CN105900741A - Method for cultivating pachyrhizua angulatus tissue culture seedlings by means of plant growth lamps - Google Patents
Method for cultivating pachyrhizua angulatus tissue culture seedlings by means of plant growth lamps Download PDFInfo
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- CN105900741A CN105900741A CN201610292172.XA CN201610292172A CN105900741A CN 105900741 A CN105900741 A CN 105900741A CN 201610292172 A CN201610292172 A CN 201610292172A CN 105900741 A CN105900741 A CN 105900741A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/14—Greenhouses
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
- A01G7/045—Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/25—Greenhouse technology, e.g. cooling systems therefor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/14—Measures for saving energy, e.g. in green houses
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Abstract
The invention provides a method for cultivating pachyrhizua angulatus tissue culture seedlings by means of plant growth lamps. The method comprises the following steps of putting up a greenhouse, determining and customizing the plant growth lights, selecting a seedling-raising substrate, selecting pachyrhizua angulatus tissue culture seedlings, raising the seedlings and conducting seedling-raising management. The greenhouse adopts an ordinary steel frame structure, one side of the greenhouse is provided with a rolling gate used for being rolled up or laid down, and therefore the temperature in the greenhouse is adjusted; each plant growth lamp adopts an LED of which the lamp head power is 3 W, and a 220 v alternating current power supply is adopted to serve as a luminous source, wherein the ratio of red-light lamp heads to blue-light lamp heads is 7:2; the substrate is prepared from clean loess, yellow sand and organic fertilizer, the ratio of clean loess to yellow sand to organic fertilizer is 1 to 1 to 0.2, and the organic fertilizer is prepared by evenly blending and fermenting sheep manure, chicken manure, peanut bran and tea bran according to the proportion of 1:1:0.5:0.2. The survival rate of the pachyrhizua angulatus tissue culture seedlings cultivated through the method is high, and compared with the situation that seedling raising can be conducted only in February and March in the prior art, by means of the method, seedling raising can be advanced to be conducted from December of previous year, the peak that kudzuvine rhizomes appear on the market in a centralized mode is avoided, the economic benefit is improved, and the time cost is reduced.
Description
Technical field
Present invention industry concerning farmers crop plants tissue cultivating technical field, especially one utilize plant growth lamp to cultivate Pachyrhizua angulatus tissue cultured seedling
Method.
Background technology
Pachyrhizua angulatus, the another name of Radix Puerariae, also known as Radix puerariae, Radix Puerariae, for the tuber of pulse family perennial liana Pueraria lobota, because of containing volume starch
(in fresh Radix Puerariae, content is 19～20%), therefore named.Herba Gelsemii Elegantis is that yield is high, uses wide kind；And Pachyrhizua angulatus with artificial culture is
Main.Eating Pachyrhizua angulatus and our health is had a lot of benefit, Pachyrhizua angulatus not only has yang invigorating expelling pathogenic factors from muscles, work is quenched the thirst in rash antidiarrheal, relieving restlessness
With, and there is effect of heat-clearing and toxic substances removing, Pachyrhizua angulatus also has the effect alleviating headache simultaneously, usually eats Pachyrhizua angulatus day and can also protect by let us
Hold the quiet of mood, have a healthy good sleep；It is used as medicine and treats hypertension, angina pectoris, gastric ulcer, stomach spasm, gastritis
The diseases such as property pain；It is parts of generic medicinal plants, is again meals good merchantable brands.Pachyrhizua angulatus can be processed into Amylum Puerariae Radicis, Pueraria lobota sheet is refined into graininess speed
Molten Amylum Puerariae Radicis, kudzu root tea also can extract puerarin etc..
So, the cultivation of Pachyrhizua angulatus tissue cultured seedling has important research and is worth.Pachyrhizua angulatus grows by composite factors such as humidity, temperature, illumination
Impact, produce at present, Pachyrhizua angulatus tissue cultured seedling seedling-raising technique mainly uses simple plastic greenhouse seedling raising manners, nursery work typically 2,
March carries out, and northern area is more late.Simple plastic greenhouse seedling-raising technique mainly uses scaffolding, improves temperature of shed, uses light educating
Seedling substrate or sandy soil are cultivation way, cultivate tissue cultured seedling.Coordinate and certain water, apply fertilizer, cultivate suitable field production
Seedling.For the problem that Pachyrhizua angulatus tissue cultured seedling survival rate is low, also someone have employed daylight lamp light filling or fluorescent lamp light filling, effect base
Can't see on Ben.The trophophase of Pachyrhizua angulatus is 10～December, produces at present and is limited by seedling-growing time, typically mid-April with
Rear just start transplant plantation, to Second Year 1, February results concentrate listing, and it is demonstrated experimentally that Pachyrhizua angulatus main product ground, 3,4,
The weather conditions in May are that Pachyrhizua angulatus is nourished and grown vigorous period, and therefore, seedling raise period carried to February, can be Pachyrhizua angulatus
Land for growing field crops high yield is taken a firm foundation, and saves time cost, obtains Pachyrhizua angulatus finished product ahead of time, listing peak of staggering；But, February
In the past, owing to weather is sombre, the reasons such as temperature is low, illuminance is inadequate, cause Pachyrhizua angulatus tissue cultured seedling to cultivate weak effect, quality is low,
Being mainly manifested in survival rate of seedling low, the seedling survived is affected because of the factor inadequate by temperature and effective illuminance, occur stem stalk thin,
Excessive growth, transplants the problems such as resistance is low, also occurs in that the method that the survival rate into improving tissue cultured seedling improves temperature of shed, knot in production
Fruit is higher because of humiture, causes substantial amounts of tissue cultured seedling fungal infection phenomenon, by spraying the medicines such as carbendazim, is the most also difficult to press down
System.Directly affect time and the quality of field-transplanting, improve production cost.
The present invention is to solve shortcoming of the prior art, it is provided that a kind of new Pachyrhizua angulatus tissue cultured seedling breeding method, effectively solve
Carried out Pachyrhizua angulatus tissue cultured seedling before annual February to cultivate shoot survival percent illuminance low, effective inadequate in the past, occurs that stem stalk is thin, excessive growth,
The problem transplanting the low grade of resistance.
Summary of the invention
For the variety of problems faced described in background technology, it is an object of the invention to provide one and utilize plant growth lamp to cultivate powder
The method of Pueraria lobota tissue cultured seedling.For reaching above-mentioned purpose, the technical solution adopted in the present invention is:
A kind of method utilizing plant growth lamp to cultivate Pachyrhizua angulatus tissue cultured seedling of the present invention, comprises the steps:
Building booth: build the booth of steel frame construction, the side of described booth is provided with shutter door, is used for rolling or putting down, and uses
To regulate temperature of shed so that temperature of shed is stable between 20-25 DEG C；Booth shoulder height 1.4-1.7 rice, facilitates personnel to carry out
Management.
Further, it is determined that the quantity of plant growth lamp customize plant growth lamp: determine plant according to the area of set up booth
The quantity of growth lamp.By calculating, the effective range of customized each plant growth lamp is 3.5 squares.Customized plant growing
Lamp: the effective range of customized every unit plant growth lamp illumination is 3.5 square metres, customized plant growth lamp uses single lamp holder
Power is 3W, uses the LED etc. of 220v alternating current power supply as luminous source, and the quantity of described every unit design lamp holder is 55,
Red 43, lamp holder in described lamp holder, blue 12, lamp holder, described red lamp holder is 7:2 with blue light lamp holder ratio, according to 3
The ratio that individual red lamp holder connects a blue lamp holder is installed, and described red lamp holder is used for connecting red light, and described blue lamp holder is used for
Connect blue lamp；Plant growth lamp shape uses box structure, built-in fans fin.
Further, seedling medium selects: select clean loess, yellow sand, fertilizer as seedling medium；Described fertilizer
For sheep stool, chicken manure, peanut residue, tea bran；Described substrate mix homogeneously is stand-by；Loess in seedling medium, yellow sand, fertilizer
Ratio is 1:1:0.2；Sheep stool in described fertilizer, chicken manure, peanut residue, tea bran mix according to the ratio of 1:1:0.5:0.2
Even fermentation.Because the content of organic matter is low in substrate, temperature of shed is relatively low, can be prevented effectively from the situation of fungal infection.
Further, select Pachyrhizua angulatus tissue cultured seedling: Pachyrhizua angulatus tissue cultured seedling is placed in the water of 19-21 DEG C cleaning, culture medium is cleaned dry
Only；The tissue cultured seedling hymexazol cleaned up being soaked, the time that described hymexazol is soaked is 4-5min；Group training after soaking
Seedling is placed in the place of shady and cool ventilation and carries out seedling exercising, it is to avoid blowing or the sun directly shine.
Further, selection basal diameter is 8cm, the round plastic nutrient cup of a height of 9cm carries out nursery, by obtained above
The seedling medium of mix homogeneously loads in each nutrient cup, described seedling medium pave after from nutrient cup rim of a cup 1-2cm.
Further, the Pachyrhizua angulatus tissue cultured seedling of above-mentioned select is implanted in described each nutrient cup installing substrate respectively.Nutrient cup
Arrangement press array type square arrangement, nutrient cup is centered by the wick of each described plant growth lamp, with 3.5 meters of scopes of surrounding
Inside being limited, a point row is marked each nutrient cup by numeral；The nutrient cup of seedlings cultivating carries out position with the nutrient cup away from wick tissue cultured seedling
Put exchange.Near wick the Pachyrhizua angulatus tissue cultured seedling speed of growth than away from the Pachyrhizua angulatus tissue cultured seedling of wick group high or sturdy luxuriant time, will
The withered Pachyrhizua angulatus tissue cultured seedling of or slice short away from plant lamp growing way and the high or sturdy luxuriant Pachyrhizua angulatus near plant growth lamp growing way
Tissue cultured seedling reversing of position, reaches to grow consistent purpose.Be conducive to exchanging according to different growing states at different times, lean on
The tissue cultured seedling growth at low beam center can be significantly better than periphery, and the most cup type cultivation can be exchanged according to growing state, reached
Growth is consistent.
Further, nursery prophase management: after tissue cultured seedling is implanted nutrient cup 14-16 days is nursery early stage；Need every day sooner or later
Each trickle once, makes humidity 18-30 DEG C in canopy, controls humidity and is once watered 300 times of liquid of dilution in 70%-90%, spray in every 7 days
Carbendazim, and determine illumination duration according to rain or shine weather: at rainy weather, the illumination in 24 hours of plant growth lamp whole day；Fine
My god, every day, lighting time controlled at 16-17 hour.
Further, nursery middle term management: nursery is after nursery early stage 10-12 days mid-terms, when tissue cultured seedling, substantially to enter growth prosperous
In the Sheng stage, leaf growth is obvious, and root system stretches.8 o'clock every mornings needed trickle once by 9 o'clock, opened booth simultaneously, ventilated
Ventilation, also functions to seedling exercising effect.Plant growth lamp lighting time: about 14 hours every day of fine day, overcast and rainy every day 16-18
Individual hour.
Further, nursery final-period management: whole day opens booth, make ventilation in booth, on the one hand play seedling exercising effect,
On the other hand reducing humidity prevents the fungal diseases such as powdery mildew, downy mildew, mycete from occurring；Run into Pachyrhizua angulatus tissue cultured seedling seedling-raising cup soil
Dry turning white is accomplished by watering at once, and occurring without Pachyrhizua angulatus tissue cultured seedling seedling-raising cup soil, dry 1-2 days trickles are once when turning white；Plant lamp
Every day, lighting time controlled at 10-12 hour.
Further, transplant in good time, after Pachyrhizua angulatus Seedling enters vigorous period, after the speed of growth grows up to seedling the soonest, run into
Weather temperature is higher than 35 DEG C or weather temperature is less than 10 DEG C or the most overcast and rainy unfavorable conditions, and now, land for growing field crops is not suitable for transplanting,
Plant growth lamp lighting time described above is brought up to 24 hours, illumination full sun, to reach temporarily to suppress Pachyrhizua angulatus Seedling excessive growth
Purpose；Deng stable when 20 DEG C-30 DEG C to land for growing field crops climatic environment, now, land for growing field crops is suitable for transplanting, and can transplant immediately.
The method have the advantages that
1, can overcome in prior art and have to be advanced to previous year to Pachyrhizua angulatus tissue cultured seedling cultivation work just can be carried out after March
December is cultivated, and overcomes that December is sombre to weather in February in the coming year, temperature is low, illuminance makes Pachyrhizua angulatus cultivate seedling to survive not
The technological difficulties that rate is low.
2, utilize nutrient cup to carry out cultivating and respectively nutrient cup being marked centered by plant growth lamp wick, be conducive to not
Same time is exchanged according to different growing states, and the tissue cultured seedling growth near lamp center can be significantly better than periphery, the most cup type cultivation
Training can be exchanged according to growing state, reaches growth consistent.
3, in the case of need not promote temperature of shed, effective illumination is increased, the survival rate of tissue cultured seedling, and rate contrast in strong sprout
Former years are significantly improved.Do not heat up, greatly suppress the infection of mycete.
4, nursery after March, cultivates the time too late, and Pachyrhizua angulatus is short at big field growth time, does not reaches normal growth time,
Potato can not be tied then or yield is the lowest.
Accompanying drawing explanation
Fig. 1 is plant growth lamp unit layout viewing
Description of reference numerals:
1 circle represents red lamp holder, and 2 trianglees represent blue lamp holder
Detailed description of the invention
Make furtherly below in conjunction with the different embodiments method utilizing plant growth lamp to cultivate Pachyrhizua angulatus tissue cultured seedling a kind of to the present invention
Bright.
Embodiment one
Shown in Fig. 1, Fig. 1 is the layout viewing of every unit plant growth lamp, utilizes the plant growth lamp of customization to carry out Pachyrhizua angulatus tissue cultured seedling
Cultivating, cultivating the working time selects at December-during February next year, comprise the following steps:
(1) building booth: build the booth of steel frame construction, the side of booth is provided with shutter door, is used for rolling or putting down, in order to adjust
Joint temperature of shed so that temperature of shed is stable between 20-25 DEG C；Booth shoulder height 1.4-1.7 rice, facilitates personnel to be managed.
(2) customized plant growth lamp: determine the quantity of plant growth lamp according to the area of set up booth.By calculating, customized is every
The effective range of unit plant growth lamp illumination is 3.5 square metres, and customized plant growth lamp uses single lamp holder power to be 3W, adopt
LED by 220v alternating current power supply etc., as luminous source, are 55 in conjunction with the quantity of every unit design lamp holder described in Fig. 1, wherein
Red 43, lamp holder, blue 12, lamp holder；Described red lamp holder quantity is 7:2 with blue-ray light lamp holder quantitative proportion, such as Fig. 1
The shown ratio installation connecting blue lamp holders (2) according to 3 red lamp holders (1)；Described red lamp holder is used for connecting red
Lamp, described blue lamp holder is used for connecting blue lamp；Every unit plant growth lamp shape uses box structure, built-in fans fin.
(3) seedling medium selects: select clean loess, yellow sand, fertilizer as seedling medium；Described fertilizer is sheep stool, chicken
Excrement, peanut residue, tea bran；Described substrate mix homogeneously is stand-by；Loess in seedling medium, yellow sand, fertilizer ratio are 1:1:
0.2；Sheep stool in described fertilizer, chicken manure, peanut residue, tea bran mix fermentation according to the ratio of 1:1:0.5:0.2.Because
In substrate, the content of organic matter is low, and temperature of shed is relatively low, can be prevented effectively from the situation of fungal infection.
(4) Pachyrhizua angulatus tissue cultured seedling is selected: Pachyrhizua angulatus tissue cultured seedling is placed in the water of 19-21 DEG C cleaning, culture medium is cleaned up；To clean
Clean tissue cultured seedling hymexazol is soaked, and the time that described hymexazol is soaked is 4-5min；Tissue cultured seedling after soaking is placed in cool place
The place ventilated carries out seedling exercising, it is to avoid blowing or the sun directly shine.
(5) nursery: select basal diameter, the typical circular plastic nutritional cup of a height of 8cm*9cm to carry out nursery, by obtained above mixed
Close uniform seedling medium to load in each nutrient cup, described seedling medium pave after from nutrient cup rim of a cup 1-2cm.Choose above-mentioned
The Pachyrhizua angulatus tissue cultured seedling chosen is implanted in described each nutrient cup installing substrate respectively.Array type square arrangement is pressed in the arrangement of nutrient cup,
Described nutrient cup is centered by the wick of each described plant growth lamp, and to be limited in the range of surrounding 3.5 meters, a point row enters by numeral
The each nutrient cup of line flag.Higher or sturdy than the Pachyrhizua angulatus tissue cultured seedling away from wick group near the Pachyrhizua angulatus tissue cultured seedling speed of growth of wick
Time luxuriant, will be far from the Pachyrhizua angulatus tissue cultured seedling that plant lamp growing way is short or slice is withered high or sturdy with near plant growth lamp growing way
Luxuriant Pachyrhizua angulatus tissue cultured seedling reversing of position, reaches to grow consistent purpose.
(6) nursery prophase management: after tissue cultured seedling is implanted nutrient cup 14-16 days is nursery early stage；Need the every day of each trickle sooner or later once,
Make humidity 18-30 DEG C in canopy, control humidity and be once watered the carbendazim of 300 times of liquid of dilution in 70%-90%, spray in every 7 days, and
Illumination duration is determined: in rainy weather, the illumination in 24 hours of plant growth lamp whole day according to rain or shine weather；At fine day, every day turns on light
Time controlled at 16-17 hour.
(7) nursery middle term management: nursery is after nursery early stage 10-12 days mid-terms, when tissue cultured seedling substantially enters the growth vigorous stage,
Leaf growth is obvious, and root system stretches.8 o'clock every mornings needed trickle once by 9 o'clock, opened booth simultaneously, ventilation, also
Play seedling exercising effect.Plant growth lamp lighting time: about 14 hours every day of fine day, 16-18 hour overcast and rainy every day.
(8) nursery final-period management: whole day opens booth, makes ventilation in booth, on the one hand plays seedling exercising effect, on the other hand fall
Low humidity prevents the fungal diseases such as powdery mildew, downy mildew, mycete from occurring；Run into Pachyrhizua angulatus tissue cultured seedling seedling-raising cup soil dry just to turn white
Needing to water at once, occurring without Pachyrhizua angulatus tissue cultured seedling seedling-raising cup soil, dry 1-2 days trickles are once when turning white；When plant lamp is turned on light every day
Between control at 10-12 hour.Plant lamp lighting time every day controlled at 10-12 hour.
(9) transplant: the Pachyrhizua angulatus seedling obtained through step (8), transplant immediately when running into applicable field-transplanting environment；I.e.
Higher than 35 DEG C or weather temperature is less than 10 DEG C or the most overcast and rainy unfavorable conditions to run into weather temperature, and land for growing field crops is not suitable for transplanting,
Plant growth lamp lighting time described above is brought up to 24 hours；Deng stable when 20 DEG C-30 DEG C to land for growing field crops climatic environment,
Transplant immediately.
In order to further illustrate advantages of the present invention, further illustrating result below by three groups of contrast experiments, often group contrast experiment is equal
As a example by 1000 strain seedling:
Experimental group one
Experimental group 1 is the method for the present invention, utilizes the plant growth lamp of customization to carry out Pachyrhizua angulatus tissue cultured seedling cultivation, cultivates working time selection
During February, comprise the following steps:
(1) building booth: build the booth of steel frame construction, the side of booth is provided with shutter door, is used for rolling or putting down, in order to adjust
Joint temperature of shed so that temperature of shed is stable between 20-25 DEG C；Booth shoulder height 1.4-1.7 rice, facilitates personnel to be managed.
(2) customized plant growth lamp: determine the quantity of plant growth lamp according to the area of set up booth.By calculating, customized is every
The effective range of unit plant growth lamp illumination is 3.5 square metres, and customized plant growth lamp uses single lamp holder power to be 3W, adopt
LED by 220v alternating current power supply etc., as luminous source, are 55 in conjunction with the quantity of every unit design lamp holder described in Fig. 1, wherein
Red 43, lamp holder, blue 12, lamp holder；Described red lamp holder quantity is 7:2 with blue-ray light lamp holder quantitative proportion, such as Fig. 1
The shown ratio installation connecting blue lamp holders (2) according to 3 red lamp holders (1)；
(3) seedling medium selects: select clean loess, yellow sand, fertilizer as seedling medium；Described fertilizer is sheep stool, chicken
Excrement, peanut residue, tea bran；Described substrate mix homogeneously is stand-by；Loess in seedling medium, yellow sand, fertilizer ratio are 1:1:
0.2；Sheep stool in described fertilizer, chicken manure, peanut residue, tea bran mix fermentation according to the ratio of 1:1:0.5:0.2.Because
In substrate, the content of organic matter is low, and temperature of shed is relatively low, can be prevented effectively from the situation of fungal infection.
(4) Pachyrhizua angulatus tissue cultured seedling is selected: Pachyrhizua angulatus tissue cultured seedling is placed in the water of 20 DEG C cleaning, culture medium is cleaned up；Cleaning is done
Clean tissue cultured seedling hymexazol is soaked, and the time that described hymexazol is soaked is 5min；Tissue cultured seedling after soaking is placed in shady and cool ventilation
Place carry out seedling exercising, it is to avoid blowing or the sun directly shine.
(5) nursery: select basal diameter, the round plastic nutrient cup of a height of 8cm*9cm to carry out nursery, by equal for mixing obtained above
Even seedling medium loads in each nutrient cup, described seedling medium pave after from nutrient cup rim of a cup 1-2cm.Described nutrient cup with
Centered by the wick of each described plant growth lamp, to be limited in the range of surrounding 3.5 meters, near the Pachyrhizua angulatus tissue cultured seedling growth of wick
Speed than away from the Pachyrhizua angulatus tissue cultured seedling of wick group high or sturdy luxuriant time, will be far from the powder that plant lamp growing way is short or slice is withered
Pueraria lobota tissue cultured seedling and the high or sturdy luxuriant Pachyrhizua angulatus tissue cultured seedling reversing of position near plant growth lamp growing way, reach to grow consistent mesh
's.
(6) nursery prophase management: after tissue cultured seedling is implanted nutrient cup 14-16 days is nursery early stage；Need the every day of each trickle sooner or later once,
Make humidity 18-30 DEG C in canopy, control humidity and be once watered the carbendazim of 300 times of liquid of dilution in 70%-90%, spray in every 7 days, and
Illumination duration is determined: in rainy weather, the illumination in 24 hours of plant growth lamp whole day according to rain or shine weather；At fine day, every day turns on light
Time controlled at 16-17 hour.
(7) nursery middle term management: nursery is after nursery early stage 10-12 days mid-terms, when tissue cultured seedling substantially enters the growth vigorous stage,
Leaf growth is obvious, and root system stretches.8 o'clock every mornings needed trickle once by 9 o'clock, opened booth simultaneously, ventilation, also
Play seedling exercising effect.Plant growth lamp lighting time: about 14 hours every day of fine day, 16-18 hour overcast and rainy every day.
(8) nursery final-period management: whole day opens booth, makes ventilation in booth, on the one hand plays seedling exercising effect, on the other hand fall
Low humidity prevents the fungal diseases such as powdery mildew, downy mildew, mycete from occurring；Run into Pachyrhizua angulatus tissue cultured seedling seedling-raising cup soil dry just to turn white
Needing to water at once, occurring without Pachyrhizua angulatus tissue cultured seedling seedling-raising cup soil, dry 1-2 days trickles are once when turning white；When plant lamp is turned on light every day
Between control at 10-12 hour.Plant lamp lighting time every day controlled at 10-12 hour.
Experimental result: after the Pachyrhizua angulatus Seedling entrance vigorous period that the method is cultivated, as a example by 1000 strains, the speed of growth is very fast,
Survival rate is more than 98%, and wherein, strong sprout, rate was 92%, and fungal infection rate is 0.5%.
Experimental group 2
Utilizing common electric filament lamp to carry out Pachyrhizua angulatus tissue cultured seedling cultivation, the cultivation working time selects during February, comprises the following steps:
Management mode that experimental group 2 is used, experimental procedure, management method, geographical environment are replaced except plant growth lamp electric filament lamp
In addition, remaining all of management mode, experimental procedure, management method, geographical environment are identical with experimental group 1.
Experimental result: after the Pachyrhizua angulatus Seedling entrance trophophase that the method is cultivated, the speed of growth is relatively slow, and survival rate is 35%, wherein,
Strong sprout, rate was 12%, and fungal infection rate is 0.52%.
Experimental group 3
The Pachyrhizua angulatus tissue cultured seedling of group without light is cultivated, and the cultivation working time selected during February, comprised the following steps:
Management mode that experimental group 3 is used, experimental procedure, management method, geographical environment in addition to without plant growth lamp, its
Remaining all of management mode, experimental procedure, management method, geographical environment are identical with experimental group 1.
Experimental result: after the Pachyrhizua angulatus Seedling entrance trophophase that the method is cultivated, growth survival is undesirable, and survival rate is 15%,
Wherein, strong sprout, rate was 0%, and fungal infection rate is 0.5%.
Above-mentioned experimental group 1, experimental group 2, experimental group 3 comparing result such as following table:
Table one: different groups comparison nursery situation (not heating, respectively select 1000 young plants):
From table, data can draw, after the Pachyrhizua angulatus Seedling entrance trophophase that the inventive method is cultivated, the speed of growth is relatively slow, survival rate
Being 35%, wherein, strong sprout, rate was 12%, and fungal infection rate is 0.52%.And the Pachyrhizua angulatus Seedling cultivated through comparative example one method enters
After trophophase, the speed of growth is relatively slow, and survival rate is 35%, and wherein, strong sprout, rate was 12%, and fungal infection rate is 0.52%；Warp
After crossing the Pachyrhizua angulatus Seedling entrance trophophase that comparative example two method is cultivated, growth survival is undesirable, and survival rate is 15%, wherein,
Strong sprout, rate was 0%, and fungal infection rate is 0.5%.
In sum, utilizing customized plant growth lamp plus perfect management, Pachyrhizua angulatus tissue cultured seedling can be carried out effectively during cold snap
Cultivation, carry out sufficiently preparation for transplanting land for growing field crops.
It should be pointed out that, that being above embodiment is only the example that the present invention is representative, technical scheme is not limited in above-mentioned
Embodiment, it is also possible to have many deformation.
Claims (7)
1. the method utilizing plant growth lamp to cultivate Pachyrhizua angulatus tissue cultured seedling, comprises the steps:
(1) building booth: build the booth of steel frame construction, the side of booth is provided with shutter door, booth shoulder height 1.4-1.7 rice；
(2) customized plant growth lamp: the effective range of customized every unit plant growth lamp illumination is 3.5 square metres, customized plants
Thing growth lamp uses single lamp holder power to be 3W, uses the LED etc. of 220v alternating current power supply as luminous source, and described every unit sets
The quantity of meter lamp holder is 55, red 43, lamp holder in described lamp holder, blue 12, lamp holder, and described red lamp holder quantity is with blue
Light lamp holder quantitative proportion is 7:2, and the ratio connecting a blue lamp holder (2) according to 3 red lamp holders (1) is installed, described
Red lamp holder is used for connecting red light, and described blue lamp holder is used for connecting blue lamp；
(3) seedling medium selects: select clean loess, yellow sand, fertilizer as seedling medium；Described fertilizer be sheep stool,
Chicken manure, peanut residue, tea bran；
(4) select Pachyrhizua angulatus tissue cultured seedling: Pachyrhizua angulatus tissue cultured seedling is placed in the water of 19-21 DEG C cleaning, the tissue cultured seedling cleaned up is used
Hymexazol is soaked；Tissue cultured seedling after soaking is placed in the place of shady and cool ventilation and carries out seedling exercising；
(5) nursery: selection basal diameter is 8cm, the round plastic nutrient cup of a height of 9cm carries out nursery, by described step (3)
The seedling medium of the mix homogeneously obtained loads in each nutrient cup, described seedling medium pave after from nutrient cup rim of a cup 1-2cm；
The tissue cultured seedling of select in described step (4) is implanted in described each nutrient cup installing substrate respectively；
(6) nursery prophase management: after tissue cultured seedling is implanted nutrient cup 14-16 days is nursery early stage；Need the every day of each trickle sooner or later
Once, make humidity 18-30 DEG C in canopy, control humidity and be watered 300 times of liquid of dilution in 70%-90%, concentration of spray in every 7 days
Carbendazim；
(7) nursery middle term management: nursery is after nursery early stage 10-12 days mid-terms, and 8 o'clock every mornings needed trickle one by 9 o'clock
Secondary, open booth simultaneously, ventilation, plant growth lamp lighting time: about 14 hours every day of fine day, overcast and rainy every day
16-18 hour；
(8) nursery final-period management: whole day opens booth, makes ventilation in booth, and 1-2 days trickles are once；Plant lamp every day
Lighting time controlled at 10-12 hour；
(9) transplant: the Pachyrhizua angulatus seedling obtained through step (8), transplant immediately when running into applicable field-transplanting environment.
A kind of method utilizing plant growth lamp to cultivate Pachyrhizua angulatus tissue cultured seedling, it is characterised in that described
Pull-up shutter door when the temperature of shed of step (1) is higher than 30 DEG C；Shutter door is put down when temperature of shed is less than 15 DEG C.
A kind of method utilizing plant growth lamp to cultivate Pachyrhizua angulatus tissue cultured seedling, it is characterised in that described
Loess in the seedling medium of step (3), yellow sand, fertilizer ratio are 1:1:0.2；Sheep stool in described fertilizer, chicken
Excrement, peanut residue, tea bran mix fermentation according to the ratio of 1:1:0.5:0.2.
A kind of method utilizing plant growth lamp to cultivate Pachyrhizua angulatus tissue cultured seedling, it is characterised in that described
The time that the tissue cultured seedling hymexazol that step (4) cleans up is soaked is 4-5min.
A kind of method utilizing plant growth lamp to cultivate Pachyrhizua angulatus tissue cultured seedling, it is characterised in that described
Nutrient cup in step (5) is centered by the wick of each described plant growth lamp, to be limited in the range of surrounding 3.5 meters, and point row
It is marked each nutrient cup by numeral；Higher than the Pachyrhizua angulatus tissue cultured seedling away from wick group near the Pachyrhizua angulatus tissue cultured seedling speed of growth of wick
Or sturdy luxuriant time, will be far from the Pachyrhizua angulatus tissue cultured seedling that plant lamp growing way is short or slice is withered high with near plant growth lamp growing way
Or sturdy luxuriant Pachyrhizua angulatus tissue cultured seedling reversing of position.
A kind of method utilizing plant growth lamp to cultivate Pachyrhizua angulatus tissue cultured seedling, it is characterised in that described step
Suddenly the nursery early stage of (6), in rainy weather, the illumination in 24 hours of plant growth lamp whole day；In fine day, lighting time control every day
Make at 16-17 hour.
A kind of method utilizing plant growth lamp to cultivate Pachyrhizua angulatus tissue cultured seedling, it is characterised in that Jing Guosuo
State the transplanting in step (9), run into weather temperature higher than 35 DEG C or weather temperature less than 10 DEG C or the most overcast and rainy unfavorable
Condition, land for growing field crops is not suitable for transplanting, plant growth lamp lighting time described above is brought up to 24 hours；Deng to land for growing field crops weather ring
Border is stable when 15 DEG C-25 DEG C, transplants immediately.
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| CN107980526A (en) * | 2017-11-29 | 2018-05-04 | 广西藤县绿葛葛业有限公司 | A kind of method for culturing seedlings of Pachyrhizua angulatus seedling |
| CN108934583A (en) * | 2018-06-25 | 2018-12-07 | 合肥海银生态农业有限公司 | A kind of energy-saving cultivating seedlings case in gardens |
| CN109451907A (en) * | 2018-11-20 | 2019-03-12 | 华能伊敏煤电有限责任公司 | Reproduce organic indigenous method |
| CN109618851A (en) * | 2018-12-20 | 2019-04-16 | 广西壮族自治区农业科学院生物技术研究所 | A kind of method of pueraria lobata nursery |
| CN110603998A (en) * | 2019-10-14 | 2019-12-24 | 四川蜀秀灯具有限公司 | Cultivation method for promoting anti-season coming-on-market of oranges, apples, grapes and kiwi fruits |
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| CN110603998A (en) * | 2019-10-14 | 2019-12-24 | 四川蜀秀灯具有限公司 | Cultivation method for promoting anti-season coming-on-market of oranges, apples, grapes and kiwi fruits |
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