CN105532395A - Method for using mixed light medium for subprostrate sophora twig cutting seedling raising, and application - Google Patents
Method for using mixed light medium for subprostrate sophora twig cutting seedling raising, and application Download PDFInfo
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- CN105532395A CN105532395A CN201510915102.0A CN201510915102A CN105532395A CN 105532395 A CN105532395 A CN 105532395A CN 201510915102 A CN201510915102 A CN 201510915102A CN 105532395 A CN105532395 A CN 105532395A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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Abstract
The invention discloses a method for using a mixed light medium for subprostrate sophora twig cutting seedling raising, and applications. The method comprises: step 1, preparing a cutting bed; step 2, acquiring and processing cuttings; step 3, cutting; step 4, and performing later-period management. The mixed light medium containing grass carbon, subsoil, and coco coir is used for annual twig cutting seedling raising of subprostrate sophora. The method is characterized by light medium, good permeability, large seedling rooting rate, and obvious lateral root development. Compared with sown seedlings, after planting, initial growth rate is obviously increased, growth increment is large, and adaptability is good. The method is characterized by simple and feasible and efficient and cheap, and is fast in breeding speed and high in reproduction coefficient, and characteristics of superior strains can be preferably maintained. The method can be applied for actual production of subprostrate sophora artificial cultivation, and has relatively high economical benefits and social benefits.
Description
Technical field
The present invention relates to subprostrate sophora vegetative propagation technique field, particularly a kind of methods and applications utilizing mixing Light media to carry out subprostrate sophora epicormic branch cutting.
Background technology
Subprostrate sophora is the dry root and rhizome of overgrowing property of pulse family stunted brushwood plant sophora tonkinensis Gapnep SophoratonkinensisGagnep., main product in Guangxi, Guizhou.Subprostrate sophora begins to be loaded in " Kaibao Bencao ", and property of medicine bitter cold is poisonous, attaches to the lung and stomach meridians.It is clearing heat and detoxicating, and detumescence relieve sore throat effect is obvious.In clinical research in modern age, be usually used in the illnesss such as treatment abscess of throat, jaundice with damp-heat pathogen and arrhythmia cordis.
Subprostrate sophora natural regeneration, based on seed dispersal, easily occurs in artificial cultivation process that seed sprouting is irregular, the obvious problem of descendants specialize, is difficult to keep maternal excellent economic characters.Cottage propagation has simple, that effect height is inexpensive, reproduction speed is fast, reproduction coefficient is high feature, can directly apply to actual production.Cottage propagation, as vegetative propagation means, has the advantage that can keep elite stand good characteristic, and this is for the maintenance important in inhibiting of the excellent economic characters required by economic forest cultivation.Cuttage seeding is compared with tree seedling, and after planting, primary growth speed obviously increases, and amount of growth is large, and strong adaptability, therefore cuttage seeding is more satisfactory reforestation material.
At present, the research of the cuttage breeding method of existing subprostrate sophora, such as patent CN201310395453.4 discloses a kind of subprostrate sophora cuttage and seedling culture method, belong to technical field of Chinese herbal medicine cultivation, comprise concrete steps such as adopting branch, beta pruning, plant hormone immersion treatment, the preparation of husky bed, cuttage, trickle, shelter from heat or light, obtain subprostrate sophora cuttage wood seedling; Patent CN201310241841.7 has invented a kind of cuttage expanding propagation method of wild subprostrate sophora, comprises the following steps: the cutting of (1) wild subprostrate sophora stem; (2) stem section is loaded nutritious bag; (3) heat insulating culture 19 ~ 21 days; (4) move into land for growing field crops, obtain subprostrate sophora cuttage seeding.But, the deficiency that weight is large, permeability is poor, nursery stock rooting rate unstable, the phase of taking root is long, root system is less-developed, growth of cuttage seedlings gesture is weak that single matrix of taking root is brought is there is in the research at present about subprostrate sophora cuttage, especially show as that main root is obvious, lateral root development is bad, and being easily subject to the restriction in season, after afforestation, growth perfonnance is not good enough; Simultaneously the weight of matrix to transplanting seedlings, lifting, transport bring very large obstacle, add man power and material, be unfavorable for large-scale production and the Application and Development of subprostrate sophora cottage propagation.
Summary of the invention
Another object of the present invention is to provide a kind of method utilizing mixing Light media to carry out subprostrate sophora epicormic branch cutting, it utilizes mixing Light media, the annual spray of employing subprostrate sophora, cuttage and seedling culture method is carried out in the process of use mixing root-growing agent, cultivation period is short, root system is good, survival rate is high, easy and simple to handle, to transplant seedlings, lifting, convenient transport.
Another object of the present invention is to provide a kind of application of method in subprostrate sophora artificial culture utilizing mixing Light media to carry out subprostrate sophora epicormic branch cutting.
The present invention also provides a kind of method utilizing mixing Light media to carry out subprostrate sophora epicormic branch cutting, comprises the steps:
Step 1, cutting bed prepares: make cutting bed, lay stone and/or rough sand rebasing, configuration mixing Light media also loads in nutritious bag for subsequent use, and the component of described mixing Light media and volume ratio proportioning are the peat composed of rotten mosses: red heart soil: coconut palm chaff=(2-3): 2:(1-2);
Step 2, cuttings acquisition and processing: gather first lignified annual green branch of elite stand as cuttage cuttings, after pruning, sterilization, adopts hormone mixed solution immersion treatment;
Step 3, cuttage: matrix is evenly watered before cuttage, make substrate water content reach 67 ~ 73%, through the cuttings of hormone mixed solution process be inserted into mixing Light media is housed nutritious bag in, water immediately 1 time permeable to make substrate water content reach more than 90%;
Step 4, final-period management: the later stage carries out sheltering from heat or light and moisturizing process, manages until go out garden in conventional liquid manure mode, obtains the subprostrate sophora nursery stock of high-quality.
Preferably, during step 1 cutting bed prepares, select leeward on the sunny side, the place of good water permeability builds outdoor cutting bed, the stone that cuttage bed one deck 3 ~ 5cm is thick and/or rough sand rebasing.
Preferably, in step 2 cuttings acquisition and processing, mixing water and the alcoholic solution of described is indole-3-butyric acid (IBA) and methyl α-naphthyl acetate (NAA).
Preferably, in step 3 cuttage, first 1 day of cuttage with potassium manganate solution to the disinfection of mixing Light media.
Preferably, in step 3 cuttage, cutting time is that annual March is to November.
Preferably, in step 2 cuttings acquisition and processing, the fringe bar of semi-lignified is cut into the cuttings of 10 ~ 15cm; In step 3, cuttings lower end 3 ~ 5cm is put into hormone mixed solution and soaks; The cuttage degree of depth is 3 ~ 5cm.
Preferably, in step 4 final-period management: build shading shed and add a cover shading screen and carry out process of sheltering from heat or light, cutting bed plastic covering film carries out moisturizing process.
Preferably, step 4 final-period management comprises disease control, sprays carbendazim carry out disinfection after cuttage every 7 ~ 15 days.
More specifically, the method utilizing mixing Light media to carry out subprostrate sophora epicormic branch cutting comprises:
Step 1, cutting bed prepare: comprise
(1) cutting bed: select leeward on the sunny side, the place of good water permeability builds outdoor cutting bed, the length 6 ~ 10m of cutting bed, wide 1 ~ 1.2m, the rough sand that cuttage bed one deck 3 ~ 5cm is thick is rebasing;
(2) mix the preparation of Light media: for subsequent use with the peat composed of rotten mosses, red heart soil and the mixing of coconut palm chaff, as the mixing Light media of cuttage, Light media will be mixed and load in the nutritious bag of 8 × 12, and closely be placed in cutting bed; First 1 day of cuttage is that the potassium permanganate of 0.1% is to the disinfection of mixing Light media by concentration.Wherein, each volume components mixed in Light media matches well than being the peat composed of rotten mosses: red heart soil: coconut palm chaff=3:2:1; The peat composed of rotten mosses can be the forest peat composed of rotten mosses that Northeast Area of China gathers; Coconut palm chaff can be the coconut palm chaff fully become thoroughly decomposed according to a conventional method.
Step 2, cuttings acquisition and processing: comprise
(1) cuttings collection: the whole year all can arrange cuttage in South China, best cutting time be annual March to November, gather first lignified annual green branch of elite stand as cuttage cuttings; In the present embodiment, cuttage cuttings can be robust growth, annual green branch without damage by disease and insect, semi-lignified that elite stand gathers, is cut into 10 ~ 15cm as cuttage cuttings;
(2) prune cuttings: the cuttings fringe bar of semi-lignified being cut into 10 ~ 15cm, remove the blade of above about the 8cm of base portion, lower end angle, bud is close in upper end, flat mouth, ties with rubber band or thin cotton cord;
(3) disinfect: cuttings is neatly placed in container, soak with 0.5% carbendazim, sterilization 15 ~ 20min, taking-up clear water rinses stand-by;
(4) HORMONE TREATMENT: indole-3-butyric acid (IBA) and methyl α-naphthyl acetate (NAA) are mixed with hormone mixed solution, cuttings lower end 3 ~ 5cm is put into hormone mixed solution, soak 1h, pull out and slightly do that 2 ~ 3min is air-dry carries out cuttage immediately.Wherein, the compound method of hormone mixed solution is: first use the medicinal alcohol of 95wt.% indole-3-butyric acid (IBA) and methyl α-naphthyl acetate (NAA) to be dissolved respectively, then adding distil water is configured to the concentrate that concentration is 10g/L; Measure two kinds of solution by volume respectively, add the distilled water constant volume of 22/3 times after mixing, be configured to the hormone mixed solution of IBA and NAA.
Step 3, cuttage: comprise
(1) matrix is watered: before cuttage, matrix is evenly watered, and makes substrate water content reach 67 ~ 73%;
(2) cuttage: be ready in the nutritious bag of mixed-matrix by being inserted into through the cuttings of hormone mixed solution process, every bag of cuttings, cuttings face of blade upward, direction is consistent, the cuttage degree of depth is 3 ~ 5cm, then by the cutting medium compacting around cuttings, waters root-growing agent solution residual on 1 permeable double flushing blade immediately, make substrate water content reach more than 90%, make cuttings fully contact with cutting medium simultaneously.
Manage after step 4, cuttage: comprise
(1) build shading shed: plug the simple and easy shed of thin bamboo strips used for weaving built in both sides, seedbed, cover overlay, then add lid layer 60% shading screen, if nursery itself is provided with overhead cool canopy, then need not add a cover shading screen, evenly cover compression molding material film edge with earth under seedbed, prevent seedbed water loss;
(2) disease control: sprayed once sterilizing every 7 ~ 15 days with carbendazim 1000 times of liquid (1000 times of liquid is exactly the water of medicine than upper 1000 milliliters of 1 gram) after cuttage, prevent from growing miscellaneous bacteria, spray and select to carry out after the sun sets, water spray was stopped before half an hour to morning sunrise before spray medicine, after spray medicine, overlay film is arranged, continues to keep sternly sealing state;
(3) manage after taking root: after cuttage 60 days, namely the cuttings of 90% takes root, now overlay is removed, 60 ~ 70% are kept to shade, prevent from being exposed to the sun, manage until go out garden in conventional liquid manure mode, frequency of watering is to keep substrate water content more than 80% for principle, according to fine day, the actual conditions at cloudy day adjustment irrigation times and intensity, the final subprostrate sophora nursery stock obtaining high-quality.
The invention provides the application of method in subprostrate sophora artificial culture that above-mentioned utilization mixing Light media carries out subprostrate sophora epicormic branch cutting.
Relative to prior art, beneficial effect of the present invention has:
1, utilize mixing Light media to carry out the annual epicormic branch cutting of subprostrate sophora, have matrix light (for 50% of single-matrix weight), permeability is good, and the nursery stock amount of taking root is large, the obvious feature of lateral root development; After flourishing side root is conducive to planting, seedling root recovers rapidly, grows, be conducive to improving survival rate, having under the condition of shading, the whole year all can carry out, be not subject to the restriction in time and season, be conducive to high efficiency and the continuity of breeding, be convenient to large-scale production and the Application and Development of subprostrate sophora cottage propagation;
2, utilize mixing Light media to carry out the nursery stock of the annual softwood cutting propagation of subprostrate sophora compared with sowing nursery stock, after planting, primary growth speed obviously increases, and amount of growth is large, strong adaptability;
3, mixing Light media is utilized to carry out the method for subprostrate sophora softwood cutting propagation, have simple, effect high inexpensive, reproduction speed is fast, reproduction coefficient is high, the feature of maintenance excellent strain that can be good, can directly apply to actual production, has good economic benefit and social benefit.
Embodiment
Can be well understood to the present invention further by specific embodiments of the invention given below and comparing embodiment, but they not limitation of the invention.The part do not described in detail in specific embodiment and comparing embodiment adopts prior art, known technology means and professional standard to obtain.
Except as otherwise noted, the percentage adopted in the present invention is percetage by weight.
embodiment 1
Utilize mixing Light media to carry out a method for subprostrate sophora epicormic branch cutting, comprise the steps:
1, cutting bed prepares:
(1) cutting bed: select leeward on the sunny side, the place of good water permeability builds outdoor cutting bed, the length 6 ~ 10m of cutting bed, wide 1m, the rough sand that cuttage bed one deck 3 ~ 5cm is thick is rebasing;
(2) mix the preparation of Light media: for subsequent use with the peat composed of rotten mosses, red heart soil and the mixing of coconut palm chaff, as the mixing Light media of cuttage, Light media will be mixed and load in the nutritious bag of 8 × 12, and closely be placed in cutting bed; First 1 day of cuttage is that the potassium permanganate of 0.1% is to the disinfection of mixing Light media by concentration.Wherein, each volume components mixed in Light media matches well than being the peat composed of rotten mosses: red heart soil: coconut palm chaff=3:2:1; In the present embodiment, the peat composed of rotten mosses can be the forest peat composed of rotten mosses that Northeast Area of China gathers; Coconut palm chaff can be the coconut palm chaff fully become thoroughly decomposed according to a conventional method.
2, cuttings acquisition and processing:
(1) cuttings collection: during annual April, gather first lignified annual green branch of elite stand as cuttage cuttings, especially, branch base portion profit of scion-seed tree trying one's best clip robust growth without the annual green branch of damage by disease and insect, semi-lignified as cuttage cuttings; In the present embodiment, cuttage cuttings can be robust growth, annual green branch without damage by disease and insect, semi-lignified that elite stand gathers;
(2) prune cuttings: the cuttings fringe bar of semi-lignified being cut into 10 ~ 15cm, remove the blade of above about the 8cm of base portion, lower end angle, bud is close in upper end, flat mouth, ties with rubber band or thin cotton cord;
(3) disinfect: cuttings is neatly placed in container (such as plastic basin), soak with 0.5% carbendazim, sterilization 20min, taking-up clear water rinses stand-by;
(4) HORMONE TREATMENT: indole-3-butyric acid (IBA) and methyl α-naphthyl acetate (NAA) are mixed with hormone mixed solution, cuttings lower end 3 ~ 5cm is put into hormone mixed solution, soak 1h, pull out and slightly do that 2 ~ 3min is air-dry carries out cuttage immediately.
In the present embodiment, the compound method of hormone mixed solution is: first use the medicinal alcohol of 95wt.% indole-3-butyric acid (IBA) and methyl α-naphthyl acetate (NAA) to be dissolved respectively, then adding distil water is configured to the concentrate that concentration is 10g/L; Measure two kinds of solution by volume respectively, add the distilled water constant volume of 22/3 times after mixing, be configured to the hormone mixed solution of IBA and NAA.
3, cuttage:
Cuttage is carried out during annual April.
(1) matrix is watered: before cuttage, matrix is evenly watered, and makes substrate water content reach 67 ~ 70%;
(2) cuttage: be ready in the nutritious bag of mixed-matrix by being inserted into through the cuttings of hormone mixed solution process, every bag of cuttings, cuttings face of blade upward, direction is consistent, the cuttage degree of depth is 3 ~ 4cm, then by the cutting medium compacting around cuttings, waters root-growing agent solution residual on 1 permeable double flushing blade immediately, make substrate water content reach more than 90%, make cuttings fully contact with cutting medium simultaneously.
4, manage after cuttage:
(1) build shading shed: plug the simple and easy shed of thin bamboo strips used for weaving built in both sides, seedbed, cover overlay, then add lid layer 60% shading screen, if nursery itself is provided with overhead cool canopy, then need not add a cover shading screen, evenly cover compression molding material film edge with earth under seedbed, prevent seedbed water loss;
(2) disease control: sprayed once sterilizing every 7 days with carbendazim 1000 times of liquid (1000 times of liquid is exactly the water of medicine than upper 1000 milliliters of 1 gram) after cuttage, prevent from growing miscellaneous bacteria, spray and select to carry out after the sun sets, water spray was stopped before half an hour to morning sunrise before spray medicine, after spray medicine, overlay film is arranged, continues to keep sternly sealing state;
(3) manage after taking root: investigation in 60 days after cuttage, rooting rate is 95.8%; Average primary radical 8.7, the long 6.8cm of average root.Now, overlay is removed, 60 ~ 65% are kept to shade, prevent from being exposed to the sun, manage until go out garden in conventional liquid manure mode, frequency of watering, to keep substrate water content for principle more than 80%, according to fine day, the actual conditions at cloudy day adjustment irrigation times and intensity, finally obtains the subprostrate sophora nursery stock of high-quality.
embodiment 2
Utilize mixing Light media to carry out a method for subprostrate sophora epicormic branch cutting, comprise the steps:
1, cutting bed prepares:
(1) cutting bed: select leeward on the sunny side, the place of good water permeability builds outdoor cutting bed, the length 10 ~ 12m of cutting bed, wide 1.2m, under cutting bed, on stone, rough sand is rebasing;
(2) mix the preparation of Light media: for subsequent use with the peat composed of rotten mosses, red heart soil and the mixing of coconut palm chaff, as the mixing Light media of cuttage, Light media will be mixed and load in the nutritious bag of 8 × 12, and closely be placed in cutting bed; First 1 day of cuttage is that the potassium permanganate of 0.1% is to the disinfection of mixing Light media by concentration.Wherein, each volume components mixed in Light media matches well than being the peat composed of rotten mosses: red heart soil: coconut palm chaff=2:2:1; In the present embodiment, the peat composed of rotten mosses can be the forest peat composed of rotten mosses that Northeast Area of China gathers; Coconut palm chaff can be the coconut palm chaff fully become thoroughly decomposed according to a conventional method.
2, cuttings acquisition and processing:
(1) cuttings collection: during annual July, gather first lignified annual green branch of elite stand as cuttage cuttings, especially, branch base portion profit of scion-seed tree trying one's best clip robust growth without the annual green branch of damage by disease and insect, semi-lignified as cuttage cuttings; In the present embodiment, cuttage cuttings can be robust growth, annual green branch without damage by disease and insect, semi-lignified that elite stand gathers;
(2) prune cuttings: the cuttings fringe bar of semi-lignified being cut into 8 ~ 9cm, remove the blade of above about the 6cm of base portion, lower end angle, bud is close in upper end, flat mouth, ties with rubber band or thin cotton cord;
(3) disinfect: cuttings is neatly placed in container (such as plastic basin), soak with 0.5% carbendazim, sterilization 20min, taking-up clear water rinses stand-by;
(4) HORMONE TREATMENT: indole-3-butyric acid (IBA) and methyl α-naphthyl acetate (NAA) are mixed with hormone mixed solution, cuttings lower end 3.5cm is put into the hormone mixed solution of IBA and NAA, soak 1h, pull out and slightly do that 3min is air-dry carries out cuttage immediately.
In the present embodiment, the compound method of hormone mixed solution is: first use the medicinal alcohol of 95wt.% indole-3-butyric acid (IBA) and methyl α-naphthyl acetate (NAA) to be dissolved respectively, then adding distil water is configured to the concentrate that concentration is 10g/L; Measure two kinds of solution by volume respectively, add the distilled water constant volume of 22/3 times after mixing, be configured to the hormone mixed solution of IBA and NAA.
3, cuttage:
Cuttage is carried out during annual July.
(1) matrix is watered: before cuttage, matrix is evenly watered, and makes substrate water content reach 70 ~ 73%;
(2) cuttage: be ready in the nutritious bag of mixed-matrix by being inserted into through the cuttings of hormone mixed solution process, every bag of cuttings, cuttings face of blade upward, direction is consistent, the cuttage degree of depth is 5cm, then by the cutting medium compacting around cuttings, waters root-growing agent solution residual on 1 permeable double flushing blade immediately, make substrate water content reach more than 90%, make cuttings fully contact with cutting medium simultaneously.
4, manage after cuttage:
(1) build shading shed: plug the simple and easy shed of thin bamboo strips used for weaving built in both sides, seedbed, cover overlay, then add lid layer 60% shading screen, if nursery itself is provided with overhead cool canopy, then need not add a cover shading screen, evenly cover compression molding material film edge with earth under seedbed, prevent seedbed water loss;
(2) disease control: sprayed once sterilizing every 7 days with carbendazim 1000 times of liquid (1000 times of liquid is exactly the water of medicine than upper 1000 milliliters of 1 gram) after cuttage, prevent from growing miscellaneous bacteria, spray and select to carry out after the sun sets, water spray was stopped before half an hour to morning sunrise before spray medicine, after spray medicine, overlay film is arranged, continues to keep sternly sealing state;
(3) manage after taking root: investigation in 60 days after cuttage, rooting rate is 85.6%; Average primary radical 6.3, the long 5.1cm of average root.Now, overlay is removed, 75% is kept to shade, prevent from being exposed to the sun, manage until go out garden in conventional liquid manure mode, frequency of watering, to keep substrate water content for principle more than 80%, according to fine day, the actual conditions at cloudy day adjustment irrigation times and intensity, finally obtains the subprostrate sophora nursery stock of high-quality.
embodiment 3
Utilize mixing Light media to carry out a method for subprostrate sophora epicormic branch cutting, comprise the steps:
1, cutting bed prepares:
(1) cutting bed: select leeward on the sunny side, the place of good water permeability builds outdoor cutting bed, the long 10m of cutting bed, wide 1.2m, under cutting bed, on stone, rough sand is rebasing;
(2) mix the preparation of Light media: for subsequent use with the peat composed of rotten mosses, red heart soil and the mixing of coconut palm chaff, as the mixing Light media of cuttage, Light media will be mixed and load in the nutritious bag of 8 × 12, and closely be placed in cutting bed; First 1 day of cuttage is that the potassium permanganate of 0.1% is to the disinfection of mixing Light media by concentration.Wherein, each volume components mixed in Light media matches well than being the peat composed of rotten mosses: red heart soil: coconut palm chaff=2:2:2; In the present embodiment, the peat composed of rotten mosses can be the forest peat composed of rotten mosses that Northeast Area of China gathers; Coconut palm chaff can be the coconut palm chaff fully become thoroughly decomposed according to a conventional method.
2, cuttings acquisition and processing:
(1) cuttings collection: during annual February, gather first lignified annual green branch of elite stand as cuttage cuttings, especially, branch base portion profit of scion-seed tree trying one's best clip robust growth without the annual green branch of damage by disease and insect, semi-lignified as cuttage cuttings; In the present embodiment, cuttage cuttings can be robust growth, annual green branch without damage by disease and insect, semi-lignified that elite stand gathers;
(2) prune cuttings: the cuttings fringe bar of semi-lignified being cut into 13 ~ 18cm, remove the blade of above about the 8cm of base portion, lower end angle, bud is close in upper end, flat mouth, ties with rubber band or thin cotton cord;
(3) disinfect: cuttings is neatly placed in container (such as plastic basin), soak with 0.5% carbendazim, sterilization 18min, taking-up clear water rinses stand-by;
(4) HORMONE TREATMENT: indole-3-butyric acid (IBA) and methyl α-naphthyl acetate (NAA) are mixed with hormone mixed solution, cuttings lower end 3cm is put into the hormone mixed solution of IBA and NAA, soak 1h, pull out and slightly do that 2min is air-dry carries out cuttage immediately.
In the present embodiment, the compound method of hormone mixed solution is: first use the medicinal alcohol of 95wt.% indole-3-butyric acid (IBA) and methyl α-naphthyl acetate (NAA) to be dissolved respectively, then adding distil water is configured to the concentrate that concentration is 10g/L; Measure two kinds of solution by volume respectively, add the distilled water constant volume of 22/3 times after mixing, be configured to the hormone mixed solution of IBA and NAA.
3, cuttage:
Cuttage is carried out during annual February.
(1) matrix is watered: before cuttage, matrix is evenly watered, and makes substrate water content reach 70%;
(2) cuttage: be ready in the nutritious bag of mixed-matrix by being inserted into through the cuttings of hormone mixed solution process, every bag of cuttings, cuttings face of blade upward, direction is consistent, the cuttage degree of depth is 4 ~ 5cm, then by the cutting medium compacting around cuttings, waters root-growing agent solution residual on 1 permeable double flushing blade immediately, make substrate water content reach more than 90%, make cuttings and cutting medium combine closely simultaneously.
4, manage after cuttage:
(1) build shading shed: plug the simple and easy shed of thin bamboo strips used for weaving built in both sides, seedbed, cover overlay, then add lid layer 60% shading screen, if nursery itself is provided with overhead cool canopy, then need not add a cover shading screen, evenly cover compression molding material film edge with earth under seedbed, prevent seedbed water loss;
(2) disease control: sprayed once sterilizing every 7 days with carbendazim 1000 times of liquid (1000 times of liquid is exactly the water of medicine than upper 1000 milliliters of 1 gram) after cuttage, prevent from growing miscellaneous bacteria, spray and select to carry out after the sun sets, water spray was stopped before half an hour to morning sunrise before spray medicine, after spray medicine, overlay film is arranged, continues to keep sternly sealing state;
(3) manage after taking root: investigation in 60 days after cuttage, the cuttage rooting of 82.3%; Average primary radical 5.2, the long 4.5cm of average root.Now, overlay is removed, 60% is kept to shade, prevent from being exposed to the sun, manage until go out garden in conventional liquid manure mode, frequency of watering, to keep substrate water content for principle more than 80%, according to fine day, the actual conditions at cloudy day adjustment irrigation times and intensity, finally obtains the subprostrate sophora nursery stock of high-quality.
embodiment 4
Utilize mixing Light media to carry out a method for subprostrate sophora epicormic branch cutting, comprise the steps:
1, cutting bed prepares:
(1) cutting bed: select leeward on the sunny side, the place of good water permeability builds outdoor cutting bed, the long 10m of cutting bed, wide 1m, under cutting bed, stone is rebasing;
(2) mix the preparation of Light media: for subsequent use with the peat composed of rotten mosses, red heart soil and the mixing of coconut palm chaff, as the mixing Light media of cuttage, Light media will be mixed and load in the nutritious bag of 8 × 12, and closely be placed in cutting bed; First 1 day of cuttage is that the potassium permanganate of 0.1% is to the disinfection of mixing Light media by concentration.Wherein, each volume components mixed in Light media matches well than being the peat composed of rotten mosses: red heart soil: coconut palm chaff=3:2:1; In the present embodiment, the peat composed of rotten mosses can be the forest peat composed of rotten mosses that Northeast Area of China gathers; Coconut palm chaff can be the coconut palm chaff fully become thoroughly decomposed according to a conventional method.
2, cuttings acquisition and processing:
(1) cuttings collection: during annual November, gather first lignified annual green branch of elite stand as cuttage cuttings, especially, branch base portion profit of scion-seed tree trying one's best clip robust growth without the annual green branch of damage by disease and insect, semi-lignified as cuttage cuttings; In the present embodiment, cuttage cuttings can be robust growth, annual green branch without damage by disease and insect, semi-lignified that elite stand gathers;
(2) prune cuttings: the cuttings fringe bar of semi-lignified being cut into 15 ~ 18cm, remove the blade of above about the 8cm of base portion, lower end angle, bud is close in upper end, flat mouth, ties with rubber band or thin cotton cord;
(3) disinfect: cuttings is neatly placed in container (such as plastic basin), soak with 0.5% carbendazim, sterilization 15min, taking-up clear water rinses stand-by;
(4) HORMONE TREATMENT: indole-3-butyric acid (IBA) and methyl α-naphthyl acetate (NAA) are mixed with hormone mixed solution, cuttings lower end 3cm is put into the hormone mixed solution of IBA and NAA, soak 1h, pull out and slightly do that 2min is air-dry carries out cuttage immediately.
In the present embodiment, the compound method of hormone mixed solution is: first use the medicinal alcohol of 95wt.% indole-3-butyric acid (IBA) and methyl α-naphthyl acetate (NAA) to be dissolved respectively, then adding distil water is configured to the concentrate that concentration is 10g/L; Measure two kinds of solution by volume respectively, add the distilled water constant volume of 22/3 times after mixing, be configured to the hormone mixed solution of IBA and NAA.
3, cuttage:
Cuttage is carried out during annual February.
(1) matrix is watered: before cuttage, matrix is evenly watered, and makes substrate water content reach 73%;
(2) cuttage: be ready in the nutritious bag of mixed-matrix by being inserted into through the cuttings of hormone mixed solution process, every bag of cuttings, cuttings face of blade upward, direction is consistent, the cuttage degree of depth is 4 ~ 5cm, then by the cutting medium compacting around cuttings, waters root-growing agent solution residual on 1 permeable double flushing blade immediately, make substrate water content reach more than 90%, make cuttings and cutting medium combine closely simultaneously.
4, manage after cuttage:
(1) build shading shed: plug the simple and easy shed of thin bamboo strips used for weaving built in both sides, seedbed, cover overlay, then add lid layer 60% shading screen, if nursery itself is provided with overhead cool canopy, then need not add a cover shading screen, evenly cover compression molding material film edge with earth under seedbed, prevent seedbed water loss;
(2) disease control: sprayed once sterilizing every 7 days with carbendazim 1000 times of liquid (1000 times of liquid is exactly the water of medicine than upper 1000 milliliters of 1 gram) after cuttage, prevent from growing miscellaneous bacteria, spray and select to carry out after the sun sets, water spray was stopped before half an hour to morning sunrise before spray medicine, after spray medicine, overlay film is arranged, continues to keep sternly sealing state;
(3) manage after taking root: investigation in 60 days after cuttage, the cuttage rooting of 92.5%; Average primary radical 7.3, the long 6.1cm of average root.Now, overlay is removed, 60% is kept to shade, prevent from being exposed to the sun, manage until go out garden in conventional liquid manure mode, frequency of watering, to keep substrate water content for principle more than 80%, according to fine day, the actual conditions at cloudy day adjustment irrigation times and intensity, finally obtains the subprostrate sophora nursery stock of high-quality.
comparative example 1
The implementation process of this comparative example is substantially the same manner as Example 1, and its change is the component and the volume ratio proportioning that mix Light media.
When the peat composed of rotten mosses: red heart soil: during coconut palm chaff=5:1:1, the too much peat composed of rotten mosses causes part to burn seedling phenomenon, shows as cuttings base portion blackout, dehydration, dries up.Investigation in 60 days after cuttage, rooting rate is only 58.6%, and the branch major part of not taking root dries up.
When the peat composed of rotten mosses: red heart soil: during coconut palm chaff=1:5:1, too much red heart soil causes matrix permeability poor, ponding is serious, and occurs matrix substrate junction phenomena in the later stage.Investigation in 60 days after cuttage, rooting rate is 62.3%, and root system owes full, and side root is few.
comparative example 2
The process of this comparative example is substantially the same manner as Example 2, and its change is the moisture control of matrix before and after cuttage, and difference comes from the change of watering the matrix physical state that normal root water causes.
Before cuttage, matrix is evenly watered permeable, substrate water content is made to reach more than 90%, matrix fully infiltrates, through the cuttings of hormone mixed solution process be inserted into mixing Light media is housed nutritious bag in, because matrix fully infiltrates, there is the phenomenon of matrix leaching in cuttings, the generating portion cavity, position that cuttings is inserted when watering normal root water again after inserting, matrix attachment is poor.Corresponding, investigation in 60 days after cuttage, rooting rate is only 55.3%, and the branch of not taking root has part to dry up because base portion exposes.
Before cuttage, matrix is evenly watered, substrate water content is made to reach 40%, matrix still locates leather hard, when cuttings through the process of hormone mixed solution is inserted into the nutritious bag that mixing Light media is housed, because matrix is partially dry, hardness is large, semi-lignified spray is difficult to because of tenderer insert, and the phenomenon fractureed appears in part, affects cuttage efficiency and cuttings quality.Corresponding, investigation in 60 days after cuttage, due to the loss that the branch that partly fractures brings, rooting rate is only 75.6%.
comparative example 3
The process of this comparative example is substantially the same manner as Example 4, and its change is component and the concentration of hormone mixed solution.
The solution of independent employing ABT6 root-inducing powder configuration variable concentrations, investigation in 60 days after cuttage, rooting rate difference is very large, from 0 to 59.6% not etc.
Adopt ABT6 root-inducing powder and indole-3-butyric acid (IBA) by variable concentrations proportioning configuration hormone mixed solution, investigation in 60 days after cuttage, rooting rate difference is very large, from 13.5% to 62.5% not etc.
Adopt ABT6 root-inducing powder and methyl α-naphthyl acetate (NAA) by variable concentrations proportioning configuration hormone mixed solution, investigation in 60 days after cuttage, rooting rate difference is very large, from 28.5% to 52.6% not etc.
When adopting the concentration ratio of indole-3-butyric acid (IBA) and methyl α-naphthyl acetate (NAA) more than 10:1, investigation in 60 days after cuttage, (situation of taking root exists bigger difference), rooting rate is only up to 78.3%.
When adopting the concentration ratio of indole-3-butyric acid (IBA) and methyl α-naphthyl acetate (NAA) lower than 2:1, investigation in 60 days after cuttage, rooting rate is the highest only reaches 68.5%.
comparative example 4
The process of this comparative example is substantially the same manner as Example 4, and its change is that hormone mixed solution soaks the degree of depth of cuttings, and the cuttage degree of depth.
The degree of depth that hormone mixed solution soaks cuttings is 8cm, and when the cuttage degree of depth is 8cm, investigation in 60 days after cuttage, rooting rate is only up to 78.5%, and part branch carbonizes because excessive root-growing agent soaks bottom, dries up.
The degree of depth that hormone mixed solution soaks cuttings is 2cm, when the cuttage degree of depth is 2cm, investigation in 60 days after cuttage, rooting rate is only up to 68.3%, part branch does not have because hormone soaks insufficient sign of significantly taking root, and part branch is then crossed shallow water because of the cuttage degree of depth and divided under-supply and withered.
comparative example 5
The implementation process of this comparative example is substantially the same manner as Example 1, and its change is that the crop utilizing this Light media to cultivate is asiatic moonseed.
Within after finding cuttage 60 days, investigate, rooting rate is only 63.6%; Average primary radical 4.2, the long 4.5cm of average root.Now, overlay is removed, keep 75% to shade, prevent from being exposed to the sun, manage until go out garden in conventional liquid manure mode, frequency of watering is to keep substrate water content more than 80% for principle, according to fine day, the actual conditions at cloudy day adjustment irrigation times and intensity, final acquisition part asiatic moonseed nursery stock.Illustrate that Light media seedling medium of the present invention is not applicable to all plants.
The inventive method manages operation after comprising cutting bed preparation, cuttings acquisition and processing method, cuttage, cuttage, utilize the cuttage in Seedlings nursery of the annual spray of subprostrate sophora, by controlling illumination in Seedlings nursery, relative moisture and water content of substrate, final acquisition high-quality subprostrate sophora nursery stock.
The present invention mainly utilizes mixing Light media to carry out the annual epicormic branch cutting of subprostrate sophora, and have matrix light (for 50% of single-matrix weight), permeability is good, and the nursery stock amount of taking root is large, the obvious feature of lateral root development; After flourishing side root is conducive to planting, seedling root recovers rapidly, grows, and be conducive to improving survival rate, having under the condition of shading, the whole year all can carry out, and not by the restriction in time and season, is conducive to high efficiency and the continuity of breeding; And existing subprostrate sophora normal cutting propagation propagation method seedling medium weight is large; permeability is poor; the seedling-wood breeding cycle is long; rooting rate is unstable; root system is less-developed; main root is obvious, lateral root development is bad, and after afforestation, growth perfonnance is not good enough, is unfavorable for large-scale production and the Application and Development of subprostrate sophora cottage propagation.
The present invention utilizes mixing Light media to carry out the nursery stock of the annual softwood cutting propagation of subprostrate sophora compared with sowing nursery stock, and after planting, primary growth speed obviously increases, and amount of growth is large, strong adaptability; Therefore, subprostrate sophora softwood cutting propagation nursery stock is utilized to be more satisfactory afforestation material.
The present invention utilizes mixing Light media to carry out the method for subprostrate sophora softwood cutting propagation, have simple, effect high inexpensive, reproduction speed is fast, reproduction coefficient is high, the feature of maintenance excellent strain that can be good, can actual production be directly applied to, there is good economic benefit and social benefit.
Announced be only the preferred embodiments of the present invention above, certainly can not limit the interest field of the present invention with this, therefore according to the equivalent variations that the present patent application the scope of the claims is done, still belong to the scope that the present invention is contained.
Claims (10)
1. utilize mixing Light media to carry out a method for subprostrate sophora epicormic branch cutting, it is characterized in that: comprise the steps:
Step 1, cutting bed prepare: make cutting bed, lay stone and/or rough sand rebasing, configuration mixing Light media also loads in nutritious bag for subsequent use, and the component of described mixing Light media and volume ratio proportioning are the peat composed of rotten mosses: red heart soil: coconut palm chaff=(2-3): 2:(1-2);
Step 2, cuttings acquisition and processing: gather first lignified annual green branch of elite stand as cuttage cuttings, after pruning, sterilization, adopt hormone mixed solution immersion treatment;
Step 3, cuttage: matrix is evenly watered before cuttage, make substrate water content reach 67 ~ 73%, through the cuttings of hormone mixed solution process be inserted into mixing Light media is housed nutritious bag in, water immediately 1 time permeable to make substrate water content reach more than 90%;
Step 4, final-period management: the later stage carries out sheltering from heat or light and moisturizing process, manages until go out garden in conventional liquid manure mode, obtain the subprostrate sophora nursery stock of high-quality.
2. method according to claim 1, is characterized in that: in described step 1, select leeward on the sunny side, the place of good water permeability builds outdoor cutting bed, the stone that cuttage bed one deck 3 ~ 5cm is thick and/or rough sand rebasing.
3. method according to claim 1, is characterized in that: in described step 2, and described hormone closes the mixed aqueous solution that solution is indole-3-butyric acid and methyl α-naphthyl acetate very much.
4. method according to claim 1, is characterized in that: in described step 3, in first 1 day of cuttage with liquor potassic permanganate to the disinfection of mixing Light media.
5. method according to claim 1, is characterized in that: in described step 3, and cutting time is that annual March is to November.
6. method according to claim 1, is characterized in that: in described step 2 cuttings acquisition and processing, the fringe bar of semi-lignified is cut into the cuttings of 10 ~ 15cm; In step 3, cuttings lower end 3 ~ 5cm is put into hormone mixed solution and soaks; The cuttage degree of depth is 3 ~ 5cm.
7. method according to claim 1, is characterized in that: in described step 4 final-period management: build shading shed and add a cover shading screen and carry out process of sheltering from heat or light, cutting bed plastic covering film carries out moisturizing process.
8. method according to claim 1, is characterized in that: step 4 final-period management comprises disease control, sprays carbendazim carry out disinfection after cuttage every 7 ~ 15 days.
9. the application of method in subprostrate sophora artificial culture utilizing the mixing Light media of the peat composed of rotten mosses, red heart soil and coconut palm chaff to carry out subprostrate sophora epicormic branch cutting according to any one of claim 1 to 7.
10. application according to claim 9, is characterized in that, in the mixing Light media of the described peat composed of rotten mosses, red heart soil and coconut palm chaff, the volume ratio proportioning of each component is the peat composed of rotten mosses: red heart soil: coconut palm chaff=(2-3): 2:(1-2).
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107409925A (en) * | 2017-09-06 | 2017-12-01 | 广西壮族自治区林业科学研究院 | A kind of method of the asexual fast breeding of Moringa |
| CN107624534A (en) * | 2017-10-17 | 2018-01-26 | 伊犁师范学院 | A kind of method that Sophora alopecuroide seedling is efficiently bred using rhizome |
| CN109220271A (en) * | 2018-11-13 | 2019-01-18 | 富宁木洪恒林开发有限公司 | A kind of subprostrate sophora cuttage expanding propagation method |
-
2015
- 2015-12-10 CN CN201510915102.0A patent/CN105532395A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107409925A (en) * | 2017-09-06 | 2017-12-01 | 广西壮族自治区林业科学研究院 | A kind of method of the asexual fast breeding of Moringa |
| CN107624534A (en) * | 2017-10-17 | 2018-01-26 | 伊犁师范学院 | A kind of method that Sophora alopecuroide seedling is efficiently bred using rhizome |
| CN109220271A (en) * | 2018-11-13 | 2019-01-18 | 富宁木洪恒林开发有限公司 | A kind of subprostrate sophora cuttage expanding propagation method |
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