CN107926868B - Method for breeding encarsia formosa massively based on yacon as host plant - Google Patents
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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Abstract
The invention discloses a method for breeding encarsia formosa massively based on yacon as a host plant, and relates to a new method for breeding encarsia formosa, wherein the yacon is used as an intermediate host plant, and greenhouse trialeurodes vaporariorum is used as a host to carry out industrial massively breeding of the encarsia formosa: (1) cultivating healthy yacon plants; (2) breeding the greenhouse trialeurodes vaporariorum; (3) breeding a large number of greenhouse trialeurodes vaporariorum host nymphs; (4) producing encarsia formosa; (5) and breeding the encarsia formosa in a large quantity. The method takes the yacon as an intermediate host plant to breed the encarsia formosa in a large quantity, shortens the breeding period of the trialeurodes vaporariorum and the encarsia formosa in a greenhouse, further improves the quality of breeding the encarsia formosa in a large quantity, and has positive significance for the industrial breeding of the encarsia formosa. The breeding period of the trialeurodes vaporariorum and the encarsia formosa further improves the quality of mass breeding of the encarsia formosa, and has positive significance for industrial breeding of the encarsia formosa.
Description
Technical Field
The invention discloses a method for breeding encarsia formosa massively based on yacon as a host plant, relates to a new method for breeding encarsia formosa, and belongs to the field of biological control of agricultural and forestry pests.
Background
Whiteflies (Hemiptera), the family Aleyrodidae (Aleyrodidae), are one of the important pests in agricultural production. Biological control of whitefly pests is considered to be the most safe and effective control means at present. Use of Encarsia formosaEncarsia formosa Gahan for preventing and controlling bemisia tabaciBemisia tabaci(Gennadius) and greenhouse whiteflyTrialeurodes vaporariorum(Westwood) is considered as one of the most classical control cases of biological control, since Encarsia formosa was introduced from the United kingdom in 1978, China made a great deal of first-pass research and organized relevant units to produce and release, and released Encarsia formosa controlled greenhouse whitefly and Bemisia tabaci also achieved significant control effects compared with chemical control. However, the yield of the encarsia formosa still cannot meet the large demand in agricultural production.
At present, the encarsia formosa is bred by a five-chamber bee breeding method by mainly using greenhouse whitefly and bemisia tabaci as hosts and using tomatoes, tobaccos and the like as intermediate host plants. However, the existing factory breeding of encarsia formosa also has a plurality of problems, so that the breeding efficiency of the encarsia formosa still needs to be further improved. Due to the difference between the breeding host and the intermediate host plant of the encarsia formosa, the quality of the bred encarsia formosa is also greatly different. Therefore, the main reason for influencing the encarsia formosa mainly lies in the species of breeding hosts and the species of intermediate host plants, and the combination of the prior research finds that the greenhouse trialeurodes vaporariorum is the dominant host of the encarsia formosa, while the current intermediate host species are more, wherein the tobacco is considered as the best intermediate host plant for breeding the encarsia formosa, but the problem that the tobacco breeding the encarsia formosa has more problems, and the search for a new intermediate host plant is the key for improving the breeding efficiency of the encarsia formosa.
Yacon belongs to Compositae (Asteraceae), sunflower (Helianthus), dicotyledonous herbaceous root-tuber plants, the plant characteristics are excellent, the plant height is large, the plant height can be more than 2 meters, the pitch is larger, the leaves are paired, the number of the leaves of a single plant can reach 20, the area of the single leaf can reach 500cm at most2The above. In recent years, with the success of introduction of yacon in China, the planting area is also getting larger and larger. The yacon planting is simple and easy to manage, and has various breeding modes, the yacon can be bred by utilizing seed balls, axillary buds and plant division, and a single plant can produce more than 10 seed balls and more than 30 axillary buds. Compared with tobacco, the plant diseases and insect pests of the yacon are less, and the greenhouse trialeurodes vaporariorum is one of the important pests in the production of the yacon. At present, yacon plants and root tubers are mainly developed and researched by using food and extract samples, but no related research report exists on the development and utilization of insects taking the yacon plants as host resources.
Disclosure of Invention
The invention provides a method for breeding encarsia formosa massively based on yacon as a host plant, which is characterized by comprising the steps of obtaining yacon plants, breeding greenhouse trialeurodes vaporariorum and breeding encarsia formosa massively by taking the yacon as an intermediate host plant and the greenhouse trialeurodes vaporariorum as a host.
The invention relates to a method for breeding a large number of encarsia formosa based on yacon as a host plant, which is characterized by comprising the following steps: taking yacon as an intermediate host plant and greenhouse trialeurodes vaporariorum as a host, carrying out industrial mass breeding on encarsia formosana:
(1) cultivating healthy yacon plants;
(2) breeding the greenhouse trialeurodes vaporariorum;
(3) breeding a large number of greenhouse trialeurodes vaporariorum host nymphs;
(4) producing encarsia formosa;
(5) and breeding the encarsia formosa in a large quantity.
And (2) seedling the yacon seed balls in the step (1) in a culture bowl, transplanting the yacon seed balls into a container after seedling emergence, and culturing the yacon seed balls until 8-12 leaves are obtained, wherein the yacon seed balls are transplanted one batch every 10 days and one batch every 10-15 days, so that sufficient supply of plants is ensured.
The greenhouse trialeurodes vaporariorum in the step (2) is bred by using the yacon plants in the step (2), the yacon plants are placed in a cage, 300-500 heads of greenhouse trialeurodes vaporariorum is released by each plant averagely, and 1/3-2/3 yacon plants are replaced every 15-30 days when the number of the trialeurodes vaporariorum reaches 3000-4000 adults. (the climatic conditions for the breeding of the whiteflies are 20-28 ℃ and 50-70% of R.H., 14:10L: D).
And (3) breeding a large amount of greenhouse trialeurodes vaporariorum host nymphs, inoculating the yacon plants carrying the trialeurodes vaporariorum in the step (2) and the yacon plants in the step (1) according to a ratio of 3:1, namely inoculating 9000-12000 heads of greenhouse trialeurodes vaporariorum imagoes to each yacon plant, removing the trialeurodes vaporariorum imagoes after inoculating for 24 h (removing the trialeurodes vaporariorum imagoes by using an air blower, continuously breeding the removed trialeurodes vaporariorum imagoes in breeding of the insect seeds), transferring the inoculated yacon plants to a development room to continuously develop (climate conditions in the development room of the greenhouse trialeurodes vaporariorum: 20-28 ℃, 50-70% of R.H., 14:10L: D), and keeping more than 70% of the greenhouse trialeurodes vaporariorum on.
And (3) breeding the encarsia formosa by using the whiteflies in the step (3), placing the bred greenhouse trialeurodes vaporariorum host nymphs (the climate conditions are 20-28 ℃, 50-70% R.H., 14:10L: D) in the cage, averagely releasing 500-1000 heads of the encarsia formosa per plant, and replacing 1/5-3/5 plants with the plants bred with whiteflies in the step (3) every 10-20 days when each plant contains black pupas 40000-60000 heads of the encarsia formosa to be eclosized so as to ensure the population number of the encarsia formosa.
And (5) massively breeding the encarsia formosa, namely, breeding the encarsia formosa bred in the step (4) and breeding the greenhouse trialeurodes vaporariorum host 1: 10, inoculating the bees, removing the encarsia formosa after 72 h of inoculation (the encarsia formosa can be removed by an air blower and can be continuously bred in an inoculation chamber or recovered to a bee species breeding chamber), removing the bees, moving to a development chamber for continuous development (the climatic conditions of the encarsia formosa development chamber are 20-28 ℃, 50-70% of R.H., and 14:10L of D), obtaining black pupas after the encarsia formosa is developed to black pupas and is not eclosized by parasitized greenhouse trialeurodes vaporariorum nymphs, and collecting and storing the black pupas.
Positive significance of the invention
The invention has the positive effects that:
the pest and disease damage is less in the yacon cultivation process, and the yacon breeding method is simple; compared with tobacco, the cultivation period of 8-12 leaves of yacon is shorter, and the cultivation time of intermediate host plants is greatly shortened; the yacon leaves have larger area, so that the sufficient egg laying space of whitefly and encarsia formosa can be ensured; in addition, the pitch space of the yacon is larger, which is beneficial for the greenhouse whitefly and the encarsia formosa to receive light, thereby promoting the development of the encarsia formosa.
Compared with tobacco, the yacon is more suitable for the development and reproduction of greenhouse trialeurodes vaporariorum, the development period of the greenhouse trialeurodes vaporariorum on the yacon is further shortened, and the egg laying amount is further increased.
The encarsia formosa has better adaptability to greenhouse trialeurodes vaporariorum bred by the yacon, the development duration of the greenhouse trialeurodes vaporariorum bred by the encarsia formosa parasitic yacon is shorter, the parasitism rate and the emergence rate are higher, and the encarsia formosa parasitic yacon has better biocontrol potential.
In conclusion, the method takes the yacon as an intermediate host plant to breed the encarsia formosa in large quantity, shortens the breeding period of the trialeurodes vaporariorum and the encarsia formosa in a greenhouse, further improves the quality of breeding the encarsia formosa in large quantity, and has positive significance for the factory breeding of the encarsia formosa.
Drawings
FIG. 1 is a main flow chart of the breeding process of the present invention.
Detailed Description
The present invention is further illustrated by the following examples, which do not limit the present invention in any way, and any modifications or changes that can be easily made by a person skilled in the art to the present invention will fall within the scope of the claims of the present invention without departing from the technical solution of the present invention. In addition, the implementation of the method is carried out in a facility greenhouse of a natural enemy insect industry research and development base of Jilin agriculture university, and the implementation time is 4-8 months in 2017.
Example 1
The implementation case takes tobacco as a control plant to breed the encarsia formosa, the breeding processes except the cultivation of the tobacco are consistent with those of yacon, and the implementation case mainly comprises the following steps:
(1) cultivation of healthy host plants
Culturing yacon: seedling the yacon seed balls in a culture bowl, and growing 4 leaves after seedling emergence (the area of a single leaf of 3-4 leaves is 10-15 cm)2) And transplanting the leaves into a container to cultivate 8-12 leaves for later use. And (2) irrigating the nutrient solution once every 15-20 days in the cultivation process (the nutrient solution comprises urea and diammonium phosphate, the ratio of the two fertilizers is 3:1, and the two fertilizers are diluted by 25-30 times), cultivating seedlings one batch every 10 days, and transplanting one batch every 10-15 days to ensure sufficient supply of plants. The climate conditions of the seedling raising room are 18-28 ℃, 40-60% of R.H., and the illumination conditions are natural illumination.
Culturing tobacco: tobacco seeds (Jiyan No. 9) are grown in a seedling raising tray, when 4 th true leaf of tobacco grows out after emergence of seedlings, the tobacco is transplanted into a container to be cultured until 8-12 true leaves are reserved, and nutrient solution is poured once (the nutrient solution comprises urea and diammonium phosphate, the ratio of the two fertilizers is 2:1, and the dilution is carried out by 25-30 times) in 15-20 days in the tobacco culturing process. One batch of tobacco seedlings is grown every 7-10 days, and one batch of tobacco seedlings is transplanted every 7-15 days, so that sufficient supply of plants is guaranteed. The climate condition of the seedling raising room is consistent with that of the yacon.
(2) Breeding of trialeurodes vaporariorum in greenhouse
The greenhouse trialeurodes vaporariorum is bred by using 8-12 cultivated leaf smallanthus sonchifolius, the smallanthus sonchifolius is placed in a cage, 500 greenhouse trialeurodes vaporariorum is released by each plant averagely, and 1/3-2/3 smallanthus sonchifolius plants are replaced every 20-30 days when the number of each plant trialeurodes vaporarum reaches 3000-4000 adults (the climatic conditions for breeding the trialeurodes vaporariorum are 20-28 ℃, 50-70% of R.H., and 14:10L: D). The size of the insect cage for breeding is 2.2m multiplied by 1.2m, and the insect net for preventing the greenhouse trialeurodes vaporariorum from escaping is 80 meshes.
(3) Mass breeding of greenhouse trialeurodes vaporariorum host nymphs
In the embodiment, the yacon plants carrying the whitefly plantlets in the step (2) and the yacon plants cultivated in the step (1) are inoculated according to a ratio of 3:1, namely each yacon is inoculated with 9000-12000 heads of greenhouse whitefly adults, the whitefly adults are removed after inoculation for 24 hours (the whitefly adults are removed by an air blower and continuously bred in breeding of the plantlets), the inoculated yacon plants are moved to a development room to continue development (the climatic conditions of the greenhouse whitefly development room are 20-28 ℃, 50-70% of R.H., 14:10L: D), and more than 70% of greenhouse whitefly on the plants are developed to 2-3 years for later use. The size of the insect cage for breeding is 2.2m multiplied by 1.2m, and the insect net for preventing the greenhouse trialeurodes vaporariorum from escaping is 80 meshes.
(4) Production of encarsia formosa species
The Encarsia formosa is produced by utilizing greenhouse whiteflies bred in the step (3) in the embodiment, the greenhouse whitefly host nymphs bred in the step (3) in the embodiment (the climate conditions are 20-28 ℃, 50-70% R.H., 14:10L: D) are placed in a cage, 500-1000 heads of the Encarsia formosa are released from each plant, and when each plant contains enccarsia formosa black pupa 40000-60000, 1/5-3/5 plants are replaced by plants breeding a large amount of whiteflies in the range of 4 in 10-20 days, so that the population number of the Encarsia formosa is guaranteed.
(5) Mass propagation of encarsia formosa
The encarsia formosa bred in the step (4) of the embodiment and the greenhouse whitefly host 1 bred in the step (3) of the embodiment are used: 10 inoculating bees, namely inoculating 8000-10000 heads of encarsia formosa to be eclosized to each plant with a greenhouse trialeurodes vaporariorum host, removing encarsia formosa after inoculating for 72 hours (the encarsia formosa is removed by an air blower, the removed encarsia formosa can be removed in a bee inoculation chamber or recycled to a bee species breeding chamber for continuous breeding), moving the encarsia formosa to a breeding chamber for continuous development (the climatic conditions of the encarsia formosa development chamber are 20-28 ℃, 50-70% of R.H., 14:10L: D), and feeding 10-15% of honey water to the encarsia formosa in the bee inoculation process. And obtaining black pupae after the black pupae develop and are eclosized by the greenhouse trialeurodes vaporariorum nymphs which are not parasitized, and collecting and storing the black pupae.
Test example 1
The results and analysis of example 1
1. Comparison of the botanical Properties of yacon and tobacco
The growth time and the main botanical properties required for the cultivation of yacon and tobacco in example 1 were investigated.
The specific investigation method is as follows:
sowing 50 yacon seed balls and 50 tobacco seeds, and investigating the seedling emergence period of the yacon and the tobacco. When leaf areas, 8 leaf periods after transplanting, internode intervals and the like of the yacon and the tobacco are investigated, 10 plants of each host plant are randomly selected for investigation, and a grid line method is adopted for measuring the leaf areas.
Results and analysis
The data in the table 1 show that the average leaf area of the single plant of the yacon is larger than that of the tobacco, the internode distance is also larger than that of the tobacco, and the yacon has great advantages compared with the botanical property of the tobacco. From the time of emergence of seedlings and the time of growth of 8 leaves after transplantation of two host plants, the yacon also has great advantages compared with tobacco.
TABLE 1 comparison of partial survey parameters for yacon and tobacco
2. Comparison of development duration and oviposition amount of greenhouse trialeurodes vaporariorum on yacon and tobacco
The development history and the single-plant egg collection amount of nymphs of 2 and 3 years old of greenhouse trialeurodes vaporariorum in the yacon and the tobacco in example 1 were examined.
The specific investigation method is as follows:
investigation of development history: randomly selecting and marking 100 greenhouse whitefly eggs on yacon, observing the development condition every 12 h until 2-3-year nymphs develop, repeating for 5 times, and referring to yellow pavilion and the like for the age classification of greenhouse whitefly (1993).
Examination of egg laying amount: randomly selecting 5 yacon plants from which adult greenhouse whitefly was removed in step (3) of the present example, and investigating egg laying amount by a five-point sampling method, wherein each point investigates 25cm2And converting the data of sampling investigation and the leaf area according to a proportion to obtain the egg yield of the single plant.
In the above part of experiments, the test method of tobacco is consistent with that of yacon, and the data obtained in table 2 are the average of the above test data.
Results and analysis
The comparison of data in the table 2 shows that compared with tobacco, the time for the trialeurodes vaporariorum to develop to 2 and 3 instars nymphs in the yacon greenhouse is shorter, and the egg yield of each plant is more advantageous.
TABLE 2 comparison of partial test parameters of Trialeurodes vaporariorum on yacon and tobacco in greenhouse
| Host plant | Development to 2 years old (d) | Development to 3 years old (d) | Egg yield per plant (ten thousand grains per plant) |
| Yacon fruit | 8.4 | 11.0 | 9.3 |
| Tobacco | 9.0 | 11.7 | 8.9 |
3. Adaptability of encarsia formosa to parasitism of two kinds of host plants for breeding greenhouse trialeurodes vaporariorum
The parasitic rate, emergence rate and development duration of 2-3-year nymphs of greenhouse trialeurodes vaporariorum bred by encarsia formosa parasitic yacon and tobacco in example 1 are investigated.
The specific investigation method is as follows:
marking the greenhouse whitefly of 2 and 3 ages after bee inoculation in the step (5) of the embodiment for investigation, randomly selecting 20 points for sampling, wherein each point comprises 30 greenhouse whitefly head nymphs of 2 or 3 ages, observing the development condition of the encarsia formosa every 12 hours until emergence, and counting the number of black pupae and the number of emergence of the encarsia formosa to calculate the parasitic rate and the emergence rate of the encarsia formosa. The growth history of encarsia formosa is calculated from the inoculation of encarsia formosa.
Results and analysis
The data in the table 3 are compared to find that the parasitism rate of the encarsia formosa on greenhouse trialeurodes vaporariorum 2 and 3 bred by the smallfruit avenae is higher than that of tobacco, and the parasitism rate of the encarsia formosa on greenhouse trialeurodes vaporariorum 3 on the smallfruit avenae is highest. The difference of emergence rates of greenhouse whitefly nymphs bred by two host plants parasitized by encarasites of encarsia formosa is not obvious, but the emergence rate of the whitefly nymphs of 3-year-old smallanthus sonchifolius is higher. The development duration of greenhouse whitefly nymphs on the encarsia formosa parasitism smallanthus sonchifolius is shorter than that of tobacco, and the development duration of the 3 th instar whitefly nymphs parasitizing the encarsia formosa is shorter than that of the 2 th instar nymphs. The combination of the results shows that the encarsia formosa has better biological characteristic performance on the yacon.
TABLE 3 parasitism rate, emergence rate and development duration of different instars of whitefly nymphs parasitized by encarsia formosa
| Host plant | Parasitic rate at 2 years (%) | Parasitic rate at 3 years (%) | Eclosion Rate at 2 years old (%) | 3-instar eclosion Rate (%) | Parasitic 2-year nymph development calendar (d) | Parasitic 3 years nymph development calendar (d) |
| Yacon fruit | 75.8 | 84.2 | 93.5 | 94.7 | 15.5 | 13.7 |
| Tobacco | 75.3 | 80.3 | 93.3 | 93.8 | 15.9 | 14.2 |
Example 2
This example is identical to example 1, except that the time for the encarsia formosa to receive the encarsia formosa is changed from 24 h to 48 h.
Example 3
This example is identical to example 1, except that the time for the encarsia formosa to receive the encarsia formosa is changed from 24 h to 72 h.
Examples 1, 2 and 3 analysis of pupa collection amount
TABLE 4 comparison of the amount of collected black pupae of Encarsia formosa, examples 1 to 3, with yacon as intermediate host plant
In table 4, 5 yacon plants were randomly selected for each case to investigate the number of trialeurodes vaporariorum and the number of black pupae in a single greenhouse, and the average number was calculated by using the investigated data.
As can be seen from Table 4, in example 1 of yacon, the parasitic rate of bee inoculation for 24 h is the lowest, the pupa receiving amount is the least, the parasitic rate of bee inoculation for 72 h reaches 95.3%, and the pupa receiving amount is the largest.
TABLE 4 yacon as intermediate host plant for breeding encarsia formosa
| Numbering | Number of whiteflies (ten thousand heads/plants) | Black pupa volume (Wantou/Lizhu) | Parasitic ratio (%) |
| Case 1 | 9.0 | 5.7 | 63.0 |
| Case 2 | 8.9 | 7.3 | 81.6 |
| Case 3 | 9.3 | 8.9 | 95.3 |
TABLE 5 comparison of tobacco used as intermediate host plant and the amount of collected black pupae of Encarsia formosa in examples 1 to 3
In table 5, 5 tobacco plants were randomly selected for each case to investigate the quantity of trialeurodes vaporariorum and the quantity of black pupae in a single greenhouse, and the average number was calculated by using the investigated data.
As can be seen from the table 5, in the embodiment 1 on cigarette, the parasitic rate of the bee-receiving is the lowest and the pupa-receiving amount is the least in 24 h, the parasitic rate of the bee-receiving is up to 91.4 h, and the pupa-receiving amount is the largest.
Comprehensive comparison of tables 4 and 5 shows that the amount of black pupae of a single plant of encarsia formosa on yacon is far greater than that of tobacco, the parasitic rate of encarsia formosa on yacon is higher, the parasitic rates of encarsia formosa on two host plants increase along with the increase of the inoculation time, and the parasitic rates are both the 72-hour parasitic rates are the best.
TABLE 5 tobacco as intermediate host plant for breeding encarsia formosa
| Numbering | Number of whiteflies (ten thousand heads/plants) | Black pupaVolume (Wantou/strain) | Parasitic ratio (%) |
| Case 1 | 8.5 | 4.7 | 55.5 |
| Case 2 | 8.3 | 6.4 | 77.3 |
| Case 3 | 8.8 | 8.0 | 91.4 |
And (4) conclusion: the investigation and analysis results of the comprehensive embodiments 1-3 show that the yacon is used as an intermediate host plant to breed a large amount of encarsia formosa, so that the cultivation time required by the host plant is greatly reduced; meanwhile, the development period of the greenhouse trialeurodes vaporariorum is shortened, and the oviposition amount of the greenhouse trialeurodes vaporariorum is increased; the development period of encarsia formosa is shortened, the yield of the black pupa of a single encarsia formosa is increased, and the great advantage of breeding encarsia formosa by taking yacon as an intermediate host plant is achieved compared with tobacco.
Although specific embodiments of the present invention have been described above, it will be understood by those skilled in the art that this is by way of illustration only, the scope of the present invention being defined by the appended claims, and that various changes or modifications may be made to these embodiments by those skilled in the art without departing from the principle and spirit of the present invention, and these changes and modifications are within the scope of the present invention.
Claims (1)
1. A method for breeding a large number of encarsia formosa based on yacon as a host plant is characterized by comprising the following steps:
(1) cultivation of healthy yacon plants
Seedling raising is carried out on yacon seed balls in a culture bowl, the yacon seed balls are transplanted into a container after seedling emergence, and the yacon seed balls are cultured to 8-12 leaves;
(2) breeding of trialeurodes vaporariorum in greenhouse
Placing the healthy yacon plants cultured in the step (1) in a cage, averagely releasing 300-500 greenhouse trialeurodes vaporariorum per plant, and replacing 1/3-2/3 the healthy yacon plants cultured in the step (1) every 15-30 days when the number of the greenhouse trialeurodes vaporariorum per plant reaches 3000-4000 adults;
(3) mass breeding of greenhouse trialeurodes vaporariorum host nymphs
Inoculating the yacon plant carrying the greenhouse trialeurodes vaporariorum and the healthy yacon plant cultured in the step (1) according to a ratio of 3:1, removing the greenhouse trialeurodes vaporariorum imagoes after inoculating for 24 hours, transferring the inoculated yacon plant to a development room to continue to develop, and keeping standby when more than 70% of the greenhouse trialeurodes vaporariorum on the inoculated yacon plant develops to 2-3 years old;
(4) production of encarsia formosa species
Breeding the encarsia formosa by utilizing greenhouse trialeurodes vaporariorum, placing greenhouse trialeurodes vaporariorum host nymphs in a cage, averagely releasing 500-1000 heads of encarsia formosa for each plant, and replacing a large number of plants for breeding greenhouse trialeurodes vaporariorum with healthy 1/5-3/5 plants cultured in the step (1) every 10-20 days when each plant contains black pupas 40000-60000 of the encarsia formosa to be eclosized, so as to ensure the population quantity of the encarsia formosa;
(5) mass propagation of encarsia formosa
And (3) mixing the bred encarsia formosa and the bred greenhouse trialeurodes vaporariorum host according to the benefit-to-pest ratio of 1: 10 inoculating the bees, removing the encarsia formosa after inoculating for 72 h, moving the encarsia formosa to a development room for continuous development after the encarsia formosa is removed, and collecting and storing the black pupae after the black pupae are developed.
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| CN103385220B (en) * | 2013-07-25 | 2014-10-29 | 任伟 | Scale breeding method for encarsia formosa |
| CN103766289B (en) * | 2014-01-22 | 2015-04-01 | 云南省烟草公司玉溪市公司 | Method for improving breeding efficiency of encarsia formosa |
| CN105340836A (en) * | 2015-11-27 | 2016-02-24 | 山东省农业科学院植物保护研究所 | Method for improving factory propagation efficiency of encarsia formosa |
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