CN111264368A - Cultivation method of Lianxiang seedlings - Google Patents
Cultivation method of Lianxiang seedlings Download PDFInfo
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- CN111264368A CN111264368A CN202010109517.XA CN202010109517A CN111264368A CN 111264368 A CN111264368 A CN 111264368A CN 202010109517 A CN202010109517 A CN 202010109517A CN 111264368 A CN111264368 A CN 111264368A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G23/00—Forestry
- A01G23/02—Transplanting, uprooting, felling or delimbing trees
- A01G23/04—Transplanting trees; Devices for grasping the root ball, e.g. stump forceps; Wrappings or packages for transporting trees
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Abstract
The invention discloses a cultivation method of Lianxiang seedlings, which comprises the following steps: germination of the Lianxiang seeds; after the Lianxiang seeds germinate and expand cotyledons, applying 1/3MS + 0.008-0.02 mg/LABT nutrient solution; when the seedlings grow to 2-3 cm, applying 1/2MS + 0.03-0.07 mg/LABT + 0.005-0.05 mg/LGA of nutrient solution; transplanting with coconut soil when the seedlings grow to 6-8 cm, wherein the nutrient solution is 1/2MS + 0.08-0.12 mg/LABT + 0.01-0.03 mg/LGA; after transplanting for 28-32 days, spraying an aqueous solution of 0.08-0.12 mg/LABT + 0.01-0.03 mg/LGA; spraying an aqueous solution of 0.3-0.7 mg/LNAA + 0.3-0.7 mg/L6-BA. The method improves germination rate, seedling survival rate and seedling quality of Lianxiang seed.
Description
Technical Field
The invention relates to the technical field of plant cultivation, in particular to a cultivation method of a Lianxiang seedling.
Background
The Lianxiang is a national secondary protection plant and has ornamental and medicinal values, but the natural reproduction rate of the Lianxiang tree is extremely low at present, and Lianxiang seeds do not have dormancy. The germination rate of the seeds of the coptis chinensis bunge can only reach about 20 percent at most. After the seedlings are transplanted, the seedlings are hardly survived due to the influence of external factors such as moisture, temperature and the like, and experiments show that the death rate of the seedlings in 3 months can reach more than 80-90% and the survival rate after one year is less than 1% under the condition of laboratory manual management, which explains that the seedlings are not grown under the large trees but the seeds of the trees are astonishing. The biological characteristic of the cercidiphyllum japonicum becomes the biggest obstacle to seedling culture of the cercidiphyllum japonicum.
Therefore, how to develop a cultivation method for cultivating the oriental paperbush which can obviously improve the germination rate of the oriental paperbush seeds, the seedling rate and the seedling quality is called as a technical problem to be solved urgently.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a cultivation method of the Lianxiang seedlings, which obviously improves the germination rate of Lianxiang seeds, the seedling survival rate of the seedlings and the seedling quality.
The invention is realized by the following steps:
the invention aims to provide a cultivation method of a Lianxiang seedling, which comprises the following steps:
s1, after germination and cotyledon unfolding of Lianxiang seeds, applying 1/3MS + 0.008-0.02 mg/LABT nutrient solution;
s2, after the seedlings grow to 2-3 cm, applying 1/2MS + 0.03-0.07 mg/LABT + 0.005-0.05 mg/LGA of a nutrient solution;
s3, transplanting with coconut soil when seedlings grow to 6-8 cm, wherein the nutrient solution used in the flowerpot is 1/2MS + 0.08-0.12 mg/LABT + 0.01-0.03 mg/LGA;
s4, stopping applying the nutrient solution after transplanting for 28-32 days, and spraying an aqueous solution of 0.08-0.12 mg/LABT + 0.01-0.03 mg/LGA;
and when the plant height of the seedlings of the oriental paperbush reaches 16-22 cm, spraying an aqueous solution of 0.3-0.7 mg/LNAA + 0.3-0.7 mg/L6-BA.
Preferably, the cultivation in the steps 1 and 2 is performed by using a cultivation device comprising a soil-based tank for placing the coconut soil and a water-based tank for placing an aqueous solution or a nutrient solution, and the lower part of the soil-based tank is arranged in the water-based tank. The incubation device of patent No. CN208079940U previously filed by the inventor is preferred.
Compared with the prior art, the invention has the following advantages and effects:
the cultivation method of the seedlings of the oriental paperbush provided by the invention obviously improves germination rate of oriental paperbush seeds, seedling survival rate of seedlings and seedling quality. Specifically, the method comprises the following steps:
the coconut soil matrix is adopted in the step 1: the coconut soil matrix is loose, breathable and strong in water retention capacity, is beneficial to root growth compared with the traditional matrix soil, and is high in viability and growth vigor. Coconut soil can keep proper moisture, has an important effect on the growth of seedling roots, and compared with the traditional matrix, once water shortage occurs, irreversible damage is caused.
Promoting the survival of seedlings in the step 2: as the seedlings with the fragrance are weak, the root system is weak, and the seedlings are easy to die due to poor soil air permeability, different nutrient solutions and hormones are adopted for treatment (1/3 MS + 0.008-0.02 mg/LABT is applied in S1, 1/2MS + 0.03-0.07 mg/LABT + 0.005-0.05 mg/LGA is applied in S2) at different stages of seedling development, so that the seedling root system development and nutrient absorption are facilitated, and the survival rate of the seedlings is improved.
Improving the transplanting survival rate in the step 3: the coconut soil is transplanted, the root system is not damaged, and sufficient root system growth space and time are provided for the seedlings, so that the transplanting survival rate is improved. After transplanting, different nutrient solutions and hormones are adopted for treatment (the nutrient solution used in S3 is 1/2MS + 0.08-0.12 mg/LABT + 0.01-0.03 mg/LGA; the nutrient solution is stopped being applied in S4, and the aqueous solution of 0.08-0.12 mg/LABT + 0.01-0.03 mg/LGA is sprayed), so that the transplanting survival rate is further improved.
Improving the seedling survival rate of the seedlings in the step 4: the requirements of nutrient substances and hormone are higher and higher along with the growth of seedlings, and the seedling survival rate can be obviously improved and the seedlings are robust by spraying the aqueous solution of 0.3-0.7 mg/LNAA + 0.3-0.7 mg/L6-BA.
Drawings
FIG. 1 is a photograph of germinating seeds, wherein FIG. 1A is a photograph of normal germinating seeds FIG. 1B-FIG. 1C are photographs of a decrease in the number of root hairs resulting from dehydration until disappearance after germination of the seeds, and FIG. 1D is a photograph of a loss of viability of a radicle and death of a seedling;
FIG. 2 is a picture of a surviving seedling after transplantation in example 1;
FIG. 3 is a photograph showing the seedlings of example 1, comparative example 14 and comparative example 15 after culturing.
Detailed Description
It will be appreciated by those skilled in the art that the following examples are illustrative of the invention only and should not be taken as limiting the scope of the invention. The examples do not specify particular techniques or conditions, and are performed according to the techniques or conditions described in the literature in the art or according to the product specifications. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available by purchase.
Example 1
The embodiment provides a cultivation method of seedlings with oriental paperbush, which comprises the following steps:
s1, after the Lianxiang seeds germinate and expand cotyledons, applying 1/3MS +0.01mg/LABT nutrient solution;
s2, after the seedlings grow to 2-3 cm, applying 1/2MS + 0.03-0.07 mg/LABT + 0.005-0.05 mg/LGA of a nutrient solution;
s3, transplanting the seedlings with coconut soil into a flowerpot when the seedlings grow to 6-8 cm, wherein the nutrient solution used in the flowerpot is 1/2MS + 0.08-0.12 mg/LABT + 0.01-0.03 mg/LGA;
s4, after the seedlings are transplanted into the flowerpot for 28-32 days, the roots can absorb nutrients from the soil, so that the application of nutrient solution is stopped, and 0.08-0.12 mg/LABT + 0.01-0.03 mg/LGA aqueous solution is sprayed;
the picture of the transplanted and survived seedling is shown in figure 2.
and when the plant height of the seedlings of the oriental paperbush reaches 16-22 cm, spraying an aqueous solution of 0.3-0.7 mg/LNAA + 0.3-0.7 mg/L6-BA. And irrigating roots once every 13-17 days, and spraying for 2-5 times.
Example 2
In this embodiment, the method for cultivating seedlings of oriental paperbush includes changing the nutrient solution applied in step S1 to 1/3MS +0.008 mg/LABT; the rest of the procedure was the same as in example 1.
Example 3
In this embodiment, the method for cultivating seedlings of oriental paperbush comprises the steps of changing the nutrient solution applied in step S1 to 1/3MS +0.02 mg/LABT; the rest of the procedure was the same as in example 1.
Example 4
In this embodiment, the method for cultivating Lianxiang seedlings is provided, wherein the nutrient solution applied in step S2 is changed to 1/2MS +0.05mg/LABT +0.005 mg/LGA; the rest of the procedure was the same as in example 1.
Example 5
In this embodiment, the nutrient solution applied in step S2 is changed to 1/2MS +0.05mg/LABT +0.05 mg/LGA; the rest of the procedure was the same as in example 1.
Example 6
In this embodiment, the method for cultivating seedlings with oriental paperbush includes changing the nutrient solution applied in step S2 to 1/2MS +0.03mg/LABT +0.01 mg/LGA; the rest of the procedure was the same as in example 1.
Example 7
In this embodiment, the method for cultivating seedlings with oriental paperbush includes changing the nutrient solution applied in step S2 to 1/2MS +0.07mg/LABT +0.01 mg/LGA; the rest of the procedure was the same as in example 1.
Example 8
In this embodiment, the method for cultivating seedlings with oriental paperbush includes changing the nutrient solution applied in step S3 to 1/2MS +0.08mg/LABT +0.01 mg/LGA; the rest of the procedure was the same as in example 1.
Example 9
In this embodiment, the method for cultivating seedlings with oriental paperbush includes changing the nutrient solution applied in step S3 to 1/2MS +0.12mg/LABT +0.03 mg/LGA; the rest of the procedure was the same as in example 1.
Example 10
This embodiment provides a method for cultivating seedlings with oriental paperbush, in step S4, spraying with an aqueous solution of 0.08mg/LABT +0.03 mg/LGA; the rest of the procedure was the same as in example 1.
Example 11
This embodiment provides a method for cultivating seedlings with oriental paperbush, in step S4, spraying with an aqueous solution of 0.12mg/LABT +0.01 mg/LGA; the rest of the procedure was the same as in example 1.
Example 12
The embodiment provides a cultivation method of Lianxiang seedlings, wherein in step 4, an aqueous solution of 0.7mg/LNAA +0.3mg/L6-BA is adopted for spraying; the rest of the procedure was the same as in example 1.
Example 13
The embodiment provides a cultivation method of Lianxiang seedlings, wherein in step 4, an aqueous solution of 0.3mg/LNAA +0.7mg/L6-BA is adopted for spraying; the rest of the procedure was the same as in example 1.
Comparative example 1
In the comparative example, a method for cultivating seedlings of Lianxiang was provided, in which the application of the nutrient solution in step S1 was changed to 1/3 MS; the rest of the procedure was the same as in example 1.
Comparative example 2
In the comparative example, a cultivation method of seedlings of Lianxiang was provided, in which the nutrient solution applied in step S1 was changed to 1/3MS +0.5 mg/LABT; the rest of the procedure was the same as in example 1.
Comparative example 3
In the comparative example, a cultivation method of seedlings with Lianxiang was provided, in which the nutrient solution applied in step S1 was changed to 1/3MS +0.1 mg/LABT; the rest of the procedure was the same as in example 1.
Comparative example 4 the comparative example was carried out in the same manner as example 1 except that no nutrient solution was added in step S1.
Comparative example 5
The present comparative example provides a method for cultivating seedlings of Lianxiang, the nutrient solution applied in step S2 is changed to 1/2MS +0.05 mg/LABT; the rest of the procedure was the same as in example 1.
Comparative example 6
The present comparative example provides a method for cultivating seedlings with Lianxiang, wherein the nutrient solution applied in step S2 is changed to 1/2MS +0.05mg/LABT +0.1 mg/LGA; the rest of the procedure was the same as in example 1.
Comparative example 7
The present comparative example provides a method for cultivating seedlings with Lianxiang, wherein the nutrient solution applied in step S2 is changed to 1/2MS +0.01mg/LABT +0.01 mg/LGA; the rest of the procedure was the same as in example 1.
Comparative example 8
The comparative example provides a cultivation method of Lianxiang seedlings, the nutrient solution applied in step S2 is changed to 1/2MS +0.09mg/LABT +0.01 mg/LGA; the rest of the procedure was the same as in example 1.
Comparative example 9
The present comparative example provides a method for cultivating seedlings with Lianxiang, wherein the nutrient solution applied in step S3 is changed to 1/2MS +0.05mg/LABT +0.005 mg/LGA; the rest of the procedure was the same as in example 1.
Comparative example 10
The present comparative example provides a method for cultivating seedlings with Lianxiang, wherein the nutrient solution applied in step S3 is changed to 1/2MS +0.15mg/LABT +0.05 mg/LGA; the rest of the procedure was the same as in example 1.
Comparative example 11
This comparative example provides a method of growing seedlings of Lianxiang, except for transplanting without coconut soil; the rest of the procedure was the same as in example 1.
Comparative example 12
The comparative example provides a cultivation method of Lianxiang seedlings, and step S4 is implemented by spraying 0.1mg/LABT aqueous solution; the rest of the procedure was the same as in example 1.
Comparative example 13
The comparative example provides a cultivation method of Lianxiang seedlings, spraying 0.02mg/LGA aqueous solution in step S4; the rest of the procedure was the same as in example 1.
Comparative example 14
The comparative example provides a cultivation method of seedlings with the oriental paperbush, and in the step 4, 0.5mg/LNAA aqueous solution is adopted for spraying; the rest of the procedure was the same as in example 1.
Comparative example 15
The comparative example provides a cultivation method of Lianxiang seedlings, and in the step 4, 0.5mg/LNAA +0.1mg/L6-BA aqueous solution is adopted for spraying; the rest of the procedure was the same as in example 1.
Test example 1
First, after germination and cotyledon unfolding of the Lianxiang seeds in S1, the height, root system and growth of the seedlings of examples 1 to 3 and comparative examples 1 to 4 were counted after 30 days by applying the nutrient solution, and the results are shown in Table 1.
TABLE 1
As can be seen from Table 1, the height, root system and growth vigor of the seedlings of examples 1 to 3 of the present invention were significantly improved as compared with those of comparative examples 1 to 4; and example 1 is the best example.
Secondly, in S2, after the seedlings grow to 2-3 cm, nutrient solution is applied, and after one month, the heights, root systems and growth vigor of the seedlings of examples 1-7 and comparative examples 1-8 are counted, and the results are shown in tables 2-3.
TABLE 2
TABLE 3
From tables 2 and 3, the nutrient solution 1/2MS + 0.03-0.07 mg/LABT + 0.005-0.05 mg/LGA applied in S2 is beneficial to the development and nutrient absorption of the root system of the seedling, so that the survival rate of the seedling is improved.
Test example 2
First, after 1 month of transplantation, the survival rates of the seedlings of examples 1, 8 to 9 and comparative examples 9 to 11 were counted, and the results are shown in Table 4.
TABLE 4
Compared with comparative examples 9-10, the transplanting survival rates of examples 1 and 8-9 are remarkably improved, which shows that the nutrient solution used in S3 has better effect of 1/2MS + 0.08-0.12 mg/LABT + 0.01-0.03 mg/LGA.
Compared with the comparative example 11, the transplanting survival rate of the example 1 is as high as 84.5%, which shows that the transplanting with coconut soil does not damage the root system, and sufficient root system growth space and time are provided for the seedlings, so that the transplanting survival rate is improved.
In the experimental process, the germination process of the Lianxiang seeds is very sensitive to moisture, and the seeds or the germinated seedlings can be irreversibly damaged when the seeds are slightly lack of water. FIG. 1A shows a normally germinated seed FIG. 1B-FIG. 1C shows a reduction in the number of root hairs resulting from dehydration after germination of the seed until disappearance, and FIG. 1D shows a loss of viability of the radicle and death of the seedling. In particular as germinated seeds: the number of root hairs is reduced, the root hairs fall off, the radicle is atrophied, the root tip is withered and turns yellow, no root hairs exist or the root hairs fall off and disappear; ungerminated seeds lose vigor and do not germinate again even when supplemented with moisture. Therefore, the coconut soil can keep proper moisture and has important effect on the growth of seedling roots.
Second, half a month after spraying in S4, the survival rate of transplanted seedlings of examples 1, 10 to 11 and comparative examples 12 to 13 was counted, and the results are shown in Table 5.
TABLE 5
| Group of | Survival rate of transplantation |
| Comparative example 12: 0.1mg/LABT | 64.4％ |
| Example 10: 0.08mg/LABT +0.03mg/LGA | 83.8％ |
| Example 1: 0.1mg/LABT +0.02mg/LGA | 84.5％ |
| Example 11: 0.12mg/LABT +0.01mg/LGA | 84.3％ |
| Comparative example 13: 0.02mg/LGA | 67.6％ |
Compared with comparative examples 12 to 13, the transplanting survival rates of examples 1 and 10 to 11 are significantly improved, which indicates that the nutrient solution used in S4 has a better effect of 0.08 to 0.12mg/LABT +0.01 to 0.03 mg/LGA.
Test example 3
Three months later from the start of spraying the aqueous solution in step 4, the seedling quality of the seedlings of examples 1, 12 to 13 and comparative examples 14 to 15 was counted on the same day, and the results are shown in FIG. 3 and Table 6.
TABLE 6
As can be seen from Table 6, spraying the water solution of 0.3-0.7 mg/LNAA + 0.3-0.7 mg/L6-BA on the survived seedlings of the Lianxiang (plant height 16-22 cm) can further improve the seedling quality.
The invention is not to be considered as limited to the particular embodiments shown, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (9)
1. The cultivation method of the Lianxiang seedlings is characterized by comprising the following steps of:
step 1, germination of Lianxiang seeds: uniformly scattering the coptidis seeds on the coconut soil, and keeping the coconut soil layer moist;
step 2, survival culture of the Lianxiang seedlings:
s1, after germination and cotyledon unfolding of Lianxiang seeds, applying 1/3MS + 0.008-0.02 mg/LABT nutrient solution;
s2, after the seedlings grow to 2-3 cm, applying 1/2MS + 0.03-0.07 mg/LABT + 0.005-0.05 mg/LGA of a nutrient solution;
step 3, transplanting:
s3, transplanting with coconut soil when seedlings grow to 6-8 cm, wherein the nutrient solution used in the flowerpot is 1/2MS + 0.08-0.12 mg/LABT + 0.01-0.03 mg/LGA;
s4, stopping applying the nutrient solution after transplanting for 28-32 days, and spraying an aqueous solution of 0.08-0.12 mg/LABT + 0.01-0.03 mg/LGA;
step 4, seedling culture of the Lianxiang seedlings:
and when the plant height of the seedlings of the oriental paperbush reaches 16-22 cm, spraying an aqueous solution of 0.3-0.7 mg/LNAA + 0.3-0.7 mg/L6-BA.
2. A cultivation method of seedlings of oriental paperbush as claimed in claim 1, wherein the germination temperature of the seeds of oriental paperbush is 20-30 ℃.
3. The method for cultivating seedlings of oriental paperbush according to claim 1, wherein the nutrient solution used in S1 is 1/3MS +0.01 mg/LABT.
4. A method for cultivating seedlings of Lianxiang as claimed in claim 1, wherein in S2, the nutrient solution used is 1/2MS +0.05mg/LABT +0.01 mg/LGA.
5. A method for cultivating seedlings of Lianxiang as claimed in claim 1, wherein the nutrient solution used in S3 is 1/2MS +0.1mg/LABT +0.02 mg/LGA.
6. A method for cultivating seedlings of oriental paperbush according to claim 1, wherein the aqueous solution used in S4 is 0.1mg/LABT +0.02 mg/LGA.
7. The cultivation method of seedlings with oriental paperbush as claimed in claim 1, wherein the aqueous solution used in step 4 is 0.5mg/LNAA +0.5 mg/L6-BA.
8. The cultivation method of the seedlings with the incense as claimed in claim 1, wherein the spraying is performed for 2-5 times in step 4 every 13-17 days after root irrigation.
9. The method for cultivating seedlings with Linxiang as claimed in claim 1, wherein the cultivation in steps 1 and 2 is carried out using cultivation equipment comprising a soil-based tank for placing the coconut soil and a water-based tank for placing an aqueous solution or a nutrient solution, the soil-based tank being built in the water-based tank at a lower portion thereof.
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