CN103283340A - Method for promoting germination of cercidiphyllum japonicum seeds and increasing germination rate of cercidiphyllum japonicum seeds - Google Patents
Method for promoting germination of cercidiphyllum japonicum seeds and increasing germination rate of cercidiphyllum japonicum seeds Download PDFInfo
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Abstract
The invention discloses a method for promoting germination of cercidiphyllum japonicum seeds and increasing germination rate of the cercidiphyllum japonicum seeds. The method mainly comprises the steps of: selecting the cercidiphyllum japonicum seeds, disinfecting the cercidiphyllum japonicum seeds, laser-treating, breaking the dormancy of the cercidiphyllum japonicum seeds, germinating the cercidiphyllum japonicum seeds, and transplanting seedlings. According to the method, by comprehensively utilizing all aspects of laser treatment, vitriolization, hormone treatment, epilimnion stratification treatment, temperature and illumination intensity control, culture matrix selection and the like, the germination of the cercidiphyllum japonicum seeds is promoted, the germination rate is high; meanwhile, cercidiphyllum japonicum seed seedlings obtained by using the method are developed in root systems, good in growth vigour, high in speed and high in survival rate.
Description
Technical field
The present invention relates to a kind of method of breaking the katsura tree seed dormancy, promoting to sprout and improve seed germination rate.
Background technology
Katsura tree belongs to for the Cercidiphyllaceae katsura tree.Deciduous tree, katsura tree is distributed in Anhui, Zhejiang, Jiangxi, Hubei Province, river, Shan, sweet, Henan and southeast, Shanxi in China, and quantity is few, not anti-the moon, happiness is wet, is born in the other swale of mountain valley on the sunny side, ditch or shaw of 400 to 2700 meters of height above sea level more.Can both grow in neutrality, the acid ground.Area weather cold summer cold, most regional rainwater are more, high humidity.Katsura tree is Relict Plant in the Tertiary Period, and the relation for research tertiary plant fauna origin and China and Japanese plant's fauna has very important scientific research to be worth; It is a kind of ancient rare preciousness high megaphanerophyte of falling leaves, and trunk is perfectly straight, and the life-span is long, the tree performance grandness, and blade profile is peculiar attractive in appearance, therefore, is the very high afforestation seeds of ornamental value.Fruit can do medicinally with leaf, and leaf contains Jiao's property catechol; Cure mainly that child convulsion is twitched, limb is cold, have certain medical value; And katsura tree is precious in the world commerical tree species, and the grain of wood is perfectly straight, and structure is careful, and quality is hard, is filbert, can supply usefulness such as building, furniture, sleeper, engraving, joiner, also can be used for coinage; Bark and blade contain tannin, can obtain through refining tannin extract, and maltol contained in the leaf often is used to flavor potentiator in perfume industry, have very high economic worth.
Treelet need grow at sylvan life low light level place, and bearing tree requires certain illumination condition, poor growth, solid rareness.Because ripening rate is low, seedling is subject to harm such as heavy rain, damage by disease and insect, so the natural regeneration difficulty, the sylvan life treelet is few, and only felling is not over the years planted in addition, causes the area to dwindle gradually, and plant is more rare in flakes.As untimely protection, the katsura tree resource will be absorbed in the condition of extinction.It is to protect on the spot that the expert thinks on the one hand; strengthen the nature reserve management; actively take protection on the other hand with moving; at present existing many botanical gardens introducing and planting katsura tree; and obtained certain success, the more important thing is the reproduction rate that will improve katsura tree, the breeding of katsura tree at present mainly relies on seminal propagation; but the efficient of seminal propagation is very low under natural environment, and the expansion that has limited katsura tree so greatly is numerous.
Summary of the invention
The technical problem that the present invention mainly solves provides a kind of artificial technology of utilization and abolishes the seed dormant method of katsura tree, and takes certain measure to improve the seed germination rate of katsura tree.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is:
A kind of method that promotes the katsura tree seed germination and improve seed germination rate may further comprise the steps:
(1) choosing of seed: at first select the seed of the no damage by disease and insect of suitable locality for use, the seed of newly gathering is selected full grains, glossiness standby;
(2) sterilization of seed: seed cleans 2-3 time with warm water, and the liquor potassic permanganate of putting into 0.5-1% concentration then soaked 1-2 hour, is that the hydrogen peroxide of 5-10% was handled 2-3 hour with mass fraction again, filters the described thimerosal of removal then; Use the sterile water wash seed again 2-3 time, obtain sterile seed;
(3) laser treatment: the seed of sterilizing is dried the back naturally shine 4-10min at night with He-Ne laser biology irradiation instrument laser; Wherein He-Ne laser biology irradiation instrument is that Nanjing laser device factory produces, and wavelength is 632.8nm, and power density is 5mW/mm
2, spot diameter is 1.5mm.
(4) the broken dormancy of seed is handled: the katsura tree seed is soaked seed 3h at 40% sulfuric acid solution, behind flowing water flushing 24h, handled 1 day with the gibberellin that with mass fraction is 0.05% again, and then in 10-50 μ L/L salicylic acid solution, soaked 5-7 hour, put into cleaned container with the wet husky ratio according to 1:4 of preprepared through mixing then, cover with preservative film, and prick the breathing that several apertures are beneficial to seed at preservative film, the refrigerator of putting into 2-4 ℃ carries out low temperature lamination processing 20 days, take out sieve then and fall sand, thin layer dries in the shade, soak 22h with 0.2%ABA and 0.3%GA again, after moving to the husky 30d of Tibetan of 25 ℃ of thermostatic chambers, soak 12h with 0.4%ABA again, and then hid 30 days at 4 ℃ of low temperature sand;
(5) sprouting of seed: with treated seed, put into culture dish, bottom is spread double-deck filter paper in the culture dish, there is the culture matrix that configures in advance on the filter paper upper berth, it is positioned in the 22-25 ℃ of greenhouse then and carries out seed germination, per 3 d change filter paper 1 time, and per 5 d change culture dish No. 1 time, drip distilled water as required in right amount, keep the moisture of matrix at 40-50%; The control intensity of illumination is 1200-1400LX, and light application time is 12 hours/day, and temperature is 23-25 ℃, cultivates about 15 days, and seed sprouting bears seedling; The culture matrix that wherein said configured in advance is good is soil, sand and wheat bran, three's weight ratio 1:1:2.
(6) transplanting of seedling: with humus soil: pig manure: garden mould: the weight ratio mixed preparing matrix of vermiculite=4: 2: 1:2, tpn spray solution with 0.5% is on the matrix that stirs, carry out sterilization processing, keep 23-28 ℃ of temperature, relative moisture 70-75%, after 20-25 days, grow 10-20 centimetre seedling.
The invention has the beneficial effects as follows:
(1) utilize He-Ne laser biology irradiation instrument to carry out the laser irradiation, and control its irradiation time, like this can be a kind of optimal stimulation to its seed, can significantly improve the activity of the inner enzyme of seed self, impel rate of metabolism to accelerate, and then showing as germination vigor and germination rate raising, root is long elongated.
(2) in the seed treatment stage katsura tree seed is soaked with 40% sulfuric acid solution, this processing can be eliminated the inhibition of kind of skin to a certain extent, make seed fully absorb moisture, also increased simultaneously the gas permeability of exosper, reduced the restriction of oxygen exchange, make the oxidized decomposition of mortifier, strengthened merismatic activation capacity.
(3) in the seed germination stage, take suitable temperature and light treatment, germination rate, germination vigor and germination index all are improved.
(4) adopt rational culture matrix in seedling replanting, seedling growth is vigorous.
Embodiment
Below preferred embodiment of the present invention is described in detail, thereby so that advantages and features of the invention can be easier to be it will be appreciated by those skilled in the art that protection scope of the present invention is made more explicit defining.
Embodiment 1
A kind of method that promotes the katsura tree seed germination and improve seed germination rate, specific as follows:
At first select the seed of the no damage by disease and insect of suitable locality for use, the seed of newly gathering is selected full grains, glossiness standby; Then seed is cleaned 2 times with warm water, the liquor potassic permanganate of putting into 0.5% concentration then soaked 2 hours, was that 5% hydrogen peroxide was handled 3 hours with mass fraction again, filtered the described thimerosal of removal then; Use the sterile water wash seed again 2 times, obtain sterile seed;
The seed of sterilizing is dried the back naturally shine 4-10min at night with He-Ne laser biology irradiation instrument laser; Wherein He-Ne laser biology irradiation instrument is that Nanjing laser device factory produces, and wavelength is 632.8nm, and power density is 5mW/mm
2, spot diameter is 1.5mm.Then the katsura tree seed is soaked seed 3h at 40% sulfuric acid solution, behind flowing water flushing 24h, handled 1 day with the gibberellin that with mass fraction is 0.05% again, and then in 10 μ L/L salicylic acid solution, soaked 7 hours, put into cleaned container with the wet husky ratio according to 1:4 of preprepared through mixing then, cover with preservative film, and prick the breathing that several apertures are beneficial to seed at preservative film, the refrigerator of putting into 2 ℃ carries out low temperature lamination processing 20 days, take out sieve then and fall sand, thin layer dries in the shade, soak 22h with 0.2%ABA and 0.3%GA again, after moving to the husky 30d of Tibetan of 25 ℃ of thermostatic chambers, soak 12h with 0.4%ABA again, and then hid 30 days at 4 ℃ of low temperature sand;
With treated seed, put into culture dish, bottom is spread double-deck filter paper in the culture dish, there is the culture matrix that configures in advance on the filter paper upper berth, it is positioned in 22 ℃ of greenhouses then and carries out seed germination, per 3 d change filter paper 1 time, and per 5 d change culture dish No. 1 time, drip distilled water as required in right amount, keep the moisture of matrix 40%; The control intensity of illumination is 1200LX, and light application time is 12 hours/day, and temperature is 23 ℃, cultivates about 15 days, and seed sprouting bears seedling; The culture matrix that wherein said configured in advance is good is soil, sand and wheat bran, three's weight ratio 1:1:2.
With humus soil: pig manure: garden mould: the weight ratio mixed preparing matrix of vermiculite=4: 2: 1:2, the tpn spray solution with 0.5% carries out sterilization processing on the matrix that stirs, keep 23 ℃ of temperature, relative moisture 70% after 20-25 days, grows 10-20 centimetre seedling.
Through above-mentioned processing, the seed germination rate of katsura tree is 92%, and germination vigor is 90%, and germination index is 51.6, and plant percent is 90%.
Embodiment 2
A kind of method that promotes the katsura tree seed germination and improve seed germination rate, concrete operations are as follows:
At first select the seed of the no damage by disease and insect of suitable locality for use, the seed of newly gathering is selected full grains, glossiness standby; Seed cleans the liquor potassic permanganate of putting into 1% concentration for 3 times then with warm water and soaked 1 hour then, is that 10% hydrogen peroxide was handled 2 hours with mass fraction again, filters the described thimerosal of removal then; Use the sterile water wash seed again 3 times, obtain sterile seed;
The seed of sterilizing is dried the back naturally shine 4-10min at night with He-Ne laser biology irradiation instrument laser; Wherein He-Ne laser biology irradiation instrument is that Nanjing laser device factory produces, and wavelength is 632.8nm, and power density is 5mW/mm
2, spot diameter is 1.5mm.Then the katsura tree seed is soaked seed 3h at 40% sulfuric acid solution, behind flowing water flushing 24h, handled 1 day with the gibberellin that with mass fraction is 0.05% again, and then in 50 μ L/L salicylic acid solution, soaked 5 hours, put into cleaned container with the wet husky ratio according to 1:4 of preprepared through mixing then, cover with preservative film, and prick the breathing that several apertures are beneficial to seed at preservative film, the refrigerator of putting into 4 ℃ carries out low temperature lamination processing 20 days, take out sieve then and fall sand, thin layer dries in the shade, soak 22h with 0.2%ABA and 0.3%GA again, after moving to the husky 30d of Tibetan of 25 ℃ of thermostatic chambers, soak 12h with 0.4%ABA again, and then hid 30 days at 4 ℃ of low temperature sand;
With treated seed, put into culture dish, bottom is spread double-deck filter paper in the culture dish, there is the culture matrix that configures in advance on the filter paper upper berth, it is positioned in 25 ℃ of greenhouses then and carries out seed germination, per 3 d change filter paper 1 time, and per 5 d change culture dish No. 1 time, drip distilled water as required in right amount, keep the moisture of matrix 50%; The control intensity of illumination is 1400LX, and light application time is 12 hours/day, and temperature is 25 ℃, cultivates about 15 days, and seed sprouting bears seedling; The culture matrix that wherein said configured in advance is good is soil, sand and wheat bran, three's weight ratio 1:1:2.
With humus soil: pig manure: garden mould: the weight ratio mixed preparing matrix of vermiculite=4: 2: 1:2, the tpn spray solution with 0.5% carries out sterilization processing on the matrix that stirs, keep 28 ℃ of temperature, relative moisture 75% after 20-25 days, grows 10-20 centimetre seedling.
Through above-mentioned processing, the seed germination rate of katsura tree is 95%, and germination vigor is 93%, and germination index is 54.2, and plant percent is 95%.
Embodiment 3
A kind of method that promotes the katsura tree seed germination and improve seed germination rate, specific as follows:
At first select the seed of the no damage by disease and insect of suitable locality for use, the seed of newly gathering is selected full grains, glossiness standby; Seed cleans the liquor potassic permanganate of putting into 0.8% concentration after 3 times with warm water and soaked 1.5 hours then, is that 8% hydrogen peroxide was handled 2.5 hours with mass fraction again, filters the described thimerosal of removal then; Use the sterile water wash seed again 3 times, obtain sterile seed;
The seed of sterilizing is dried the back naturally shine 4-10min at night with He-Ne laser biology irradiation instrument laser; Wherein He-Ne laser biology irradiation instrument is that Nanjing laser device factory produces, and wavelength is 632.8nm, and power density is 5mW/mm
2, spot diameter is 1.5mm.Then the katsura tree seed is soaked seed 3h at 40% sulfuric acid solution, behind flowing water flushing 24h, handled 1 day with the gibberellin that with mass fraction is 0.05% again, and then in 30 μ L/L salicylic acid solution, soaked 6 hours, put into cleaned container with the wet husky ratio according to 1:4 of preprepared through mixing then, cover with preservative film, and prick the breathing that several apertures are beneficial to seed at preservative film, the refrigerator of putting into 3 ℃ carries out low temperature lamination processing 20 days, take out sieve then and fall sand, thin layer dries in the shade, soak 22h with 0.2%ABA and 0.3%GA again, after moving to the husky 30d of Tibetan of 25 ℃ of thermostatic chambers, soak 12h with 0.4%ABA again, and then hid 30 days at 4 ℃ of low temperature sand;
With treated seed, put into culture dish, bottom is spread double-deck filter paper in the culture dish, there is the culture matrix that configures in advance on the filter paper upper berth, it is positioned in 24 ℃ of greenhouses then and carries out seed germination, per 3 d change filter paper 1 time, and per 5 d change culture dish No. 1 time, drip distilled water as required in right amount, keep the moisture of matrix 45%; The control intensity of illumination is 1300LX, and light application time is 12 hours/day, and temperature is 24 ℃, cultivates about 15 days, and seed sprouting bears seedling; The culture matrix that wherein said configured in advance is good is soil, sand and wheat bran, three's weight ratio 1:1:2.
With humus soil: pig manure: garden mould: the weight ratio mixed preparing matrix of vermiculite=4: 2: 1:2, the tpn spray solution with 0.5% carries out sterilization processing on the matrix that stirs, keep 26 ℃ of temperature, relative moisture 72% after 20-25 days, grows 10-20 centimetre seedling.
Through above-mentioned processing, the seed germination rate of katsura tree is 93%, and germination vigor is 94%, and germination index is 53.8, and plant percent is 94%.
The above only is embodiments of the invention; be not so limit claim of the present invention; every equivalent structure or equivalent flow process conversion that utilizes description of the present invention to do; or directly or indirectly be used in other relevant technical fields, all in like manner be included in the scope of patent protection of the present invention.
Claims (2)
1. one kind promotes katsura tree seed germination and the method that improves seed germination rate, may further comprise the steps:
(1) choosing of seed: at first select the seed of the no damage by disease and insect of suitable locality for use, the seed of newly gathering is selected full grains, glossiness standby;
(2) sterilization of seed: seed cleans 2-3 time with warm water, and the liquor potassic permanganate of putting into 0.5-1% concentration then soaked 1-2 hour, is that the hydrogen peroxide of 5-10% was handled 2-3 hour with mass fraction again, filters the described thimerosal of removal then; Use the sterile water wash seed again 2-3 time, obtain sterile seed;
(3) laser treatment: the seed of sterilizing is dried the back naturally shine 4-10min at night with He-Ne laser biology irradiation instrument laser;
(4) the broken dormancy of seed is handled: the katsura tree seed is soaked seed 3h at 40% sulfuric acid solution, behind flowing water flushing 24h, handled 1 day with the gibberellin that with mass fraction is 0.05% again, and then in 10-50 μ L/L salicylic acid solution, soaked 5-7 hour, put into cleaned container with the wet husky ratio according to 1:4 of preprepared through mixing then, cover with preservative film, and prick the breathing that several apertures are beneficial to seed at preservative film, the refrigerator of putting into 2-4 ℃ carries out low temperature lamination processing 20 days, take out sieve then and fall sand, thin layer dries in the shade, soak 22h with 0.2%ABA and 0.3%GA again, after moving to the husky 30d of Tibetan of 25 ℃ of thermostatic chambers, soak 12h with 0.4%ABA again, and then hid 30 days at 4 ℃ of low temperature sand;
(5) sprouting of seed: with treated seed, put into culture dish, bottom is spread double-deck filter paper in the culture dish, there is the culture matrix that configures in advance on the filter paper upper berth, it is positioned in the 22-25 ℃ of greenhouse then and carries out seed germination, per 3 d change filter paper 1 time, and per 5 d change culture dish No. 1 time, drip distilled water as required in right amount, keep the moisture of matrix at 40-50%; The control intensity of illumination is 1200-1400LX, and light application time is 12 hours/day, and temperature is 23-25 ℃, cultivates about 15 days, and seed sprouting bears seedling;
(6) transplanting of seedling: with humus soil: pig manure: garden mould: the weight ratio mixed preparing matrix of vermiculite=4: 2: 1:2, tpn spray solution with 0.5% is on the matrix that stirs, carry out sterilization processing, keep 23-28 ℃ of temperature, relative moisture 70-75%, after 20-25 days, grow 10-20 centimetre seedling.
2. a kind of method that promotes the katsura tree seed germination and improve seed germination rate according to claim 1, the described culture matrix in the step (5) be native, sand and wheat bran, three's weight ratio 1:1:2.
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| CN103430657A (en) * | 2013-08-16 | 2013-12-11 | 苏州仁成生物科技有限公司 | Method for promoting seed germination of cercidiphyllum japonicum and improving germination ratio of seeds |
| CN103444396A (en) * | 2013-08-16 | 2013-12-18 | 苏州仁成生物科技有限公司 | Cultivation for promoting seed germination of cercidiphyllum japonicum and increasing seed germination rate |
| CN105284531A (en) * | 2015-10-30 | 2016-02-03 | 广西金神农生态农业科技开发有限公司 | "Or" seedling cultivation method |
| CN105284233A (en) * | 2015-02-04 | 2016-02-03 | 王振月 | Germination accelerating method of northeastern crowberry seeds |
| CN109983875A (en) * | 2019-04-11 | 2019-07-09 | 上海市农业科学院 | A method of improving vegetable seeds resistance |
| CN111264368A (en) * | 2020-02-22 | 2020-06-12 | 长江大学 | Cultivation method of Lianxiang seedlings |
| CN111631102A (en) * | 2020-07-07 | 2020-09-08 | 青海省农林科学院 | Cultivation method for quick breeding of green Chinese onions |
| CN111990010A (en) * | 2020-09-17 | 2020-11-27 | 甘肃省小陇山林业实验局林业科学研究所 | Method for promoting seed germination of cercidiphyllum japonicum and improving seed germination rate |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103430657A (en) * | 2013-08-16 | 2013-12-11 | 苏州仁成生物科技有限公司 | Method for promoting seed germination of cercidiphyllum japonicum and improving germination ratio of seeds |
| CN103444396A (en) * | 2013-08-16 | 2013-12-18 | 苏州仁成生物科技有限公司 | Cultivation for promoting seed germination of cercidiphyllum japonicum and increasing seed germination rate |
| CN105284233A (en) * | 2015-02-04 | 2016-02-03 | 王振月 | Germination accelerating method of northeastern crowberry seeds |
| CN105284233B (en) * | 2015-02-04 | 2017-12-29 | 王振月 | A kind of Northeastern crowberry seed accelerating germination method |
| CN105284531A (en) * | 2015-10-30 | 2016-02-03 | 广西金神农生态农业科技开发有限公司 | "Or" seedling cultivation method |
| CN109983875A (en) * | 2019-04-11 | 2019-07-09 | 上海市农业科学院 | A method of improving vegetable seeds resistance |
| CN109983875B (en) * | 2019-04-11 | 2021-04-06 | 上海市农业科学院 | Method for improving stress resistance of plant seeds |
| CN111264368A (en) * | 2020-02-22 | 2020-06-12 | 长江大学 | Cultivation method of Lianxiang seedlings |
| CN111264368B (en) * | 2020-02-22 | 2022-03-25 | 长江大学 | Cultivation method of Lianxiang seedlings |
| CN111631102A (en) * | 2020-07-07 | 2020-09-08 | 青海省农林科学院 | Cultivation method for quick breeding of green Chinese onions |
| CN111631102B (en) * | 2020-07-07 | 2021-12-14 | 青海省农林科学院 | Cultivation method for quick breeding of green Chinese onions |
| CN111990010A (en) * | 2020-09-17 | 2020-11-27 | 甘肃省小陇山林业实验局林业科学研究所 | Method for promoting seed germination of cercidiphyllum japonicum and improving seed germination rate |
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Application publication date: 20130911 |