of method, in cuttage seeding cultivation, including preparation promotees bud agent stage and nursery stage, promote bud agent by Pachyrhizua angulatus, pineapple,
Sodium alkyl benzene sulfonate is added after China fir sawdust and the supernatant chelating after strain fermentation to be prepared, wherein Pachyrhizua angulatus provides nitrogen source
And a variety of small-molecule substances, promote cell growth, pineapple provides vitamin, and contains a large amount of reduced sugars and fiber in pineapple
Element, for the need of plant growth, cedar sawdust provides salicylic acid, and three's mixing can effectively facilitate seedling body growth, improve strong sprout rate；
The sodium alkyl benzene sulfonate of addition forms balanced stabilization nutrient unit, guarantees the equilibrium of nutritional ingredient and stablizes；It is used before cuttage
Carbendazim carries out field disinfection, and field itself can be carried to the killing of volume germ, cooperate the management of nursery stage, the pueraria lobata seedling of production,
Seedling body health is sturdy, resistance；Break that seedling raise period existing for existing cuttage and seedling culture is long, and the problem that germination percentage is not high；
In tissue culture, soaking sterilization is carried out to tissue-cultured seedling using carbendazim first, the germ for preventing tissue-cultured seedling from carrying leads to tissue culture
Defect phenomenon is generated in the process, and cooperation sprays bactericide to tissue-cultured seedling, promotees bud agent and foliar fertilizer during culture, is able to solve existing
The problem of tissue culture technology, obtained pueraria lobata seedling seedling body is healthy and strong, and strong plantlets and rootage rate is high, resistance；Nursery of the invention
Method is handled using bud agent is promoted, and can be used not only for cuttage and seedling culture, and be suitable for tissue culture, seedling raise period is than traditional nursery
Time saves about 20 days or so, and pueraria lobata seedling can lowerly ahead of time, and slow seedling, extends growth period ahead of time, for the high yield of current year
It lays a solid foundation；And the seedling raising process, it is convenient, simple to operation, it is environmentally friendly.
2. in foliar fertilizer of the invention of the invention, aloe powder is living to the protective effect of cell tissue and the tax of damaged cell
Palingenesis；Brassin lactones can promote plant growth, and improving quality improves yield, and beautiful in colour, blade is more thick and solid；Sugar
Alcohol chelating liquid fertilizer can be improved the resistance of plant, and main component is exactly sugar alcohol substance, mainly mannitol, sorb
The mixture that sugar alcohol, xylitol and glycerine are formed with nutrient needed for crop in specific proportions, nutritional ingredient can be light
It is easily absorbed by blade, into after blade interior, nutrient can be carried can smoothly enter into and carry out nutrient transport in bast；Urea is
The highest nitrogenous fertilizer of nitrogen content is suitable for various soil and plant, can promote the division and growth of cell, branches and leaves is made to grow in great numbers；
Bamboo vinegar has good permeability and absorbability to the tissue of plant and animal, in terms of plant hormone being played the role of,
Suitable bamboo vinegar is added, then can play and pueraria lobata is promoted to take root, germinate, grow, make pueraria lobata Medicinal Materials Characters more preferably effect.
[specific embodiment]
The following examples can help those skilled in the art that the present invention is more fully understood, but cannot be with any
Mode limits the present invention.
Embodiment 1
A kind of method of pueraria lobata nursery provided by the present embodiment the following steps are included:
(1) strain expands culture: brown sugar and wheat bran are mixed according to the ratio that mass ratio is 1:10, access 1mL probiotics,
And the water with probiotics equal volume is added, spontaneous fermentation 24 hours, it is stand-by to obtain strain；
(2) it prepares supernatant: Pachyrhizua angulatus, pineapple, China fir sawdust being mixed according to the ratio that mass ratio is 1:2:1, then will
The strain that obtained mixture and step (1) obtains is mixed according to the ratio that mass ratio is 10:1, is put into tank and is sealed by fermentation 90
It, product after fermentation is filtered, it is stand-by to obtain supernatant；
(3) preparation chelating liquid: a. obtains the sodium selenite water that concentration is 120g/L firstly, sodium selenite is soluble in water
Solution is that mother liquor is spare；B. the mother liquor of step a is mixed with the amino acid solution that mass concentration is 20%, mother liquor and amino acid
The volume ratio of solution is 1:3；Then concussion chelating is carried out at 35 DEG C 48 hours, it is stand-by to obtain chelating liquid；
(4) preparation promotees bud agent: chelating liquid obtained by supernatant obtained by step (2) and step (3) is mixed according to the ratio of volume 3:1
It closes, is subsequently added into sodium alkyl benzene sulfonate, the additional amount of sodium alkyl benzene sulfonate is that 5g sodium alkyl benzene sulfonate is added by every liter of chelating liquid；
It is stirred, chelates 36 hours at 25 DEG C, obtain chelating amino acids and promote bud agent；
(2) the nursery stage
1) it obtaining and cultivates kind: taking pueraria lobata rattan, for cutting back at bud point, the short rattan that length is 8cm, 1cm length is stayed on bud point top,
7cm length is stayed in lower part, to avoid the case where overturning end to end, influencing growth occurs；
2) nursery field pre-processes: by field ridging, the high 15cm in ridge, and spraying carbendazim disinfection；
3) cuttage and seedling culture: chelating amino acids obtained in step (1) are promoted into bud agent and water is dilute according to the volume ratio of 1:600
It releases, then impregnates 10min in the solution by short rattan that step 1) obtains after dilution, be inserted vertically into ridge after taking-up, stay bud point
In not burying all on soil, below bud point；
4) seedling management: 6 days bud points of cuttage start after sprouting, and promote bud agent and water with gained chelating amino acids in step (1)
The spray solution being mixed to prepare according to the ratio of 1:1000 is primary；When growing to sprouting to 3cm long, and growing 2 true leaves, in conjunction in
Weeding carries out foliar fertilizer and sprays, and foliar fertilizer is made of the raw material of following parts by weight: 20 parts of aloe powder, 6 parts of brassin lactones,
8 parts of bamboo vinegar, 5 parts of liquid fertilizer of sugar alcohol chelating, 10 parts of urea and 120 parts of water；To height of seedling 9cm, root is flourishing, can carry out big
Field transplanting.
Embodiment 2
A kind of method of pueraria lobata nursery provided by the present embodiment the following steps are included:
(1) strain expands culture: brown sugar and wheat bran being mixed according to the ratio that mass ratio is 1:11, access 1-2mL is prebiotic
Bacterium, and the water with probiotics equal volume is added, spontaneous fermentation 25 hours, it is stand-by to obtain strain；
(2) it prepares supernatant: Pachyrhizua angulatus, pineapple, China fir sawdust being mixed according to the ratio that mass ratio is 2:2:1, then will
The strain that obtained mixture and step (1) obtains is mixed according to the ratio that mass ratio is 11:1, is put into tank and is sealed by fermentation 95
It, product after fermentation is filtered, it is stand-by to obtain supernatant；
(3) preparation chelating liquid: a. obtains the sodium selenite water that concentration is 130g/L firstly, sodium selenite is soluble in water
Solution is that mother liquor is spare；B. the mother liquor of step a is mixed with the amino acid solution that mass concentration is 30%, mother liquor and amino acid
The volume ratio of solution is 1:4；Then concussion chelating is carried out at 45 DEG C 72 hours, it is stand-by to obtain chelating liquid；
(4) preparation promotees bud agent: chelating liquid obtained by supernatant obtained by step (2) and step (3) is mixed according to the ratio of volume 2:1
It closes, is subsequently added into sodium alkyl benzene sulfonate, the additional amount of sodium alkyl benzene sulfonate is that 6g sodium alkyl benzene sulfonate is added by every liter of chelating liquid；
It is stirred, chelates 48 hours at 35 DEG C, obtain chelating amino acids and promote bud agent；
(2) the nursery stage
1) it obtaining and cultivates kind: taking pueraria lobata rattan, for cutting back at bud point, the short rattan that length is 9cm, 2cm length is stayed on bud point top,
7cm length is stayed in lower part, to avoid the case where overturning end to end, influencing growth occurs；
2) nursery field pre-processes: by field ridging, the high 20cm in ridge, and spraying carbendazim disinfection；
3) cuttage and seedling culture: chelating amino acids obtained in step (1) are promoted into bud agent and water is dilute according to the volume ratio of 1:650
It releases, then impregnates 12min in the solution by short rattan that step 1) obtains after dilution, be inserted vertically into ridge after taking-up, stay bud point
In not burying all on soil, below bud point；
4) seedling management: 9 days bud points of cuttage start after sprouting, and promote bud agent and water with gained chelating amino acids in step (1)
The spray solution being mixed to prepare according to the ratio of 1:1100 is primary；When growing to sprouting to 4cm long, and growing 3 true leaves, in conjunction in
Weeding carries out spraying for foliar fertilizer, and foliar fertilizer is made of the raw material of following parts by weight: 25 parts of aloe powder, brassin lactones 12
Part, 10 parts of liquid fertilizer of 16 parts of bamboo vinegar, sugar alcohol chelating, 20 parts of urea and 200 parts of water；To height of seedling 11cm, root is flourishing
Carry out field-transplanting.
Embodiment 3
A kind of method of pueraria lobata nursery provided by the present embodiment the following steps are included:
(1) strain expands culture: brown sugar and wheat bran are mixed according to the ratio that mass ratio is 1:10, access 1mL probiotics,
And the water with probiotics equal volume is added, spontaneous fermentation 24 hours, it is stand-by to obtain strain；
(2) it prepares supernatant: Pachyrhizua angulatus, pineapple, China fir sawdust being mixed according to the ratio that mass ratio is 1:2:1, then will
The strain that obtained mixture and step (1) obtains is mixed according to the ratio that mass ratio is 10:1, is put into tank and is sealed by fermentation 90
It, product after fermentation is filtered, it is stand-by to obtain supernatant；
(3) preparation chelating liquid: a. obtains the sodium selenite water that concentration is 120g/L firstly, sodium selenite is soluble in water
Solution is that mother liquor is spare；B. the mother liquor of step a is mixed with the amino acid solution that mass concentration is 20%, mother liquor and amino acid
The volume ratio of solution is 1:3；Then concussion chelating is carried out at 35 DEG C 48 hours, it is stand-by to obtain chelating liquid；
(4) preparation promotees bud agent: chelating liquid obtained by supernatant obtained by step (2) and step (3) is mixed according to the ratio of volume 3:1
It closes, is subsequently added into sodium alkyl benzene sulfonate, the additional amount of sodium alkyl benzene sulfonate is that 5g sodium alkyl benzene sulfonate is added by every liter of chelating liquid；
It is stirred, chelates 36 hours at 25 DEG C, obtain chelating amino acids and promote bud agent；
(2) the nursery stage
1. tissue-cultured seedling is handled: after tissue-cultured seedling is cleaned, impregnating 9min with carbendazim, be then transplanted in seedling-raising cup, and will
Seedling-raising cup is placed in seedling growth greenhouse；
2. seedling management: to the tissue-cultured seedling water drenching after transplanting, respectively water of leaching, maintenance seedling growth greenhouse temperature be not low sooner or later daily
In 18 DEG C, humidity is not less than 70%, sprays that bactericide is primary every 6 days, bactericide be watered 300 times of liquid of dilution carbendazim it is molten
Liquid；The 3rd day after transplanting, chelating amino acids obtained in step (1) are promoted into bud agent and water and are diluted according to the volume ratio of 1:950, so
The solution obtained after dilution sprays tissue-cultured seedling afterwards, when growing to sprouting to 2cm long, and growing 2 true leaves, in conjunction with intertillage
Weeding carries out spraying for foliar fertilizer, and foliar fertilizer is made of the raw material of following parts by weight: 20 parts of aloe powder, 6 parts of brassin lactones, bamboo
8 parts of vinegar liquid, 5 parts of liquid fertilizer of sugar alcohol chelating, 10 parts of urea and 120 parts of water；To height of seedling 9cm, root is flourishing, can carry out crop field
Transplanting.
Embodiment 4
A kind of method of pueraria lobata nursery provided by the present embodiment the following steps are included:
(1) strain expands culture: brown sugar and wheat bran are mixed according to the ratio that mass ratio is 1:11, access 2mL probiotics,
And the water with probiotics equal volume is added, spontaneous fermentation 25 hours, it is stand-by to obtain strain；
(2) it prepares supernatant: Pachyrhizua angulatus, pineapple, China fir sawdust being mixed according to the ratio that mass ratio is 2:2:1, then will
The strain that obtained mixture and step (1) obtains is mixed according to the ratio that mass ratio is 11:1, is put into tank and is sealed by fermentation 95
It, product after fermentation is filtered, it is stand-by to obtain supernatant；
(3) preparation chelating liquid: a. obtains the sodium selenite water that concentration is 130g/L firstly, sodium selenite is soluble in water
Solution is that mother liquor is spare；B. the mother liquor of step a is mixed with the amino acid solution that mass concentration is 30%, mother liquor and amino acid
The volume ratio of solution is 1:4；Then concussion chelating is carried out at 45 DEG C 72 hours, it is stand-by to obtain chelating liquid；
(4) preparation promotees bud agent: chelating liquid obtained by supernatant obtained by step (2) and step (3) is mixed according to the ratio of volume 2:1
It closes, is subsequently added into sodium alkyl benzene sulfonate, the additional amount of sodium alkyl benzene sulfonate is that 6g sodium alkyl benzene sulfonate is added by every liter of chelating liquid；
It is stirred, chelates 48 hours at 35 DEG C, obtain chelating amino acids and promote bud agent；
(2) the nursery stage
1. tissue-cultured seedling is handled: after tissue-cultured seedling is cleaned, impregnating 11min with carbendazim, be then transplanted in seedling-raising cup, and will
Seedling-raising cup is placed in seedling growth greenhouse；
2. seedling management: to the tissue-cultured seedling water drenching after transplanting, respectively water of leaching, maintenance seedling growth greenhouse temperature be not low sooner or later daily
In 18 DEG C, humidity is not less than 70%, sprays that bactericide is primary every 8 days, bactericide be watered 300 times of liquid of dilution carbendazim it is molten
Liquid；The 4th day after transplantings, chelating amino acids obtained in step (1) are promoted into bud agent and water and are diluted according to the volume ratio of 1:1000,
Then the solution obtained after dilution sprays tissue-cultured seedling, when growing to sprouting to 4cm long, and growing 3 true leaves, in conjunction in
Weeding carries out spraying for foliar fertilizer, and foliar fertilizer is made of the raw material of following parts by weight: 25 parts of aloe powder, brassin lactones 12
Part, 10 parts of liquid fertilizer of 16 parts of bamboo vinegar, sugar alcohol chelating, 20 parts of urea and 200 parts of water；To height of seedling 11cm, root is flourishing
Carry out field-transplanting.
Compliance test result
The practical value of the method for pueraria lobata nursery in order to further illustrate the present invention, applicant do comparative test:
First group: pueraria lobata method for culturing seedlings described in the embodiment of the present invention 1；
Second group: pueraria lobata method for culturing seedlings described in the embodiment of the present invention 3；
Third group: traditional pueraria lobata cuttage and seedling culture method；
4th group: traditional pueraria lobata tissue culture method.
S1: the case where observation cultivates pueraria lobata by above-mentioned four groups of method for culturing seedlings, and nursery situation is recorded as shown in table 1:
1 nursery situation contrast table of table
| Group | Rooting rate (%) | More rates (%) | Survival rate (%) | Seedling raise period (day) |
| First group | 95 | 56 | 96 | 92 |
| Second group | 96 | 54 | 95 | 90 |
| Third group | 80 | 29 | 73 | 113 |
| 4th group | 82 | 27 | 75 | 110 |
S2: carrying out the observation in transplanting crop field, and the management after four groups of transplanting processes and transplanting is identical, observes above-mentioned pueraria lobata seedling
In the growing state of transplanting crop field after two months.Transplanting survival rate is counted, and result is recorded as shown in table 2:
2 four groups of pueraria lobata transplanted seedling growing state tables of table
Learnt by above-mentioned two table: the pueraria lobata cultivated using cultivating method of the invention, seedling body health is sturdy, resistance,
The seedling of high-quality not only is obtained in the seedling culture stage, and is also able to maintain good growing way, each step after transplanting crop field
It combines closely, interacts, improve the survival rate of pueraria lobata, be suitable for large-scale plantation, be worthy to be popularized.
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements, fall within the scope of the claimed invention without departing from theon the basis of the spirit of the present invention.
Claims (6)
1. a kind of method of pueraria lobata nursery, which is characterized in that the method for the pueraria lobata nursery the following steps are included:
(1) preparation promotees the bud agent stage
(1) strain expands culture: brown sugar and wheat bran being mixed according to the ratio that mass ratio is 1:10-11, access 1-2mL is prebiotic
Bacterium, and the water with probiotics equal volume is added, spontaneous fermentation 24-25 hours, it is stand-by to obtain strain；
(2) it prepares supernatant: Pachyrhizua angulatus, pineapple, China fir sawdust being mixed according to the ratio that mass ratio is 1-2:2:1, then incited somebody to action
To the obtained strain of mixture and step (1) mixed according to the ratio that mass ratio is 10-11:1, be put into tank and be sealed by fermentation
90-95 days, product after fermentation is filtered, it is stand-by to obtain supernatant；
(3) preparation chelating liquid: a. obtains the sodium selenite water that concentration is 120-130g/L firstly, sodium selenite is soluble in water
Solution is that mother liquor is spare；B. the mother liquor of step a is mixed with the amino acid solution that mass concentration is 20-30%, then in 35-
Concussion chelating is carried out at 45 DEG C 48-72 hours, it is stand-by to obtain chelating liquid；
(4) preparation promotees bud agent: chelating liquid obtained by supernatant obtained by step (2) and step (3) is mixed according to the ratio of volume 3-4:1-2
It closes, is subsequently added into sodium alkyl benzene sulfonate；It is stirred, chelates 36-48 hours at 25-35 DEG C, obtain chelating amino acids and promote bud
Agent；
(2) the nursery stage
1) it obtaining and cultivates kind: taking pueraria lobata rattan, for cutting back at bud point, the short rattan that length is 8-9cm, 1-2cm length is stayed on bud point top,
7-8cm length is stayed in lower part, to avoid the case where overturning end to end, influencing growth occurs；
2) nursery field pre-processes: by field ridging, the high 15-20cm in ridge, and spraying carbendazim disinfection；
3) cuttage and seedling culture: chelating amino acids obtained in step (1) are promoted into bud agent and water is dilute according to the volume ratio of 1:600-650
It releases, then impregnates 10-12min in the solution by short rattan that step 1) obtains after dilution, be inserted vertically into ridge after taking-up, stay bud
During point does not bury all on soil, below bud point；
4) seedling management: 6-9 days bud points of cuttage start after sprouting, and promote bud agent with gained chelating amino acids in step (1) and press with water
The spray solution being mixed to prepare according to the ratio of 1:1000-1100 is primary；To sprouting length to 3-4cm long, and grow 2-3 piece true leaf
When, spraying for foliar fertilizer is carried out in conjunction with tillage and weeding, to height of seedling 9-11cm, root is flourishing, can carry out field-transplanting.
2. a kind of method of pueraria lobata nursery according to claim 1, which is characterized in that the nursery stage of the step (2)
The following steps are included:
1. tissue-cultured seedling is handled: after tissue-cultured seedling is cleaned, impregnating 9-11min with carbendazim, be then transplanted in seedling-raising cup, and will educate
Seedling cup is placed in seedling growth greenhouse；
2. seedling management: to the tissue-cultured seedling water drenching after transplanting, respectively water of leaching, maintenance seedling growth greenhouse temperature are not less than 18 sooner or later daily
DEG C, humidity is not less than 70%, and it is primary to spray bactericide every 6-8 days；The 3-4 days after transplanting, by ammonia obtained in step (1)
Base acid chelating promotees bud agent and water and dilutes according to the volume ratio of 1:950-1000, then by the solution obtained after dilution to tissue-cultured seedling into
Capable sprinkling when growing to sprouting to 2-4cm long, and growing 2-3 piece true leaf, carries out spraying for foliar fertilizer in conjunction with tillage and weeding, to seedling
High 9-11cm, root is flourishing, can carry out field-transplanting.
3. a kind of method of pueraria lobata nursery according to claim 1, which is characterized in that mother liquor and ammonia in the step (3)
The volume ratio of base acid solution is 1:3-4.
4. a kind of method of pueraria lobata nursery according to claim 1, which is characterized in that benzene sulfonamide in the step (4)
The additional amount of sour sodium is that 5-6g sodium alkyl benzene sulfonate is added by every liter of chelating liquid.
5. according to claim 1 or 2 a kind of described in any item methods of pueraria lobata nursery, which is characterized in that the foliar fertilizer by
The raw material of following parts by weight forms: 20-25 parts of aloe powder, 6-12 parts of brassin lactones, 8-16 parts of bamboo vinegar, sugar alcohol chelate liquid
5-10 parts of fertilizer, 10-20 parts of urea and 120-200 parts of water.
6. a kind of method of pueraria lobata nursery according to claim 2, which is characterized in that the step 2. in bactericide be
It is watered the carbendazim solution of 300 times of liquid of dilution.
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| CN111153745A (en) * | 2020-03-04 | 2020-05-15 | 广西壮族自治区农业科学院 | Wild pueraria lobata rooting culture solution and wild pueraria lobata rooting culture method |
| CN112262721A (en) * | 2020-10-30 | 2021-01-26 | 安徽世纪绿药生态农林有限公司 | Selenium-rich radix puerariae planting method |
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