CN104255113B - One breaks the seed dormant method of garden columbine - Google Patents
One breaks the seed dormant method of garden columbine Download PDFInfo
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- CN104255113B CN104255113B CN201410466883.5A CN201410466883A CN104255113B CN 104255113 B CN104255113 B CN 104255113B CN 201410466883 A CN201410466883 A CN 201410466883A CN 104255113 B CN104255113 B CN 104255113B
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Abstract
The present invention relates to one and break the seed dormant method of garden columbine, mainly comprise seed selection, seed immersion treatment, plant leaching liquor soak and the step of Stratificated treatment, the present invention fully utilizes immersion, Stratificated treatment etc. and breaks seed dormant technology, substantially reduce the time of garden columbine seed germination, and the seedling from seed well developed root system of the garden columbine utilizing the present invention to obtain, and growing way is good, speed is fast, and survival rate is high.
Description
Technical field
What the present invention relates to is that one breaks the seed dormant method of garden columbine.
Background technology
Garden columbine, another name: ram's horn flower, Classification system: AquilegiaviridifloraPall. is the kind of under Ranunculaceae, aquilegia.Originate in Europe and North America.For herbaceos perennial, root manure is large, cylindrical, slightly reaches 1.5cm, simple or have minority branch, crust pitchy.Root leaf, leaf surface is glossy, and there is fine hair at the back side, and bloom 6, between July, usual deep blue purple color or white, flower pesticide is yellow, hyoscine.Be born in the roadside, mountain region of height above sea level 200-2300m, river bank or moist meadow.Be distributed in the ground such as northeast, North China and Shaanxi, Ningxia, Gansu, Qinghai.There is distribution Far-east Area of Russia.There is good ornamental value, also can be used as medicine.
Not about the report breaking garden columbine seed dormancy method in prior art.
Summary of the invention
The present invention is directed to existing deficiency to provide one and break the seed dormant method of garden columbine.
The present invention realizes above-mentioned purpose by the following method.
One breaks the seed dormant method of garden columbine, and the method comprises the following steps:
(1) seed collection: whole inflorescence, without the healthy and strong strain of damage by disease and insect, to be cut with scissors and dried by choosing when seed is fully ripe, filters out full seed and fully the seed of maturation is for subsequent use;
(2) be first that 50g/L-60g/L potassium dihydrogen phosphate soaks after 15-18h by temperature concentration by garden columbine seed, put into thiophanate methyl 800 times of liquid and soak 2.5 hours, finally clean seed with the warm water of about 28 DEG C;
(3) plant leaching liquor is prepared: choose well-developed plant, gather root, stem, the leaf of vetiver, shred with scissors after drying in the shade, take respectively root, stem, leaf 2,10,4g, add 100 times of distilled water, 25 ~ 30 DEG C are soaked 48h, obtain leaching liquor stoste after 4 layers of filtered through gauze, obtain concentrate with Rotary Evaporators reduction vaporization again, be settled to 100ml; Add 20ppm gibberellin in above-mentioned and 15ppm salicylic acid obtains leaching liquor, the seed that step (2) obtains is soaked 5-7h in above-mentioned leaching liquor;
(4) to mix with the seed that step (3) obtains with sand, vermiculite, rice chaff and load seed packet, then seed packet is put into insulating box, temperature is set to 20-22 DEG C, Stratificated treatment 7-9d, spray a tpn 700 times of liquid every day period, while for matrix sterilization, soil humidity is made to remain on about 60%; In described Stratificated treatment, the mixing quality ratio of each composition is sand: vermiculite: rice chaff: seed=3:1:1:1.
Beneficial effect:
1, the present invention fully utilizes immersion, growth regulator controls, plant extraction liquid and Stratificated treatment etc. break seed dormant technology, and the seedling from seed well developed root system of the garden columbine utilizing the present invention to obtain, and growing way is good, and speed is fast, and survival rate is high.
2, after containing vetiver leaching liquor and growth regulator, salicylic immersion, the sprouting survival rate of garden columbine seed is greatly improved
3, present invention employs Stratificated treatment, comprise and the matrix of Stratificated treatment is reasonably configured, wherein rice chaff and river sand ensure that the gas permeability of matrix, sand is then for matrix maintains sufficient moisture, adopt above-mentioned setting, finally improve the emergence rate of garden columbine, ensure that the uniformity of emerging.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated, and following each embodiment, only for illustration of the present invention, does not limit the present invention.
Embodiment one
One breaks the seed dormant method of garden columbine, and the method comprises the following steps:
(1) seed collection: whole inflorescence, without the healthy and strong strain of damage by disease and insect, to be cut with scissors and dried by choosing when seed is fully ripe, filters out full seed and fully the seed of maturation is for subsequent use;
(2) be first that 50g/L potassium dihydrogen phosphate soaks after 15h by temperature concentration by garden columbine seed, put into thiophanate methyl 800 times of liquid and soak 2.5 hours, finally clean seed with the warm water of about 28 DEG C;
(3) plant leaching liquor is prepared: choose well-developed plant, gather root, stem, the leaf of vetiver, shred with scissors after drying in the shade, take respectively root, stem, leaf 2,10,4g, add 100 times of distilled water, 25 DEG C are soaked 48h, obtain leaching liquor stoste after 4 layers of filtered through gauze, obtain concentrate with Rotary Evaporators reduction vaporization again, be settled to 100ml; Add 20ppm gibberellin in above-mentioned and 15ppm salicylic acid obtains leaching liquor, the seed that step (2) obtains is soaked 5h in above-mentioned leaching liquor;
(4) to mix with the seed that step (3) obtains with sand, vermiculite, rice chaff and load seed packet, then seed packet is put into insulating box, temperature is set to 20 DEG C, Stratificated treatment 7d, spray a tpn 700 times of liquid every day period, while for matrix sterilization, soil humidity is made to remain on about 60%; In described Stratificated treatment, the mixing quality ratio of each composition is sand: vermiculite: rice chaff: seed=3:1:1:1.
Embodiment two
One breaks the seed dormant method of garden columbine, it is characterized in that the method comprises the following steps:
(1) seed collection: whole inflorescence, without the healthy and strong strain of damage by disease and insect, to be cut with scissors and dried by choosing when seed is fully ripe, filters out full seed and fully the seed of maturation is for subsequent use;
(2) be first that 60g/L potassium dihydrogen phosphate soaks after 18h by temperature concentration by garden columbine seed, put into thiophanate methyl 800 times of liquid and soak 2.5 hours, finally clean seed with the warm water of about 28 DEG C;
(3) plant leaching liquor is prepared: choose well-developed plant, gather root, stem, the leaf of vetiver, shred with scissors after drying in the shade, take respectively root, stem, leaf 2,10,4g, add 100 times of distilled water, 30 DEG C are soaked 48h, obtain leaching liquor stoste after 4 layers of filtered through gauze, obtain concentrate with Rotary Evaporators reduction vaporization again, be settled to 100ml; Add 20ppm gibberellin in above-mentioned and 15ppm salicylic acid obtains leaching liquor, the seed that step (2) obtains is soaked 7h in above-mentioned leaching liquor;
(4) to mix with the seed that step (3) obtains with sand, vermiculite, rice chaff and load seed packet, then seed packet is put into insulating box, temperature is set to 22 DEG C, Stratificated treatment 7-9d, spray a tpn 700 times of liquid every day period, while for matrix sterilization, soil humidity is made to remain on about 60%; In described Stratificated treatment, the mixing quality ratio of each composition is sand: vermiculite: rice chaff: seed=3:1:1:1.
Through measuring, after embodiment 1,2 processes, the germination rate of garden columbine reaches 95% respectively, and 97%.
Claims (1)
1. break the seed dormant method of garden columbine, it is characterized in that the method comprises the following steps:
(1) seed collection: whole inflorescence, without the healthy and strong strain of damage by disease and insect, to be cut with scissors and dried by choosing when seed is fully ripe, filters out full seed and fully the seed of maturation is for subsequent use;
(2) be first that 50g/L-60g/L potassium dihydrogen phosphate soaks after 15-18h by concentration by garden columbine seed, put into thiophanate methyl 800 times of liquid and soak 2.5 hours, finally clean seed with the warm water of 28 DEG C;
(3) plant leaching liquor is prepared: choose well-developed plant, gather root, stem, the leaf of vetiver, shred with scissors after drying in the shade, take respectively root, stem, leaf 2,10,4g, add 100 times of distilled water, 25 ~ 30 DEG C are soaked 48h, obtain leaching liquor stoste after 4 layers of filtered through gauze, obtain concentrate with Rotary Evaporators reduction vaporization again, be settled to 100ml; Add 20ppm gibberellin in above-mentioned and 15ppm salicylic acid obtains leaching liquor, the seed that step (2) obtains is soaked 5-7h in above-mentioned leaching liquor;
(4) to mix with the seed that step (3) obtains with sand, vermiculite, rice chaff and load seed packet, then seed packet is put into insulating box, temperature is set to 20-22 DEG C, Stratificated treatment 7-9d, spray a tpn 700 times of liquid every day period, while for matrix sterilization, soil humidity is made to remain on 60%; In described Stratificated treatment, the mixing quality ratio of each composition is sand: vermiculite: rice chaff: seed=3:1:1:1.
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| CN201410466883.5A CN104255113B (en) | 2014-09-12 | 2014-09-12 | One breaks the seed dormant method of garden columbine |
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| CN201410466883.5A CN104255113B (en) | 2014-09-12 | 2014-09-12 | One breaks the seed dormant method of garden columbine |
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| CN104255113B true CN104255113B (en) | 2016-01-06 |
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| CN108668558A (en) * | 2018-04-19 | 2018-10-19 | 边学静 | A method of abolishing Cephalotaxus mannii seed dormancy |
| CN109089758A (en) * | 2018-07-31 | 2018-12-28 | 安徽千草源生态农业开发有限公司 | A kind of high yield bletilla striata implantation methods |
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| CN102428810A (en) * | 2011-09-14 | 2012-05-02 | 杜品 | Breeding method of Rhodiola kirilowii |
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Inventor after: Cao Yanchun Inventor after: Li Ning Inventor after: Si Zhiguo Inventor after: Wang Zhenchao Inventor after: Zhang Junye Inventor after: Hu Jiguang Inventor after: Xia Bing Inventor before: Chen Xiaojie |
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Effective date of registration: 20151214 Address after: 450046 Henan city of Zhengzhou province Zheng Dong New District Longzihu College Park Ji Cheng Lu Applicant after: Henan Profession Technology College Address before: The East Ring Road in Liuzhou city 545000 No. 145 the Guangxi Zhuang Autonomous Region Lushan garden E district nine building 2-101 Applicant before: Chen Xiaojie |
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