CN100452964C - Steps for breeding high production southern yew to increase pacilitaxel through directional inducement - Google Patents

Steps for breeding high production southern yew to increase pacilitaxel through directional inducement Download PDF

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CN100452964C
CN100452964C CNB2006100105193A CN200610010519A CN100452964C CN 100452964 C CN100452964 C CN 100452964C CN B2006100105193 A CNB2006100105193 A CN B2006100105193A CN 200610010519 A CN200610010519 A CN 200610010519A CN 100452964 C CN100452964 C CN 100452964C
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southern enqlish
enqlish yew
taxol
yew
southern
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CN1914994A (en
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祖元刚
杨逢建
张学科
苑占宇
庞海河
王纪坤
于景华
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Zhejiang Hisun Pharmaceutical Co Ltd
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Zhejiang Hisun Pharmaceutical Co Ltd
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
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    • Y02P60/40Afforestation or reforestation

Abstract

A directional inducing method for culturing the southern enqlish yew in order to increase the yield of taxol includes such steps as choosing proper saplings of enqlish yew, close planting, sunshading properly for 2 years, and increasing the light transmission rate in the third year for inducing the synthesis of taxol.

Description

A kind of southern enqlish yew high-yield cultivating measure of directional induction taxol increment
Technical field:
The invention belongs to the agricultural planting field of new and high technology, particularly a kind of southern enqlish yew high-yield cultivating measure of directional induction taxol increment.
Background technology:
Southern enqlish yew (Taxus Walichiana var.mairei) belongs to taxaceae, Chinese yew genus plants, and the southern enqlish yew young growth stage belongs to feminine tree.From southern enqlish yew, separate obtaining having remarkable anticancer active constituent-taxol and be used for the treatment of multiple cancers such as breast cancer, oophoroma, non-small cell lung cancer, colon cancer, determined curative effect, side effect is less.Therefore the artificial cultivation economic and social benefit that develops southern enqlish yew is remarkable.
Because southern enqlish yew belongs to country-level wild protective plant, resource scarcity for great demand, can only develop cultivation, and at present, according to disclosed patent and bibliographical information, the method for planting southerm yew has following several:
Publication number is that the patent of CN1774987 has been introduced a kind of southern Chinese year potted landscpe cultivating method, this method has been introduced technology such as utilizing soil, seedling seed selection, soil application, seedling foster in the potted landscape cultivation southern enqlish yew, transplanting, the advantage of this method is that the southern enqlish yew of field planting has been transplanted in the flowerpot, has reduced management difficulty.But should invention only southern enqlish yew as ornamental plants, influenced the normal growth of southern enqlish yew, reduced biomass.
Publication number is that the patent of CN1759653 has been introduced a kind of method for planting southerm yew, this method has mainly been introduced seeding and seedling raising and the technical problem of cultivating afforestation in the planting southerm yew, realized that the southern enqlish yew science is grown seedlings, large tracts of land is cultivated fast, improve survival rate, increased the southern enqlish yew resource.This method has just realized the large tracts of land cultivation, does not relate to the southern enqlish yew fast growing, belongs to southern enqlish yew resource breeding phase, does not also relate to the problem that improves content of taxol.
Publication number is that the patent of CN1714620 has been introduced a kind of method that improves content of taxol in the cultivating Chinese yew, and this method is to apply to contain lanthanum compound as fertilizer sources additive to improve content of taxol in the soil of Chinese yew root.This method has only been paid attention to content of taxol, does not pay close attention to the biomass of Chinese yew, therefore can not improve the output of taxol, and this method is that the applied chemistry method improves content of taxol, is not the content that Applied Biotechnology improves taxol.
Publication number is that the patent of CN1685832 has been introduced a kind of method that improves content of taxol in the Chinese yew, this method is to spray coronatine on the cauline leaf of Young Taxus cuspidata Sieb. Et Zucc, though this method can increase substantially the taxol output of cultivating Chinese yew, but this method also is to utilize chemical means to improve the content of taxol, do not introduce the cultural operations of Chinese yew yet, particularly improve the cultural operations of southern enqlish yew biomass in the unit are.
Publication number has been introduced the kind method for planting of a kind of Chinese yew for the CN1615682 patent; this method has been introduced the process that seed collection, selection are grown seedlings ground, site preparation and fertilization, presprouting of seeds, field management and survived the winter and protect; and introduced cuttage and seedling culture method; this method has solved the artificial method for planting problem of Chinese yew; also belong to the problem that taxus resource is cultivated, do not relate to and improve biomass and taxol output category.
Publication number is the artificial cultivation method that the patent of CN1442036 has been introduced a kind of Chinese yew, this method introduces in the Chinese yew plantation items such as fertilizer, water content and transplanting in the soil, the advantage of this method is to cultivate Chinese yew in a large number, solve resource problem in imminent danger, but Chinese yew is a kind of endangered plants of treasuring that huge medical value is arranged, this method does not solve increases unit are Chinese yew biomass, improves the problem of taxol output.
Southern enqlish yew is because its resource is rare especially, medical value is bigger, related science research report is more, mainly concentrate on the southern enqlish yew population structure, raising technology, the Physiological Ecology characteristic, reproduction, the research of aspects such as taxol extractive technique, the southern enqlish yew forest culture and management Study on Technology on forestry science and technology, delivered in September, 1999 of only limiting to about southern enqlish yew cultivation aspect, June calendar year 2001 is at the southern enqlish yew forest plantation cultivation of delivering in the forestry science and technology communication and Study on Growth and the minority bibliographical informations such as southern enqlish yew sylvan life seedling method delivered on the forestry practical technique in April, 2002, the common feature of these documents is the seed collecting of mainly having introduced southern enqlish yew, vernalization, sowing, nursery bed management, measure such as plant, do not relate to the method for taxol increment and southern enqlish yew high yield in the directional induction southern enqlish yew, and the southern enqlish yew in these bibliographical informations goes out garden afforestation damage by disease and insect incidence height, difficult management, production cost is bigger.
Summary of the invention:
The object of the invention is the bioecology characteristic according to southern enqlish yew, the southern enqlish yew cultivation method that provides a kind of southern enqlish yew high-yield cultivating and directional induction taxol to increase, carry out the southern enqlish yew high-yield culturing at China's subtropical forest zones, building for the Chinese commodity woods provides new model.
In order to achieve the above object, the technical solution used in the present invention comprises: belong to feminine tree according to southern enqlish yew nursery stock and young growth stage, normal growth need shade, and the characteristic that environment conditioning can induce content of taxol to improve, southern enqlish yew is shaded in early days, promote biomass to increase, changed the shading condition on the 3rd year, increase light transmittance, induce content of taxol to improve, according to different terrain conditions, the classification plantation is taked less than 30 ° mountain region in the level land and the gradient simultaneously with suiting measures to local conditions, make full use of environment space and land resources on the ground.
The used seedling height of southern enqlish yew is greater than 25cm, and leading thread reaches 0.30cm, and well developed root system is preced with a complete strong sprout.
Southern enqlish yew plant the time then by the end of November to March in next year, before the middle ten days.
Southern enqlish yew adopts the seedbed mode to plant on the level land, seedbed width 150cm, and the gradient adopts solid growth less than 30 ° mountain region, banded afforestation, the afforestation band is vertical with contour, bandwidth 200~400cm.
Southern enqlish yew is at level land line-spacing 30~40cm, spacing in the rows 20~30cm, mountain region line-spacing 75~100cm, spacing in the rows 75~100cm.
The southern enqlish yew level land is adopted in 4~November of planting season and is manually shaded, light transmittance is less than 20%, improve extinction capability, change southern enqlish yew canopy light quality, promote the growth of southern enqlish yew, mountain region and arbor carry out the solid interplanting, utilize upper strata arbor tall trees hat to shade for southern enqlish yew provides, make it to make full use of the sylvan life scattered light, promote that biomass increases fast.
The mountain region arbor species are meant the field planting height greater than 160cm, the broad leaved and deciduous broad leaved arbor of children's year increment in age more than 110cm.
Change the shading condition in the 3rd year of the plantation of southern enqlish yew level land, and made light transmittance remain on 40%-50%, the southern enqlish yew upper strata arbor of mountain region solid growth is pruned, increase light transmittance, induce the synthetic of the interior taxol of southern enqlish yew body.
Advantage of the present invention is:
1, the present invention is according to the biological property of southern enqlish yew, initial stage highly shades to southern enqlish yew, promote southern enqlish yew to grow fast, improve biomass, before results it being carried out artificial environment coerces, taxol increases in the inductor, has promptly increased biomass and has improved content again, has promoted the output of taxol in the unit are.
2, the present invention according to the anti-shade of southern enqlish yew, utilize the strong biological property of low light level ability, carry out rational close planting, made full use of environment space on the ground, improved land utilization rate.
Embodiment:
Below enforcement of the present invention is described in further detail: belong to feminine tree according to southern enqlish yew nursery stock and young growth stage, normal growth need shade, and the characteristic that environment conditioning can induce content of taxol to improve, southern enqlish yew is shaded in early days, promote biomass to increase, changed the shading condition on the 3rd year, increase light transmittance, induce content of taxol to improve, simultaneously according to different terrain conditions, the classification plantation is taked less than 30 ° mountain region in the level land and the gradient with suiting measures to local conditions, make full use of environment space and land resources on the ground.
2, the used seedling height of described southern enqlish yew is greater than 25cm, and leading thread reaches 0.30cm, and well developed root system is preced with a complete strong sprout.
3, described southern enqlish yew plant the time then by the end of November to March in next year, before the middle ten days.
4, described southern enqlish yew adopts the seedbed mode to plant on the level land, seedbed width 150cm, and the gradient adopts solid growth less than 30 ° mountain region, banded afforestation, the afforestation band is vertical with contour, bandwidth 200~400cm.
5, described southern enqlish yew is at level land line-spacing 30~40cm, spacing in the rows 20~30cm, mountain region line-spacing 75~100cm, spacing in the rows 75~100cm.
6, described southern enqlish yew level land is adopted in 4~November of planting season and is manually built shading shed and shade, light transmittance is less than 20%, improve extinction capability, change southern enqlish yew canopy light quality, promote the growth of southern enqlish yew, mountain region and arbor carry out the solid interplanting, utilize upper strata arbor tall trees hat to shade for southern enqlish yew provides, make it to make full use of the sylvan life scattered light, promote that biomass increases fast.
7, described mountain region arbor species are meant the field planting height greater than 160cm, the broad leaved and deciduous broad leaved arbor of children's year increment in age more than 110cm.
8, change the shading condition in the 3rd year of described southern enqlish yew level land plantation, make light transmittance remain on 40%-50%, the southern enqlish yew upper strata arbor of mountain region solid growth is pruned, increase light transmittance, induce the synthetic of the interior taxol of southern enqlish yew body.
Below, the present invention will be further detailed with embodiment, but it is not limited to any or the similar example of these embodiment.
Embodiment 1:
Southern enqlish yew on ground, rice field, adopt the seedbed mode to plant, seedbed width 150cm, southern enqlish yew is planted according to seeding row spacing 20 * 30cm, used southern enqlish yew seedling height 35cm, leading thread 0.30cm, well developed root system has 5 branches, the light transmittance that shades is 11%, normal growth records southern enqlish yew average height 115cm after 3 years, stem 1.642cm fifty-fifty, and biomass is 61.2t/hm 2, changed the shading condition on the 3rd year, make light transmittance remain on 46.4%, through handling March, content of taxol on average reaches 0.104 ‰, and the southern enqlish yew biomass of growth is 6.4t/hm in the normal shading shed 2, content of taxol average out to 0.045 ‰, the content of taxol after the processing has improved 2.3 times, and biomass only differs from 4.4%.Compare with traditional planting patterns, unit are taxol output is significantly improved.
Embodiment 2:
Utilize discarded tea hill, build terraced fields, seeding row spacing according to 75 * 75cm is planted southern enqlish yew under broad leaved and deciduous broad leaved arbor camplotheca acuminata, the high growth of camplotheca acuminata annual 120cm, the living camplotheca acuminata hat width of cloth reached 220 * 230cm in 3 years, and the intensity of illumination of southern enqlish yew canopy only is 18% of vacant lot, be that the camplotheca acuminata tree crown is that 82% illumination has been gone in the southern enqlish yew screening, in April, the 3rd camplotheca acuminata leaf stretching period of plantation carries out slight beta pruning, increases the illumination of southern enqlish yew canopy, and the southern enqlish yew biomass is 8.9t/hm 2, content of taxol has reached 0.194 ‰, and the southern enqlish yew biomass of pruning without camplotheca acuminata under the similarity condition is 9.0t/hm 2, content of taxol is 0.142 ‰, average per hectare increases taxol 0.45kg.This method has not only carried out utilizing to discarded tea hill again, and has created huge economy, society and the ecological value.

Claims (8)

1, a kind of southern enqlish yew high-yield cultivating measure of directional induction taxol increment, it is characterized in that belonging to feminine tree according to southern enqlish yew nursery stock and young growth stage, normal growth need shade, and the characteristic that environment conditioning can induce content of taxol to improve, southern enqlish yew is shaded in early days, promote biomass to increase, changed the shading condition on the 3rd year, increase light transmittance, induce content of taxol to improve, according to different terrain conditions, the classification plantation is taked less than 30 ° mountain region in the level land and the gradient simultaneously with suiting measures to local conditions, make full use of environment space and land resources on the ground.
2, according to the southern enqlish yew high-yield cultivating measure of the described a kind of directional induction taxol increment of claim 1, it is characterized in that: the used seedling height of described southern enqlish yew is greater than 25cm, and leading thread reaches 0.30cm, and well developed root system is preced with a complete strong sprout.
3, according to the southern enqlish yew high-yield cultivating measure of the described a kind of directional induction taxol increment of claim 1, it is characterized in that: described southern enqlish yew plant the time then by the end of November to March in next year, before the middle ten days.
4, according to the southern enqlish yew high-yield cultivating measure of the described a kind of directional induction taxol increment of claim 1, it is characterized in that: described southern enqlish yew adopts the seedbed mode to plant on the level land, seedbed width 150cm, the gradient adopts solid growth less than 30 ° mountain region, banded afforestation, the afforestation band is vertical with contour, bandwidth 200~400cm.
5, according to the southern enqlish yew high-yield cultivating measure of the described a kind of directional induction taxol increment of claim 1, it is characterized in that: described southern enqlish yew is at level land line-spacing 30~40cm, spacing in the rows 20~30cm, mountain region line-spacing 75~100cm, spacing in the rows 75~100cm.
6, according to the southern enqlish yew high-yield cultivating measure of the described a kind of directional induction taxol increment of claim 1, it is characterized in that: described southern enqlish yew level land is adopted in 4~November of planting season and is manually shaded, light transmittance is less than 20%, improve extinction capability, change southern enqlish yew canopy light quality, promote the growth of southern enqlish yew, mountain region and arbor carry out the solid interplanting, utilize upper strata arbor tall trees hat to shade for southern enqlish yew provides, make it to make full use of the sylvan life scattered light, promote that biomass increases fast.
7, according to the southern enqlish yew high-yield cultivating measure of the described a kind of directional induction taxol increment of claim 1, it is characterized in that: described mountain region arbor species are meant the field planting height greater than 160cm, the broad leaved and deciduous broad leaved arbor of children's year increment in age more than 110cm.
8, according to the southern enqlish yew high-yield cultivating measure of the described a kind of directional induction taxol increment of claim 1, it is characterized in that: the 3rd year change shading condition of described southern enqlish yew level land plantation, make light transmittance remain on 40%-50%, southern enqlish yew upper strata arbor to the mountain region solid growth is pruned, increase light transmittance, induce the synthetic of the interior taxol of southern enqlish yew body.
CNB2006100105193A 2006-09-08 2006-09-08 Steps for breeding high production southern yew to increase pacilitaxel through directional inducement Active CN100452964C (en)

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Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102067809A (en) * 2010-11-18 2011-05-25 华侨大学 Method for three-dimensionally ecologically cultivating eucalypts and taxus chinensis var. mairei
CN103299820A (en) * 2013-07-08 2013-09-18 中国林业科学研究院资源昆虫研究所 Culturing method for improving content of paclitaxel in taxus chinensis
CN103404354A (en) * 2013-08-21 2013-11-27 江苏红豆杉生物科技股份有限公司 Planting method of Taxus chinensis var mairei
CN104094765B (en) * 2014-07-28 2016-06-01 重庆市碚圣农业科技股份有限公司 A kind of cultivation and collection method improving Taxus x media biomass
CN105123380A (en) * 2015-07-21 2015-12-09 邹小龙 Chinese yew breeding method for improving paclitaxel content of Chinese yew
CN111492885B (en) 2020-05-14 2021-06-22 东北林业大学 Method for building mixed forest of Chinese yew and fig trees
CN115812509B (en) * 2022-12-27 2023-08-11 延边大学 Multifunctional composite landscape plant ecological planting method

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CN1910994A (en) * 2006-08-22 2007-02-14 东北林业大学 Method for three-D cultivation of camptotheca acuminata, Chinese yew and rosemary

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Publication number Priority date Publication date Assignee Title
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CN1910994A (en) * 2006-08-22 2007-02-14 东北林业大学 Method for three-D cultivation of camptotheca acuminata, Chinese yew and rosemary

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