CN105900741B - A method of Pachyrhizua angulatus tissue-cultured seedling is cultivated using plant growth lamp - Google Patents

A method of Pachyrhizua angulatus tissue-cultured seedling is cultivated using plant growth lamp Download PDF

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CN105900741B
CN105900741B CN201610292172.XA CN201610292172A CN105900741B CN 105900741 B CN105900741 B CN 105900741B CN 201610292172 A CN201610292172 A CN 201610292172A CN 105900741 B CN105900741 B CN 105900741B
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tissue
seedling
lamp
plant growth
pachyrhizua angulatus
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CN105900741A (en
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王艳
张尚文
欧昆鹏
王学礼
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Guangxi Zhuang Nationality Autonomous Region Academy of Agricultural Sciences
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Biotechnology Research Institute Guangxi Academy Of Agricultural Sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G9/00Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
    • A01G9/14Greenhouses
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/04Electric or magnetic or acoustic treatment of plants for promoting growth
    • A01G7/045Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/25Greenhouse technology, e.g. cooling systems therefor
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/14Measures for saving energy, e.g. in green houses

Abstract

The present invention provides a kind of method for cultivating Pachyrhizua angulatus tissue-cultured seedling using plant growth lamp, comprising the following steps: builds greenhouse, determination and customization plant growth lamp, seedling medium selection, selects Pachyrhizua angulatus tissue-cultured seedling, nursery, seedling management.The greenhouse is common steel frame construction, and the side of greenhouse is equipped with shutter door, for rolling or putting down, to adjust temperature of shed;The plant growth lamp uses lamp cap power for 3W, using the LED of 220v AC power source as light emitting source, the matrix selects clean loess, yellow sand and organic fertilizer and ratio is 1:1:0.2, and the organic fertilizer group becomes sheep dung, chicken manure, peanut press pulp, tea bran and mixes fermentation according to the ratio of 1:1:0.5:0.2.Pachyrhizua angulatus tissue-cultured seedling high survival rate is cultivated using this method, the seedling raise period that in the prior art 2, March just can be carried out can be advanced to and start to carry out nursery December previous year, the Pueraria lobota potato that is staggered concentrates listing peak, increases economic efficiency, and saves time cost.

Description

A method of Pachyrhizua angulatus tissue-cultured seedling is cultivated using plant growth lamp
Technical field
Present invention industry crop plants tissue cultivating technical field concerning farmers, it is especially a kind of to cultivate Pachyrhizua angulatus using plant growth lamp The method of tissue-cultured seedling.
Background technique
Pachyrhizua angulatus, the alias of pueraria lobata, also known as dry Pueraria lobota, Gan Ge are the root tuber of the perennial liana Pueraria lobota of pulse family, because forming sediment containing volume Powder (content is 19~20% in fresh pueraria lobata), it is therefore named.Elegant jessamine is yield height, using wide kind;And Pachyrhizua angulatus is with artificial cultivation Based on.Eating Pachyrhizua angulatus has many benefits to our body, and not only there is Pachyrhizua angulatus rising Yang expelling pathogenic factors from muscles and skin, promoting eruption antidiarrheal, relieving restlessness to quench the thirst Effect, and there is clearing heat and detoxicating effect, while Pachyrhizua angulatus also has the function of alleviating headache, day usually eats Pachyrhizua angulatus can also let us The quiet of mood is kept, a healthy dormancy of liking to sleep is possessed;It is used as medicine and treats hypertension, angina pectoris, gastric ulcer, gastrospasm, gastritis The diseases such as property pain;It is both parts of generic medicinal plants and diet good merchantable brand.Pachyrhizua angulatus can be processed into kudzuvine root starch, Pueraria lobota piece is refined into graininess speed Also extractable Puerarin of molten kudzuvine root starch, kudzu root tea etc..
So the cultivation of Pachyrhizua angulatus tissue-cultured seedling is worth with important research.Pachyrhizua angulatus growth is comprehensive by humidity, temperature, illumination etc. The influence of factor, at present in production, Pachyrhizua angulatus tissue-cultured seedling seedling-raising technique mainly uses simple plastic greenhouse seedling raising manners, and nursery work is general Carry out in 2, March, northern area is later.Simple plastic greenhouse seedling-raising technique mainly using scaffolding, improves temperature of shed, and use is light Seedling medium or sandy soil are cultivation way, cultivate tissue-cultured seedling.Cooperate certain watering, fertilising, cultivates suitable field production Seedling.For the low problem of Pachyrhizua angulatus tissue culture shoot survival percent, also someone uses fluorescent lamp light filling or fluorescent lamp light filling, effect It is substantially not visible.The growth period of Pachyrhizua angulatus is 10~December, is limited in production by seedling-growing time at present, generally in mid-April Just start transplanting plantation later, 1, the 2 months harvest to second year concentrates listing, and it is demonstrated experimentally that in Pachyrhizua angulatus main product, 3,4, The weather conditions in May are Pachyrhizua angulatus nutrient growth vigorous periods, and therefore, seedling raise period was mentioned to 2 months, can be big for Pachyrhizua angulatus Field high yield is taken a firm foundation, and is saved time cost, is obtained Pachyrhizua angulatus finished product ahead of time, be staggered and list peak;But before 2 months, Since weather is sombre, temperature is low, the illuminance reasons such as not enough, causes Pachyrhizua angulatus tissue-cultured seedling cultivate effect poor, quality is low, main to show In low seedling survival rate, the seedling that survives occurs that stalk is thin, excessive growth because being influenced by the inadequate factor of temperature and effective illuminance, Transplant the problems such as resistance is low, also occur in production to improve the method that the survival rate of tissue-cultured seedling improves temperature of shed, as a result because Temperature and humidity is higher, causes a large amount of tissue-cultured seedling mycotic infection phenomenon, by drugs such as sprinkling carbendazim, is often also difficult to inhibit. Time and the quality for directly affecting field-transplanting, improve production cost.
The present invention is in order to solve the disadvantage that provide a kind of new Pachyrhizua angulatus tissue culture seeding cultivating method in the prior art, effectively Solution to carry out the training of Pachyrhizua angulatus tissue-cultured seedling low seedling survival rate, effective illuminance before annual 2 month in the past inadequate, there is stalk Carefully, the problem of resistance is low equal is transplanted in excessive growth.
Summary of the invention
For the variety of problems faced described in background technique, plant growth lamp is utilized the purpose of the present invention is to provide a kind of The method for cultivating Pachyrhizua angulatus tissue-cultured seedling.In order to achieve the above objectives, the technical scheme adopted by the invention is that:
A kind of method for cultivating Pachyrhizua angulatus tissue-cultured seedling using plant growth lamp of the present invention, includes the following steps:
It builds greenhouse: building the greenhouse of steel frame construction, the side of the greenhouse is equipped with shutter door, for rolling or putting Under, to adjust temperature of shed, so that temperature of shed is stablized between 20-25 DEG C;1.4-1.7 meters of greenhouse shoulder height, facilitate personnel It is managed.
Further, it is determined that the quantity of plant growth lamp and customizing plant growth lamp: the area according to established greenhouse is true Determine the quantity of plant growth lamp.By calculating, the effective range of customized each plant growth lamp is 3.5 squares.Customized plant Object growth lamp: the effective range of customized every unit plant growth lamp illumination is 3.5 square metres, and customized plant growth lamp is using single A lamp cap power is 3W, is used as light emitting source using LED of 220v AC power source etc., and the quantity of every unit design lamp cap is 55 A, red 43, lamp cap in the lamp cap, 12, blue lamp cap connects a blue lamp cap installation, institute according to 3 red lamp caps Red lamp cap is stated for connecting red light, the blue lamp cap is for connecting blue lamp;Plant growth lamp shape uses box structure, interior Set fan cooling piece.
Further, seedling medium selects: selecting clean loess, yellow sand and organic fertilizer as seedling medium;It is described to have Machine fertilizer is sheep dung, chicken manure, peanut press pulp, tea bran;The matrix is uniformly mixed stand-by;Loess, yellow sand in seedling medium and organic Fertile ratio is 1:1:0.2;Sheep dung, chicken manure, peanut press pulp, tea bran in the organic fertilizer are mixed according to the ratio of 1:1:0.5:0.2 Fermentation.The case where because content of organic matter is low in matrix, temperature of shed is lower, can effectively avoid mycotic infection.
Further, it selects Pachyrhizua angulatus tissue-cultured seedling: Pachyrhizua angulatus tissue-cultured seedling being placed in 19-21 DEG C of water and is cleaned, culture medium is clear Wash clean;The tissue-cultured seedling cleaned up is impregnated with hymexazol, the time that the hymexazol is impregnated is 4-5min;After immersion The place that tissue-cultured seedling is placed in shady and cool ventilation carries out hardening, and blowing or the sun is avoided directly to shine.
Further, selecting basal diameter is 8cm, the round plastic nutrition cup of a height of 9cm carries out nursery, is obtained above-mentioned Uniformly mixed seedling medium be fitted into each nutrition cup, the seedling medium pave after from nutrition cup rim of a cup 1-2cm.
Further, the Pachyrhizua angulatus tissue-cultured seedling of above-mentioned select is implanted into respectively and described is each installed in the nutrition cup of matrix. Array type square arrangement is pressed in the arrangement of nutrition cup, and nutrition cup is centered on the wick of each plant growth lamp, with surrounding 3.5 It is limited in rice range, divides row that each nutrition cup is marked with number;Train the nutrition cup of seedling and the nutrition far from wick tissue-cultured seedling Cup carries out location swap.Pachyrhizua angulatus tissue culture height of seedling of the Pachyrhizua angulatus tissue-cultured seedling speed of growth than separate wick or sturdy cyclopentadienyl close to wick Flower will be far from plant lamp growing way is short or slice is withered Pachyrhizua angulatus tissue-cultured seedling and close plant growth lamp growing way height or sturdy Luxuriant Pachyrhizua angulatus tissue-cultured seedling reversing of position reaches the consistent purpose of growth.Be conducive in different times according to different growing states Exchanged, by low beam center tissue-cultured seedling growth can significantly better than periphery, therefore cup type cultivation can according to growing state, into Row is exchanged, and it is consistent to reach growth.
Further, nursery prophase management: being nursery early period by 14-16 days after tissue-cultured seedling implantation nutrition cup;It needs daily Sooner or later each water drenching is primary, makes 18-30 DEG C of humidity in canopy, and control humidity is to be watered in 70%-90%, spray in every 7 days concentration Dilute the carbendazim of 300 times of liquid, and determine illumination duration according to rain or shine weather: in rainy weather, plant growth lamp whole day 24 is small Shi Zhaoming;In fine day, daily lighting time control is in 16-17 hour.
Further, nursery middle term management: nursery mid-term is 10-12 days after nursery early period, when tissue-cultured seedling obviously enters life In the long vigorous stage, leaf growth is obvious, root system stretching, extension.8 points to 9 points of every morning needs water drenching primary, opens simultaneously greenhouse, leads to Wind ventilation also functions to hardening effect.Plant growth lamp lighting time: or so fine day daily 14 hours, rainy days daily 16-18 A hour.
Further, nursery final-period management: whole day opens greenhouse, makes ventilation in greenhouse, on the one hand plays hardening work With on the other hand reducing humidity prevents the fungal diseases such as powdery mildew, downy mildew, mould from occurring;Encounter Pachyrhizua angulatus tissue-cultured seedling nursery The native hair shaft of cup, which whitens, just to be needed to water at once, and it is primary not occur water drenching in 1-2 days when Pachyrhizua angulatus tissue-cultured seedling seedling-raising cup soil hair shaft whitens;It plants The daily lighting time control of object lamp is in 10-12 hour.
Further, transplanting in due course, Pachyrhizua angulatus seedling enters after vigorous period, after the speed of growth very grows up to seedling fastly, Weather temperature is encountered higher than 35 DEG C or weather temperature is lower than 10 DEG C or continuous overcast and rainy unfavorable conditions, at this point, crop field is uncomfortable Transplanting is closed, plant growth lamp lighting time described above is increased to 24 hours, full sun illumination, temporarily inhibits powder to reach The purpose of Ge Miao excessive growth;Stablize Deng to crop field climatic environment at 20 DEG C -30 DEG C, at this point, crop field is suitble to transplant, can immediately into Row transplanting.
The invention has the following advantages:
1, can overcome just can be carried out after having in the prior art to March Pachyrhizua angulatus tissue-cultured seedling cultivate work be advanced to One annual December cultivated, and overcame that 2 months December to coming year weather is sombre, temperature is low, illuminance not enough makes Pachyrhizua angulatus culture seedling The low technological difficulties of survival rate.
2, cultivate and nutrition cup is marked respectively centered on plant growth lamp wick using nutrition cup, favorably It is exchanged in different times according to different growing states, the tissue-cultured seedling growth by low beam center can be significantly better than periphery, because This cup type cultivation can be exchanged according to growing state, and it is consistent to reach growth.
3, in the case where not needing to promote temperature of shed, increase effective illumination, the survival rate and strong sprout rate of tissue-cultured seedling Comparison former years are significantly improved.It does not heat up, greatly suppresses the infection of mould.
4, nursery after March cultivates the time too late, and Pachyrhizua angulatus is short in big field growth time, and normal growth is not achieved Time cannot tie potato for the year or yield is very low.
Detailed description of the invention
Fig. 1 is plant growth lamp unit layout viewing
Description of symbols:
1 circle represents red lamp cap, and 2 triangles represent blue lamp cap
Specific embodiment
Make below with reference to the different embodiments method for cultivating Pachyrhizua angulatus tissue-cultured seedling using plant growth lamp a kind of to the present invention It further illustrates.
Embodiment one
As shown in connection with fig. 1, Fig. 1 is the layout viewing of every unit plant growth lamp, carries out powder using the plant growth lamp of customization Pueraria lobota tissue-cultured seedling is cultivated, and cultivates working time selection during 2 months next years of December-, comprising the following steps:
(1) it builds greenhouse: building the greenhouse of steel frame construction, the side of greenhouse is equipped with shutter door, for rolling or putting down, To adjust temperature of shed, so that temperature of shed is stablized between 20-25 DEG C;1.4-1.7 meters of greenhouse shoulder height, personnel is facilitated to carry out Management.
(2) quantity of plant growth lamp customized plant growth lamp: is determined according to the area of established greenhouse.By calculating, The effective range of customized every unit plant growth lamp illumination is 3.5 square metres, and customized plant growth lamp uses single lamp cap function Rate is 3W, is used as light emitting source using LED of 220v AC power source etc., and the quantity of every unit design lamp cap is 55 in conjunction with described in Fig. 1 It is a, wherein red 43, lamp cap, 12, blue lamp cap;As shown in Figure 1 according to 3 red lamp caps (1) around one blue of connection Lamp cap (2) installation;The red lamp cap is for connecting red light, and the blue lamp cap is for connecting blue lamp;Every unit plant growth Lamp shape uses box structure, built-in fans cooling fin.
(3) seedling medium selects: selecting clean loess, yellow sand and organic fertilizer as seedling medium;The organic fertilizer is Sheep dung, chicken manure, peanut press pulp, tea bran;The matrix is uniformly mixed stand-by;Loess, yellow sand and organic fertilizer ratio in seedling medium For 1:1:0.2;Sheep dung, chicken manure, peanut press pulp, tea bran in the organic fertilizer mix fermentation according to the ratio of 1:1:0.5:0.2.Cause The case where low for the content of organic matter in matrix, temperature of shed is lower, can effectively avoid mycotic infection.
(4) it selects Pachyrhizua angulatus tissue-cultured seedling: Pachyrhizua angulatus tissue-cultured seedling being placed in 19-21 DEG C of water and is cleaned, culture medium is cleaned up; The tissue-cultured seedling cleaned up is impregnated with hymexazol, the time that the hymexazol is impregnated is 4-5min;By the tissue-cultured seedling after immersion The place for being placed in shady and cool ventilation carries out hardening, and blowing or the sun is avoided directly to shine.
(5) nursery: selecting the typical circular plastic nutritional cup of basal diameter, a height of 8cm*9cm to carry out nursery, obtains above-mentioned To uniformly mixed seedling medium be fitted into each nutrition cup, the seedling medium pave after from nutrition cup rim of a cup 1-2cm.It will The Pachyrhizua angulatus tissue-cultured seedling of above-mentioned select is implanted into respectively described each to install in the nutrition cup of matrix.Array type is pressed in the arrangement of nutrition cup Square arrangement, the nutrition cup is centered on the wick of each plant growth lamp, to be limited within the scope of 3.5 meters of surrounding, point Each nutrition cup is marked with number in row.The Pachyrhizua angulatus tissue-cultured seedling speed of growth close to wick trains seedling than the Pachyrhizua angulatus of separate wick group Will be far from that plant lamp growing way is short when high perhaps sturdy luxuriant or Pachyrhizua angulatus tissue-cultured seedling that slice is withered and close plant growth lamp it is long Gesture height or sturdy luxuriant Pachyrhizua angulatus tissue-cultured seedling reversing of position reach the consistent purpose of growth.
(6) nursery prophase management: being nursery early period by 14-16 days after tissue-cultured seedling implantation nutrition cup;It needs sooner or later each daily Water drenching is primary, makes 18-30 DEG C of humidity in canopy, and control humidity is to be watered dilution 300 in 70%-90%, spray in every 7 days concentration The carbendazim of times liquid, and illumination duration is determined according to rain or shine weather: in rainy weather, illuminate within plant growth lamp whole day 24 hours; In fine day, daily lighting time control is in 16-17 hour.
(7) nursery middle term management: nursery mid-term is 10-12 days after nursery early period, when tissue-cultured seedling is obviously vigorous into growing In the stage, leaf growth is obvious, root system stretching, extension.8 points to 9 points of every morning needs water drenching primary, opens simultaneously greenhouse, and ventilation is changed Gas also functions to hardening effect.Plant growth lamp lighting time: or so fine day daily 14 hours, rainy days are daily 16-18 small When.
(8) nursery final-period management: whole day opens greenhouse, makes ventilation in greenhouse, on the one hand plays the role of hardening, another Aspect, which reduces humidity, prevents the fungal diseases such as powdery mildew, downy mildew, mould from occurring;It is dry to encounter Pachyrhizua angulatus tissue-cultured seedling seedling-raising cup soil Whiten and just need to water at once, it is primary not occur water drenching in 1-2 days when Pachyrhizua angulatus tissue-cultured seedling seedling-raising cup soil hair shaft whitens;Plant lamp is daily Lighting time was controlled in 10-12 hour.The daily lighting time control of plant lamp is in 10-12 hour.
(9) transplant: the Pachyrhizua angulatus seedling obtained by step (8) is moved immediately when encountering suitable field-transplanting environment It plants;Weather temperature is encountered higher than 35 DEG C or weather temperature is lower than 10 DEG C or continuous overcast and rainy unfavorable conditions, crop field are uncomfortable Transplanting is closed, plant growth lamp lighting time described above is increased to 24 hours;Deng to crop field climatic environment stablize 20 DEG C- At 30 DEG C, transplanted immediately.
In order to further illustrate the present invention the advantages of, further illustrates result below by three groups of comparative experimentss, every group Comparative experiments is by taking 1000 plants of seedling as an example:
Experimental group one
Experimental group 1 is method of the invention, carries out the cultivation of Pachyrhizua angulatus tissue-cultured seedling using the plant growth lamp of customization, cultivates work Selection of time is during 2 months, comprising the following steps:
(1) it builds greenhouse: building the greenhouse of steel frame construction, the side of greenhouse is equipped with shutter door, for rolling or putting down, To adjust temperature of shed, so that temperature of shed is stablized between 20-25 DEG C;1.4-1.7 meters of greenhouse shoulder height, personnel is facilitated to carry out Management.
(2) quantity of plant growth lamp customized plant growth lamp: is determined according to the area of established greenhouse.By calculating, The effective range of customized every unit plant growth lamp illumination is 3.5 square metres, and customized plant growth lamp uses single lamp cap function Rate is 3W, is used as light emitting source using LED of 220v AC power source etc., and the quantity of every unit design lamp cap is 55 in conjunction with described in Fig. 1 It is a, wherein red 43, lamp cap, 12, blue lamp cap;As shown in Figure 1 according to 3 red lamp caps (1) around one blue of connection Lamp cap (2) installation;
(3) seedling medium selects: selecting clean loess, yellow sand and organic fertilizer as seedling medium;The organic fertilizer is Sheep dung, chicken manure, peanut press pulp, tea bran;The matrix is uniformly mixed stand-by;Loess, yellow sand and organic fertilizer ratio in seedling medium For 1:1:0.2;Sheep dung, chicken manure, peanut press pulp, tea bran in the organic fertilizer mix fermentation according to the ratio of 1:1:0.5:0.2.Cause The case where low for the content of organic matter in matrix, temperature of shed is lower, can effectively avoid mycotic infection.
(4) it selects Pachyrhizua angulatus tissue-cultured seedling: Pachyrhizua angulatus tissue-cultured seedling being placed in 20 DEG C of water and cleaned, culture medium is cleaned up;It will The tissue-cultured seedling cleaned up is impregnated with hymexazol, and the time that the hymexazol is impregnated is 5min;Tissue-cultured seedling after immersion is placed in The place of shady and cool ventilation carries out hardening, and blowing or the sun is avoided directly to shine.
(5) nursery: selecting the round plastic nutrition cup of basal diameter, a height of 8cm*9cm to carry out nursery, will be obtained above Uniformly mixed seedling medium is fitted into each nutrition cup, the seedling medium pave after from nutrition cup rim of a cup 1-2cm.The battalion Cup is supported centered on the wick of each plant growth lamp, to be limited within the scope of 3.5 meters of surrounding, close to the Pachyrhizua angulatus tissue culture of wick The seedling speed of growth will be far from that plant lamp growing way is short when perhaps sturdy more luxuriant than the Pachyrhizua angulatus tissue culture height of seedling of separate wick or slice is dry Withered Pachyrhizua angulatus tissue-cultured seedling and close plant growth lamp growing way height or sturdy luxuriant Pachyrhizua angulatus tissue-cultured seedling reversing of position, reach growth Consistent purpose.
(6) nursery prophase management: being nursery early period by 14-16 days after tissue-cultured seedling implantation nutrition cup;It needs sooner or later each daily Water drenching is primary, makes 18-30 DEG C of humidity in canopy, and control humidity is to be watered dilution 300 in 70%-90%, spray in every 7 days concentration The carbendazim of times liquid, and illumination duration is determined according to rain or shine weather: in rainy weather, illuminate within plant growth lamp whole day 24 hours; In fine day, daily lighting time control is in 16-17 hour.
(7) nursery middle term management: nursery mid-term is 10-12 days after nursery early period, when tissue-cultured seedling is obviously vigorous into growing In the stage, leaf growth is obvious, root system stretching, extension.8 points to 9 points of every morning needs water drenching primary, opens simultaneously greenhouse, and ventilation is changed Gas also functions to hardening effect.Plant growth lamp lighting time: or so fine day daily 14 hours, rainy days are daily 16-18 small When.
(8) nursery final-period management: whole day opens greenhouse, makes ventilation in greenhouse, on the one hand plays the role of hardening, another Aspect, which reduces humidity, prevents the fungal diseases such as powdery mildew, downy mildew, mould from occurring;It is dry to encounter Pachyrhizua angulatus tissue-cultured seedling seedling-raising cup soil Whiten and just need to water at once, it is primary not occur water drenching in 1-2 days when Pachyrhizua angulatus tissue-cultured seedling seedling-raising cup soil hair shaft whitens;Plant lamp is daily Lighting time was controlled in 10-12 hour.The daily lighting time control of plant lamp is in 10-12 hour.
Experimental result: after the Pachyrhizua angulatus seedling that the method is cultivated enters vigorous period, for 1000 plants, growth speed Degree is very fast, and survival rate is 98% or more, wherein strong sprout rate is 92%, and mycotic infection rate is 0.5%.
Experimental group 2
The cultivation of Pachyrhizua angulatus tissue-cultured seedling is carried out using common incandescent lamp, cultivates working time selection during 2 months, including following Step:
Management mode used by experimental group 2, experimental procedure, management method, geographical environment are in addition to plant growth lamp is with white Other than vehement lamp replacement, remaining all management mode, experimental procedure, management method, geographical environment are identical as experimental group 1.
Experimental result: after the Pachyrhizua angulatus seedling that the method is cultivated enters growth period, the speed of growth is slower, and survival rate is 35%, wherein strong sprout rate is 12%, and mycotic infection rate is 0.52%.
Experimental group 3
No light group Pachyrhizua angulatus tissue-cultured seedling is cultivated, and cultivates working time selection during 2 months, comprising the following steps:
Management mode used by experimental group 3, experimental procedure, management method, geographical environment be not in addition to having to plant growth lamp In addition, remaining all management mode, experimental procedure, management method, geographical environment are identical as experimental group 1.
Experimental result: after the Pachyrhizua angulatus seedling that the method is cultivated enters growth period, growth survival is undesirable, survival rate It is 15%, wherein strong sprout rate is 0%, and mycotic infection rate is 0.5%.
Above-mentioned experimental group 1, experimental group 2,3 comparing result of experimental group are as follows:
Table one: different groups compare nursery situation (not heating, respectively select 1000 young plants):
Data can obtain from table, by the method for the present invention cultivate Pachyrhizua angulatus seedling enter growth period after, the speed of growth compared with Slowly, survival rate 35%, wherein strong sprout rate is 12%, and mycotic infection rate is 0.52%.And pass through what one method of comparative example was cultivated Pachyrhizua angulatus seedling entered after growth period, and the speed of growth is slower, survival rate 35%, wherein strong sprout rate is 12%, and mycotic infection rate is 0.52%;After the Pachyrhizua angulatus seedling that two method of comparative example is cultivated enters growth period, growth survival is undesirable, and survival rate is 15%, wherein strong sprout rate is 0%, and mycotic infection rate is 0.5%.
In conclusion adding perfect management using customized plant growth lamp, Pachyrhizua angulatus tissue-cultured seedling is during cold snap It can be carried out effective cultivation, carry out sufficient preparation for transplanting crop field.
It should be pointed out that being the embodiment only representative example of the present invention above, technical solution of the present invention is not It is only limitted to above-described embodiment, acceptable there are many deformations.

Claims (7)

1. a kind of method for cultivating Pachyrhizua angulatus tissue-cultured seedling using plant growth lamp, includes the following steps:
(1) it builds greenhouse: building the greenhouse of steel frame construction, the side of greenhouse is equipped with shutter door, and 1.4-1.7 meters of greenhouse shoulder height;
(2) customized plant growth lamp: the effective range of customized every unit plant growth lamp illumination is 3.5 square metres, customized plant Object growth lamp uses single lamp cap power for 3W, using the LED light of 220v AC power source as light emitting source, every unit design The quantity of lamp cap is 55, red 43, lamp cap in the lamp cap, 12, blue lamp cap, and the red lamp cap is red for connecting Lamp, the blue lamp cap is for connecting blue lamp;
(3) seedling medium selects: selecting clean loess, yellow sand and organic fertilizer as seedling medium;The organic fertilizer be sheep dung, Chicken manure, peanut press pulp, tea bran;
(4) it selects Pachyrhizua angulatus tissue-cultured seedling: Pachyrhizua angulatus tissue-cultured seedling being placed in 19-21 DEG C of water and is cleaned, the tissue-cultured seedling cleaned up is used Hymexazol is impregnated;The place that tissue-cultured seedling after immersion is placed in shady and cool ventilation is subjected to hardening;
(5) nursery: selection basal diameter is 8cm, the round plastic nutrition cup of a height of 9cm carries out nursery, and the step (3) are obtained To uniformly mixed seedling medium be fitted into each nutrition cup, the seedling medium pave after from nutrition cup rim of a cup 1-2cm;It will The tissue-cultured seedling of select is implanted into respectively in the step (4) each installs in the nutrition cup of matrix;
(6) nursery prophase management: being nursery early period by 14-16 days after tissue-cultured seedling implantation nutrition cup;Need each water drenching sooner or later daily Once, make 18-30 DEG C of humidity in canopy, control humidity is to be watered 300 times of liquid of dilution in 70%-90%, spray in every 7 days concentration Carbendazim;
(7) nursery middle term management: nursery mid-term is 10-12 days after nursery early period, and 8 points to 9 points of every morning needs water drenching primary, Open simultaneously greenhouse, ventilation, plant growth lamp lighting time: or so fine day daily 14 hours, rainy days daily 16-18 A hour;
(8) nursery final-period management: whole day opens greenhouse, makes ventilation in greenhouse, water drenching in 1-2 days is primary;Plant lamp is opened daily The lamp time controlled in 10-12 hour;
(9) transplant: the Pachyrhizua angulatus seedling obtained by step (8) is transplanted immediately when encountering suitable field-transplanting environment.
2. a kind of method for cultivating Pachyrhizua angulatus tissue-cultured seedling using plant growth lamp as described in claim 1, it is characterised in that described Pull-up shutter door when the temperature of shed of step (1) is higher than 30 DEG C;Temperature of shed puts down shutter door when being lower than 15 DEG C.
3. a kind of method for cultivating Pachyrhizua angulatus tissue-cultured seedling using plant growth lamp as described in claim 1, it is characterised in that described Loess, yellow sand and organic fertilizer ratio in the seedling medium of step (3) are 1:1:0.2;Sheep dung, chicken manure in the organic fertilizer, Peanut press pulp, tea bran mix fermentation according to the ratio of 1:1:0.5:0.2.
4. a kind of method for cultivating Pachyrhizua angulatus tissue-cultured seedling using plant growth lamp as described in claim 1, it is characterised in that described The tissue-cultured seedling that step (4) cleans up is 4-5min with the time that hymexazol is impregnated.
5. a kind of method for cultivating Pachyrhizua angulatus tissue-cultured seedling using plant growth lamp as described in claim 1, it is characterised in that described Nutrition cup in step (5) is centered on the wick of each plant growth lamp, to be limited within the scope of 3.5 meters of surrounding, point row Each nutrition cup is marked with number;Pachyrhizua angulatus tissue culture height of seedling of the Pachyrhizua angulatus tissue-cultured seedling speed of growth of close wick than separate wick Perhaps the Pachyrhizua angulatus tissue-cultured seedling and close plant growth lamp growing way that plant lamp growing way is short or slice is withered be will be far from when sturdy luxuriant High or sturdy luxuriant Pachyrhizua angulatus tissue-cultured seedling reversing of position.
6. a kind of method for cultivating Pachyrhizua angulatus tissue-cultured seedling using plant growth lamp as described in claim 1, it is characterised in that described The nursery of step (6) illuminates early period for plant growth lamp whole day 24 hours in rainy weather;In fine day, daily lighting time control In 16-17 hour.
7. a kind of method for cultivating Pachyrhizua angulatus tissue-cultured seedling using plant growth lamp as described in claim 1, it is characterised in that pass through Transplanting in the step (9), encounter weather temperature higher than 35 DEG C or weather temperature lower than 10 DEG C or it is continuous it is overcast and rainy not Sharp condition, crop field are not suitable for transplanting, the plant growth lamp lighting time are increased to 24 hours;Deng arrive crop field climatic environment Stablize at 15 DEG C -25 DEG C, is transplanted immediately.
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