CN105284404A - Method for promoting growth of Poacynum pictum by inoculating arbuscular mycorrhizal fungi - Google Patents
Method for promoting growth of Poacynum pictum by inoculating arbuscular mycorrhizal fungi Download PDFInfo
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- CN105284404A CN105284404A CN201510850010.9A CN201510850010A CN105284404A CN 105284404 A CN105284404 A CN 105284404A CN 201510850010 A CN201510850010 A CN 201510850010A CN 105284404 A CN105284404 A CN 105284404A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/10—Mycorrhiza; Mycorrhizal associations
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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Abstract
The invention relates to a method for promoting growth of Poacynum pictum by inoculating arbuscular mycorrhizal fungi. The method comprises the following steps: collecting and processing seeds; sprouting and inoculating; observing the growth situation; measuring infection. An experimental result proves that the four important growth indexes, namely, average plant height, average growth rate, cluster width and leaf number of the Poacynum pictum plant, after the Glomus mosseae is inoculated with Poacynum pictum, are obviously higher than the four important growth indexes of two non-inoculating contrast groups and other AM fungi treating groups. Meanwhile, the infection rate and infection strength of the Glomus mosseae to the Poacynum pictum are higher than the infection rate and infection strength to all the other treating groups, so that the growth for the strain after the Glomus mosseae is inoculated with Poacynum pictum is obviously boosted.
Description
Technical field
The present invention relates to a kind of Arbuscular Mycorrhizal Fungi and promote the method that hemp (Poacynumpictum) grows.
Background technology
Mycorhiza (Mycorrhizae) is a kind of general plant symbiosis phenomenon of natural world, arbuscular mycorrhiza (AM fungi) is then the widest, the closest with the Agriculture, forestry And Animal Husbandry relation class mycorhiza that distributes, and on the earth, the vascular plant of more than 90% can form arbuscular mycorrhiza.Very close in the relation of the reconstruction of arid area vegetation and arbuscular mycorrhiza (AM fungi), some plants must rely on mycorhiza community to survive or to complete its life cycle; The number that plant roots and fungi community make a profit and the scope of time and arbuscular mycorrhiza (AM fungi) group relevant, the quick growth of plant phase is relevant with the high level of fungal colonization in general; Arbuscular mycorrhiza (AM fungi) plays important role in Arid land ecosystems, directional inoculation arbuscular mycorrhiza (AM fungi) in plant introduction sexual reproduction process, to help that advantitious plant survives, grows, population child care, and theoretical foundation can be provided for the recovery of ecosystem function, medicinal to arid biogeographic zone Precious, Rare, Endangered, economic plants to introduce a fine variety breeding meaning great.
Have large-area arid area in China western part, cause land capability to decline because the unreasonable activity in production of the mankind and fragile ecological environment interact, land resources is lost, and the threat of desertification, desertization is day by day serious.Some number of plants in the ecosystem with important function reduce or are on the verge of impasse.
Research show the reconstruction of arid area vegetation and the relation of arbuscular mycorrhiza (AM fungi) very close.Some plants must rely on mycorhiza community to survive or to complete its life cycle; Other plants have benefited from mycorhiza under certain condition, are facultative communities; And have some plants being infected rear output and survival rate decline by arbuscular mycorrhiza (AM fungi).The number that plant roots and fungi community make a profit and the scope of time and arbuscular mycorrhiza (AM fungi) group relevant, the evidence of the functional contact that may exist between advantitious plant and arbuscular mycorrhiza (AM fungi), the pattern likely by detecting itself and root arbuscular mycorrhiza (AM fungi) group finds; The ecological characteristic of habitat characteristics (soil physical chemistry the hydrology weather etc.) or arbuscular mycorrhiza (AM fungi) may determine the dynamic change of arbuscular mycorrhiza (AM fungi).And the difference of plant growth ground characteristic sum plant site may cause arbuscular mycorrhiza (AM fungi) difference that taxon is very large, thus growing of plant can be affected, because different arbuscular mycorrhizas (AM fungi) taxon also has very large difference in the ability of nutrient and Promoting plant growth that they are supplied to plant, especially very strong to mycorrhizal dependency plant. the quick growth of plant phase is relevant with the high level of fungal colonization in general, also relevant with the kind of fungi.Research finds, arbuscular mycorrhiza (AM fungi) has the ability to be come by the distribution of nutrition between aulophyte the symbiotic relation of regulating plant, and the nutritional amt that a Plants obtains determines the symbiosis ability of its competitive ability and it and other plant.Arbuscular mycorrhiza (AM fungi) abundant species and group's composition have the words of similitude at a Plants of different niches, so the structure of plant community may be subject to the impact of arbuscular mycorrhiza (AM fungi) kind, conversely, aulophyte also may can affect the structure of arbuscular mycorrhiza (AM fungi) group, but also likely plant and arbuscular mycorrhiza (AM fungi) kind show that a kind of interdepending adapts to the relation of these habitat soil and weather conditions.
The effect that aulophyte produces may change along with arbuscular mycorrhiza (AM fungi) kind composition, arbuscular mycorrhiza (AM fungi) effect to plant is also incoordinate, arbuscular mycorrhiza (AM fungi) group changing or locally change an ecosystem will have influence on ensuing process, reduce because of agriculture behavior in semiarid Mediterranean Region arbuscular mycorrhiza (AM fungi) population quantity, until agro-farming just recovers after stopping 45 years, Stribley (1987) advises that mycorhiza is very important with survival for the establiss of plant in natural ecosystems, and emphasize that the indigenous bacterium kinds of root in mensuration ecosystem is also extremely important.
Therefore, in Arid land ecosystems, arbuscular mycorrhiza (AM fungi) plays important role, and it not only likely decides the kind of plant, the distribution of nutritional resource with other biology together with abiotic factor, even likely affects structure of plant community and diversity.In the process of introducing a fine variety, kind, quantity and the ecological characteristic thereof of the symbiosis arbuscular mycorrhiza (AM fungi) with plant are familiar with to some extent, so not only will help to introduce a fine variety and transplant plant survival, growth, population child care, and theoretical foundation can be provided for the recovery of ecosystem function, to implement more effective recovery measure; In the production of arid area agriculture forest and husbandry, medicinal plant exploitation, plant introduces a fine variety artificial Arbuscular Mycorrhizal Fungi (AM fungi) in process, by promoting the growing of plant, strengthening plant resistance to environment stress, improving crop yield and quality, improve medicament effective component.
Summary of the invention
The object of the invention is, a kind of Arbuscular Mycorrhizal Fungi is provided to promote the method for hemp growth, the method is by seed collection process, sprout and inoculation, growing state is observed and is infected determination step and completes, show through experimental result: E Glomus mosseae Glomusmosseae inoculates hemp Poacynumpictum, to the average plant height of hemp Poacynumpictum plant, average growth rate, clump width, the number of blade four important growth indexes all present apparently higher than to two non-seeded control groups and other AM fungal biodegradation groups.E Glomus mosseae Glomusmosseae has high infection rate and high infection strength to hemp Poacynumpictum simultaneously, higher than other all processed group, therefore there is obvious facilitation to the growth after hemp Poacynumpictum inoculation E Glomus mosseae Glomusmosseae bacterial classification.
Arbuscular Mycorrhizal Fungi of the present invention promotes the method for hemp growth, and the method follows these steps to carry out:
Seed collection process:
A, pick up from the hemp seed of full health, after natural seasoning, soak 15min with NaClO solution, sterile water washes down, and after seed soaking 24h, puts into the sterilized culture dish that double-layer filter paper is housed, processes;
Sprout and inoculation:
B, the hemp seed after step a sterilizing inserted climatic cabinate and cultivate, climate box setting day temperature every day 25 DEG C, cultivates 14 hours, night temperatures 14 DEG C, cultivates 10 hours, after hemp seed germination, grow to 1-2cm for subsequent use;
C, select the nutritive cube 6 groups of experimental field 10cm × 15cm in the wild, often organize 15 nutritive cubes, then by the sandy soil after conventional method sterilizing thick for each nutritive cube paving 10cm in 6 groups of nutritive cubes, at sandy soil surface even spreading 6 kinds of A Glomus etunicatums respectively, the ivory white sacculus of B is mould, C clumping sacculus is mould, the nearly bright sacculus of D is mould, E Glomus mosseae, washed-out sand microbial inoculum containing fungal spore diameter 2mm during F stratiform sacculus is mould, spreading thickness 2-3mm, then respectively 6 kinds of microbial inoculums surface paving 2mm thick through conventional method sterilizing sandy soil, be uniformly dispersed on sandy soil again and place seedling 5 strain of step b hemp sprouting for subsequent use, 2mm that seedling is tiled again thick through conventional method sterilizing sandy soil, then water,
Growing state is observed and is infected mensuration:
Start the plant height to each plant after d, 10d of emerging, clump width, Cong Jing, the number of blade, growth rate data carry out periodic observation, get root segment after 90d and fix, more than 100 sections are got in each process, and about more than 800 root segments, infect observation experiment for next step altogether.
Spore density in fungal spore in step c is 25-100 spore/ml.
Hemp Poacynumpictum (Schrenk) Baill. Apocynaceae, hemp belongs to, and protection species in I grade, Xinjiang are arid biogeographic zone rare or endangered species species.
In the present invention, arid biogeographic zone rare or endangered species hemp Poacynumpictum seed gathers by Chinese Academy of Sciences's Turpan Desert Botanical Garden or at it; Arbuscular mycorrhizal fungi 6 is A Glomus etunicatum Glomusetunicatum, the mould Glomuseburneum of the ivory white sacculus of B, the mould Glomusaggregatum of C clumping sacculus, the mould Glomusclaroideum of the nearly bright sacculus of D, E Glomus mosseae Glomusmosseae, the mould Glomuslamellosum of F stratiform sacculus expands numerous by Inst. of Plant Nutrition &. Resource, Beijing City Academy of Agricultural &. Fo's " arbuscular mycorrhizal fungi germplasm resource bank (BankofGlomeromycotainChina, BGC) " and provides.
Accompanying drawing explanation
Fig. 1 is that the present invention grows schematic diagram.
Embodiment
As subjects hemp Poacynumpictum seed, between 2013-2014 6-8 month fructescence, collect the seed of full health respectively, pick up from Chinese Academy of Sciences's Turpan Desert Botanical Garden.
Embodiment
Seed collection process:
A, pick up from the hemp Poacynumpictum seed of full health, after natural seasoning, soak 15min with NaClO solution, sterile water washes down, and after seed soaking 24h, puts into the sterilized culture dish that double-layer filter paper is housed, processes;
Sprout and inoculation:
B, the hemp Poacynumpictum seed after step a sterilizing inserted climatic cabinate and cultivate, temperature 25 DEG C, cultivates 14 hours, temperature 14 DEG C, cultivates 10 hours, after hemp Poacynumpictum seed germination, grows to 1-2cm for subsequent use;
C, select the nutritive cube 6 groups of experimental field 10cm × 15cm in the wild, often organize 15 nutritive cubes, then by the sandy soil (soil data is in table 1 table 2) after conventional method sterilizing thick for each nutritive cube paving 10cm in 6 groups of nutritive cubes, washed-out sand microbial inoculum containing fungal spore diameter 2mm in sandy soil surface respectively even spreading 6 kinds of A Glomus etunicatum Glomusetunicatum, washed-out sand microbial inoculum containing fungal spore diameter 2mm in the mould Glomuseburneum of the ivory white sacculus of B, washed-out sand microbial inoculum containing fungal spore diameter 2mm in the mould Glomusaggregatum of C clumping sacculus, washed-out sand microbial inoculum containing fungal spore diameter 2mm in the mould Glomusclaroideum of the nearly bright sacculus of D, washed-out sand microbial inoculum containing fungal spore diameter 2mm in E Glomus mosseae Glomusmosseae, washed-out sand microbial inoculum containing fungal spore diameter 2mm in the mould Glomuslamellosum of F stratiform sacculus, spreading thickness 2-3mm, again respectively 6 kinds of microbial inoculums surface paving 2mm thick through conventional method sterilizing sandy soil, then be uniformly dispersed on sandy soil and place seedling 5 strain of step b hemp Poacynumpictum sprouting for subsequent use, 2mm that seedling is tiled again thick through conventional method sterilizing sandy soil, then water, be provided with two contrasts simultaneously, blank CK1 and quartz sand contrast CK2, the aseptic sandy soil of preset 10cm directly plant 5 strain hemp seedling to blank CK1, the sterilizing sandy soil that 2mm that seedling is tiled again is thick, repeat for 15 times, quartz sand contrast contrast CK2 is that the aseptic sandy soil of preset 10cm first tile 3mm physico chemical property wash sand with identical aseptic of the Inoculant of spore without spore, repave sandy soil after the thick sterilizing of 2mm, be uniformly dispersed and put to 5 strain hemp seedling, the sterilizing sandy soil that 2mm that seedling is tiled again is thick, repeat for 15 times,
D, growing state are observed and are infected mensuration
Emerge to start plant height to each plant after 10d, clump width, Cong Jing, the number of blade, growth rate data carry out periodic observation, get root segment after 90d and fix, 80-100 section is got in often kind of each process, and about more than 800 root segments, infect observation experiment for next step altogether.
Soil nutrient data: table 1
| Sample ID | Organic carbon (g/kg) | Full nitrogen (g/kg) | Full phosphorus (g/kg) | Speed nitrogen (mg/kg) | Speed phosphorus (mg/kg) |
| 14.165 | 0.167 | 1.162 | 562.464 | 5.976 |
Soil nutrient data: table 2
| Sample ID | PH | Cl -(mg/g) | SO 4 2-(mg/g) | Ca 2+(mg/g) | K +(mg/g) | Mg 2+(mg/g) | Na +(mg/g) | CO 3 2-(mg/g) |
| 7.92 | 0.15211 | 1.275935 | 0.399442 | 0.046193 | 0.05188 | 0.28248 | 0 |
Hemp (Poacynumpictum) upgrowth situation under the different AM fungal biodegradation of table 3
Under the different AM fungal biodegradation of table 4, hemp (Poacynumpictum) infects data
According to the observed result display of 151d in vegetative period, after E Glomus mosseae Glomusmosseae process, average plant height 35.50 ± 0.50 and the average growth rate 0.24 ± 0.003 of plant presents the phenomenon apparently higher than control group and other AM fungal biodegradation groups, clump width, the number of blade is also higher than to two non-seeded control groups and other AM fungal biodegradation groups, and there are high infection rate 93.33 and high infection strength 47.33, show that the growth of E Glomus mosseae Glomusmosseae to hemp Poacynumpictum creates obvious facilitation.In contrast, compared with control group and other AM fungal biodegradation groups, the average plant height 18.78 ± 5.29 of F stratiform sacculus mould Glomuslamellosum processed group is lower slightly with average growth rate 0.12 ± 0.04.All the other 4 A Glomus etunicatum Glomusetunicatum, the mould Glomusclaroideum of the nearly bright sacculus of the ivory white sacculus of B mould Glomuseburneum, C clumping sacculus mould Glomusaggregatum, D is not relative to control group making significant difference to plant growth condition.Therefore, the hemp Poacynumpictum upgrowth situation after E Glomus mosseae Glomusmosseae fungal biodegradation has and significantly improves.
Claims (2)
1. Arbuscular Mycorrhizal Fungi promotes a method for hemp growth, it is characterized in that the method follows these steps to carry out:
Seed collection process:
A, pick up from the hemp seed of full health, after natural seasoning, soak 15min with NaClO solution, sterile water washes down, and after seed soaking 24h, puts into the sterilized culture dish that double-layer filter paper is housed, processes;
Sprout and inoculation:
B, the hemp seed after step a sterilizing inserted climatic cabinate and cultivate, climate box setting day temperature every day 25 DEG C, cultivates 14 hours, night temperatures 14 DEG C, cultivates 10 hours, after hemp seed germination, grow to 1-2cm for subsequent use;
C, select the nutritive cube 6 groups of experimental field 10cm × 15cm in the wild, often organize 15 nutritive cubes, then by the sandy soil after conventional method sterilizing thick for each nutritive cube paving 10cm in 6 groups of nutritive cubes, at sandy soil surface even spreading 6 kinds of A Glomus etunicatums respectively, the ivory white sacculus of B is mould, C clumping sacculus is mould, the nearly bright sacculus of D is mould, E Glomus mosseae, washed-out sand microbial inoculum containing fungal spore diameter 2mm during F stratiform sacculus is mould, spreading thickness 2-3mm, then respectively 6 kinds of microbial inoculums surface paving 2mm thick through conventional method sterilizing sandy soil, be uniformly dispersed on sandy soil again and place seedling 5 strain of step b hemp sprouting for subsequent use, 2mm that seedling is tiled again thick through conventional method sterilizing sandy soil, then water,
Growing state is observed and is infected mensuration:
Start the plant height to each plant after d, 10d of emerging, clump width, Cong Jing, the number of blade, growth rate data carry out periodic observation, get root segment after 90d and fix, more than 100 sections are got in each process, and about more than 800 root segments, infect observation experiment for next step altogether.
2. want the Arbuscular Mycorrhizal Fungi described in 1 to promote the method for hemp growth according to right, it is characterized in that the spore density in the fungal spore in step c is 25-100 spore/ml.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110651558A (en) * | 2019-10-12 | 2020-01-07 | 咸宁市农业科学院 | Method for improving germination rate of ramie seeds |
| CN110862929A (en) * | 2019-12-09 | 2020-03-06 | 江西农业大学 | Gloeostereum incarnatum, microbial inoculum comprising same and application thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110651558A (en) * | 2019-10-12 | 2020-01-07 | 咸宁市农业科学院 | Method for improving germination rate of ramie seeds |
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| CN110862929B (en) * | 2019-12-09 | 2020-11-06 | 江西农业大学 | Gloeostereum incarnatum, microbial inoculum comprising same and application thereof |
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