CN107926868A - A kind of method that Encarsia formosa is largely bred for host plant based on yacon - Google Patents

A kind of method that Encarsia formosa is largely bred for host plant based on yacon Download PDF

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Publication number
CN107926868A
CN107926868A CN201711313801.3A CN201711313801A CN107926868A CN 107926868 A CN107926868 A CN 107926868A CN 201711313801 A CN201711313801 A CN 201711313801A CN 107926868 A CN107926868 A CN 107926868A
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China
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plant
yacon
encarsia formosa
trialeurodes vaporariorum
largely
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CN107926868B (en
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臧连生
赵悦
戴鹏
李天昊
阮长春
张俊杰
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Jilin Agricultural University
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Jilin Agricultural University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates

Abstract

The invention discloses a kind of method for largely breeding Encarsia formosa based on yacon for host plant, is related to a kind of new method for breeding Encarsia formosa, using yacon as vector plant, using Trialeurodes vaporariorum Westwood as host, the batch production for carrying out Encarsia formosa is largely bred:(1)The cultivation of healthy yacon plant;(2)Trialeurodes vaporariorum Westwood kind worm breeds;(3)Trialeurodes vaporariorum Westwood host nymph largely breeds;(4)Encarsia formosa kind bee produces;(5)Encarsia formosa is largely bred.The present invention largely breeds Encarsia formosa by vector plant of yacon, shortens the breeding cycle of Trialeurodes vaporariorum Westwood and Encarsia formosa, further increases the quality that Encarsia formosa is largely bred, has energetically meaning to Encarsia formosa plant modification.The breeding cycle of room trialeurodes vaporariorum and Encarsia formosa, the quality that Encarsia formosa is largely bred is further increased, have energetically meaning to Encarsia formosa plant modification.

Description

A kind of method that Encarsia formosa is largely bred for host plant based on yacon
Technical field
The invention discloses a kind of method for largely breeding Encarsia formosa based on yacon for host plant, is related to a kind of numerous The new method of Encarsia formosa is educated, belongs to agriculture and forestry biological control of insect pests field.
Background technology
Aleyrodes Semiptera(Hemiptera), Aleyrodidae(Aleyrodidae), it is evil important in a kind of agricultural production One of worm.Biological control aleyrodid class pest is considered as most safely and effectively means of prevention at present.Using Encarsia formosaEncarsia formosa Gahan prevents Bemisia tabaciBemisia tabaci(Gennadius)And Trialeurodes vaporariorum WestwoodTrialeurodes vaporariorum(Westwood) it is considered as one of most classical prevention case of biological control, China Since 1978 introduce Encarsia formosa from Britain, it has been done it is a large amount of first close study and organized relevant unit carry out production and Release, release Encarsia formosa prevention Trialeurodes vaporariorum Westwood and Bemisia tabaci also achieve significant prevention effect compared with chemical prevention. But instantly the yield of Encarsia formosa still cannot, meet the wilderness demand in agricultural production.
Encarsia formosa is mainly used as vector using Trialeurodes vaporariorum Westwood and Bemisia tabaci as host, tomato and tobacco etc. at present Plant, is bred using " five Room breeding bee methods ".But current plant modification Encarsia formosa also runs into many problems, causes beautiful The breeding efficiency of aphid chalcid fly stills need to further improve.Due to Encarsia formosa breeding host and vector plant difference, The quality for breeding out Encarsia formosa is caused also to have larger difference.Therefore, the main reason for influencing Encarsia formosa, essentially consists in breeding The species and vector floristics of host, the advantage host for finding that Trialeurodes vaporariorum Westwood is Encarsia formosa is studied with reference to forefathers, And vector species is more instantly, wherein tobacco is considered as the optimal vector plant of Encarsia formosa breeding, but tobacco Encarsia formosa is bred already there are more problem, and it is to improve Encarsia formosa breeding efficiency to find a kind of new vector plant It is crucial.
Yacon category composite family(Asteraceae), Helianthus(Helianthus), dicotyledonous draft root block plant, it is planted Thing character is excellent, and plant is tall and big, and general plant height can be grown to more than 2 meters, has larger internode away from blade is to life, single-strain blade Number is maximum up to 500cm up to more than 20 pieces, single blade area2More than.Recently as yacon fruit in China's successful introduction, Cultivated area is also increasing.The simple easily management, and have a variety of modess of reproduction of yacon plantation, can utilize bulb, axillary bud And plant division is bred, single plant can produce bulb more than 10, can produce axillary bud more than 30.Compared with tobacco, yacon Pest and disease damage is less, and Trialeurodes vaporariorum Westwood is one of important pests in yacon production.Yacon plant and root tuber are main at present With food and extract sample by developmental research, but the utilization using yacon plant as host's resource insect there is no correlation to grind Study carefully report.
The content of the invention
The present invention provides a kind of method for largely breeding Encarsia formosa based on yacon for host plant, using yacon in Between host plant, using Trialeurodes vaporariorum Westwood as host, the batch production for carrying out Encarsia formosa is largely bred, it is characterised in that including snow Acquisition, the breeding of Trialeurodes vaporariorum Westwood and the breeding of Encarsia formosa of lotus fruit plant.
A kind of method for largely breeding Encarsia formosa based on yacon for host plant of the present invention, its feature exist In:Using yacon as vector plant, using Trialeurodes vaporariorum Westwood as host, the batch production for carrying out Encarsia formosa is largely bred:
(1)The cultivation of healthy yacon plant;
(2)Trialeurodes vaporariorum Westwood kind worm breeds;
(3)Trialeurodes vaporariorum Westwood host nymph largely breeds;
(4)Encarsia formosa kind bee produces;
(5)Encarsia formosa is largely bred.
The step(1)In yacon bulb nursery is carried out in culturing pot, be transplanted in container and cultivate after emergence To 8 ~ 12 blades, the every 10 d nursery of yacon seedling is a collection of, and every 10 ~ 15 d transplantings are a collection of, ensures the sufficient supply of plant.
The step(2)In Trialeurodes vaporariorum Westwood bred using the yacon plant in right 2, snow is placed in cage Lotus fruit plant, 300 ~ 500 Trialeurodes vaporariorum Westwoods of average every plant of release, when every plant of aleyrodid quantity is up to 3000 ~ 4000 adults, often 15~30 d replace 1/3 ~ 2/3 yacon plant.(Aleyrodid breeding weather conditions be 20~28 DEG C, 50~70% R.H., 14: 10 L:D).
The step(3)A large amount of breeding Trialeurodes vaporariorum Westwood host nymphs, by step(2)In be loaded with the yacon of aleyrodid kind worm Plant and step(1)In yacon plant according to 3:1 is inoculated with, i.e. every plant of yacon inoculation Trialeurodes vaporariorum Westwood adult 9000 ~ 12000, adult whitefly is removed after being inoculated with 24 h(Adult whitefly is removed using air blower, the adult whitefly of removal is numerous in worm kind Continue to breed in educating), the yacon plant after inoculation is moved into development interior and continues to develop(Trialeurodes vaporariorum Westwood develops room weather bar Part:20~28 DEG C, 50~70% R.H., 14:10 L:D), Trialeurodes vaporariorum Westwood more than 70% is developed to standby during 2~3 age on plant With.
The step(4)Middle Encarsia formosa kind bee utilizes step(3)In aleyrodid bred, breeding is placed in cage Trialeurodes vaporariorum Westwood host nymph(20~28 DEG C, 50~70% R.H. of weather conditions, 14:10 L:D), the beautiful aphid of average every plant of release Chalcid fly 500 ~ 1000, when every plant containing will sprout wings the black pupa of Encarsia formosa 40000 ~ 60000 when, every 10 ~ 20 d by 1/5 ~ 3/5 plant replaces with step(3)The plant of a large amount of breeding aleyrodids, to ensure Encarsia formosa population quantity.
The step(5)A large amount of breedings of Encarsia formosa, utilize step(4)The Encarsia formosa kind bee of breeding and step(3) The Trialeurodes vaporariorum Westwood host 1 of breeding:10 connect bee, and Encarsia formosa is removed after being inoculated with 72 h(Encarsia formosa is removed using air blower, The Encarsia formosa of removal can transfer room or be recycled to honeybee kind breeding room continue to breed), go after bee to move to development room and continue to develop (Encarsia formosa develops room weather conditions:20~28 DEG C, 50~70% R.H., 14:10 L:D), wait to be developed to black pupa, and not by Parasitic Trialeurodes vaporariorum Westwood nymph obtains black pupa after sprouting wings, and carries out black pupa collection and preservation.
The positive effect of the present invention
The positive effect of the present invention is:
Pest and disease damage occurs less in yacon cultivating process, and yacon mating system is simple;Compared with tobacco, 8 ~ 12 leaves Yacon cultivates that to go through the phase shorter, substantially reduces the vector plant cultivation time;Yacon blade area is larger, can ensure Aleyrodid and Encarsia formosa have enough oviposition spaces;In addition, the internode of yacon is conducive to Trialeurodes vaporariorum Westwood and beautiful aphid away from bigger Chalcid fly receives illumination, so as to promote the development of Encarsia formosa.
It is more suitable for the development and breeding of Trialeurodes vaporariorum Westwood, Trialeurodes vaporariorum Westwood institute on yacon with yacon compared with tobacco Development duration is needed further to shorten, egg laying amount further increases.
The Trialeurodes vaporariorum Westwood that Encarsia formosa breeds yacon has more preferable adaptability, and Encarsia formosa parasitism yacon is numerous Development duration needed for the Trialeurodes vaporariorum Westwood educated is shorter, parasitic rate and eclosion rate higher, and has preferable biological and ecological methods to prevent plant disease, pests, and erosion potential.
In general, the present invention largely breeds Encarsia formosa by vector plant of yacon, shortens greenhouse white powder Lice and the breeding cycle of Encarsia formosa, further increase the quality that Encarsia formosa is largely bred, numerous to Encarsia formosa batch production Give birth to energetically meaning.
Brief description of the drawings
Fig. 1 is the broad flow diagram of reproductive process of the present invention.
Embodiment
By following embodiments further illustrate description the present invention, do not limit the invention in any way, without departing substantially from On the premise of the technical solution of the present invention, easy to implement any of those of ordinary skill in the art made for the present invention changes Dynamic or change is fallen within scope of the presently claimed invention.In addition, case implementation of the present invention is all big in Jilin agricultural Learn in natural enemy insect industry research and development base Greenhouse and carry out, the implementation time is 4 ~ August in 2017.
Embodiment 1
The implementation case breeds Encarsia formosa using tobacco as check plant, other reproductive processes and saussurea involucrata in addition to the cultivation of tobacco Fruit is consistent, and the implementation case key step is as follows:
(1)The cultivation of healthy host plant
The cultivation of yacon:The nursery in culturing pot by yacon bulb, after 4 leaves are grown after emergence(3 ~ 4 blade monolithic blade faces Product is in 10 ~ 15cm2), it is spare to 8 ~ 12 leaves to be transplanted to cultivation in container.15 ~ 20d, which is poured, in cultivating process applies nutrient solution once (Nutrient composition is urea and Diammonium phosphate (DAP), and ratio used in two kinds of fertilizer is 3:1, carry out 25 ~ 30 times of dilutions.)Every 10 d nursery A collection of, every 10 ~ 15 d transplantings are a collection of, ensure the sufficient supply of plant.The weather conditions of seedling rearing room be 18~28 DEG C, 40 ~ 60% R.H., illumination condition is natural lighting.
The cultivation of tobacco:By tobacco seed(Lucky cigarette 9)The nursery in seedlings nursing plate, tobacco grows the 4th true leaf after emergence When, it is transplanted in container and cultivates spare to 8 ~ 12 true leaves, 15 ~ 20 d, which are poured, in the cultivating process of tobacco applies nutrient solution once(Battalion Nutrient solution component is urea and Diammonium phosphate (DAP), and ratio used in two kinds of fertilizer is 2:1, carry out 25 ~ 30 times of dilutions.).Every 7 ~ 10 d of tobacco Nursery is a collection of, and every 7 ~ 15 d transplantings are a collection of, ensures the sufficient supply of plant.The weather conditions of seedling rearing room are consistent with yacon.
(2)Trialeurodes vaporariorum Westwood kind worm breeds
Trialeurodes vaporariorum Westwood is bred using 8 ~ 12 leaf yacons cultivated, and yacon is placed in cage, and average every plant is released 300-500 Trialeurodes vaporariorum Westwoods are put, when every plant of aleyrodid quantity is up to 3000 ~ 4000 adults, every 20~30 d replaces 1/3 ~ 2/3 Yacon plant(Aleyrodid breeding weather conditions be 20~28 DEG C, 50~70% R.H., 14:10L:D).Worm cage ruler used in breeding Very little size is 2.2m × 2.2m × 1.2m, prevents that the worm net that Trialeurodes vaporariorum Westwood is escaped from being 80 mesh.
(3)Trialeurodes vaporariorum Westwood host nymph largely breeds
The present embodiment step(2)It is loaded with yacon plant and the step of aleyrodid kind worm(1)The yacon plant of cultivation is according to 3:1 into Row inoculation, i.e. every plant of yacon inoculation Trialeurodes vaporariorum Westwood adult 9000 ~ 12000, adult whitefly is removed after being inoculated with 24 h(Utilize Air blower removes adult whitefly, and the adult whitefly of removal continues to breed in the breeding of worm kind), the yacon plant after inoculation is moved Continue to develop to development interior(Trialeurodes vaporariorum Westwood develops room weather conditions:20~28 DEG C, 50~70% R.H., 14:10 L:D), Trialeurodes vaporariorum Westwood more than 70% is developed to spare during 2~3 age on plant.Worm cage size used in breeding for 2.2m × 2.2m × 1.2m, prevents that the worm net that Trialeurodes vaporariorum Westwood is escaped from being 80 mesh.
(4)Encarsia formosa kind bee produces
Encarsia formosa kind bee utilizes the present embodiment step(3)The Trialeurodes vaporariorum Westwood of breeding is produced, this implementation is placed in cage Example step(3)The Trialeurodes vaporariorum Westwood host nymph of breeding(20~28 DEG C, 50~70% R.H. of weather conditions, 14:10 L:D), Every plant of release Encarsia formosa 500 ~ 1000, when every plant containing the black pupa of Encarsia formosa 40000 ~ 60000, every 10 ~ 20d is by 1/ 5 ~ 3/5 plant replaces with the plant that right 4 largely breeds aleyrodid, to ensure Encarsia formosa population quantity.
(5)Encarsia formosa is largely bred
Utilize the present embodiment step(4)The Encarsia formosa kind bee of breeding and the present embodiment step(3)The Trialeurodes vaporariorum Westwood of breeding is posted Main 1:10 connect bee, i.e., the every plant plant with Trialeurodes vaporariorum Westwood host, is inoculated with the Encarsia formosa 8000 ~ 10000 that will sprout wings, Encarsia formosa is removed after being inoculated with 72 h(Encarsia formosa is removed using air blower, the Encarsia formosa of removal can connect bee room or return Receive to honeybee kind breeding room and continue to breed), go after bee to move to development room and continue to develop(Encarsia formosa develops room weather conditions:20~ 28 DEG C, 50~70% R.H., 14:10 L:D), connect the hydromel that Encarsia formosa during bee feeds 10 ~ 15% concentration.Wait to develop To black pupa, and black pupa is obtained after not sprouted wings by parasitic Trialeurodes vaporariorum Westwood nymph, and carry out black pupa collection and preservation.
Test example 1
By 1 result of embodiment and analysis
1st, the comparison of yacon and tobacco plant character
Growth time and main plant character are investigated needed for cultivating the yacon in embodiment 1 and tobacco.
Specific investigation method is as follows:
By 50 yacon bulbs and 50 tobacco seed sowings, the investigation of phase is gone through in the emergence to yacon and tobacco.To saussurea involucrata 8 leaves go through phase, internode away from when being investigated after the leaf area of fruit and tobacco, transplanting, every kind of host plant randomly select 10 plants into Row investigation, leaf area measurement use grid collimation method.
As a result with analysis
It is more than the leaf area of tobacco by counting the leaf area it has been found that yacon single plant is averaged in contrast table 1, internode is away from also greater than cigarette Grass, has greater advantage with respect to the Agronomic trait of tobacco yacon.From 8 blades after the seedling-growing time of two kinds of host plants and transplanting From the point of view of growth time, yacon also has greater advantage compared with tobacco.
The comparison of 1 yacon of table and tobacco section investigation parameter
2nd, the comparison of Trialeurodes vaporariorum Westwood development duration and egg laying amount on yacon and tobacco
Are received by ovum amount and is adjusted for Trialeurodes vaporariorum Westwood 2, the development duration of 3 age nymphs, single plant on yacon in embodiment 1 and tobacco Look into.
Specific investigation method is as follows:
The investigation of development duration:Feelings are once developed in random 100, choosing mark Trialeurodes vaporariorum Westwood ovum, every 12 h observations on yacon Condition, until being developed to 2,3 age nymphs, is repeated 5 times, and the age division of Trialeurodes vaporariorum Westwood is with reference to Huang Yaoge etc.(1993).
The investigation of egg laying amount:Randomly select 5 plants of the present embodiment steps(3)The middle yacon for removing Trialeurodes vaporariorum Westwood adult, The investigation that egg laying amount is carried out using " five point sampling ", every investigation 25cm2, the data of sampling survey and leaf area in proportion into Row conversion obtains single plant and receives ovum amount.
Above section is tested, and the test method of tobacco is consistent with yacon, and the data obtained is above experiment number in table 2 According to average.
As a result with analysis
By being found to the data comparison in table 2, compared with tobacco, it is developed to 2,3 age nymphs in yacon Trialeurodes vaporariorum Westwood Time it is shorter, single plant receive ovum amount it is more advantageous.
The comparison of 2 Trialeurodes vaporariorum Westwood of table part test parameter on yacon and tobacco
Host plant Being developed to for 2 ages goes through the phase(d) Being developed to for 3 ages goes through the phase(d) Single plant receives ovum amount(Ten thousand/plant)
Yacon 8.4 11.0 9.3
Tobacco 9.0 11.7 8.9
3rd, the Trialeurodes vaporariorum Westwood parasitism adaptability that Encarsia formosa breeds two kinds of host plants
The parasitic rate of Trialeurodes vaporariorum Westwood 2 ~ 3 age nymph bred to Encarsia formosa parasitism yacon in embodiment 1 and tobacco, sprout wings Rate and development duration are investigated.
Specific investigation method is as follows:
Mark the present embodiment step(5)Connect the Trialeurodes vaporariorum Westwood after bee 2,3 ages were investigated, randomly select 20 points and taken Sample, each point include 30 Trialeurodes vaporariorum Westwoods first 2 or 3 age nymphs, and every 12 h observes the developmental state of an Encarsia formosa until plumage Change, and count parasitic rate, the eclosion rate of the black pupa number of Encarsia formosa and emergence number calculating Encarsia formosa.To Encarsia formosa development duration Calculated since being inoculated with Encarsia formosa.
As a result with analysis
By the number in contrast table 3 it has been found that Trialeurodes vaporariorum Westwood 2,3 nymph parasitic rates that Encarsia formosa breeds yacon are higher than Tobacco, and to the 3 age nymph parasitic rate highest of Trialeurodes vaporariorum Westwood on yacon.The temperature of the parasitic two kinds of host plants breeding of Encarsia formosa Room trialeurodes vaporariorum nymph eclosion rate difference and unobvious, but the eclosion rate of parasitic yacon 3 age aleyrodid nymph is higher.Encarsia formosa is posted Development duration needed for Trialeurodes vaporariorum Westwood nymph on raw yacon is shorter than tobacco, and the development duration of parasitic 3 age aleyrodid nymphs It was shorter than for 2 ages.Based on the above results, Encarsia formosa has preferable biological characteristics sex expression on yacon.
Parasitic rate, eclosion rate and the development duration of 3 Encarsia formosa parasitism different larval instar aleyrodid nymph of table
Host plant 2 age parasitic rates(%) 3 age parasitic rates(%) 2 age eclosion rates(%) 3 age eclosion rates(%) Parasitic 2 age nymphal developments go through the phase(d) Parasitic 3 age nymphal developments go through the phase(d)
Yacon 75.8 84.2 93.5 94.7 15.5 13.7
Tobacco 75.3 80.3 93.3 93.8 15.9 14.2
Embodiment 2
The present embodiment is same as Example 1, differs only in Encarsia formosa and connects the bee time and is changed to 48 h by 24 h.
Embodiment 3
The present embodiment is same as Example 1, differs only in Encarsia formosa and connects the bee time and is changed to 72 h by 24 h.
Embodiment 1,2,3 receives the analysis of pupa amount
4 embodiment of table, 1 ~ 3 yacon is vector plant, the contrast of the black pupa collecting amount of Encarsia formosa
Each 5 plants of yacons of case are randomly selected in table 4 to be investigated, and investigate single plant Trialeurodes vaporariorum Westwood quantity and black pupa quantity, It is averaged and is calculated using the data of investigation.
By table 4 as can be seen that on yacon embodiment 1 connect 24 h parasitic rates of bee it is minimum receive pupa amount it is minimum, connect bee 72 H parasitic rates have reached 95.3%, and it is maximum to receive pupa amount.
4 yacon of table is vector breeding plant Encarsia formosa
Numbering Aleyrodid quantity(Ten thousand heads/plant) Black pupa amount(Ten thousand heads/plant) Parasitic rate(%)
Case 1 9.0 5.7 63.0
Case 2 8.9 7.3 81.6
Case 3 9.3 8.9 95.3
5 embodiment of table, 1 ~ 3 tobacco is vector plant, the contrast of the black pupa collecting amount of Encarsia formosa
Each 5 plants of tobaccos of case are randomly selected in table 5 to be investigated, and investigate single plant Trialeurodes vaporariorum Westwood quantity and black pupa quantity, profit It is averaged and is calculated with the data of investigation.
By table 5 as can be seen that on cigarette embodiment 1 connect 24 h parasitic rates of bee it is minimum receive pupa amount it is minimum, meet 72 h of bee and post Raw rate has reached 91.4%, and it is maximum to receive pupa amount.
Integrated comparative table 4 and table 5 find that the black pupa amount of the single plant Encarsia formosa on yacon is much larger than tobacco, and yacon The parasitic rate of upper Encarsia formosa is higher, and Encarsia formosa is with connecing the increase parasitic rate of bee time in increasing on two kinds of host plants Big trend, and be that 72 h parasitic rates are optimal.
5 tobacco of table is vector breeding plant Encarsia formosa
Numbering Aleyrodid quantity(Ten thousand heads/plant) Black pupa amount(Ten thousand heads/plant) Parasitic rate(%)
Case 1 8.5 4.7 55.5
Case 2 8.3 6.4 77.3
Case 3 8.8 8.0 91.4
Conclusion:The investigation and analysis of integrated embodiment 1 ~ 3 it turns out that, it is small largely to breed beautiful aphid using yacon as vector plant Bee, is greatly decreased the cultivation time needed for host plant;The development duration of Trialeurodes vaporariorum Westwood is shortened at the same time, and it is white to add greenhouse The egg laying amount of aleyrodid;Also the development duration of Encarsia formosa is shortened, the yield of the black pupa of single plant Encarsia formosa is improved, with yacon Largely breed Encarsia formosa for vector plant has greater advantage compared with tobacco.
Although the embodiment of the present invention is the foregoing described, it will be appreciated by those of skill in the art that this is only It is merely illustrative of, protection scope of the present invention is defined by the appended claims, and those skilled in the art is not carrying on the back On the premise of from the principle of the present invention and essence, various changes or modifications can be made to these embodiments, these change and Modification each falls within protection scope of the present invention.

Claims (6)

  1. A kind of 1. method that Encarsia formosa is largely bred for host plant based on yacon, it is characterised in that:Using yacon in Between host plant, using Trialeurodes vaporariorum Westwood as host, the batch production for carrying out Encarsia formosa is largely bred:
    (1)The cultivation of healthy yacon plant;
    (2)Trialeurodes vaporariorum Westwood kind worm breeds;
    (3)Trialeurodes vaporariorum Westwood host nymph largely breeds;
    (4)Encarsia formosa kind bee produces;
    (5)Encarsia formosa is largely bred.
  2. 2. a kind of method for largely breeding Encarsia formosa based on yacon for host plant according to claim 1, it is special Sign is:
    Step(1)Described in yacon bulb nursery is carried out in culturing pot, be transplanted to after emergence in container and cultivate to 8 ~ 12 blades, the every 10 d nursery of yacon seedling is a collection of, and every 10 ~ 15 d transplantings are a collection of, ensures the sufficient supply of plant.
  3. 3. a kind of method for largely breeding Encarsia formosa based on yacon for host plant according to claim 1, it is special Sign is:
    Step(2)Described in Trialeurodes vaporariorum Westwood bred using the yacon plant in right 2, yacon is placed in cage Plant, 300 ~ 500 Trialeurodes vaporariorum Westwoods of average every plant of release, when every plant of aleyrodid quantity is up to 3000 ~ 4000 adults, every 15~ 30 d replace 1/3 ~ 2/3 yacon plant.
  4. 4. a kind of method for largely breeding Encarsia formosa based on yacon for host plant according to claim 1, it is special Sign is:
    Step(3)Described in a large amount of breeding Trialeurodes vaporariorum Westwood host nymphs, the saussurea involucrata of aleyrodid kind worm will be loaded with claim 3 Fruit plant is with the yacon plant in right 2 according to 3:1 is inoculated with, and adult whitefly is removed after being inoculated with 24 h, after inoculation Yacon plant moves to development interior and continues to develop, and Trialeurodes vaporariorum Westwood more than 70% is developed to spare during 2~3 age in plant to be planted.
  5. 5. a kind of method for largely breeding Encarsia formosa based on yacon for host plant according to claim 1, it is special Sign is:
    Step(4)Described in Encarsia formosa kind bee bred using the aleyrodid in right 4, Trialeurodes vaporariorum Westwood is placed in cage Host nymph, average every plant of release Encarsia formosa 500 ~ 1000, treat every plant containing the black pupa 40000 of Encarsia formosa that will sprout wings ~ At 60000,1/5 ~ 3/5 plant is replaced with the plant that right 4 largely breeds aleyrodid by every 10 ~ 20d, to ensure Encarsia formosa Population quantity.
  6. 6. a kind of method for largely breeding Encarsia formosa based on yacon for host plant according to claim 1, it is special Sign is:
    Step(5)Described in Encarsia formosa a large amount of breedings, utilize the Encarsia formosa kind bee that right 5 is bred and claim 4 The Trialeurodes vaporariorum Westwood host of breeding is by benefit evil ratio 1:10 connect bee, and Encarsia formosa is removed after being inoculated with 72 h, and development room is moved to after going bee Continue to develop, wait to be developed to black pupa, carry out black pupa collection and preservation.
CN201711313801.3A 2017-12-12 2017-12-12 Method for breeding encarsia formosa massively based on yacon as host plant Expired - Fee Related CN107926868B (en)

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Cited By (2)

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Publication number Priority date Publication date Assignee Title
CN110338154A (en) * 2019-08-09 2019-10-18 河南科技大学 For breeding vector plant system and construction method, the propagation method and the method for preventing and treating aleyrodid class pest of pale yellow grace aphid chalcid fly
CN111527950A (en) * 2020-04-26 2020-08-14 无锡南长园林绿化工程有限公司 Prevention and control method for goldenrain tree aphids

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