CN102630479A - Lavandula angustifolia Mill. cutting propagation method - Google Patents

Lavandula angustifolia Mill. cutting propagation method Download PDF

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Publication number
CN102630479A
CN102630479A CN2012101553120A CN201210155312A CN102630479A CN 102630479 A CN102630479 A CN 102630479A CN 2012101553120 A CN2012101553120 A CN 2012101553120A CN 201210155312 A CN201210155312 A CN 201210155312A CN 102630479 A CN102630479 A CN 102630479A
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cutting
cuttage
lavender
percentage
fringe
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CN2012101553120A
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Chinese (zh)
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孟林
田小霞
毛培春
陈淑燕
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Beijing Academy of Agriculture and Forestry Sciences
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Beijing Academy of Agriculture and Forestry Sciences
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Priority to CN2012101553120A priority Critical patent/CN102630479A/en
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Abstract

The invention relates to a lavandula angustifolia Mill. cutting propagation method. The invention relates to a cutting propagation method adopting the annual plants of lavandula angustifolia Mill. as mother ears, aiming at the present problems of low setting percentage, long seed germination and raised seedling propagation time, low survival percentage and the like of lavandula angustifolia Mill. The method comprises the steps of cutting medium preparation, cutting selection, rooting agent treatment, cutting, cutting culture conditions, management after cutting, transplanting and land management, wherein the cutting medium is optimized and the cuttings are subjected to single-cup single-plant cutting. The method has the following beneficial effects that the rooting percentage of the cultured cutting seedlings reaches 91%; the root systems are flourishing; and the transplanting survival percentage reaches 100%. Compared with lavandula angustifolia Mill. tissue culture and rapid propagation methods, the method has the advantages of simpleness in operation, small amount of labor, high growth speed, high rooting percentage, high transplanting survival percentage, robust seedlings and the like and has higher popularization and application values and vast development prospect.

Description

A kind of lavender cuttage breeding method
Technical field
The present invention relates to a kind of lavender cuttage breeding method, belong to field of horticultural production.
Background technology
Lavender ( Lavandula angustifoliaMill. ﹚ for the perennial undershrub of Labiatae (Labiatae), originates in Mediterranean Region and southern foot, Alps, from Turkey, inferior island, card there, the north, Africa, Spain distribution is arranged all to the Middle East.Mainly cultivate in countries such as France, Bulgaria, Italy, Britain.At present, the famous lavender place of production has two, lays respectively at the Provence of the French southeast and the Fu Liangye of Hokkaido, Japan.In Xinjiang of China is the main planting base of lavender, and its cultivated area accounts for more than 95% of the whole nation.Bright-coloured, the aromatic flavour of lavender color and luster; It is one of main natural perfume plant; Because of it is rich in essential oil and landscape effect is good; Not only be applied to industries such as pharmacy, spices and cosmetics, and be widely used in urban landscaping, ecological farm and the garden belonging to a category construction, demand is increasing.
At present, lavender is normal on producing adopts directly sowing to breed, but the seed-setting rate of lavender is low, and seed is less, and (thousand kernel weight is 0.9~1.0 g ﹚; It is hard to plant skin, keratinization, outsourcing wax; Germination is extremely slow and germination rate is extremely low, and there is dormancy in seed, so the seed propagation difficulty.Also there is report to utilize tissue culture to breed in recent years; Like notification number is that the Chinese patent of CN1586176A promptly discloses a kind of tissue culturing method for lavender, and this method adopts the method for solid-liquid suspensions-solid culture, cultivation lavender blade on the MS medium; The foundation callus suspension culture system rapidly of growing; The lavender seedling that regenerates at last obtains the lavender clone, and a kind of method of lavender rapid propagation in vitro is provided.Problems such as but the tissue culture propagating production cost is higher, and exists training tissue culture seedling difficult, and transplanting survival rate is relatively low.In order to enlarge lavender sapling multiplication quantity, save production cost, be convenient to simple operations, promote the fast development of plantation of lavender scale and processing, cottage propagation is a kind of effective technological means.The lavender cottage propagation can not only keep the merit of kind preferably, and adopts bar autumn and do not influence landscape effect and the extracts essential oil that lavender blooms, and Billy is with the more economical practicality of seminal propagation and feasible.
Summary of the invention
In view of this, high in order to overcome lavender tissue culture propagating production cost, and problem such as training tissue culture seedling is difficult, and transplanting survival rate is low, and then a kind of technical method of the lavender cottage propagation that operation is simple is provided.
The present invention provides a kind of lavender cuttage breeding method, and said method comprises step:
(1) preparation cutting medium: the peat composed of rotten mosses, vermiculite and garden mould mixed be mixed with cutting medium, every cubic metre of cutting medium is sterilized with carbendazim 40~50g mixing of percentage by weight 50%, in the nutrient cup of packing into;
(2) inserting ear selection gets: healthy and strong branch clip 7~9 cm that the selection maternal plant gives birth to semi-lignified then are at least with the slotting fringe of two joints, and said slotting fringe bottom is tiltedly cut 40 °~50 ° apart from 0.3~0.5 cm of joint portion, and top cuts flat mouth apart from the 0.3~0.5cm of joint portion;
(3) root-growing agent is handled: will insert 0~3 cm place, fringe lower end earlier with the disinfectant sterilization, and handle with root-inducing powder after cleaning with clear water again;
(4) cuttage: will insert the fringe lower end and insert in the cutting medium, the degree of depth is 2~3 cm;
(5) cuttage condition of culture: full sun the greenhouse, greenhouse daytime/night, mean temperature was 32 ℃ ± 3 ℃/25 ℃ ± 2 ℃, the daytime/night sky gas average relative humidity 75% ± 5%/82% ± 3%;
(6) insert the back management: water permeable and overlay film after the cuttage immediately, behind cuttage rooting, add forced ventilation gradually and increase light application time, remove film at last, remain not ponding of soil;
(7) transplant: select 1 year spring cloudy day or fine dusk to transplant, the cuttage seeding nutrient cup is divested transplanting to the pitting that digs from the bottom, tread behind the earthing, irrigate normal root water;
(8) field management: keep ground moistening.
The cutting medium of said preparation after the mixing sterilization, needs overlay film after 2~3 days usually, starts film, lets its medicine gas volatilization after 3-10 days, reinstalls in the nutrient cup.
Further, the said peat composed of rotten mosses: vermiculite: the volume ratio of garden mould is 2: 1: 1.
Further, said cuttage mode is single glass of individual plant cuttage.
Further, said root-growing agent treatment step is: at weight percent concentration 0.5%KMnO 4With said slotting fringe lower end sterilization 1~2 min, be soaked into 30 min in the ABT rooting powder solution of 1000ppm after cleaning with clear water again in the solution.
Further, said cutting time is that annual late September is to late October.
Cottage propagation generally carries out in spring, autumn, and summer, epicormic branch cuttage also could.The coarse sand of the medium of cuttage also available 2/3 mixes 1/3 peat moss.Select to grow healthy and vigorous breeding plant, choose the short and thick and annual semi-lignified branch terminal bud that do not ear of pitch, fringe is inserted in the intercepting of 8~10cm place in the top.The otch of slotting fringe is eustipes part closely, makes every effort to level and smooth, and phloem is broken.Not with the terminal bud cuttage that occurs inflorescence, because the branch of blooming is aging, can root of hair harmful effect growing way in future.
Beneficial effect of the present invention is:
(1) lavender is to be rich in essential oil and the good a kind of undershrub of landscape effect, and lavender cottage propagation technology of the present invention is simple to operate, and can effectively reduce seedling cost, raising nursery effect, additional income.Problems such as it is higher to have overcome its tissue culture propagating production cost, and the training tissue culture seedling difficulty, and transplanting survival rate is low.
(2) before the cuttage of the present invention to cutting medium carry out corresponding volume proportion (peat composed of rotten mosses: vermiculite: garden mould=2:1:1), both having possessed the characteristics of loose ventilative, light weight etc., have enough nutrient contents again, help it and take root, germinate and grow.
(3) insert fringe by single glass of individual plant cuttage, seedling percent is high, and rooting rate has reached 91%, and well developed root system, and transplanting survival rate reaches 100%, robust plant, and growing way is good.
(4) the present invention selects suitable seedling raise period; Promptly give birth to semi-lignified branch then in late September to the late October and the selection in every year; Neither influence the bloom landscape effect of period of lavender; Do not influence extracts essential oil again, than seminal propagation and the more economical practicality of tissue culture propagating and more feasible.
Embodiment
Below in conjunction with specific embodiment the present invention is described further, but protection scope of the present invention is not limited in this.
Embodiment 1:
This test is divided into invention group and control group; The invention group is the peat composed of rotten mosses: the volume ratio of vermiculite: garden mould=2:1:1 mixes as cutting medium; Control group is that garden mould is made cutting medium, is respectively charged in 50 8 cm * 8 cm plastics nutrient cup, handles 50 narrow leaf lavenders for every group and inserts fringe.In the greenhouse by solar heat of Beijing City Agriculture and Forestry Institute, implement.Choose and give birth to the slotting fringe of semi-lignified branch work then, every slotting spike length 7~9 cm are with two joints, and the bottom is tiltedly cut 45 °, and apart from 0.3~0.5 cm of joint portion, top cuts flat mouth, apart from 0.3~0.5 cm of joint portion; Every slotting fringe only keeps upper blade.
Insert 0~3cm place, fringe lower end and use 0.5%KMnO earlier 4Be soaked into 30 min in the ABT rooting powder solution of 1000ppm after solution disinfection 1~2 min, clear water clean again, after the taking-up, single glass of vertical insertion of slotting fringe individual plant be equipped with in the nutrient cup of matrix, the cuttage degree of depth 2~3 cm, water after slotting permeable, cover film.
Facility is greenhouse full sun, and greenhouse daytime/night, mean temperature was 32 ℃ ± 3 ℃/25 ℃ ± 2 ℃, the daytime/night sky gas average relative humidity 75% ± 5%/82% ± 3%.Insert the back management, weekly or watered once in ten days, look soil moisture content and decide, keep not ponding to be advisable.Afternoon note ventilating, and on film spray cooling.
The slotting fringe that narrow leaf lavender is organized in invention begins long callus after 10 days, remove film; Begin to take root after 30 days; Rooting rate reached more than 91% in 35~45 days, and transplant into the land for growing field crops survival rate and reach 100% 1 year spring.The cutting of the narrow leaf lavender of control group began long callus in 19 days, removed film; Begin to take root after 47 days, rooting rate has only reached to transplant into the land for growing field crops survival rate 75%, the second year spring and has reached 100% in the time of 60 days.Two groups of experimental value means measuring behind the cuttage 60d are seen table 1.
Embodiment 2:
In the greenhouse by solar heat of Beijing City Agriculture and Forestry Institute, implement.The semi-lignified branch of giving birth to then of wide leaf lavender of clip and Xinjiang lavender makes to insert fringe respectively, and each clip is inserted 50 of fringes.Every slotting spike length 7~9 cm are with two joints, and the bottom is tiltedly cut 45 °, and apart from 0.3~0.5 cm of joint portion, top cuts flat mouth, apart from 0.3~0.5 cm of joint portion; Every slotting fringe only keeps upper blade.
Insert 0~3cm place, fringe lower end and use 0.5%KMnO earlier 4After solution disinfection 1~2 min, clear water clean, be soaked into again in the ABT rooting powder solution of 1000ppm and take out behind 30 min.
Cutting medium is the peat composed of rotten mosses: the volume ratio of vermiculite: garden mould=2:1:1 mixes, in 8 cm that pack into * 8 cm plastics nutrient cup.
With inserting single glass of vertical matrix that mixes of inserting of fringe individual plant, the cuttage degree of depth 2~3 cm, water after inserting permeable, cover film.
Facility is greenhouse full sun, and greenhouse daytime/night, mean temperature was 32 ℃ ± 3 ℃/25 ℃ ± 2 ℃, the daytime/night sky gas average relative humidity 75% ± 5%/82% ± 3%.Insert the back management, weekly or watered once in 10 days, look soil moisture content and decide, keep not ponding to be advisable.Afternoon note ventilating, and on film spray cooling.
Lavender is transplanted a cutting and begins the callus of growing after 10 days, removes film; Begin to take root after 30 days; Xinjiang lavender rooting rate reached more than 95% in 35~45 days, and wide leaf lavender reaches more than 97%, and each index experimental value mean of measuring behind the cuttage 50d is seen table 2.Transplant into the land for growing field crops survival rate and reach 100% 1 year spring.
 

Claims (5)

1. a lavender cuttage breeding method is characterized in that, said method comprises step:
(1) preparation cutting medium: the peat composed of rotten mosses, vermiculite and garden mould mixed be mixed with cutting medium, every cubic metre of cutting medium is sterilized with carbendazim 40~50g mixing of percentage by weight 50%, in the nutrient cup of packing into;
(2) inserting ear selection gets: healthy and strong branch clip 7~9 cm that the selection maternal plant gives birth to semi-lignified then are at least with the slotting fringe of two joints, and said slotting fringe bottom is tiltedly cut 40 °~50 ° apart from 0.3~0.5 cm of joint portion, and top cuts flat mouth apart from 0.3~0.5 cm of joint portion;
(3) root-growing agent is handled: will insert 0~3 cm place, fringe lower end earlier with the disinfectant sterilization, and handle with root-inducing powder after cleaning with clear water again;
(4) cuttage: will insert the fringe lower end and insert in the cutting medium, the degree of depth is 2~3 cm;
(5) cuttage condition of culture: full sun the greenhouse, greenhouse daytime/night, mean temperature was 32 ℃ ± 3 ℃/25 ℃ ± 2 ℃, the daytime/night sky gas average relative humidity 75% ± 5%/82% ± 3%;
(6) insert the back management: water permeable and overlay film after the cuttage immediately, behind cuttage rooting, add forced ventilation gradually and increase light application time, remove film at last, remain not ponding of soil;
(7) transplant: select 1 year spring cloudy day or fine dusk to transplant, the cuttage seeding nutrient cup is divested transplanting to the pitting that digs from the bottom, tread behind the earthing, irrigate normal root water;
(8) field management: keep ground moistening.
2. lavender cuttage breeding method according to claim 1 is characterized in that, the said peat composed of rotten mosses: vermiculite: the volume ratio of garden mould is 2: 1: 1.
3. lavender cuttage breeding method according to claim 1 is characterized in that: said cuttage mode is single glass of individual plant cuttage.
4. lavender cuttage breeding method according to claim 1 is characterized in that: said root-growing agent treatment step is: at weight percent concentration 0.5%KMnO 4With said slotting fringe lower end sterilization 1~2 min, be soaked into 30 min in the ABT rooting powder solution of 1000ppm after cleaning with clear water again in the solution.
5. lavender cuttage breeding method according to claim 1 is characterized in that: said cutting time is that annual late September is to late October.
CN2012101553120A 2012-05-18 2012-05-18 Lavandula angustifolia Mill. cutting propagation method Pending CN102630479A (en)

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Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103766117A (en) * 2014-01-03 2014-05-07 新疆生产建设兵团第四师农业科学研究所 Cutting propagation method for lavender
CN103814718A (en) * 2014-02-11 2014-05-28 宋光辉 Cutting propagation method of lavender
CN104186292A (en) * 2014-09-11 2014-12-10 丹阳市世外桃源果品专业合作社 Cuttage planting method of lavender
CN104285642A (en) * 2014-09-30 2015-01-21 张家港市杨舍镇善港农民专业合作社 Planting method of lavender
CN105075609A (en) * 2015-08-20 2015-11-25 四川紫颐天堂香草科技开发有限公司 Cutting method for lavenders
CN105145103A (en) * 2015-10-14 2015-12-16 云南省农业科学院花卉研究所 Cutting seedling raising method of lavender in high attitude area
CN105248073A (en) * 2015-09-22 2016-01-20 江苏农林职业技术学院 Cutting propagation method of Zoysia matrella
CN106613268A (en) * 2016-12-30 2017-05-10 云南磷化集团有限公司 Method for quickly growing seedlings of lavender by light medium
CN107318415A (en) * 2017-06-30 2017-11-07 广西现代园林绿化工程种苗有限公司 A kind of cuttage and quick-propagation method of flower plants and nursery stock
CN107593130A (en) * 2017-10-10 2018-01-19 新疆天然芳香农业科技有限公司 Lavender epicormic branch cuttage method
CN108293560A (en) * 2017-10-18 2018-07-20 甘肃省治沙研究所 A method of rapidly and efficiently black fruit fructus lycii container cutting plant culture and afforestation
CN108967104A (en) * 2018-06-12 2018-12-11 浦江县元寿农业科技有限公司 Lavender method for culturing seedlings
CN109588123A (en) * 2019-01-11 2019-04-09 哈尔滨师范大学 A kind of extremely frigid zones lavender efficient breeding method
CN110366922A (en) * 2019-08-21 2019-10-25 伊犁哈萨克自治州农业科学研究所 The method of lavender cuttage and seedling culture

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Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103766117A (en) * 2014-01-03 2014-05-07 新疆生产建设兵团第四师农业科学研究所 Cutting propagation method for lavender
CN103766117B (en) * 2014-01-03 2015-09-23 新疆生产建设兵团第四师农业科学研究所 A kind of lavender cuttage breeding method
CN103814718A (en) * 2014-02-11 2014-05-28 宋光辉 Cutting propagation method of lavender
CN104186292A (en) * 2014-09-11 2014-12-10 丹阳市世外桃源果品专业合作社 Cuttage planting method of lavender
CN104285642A (en) * 2014-09-30 2015-01-21 张家港市杨舍镇善港农民专业合作社 Planting method of lavender
CN105075609A (en) * 2015-08-20 2015-11-25 四川紫颐天堂香草科技开发有限公司 Cutting method for lavenders
CN105248073A (en) * 2015-09-22 2016-01-20 江苏农林职业技术学院 Cutting propagation method of Zoysia matrella
CN105145103A (en) * 2015-10-14 2015-12-16 云南省农业科学院花卉研究所 Cutting seedling raising method of lavender in high attitude area
CN106613268A (en) * 2016-12-30 2017-05-10 云南磷化集团有限公司 Method for quickly growing seedlings of lavender by light medium
CN107318415A (en) * 2017-06-30 2017-11-07 广西现代园林绿化工程种苗有限公司 A kind of cuttage and quick-propagation method of flower plants and nursery stock
CN107593130A (en) * 2017-10-10 2018-01-19 新疆天然芳香农业科技有限公司 Lavender epicormic branch cuttage method
CN108293560A (en) * 2017-10-18 2018-07-20 甘肃省治沙研究所 A method of rapidly and efficiently black fruit fructus lycii container cutting plant culture and afforestation
CN108967104A (en) * 2018-06-12 2018-12-11 浦江县元寿农业科技有限公司 Lavender method for culturing seedlings
CN109588123A (en) * 2019-01-11 2019-04-09 哈尔滨师范大学 A kind of extremely frigid zones lavender efficient breeding method
CN110366922A (en) * 2019-08-21 2019-10-25 伊犁哈萨克自治州农业科学研究所 The method of lavender cuttage and seedling culture

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