CN103430657A - Method for promoting seed germination of cercidiphyllum japonicum and improving germination ratio of seeds - Google Patents
Method for promoting seed germination of cercidiphyllum japonicum and improving germination ratio of seeds Download PDFInfo
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Abstract
The invention discloses a method for promoting seed germination of a cercidiphyllum japonicum and improving the germination rate of seeds. The method is mainly achieved through steps of seed selecting, seed disinfecting, laser treatment, seed dormancy breaking treatment, seed germination, seedling transplant and the like. According to the invention, laser treatment, vitriolization, hormone treatment, fluctuating temperature stratification and temperature and illumination intensity control are comprehensively utilized, the seed germination of the cercidiphyllum japonicum can be prompted from all aspects such as the selection of culture medium, the germination ratio is high, a seeding root system of the seed of the cercidiphyllum japonicum is prosperous, the growth vigor is excellent, the speed is fast, and the survival rate is high.
Description
Technical field
The present invention relates to a kind of method of breaking the katsura tree seed dormancy, promoting to sprout and improve seed germination rate.
Background technology
Katsura tree is the Cercidiphyllaceae Cercidiphyllum.Deciduous tree, katsura tree is distributed in Anhui ,Zhe, Gan,E,Chuan, Ji Jin southeast, ,Gan, Henan, Shan in China, and quantity is few, not anti-the moon, happiness is wet, is born in the other swale of mountain valley on the sunny side, ditch or shaw of 400 to 2700 meters of height above sea level more.In neutrality, acid ground, can grow.The area weather cold summer is cool, and most regional rainwater are more, high humidity.Katsura tree is Relict Plant in the Tertiary Period, and the relation for research tertiary plant fauna origin and China and Japanese plant's fauna, have very important scientific research to be worth; It is a kind of ancient rare preciousness high megaphanerophyte of falling leaves, and trunk is perfectly straight, and the life-span is long, the tree performance grandness, and blade profile is peculiar attractive in appearance, therefore, is the Landscape Trees that ornamental value is very high.It is medicinal that fruit and leaf can be done, and leaf is containing Jiao's property catechol; Cure mainly that child convulsion is twitched, limb is cold, there is certain medical value; And katsura tree is precious in the world commerical tree species, and the grain of wood is perfectly straight, and structure is careful, and quality is hard, is filbert, can, for building, furniture, sleeper, engraving, joiner etc., also can be used for coinage; Bark and blade, containing tannin, can be obtained through refining tannin extract, and maltol contained in leaf often is used to flavor potentiator in perfume industry, has very high economic worth.
Treelet need to grow at sylvan life low light level place, and bearing tree requires certain illumination condition, poor growth, solid rareness.Because ripening rate is low, seedling is subject to the harm such as heavy rain, damage by disease and insect, therefore the natural regeneration difficulty, the sylvan life treelet is few, and only felling over the years, do not plant in addition, causes area to dwindle gradually, and plant is more rare in flakes.As protection not in time, the katsura tree resource will be absorbed in the condition of extinction.It is to protect on the spot that the expert thinks on the one hand; strengthen the nature reserve management; actively take on the other hand in situ conservation; at present existing many botanical gardens introducing and planting katsura tree; and obtained certain success, the more important thing is the reproduction rate that will improve katsura tree, the breeding of katsura tree at present mainly relies on seminal propagation; but the efficiency of seminal propagation is very low under natural environment, the expansion that has so greatly limited katsura tree is numerous.
Summary of the invention
The technical problem that the present invention mainly solves is to provide a kind of artificial technology of utilization and abolishes the seed dormant method of katsura tree, and takes certain measure to improve the seed germination rate of katsura tree.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is:
A kind of method that promotes the katsura tree seed germination and improve seed germination rate comprises the following steps:
(1) choosing of seed: at first select the seed without damage by disease and insect of suitable locality, the seed newly gathered is selected to full grains, glossiness standby;
(2) sterilization of seed: seed cleans 2-3 time with warm water, and the liquor potassic permanganate of then putting into 0.5-1% concentration soaks 1-2 hour, then the hydrogen peroxide that is 5-10% with mass fraction processing 2-3 hour, then filters and removes described thimerosal; Use again sterile water wash seed 2-3 time, obtain sterile seed;
(3) laser treatment: use He-Ne laser biology irradiation instrument Ear Mucosa Treated by He Ne Laser Irradiation 4-10min after the seed of sterilization is dried naturally at night; Wherein He-Ne laser biology irradiation instrument is the production of Nanjing Laser apparatus factory, and wavelength is 632.8nm, and power density is 5mW/mm
2, spot diameter is that 1.5mm(should operate and do not relate to Tom Clancy Splinter Cell, sudden change, repeatability possesses by force practicality).
(4) the broken dormancy of seed is processed: the katsura tree seed is soaked to seed 3h at 40% sulfuric acid solution, after rinsing 24h by flowing water, with the gibberellin that is 0.05% with mass fraction, processed 1 day again, and then soak 5-7 hour in 10-50 μ L/L salicylic acid solution, then put into cleaned container with the wet husky ratio according to 1:4 of preprepared through mixing, with preservative film, cover, and prick the breathing that several apertures are beneficial to seed on preservative film, the refrigerator of putting into 2-4 ℃ carries out cold stratification 20 days, then take out and screen out sand, thin layer dries in the shade, soak 22h with 0.2%ABA and 0.3%GA again, after moving to the husky 30d of Tibetan of 25 ℃ of thermostatic chambers, soak 12h with 0.4%ABA again, and then hide 30 days at 4 ℃ of low temperature sand,
(5) sprouting of seed: by treated seed, put into culture dish, in culture dish, bottom spreads double-deck filter paper, there is the culture matrix configured in advance on the filter paper upper berth, then it is positioned in 22-25 ℃ of greenhouse and carries out seed germination, every 3 d change filter paper 1 time, and every 5 d change culture dish No. 1 time, drip in right amount as required distilled water, keep the moisture of matrix at 40-50%; Controlled light intensity is 1200-1400LX, and light application time is 12 hours/day, and temperature is 23-25 ℃, cultivates about 15 days, and seed sprouting, bear seedling; The culture matrix that wherein said configured in advance is good is soil, sand and wheat bran, three's weight ratio 1:1:2.
(6) transplanting of seedling: by humus soil: pig manure: garden mould: the weight ratio mixed preparing matrix of vermiculite=4: 2: 1:2, tpn spray solution with 0.5% is on the matrix stirred, carry out sterilization processing, keep temperature 23-28 ℃, relative moisture 70-75%, after 20-25 days, grow the seedling of 10-20 centimetre.
The invention has the beneficial effects as follows:
(1) utilize He-Ne laser biology irradiation instrument to carry out Ear Mucosa Treated by He Ne Laser Irradiation, and control its irradiation time, like this can with to its seed, be that (this operation does not relate to Tom Clancy Splinter Cell, sudden change in a kind of optimal stimulation, repeatability possesses by force practicality), can significantly improve the activity of the inner enzyme of seed self, impel rate of metabolism to accelerate, and then show as germination vigor and germination rate raising, root is long elongated.
(2) in the seed treatment stage, the katsura tree seed is soaked with 40% sulfuric acid solution, this processing can be eliminated the inhibition of kind of skin to a certain extent, make seed fully absorb moisture, also increased the gas permeability of exosper simultaneously, reduced the restriction of oxygen exchange, make the oxidized decomposition of mortifier, strengthened merismatic activation capacity.
(3) in the seed germination stage, take suitable temperature and light treatment, germination rate, germination vigor and germination index all are improved.
(4) adopt rational culture matrix in seedling replanting, seedling growth is vigorous.
Embodiment
Below preferred embodiment of the present invention is described in detail, thereby so that advantages and features of the invention can be easier to be it will be appreciated by those skilled in the art that, protection scope of the present invention is made to more explicit defining.
embodiment 1
A kind of method that promotes the katsura tree seed germination and improve seed germination rate, specific as follows:
At first select the seed without damage by disease and insect of suitable locality, the seed newly gathered is selected to full grains, glossiness standby; Then warm water for seed is cleaned 2 times, the liquor potassic permanganate of then putting into 0.5% concentration soaks 2 hours, then the hydrogen peroxide that is 5% with mass fraction processes 3 hours, then filters the described thimerosal of removal; Use again the sterile water wash seed 2 times, obtain sterile seed;
After being dried naturally, the seed of sterilization uses He-Ne laser biology irradiation instrument Ear Mucosa Treated by He Ne Laser Irradiation 4-10min at night; Wherein He-Ne laser biology irradiation instrument is the production of Nanjing Laser apparatus factory, and wavelength is 632.8nm, and power density is 5mW/mm
2, spot diameter is that 1.5mm(should operate and do not relate to Tom Clancy Splinter Cell, sudden change, repeatability possesses by force practicality).Then the katsura tree seed is soaked to seed 3h at 40% sulfuric acid solution, after rinsing 24h by flowing water, with the gibberellin that is 0.05% with mass fraction, processed 1 day again, and then soak 7 hours in 10 μ L/L salicylic acid solution, then put into cleaned container with the wet husky ratio according to 1:4 of preprepared through mixing, with preservative film, cover, and prick the breathing that several apertures are beneficial to seed on preservative film, the refrigerator of putting into 2 ℃ carries out cold stratification 20 days, then take out and screen out sand, thin layer dries in the shade, soak 22h with 0.2%ABA and 0.3%GA again, after moving to the husky 30d of Tibetan of 25 ℃ of thermostatic chambers, soak 12h with 0.4%ABA again, and then hide 30 days at 4 ℃ of low temperature sand,
By treated seed, put into culture dish, in culture dish, bottom spreads double-deck filter paper, there is the culture matrix configured in advance on the filter paper upper berth, then it is positioned in 22 ℃ of greenhouses and carries out seed germination, every 3 d change filter paper 1 time, and every 5 d change culture dish No. 1 time, drip in right amount as required distilled water, keep the moisture of matrix 40%; Controlled light intensity is 1200LX, and light application time is 12 hours/day, and temperature is 23 ℃, cultivates about 15 days, and seed sprouting, bear seedling; The culture matrix that wherein said configured in advance is good is soil, sand and wheat bran, three's weight ratio 1:1:2.
By humus soil: pig manure: garden mould: the weight ratio mixed preparing matrix of vermiculite=4: 2: 1:2, the tpn spray solution with 0.5%, on the matrix stirred, carries out sterilization processing, keep 23 ℃ of temperature, relative moisture 70%, after 20-25 days, grow the seedling of 10-20 centimetre.
Through above-mentioned processing, the seed germination rate of katsura tree is 92%, and germination vigor is 90%, and germination index is 51.6, and plant percent is 90%.
embodiment 2
A kind of method that promotes the katsura tree seed germination and improve seed germination rate, concrete operations are as follows:
At first select the seed without damage by disease and insect of suitable locality, the seed newly gathered is selected to full grains, glossiness standby; Then seed cleans with warm water the liquor potassic permanganate then put into 1% concentration for 3 times and soaks 1 hour, then the hydrogen peroxide that is 10% with mass fraction processes 2 hours, then filters the described thimerosal of removal; Use again the sterile water wash seed 3 times, obtain sterile seed;
After being dried naturally, the seed of sterilization uses He-Ne laser biology irradiation instrument Ear Mucosa Treated by He Ne Laser Irradiation 4-10min at night; Wherein He-Ne laser biology irradiation instrument is the production of Nanjing Laser apparatus factory, and wavelength is 632.8nm, and power density is 5mW/mm
2, spot diameter is that 1.5mm(should operate and do not relate to Tom Clancy Splinter Cell, sudden change, repeatability possesses by force practicality).Then the katsura tree seed is soaked to seed 3h at 40% sulfuric acid solution, after rinsing 24h by flowing water, with the gibberellin that is 0.05% with mass fraction, processed 1 day again, and then soak 5 hours in 50 μ L/L salicylic acid solution, then put into cleaned container with the wet husky ratio according to 1:4 of preprepared through mixing, with preservative film, cover, and prick the breathing that several apertures are beneficial to seed on preservative film, the refrigerator of putting into 4 ℃ carries out cold stratification 20 days, then take out and screen out sand, thin layer dries in the shade, soak 22h with 0.2%ABA and 0.3%GA again, after moving to the husky 30d of Tibetan of 25 ℃ of thermostatic chambers, soak 12h with 0.4%ABA again, and then hide 30 days at 4 ℃ of low temperature sand,
By treated seed, put into culture dish, in culture dish, bottom spreads double-deck filter paper, there is the culture matrix configured in advance on the filter paper upper berth, then it is positioned in 25 ℃ of greenhouses and carries out seed germination, every 3 d change filter paper 1 time, and every 5 d change culture dish No. 1 time, drip in right amount as required distilled water, keep the moisture of matrix 50%; Controlled light intensity is 1400LX, and light application time is 12 hours/day, and temperature is 25 ℃, cultivates about 15 days, and seed sprouting, bear seedling; The culture matrix that wherein said configured in advance is good is soil, sand and wheat bran, three's weight ratio 1:1:2.
By humus soil: pig manure: garden mould: the weight ratio mixed preparing matrix of vermiculite=4: 2: 1:2, the tpn spray solution with 0.5%, on the matrix stirred, carries out sterilization processing, keep 28 ℃ of temperature, relative moisture 75%, after 20-25 days, grow the seedling of 10-20 centimetre.
Through above-mentioned processing, the seed germination rate of katsura tree is 95%, and germination vigor is 93%, and germination index is 54.2, and plant percent is 95%.
embodiment 3
A kind of method that promotes the katsura tree seed germination and improve seed germination rate, specific as follows:
At first select the seed without damage by disease and insect of suitable locality, the seed newly gathered is selected to full grains, glossiness standby; Then seed cleans with warm water the liquor potassic permanganate of putting into 0.8% concentration after 3 times and soaks 1.5 hours, then the hydrogen peroxide that is 8% with mass fraction processes 2.5 hours, then filters the described thimerosal of removal; Use again the sterile water wash seed 3 times, obtain sterile seed;
After being dried naturally, the seed of sterilization uses He-Ne laser biology irradiation instrument Ear Mucosa Treated by He Ne Laser Irradiation 4-10min at night; Wherein He-Ne laser biology irradiation instrument is the production of Nanjing Laser apparatus factory, and wavelength is 632.8nm, and power density is 5mW/mm
2, spot diameter is that 1.5mm(should operate and do not relate to Tom Clancy Splinter Cell, sudden change, repeatability possesses by force practicality).Then the katsura tree seed is soaked to seed 3h at 40% sulfuric acid solution, after rinsing 24h by flowing water, with the gibberellin that is 0.05% with mass fraction, processed 1 day again, and then soak 6 hours in 30 μ L/L salicylic acid solution, then put into cleaned container with the wet husky ratio according to 1:4 of preprepared through mixing, with preservative film, cover, and prick the breathing that several apertures are beneficial to seed on preservative film, the refrigerator of putting into 3 ℃ carries out cold stratification 20 days, then take out and screen out sand, thin layer dries in the shade, soak 22h with 0.2%ABA and 0.3%GA again, after moving to the husky 30d of Tibetan of 25 ℃ of thermostatic chambers, soak 12h with 0.4%ABA again, and then hide 30 days at 4 ℃ of low temperature sand,
By treated seed, put into culture dish, in culture dish, bottom spreads double-deck filter paper, there is the culture matrix configured in advance on the filter paper upper berth, then it is positioned in 24 ℃ of greenhouses and carries out seed germination, every 3 d change filter paper 1 time, and every 5 d change culture dish No. 1 time, drip in right amount as required distilled water, keep the moisture of matrix 45%; Controlled light intensity is 1300LX, and light application time is 12 hours/day, and temperature is 24 ℃, cultivates about 15 days, and seed sprouting, bear seedling; The culture matrix that wherein said configured in advance is good is soil, sand and wheat bran, three's weight ratio 1:1:2.
By humus soil: pig manure: garden mould: the weight ratio mixed preparing matrix of vermiculite=4: 2: 1:2, the tpn spray solution with 0.5%, on the matrix stirred, carries out sterilization processing, keep 26 ℃ of temperature, relative moisture 72%, after 20-25 days, grow the seedling of 10-20 centimetre.
Through above-mentioned processing, the seed germination rate of katsura tree is 93%, and germination vigor is 94%, and germination index is 53.8, and plant percent is 94%.
(this operation does not relate to Tom Clancy Splinter Cell, sudden change to the foregoing is only embodiments of the invention; repeatability possesses by force practicality); not thereby limit the scope of the claims of the present invention; every equivalent structure or conversion of equivalent flow process that utilizes description of the present invention to do; or directly or indirectly be used in other relevant technical fields, all in like manner be included in scope of patent protection of the present invention.
Claims (2)
1. one kind promotes katsura tree seed germination and the method that improves seed germination rate, comprises the following steps:
(1) choosing of seed: at first select the seed without damage by disease and insect of suitable locality, the seed newly gathered is selected to full grains, glossiness standby;
(2) sterilization of seed: seed cleans 2-3 time with warm water, and the liquor potassic permanganate of then putting into 0.5-1% concentration soaks 1-2 hour, then the hydrogen peroxide that is 5-10% with mass fraction processing 2-3 hour, then filters and removes described thimerosal; Use again sterile water wash seed 2-3 time, obtain sterile seed;
(3) laser treatment: use He-Ne laser biology irradiation instrument Ear Mucosa Treated by He Ne Laser Irradiation 4-10min after the seed of sterilization is dried naturally at night;
(4) the broken dormancy of seed is processed: the katsura tree seed is soaked to seed 3h at 40% sulfuric acid solution, after rinsing 24h by flowing water, with the gibberellin that is 0.05% with mass fraction, processed 1 day again, and then soak 5-7 hour in 10-50 μ L/L salicylic acid solution, then put into cleaned container with the wet husky ratio according to 1:4 of preprepared through mixing, with preservative film, cover, and prick the breathing that several apertures are beneficial to seed on preservative film, the refrigerator of putting into 2-4 ℃ carries out cold stratification 20 days, then take out and screen out sand, thin layer dries in the shade, soak 22h with 0.2%ABA and 0.3%GA again, after moving to the husky 30d of Tibetan of 25 ℃ of thermostatic chambers, soak 12h with 0.4%ABA again, and then hide 30 days at 4 ℃ of low temperature sand,
(5) sprouting of seed: by treated seed, put into culture dish, in culture dish, bottom spreads double-deck filter paper, there is the culture matrix configured in advance on the filter paper upper berth, then it is positioned in 22-25 ℃ of greenhouse and carries out seed germination, every 3 d change filter paper 1 time, and every 5 d change culture dish No. 1 time, drip in right amount as required distilled water, keep the moisture of matrix at 40-50%; Controlled light intensity is 1200-1400LX, and light application time is 12 hours/day, and temperature is 23-25 ℃, cultivates about 15 days, and seed sprouting, bear seedling;
(6) transplanting of seedling: by humus soil: pig manure: garden mould: the weight ratio mixed preparing matrix of vermiculite=4: 2: 1:2, tpn spray solution with 0.5% is on the matrix stirred, carry out sterilization processing, keep temperature 23-28 ℃, relative moisture 70-75%, after 20-25 days, grow the seedling of 10-20 centimetre.
2. a kind of method that promotes the katsura tree seed germination and improve seed germination rate according to claim 1, the described culture matrix in step (5) be native, sand and wheat bran, three's weight ratio 1:1:2.
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| CN103444396A (en) * | 2013-08-16 | 2013-12-18 | 苏州仁成生物科技有限公司 | Cultivation for promoting seed germination of cercidiphyllum japonicum and increasing seed germination rate |
| CN104255113A (en) * | 2014-09-12 | 2015-01-07 | 陈小洁 | Method for breaking dormancy of aquilegia viridiflora seeds |
| CN104396661A (en) * | 2014-10-23 | 2015-03-11 | 周琪 | Pseudotsuga brevifolia seedling-raising method |
| CN104686351A (en) * | 2015-03-02 | 2015-06-10 | 刘祖英 | In-vitro rapid propagation method of cercidiphyllum japonicum |
| CN104813771A (en) * | 2015-04-17 | 2015-08-05 | 常州市禾香塑业有限公司 | Method for promoting michelia sphaerantha seed germination |
| CN104813774A (en) * | 2015-04-17 | 2015-08-05 | 常州市禾香塑业有限公司 | Method for promoting geranium wilfordii seed germination |
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| CN104255113A (en) * | 2014-09-12 | 2015-01-07 | 陈小洁 | Method for breaking dormancy of aquilegia viridiflora seeds |
| CN104396661A (en) * | 2014-10-23 | 2015-03-11 | 周琪 | Pseudotsuga brevifolia seedling-raising method |
| CN104686351A (en) * | 2015-03-02 | 2015-06-10 | 刘祖英 | In-vitro rapid propagation method of cercidiphyllum japonicum |
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| CN107771672A (en) * | 2017-11-08 | 2018-03-09 | 江苏省中国科学院植物研究所 | A kind of method for obtaining Hibiscus hamabo aseptic seedling |
| CN108617380A (en) * | 2018-04-11 | 2018-10-09 | 江苏省中国科学院植物研究所 | A method of improving blueberry preserved effect |
| CN110651558A (en) * | 2019-10-12 | 2020-01-07 | 咸宁市农业科学院 | Method for improving germination rate of ramie seeds |
| CN110651558B (en) * | 2019-10-12 | 2021-07-23 | 咸宁市农业科学院 | Method for improving germination rate of ramie seeds |
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