CN110651558A - Method for improving germination rate of ramie seeds - Google Patents

Abstract

The invention relates to the technical field of seed germination acceleration, and discloses a method for improving the germination rate of ramie seeds, which comprises the steps of seed treatment and seed germination acceleration, wherein selected ramie seeds are disinfected, the disinfected seeds are uniformly laid in a culture dish padded with two layers of filter paper, a abscisic acid solution is poured into the culture dish to immerse the seeds for 24 hours, then the seeds after the treatment are placed in a climatic chamber for 6-10 days to be subjected to laminated germination acceleration treatment, and the temperature of the climatic chamber is 18-25 ℃. The method is simple, low in cost, simple and convenient in management process, easy to master and operate, high in seed germination rate, short in germination time and neat in germination due to the adoption of constant-temperature germination acceleration.

Description

Method for improving germination rate of ramie seeds

Technical Field

The invention relates to the technical field of seed germination acceleration, in particular to a method for improving the germination rate of ramie seeds.

Background

The ramie seeds are very small, and although each kilogram of ramie has about 1500-3400 ten thousand seeds, the emergence rate of the ramie seeds is less than 10 percent, and the ramie seeds have the defect of irregular emergence. The factors influencing the germination rate of the seeds are many, and comprise various influencing factors such as moisture, temperature, oxygen, bacteria, management and the like.

At present, the ramie seeds have low germination rate, slow and irregular germination, and waste of the seeds is caused. Wushihui et al showed that endogenous abscisic acid (ABA) plays an important role as a regulator during seed embryo development. Abscisic acid can make embryo normally mature and inhibit premature germination, and in the dormant late stage abscisic acid has the function of promoting seed after-ripening and can promote the formation of seed embryo so as to achieve the purpose of early germination.

Disclosure of Invention

Based on the problems, the invention provides a method for improving the germination rate of ramie seeds, which can accelerate the seeds to germinate in advance at constant temperature, improve the germination rate, shorten the germination time and improve the emergence regularity.

In order to solve the technical problems, the invention provides the following technical scheme:

a method for improving the germination rate of ramie seeds comprises the following steps:

s1: seed treatment

Sterilizing selected ramie seeds, uniformly spreading the sterilized seeds in a culture dish padded with two layers of filter paper, pouring a abscisic acid solution into the culture dish to immerse the seeds, and soaking for 24 hours;

s2: seed germination acceleration

And (4) putting the seeds treated in the step (S1) into an artificial climate box for 6-10 days for layering and germination accelerating treatment, wherein the temperature of the artificial climate box is 18-25 ℃.

Further, the concentration of abscisic acid in step S1 was 20 mg/L.

Further, the temperature of the climatic chamber in step S2 was 25 ℃.

Compared with the prior art, the invention has the beneficial effects that: the method is simple, low in cost, simple and convenient in management process, easy to master and operate, high in seed germination rate, short in germination time and neat in germination due to the adoption of constant-temperature germination acceleration.

Drawings

FIG. 1 is a diagram showing the germination of the ramie seeds of this example after abscisic acid treatment.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to examples and accompanying drawings, and the exemplary embodiments and descriptions thereof are only used for explaining the present invention and are not meant to limit the present invention.

Example (b):

a method for improving the germination rate of ramie seeds comprises the following steps:

s1: seed treatment

Sterilizing selected ramie seeds, uniformly spreading the sterilized seeds in a culture dish on which two layers of filter paper are placed, pouring abscisic acid solution into the culture dish to immerse the seeds, wherein the use concentration of abscisic acid is 20mg/L, and soaking for 24 hours;

s2: seed germination acceleration

The seeds treated in step S1 are placed in a climatic chamber for 6-10 days for stratification, the temperature of the climatic chamber is 18-25 ℃, and the temperature of the climatic chamber in this embodiment is 25 ℃.

In order to verify the practicability and effect of the invention, the following comparative experiments were carried out using the method: in comparative experiments, two hormones, indoleacetic acid (IAA) and abscisic acid (ABA), were selected, both set at the following concentrations: 10mg/L, 20mg/L and 40mg/L, the experiment is provided with two temperature parameters of 18 ℃ and 25 ℃ and is provided with the following 12 experimental groups:

the related parameters in the above experimental groups were applied to the germination accelerating treatments in steps S1 and S2 of this example, and the components and parameters related to the seed germination of the treated ramie seeds were measured, and the measurement results are shown in tables 1 and 2:

TABLE 1 Effect of IAA and ABA at different temperatures on the germination-related components of Ramie seeds

TABLE 2 influence of IAA and ABA at different temperatures on parameters relevant to ramie seed germination

From tables 1 and 2, it can be seen that different hormone treatments at 18 ℃ all showed promoting effects on ramie seed germination, wherein ABA (abscisic acid) is more capable of promoting ramie seed germination compared with IAA (indoleacetic acid) and is more remarkable at a hormone concentration of 20 mg/L; the promoting effect of the hormone on the ramie seed germination at 25 ℃ is more obvious in 6-10 days, the promoting effect of ABA (abscisic acid) is more obvious, and the germination rate reaches the maximum at 20mg/L and is obviously higher than that of other treatment groups.

FIG. 1 is a germination diagram of the ramie seeds of this example after abscisic acid treatment, and it can be seen from FIG. 1 that the ramie seeds germinate well.

The above is an embodiment of the present invention. The embodiments and specific parameters in the embodiments are only for the purpose of clearly illustrating the verification process of the invention and are not intended to limit the scope of the invention, which is defined by the claims, and all equivalent structural changes made by using the contents of the specification and the drawings of the present invention should be covered by the scope of the present invention.

Claims (3)

1. A method for improving the germination rate of ramie seeds is characterized by comprising the following steps:

s1: seed treatment

Sterilizing selected ramie seeds, uniformly spreading the sterilized seeds in a culture dish padded with two layers of filter paper, pouring a abscisic acid solution into the culture dish to immerse the seeds, and soaking for 24 hours;

s2: seed germination acceleration

And (4) putting the seeds treated in the step (S1) into an artificial climate box for 6-10 days for layering and germination accelerating treatment, wherein the temperature of the artificial climate box is 18-25 ℃.

2. The method of claim 1, wherein the abscisic acid concentration in step S1 is 20 mg/L.

3. The method of claim 1, wherein the climatic chamber of step S2 is at a temperature of 25 ℃.

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