CN104813773A - Method for promoting michelia shiluensis seed germination - Google Patents
Method for promoting michelia shiluensis seed germination Download PDFInfo
- Publication number
- CN104813773A CN104813773A CN201510183070.XA CN201510183070A CN104813773A CN 104813773 A CN104813773 A CN 104813773A CN 201510183070 A CN201510183070 A CN 201510183070A CN 104813773 A CN104813773 A CN 104813773A
- Authority
- CN
- China
- Prior art keywords
- seed
- michelia
- shiluensis
- soaked
- clean
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention relates to a method for promoting michelia shiluensis seed germination. The method includes stratification, dense sulfuric acid treatment, sodium bicarbonate treatment, plant clastic enzyme treatment, 6-BA treatment, calcium chloride treatment and plant extract treatment. The germination rate of michelia shiluensis seeds can be increased by 60%, germination uniformity can be remarkably improved, and the method has a wide application prospect and is of great significance for promoting cultivation of michelia shiluensis new varieties and stock reproduction.
Description
Technical field
The present invention relates to the method for plant seed germination, be specifically related to a kind of method promoting Michelia shiluensis seed germination.
Background technology
Michelia shiluensis is Magnoliaceae Michelia aiphyllium, and the narrow ellipse of terminal bud, is had light by orange-yellow or grey, the pubescence in pool.Sprig, leaf, petiole are all without hair.Leaf keratin, slightly hard, the shape of falling ovum Long Circle, long 8-14 (20) centimetre, wide 4-7(8) centimetre, tip circle blunt, the short point of tool, base portion wedge shape or wide wedge shape, above bottle green, lower flour is green, without hair, and lateral vein every limit 8-12 bar, after net arteries and veins is dry, two sides is all protruding; The long 1-3 centimetre of petiole, the wide ditch of tool, without stipule trace.Floral white, tepal 9 pieces, 3 take turns, obovate, long 3-4.5 centimetre, wide 1.5-2.5 centimetre; The long 2-2.5 centimetre of stamen, filigree is red; The long 1.4-2.1 centimetre of gynoecium, by micro-pubescence; Carpel oval, long 2.5-4 millimeter.The long 4-5 centimetre of aggregate fruit, the long 2-3 centimetre of carpopodium; Follicle radish is only several growth sometimes, oval or the shape of falling ovum oval shape, long 8-12 millimeter, the short beak of top tool.The wide ellipse of seed, is about 8 millimeters.The florescence 3-5 month, the fruit phase 6-8 month.For good garden is viewed and admired and Shade Trees.
Along with the effect of Michelia shiluensis in green industry becomes increasingly conspicuous, its utilization and rearing new variety are paid attention to day by day.And seminal propagation is the main method of cultivating new varieties and breeding stock.But Michelia shiluensis seed coat is hard, have confinement effect to the absorption of moisture and respiratory metabolism, and pericarp, seed coat, in embryo all containing abscisic acid (ABA), have the characteristic of deep-sleep, therefore germination rate is general very low and irregular.For improving breeding efficiency and resource utilization, need to process seed, conventional method for treating seeds safety is lower, and treatment effect is undesirable.
The present invention is by Seed Germination Test, and the method adopting physics and chemistry process to combine, solves the problem that Michelia shiluensis seed germination rate is low.
Summary of the invention
The present invention is directed to some problems in currently available technology, provide a kind of method promoting Michelia shiluensis seed germination.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is: provide a kind of method promoting Michelia shiluensis seed germination, comprise the steps:
1), Stratificated treatment: Michelia shiluensis seed is husky with wet, perlite mixes, and wherein wet husky water content is 40%, after plastic bag sealing, is placed in 3 DEG C of refrigerator low temperature treatment, takes out and clean after 20d;
2), dense sulfuric acid treatment: by through step 1) seed after process is soaked in the concentrated sulfuric acid, process 3min, takes out and cleans;
3), sodium bicarbonate process: by through step 2) seed after process is soaked in the sodium bicarbonate solution of concentration 2% of 65 DEG C, constantly stir, process 20min, takes out and cleans;
4), the process of plant catabolic enzyme: by through step 3) seed after process is immersed in the plant catabolic enzyme solution of concentration 4%, 20 DEG C by day, night processes 40h under the room temperature condition of 10 DEG C, take out and clean;
5), 6-BA process: by through step 4) seed after process is soaked in the 6-BA solution of 120mg/L, 25 DEG C by day, night processes 24h under the room temperature condition of 13 DEG C, take out and clean;
6), calcium chloride process: by through step 5) seed after process is soaked in the calcium chloride solution of 25mmol/L, 20 DEG C by day, night processes 12h under the room temperature condition of 15 DEG C, take out and clean;
7), plant extraction liquid process: by through step 6) seed after process is soaked in plant extraction liquid, 27 DEG C by day, night processes 36h under the room temperature condition of 14 DEG C, take out and clean.
In described step 1, Michelia shiluensis seed and wet sand, perlite mixed volume are than being 1:3:2.
The preparation method of described plant extraction liquid is: (1) is dry by axletree blade of grass, pulverizing obtains powder I, ethanol and powder I in mass ratio 6:1 ~ 8:1 mix, refluxing extraction 10 ~ 30 minutes, filter extract, filtrate is concentrated into dry, obtains solid content II;
(2) solid content II step (1) obtained and water in mass ratio 1:200 ~ 1:500 mix, and ultrasonic process 2 ~ 4 minutes, obtains solid content II suspension and plant extraction liquid.
Calcium ion is the necessary mineral nutrient element of plant growth, is also a kind of important Metabolism regulation material.The interpolation of External Calcium can improve the content of soluble sugar in endosperm, can also improve the activity of amylase in Seed Germination, lipase, glutamic-pyruvic transaminase, and then promotes the sprouting of seed.
Plant catabolic enzyme is a kind of protein mixture with the decomposition function of organization such as plant cell wall, cell membrane, mainly comprises cellulolytic enzyme, pectin hydrolase etc.There is the performance of general protein, also there is the specificity as a kind of enzyme simultaneously.The inventive method can make Michelia shiluensis percentage of seedgermination improve 60%, can significantly improve germination regularity simultaneously, have broad application prospects, for the breeding of the cultivation and stock that promote Michelia shiluensis new varieties has great importance.
Embodiment
Below preferred embodiment of the present invention is described in detail, can be easier to make advantages and features of the invention be readily appreciated by one skilled in the art, thus more explicit defining is made to protection scope of the present invention.
The embodiment of the present invention comprises:
Embodiment 1
Promote a method for Michelia shiluensis seed germination, comprise the steps:
1), Stratificated treatment: Michelia shiluensis seed is husky with wet, perlite mixes, and wherein wet husky water content is 40%, after plastic bag sealing, is placed in 3 DEG C of refrigerator low temperature treatment, takes out and clean after 20d;
2), dense sulfuric acid treatment: by through step 1) seed after process is soaked in the concentrated sulfuric acid, process 3min, takes out and cleans;
3), sodium bicarbonate process: by through step 2) seed after process is soaked in the sodium bicarbonate solution of concentration 2% of 65 DEG C, constantly stir, process 20min, takes out and cleans;
4), the process of plant catabolic enzyme: by through step 3) seed after process is immersed in the plant catabolic enzyme solution of concentration 4%, 20 DEG C by day, night processes 40h under the room temperature condition of 10 DEG C, take out and clean;
5), 6-BA process: by through step 4) seed after process is soaked in the 6-BA solution of 120mg/L, 25 DEG C by day, night processes 24h under the room temperature condition of 13 DEG C, take out and clean;
6), calcium chloride process: by through step 5) seed after process is soaked in the calcium chloride solution of 25mmol/L, 20 DEG C by day, night processes 12h under the room temperature condition of 15 DEG C, take out and clean;
7), plant extraction liquid process: by through step 6) seed after process is soaked in plant extraction liquid, 27 DEG C by day, night processes 36h under the room temperature condition of 14 DEG C, take out and clean.
In described step 1, Michelia shiluensis seed and wet sand, perlite mixed volume are than being 1:3:2.
The preparation method of described plant extraction liquid is: (1) by dry for axletree blade of grass, pulverize and obtain powder I, ethanol and powder I in mass ratio 6:1 mix, refluxing extraction 10 minutes, filtration extract, filtrate is concentrated into dry, obtains solid content II;
(2) solid content II step (1) obtained and water in mass ratio 1:200 mix, and ultrasonic process 2 ~ 4 minutes, obtains solid content II suspension and plant extraction liquid.
Embodiment 2
Promote a method for Michelia shiluensis seed germination, comprise the steps:
1), Stratificated treatment: Michelia shiluensis seed is husky with wet, perlite mixes, and wherein wet husky water content is 40%, after plastic bag sealing, is placed in 3 DEG C of refrigerator low temperature treatment, takes out and clean after 20d;
2), dense sulfuric acid treatment: by through step 1) seed after process is soaked in the concentrated sulfuric acid, process 3min, takes out and cleans;
3), sodium bicarbonate process: by through step 2) seed after process is soaked in the sodium bicarbonate solution of concentration 2% of 65 DEG C, constantly stir, process 20min, takes out and cleans;
4), the process of plant catabolic enzyme: by through step 3) seed after process is immersed in the plant catabolic enzyme solution of concentration 4%, 20 DEG C by day, night processes 40h under the room temperature condition of 10 DEG C, take out and clean;
5), 6-BA process: by through step 4) seed after process is soaked in the 6-BA solution of 120mg/L, 25 DEG C by day, night processes 24h under the room temperature condition of 13 DEG C, take out and clean;
6), calcium chloride process: by through step 5) seed after process is soaked in the calcium chloride solution of 25mmol/L, 20 DEG C by day, night processes 12h under the room temperature condition of 15 DEG C, take out and clean;
7), plant extraction liquid process: by through step 6) seed after process is soaked in plant extraction liquid, 27 DEG C by day, night processes 36h under the room temperature condition of 14 DEG C, take out and clean.
In described step 1, Michelia shiluensis seed and wet sand, perlite mixed volume are than being 1:3:2.
The preparation method of described plant extraction liquid is: (1) by dry for axletree blade of grass, pulverize and obtain powder I, ethanol and powder I in mass ratio 7:1 mix, refluxing extraction 20 minutes, filtration extract, filtrate is concentrated into dry, obtains solid content II;
(2) solid content II step (1) obtained and water in mass ratio 1:300 mix, and ultrasonic process 2 ~ 4 minutes, obtains solid content II suspension and plant extraction liquid.
Embodiment 3
Promote a method for Michelia shiluensis seed germination, comprise the steps:
1), Stratificated treatment: Michelia shiluensis seed is husky with wet, perlite mixes, and wherein wet husky water content is 40%, after plastic bag sealing, is placed in 3 DEG C of refrigerator low temperature treatment, takes out and clean after 20d;
2), dense sulfuric acid treatment: by through step 1) seed after process is soaked in the concentrated sulfuric acid, process 3min, takes out and cleans;
3), sodium bicarbonate process: by through step 2) seed after process is soaked in the sodium bicarbonate solution of concentration 2% of 65 DEG C, constantly stir, process 20min, takes out and cleans;
4), the process of plant catabolic enzyme: by through step 3) seed after process is immersed in the plant catabolic enzyme solution of concentration 4%, 20 DEG C by day, night processes 40h under the room temperature condition of 10 DEG C, take out and clean;
5), 6-BA process: by through step 4) seed after process is soaked in the 6-BA solution of 120mg/L, 25 DEG C by day, night processes 24h under the room temperature condition of 13 DEG C, take out and clean;
6), calcium chloride process: by through step 5) seed after process is soaked in the calcium chloride solution of 25mmol/L, 20 DEG C by day, night processes 12h under the room temperature condition of 15 DEG C, take out and clean;
7), plant extraction liquid process: by through step 6) seed after process is soaked in plant extraction liquid, 27 DEG C by day, night processes 36h under the room temperature condition of 14 DEG C, take out and clean.
In described step 1, Michelia shiluensis seed and wet sand, perlite mixed volume are than being 1:3:2.
The preparation method of described plant extraction liquid is: (1) by dry for axletree blade of grass, pulverize and obtain powder I, ethanol and powder I in mass ratio 8:1 mix, refluxing extraction 30 minutes, filtration extract, filtrate is concentrated into dry, obtains solid content II;
(2) solid content II step (1) obtained and water in mass ratio 1:500 mix, and ultrasonic process 2 ~ 4 minutes, obtains solid content II suspension and plant extraction liquid.
The seed germination rate of embodiment 1-3 reaches 78%, 82%, 80% respectively, relative to only carry out Stratificated treatment contrast seed 20%, improve about 60%, relative to only carried out step 1-6 contrast seed 40%, improve about 40%.
The foregoing is only embodiments of the invention; not thereby the scope of the claims of the present invention is limited; every utilize description of the present invention to do equivalent structure or equivalent flow process conversion; or be directly or indirectly used in other relevant technical fields, be all in like manner included in scope of patent protection of the present invention.
Claims (3)
1. promote a method for Michelia shiluensis seed germination, it is characterized in that: comprise the steps:
1), Stratificated treatment: Michelia shiluensis seed is husky with wet, perlite mixes, and wherein wet husky water content is 40%, after plastic bag sealing, is placed in 3 DEG C of refrigerator low temperature treatment, takes out and clean after 20d;
2), dense sulfuric acid treatment: by through step 1) seed after process is soaked in the concentrated sulfuric acid, process 3min, takes out and cleans;
3), sodium bicarbonate process: by through step 2) seed after process is soaked in the sodium bicarbonate solution of concentration 2% of 65 DEG C, constantly stir, process 20min, takes out and cleans;
4), the process of plant catabolic enzyme: by through step 3) seed after process is immersed in the plant catabolic enzyme solution of concentration 4%, 20 DEG C by day, night processes 40h under the room temperature condition of 10 DEG C, take out and clean;
5), 6-BA process: by through step 4) seed after process is soaked in the 6-BA solution of 120mg/L, 25 DEG C by day, night processes 24h under the room temperature condition of 13 DEG C, take out and clean;
6), calcium chloride process: by through step 5) seed after process is soaked in the calcium chloride solution of 25mmol/L, 20 DEG C by day, night processes 12h under the room temperature condition of 15 DEG C, take out and clean;
7), plant extraction liquid process: by through step 6) seed after process is soaked in plant extraction liquid, 27 DEG C by day, night processes 36h under the room temperature condition of 14 DEG C, take out and clean.
2. the method for claim 1, is characterized in that: in described step 1, Michelia shiluensis seed and wet sand, perlite mixed volume are than being 1:3:2.
3. the method for claim 1, it is characterized in that: the preparation method of described plant extraction liquid is: (1) is dry by axletree blade of grass, pulverizing obtains powder I, ethanol and powder I in mass ratio 6:1 ~ 8:1 mix, refluxing extraction 10 ~ 30 minutes, filter extract, filtrate is concentrated into dry, obtains solid content II;
(2) solid content II step (1) obtained and water in mass ratio 1:200 ~ 1:500 mix, and ultrasonic process 2 ~ 4 minutes, obtains solid content II suspension and plant extraction liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510183070.XA CN104813773A (en) | 2015-04-17 | 2015-04-17 | Method for promoting michelia shiluensis seed germination |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510183070.XA CN104813773A (en) | 2015-04-17 | 2015-04-17 | Method for promoting michelia shiluensis seed germination |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104813773A true CN104813773A (en) | 2015-08-05 |
Family
ID=53724485
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510183070.XA Pending CN104813773A (en) | 2015-04-17 | 2015-04-17 | Method for promoting michelia shiluensis seed germination |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104813773A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106612732A (en) * | 2016-12-13 | 2017-05-10 | 韦克康 | Method for raising seedlings from radix bupleuri seeds |
| CN106717270A (en) * | 2016-12-13 | 2017-05-31 | 韦克康 | Paniculate swallowwort seed seedling-raising method |
| CN106717269A (en) * | 2016-12-13 | 2017-05-31 | 韦克康 | Seed of Platycodon Grandiflorum method for culturing seedlings |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103430657A (en) * | 2013-08-16 | 2013-12-11 | 苏州仁成生物科技有限公司 | Method for promoting seed germination of cercidiphyllum japonicum and improving germination ratio of seeds |
-
2015
- 2015-04-17 CN CN201510183070.XA patent/CN104813773A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103430657A (en) * | 2013-08-16 | 2013-12-11 | 苏州仁成生物科技有限公司 | Method for promoting seed germination of cercidiphyllum japonicum and improving germination ratio of seeds |
Non-Patent Citations (1)
| Title |
|---|
| 马小英等: "木兰科植物种子繁殖研究概况", 《种子》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106612732A (en) * | 2016-12-13 | 2017-05-10 | 韦克康 | Method for raising seedlings from radix bupleuri seeds |
| CN106717270A (en) * | 2016-12-13 | 2017-05-31 | 韦克康 | Paniculate swallowwort seed seedling-raising method |
| CN106717269A (en) * | 2016-12-13 | 2017-05-31 | 韦克康 | Seed of Platycodon Grandiflorum method for culturing seedlings |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104350912B (en) | The cuttage breeding method of south blue berry | |
| CN104186303B (en) | Dragon fruit pollen suspension and preparation method thereof, pollinating method | |
| CN102668853B (en) | Blueberry twig plant cuttage root-taking cultivation method | |
| CN104813821B (en) | Cultivation method for prompting blossoming and fruiting of tomato | |
| CN106007887A (en) | Organic liquid suspension zinc fertilizer and preparation method thereof | |
| CN104813773A (en) | Method for promoting michelia shiluensis seed germination | |
| CN104604459A (en) | Hard branch cutting propagation method of styrax japonicus seedlings | |
| CN102558445A (en) | Preparation of agricultural water-retaining agent | |
| CN104663175A (en) | Cultivation method for iodine-containing spinach with high survival rate | |
| CN106187499A (en) | A kind of nursery stock fertilizer with bacteriostasis | |
| CN105284239A (en) | Method for promoting germination and sowing of Chinese scholar tree seeds | |
| CN102311290B (en) | Drought-resisting agent for sugarcane and preparation method thereof | |
| CN105191723A (en) | Camellia oleifera planting method | |
| CN104813771A (en) | Method for promoting michelia sphaerantha seed germination | |
| CN105266164A (en) | Manufacturing method of selenium-enriched functional water with high energy quantum | |
| CN103563619B (en) | Ternstroemia gymnanthera intercropping cuttage breeding method | |
| CN104813770A (en) | Method for promoting horseshoe geranium seed germination | |
| CN106242738A (en) | A kind of selenium-enriched foliage fertilizer material | |
| CN105367224A (en) | Nutrient solution for improving organism-caused disease resistance of oil peony | |
| CN108530158A (en) | Fertilizer for the wooden indoor culture in thousand | |
| CN105399492A (en) | Nutrient solution for improving photosynthesis of oil peony | |
| CN105123017A (en) | Method for promoting canna seed germination | |
| CN114642129B (en) | Callicarpa nudiflora cutting planting method | |
| CN108440088A (en) | Water lily nutritious fertilizer | |
| CN108383603A (en) | A kind of paper mulberry water-holding slow-release growth promotion fertilizer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150805 |