CN105123380A - Chinese yew breeding method for improving paclitaxel content of Chinese yew - Google Patents
Chinese yew breeding method for improving paclitaxel content of Chinese yew Download PDFInfo
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- CN105123380A CN105123380A CN201510429862.0A CN201510429862A CN105123380A CN 105123380 A CN105123380 A CN 105123380A CN 201510429862 A CN201510429862 A CN 201510429862A CN 105123380 A CN105123380 A CN 105123380A
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- chinese yew
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
Abstract
The invention discloses a Chinese yew breeding method for improving paclitaxel content of a Chinese yew. The method comprises the following steps: 1) breeding seeds: each year from March to April, in desolate mountain, pulling fresh and alive and robustly growing moso bamboo to cover a Chinese yew, applying UV-irradiation, using a bee pollination copulation method to breed a natural wild millennium Chinese yew seed tree, after taking care of the tree for more than ten months in the desolate mountain, the fruits of the redwood tree fully absorbing various kinds of benefical natural elements and nutrients of air and the moso bamboo, through a pollen period, a green fruit period, fruits being mature in a red bright period, the mature period being in the middle of Chinese calendar October, harvesting in autumn to obtain seeds of mature Chinese yew; 2) cellaring the seeds, including performing pre-treatment on the seeds, and cellaring to dormancy for three years; 3) growing, including screening full seeds to directly seeding in seedling furrow land, and the seeds naturally sprouting to breed a Chinese yew sapling. The method solves problems in the prior art that Chinese yew resources are wasted, and original values of a Chinese yew species are improved.
Description
Technical field
The present invention relates to the propagation method of a kind of Chinese yew, concrete, relate to a kind of Chinese yew propagation method improving taxus brevifolia alcohol content, belong to plant propagation culture technique field.
Background technology
Chinese yew is a kind of seeds of mystery, is once brought into space by No. four, divine boat, and in White House, Buckingham Palace, England, the General Headquarters of the United Nations can find its beautiful image, and it is exactly the Chinese yew being described as healthy tree.
Why there is so high status, all come from the eight large features that it has.
1. national treasure:
Chinese yew be carried over 2,500,000 years the 4th glacial epoch in century treasure species of frequently endangering; it is the living fossil in plant; within 1994, Chinese yew is first class of protection plant by man of China, and it is plant giant panda worthy of the name that 42, the whole world has the country of Chinese yew to be all called " national treasure ".
2. Taxus CAM class plant:
Chinese yew whole day 24 hours sucks carbonic acid gas, and the advantage maximum compared with other plant of exhalation oxygen is adapted at indoor placement, plays oxygenation effect.
3. absorb toxic and harmful:
Chinese yew not only can absorb carbon monoxide, and sulphur dioxide waits noxious material, can also absorb formaldehyde, benzene, toluene, the carcinogens such as dimethylbenzene, can purify air, play protective effect on cancer risk.
4. containing cancer-resisting substance taxol:
Chinese yew is the species that uniquely can extract taxol in world's all things on earth, and taxol is internationally recognized carcinogenic good medicine, and wide spectrum low toxicity is efficient.
5. lucky tree:
Chinese yew number can reach more than 5000 age, is called " hollywood lignumvitae ", the full branch of its haw, and glittering and translucent, implied meaning luckiness is celebrating, so be referred to as again " lucky tree ".
6. eucalyptus:
Taxus whole body is precious, and its material body is the redwood of high-quality, and can do top-grade furniture, fruit can cook health products, and root can make handicraft, and skin and radicula branches and leaves are the best raw materials of taxol, so be otherwise known as " eucalyptus ".
7. resistance to cloudy heatproof:
Chinese yew is fabulous potted plant ornamental foliage plant, and its happiness dark and damply keeps away sunlight, waters less, fabulous maintenance, and when temperatures as high 41 DEG C, still well-grown, temperature can also micro-length subzero 6 degrees Celsius time, evergreen all the year round, handsome in appearance, is the suitable evergreen plant in north and south.
8. forfend mosquito:
Taxus passes through photosynthesis, the scent molecule citronellal of outside volatilization oneself has the effect forfending mosquito, temperature is higher, its more repelling effect of fragrant molecule played is better, and it is this pure and fresh simple and elegant, hot summer Bracing, relaxed and joyful, testing this fragrance through authority can hundreds of mosquito of repellent to person poultry harmless.
Expert claims, and has resistance to cloudy heatproof simultaneously, and the plant belonging to CAM class is little, adds the function of Chinese yew uniqueness, can be beautiful with its castor without any plant.
Yew industry is health industry, for conservation culture contributes, thus, greatly develops the breeding of Chinese yew, benefits the nation and the people, promote the well-being of mankind.
Summary of the invention
The technical issues that need to address of the present invention crack the fruit germination hastening method breeding then of existing Chinese yew, a kind of Chinese yew propagation method improving taxus brevifolia alcohol content is provided, it solve prior art waste taxus resource problem, improve original value of Chinese yew species.
The technical scheme that the present invention solve the technical problem employing is:
A kind of Chinese yew propagation method improving taxus brevifolia alcohol content of the present invention, described method comprises the following steps:
1) cultivating seeds: annual 3-4 month, in desolate mountain, Chinese yew is hidden with mao bamboon traction that is fresh and alive, that grow up healthy and strong, carry out ultraviolet lighting, carry out honeybee pollination mating method to wild Chinese yew elite stand in thousand to cultivate, the care of more than ten month is gone through in desolate mountain, fire works of sequoia tree fruit is allowed fully to absorb multiple beneficial native element and the nutrient of air and mao bamboon, through pollen period, Chinese olive phase, to the red bright phase, i.e. fruit maturation, the maturing stage is in lunar calendar mid-October, autumn gathers, and obtains ripe Chinese yew seed;
2), seed stores sth. in a cellar: the pre-treatment carrying out seed, stores sth. in a cellar dormancy 3 years;
The pre-treatment of seed is:
One, the lunar calendar October, select the good sky of the clear and crisp days of autumn to carry out seed harvesting, every frame can not be transported to seed pre-treatment workshop and spread out on ground more than 10 kilograms;
Two, core is got in peeling, the seed being spread out in ground, puts into container and adds water, and manually with the hands rub with the hands and go the crust of seed to get core, Exposure to Sunlight one day, cool place is stored sth. in a cellar for dry one week;
Described store sth. in a cellar for: burrow on the hillside faced south cave 5-6 square, under cellar for storing things, pad fine sand about a meter is dark, air adjustment solar term and temperature automatically controlled wet instrument on cellar for storing things is in-built, temperature is transferred to 16 DEG C, humidity 30%, then the seed of drying is put into fine sand, and neat and well spaced flat, put insect-and mouse-preventing medicine and store sth. in a cellar sealing 3 years; Annual cellar for storing things of regularly carrying out out for 4-5 time is turned over even inspection and is sealed.
3) grow: the live seedling ditch ground of screening full seed, naturally germinate and be bred as Chinese yew sapling.
After 1 year by the Chinese yew transplantation of seedlings that survives to land for growing field crops self-sow; After 3 years, the Taxus little tree of with luxuriant foliage and spreading branches in leafy profusion stalwartness is transplanted to desolate mountain self-sow to grow into forest.
The Chinese yew of the present invention's cultivation can significantly improve the content of taxol in branches and leaves of yew, can be beautiful with Taxus x media castor, and in the dry China fir branches and leaves of Chinese yew of the present invention's cultivation by analysis, content of taxol is 0.012%, RSD is 1.46%.Become the excellent raw material of spy that the world today extracts taxol.Taxol is current first-selected widely used a kind of cancer therapy drug in the world.
Embodiment
A kind of Chinese yew propagation method improving taxus brevifolia alcohol content of the present invention, described method comprises the following steps:
1) cultivating seeds: annual 3-4 month, in desolate mountain, Chinese yew is hidden with mao bamboon traction that is fresh and alive, that grow up healthy and strong, carry out ultraviolet lighting, carry out honeybee pollination mating method to wild Chinese yew elite stand in thousand to cultivate, the care of more than ten month is gone through in desolate mountain, fire works of sequoia tree fruit is allowed fully to absorb multiple beneficial native element and the nutrient of air and mao bamboon, through pollen period, Chinese olive phase, to the red bright phase, i.e. fruit maturation, the maturing stage is in lunar calendar mid-October, autumn gathers, and obtains ripe Chinese yew seed;
2), seed stores sth. in a cellar: the pre-treatment carrying out seed, stores sth. in a cellar dormancy 3 years;
The pre-treatment of seed is:
One, the lunar calendar October, select the good sky of the clear and crisp days of autumn to carry out seed harvesting, every frame can not be transported to seed pre-treatment workshop and spread out on ground more than 10 kilograms;
Two, core is got in peeling, the seed being spread out in ground, puts into container and adds water, and manually with the hands rub with the hands and go the crust of seed to get core, Exposure to Sunlight one day, cool place is stored sth. in a cellar for dry one week;
Described store sth. in a cellar for: burrow on the hillside faced south cave 5-6 square, under cellar for storing things, pad fine sand about a meter is dark, air adjustment solar term and temperature automatically controlled wet instrument on cellar for storing things is in-built, temperature is transferred to 16 DEG C, humidity 30%, then the seed of drying is put into fine sand, and neat and well spaced flat, put insect-and mouse-preventing medicine and store sth. in a cellar sealing 3 years; Annual cellar for storing things of regularly carrying out out for 4-5 time is turned over even inspection and is sealed.
3) grow: the live seedling ditch ground of screening full seed, naturally germinate and be bred as Chinese yew sapling.
After 1 year by the Chinese yew transplantation of seedlings that survives to land for growing field crops self-sow; After 3 years, the Taxus little tree of with luxuriant foliage and spreading branches in leafy profusion stalwartness is transplanted to desolate mountain self-sow to grow into forest.
The Chinese yew of the present invention's cultivation can significantly improve the content of taxol in branches and leaves of yew, can be beautiful with Taxus x media castor, and in the dry China fir branches and leaves of Chinese yew of the present invention's cultivation by analysis, content of taxol is 0.012%, RSD is 1.46%.Become the excellent raw material of spy that the world today extracts taxol.Taxol is current first-selected widely used a kind of cancer therapy drug in the world.
Last it is noted that obviously, above-described embodiment is only for example of the present invention is clearly described, and the restriction not to embodiment.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here exhaustive without the need to also giving all embodiments.And thus the apparent change of amplifying out or variation be still among protection scope of the present invention.
Claims (2)
1. improve a Chinese yew propagation method for taxus brevifolia alcohol content, it is characterized in that, described method comprises the following steps:
1) cultivating seeds: annual 3-4 month, in desolate mountain, Chinese yew is hidden with mao bamboon traction that is fresh and alive, that grow up healthy and strong, carry out ultraviolet lighting, carry out honeybee pollination mating method to wild Chinese yew elite stand in thousand to cultivate, the care of more than ten month is gone through in desolate mountain, fire works of sequoia tree fruit is allowed fully to absorb multiple beneficial native element and the nutrient of air and mao bamboon, through pollen period, Chinese olive phase, to the red bright phase, i.e. fruit maturation, the maturing stage is in lunar calendar mid-October, autumn gathers, and obtains ripe Chinese yew seed;
2), seed stores sth. in a cellar: the pre-treatment carrying out seed, stores sth. in a cellar dormancy 3 years;
The pre-treatment of seed is:
One, the lunar calendar October, select the good sky of the clear and crisp days of autumn to carry out seed harvesting, every frame can not be transported to seed pre-treatment workshop and spread out on ground more than 10 kilograms;
Two, core is got in peeling, the seed being spread out in ground, puts into container and adds water, and manually with the hands rub with the hands and go the crust of seed to get core, Exposure to Sunlight one day, cool place is stored sth. in a cellar for dry one week;
Described store sth. in a cellar for: burrow on the hillside faced south cave 5-6 square, under cellar for storing things, pad fine sand about a meter is dark, air adjustment solar term and temperature automatically controlled wet instrument on cellar for storing things is in-built, temperature is transferred to 16 DEG C, humidity 30%, then the seed of drying is put into fine sand, and neat and well spaced flat, put insect-and mouse-preventing medicine and store sth. in a cellar sealing 3 years; Annual cellar for storing things of regularly carrying out out for 4-5 time is turned over even inspection and is sealed;
3) grow: the live seedling ditch ground of screening full seed, naturally germinate and be bred as Chinese yew sapling.
2. the as claimed in claim 1 Chinese yew propagation method improving taxus brevifolia alcohol content, is characterized in that, after 1 year by the Chinese yew transplantation of seedlings that survives to land for growing field crops self-sow; After 3 years, the Taxus little tree of with luxuriant foliage and spreading branches in leafy profusion stalwartness is transplanted to desolate mountain self-sow to grow into forest.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510429862.0A CN105123380A (en) | 2015-07-21 | 2015-07-21 | Chinese yew breeding method for improving paclitaxel content of Chinese yew |
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| CN201510429862.0A CN105123380A (en) | 2015-07-21 | 2015-07-21 | Chinese yew breeding method for improving paclitaxel content of Chinese yew |
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| CN201510429862.0A Pending CN105123380A (en) | 2015-07-21 | 2015-07-21 | Chinese yew breeding method for improving paclitaxel content of Chinese yew |
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1615682A (en) * | 2004-10-21 | 2005-05-18 | 李万祥 | Method for planting Chinese yew |
| CN1914994A (en) * | 2006-09-08 | 2007-02-21 | 浙江海正药业股份有限公司 | Steps for breeding high production southern yew to increase pacilitaxel through directional inducement |
| CN101147453A (en) * | 2007-11-01 | 2008-03-26 | 东北林业大学 | Artificial cultivation method for taxus chinenesis containing high content taxol |
| CN101578955A (en) * | 2009-06-05 | 2009-11-18 | 张鹏 | Cultivation and collection method for improving taxol content in taxus media branches and leaves |
| CN102067809A (en) * | 2010-11-18 | 2011-05-25 | 华侨大学 | Method for three-dimensionally ecologically cultivating eucalypts and taxus chinensis var. mairei |
| CN102498853A (en) * | 2011-10-21 | 2012-06-20 | 江苏红豆杉生物科技有限公司 | Planting and harvesting method for improving 10-DABIII content of artificially planted laxus malrel |
| CN103004440A (en) * | 2012-12-27 | 2013-04-03 | 曲靖市民丰现代农业科技开发有限公司 | Stereoscopic ecological cultivation method of Taxus yunnanensis and walnut |
| CN103299820A (en) * | 2013-07-08 | 2013-09-18 | 中国林业科学研究院资源昆虫研究所 | Culturing method for improving content of paclitaxel in taxus chinensis |
-
2015
- 2015-07-21 CN CN201510429862.0A patent/CN105123380A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1615682A (en) * | 2004-10-21 | 2005-05-18 | 李万祥 | Method for planting Chinese yew |
| CN1914994A (en) * | 2006-09-08 | 2007-02-21 | 浙江海正药业股份有限公司 | Steps for breeding high production southern yew to increase pacilitaxel through directional inducement |
| CN101147453A (en) * | 2007-11-01 | 2008-03-26 | 东北林业大学 | Artificial cultivation method for taxus chinenesis containing high content taxol |
| CN101578955A (en) * | 2009-06-05 | 2009-11-18 | 张鹏 | Cultivation and collection method for improving taxol content in taxus media branches and leaves |
| CN102067809A (en) * | 2010-11-18 | 2011-05-25 | 华侨大学 | Method for three-dimensionally ecologically cultivating eucalypts and taxus chinensis var. mairei |
| CN102498853A (en) * | 2011-10-21 | 2012-06-20 | 江苏红豆杉生物科技有限公司 | Planting and harvesting method for improving 10-DABIII content of artificially planted laxus malrel |
| CN103004440A (en) * | 2012-12-27 | 2013-04-03 | 曲靖市民丰现代农业科技开发有限公司 | Stereoscopic ecological cultivation method of Taxus yunnanensis and walnut |
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Application publication date: 20151209 |
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